                         SEQUENCE LISTING

<110>  UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND 
       CORK
 
<120>  An omega-transaminase enzyme

<130>  P12496PC00

<150>  EP 18186426.5
<151>  2018-07-30

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  463
<212>  PRT
<213>  Pseudovibrio

<400>  1

Met Asp Tyr Ile Ala Asn Leu Pro Pro Thr His Val Leu Gln Glu Lys 
1               5                   10                  15      


Asp Ala Ala His His Leu His Pro Phe Thr Asp Thr Lys Ser Leu Asn 
            20                  25                  30          


Ala Lys Gly Thr Arg Val Ile Thr Arg Ala Asp Gly Val Tyr Leu Trp 
        35                  40                  45              


Asp Ser Glu Gly Asn Lys Ile Leu Asp Gly Met Ala Gly Leu Trp Cys 
    50                  55                  60                  


Val Asn Val Gly Tyr Gly Arg Gln Glu Ile Ile Asp Ala Val Tyr Arg 
65                  70                  75                  80  


Gln Met Gln Gln Leu Pro Tyr Tyr Asn Thr Phe Phe Gln Ser Ser His 
                85                  90                  95      


Pro Pro Ala Ile Gly Leu Ala Glu Arg Ile Ser Ser Leu Ala Pro Asp 
            100                 105                 110         


His Leu Asp His Val Phe Phe Ala Gly Ser Gly Ser Glu Ala Asn Asp 
        115                 120                 125             


Thr Val Val Arg Met Val Arg His Tyr Trp Ala Ser Glu Gly Lys Pro 
    130                 135                 140                 


Thr Lys Lys Thr Ile Ile Ser Arg His Asn Ala Tyr His Gly Ser Thr 
145                 150                 155                 160 


Met Ala Gly Ala Ser Leu Gly Gly Met Ser Ala Met His Ala Gln Gly 
                165                 170                 175     


Gly Leu Pro Ile Pro Asp Ile Thr His Ile Asn Gln Pro Tyr Trp Tyr 
            180                 185                 190         


Gly Glu Gly Gly Asp Met Asp Pro Ala Ala Phe Gly Leu Met Arg Ala 
        195                 200                 205             


Arg Glu Leu Glu Ala Glu Ile Asp Arg Leu Gly Glu Asp Asn Val Ala 
    210                 215                 220                 


Ala Phe Ile Gly Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Ile Pro 
225                 230                 235                 240 


Pro Glu Thr Tyr Trp Pro Glu Ile Gln Arg Ile Cys Arg Glu Arg Asn 
                245                 250                 255     


Ile Leu Leu Ile Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly 
            260                 265                 270         


Asn Trp Phe Gly Ser Gln Thr Phe Asn Phe Lys Pro Asp Leu Met Pro 
        275                 280                 285             


Ile Ala Lys Gly Leu Ser Ser Gly Tyr Leu Pro Ile Gly Ala Val Val 
    290                 295                 300                 


Val Ser Glu Lys Val Ala Lys Gly Phe Ile Glu His Gly Gly Glu Phe 
305                 310                 315                 320 


Tyr His Gly Phe Thr Tyr Ser Ala His Pro Ala Ala Cys Ala Ala Ala 
                325                 330                 335     


Leu Ala Asn Leu Asp Ile Ile Glu Asn Glu Arg Leu Pro Glu Lys Val 
            340                 345                 350         


Ala Asn Asp Thr Gly Pro Tyr Leu Ala Glu Lys Trp Lys Thr Leu Gly 
        355                 360                 365             


Glu His Pro Leu Val Gly Glu Ala Arg Ile Cys Gly Leu Val Gly Ala 
    370                 375                 380                 


Leu Glu Leu Ser Pro Asp Lys Ala Arg Arg Ala Arg Phe Glu Ala Glu 
385                 390                 395                 400 


Lys Gly Thr Val Gly Thr Ile Cys Arg Asp His Cys Phe Glu Ser Gly 
                405                 410                 415     


Leu Val Met Arg His Val Gly Asp Ser Met Ile Ile Ser Pro Pro Leu 
            420                 425                 430         


Val Ile Ser Arg Ser Glu Val Asp Glu Leu Ile Gln Lys Ala His Arg 
        435                 440                 445             


Ala Leu Asp Leu Thr Ala Ala Asp Val Thr Ala Gln Ser Ile Lys 
    450                 455                 460             


<210>  2
<211>  1392
<212>  DNA
<213>  Pseudovibrio

<400>  2
atggactata tcgctaatct tccgcccact catgttctgc aagaaaagga tgccgcccac       60

catttgcatc cgtttacaga taccaagtcc ctcaacgcca aagggacgcg tgttattacg      120

cgggctgatg gtgtctatct ttgggattct gagggcaaca agattctgga tggtatggcc      180

gggctttggt gcgtgaacgt ggggtatggc cgtcaagaga ttatcgatgc ggtctaccgg      240

caaatgcaac aactgccgta ttacaatacg ttttttcaga gttctcaccc gcctgcaatt      300

ggtcttgctg aaaggatctc cagtctggca ccagatcatc tggaccatgt gttctttgca      360

ggctcgggct ctgaagccaa tgacaccgtg gtgcggatgg tgcgccatta ctgggctagt      420

gaaggtaagc cgaccaagaa gacaatcatt tcccgtcata atgcttatca cggctccact      480

atggctggtg cgagcttggg tggtatgagt gcgatgcatg cacagggcgg tctacctatt      540

cctgacatta cccatatcaa ccagccttat tggtatggtg aaggcggaga tatggaccct      600

gccgcgtttg gactgatgcg tgcacgtgag ctggaagctg agattgaccg gcttggagaa      660

gacaatgtag cggcctttat cggggagcct attcagggag cgggtggtgt gatcattccg      720

ccagaaacat attggcccga gattcagcgt atctgccgtg aacgcaatat tcttctgatt      780

gctgatgaag tgatttgcgg gtttgggcgt acgggtaact ggtttgggtc acagactttc      840

aacttcaaac cggatctgat gccgattgcc aaggggcttt cctcaggtta tctgccgatt      900

ggtgcggttg tggtgagtga gaaggttgcc aagggcttta ttgagcacgg cggtgaattt      960

tatcatggtt tcacttactc ggcacatccg gcagcttgtg ccgcagcact tgcgaacctt     1020

gatattattg agaatgagcg actgccggaa aaagtggcga atgatacagg gccgtatcta     1080

gctgagaaat ggaagactct tggcgagcat ccgttggttg gtgaggcgcg tatctgcggg     1140

ctggttggtg ctttggaact ttcgcctgat aaggcacgcc gtgctcgttt tgaagctgaa     1200

aaaggcactg ttggaacgat ctgccgcgat cattgctttg agagcggttt ggtgatgcgc     1260

catgttggcg acagcatgat catctcaccg ccacttgtga tctcgcgcag tgaggtggat     1320

gagttgattc agaaagcgca tcgtgcgttg gatctgacgg ctgctgatgt tactgcccaa     1380

agcatcaaat aa                                                         1392


<210>  3
<211>  463
<212>  PRT
<213>  Pseudovibrio

<400>  3

Met Asp Tyr Ile Ala Asn Leu Pro Pro Thr His Val Leu Gln Lys Lys 
1               5                   10                  15      


Asp Ala Ala His His Leu His Pro Phe Thr Asp Thr Gln Ser Leu Asn 
            20                  25                  30          


Ala Lys Gly Thr Arg Ile Ile Thr Arg Ala Glu Gly Val Tyr Leu Trp 
        35                  40                  45              


Asp Ser Glu Gly Asn Lys Ile Leu Asp Gly Met Ala Gly Leu Trp Cys 
    50                  55                  60                  


Val Asn Val Gly Tyr Gly Arg Lys Glu Ile Ile Glu Ala Val His Arg 
65                  70                  75                  80  


Gln Met Gln Gln Leu Pro Tyr Tyr Asn Thr Phe Phe Gln Thr Ser His 
                85                  90                  95      


Pro Pro Ala Ile Ala Leu Ala Glu Lys Ile Ala Lys Leu Ala Pro Gly 
            100                 105                 110         


Asp Leu Asp Tyr Val Phe Phe Ala Gly Ser Gly Ser Glu Ala Asn Asp 
        115                 120                 125             


Thr Val Val Arg Met Val Arg His Tyr Trp Ala Ser Lys Gly Lys Pro 
    130                 135                 140                 


Thr Lys Lys Thr Ile Ile Ser Arg His Asn Ala Tyr His Gly Ser Thr 
145                 150                 155                 160 


Met Ala Gly Ala Ser Leu Gly Gly Met Ser Ala Met His Ala Gln Gly 
                165                 170                 175     


Gly Leu Pro Ile Pro Asp Ile Thr His Ile Asn Gln Pro Tyr Trp Tyr 
            180                 185                 190         


Ala Glu Gly Gly Asp Met Asp Pro Ala Glu Phe Gly Leu Ala Arg Ala 
        195                 200                 205             


Arg Glu Leu Glu Gln Glu Ile Glu Arg Leu Gly Ala Asp Asn Val Ala 
    210                 215                 220                 


Ala Phe Ile Gly Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Ile Pro 
225                 230                 235                 240 


Pro Glu Thr Tyr Trp Pro Glu Ile Gln Arg Ile Cys Arg Glu His Asp 
                245                 250                 255     


Ile Leu Leu Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly 
            260                 265                 270         


His Trp Phe Gly Ser Gln Thr Phe Gly Phe Lys Pro Asp Leu Met Pro 
        275                 280                 285             


Ile Ala Lys Gly Leu Ser Ser Gly Tyr Leu Pro Ile Gly Ala Val Val 
    290                 295                 300                 


Met Ser Glu Asp Val Ala Gln Gly Phe Ile Thr His Gly Gly Glu Phe 
305                 310                 315                 320 


Cys His Gly Phe Thr Tyr Ser Ala His Pro Ser Ala Cys Ala Ala Ala 
                325                 330                 335     


Leu Ala Asn Leu Glu Ile Leu Glu Ser Glu Arg Leu Pro Ala Lys Val 
            340                 345                 350         


Ala Thr Asp Thr Gly Pro Tyr Leu Ala Glu Arg Trp Gln Thr Leu Ala 
        355                 360                 365             


Glu His Pro Leu Val Gly Glu Thr Arg Ile Cys Gly Leu Ile Gly Ala 
    370                 375                 380                 


Leu Glu Leu Ser Pro Asp Lys Lys Arg Arg Ala Pro Phe Glu Ala Glu 
385                 390                 395                 400 


Arg Gly Thr Val Gly Thr Ile Cys Arg Asp His Cys Phe Ala Thr Gly 
                405                 410                 415     


Leu Val Met Arg His Val Gly Asp Thr Met Val Ile Ser Pro Pro Leu 
            420                 425                 430         


Val Ile Ser His Ser Glu Val Asp Glu Leu Ile Glu Lys Val His Arg 
        435                 440                 445             


Ala Leu Asp Leu Thr Ser Ala Asp Ile Ala Leu Gln Gly Ile Lys 
    450                 455                 460             


<210>  4
<211>  1392
<212>  DNA
<213>  Pseudovibrio

<400>  4
atggactaca ttgccaatct tccgccaaca catgtgttgc agaaaaagga tgccgctcat       60

catctgcatc cctttactga tacccagagc cttaatgcaa aaggcacgcg gattattacg      120

cgagctgaag gcgtgtattt gtgggactct gaaggcaata aaattcttga tggaatggcc      180

ggtctttggt gcgtgaatgt tgggtatggc cgaaaagaga tcatcgaagc cgttcatcgc      240

cagatgcagc agctgcccta ctacaataca tttttccaga catcacaccc tccggcgatt      300

gccctggcag aaaagattgc aaagcttgca cctggagacc ttgattatgt gttctttgca      360

ggttctggtt ctgaggcgaa tgatacagtg gtgcgtatgg tgcgtcatta ctgggccagc      420

aaaggtaaac cgaccaagaa aaccataatt tcccgacata atgcctatca tggttcaacg      480

atggcggggg cgagccttgg cggcatgagt gctatgcatg cccaaggtgg attgcccatc      540

ccagatatca cccatatcaa tcagccttac tggtatgcag aaggcgggga catggacccg      600

gcagaattcg ggttggcacg ggcgcgcgag ttagaacagg aaattgaacg actgggtgcg      660

gacaatgtgg cggcatttat tggtgagcct atacaagggg caggtggggt tattattccg      720

cctgaaacgt actggcctga aattcagcgg atttgccgcg agcacgatat cttgctggtt      780

gctgatgaag tcatttgtgg ctttgggcgc acgggtcact ggtttggttc gcaaacattt      840

ggcttcaaac ctgacctgat gccaatcgca aaaggcctat cctctggtta tctgccgatt      900

ggtgctgtgg tgatgagtga agatgttgcc cagggtttca tcacacatgg tggtgagttt      960

tgtcatggct ttacgtattc tgcacatcct tcggcttgtg cagcggcgtt ggctaacctg     1020

gaaattcttg agagtgaacg tttgcctgca aaagttgcga ctgatacagg tccgtatctt     1080

gcagaacgat ggcagacact ggctgagcac ccgcttgtcg gtgaaacgcg catttgtggt     1140

ttgatcggtg cgttggagct ttcacccgac aaaaaacgtc gcgctccctt tgaagcggaa     1200

aggggcactg tgggcaccat ctgccgtgat cattgctttg caacggggct ggtgatgcgc     1260

catgtgggag atacgatggt tatttcgcct ccgcttgtca tttctcactc agaagtggat     1320

gagctgatcg agaaagtaca tcgggcattg gacctgactt cagcggatat tgcgcttcaa     1380

gggatcaagt ag                                                         1392


<210>  5
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BamPTAF primer

<400>  5
gaaggatcca tggactatat cgctaattct tccg                                   34


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XhoPTAR primer

<400>  6
cgtcctcgag ttatttgatg ctttgggcag t                                      31


<210>  7
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BamCTAF primer

<400>  7
gcgggatcca tgcagaagca acgtacgacc a                                      31


<210>  8
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XhoCTAR primer

<400>  8
ctatctcgag actaagccag cccgcgcgcc ttc                                    33


