                         SEQUENCE LISTING

<110>  Fred Hutchinson Cancer Research Center
       Orozco, Johnnie J.
 
<120>  Bispecific Affinity Reagent and Related Methods for Pretargeted 
       Radioimmunotherapy Against CD45+ Cells

<130>  FHTM-1-69222

<150>  US 62/698643
<151>  2018-07-16

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  4315
<212>  DNA
<213>  Homo sapiens

<400>  1
ggaaattgtt cctcgtctga taagacaaca gtggagaaag gacgcatgct gtttcttagg       60

gacacggctg gcttccagat atgaccatgt atttgtggct taaactcttg gcatttggct      120

ttgcctttct ggacacagaa gtatttgtga cagggcaaag cccaacacct tcccccactg      180

gattgactac agcaaagatg cccagtgttc cactttcaag tgacccctta cctactcaca      240

ccactgcatt ctcacccgca agcacctttg aaagagaaaa tgacttctca gagaccacaa      300

cttctcttag tccagacaat acttccaccc aagtatcccc ggactctttg gataatgcta      360

gtgcttttaa taccacaggt gtttcatcag tacagacgcc tcaccttccc acgcacgcag      420

actcgcagac gccctctgct ggaactgaca cgcagacatt cagcggctcc gccgccaatg      480

caaaactcaa ccctacccca ggcagcaatg ctatctcaga tgtcccagga gagaggagta      540

cagccagcac ctttcctaca gacccagttt ccccattgac aaccaccctc agccttgcac      600

accacagctc tgctgcctta cctgcacgca cctccaacac caccatcaca gcgaacacct      660

cagatgccta ccttaatgcc tctgaaacaa ccactctgag cccttctgga agcgctgtca      720

tttcaaccac aacaatagct actactccat ctaagccaac atgtgatgaa aaatatgcaa      780

acatcactgt ggattactta tataacaagg aaactaaatt atttacagca aagctaaatg      840

ttaatgagaa tgtggaatgt ggaaacaata cttgcacaaa caatgaggtg cataacctta      900

cagaatgtaa aaatgcgtct gtttccatat ctcataattc atgtactgct cctgataaga      960

cattaatatt agatgtgcca ccaggggttg aaaagtttca gttacatgat tgtacacaag     1020

ttgaaaaagc agatactact atttgtttaa aatggaaaaa tattgaaacc tttacttgtg     1080

atacacagaa tattacctac agatttcagt gtggtaatat gatatttgat aataaagaaa     1140

ttaaattaga aaaccttgaa cccgaacatg agtataagtg tgactcagaa atactctata     1200

ataaccacaa gtttactaac gcaagtaaaa ttattaaaac agattttggg agtccaggag     1260

agcctcagat tattttttgt agaagtgaag ctgcacatca aggagtaatt acctggaatc     1320

cccctcaaag atcatttcat aattttaccc tctgttatat aaaagagaca gaaaaagatt     1380

gcctcaatct ggataaaaac ctgatcaaat atgatttgca aaatttaaaa ccttatacga     1440

aatatgtttt atcattacat gcctacatca ttgcaaaagt gcaacgtaat ggaagtgctg     1500

caatgtgtca tttcacaact aaaagtgctc ctccaagcca ggtctggaac atgactgtct     1560

ccatgacatc agataatagt atgcatgtca agtgtaggcc tcccagggac cgtaatggcc     1620

cccatgaacg ttaccatttg gaagttgaag ctggaaatac tctggttaga aatgagtcgc     1680

ataagaattg cgatttccgt gtaaaagatc ttcaatattc aacagactac acttttaagg     1740

cctattttca caatggagac tatcctggag aaccctttat tttacatcat tcaacatctt     1800

ataattctaa ggcactgata gcatttctgg catttctgat tattgtgaca tcaatagccc     1860

tgcttgttgt tctctacaaa atctatgatc tacataagaa aagatcctgc aatttagatg     1920

aacagcagga gcttgttgaa agggatgatg aaaaacaact gatgaatgtg gagccaatcc     1980

atgcagatat tttgttggaa acttataaga ggaagattgc tgatgaagga agaccttttc     2040

tggctgaatt tcagagcatc ccgcgggtgt tcagcaagtt tcctataaag gaagctcgaa     2100

agccctttaa ccagaataaa aaccgttatg ttgacattct tccttatgat tataaccgtg     2160

ttgaactctc tgagataaac ggagatgcag ggtcaaacta cataaatgcc agctatattg     2220

atggtttcaa agaacccagg aaatacattg ctgcacaagg tcccagggat gaaactgttg     2280

atgatttctg gaggatgatt tgggaacaga aagccacagt tattgtcatg gtcactcgat     2340

gtgaagaagg aaacaggaac aagtgtgcag aatactggcc gtcaatggaa gagggcactc     2400

gggcttttgg agatgttgtt gtaaagatca accagcacaa aagatgtcca gattacatca     2460

ttcagaaatt gaacattgta aataaaaaag aaaaagcaac tggaagagag gtgactcaca     2520

ttcagttcac cagctggcca gaccacgggg tgcctgagga tcctcacttg ctcctcaaac     2580

tgagaaggag agtgaatgcc ttcagcaatt tcttcagtgg tcccattgtg gtgcactgca     2640

gtgctggtgt tgggcgcaca ggaacctata tcggaattga tgccatgcta gaaggcttgg     2700

aagccgagaa caaagtggat gtttatggtt atgttgtcaa gctaaggcga cagagatgcc     2760

tgatggttca agtagaggcc cagtacatct tgatccatca ggctttggtg gaatacaatc     2820

agtttggaga aacagaagtg aatttgtctg aattacatcc atatctacat aacatgaaga     2880

aaagggatcc acccagtgag ccgtctccac tagaggctga attccagaga cttccttcat     2940

ataggagctg gaggacacag cacattggaa atcaagaaga aaataaaagt aaaaacagga     3000

attctaatgt catcccatat gactataaca gagtgccact taaacatgag ctggaaatga     3060

gtaaagagag tgagcatgat tcagatgaat cctctgatga tgacagtgat tcagaggaac     3120

caagcaaata catcaatgca tcttttataa tgagctactg gaaacctgaa gtgatgattg     3180

ctgctcaggg accactgaag gagaccattg gtgacttttg gcagatgatc ttccaaagaa     3240

aagtcaaagt tattgttatg ctgacagaac tgaaacatgg agaccaggaa atctgtgctc     3300

agtactgggg agaaggaaag caaacatatg gagatattga agttgacctg aaagacacag     3360

acaaatcttc aacttatacc cttcgtgtct ttgaactgag acattccaag aggaaagact     3420

ctcgaactgt gtaccagtac caatatacaa actggagtgt ggagcagctt cctgcagaac     3480

ccaaggaatt aatctctatg attcaggtcg tcaaacaaaa acttccccag aagaattcct     3540

ctgaagggaa caagcatcac aagagtacac ctctactcat tcactgcagg gatggatctc     3600

agcaaacggg aatattttgt gctttgttaa atctcttaga aagtgcggaa acagaagagg     3660

tagtggatat ttttcaagtg gtaaaagctc tacgcaaagc taggctaggc atggtttcca     3720

cattcgagca atatcaattc ctatatgacg tcattgccag cacctaccct gctcagaatg     3780

gacaagtaaa gaaaaacaac catcaagaag ataaaattga atttgataat gaagtggaca     3840

aagtaaagca ggatgctaat tgtgttaatc cacttggtgc cccagaaaag ctccctgaag     3900

caaaggaaca ggctgaaggt tctgaaccca cgagtggcac tgaggggcca gaacattctg     3960

tcaatggtcc tgcaagtcca gctttaaatc aaggttcata ggaaaagaca taaatgagga     4020

aactccaaac ctcctgttag ctgttatttc tatttttgta gaagtaggaa gtgaaaatag     4080

gtatacagtg gattaattaa atgcagcgaa ccaatatttg tagaagggtt atattttact     4140

actgtggaaa aatatttaag atagttttgc cagaacagtt tgtacagacg tatgcttatt     4200

ttaaaatttt atctcttatt cagtaaaaaa caacttcttt gtaatcgtta tgagtgtata     4260

tgtatgtgtg tatgggtgtg tgtttgtgtg agagacagag aaagagagag aattc          4315


<210>  2
<211>  1306
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Thr Met Tyr Leu Trp Leu Lys Leu Leu Ala Phe Gly Phe Ala Phe 
1               5                   10                  15      


Leu Asp Thr Glu Val Phe Val Thr Gly Gln Ser Pro Thr Pro Ser Pro 
            20                  25                  30          


Thr Gly Leu Thr Thr Ala Lys Met Pro Ser Val Pro Leu Ser Ser Asp 
        35                  40                  45              


Pro Leu Pro Thr His Thr Thr Ala Phe Ser Pro Ala Ser Thr Phe Glu 
    50                  55                  60                  


Arg Glu Asn Asp Phe Ser Glu Thr Thr Thr Ser Leu Ser Pro Asp Asn 
65                  70                  75                  80  


Thr Ser Thr Gln Val Ser Pro Asp Ser Leu Asp Asn Ala Ser Ala Phe 
                85                  90                  95      


Asn Thr Thr Gly Val Ser Ser Val Gln Thr Pro His Leu Pro Thr His 
            100                 105                 110         


Ala Asp Ser Gln Thr Pro Ser Ala Gly Thr Asp Thr Gln Thr Phe Ser 
        115                 120                 125             


Gly Ser Ala Ala Asn Ala Lys Leu Asn Pro Thr Pro Gly Ser Asn Ala 
    130                 135                 140                 


Ile Ser Asp Val Pro Gly Glu Arg Ser Thr Ala Ser Thr Phe Pro Thr 
145                 150                 155                 160 


Asp Pro Val Ser Pro Leu Thr Thr Thr Leu Ser Leu Ala His His Ser 
                165                 170                 175     


Ser Ala Ala Leu Pro Ala Arg Thr Ser Asn Thr Thr Ile Thr Ala Asn 
            180                 185                 190         


Thr Ser Asp Ala Tyr Leu Asn Ala Ser Glu Thr Thr Thr Leu Ser Pro 
        195                 200                 205             


Ser Gly Ser Ala Val Ile Ser Thr Thr Thr Ile Ala Thr Thr Pro Ser 
    210                 215                 220                 


Lys Pro Thr Cys Asp Glu Lys Tyr Ala Asn Ile Thr Val Asp Tyr Leu 
225                 230                 235                 240 


Tyr Asn Lys Glu Thr Lys Leu Phe Thr Ala Lys Leu Asn Val Asn Glu 
                245                 250                 255     


Asn Val Glu Cys Gly Asn Asn Thr Cys Thr Asn Asn Glu Val His Asn 
            260                 265                 270         


Leu Thr Glu Cys Lys Asn Ala Ser Val Ser Ile Ser His Asn Ser Cys 
        275                 280                 285             


Thr Ala Pro Asp Lys Thr Leu Ile Leu Asp Val Pro Pro Gly Val Glu 
    290                 295                 300                 


Lys Phe Gln Leu His Asp Cys Thr Gln Val Glu Lys Ala Asp Thr Thr 
305                 310                 315                 320 


Ile Cys Leu Lys Trp Lys Asn Ile Glu Thr Phe Thr Cys Asp Thr Gln 
                325                 330                 335     


Asn Ile Thr Tyr Arg Phe Gln Cys Gly Asn Met Ile Phe Asp Asn Lys 
            340                 345                 350         


Glu Ile Lys Leu Glu Asn Leu Glu Pro Glu His Glu Tyr Lys Cys Asp 
        355                 360                 365             


Ser Glu Ile Leu Tyr Asn Asn His Lys Phe Thr Asn Ala Ser Lys Ile 
    370                 375                 380                 


Ile Lys Thr Asp Phe Gly Ser Pro Gly Glu Pro Gln Ile Ile Phe Cys 
385                 390                 395                 400 


Arg Ser Glu Ala Ala His Gln Gly Val Ile Thr Trp Asn Pro Pro Gln 
                405                 410                 415     


Arg Ser Phe His Asn Phe Thr Leu Cys Tyr Ile Lys Glu Thr Glu Lys 
            420                 425                 430         


Asp Cys Leu Asn Leu Asp Lys Asn Leu Ile Lys Tyr Asp Leu Gln Asn 
        435                 440                 445             


Leu Lys Pro Tyr Thr Lys Tyr Val Leu Ser Leu His Ala Tyr Ile Ile 
    450                 455                 460                 


Ala Lys Val Gln Arg Asn Gly Ser Ala Ala Met Cys His Phe Thr Thr 
465                 470                 475                 480 


Lys Ser Ala Pro Pro Ser Gln Val Trp Asn Met Thr Val Ser Met Thr 
                485                 490                 495     


Ser Asp Asn Ser Met His Val Lys Cys Arg Pro Pro Arg Asp Arg Asn 
            500                 505                 510         


Gly Pro His Glu Arg Tyr His Leu Glu Val Glu Ala Gly Asn Thr Leu 
        515                 520                 525             


Val Arg Asn Glu Ser His Lys Asn Cys Asp Phe Arg Val Lys Asp Leu 
    530                 535                 540                 


Gln Tyr Ser Thr Asp Tyr Thr Phe Lys Ala Tyr Phe His Asn Gly Asp 
545                 550                 555                 560 


Tyr Pro Gly Glu Pro Phe Ile Leu His His Ser Thr Ser Tyr Asn Ser 
                565                 570                 575     


Lys Ala Leu Ile Ala Phe Leu Ala Phe Leu Ile Ile Val Thr Ser Ile 
            580                 585                 590         


Ala Leu Leu Val Val Leu Tyr Lys Ile Tyr Asp Leu His Lys Lys Arg 
        595                 600                 605             


Ser Cys Asn Leu Asp Glu Gln Gln Glu Leu Val Glu Arg Asp Asp Glu 
    610                 615                 620                 


Lys Gln Leu Met Asn Val Glu Pro Ile His Ala Asp Ile Leu Leu Glu 
625                 630                 635                 640 


Thr Tyr Lys Arg Lys Ile Ala Asp Glu Gly Arg Leu Phe Leu Ala Glu 
                645                 650                 655     


Phe Gln Ser Ile Pro Arg Val Phe Ser Lys Phe Pro Ile Lys Glu Ala 
            660                 665                 670         


Arg Lys Pro Phe Asn Gln Asn Lys Asn Arg Tyr Val Asp Ile Leu Pro 
        675                 680                 685             


Tyr Asp Tyr Asn Arg Val Glu Leu Ser Glu Ile Asn Gly Asp Ala Gly 
    690                 695                 700                 


Ser Asn Tyr Ile Asn Ala Ser Tyr Ile Asp Gly Phe Lys Glu Pro Arg 
705                 710                 715                 720 


Lys Tyr Ile Ala Ala Gln Gly Pro Arg Asp Glu Thr Val Asp Asp Phe 
                725                 730                 735     


Trp Arg Met Ile Trp Glu Gln Lys Ala Thr Val Ile Val Met Val Thr 
            740                 745                 750         


Arg Cys Glu Glu Gly Asn Arg Asn Lys Cys Ala Glu Tyr Trp Pro Ser 
        755                 760                 765             


Met Glu Glu Gly Thr Arg Ala Phe Gly Asp Val Val Val Lys Ile Asn 
    770                 775                 780                 


Gln His Lys Arg Cys Pro Asp Tyr Ile Ile Gln Lys Leu Asn Ile Val 
785                 790                 795                 800 


Asn Lys Lys Glu Lys Ala Thr Gly Arg Glu Val Thr His Ile Gln Phe 
                805                 810                 815     


Thr Ser Trp Pro Asp His Gly Val Pro Glu Asp Pro His Leu Leu Leu 
            820                 825                 830         


Lys Leu Arg Arg Arg Val Asn Ala Phe Ser Asn Phe Phe Ser Gly Pro 
        835                 840                 845             


Ile Val Val His Cys Ser Ala Gly Val Gly Arg Thr Gly Thr Tyr Ile 
    850                 855                 860                 


Gly Ile Asp Ala Met Leu Glu Gly Leu Glu Ala Glu Asn Lys Val Asp 
865                 870                 875                 880 


Val Tyr Gly Tyr Val Val Lys Leu Arg Arg Gln Arg Cys Leu Met Val 
                885                 890                 895     


Gln Val Glu Ala Gln Tyr Ile Leu Ile His Gln Ala Leu Val Glu Tyr 
            900                 905                 910         


Asn Gln Phe Gly Glu Thr Glu Val Asn Leu Ser Glu Leu His Pro Tyr 
        915                 920                 925             


Leu His Asn Met Lys Lys Arg Asp Pro Pro Ser Glu Pro Ser Pro Leu 
    930                 935                 940                 


Glu Ala Glu Phe Gln Arg Leu Pro Ser Tyr Arg Ser Trp Arg Thr Gln 
945                 950                 955                 960 


His Ile Gly Asn Gln Glu Glu Asn Lys Ser Lys Asn Arg Asn Ser Asn 
                965                 970                 975     


Val Ile Pro Tyr Asp Tyr Asn Arg Val Pro Leu Lys His Glu Leu Glu 
            980                 985                 990         


Met Ser Lys Glu Ser Glu His Asp  Ser Asp Glu Ser Ser  Asp Asp Asp 
        995                 1000                 1005             


Ser Asp  Ser Glu Glu Pro Ser  Lys Tyr Ile Asn Ala  Ser Phe Ile 
    1010                 1015                 1020             


Met Ser  Tyr Trp Lys Pro Glu  Val Met Ile Ala Ala  Gln Gly Pro 
    1025                 1030                 1035             


Leu Lys  Glu Thr Ile Gly Asp  Phe Trp Gln Met Ile  Phe Gln Arg 
    1040                 1045                 1050             


Lys Val  Lys Val Ile Val Met  Leu Thr Glu Leu Lys  His Gly Asp 
    1055                 1060                 1065             


Gln Glu  Ile Cys Ala Gln Tyr  Trp Gly Glu Gly Lys  Gln Thr Tyr 
    1070                 1075                 1080             


Gly Asp  Ile Glu Val Asp Leu  Lys Asp Thr Asp Lys  Ser Ser Thr 
    1085                 1090                 1095             


Tyr Thr  Leu Arg Val Phe Glu  Leu Arg His Ser Lys  Arg Lys Asp 
    1100                 1105                 1110             


Ser Arg  Thr Val Tyr Gln Tyr  Gln Tyr Thr Asn Trp  Ser Val Glu 
    1115                 1120                 1125             


Gln Leu  Pro Ala Glu Pro Lys  Glu Leu Ile Ser Met  Ile Gln Val 
    1130                 1135                 1140             


Val Lys  Gln Lys Leu Pro Gln  Lys Asn Ser Ser Glu  Gly Asn Lys 
    1145                 1150                 1155             


His His  Lys Ser Thr Pro Leu  Leu Ile His Cys Arg  Asp Gly Ser 
    1160                 1165                 1170             


Gln Gln  Thr Gly Ile Phe Cys  Ala Leu Leu Asn Leu  Leu Glu Ser 
    1175                 1180                 1185             


Ala Glu  Thr Glu Glu Val Val  Asp Ile Phe Gln Val  Val Lys Ala 
    1190                 1195                 1200             


Leu Arg  Lys Ala Arg Pro Gly  Met Val Ser Thr Phe  Glu Gln Tyr 
    1205                 1210                 1215             


Gln Phe  Leu Tyr Asp Val Ile  Ala Ser Thr Tyr Pro  Ala Gln Asn 
    1220                 1225                 1230             


Gly Gln  Val Lys Lys Asn Asn  His Gln Glu Asp Lys  Ile Glu Phe 
    1235                 1240                 1245             


Asp Asn  Glu Val Asp Lys Val  Lys Gln Asp Ala Asn  Cys Val Asn 
    1250                 1255                 1260             


Pro Leu  Gly Ala Pro Glu Lys  Leu Pro Glu Ala Lys  Glu Gln Ala 
    1265                 1270                 1275             


Glu Gly  Ser Glu Pro Thr Ser  Gly Thr Glu Gly Pro  Glu His Ser 
    1280                 1285                 1290             


Val Asn  Gly Pro Ala Ser Pro  Ala Leu Asn Gln Gly  Ser 
    1295                 1300                 1305     


