               SEQUENCE LISTING

<110> Rijk Zwaan Zaadteelt en Zaadhandel B.V.

<120> Mutant Allele Of The ACO2 Gene

<130> P125747PC01

<140> 
<141> 

<160> 6

<170> BiSSAP 1.3.6

<210> 1
<211> 963
<212> DNA
<213> Cucumis sativus


<220> 
<223> Wildtype ACO2

<400> 1
atggagatgg atttccctgt catcaacatg aacaacctca atggcgaaag cagagtatct      60

gtcctgaacc aaatcaacga cgcctgcgaa aactggggtt tttttgagtt ggtgaatcat     120

gggataccac atgagctgat ggacaaagtg gagaagatga caaaggaaca ttatagaaag     180

tgcatggagc agaggtttaa agaaatggta gcttccaaag ggttggattc agtggaaact     240

gagatcaacg acactgattg ggaaagcact ttttttttac gccatcttcc agtttcaaac     300

atgtcagaaa ttggtgatct ggatgaggag tacaagaagg tgatgaagga atttgcagct     360

gaactggaga aattagcgga ggaaattctg aacttgttgt gtgagaatct tgggcttgaa     420

aaggggtatt tgaaaaaagt gttttatgga tcaaaaggcc caaactttgg gacaaaagtt     480

agcaattacc ctccatgtcc taaacctgag cttattaaag gacttagagc ccataccgat     540

gctggtggtc ttattcttct gtttcaagat gataaagtga gtgggcttca gctgctcaaa     600

gatggaaaat gggttgatgt tcctccgatg catcactcca ttgttatcaa tttaggagac     660

cagcttgaag taataacaaa tggaaaatac aagagtgtaa tgcacagagt gatagcacag     720

gaggatggaa acagaatgtc gatagcatca ttctacaatc caggaaacga cgccgtaatc     780

tatccggcgc cggcgctggt cgagggagag caggagaaaa ccaaacttta cccaaaattt     840

gtgttcgatg attacatgaa gctgtatatg gggcttaagt ttcaagcaaa agagccaagg     900

tttgaggcca tgaaagccat ggagtccacc aatatcaata tgggtccaat tgcaactgtc     960

tga                                                                   963


<210> 2
<211> 320
<212> PRT
<213> Cucumis sativus


<220> 
<223> Wildtype ACO2

<400> 2
Met Glu Met Asp Phe Pro Val Ile Asn Met Asn Asn Leu Asn Gly Glu 
1               5                   10                  15      
Ser Arg Val Ser Val Leu Asn Gln Ile Asn Asp Ala Cys Glu Asn Trp 
            20                  25                  30          
Gly Phe Phe Glu Leu Val Asn His Gly Ile Pro His Glu Leu Met Asp 
        35                  40                  45              
Lys Val Glu Lys Met Thr Lys Glu His Tyr Arg Lys Cys Met Glu Gln 
    50                  55                  60                  
Arg Phe Lys Glu Met Val Ala Ser Lys Gly Leu Asp Ser Val Glu Thr 
65                  70                  75                  80  
Glu Ile Asn Asp Thr Asp Trp Glu Ser Thr Phe Phe Leu Arg His Leu 
                85                  90                  95      
Pro Val Ser Asn Met Ser Glu Ile Gly Asp Leu Asp Glu Glu Tyr Lys 
            100                 105                 110         
Lys Val Met Lys Glu Phe Ala Ala Glu Leu Glu Lys Leu Ala Glu Glu 
        115                 120                 125             
Ile Leu Asn Leu Leu Cys Glu Asn Leu Gly Leu Glu Lys Gly Tyr Leu 
    130                 135                 140                 
Lys Lys Val Phe Tyr Gly Ser Lys Gly Pro Asn Phe Gly Thr Lys Val 
145                 150                 155                 160 
Ser Asn Tyr Pro Pro Cys Pro Lys Pro Glu Leu Ile Lys Gly Leu Arg 
                165                 170                 175     
Ala His Thr Asp Ala Gly Gly Leu Ile Leu Leu Phe Gln Asp Asp Lys 
            180                 185                 190         
Val Ser Gly Leu Gln Leu Leu Lys Asp Gly Lys Trp Val Asp Val Pro 
        195                 200                 205             
Pro Met His His Ser Ile Val Ile Asn Leu Gly Asp Gln Leu Glu Val 
    210                 215                 220                 
Ile Thr Asn Gly Lys Tyr Lys Ser Val Met His Arg Val Ile Ala Gln 
225                 230                 235                 240 
Glu Asp Gly Asn Arg Met Ser Ile Ala Ser Phe Tyr Asn Pro Gly Asn 
                245                 250                 255     
Asp Ala Val Ile Tyr Pro Ala Pro Ala Leu Val Glu Gly Glu Gln Glu 
            260                 265                 270         
Lys Thr Lys Leu Tyr Pro Lys Phe Val Phe Asp Asp Tyr Met Lys Leu 
        275                 280                 285             
Tyr Met Gly Leu Lys Phe Gln Ala Lys Glu Pro Arg Phe Glu Ala Met 
    290                 295                 300                 
Lys Ala Met Glu Ser Thr Asn Ile Asn Met Gly Pro Ile Ala Thr Val 
305                 310                 315                 320 



<210> 3
<211> 963
<212> DNA
<213> Cucumis sativus


<220> 
<223> Mutant ACO2

<220> 
<221> misc_feature
<222> 278
<223> /note="n = a or t or c or g"

<400> 3
atggagatgg atttccctgt catcaacatg aacaacctca atggcgaaag cagagtatct      60

gtcctgaacc aaatcaacga cgcctgcgaa aactggggtt tttttgagtt ggtgaatcat     120

gggataccac atgagctgat ggacaaagtg gagaagatga caaaggaaca ttatagaaag     180

tgcatggagc agaggtttaa agaaatggta gcttccaaag ggttggattc agtggaaact     240

gagatcaacg acactgattg ggaaagcact tttttttnac gccatcttcc agtttcaaac     300

atgtcagaaa ttggtgatct ggatgaggag tacaagaagg tgatgaagga atttgcagct     360

gaactggaga aattagcgga ggaaattctg aacttgttgt gtgagaatct tgggcttgaa     420

aaggggtatt tgaaaaaagt gttttatgga tcaaaaggcc caaactttgg gacaaaagtt     480

agcaattacc ctccatgtcc taaacctgag cttattaaag gacttagagc ccataccgat     540

gctggtggtc ttattcttct gtttcaagat gataaagtga gtgggcttca gctgctcaaa     600

gatggaaaat gggttgatgt tcctccgatg catcactcca ttgttatcaa tttaggagac     660

cagcttgaag taataacaaa tggaaaatac aagagtgtaa tgcacagagt gatagcacag     720

gaggatggaa acagaatgtc gatagcatca ttctacaatc caggaaacga cgccgtaatc     780

tatccggcgc cggcgctggt cgagggagag caggagaaaa ccaaacttta cccaaaattt     840

gtgttcgatg attacatgaa gctgtatatg gggcttaagt ttcaagcaaa agagccaagg     900

tttgaggcca tgaaagccat ggagtccacc aatatcaata tgggtccaat tgcaactgtc     960

tga                                                                   963


<210> 4
<211> 113
<212> PRT
<213> Cucumis sativus


<220> 
<223> Mutant ACO2

<400> 4
Met Glu Met Asp Phe Pro Val Ile Asn Met Asn Asn Leu Asn Gly Glu 
1               5                   10                  15      
Ser Arg Val Ser Val Leu Asn Gln Ile Asn Asp Ala Cys Glu Asn Trp 
            20                  25                  30          
Gly Phe Phe Glu Leu Val Asn His Gly Ile Pro His Glu Leu Met Asp 
        35                  40                  45              
Lys Val Glu Lys Met Thr Lys Glu His Tyr Arg Lys Cys Met Glu Gln 
    50                  55                  60                  
Arg Phe Lys Glu Met Val Ala Ser Lys Gly Leu Asp Ser Val Glu Thr 
65                  70                  75                  80  
Glu Ile Asn Asp Thr Asp Trp Glu Ser Thr Phe Phe Tyr Ala Ile Phe 
                85                  90                  95      
Gln Phe Gln Thr Cys Gln Lys Leu Val Ile Trp Met Arg Ser Thr Arg 
            100                 105                 110         
Arg 
    

<210> 5
<211> 154
<212> DNA
<213> Cucumis sativus


<220> 
<223> Wildtype marker CS05484

<400> 5
aatggtagct tccaaagggt tggattcagt ggaaactgag atcaacgaca ctgattggga     60

aagcactttt tttttacgcc atcttccagt ttcaaacatg tcagaaattg gtgatctgga    120

tgaggagtac aagaaggtga tgaaggaatt tgca                                154


<210> 6
<211> 154
<212> DNA
<213> Cucumis sativus


<220> 
<223> Mutant marker CS05484

<220> 
<221> misc_feature
<222> 75
<223> /note="n = a or c or t or g"

<400> 6
aatggtagct tccaaagggt tggattcagt ggaaactgag atcaacgaca ctgattggga     60

aagcactttt ttttnacgcc atcttccagt ttcaaacatg tcagaaattg gtgatctgga    120

tgaggagtac aagaaggtga tgaaggaatt tgca                                154


