                               SEQUENCE LISTING

<110> THE CHILDREN'S MEDICAL CENTER CORPORATION
 
<120> METHOD FOR TREATING CANCER

<130> 701039-093110WOPT

<140> PCT/US2019/041555
<141> 2019-07-12

<150> 62/839,912
<151> 2019-04-29

<150> 62/751,129
<151> 2018-10-26

<150> 62/697,053
<151> 2018-07-12

<160> 25    

<170> PatentIn version 3.5

<210> 1
<211> 1461
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      GSK3-alpha sequence

<400> 1
ctcggcgcca tgagcggcgg cgggccttcg ggaggcggcc ctgggggctc gggcagggcg       60

cggactagct cgttcgcgga gcccggcggc ggaggcggag gaggcggcgg cggccccgga      120

ggctcggcct ccggcccagg cggcaccggc ggcggaaagg catctgtcgg ggccatgggt      180

gggggcgtcg gggcctcgag ctccgggggt ggacccggcg gcagcggcgg aggaggcagc      240

ggaggccccg gcgcaggcac tagcttcccg ccgcccgggg tgaagctggg ccgtgacagc      300

gggaaggtga ccacagtcgt agccactcta ggccaaggcc cagagcgctc ccaagaagtg      360

gcttacacgg acatcaaagt gattggcaat ggctcatttg gggtcgtgta ccaggcacgg      420

ctggcagaga ccagggaact agtcgccatc aagaaggttc tccaggacaa gaggttcaag      480

aaccgagagc tgcagatcat gcgtaagctg gaccactgca atattgtgag gctgagatac      540

tttttctact ccagtggcga gaagaaagac gagctttacc taaatctggt gctggaatat      600

gtgcccgaga cagtgtaccg ggtggcccgc cacttcacca aggccaagtt gaccatccct      660

atcctctatg tcaaggtgta catgtaccag ctcttccgca gcttggccta catccactcc      720

cagggcgtgt gtcaccgcga catcaagccc cagaacctgc tggtggaccc tgacactgct      780

gtcctcaagc tctgcgattt tggcagtgca aagcagttgg tccgagggga gcccaatgtc      840

tcctacatct gttctcgcta ctaccgggcc ccagagctca tctttggagc cactgattac      900

acctcatcca tcgatgtttg gtcagctggc tgtgtactgg cagagctcct cttgggccag      960

cccatcttcc ctggggacag tggggtggac cagctggtgg agatcatcaa ggtgctggga     1020

acaccaaccc gggaacaaat ccgagagatg aaccccaact acacggagtt caagttccct     1080

cagattaaag ctcacccctg gacaaaggtg ttcaaatctc gaacgccgcc agaggccatc     1140

gcgctctgct ctagcctgct ggagtacacc ccatcctcaa ggctctcccc actagaggcc     1200

tgtgcgcaca gcttctttga tgaactgcga tgtctgggaa cccagctgcc taacaaccgc     1260

ccacttcccc ctctcttcaa cttcagtgct ggtgaactct ccatccaacc gtctctcaac     1320

gccattctca tccctcctca cttgaggtcc ccagcgggca ctaccaccct caccccgtcc     1380

tcacaagctt taactgagac tccgaccagc tcagactggc agtcgaccga tgccacacct     1440

accctcacta actcctcctg a                                               1461


<210> 2
<211> 483
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      GSK3-alpha sequence

<400> 2
Met Ser Gly Gly Gly Pro Ser Gly Gly Gly Pro Gly Gly Ser Gly Arg 
1               5                   10                  15      


Ala Arg Thr Ser Ser Phe Ala Glu Pro Gly Gly Gly Gly Gly Gly Gly 
            20                  25                  30          


Gly Gly Gly Pro Gly Gly Ser Ala Ser Gly Pro Gly Gly Thr Gly Gly 
        35                  40                  45              


Gly Lys Ala Ser Val Gly Ala Met Gly Gly Gly Val Gly Ala Ser Ser 
    50                  55                  60                  


Ser Gly Gly Gly Pro Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Pro 
65                  70                  75                  80  


Gly Ala Gly Thr Ser Phe Pro Pro Pro Gly Val Lys Leu Gly Arg Asp 
                85                  90                  95      


Ser Gly Lys Val Thr Thr Val Val Ala Thr Leu Gly Gln Gly Pro Glu 
            100                 105                 110         


Arg Ser Gln Glu Val Ala Tyr Thr Asp Ile Lys Val Ile Gly Asn Gly 
        115                 120                 125             


Ser Phe Gly Val Val Tyr Gln Ala Arg Leu Ala Glu Thr Arg Glu Leu 
    130                 135                 140                 


Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Phe Lys Asn Arg Glu 
145                 150                 155                 160 


Leu Gln Ile Met Arg Lys Leu Asp His Cys Asn Ile Val Arg Leu Arg 
                165                 170                 175     


Tyr Phe Phe Tyr Ser Ser Gly Glu Lys Lys Asp Glu Leu Tyr Leu Asn 
            180                 185                 190         


Leu Val Leu Glu Tyr Val Pro Glu Thr Val Tyr Arg Val Ala Arg His 
        195                 200                 205             


Phe Thr Lys Ala Lys Leu Thr Ile Pro Ile Leu Tyr Val Lys Val Tyr 
    210                 215                 220                 


Met Tyr Gln Leu Phe Arg Ser Leu Ala Tyr Ile His Ser Gln Gly Val 
225                 230                 235                 240 


Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asp Pro Asp Thr 
                245                 250                 255     


Ala Val Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys Gln Leu Val Arg 
            260                 265                 270         


Gly Glu Pro Asn Val Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg Ala Pro 
        275                 280                 285             


Glu Leu Ile Phe Gly Ala Thr Asp Tyr Thr Ser Ser Ile Asp Val Trp 
    290                 295                 300                 


Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro Ile Phe 
305                 310                 315                 320 


Pro Gly Asp Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys Val Leu 
                325                 330                 335     


Gly Thr Pro Thr Arg Glu Gln Ile Arg Glu Met Asn Pro Asn Tyr Thr 
            340                 345                 350         


Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp Thr Lys Val Phe 
        355                 360                 365             


Lys Ser Arg Thr Pro Pro Glu Ala Ile Ala Leu Cys Ser Ser Leu Leu 
    370                 375                 380                 


Glu Tyr Thr Pro Ser Ser Arg Leu Ser Pro Leu Glu Ala Cys Ala His 
385                 390                 395                 400 


Ser Phe Phe Asp Glu Leu Arg Cys Leu Gly Thr Gln Leu Pro Asn Asn 
                405                 410                 415     


Arg Pro Leu Pro Pro Leu Phe Asn Phe Ser Ala Gly Glu Leu Ser Ile 
            420                 425                 430         


Gln Pro Ser Leu Asn Ala Ile Leu Ile Pro Pro His Leu Arg Ser Pro 
        435                 440                 445             


Ala Gly Thr Thr Thr Leu Thr Pro Ser Ser Gln Ala Leu Thr Glu Thr 
    450                 455                 460                 


Pro Thr Ser Ser Asp Trp Gln Ser Thr Asp Ala Thr Pro Thr Leu Thr 
465                 470                 475                 480 


Asn Ser Ser 
            


<210> 3
<211> 20
<212> DNA
<213> Homo sapiens

<400> 3
ggaagacagc cccgatttac                                                   20


<210> 4
<211> 20
<212> DNA
<213> Homo sapiens

<400> 4
aggatcagat gaacttacgc                                                   20


<210> 5
<211> 20
<212> DNA
<213> Homo sapiens

<400> 5
cagcagtagt tcgatctgcg                                                   20


<210> 6
<211> 20
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      AAVS1 target sequence

<400> 6
agcggctcca attcggaagt                                                   20


<210> 7
<211> 20
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      AAVS1 target sequence

<400> 7
gagaggtgac ccgaatccac                                                   20


<210> 8
<211> 20
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      AAVS1 target sequence

<400> 8
agttcttagg gtaccccacg                                                   20


<210> 9
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
tcttgtggaa aggacgaaac accg                                              24


<210> 10
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
tctactattc tttcccctgc actgt                                             25


<210> 11
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 11
ctggcaccca gcacaatg                                                     18


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 12
gccgatccac acggagtact                                                   20


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
accctccgtg tgaagctaac                                                   20


<210> 14
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
gggcctcctg acgtgtg                                                      17


<210> 15
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
tcgggctgtc cgccgttc                                                     18


<210> 16
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
gctcctccaa gaagcgcagg tg                                                22


<210> 17
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
aaccgctgca gatggctgat gtg                                               23


<210> 18
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
tgcagccatc cttggctacc ct                                                22


<210> 19
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
tccccagcgg gcactacca                                                    19


<210> 20
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
tgagggtagg tgtggcatcg gt                                                22


<210> 21
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
ccaggggata gtggtgtgga tcagt                                             25


<210> 22
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
gaagacccgc actcctgagc tga                                               23


<210> 23
<211> 348
<212> PRT
<213> Escherichia coli

<400> 23
Met Glu Phe Phe Lys Lys Thr Ala Leu Ala Ala Leu Val Met Gly Phe 
1               5                   10                  15      


Ser Gly Ala Ala Leu Ala Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly 
            20                  25                  30          


Gly Thr Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr 
        35                  40                  45              


Val Gly Lys Val Gly Val Glu Asn Leu Val Asn Ala Val Pro Gln Leu 
    50                  55                  60                  


Lys Asp Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn Ile Gly Ser 
65                  70                  75                  80  


Gln Asp Met Asn Asp Asn Val Trp Leu Thr Leu Ala Lys Lys Ile Asn 
                85                  90                  95      


Thr Asp Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His Gly Thr Asp 
            100                 105                 110         


Thr Met Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp 
        115                 120                 125             


Lys Pro Val Val Met Val Gly Ala Met Arg Pro Ser Thr Ser Met Ser 
    130                 135                 140                 


Ala Asp Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr Ala Ala Asp 
145                 150                 155                 160 


Lys Ala Ser Ala Asn Arg Gly Val Leu Val Val Met Asn Asp Thr Val 
                165                 170                 175     


Leu Asp Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp Val Ala Thr 
            180                 185                 190         


Phe Lys Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn Gly Lys 
        195                 200                 205             


Ile Asp Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser Asp Thr Pro 
    210                 215                 220                 


Phe Asp Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly Ile Val Tyr 
225                 230                 235                 240 


Asn Tyr Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu Val Asp Ala 
                245                 250                 255     


Gly Tyr Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr 
            260                 265                 270         


Lys Ser Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Thr Gly Thr Ala 
        275                 280                 285             


Val Val Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr Gln Asp Ala 
    290                 295                 300                 


Glu Val Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn 
305                 310                 315                 320 


Pro Gln Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys 
                325                 330                 335     


Asp Pro Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 345             


<210> 24
<211> 348
<212> PRT
<213> Dickeya chrysanthemi

<400> 24
Met Glu Arg Trp Phe Lys Ser Leu Phe Val Leu Val Leu Phe Phe Val 
1               5                   10                  15      


Phe Thr Ala Ser Ala Ala Asp Lys Leu Pro Asn Ile Val Ile Leu Ala 
            20                  25                  30          


Thr Gly Gly Thr Ile Ala Gly Ser Ala Ala Thr Gly Thr Gln Thr Thr 
        35                  40                  45              


Gly Tyr Lys Ala Gly Ala Leu Gly Val Asp Thr Leu Ile Asn Ala Val 
    50                  55                  60                  


Pro Glu Val Lys Lys Leu Ala Asn Val Lys Gly Glu Gln Phe Ser Asn 
65                  70                  75                  80  


Met Ala Ser Glu Asn Met Thr Gly Asp Val Val Leu Lys Leu Ser Gln 
                85                  90                  95      


Arg Val Asn Glu Leu Leu Ala Arg Asp Asp Val Asp Gly Val Val Ile 
            100                 105                 110         


Thr His Gly Thr Asp Thr Val Glu Glu Ser Ala Tyr Phe Leu His Leu 
        115                 120                 125             


Thr Val Lys Ser Asp Lys Pro Val Val Phe Val Ala Ala Met Arg Pro 
    130                 135                 140                 


Ala Thr Ala Ile Ser Ala Asp Gly Pro Met Asn Leu Leu Glu Ala Val 
145                 150                 155                 160 


Arg Val Ala Gly Asp Lys Gln Ser Arg Gly Arg Gly Val Met Val Val 
                165                 170                 175     


Leu Asn Asp Arg Ile Gly Ser Ala Arg Tyr Ile Thr Lys Thr Asn Ala 
            180                 185                 190         


Ser Thr Leu Asp Thr Phe Lys Ala Asn Glu Glu Gly Tyr Leu Gly Val 
        195                 200                 205             


Ile Ile Gly Asn Arg Ile Tyr Tyr Gln Asn Arg Ile Asp Lys Leu His 
    210                 215                 220                 


Thr Thr Arg Ser Val Phe Asp Val Arg Gly Leu Thr Ser Leu Pro Lys 
225                 230                 235                 240 


Val Asp Ile Leu Tyr Gly Tyr Gln Asp Asp Pro Glu Tyr Leu Tyr Asp 
                245                 250                 255     


Ala Ala Ile Gln His Gly Val Lys Gly Ile Val Tyr Ala Gly Met Gly 
            260                 265                 270         


Ala Gly Ser Val Ser Val Arg Gly Ile Ala Gly Met Arg Lys Ala Met 
        275                 280                 285             


Glu Lys Gly Val Val Val Ile Arg Ser Thr Arg Thr Gly Asn Gly Ile 
    290                 295                 300                 


Val Pro Pro Asp Glu Glu Leu Pro Gly Leu Val Ser Asp Ser Leu Asn 
305                 310                 315                 320 


Pro Ala His Ala Arg Ile Leu Leu Met Leu Ala Leu Thr Arg Thr Ser 
                325                 330                 335     


Asp Pro Lys Val Ile Gln Glu Tyr Phe His Thr Tyr 
            340                 345             


<210> 25
<211> 9
<212> PRT
<213> Homo sapiens

<400> 25
Ser Arg Arg Tyr Asp Ser Arg Thr Thr 
1               5                   


