                               SEQUENCE LISTING

<110> THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
 
<120> CHIMERIC ANTIGEN RECEPTOR T CELLS DERIVED FROM IMMUNOENGINEERED 
      PLURIPOTENT STEM CELLS

<130> RUC006W

<140> PCT/US2019/042123
<141> 2019-07-17

<150> 62/698,941
<151> 2018-07-17

<160> 6     

<170> PatentIn version 3.5

<210> 1
<211> 119
<212> PRT
<213> Homo sapiens

<400> 1
Met Ser Arg Ser Val Ala Leu Ala Val Leu Ala Leu Leu Ser Leu Ser 
1               5                   10                  15      


Gly Leu Glu Ala Ile Gln Arg Thr Pro Lys Ile Gln Val Tyr Ser Arg 
            20                  25                  30          


His Pro Ala Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys Tyr Val Ser 
        35                  40                  45              


Gly Phe His Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu 
    50                  55                  60                  


Arg Ile Glu Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp 
65                  70                  75                  80  


Ser Phe Tyr Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp 
                85                  90                  95      


Glu Tyr Ala Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile 
            100                 105                 110         


Val Lys Trp Asp Arg Asp Ile 
        115                 


<210> 2
<211> 160
<212> PRT
<213> Homo sapiens

<400> 2
Met Arg Cys Leu Ala Pro Arg Pro Ala Gly Ser Tyr Leu Ser Glu Pro 
1               5                   10                  15      


Gln Gly Ser Ser Gln Cys Ala Thr Met Glu Leu Gly Pro Leu Glu Gly 
            20                  25                  30          


Gly Tyr Leu Glu Leu Leu Asn Ser Asp Ala Asp Pro Leu Cys Leu Tyr 
        35                  40                  45              


His Phe Tyr Asp Gln Met Asp Leu Ala Gly Glu Glu Glu Ile Glu Leu 
    50                  55                  60                  


Tyr Ser Glu Pro Asp Thr Asp Thr Ile Asn Cys Asp Gln Phe Ser Arg 
65                  70                  75                  80  


Leu Leu Cys Asp Met Glu Gly Asp Glu Glu Thr Arg Glu Ala Tyr Ala 
                85                  90                  95      


Asn Ile Ala Glu Leu Asp Gln Tyr Val Phe Gln Asp Ser Gln Leu Glu 
            100                 105                 110         


Gly Leu Ser Lys Asp Ile Phe Lys His Ile Gly Pro Asp Glu Val Ile 
        115                 120                 125             


Gly Glu Ser Met Glu Met Pro Ala Glu Val Gly Gln Lys Ser Gln Lys 
    130                 135                 140                 


Arg Pro Phe Pro Glu Glu Leu Pro Ala Asp Leu Lys His Trp Lys Pro 
145                 150                 155                 160 


<210> 3
<211> 293
<212> PRT
<213> Homo sapiens

<400> 3
Met Trp Pro Leu Val Ala Ala Leu Leu Leu Gly Ser Ala Cys Cys Gly 
1               5                   10                  15      


Ser Ala Gln Leu Leu Phe Asn Lys Thr Lys Ser Val Glu Phe Thr Phe 
            20                  25                  30          


Cys Asn Asp Thr Val Val Ile Pro Cys Phe Val Thr Asn Met Glu Ala 
        35                  40                  45              


Gln Asn Thr Thr Glu Val Tyr Val Lys Trp Lys Phe Lys Gly Arg Asp 
    50                  55                  60                  


Ile Tyr Thr Phe Asp Gly Ala Leu Asn Lys Ser Thr Val Pro Thr Asp 
65                  70                  75                  80  


Phe Ser Ser Ala Lys Ile Glu Val Ser Gln Leu Leu Lys Gly Asp Ala 
                85                  90                  95      


Ser Leu Lys Met Asp Lys Ser Asp Ala Val Ser His Thr Gly Asn Tyr 
            100                 105                 110         


Thr Cys Glu Val Thr Glu Leu Thr Arg Glu Gly Glu Thr Ile Ile Glu 
        115                 120                 125             


Leu Lys Tyr Arg Val Val Ser Trp Phe Ser Pro Asn Glu Asn Ile Leu 
    130                 135                 140                 


Ile Val Ile Phe Pro Ile Phe Ala Ile Leu Leu Phe Trp Gly Gln Phe 
145                 150                 155                 160 


Gly Ile Lys Thr Leu Lys Tyr Arg Ser Gly Gly Met Asp Glu Lys Thr 
                165                 170                 175     


Ile Ala Leu Leu Val Ala Gly Leu Val Ile Thr Val Ile Val Ile Val 
            180                 185                 190         


Gly Ala Ile Leu Phe Val Pro Gly Glu Tyr Ser Leu Lys Asn Ala Thr 
        195                 200                 205             


Gly Leu Gly Leu Ile Val Thr Ser Thr Gly Ile Leu Ile Leu Leu His 
    210                 215                 220                 


Tyr Tyr Val Phe Ser Thr Ala Ile Gly Leu Thr Ser Phe Val Ile Ala 
225                 230                 235                 240 


Ile Leu Val Ile Gln Val Ile Ala Tyr Ile Leu Ala Val Val Gly Leu 
                245                 250                 255     


Ser Leu Cys Ile Ala Ala Cys Ile Pro Met His Gly Pro Leu Leu Ile 
            260                 265                 270         


Ser Gly Leu Ser Ile Leu Ala Leu Ala Gln Leu Leu Gly Leu Val Tyr 
        275                 280                 285             


Met Lys Phe Val Glu 
    290             


<210> 4
<211> 376
<212> PRT
<213> Herpes simplex virus

<400> 4
Met Ala Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala 
1               5                   10                  15      


Ala Arg Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg 
            20                  25                  30          


Arg Gln Gln Glu Ala Thr Glu Val Arg Leu Glu Gln Lys Met Pro Thr 
        35                  40                  45              


Leu Leu Arg Val Tyr Ile Asp Gly Pro His Gly Met Gly Lys Thr Thr 
    50                  55                  60                  


Thr Thr Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr 
65                  70                  75                  80  


Val Pro Glu Pro Met Thr Tyr Trp Gln Val Leu Gly Ala Ser Glu Thr 
                85                  90                  95      


Ile Ala Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile 
            100                 105                 110         


Ser Ala Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met 
        115                 120                 125             


Gly Met Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Val Gly 
    130                 135                 140                 


Gly Glu Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Ile 
145                 150                 155                 160 


Phe Asp Arg His Pro Ile Ala Ala Leu Leu Cys Tyr Pro Ala Ala Arg 
                165                 170                 175     


Tyr Leu Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala 
            180                 185                 190         


Leu Ile Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu 
        195                 200                 205             


Pro Glu Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly 
    210                 215                 220                 


Glu Arg Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly 
225                 230                 235                 240 


Leu Leu Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Trp 
                245                 250                 255     


Glu Asp Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala 
            260                 265                 270         


Glu Pro Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu 
        275                 280                 285             


Phe Thr Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu 
    290                 295                 300                 


Tyr Asn Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg 
305                 310                 315                 320 


Pro Met His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys 
                325                 330                 335     


Arg Asp Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val 
            340                 345                 350         


Thr Thr Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe 
        355                 360                 365             


Ala Arg Glu Met Gly Glu Ala Asn 
    370                 375     


<210> 5
<211> 427
<212> PRT
<213> Escherichia coli

<400> 5
Met Ser Asn Asn Ala Leu Gln Thr Ile Ile Asn Ala Arg Leu Pro Gly 
1               5                   10                  15      


Glu Glu Gly Leu Trp Gln Ile His Leu Gln Asp Gly Lys Ile Ser Ala 
            20                  25                  30          


Ile Asp Ala Gln Ser Gly Val Met Pro Ile Thr Glu Asn Ser Leu Asp 
        35                  40                  45              


Ala Glu Gln Gly Leu Val Ile Pro Pro Phe Val Glu Pro His Ile His 
    50                  55                  60                  


Leu Asp Thr Thr Gln Thr Ala Gly Gln Pro Asn Trp Asn Gln Ser Gly 
65                  70                  75                  80  


Thr Leu Phe Glu Gly Ile Glu Arg Trp Ala Glu Arg Lys Ala Leu Leu 
                85                  90                  95      


Thr His Asp Asp Val Lys Gln Arg Ala Trp Gln Thr Leu Lys Trp Gln 
            100                 105                 110         


Ile Ala Asn Gly Ile Gln His Val Arg Thr His Val Asp Val Ser Asp 
        115                 120                 125             


Ala Thr Leu Thr Ala Leu Lys Ala Met Leu Glu Val Lys Gln Glu Val 
    130                 135                 140                 


Ala Pro Trp Ile Asp Leu Gln Ile Val Ala Phe Pro Gln Glu Gly Ile 
145                 150                 155                 160 


Leu Ser Tyr Pro Asn Gly Glu Ala Leu Leu Glu Glu Ala Leu Arg Leu 
                165                 170                 175     


Gly Ala Asp Val Val Gly Ala Ile Pro His Phe Glu Phe Thr Arg Glu 
            180                 185                 190         


Tyr Gly Val Glu Ser Leu His Lys Thr Phe Ala Leu Ala Gln Lys Tyr 
        195                 200                 205             


Asp Arg Leu Ile Asp Val His Cys Asp Glu Ile Asp Asp Glu Gln Ser 
    210                 215                 220                 


Arg Phe Val Glu Thr Val Ala Ala Leu Ala His His Glu Gly Met Gly 
225                 230                 235                 240 


Ala Arg Val Thr Ala Ser His Thr Thr Ala Met His Ser Tyr Asn Gly 
                245                 250                 255     


Ala Tyr Thr Ser Arg Leu Phe Arg Leu Leu Lys Met Ser Gly Ile Asn 
            260                 265                 270         


Phe Val Ala Asn Pro Leu Val Asn Ile His Leu Gln Gly Arg Phe Asp 
        275                 280                 285             


Thr Tyr Pro Lys Arg Arg Gly Ile Thr Arg Val Lys Glu Met Leu Glu 
    290                 295                 300                 


Ser Gly Ile Asn Val Cys Phe Gly His Asp Asp Val Phe Asp Pro Trp 
305                 310                 315                 320 


Tyr Pro Leu Gly Thr Ala Asn Met Leu Gln Val Leu His Met Gly Leu 
                325                 330                 335     


His Val Cys Gln Leu Met Gly Tyr Gly Gln Ile Asn Asp Gly Leu Asn 
            340                 345                 350         


Leu Ile Thr His His Ser Ala Arg Thr Leu Asn Leu Gln Asp Tyr Gly 
        355                 360                 365             


Ile Ala Ala Gly Asn Ser Ala Asn Leu Ile Ile Leu Pro Ala Glu Asn 
    370                 375                 380                 


Gly Phe Asp Ala Leu Arg Arg Gln Val Pro Val Arg Tyr Ser Val Arg 
385                 390                 395                 400 


Gly Gly Lys Val Ile Ala Ser Thr Gln Pro Ala Gln Thr Thr Val Tyr 
                405                 410                 415     


Leu Glu Gln Pro Glu Ala Ile Asp Tyr Lys Arg 
            420                 425         


<210> 6
<211> 282
<212> PRT
<213> Homo sapiens

<400> 6
Gly Phe Gly Asp Val Gly Ala Leu Glu Ser Leu Arg Gly Asn Ala Asp 
1               5                   10                  15      


Leu Ala Tyr Ile Leu Ser Met Glu Pro Cys Gly His Cys Leu Ile Ile 
            20                  25                  30          


Asn Asn Val Asn Phe Cys Arg Glu Ser Gly Leu Arg Thr Arg Thr Gly 
        35                  40                  45              


Ser Asn Ile Asp Cys Glu Lys Leu Arg Arg Arg Phe Ser Ser Leu His 
    50                  55                  60                  


Phe Met Val Glu Val Lys Gly Asp Leu Thr Ala Lys Lys Met Val Leu 
65                  70                  75                  80  


Ala Leu Leu Glu Leu Ala Gln Gln Asp His Gly Ala Leu Asp Cys Cys 
                85                  90                  95      


Val Val Val Ile Leu Ser His Gly Cys Gln Ala Ser His Leu Gln Phe 
            100                 105                 110         


Pro Gly Ala Val Tyr Gly Thr Asp Gly Cys Pro Val Ser Val Glu Lys 
        115                 120                 125             


Ile Val Asn Ile Phe Asn Gly Thr Ser Cys Pro Ser Leu Gly Gly Lys 
    130                 135                 140                 


Pro Lys Leu Phe Phe Ile Gln Ala Cys Gly Gly Glu Gln Lys Asp His 
145                 150                 155                 160 


Gly Phe Glu Val Ala Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser 
                165                 170                 175     


Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe 
            180                 185                 190         


Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe 
        195                 200                 205             


Val Ser Tyr Ser Thr Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys 
    210                 215                 220                 


Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp 
225                 230                 235                 240 


Ala His Ser Glu Asp Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala 
                245                 250                 255     


Val Ser Val Lys Gly Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe 
            260                 265                 270         


Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser 
        275                 280         


