			SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> ENGINEERED PURINE NUCLEOSIDE PHOSPHORYLASE VARIANT ENZYMES
 
<130> CX2-172WO1
 
<140> 
<141> 2019-07-02

<150> 62/695,507
<151> 2018-07-09

<150> 62/822,263
<151> 2019-03-22
  
<160> 1006

<210> 1
<211> 717
<212> DNA
<213> Artificial Sequence

<220>
<223> Codon optimized Purine Nucleoside Phosphorylase from E. Coli
 
<400> 1
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717


<210> 2
<211> 239
<212> PRT
<213> Escherichia coli 

<220>
<223> Purine Nucleoside Phosphorylase from E. Coli

<400> 2
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr
           20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 3
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Phosphopentomutase from Escherichia coli
 
<400> 3
atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180
ctggcgaaag cacacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccccgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgagt ggggatattt ctccgatcac 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccactc ttccggtacg gtcattctgg atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgctgact ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccagcgtac cggtaaccgt cacgacctgg ctgttgagcc gccagcaccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780
gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 4
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Phosphopentomutase from Escherichia coli
 
<400> 4
Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Thr Pro Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Trp Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys His Ser Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Asp Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg His Asp Leu Ala Val Glu Pro Pro Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405                                             

 
<210> 5
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 5
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 6
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 6
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 7
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 7
atggctaccc ctcacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacgacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 8
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 8
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 9
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 9
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccgcgtaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 10
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 10
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ala Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 11
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 11
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccacgtaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 12
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 12
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Thr Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 13
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 13
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgtca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgctg tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 14
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 14
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Ser Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Leu Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 15
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 15
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtacgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 16
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 16
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Thr Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 17
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 17
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcacggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 18
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 18
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Thr Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 19
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 19
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcttgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 20
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 20
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Leu Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 21
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 21
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 22
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 22
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 23
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 23
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccgtttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 24
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 24
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Val Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 25
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 25
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtag ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 26
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 26
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ala Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 27
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 27
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtccgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 28
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 28
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Pro Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 29
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 29
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgaagtgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 30
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 30
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Lys Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 31
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 31
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtagtggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 32
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 32
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ser Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 33
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 33
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacgcgccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 34
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 34
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ala Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 35
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 35
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactggt gagcagacca ccgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 36
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 36
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Gly Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 37
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 37
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttcagcgc ggacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cgccgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600
tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 38
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 38
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Ala Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 39
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 39
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactggt gagcagacca cctcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 40
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 40
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Gly Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 41
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 41
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
taccggccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 42
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 42
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Arg Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 43
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 43
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccggggttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 44
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 44
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Gly Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 45
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 45
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccactg tcgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 46
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 46
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Cys Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 47
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 47
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttcagcgc ggacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600
tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 48
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 48
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 49
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 49
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgttggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcgttct cgccgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600
tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 50
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 50
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Val 
                165                 170                 175     
Leu Ala Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 51
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 51
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgttggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcgttct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600
tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 52
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 52
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Val 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 53
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 53
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc atgaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 54
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 54
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Met Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 55
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 55
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cgccgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 56
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 56
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Ala Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 57
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 57
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg tgggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 58
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 58
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Val Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 59
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 59
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaacttttc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcactatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 60
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 60
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Thr Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 61
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 61
atgaataccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 62
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 62
Met Asn Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 63
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 63
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 64
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 64
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 65
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 65
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccctttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 66
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 66
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Leu Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 67
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 67
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 68
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 68
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 69
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 69
atggctaccc cacacattaa tgcagaaatg ggcaagttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcca cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 70
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 70
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Lys Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile His Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 71
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 71
atggctaccc cacacattaa tgcagaaatg ggctcgttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 72
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 72
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ser Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 73
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 73
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgttggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cgccgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600
tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 74
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 74
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Ala Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 75
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 75
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgttggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg ctggaaaaat acggcgttct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggccatc 600
tgtaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 76
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 76
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Leu Glu Lys Tyr Gly Val 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 77
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 77
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
ttggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 78
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 78
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Leu Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 79
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 79
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgt cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 80
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 80
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ser Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 81
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 81
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg agggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 82
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 82
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Glu Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 83
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 83
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccagggttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 84
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 84
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Arg Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 85
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 85
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gctggatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 86
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 86
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Trp Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 87
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 87
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcttgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 88
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 88
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Leu Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 89
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 89
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcgacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 90
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 90
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 91
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 91
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatgtaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctaggcga taaagag    717

 
<210> 92
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 92
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Met Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 93
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 93
atgtctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 94
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 94
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 95
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 95
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccgtggttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 96
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 96
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Val Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 97
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 97
atggctaccc cacacattaa tgcagaaatg ggctttttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 98
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 98
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Phe Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 99
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 99
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
cgggacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 100
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 100
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Arg Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 101
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 101
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcgttgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 102
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 102
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Val Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 103
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 103
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acgtgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 104
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 104
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 105
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 105
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacgacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacagcccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 106
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 106
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 107
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 107
atgccgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 108
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 108
Met Pro Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 109
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 109
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 110
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 110
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 111
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 111
atggctaccc cacacattaa tgcagaaatg ggcctgttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 112
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 112
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Leu Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 113
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 113
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacagcccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 114
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 114
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 115
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 115
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactggt gagcagacca ccgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 116
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 116
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Gly Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 117
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 117
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctacg ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcat gagcagacca gctcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 118
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 118
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Ala Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Ser Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 119
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 119
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacagcccgg acccagaaat gttcgacgtg atggaaaact acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactggt gagcagacca gcgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 120
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 120
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Pro Glu Met Phe Asp Val Met Glu Asn Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Gly Glu Gln Thr Ser Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 121
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 121
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctacg ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 122
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 122
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Ala Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     


<210> 123
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 123
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgttattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 124
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 124
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 125
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 125
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 126
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 126
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 127
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 127
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg cgggtatcca cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 128
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 128
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile His Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 129
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 129
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagtcctgcc gcacgtaaaa 300
ctgcgcgacg tcctgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 130
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 130
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Leu Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 131
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 131
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 132
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 132
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 133
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 133
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgttgaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat cggaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 134
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 134
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 135
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 135
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacgacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgttgaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 136
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 136
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 137
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 137
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggtattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 138
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 138
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 139
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 139
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 140
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 140
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 141
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 141
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg atggcgaaat gttcgacgtg atggaaaaat acgttattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 142
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 142
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 143
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 143
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300
gtgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 144
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 144
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 145
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 145
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 146
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 146
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 147
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 147
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggtattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 148
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 148
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 149
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 149
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 150
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 150
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 151
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 151
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 152
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 152
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 153
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 153
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgt cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 154
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 154
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ser Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 155
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 155
atgagcaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 156
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 156
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 157
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 157
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 158
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 158
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 159
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 159
atggctaccc cacacattaa tgcagaaatg ggcgacttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacacgccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggcgatc 600
tgcacggtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 160
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 160
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 161
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 161
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggcgatc 600
tgcacggtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 162
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 162
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 163
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 163
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 164
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 164
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 165
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 165
atggctaccc cacacattaa tgcagaaatg ggcgacttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt cccgtgttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 166
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 166
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Arg Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 167
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 167
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 168
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 168
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 169
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 169
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 170
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 170
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 171
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 171
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgtgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 172
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 172
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 173
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 173
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgttgaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtag cggaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 174
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 174
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ala Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 175
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 175
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagtcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 176
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 176
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 177
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 177
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagtcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 178
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 178
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 179
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 179
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 180
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 180
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 181
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 181
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tataggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgttgaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 182
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 182
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Ile Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 183
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 183
atgccgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 184
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 184
Met Pro Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 185
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 185
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcctgatcgg tatgggtgcc tgtaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 186
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 186
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Leu Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 187
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 187
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacgacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcactccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 188
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 188
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 189
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 189
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatttaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagtcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg atggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgt cgggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 190
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 190
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ser Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 191
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 191
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg caatcctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 192
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 192
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 193
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 193
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc cgaagaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca agggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaggaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 194
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 194
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Glu Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 195
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 195
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacgacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgttgaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 196
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 196
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 197
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 197
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtgg aaaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 198
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 198
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Glu Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 199
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 199
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgcgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttacg atggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga aaaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 200
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 200
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Asp Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 201
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 201
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccagggttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 202
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 202
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Arg Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 203
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 203
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gctggatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 204
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 204
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Trp Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 205
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 205
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcttgatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 206
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 206
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Leu Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 207
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 207
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcgacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 208
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 208
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 209
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 209
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatgtaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctaggcga taaagag    717

 
<210> 210
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 210
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Met Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 211
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 211
atgtctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 212
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 212
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 213
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 213
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccgtggttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 214
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 214
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Val Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 215
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 215
atggctaccc cacacattaa tgcagaaatg ggctttttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 216
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 216
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Phe Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 217
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 217
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
cgggacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 218
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 218
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Arg Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 219
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 219
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcgttgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 220
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 220
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Val Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 221
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 221
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acgtgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 222
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 222
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 223
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 223
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacgacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacagcccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 224
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 224
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asp Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 225
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 225
atgccgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 226
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 226
Met Pro Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 227
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 227
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 228
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 228
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 229
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 229
atggctaccc cacacattaa tgcagaaatg ggcctgttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 230
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 230
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Leu Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 231
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 231
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacagcccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 232
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 232
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 233
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 233
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactggt gagcagacca ccgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 234
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 234
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Gly Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 235
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 235
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctacg ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacaccccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcat gagcagacca gctcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 236
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 236
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Ala Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Thr Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Ser Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 237
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 237
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tacagcccgg acccagaaat gttcgacgtg atggaaaact acggcattct cggcgtggaa 540
atggaagcgg ctggtattta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactggt gagcagacca gcgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 238
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 238
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Pro Glu Met Phe Asp Val Met Glu Asn Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Gly Glu Gln Thr Ser Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 239
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 239
atggctaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggaatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtatgggtat cccgtcctgc tccatctacg ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 240
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 240
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Met Gly Ile Pro Ser Cys Ser Ile Tyr Ala Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Ser Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 241
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 241
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 242
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 242
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 243
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 243
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 244
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 244
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 245
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 245
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 246
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 246
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 247
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 247
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 248
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 248
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 249
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 249
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 250
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 250
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 251
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 251
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 252
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 252
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 253
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 253
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgtcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 254
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 254
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 255
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 255
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 256
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 256
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 257
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 257
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 258
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 258
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 259
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 259
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtattc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 260
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 260
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Ile Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 261
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 261
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgtcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 262
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 262
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 263
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 263
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggttatc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 264
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 264
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Tyr Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 265
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 265
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 266
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 266
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 267
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 267
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 268
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 268
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 269
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 269
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 270
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 270
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 271
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 271
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 272
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 272
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 273
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 273
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 274
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 274
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 275
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 275
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtacgc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 276
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 276
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Thr Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 277
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 277
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 278
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 278
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 279
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 279
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 280
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 280
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 281
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 281
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgtcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 282
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 282
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 283
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 283
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 284
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 284
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 285
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 285
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 286
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 286
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 287
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 287
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 288
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 288
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 289
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 289
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 290
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 290
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 291
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 291
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 292
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 292
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 293
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 293
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 294
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 294
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 295
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 295
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 296
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 296
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 297
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 297
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcctggga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 298
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 298
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Trp Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 299
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 299
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 300
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 300
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 301
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 301
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 302
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 302
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 303
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 303
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 304
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 304
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 305
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 305
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 306
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 306
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 307
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 307
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 308
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 308
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 309
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 309
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 310
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 310
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 311
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 311
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtcctc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 312
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 312
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Pro Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 313
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 313
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 314
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 314
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 315
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 315
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 316
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 316
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 317
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 317
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 318
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 318
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 319
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 319
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 320
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 320
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 321
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 321
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtcgtc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 322
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 322
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Arg Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 323
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 323
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggttgtc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 324
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 324
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Cys Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 325
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 325
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gtttgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 326
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 326
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 327
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 327
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgcaggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 328
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 328
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 329
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 329
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 330
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 330
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 331
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 331
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccctgga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 332
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 332
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Leu Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 333
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 333
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 334
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 334
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 335
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 335
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 336
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 336
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 337
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 337
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 338
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 338
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 339
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 339
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 340
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 340
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 341
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 341
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct cgtttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 342
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 342
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Arg Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 343
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 343
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 344
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 344
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 345
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 345
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 346
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 346
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 347
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 347
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct atgttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 348
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 348
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Met Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 349
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 349
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 350
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 350
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 351
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 351
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatccct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 352
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 352
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Pro Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 353
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 353
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gcatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 354
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 354
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys His Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 355
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 355
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggttctc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 356
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 356
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Ser Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 357
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 357
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcctatga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 358
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 358
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Tyr Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 359
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 359
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtttgc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 360
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 360
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Leu Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 361
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 361
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 362
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 362
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 363
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 363
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 364
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 364
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 365
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 365
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 366
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 366
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 367
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 367
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatggct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 368
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 368
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Met Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 369
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 369
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 370
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 370
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 371
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 371
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgattaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 372
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 372
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Ile Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 373
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 373
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcctttga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 374
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 374
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Phe Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 375
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 375
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 376
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 376
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 377
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 377
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 378
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 378
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 379
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 379
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 380
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 380
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 381
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 381
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 382
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 382
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 383
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 383
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtggctaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 384
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 384
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Ala Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 385
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 385
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 386
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 386
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 387
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 387
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 388
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 388
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 389
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 389
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactggt gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 390
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 390
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Gly Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 391
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 391
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttccacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 392
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 392
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe His Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 393
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 393
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgttgaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 394
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 394
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Leu Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 395
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 395
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 396
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 396
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 397
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 397
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctgag gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 398
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 398
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Glu Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 399
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 399
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 400
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 400
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 401
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 401
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 402
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 402
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 403
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 403
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct ggtgaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 404
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 404
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Gly Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 405
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 405
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgagtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 406
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 406
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Ser Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 407
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 407
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc aatggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 408
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 408
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Asn Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 409
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 409
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaagagt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 410
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 410
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Glu Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 411
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 411
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 412
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 412
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 413
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 413
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 414
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 414
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 415
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 415
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 416
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 416
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 417
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 417
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct tatgaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 418
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 418
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Tyr Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 419
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 419
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc ctgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 420
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 420
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Leu Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 421
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 421
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcact gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 422
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 422
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Thr Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 423
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 423
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggttaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 424
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 424
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Val Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 425
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 425
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 426
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 426
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 427
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 427
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 428
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 428
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 429
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 429
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgtcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 430
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 430
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Val Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 431
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 431
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgcttctgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 432
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 432
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ser Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 433
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 433
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaatggt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 434
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 434
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Trp Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 435
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 435
atgtcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 436
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 436
Met Ser Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 437
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 437
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 438
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 438
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 439
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 439
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat tcgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcggttgc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 440
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 440
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Val Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 441
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 441
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 442
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 442
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 443
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 443
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 444
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 444
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 445
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 445
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 446
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 446
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 447
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 447
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 448
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 448
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 449
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 449
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct tgggaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 450
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 450
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Trp Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 451
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 451
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 452
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 452
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 453
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 453
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgtgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 454
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 454
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Cys Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 455
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 455
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 456
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 456
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 457
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 457
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtggtc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 458
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 458
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Gly Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 459
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 459
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc tttggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 460
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 460
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Phe Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 461
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 461
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcctgtga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 462
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 462
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Cys Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 463
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 463
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttg 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 464
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 464
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Leu 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 465
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 465
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc cagggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 466
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 466
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Gln Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 467
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 467
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 468
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 468
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 469
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 469
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatgcgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 470
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 470
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Met Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 471
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 471
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggagaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 472
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 472
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Glu Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 473
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 473
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct tttgaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 474
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 474
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Phe Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 475
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 475
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 476
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 476
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 477
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 477
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgcttat gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 478
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 478
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Tyr Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 479
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 479
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 480
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 480
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 481
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 481
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tagggctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgacattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 482
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 482
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Arg Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Asp Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 483
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 483
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct cctgaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 484
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 484
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Pro Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 485
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 485
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcgc tatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgacattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 486
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 486
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Ala Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Asp Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 487
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 487
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaaactc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 488
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 488
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Thr His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 489
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 489
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaacatt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 490
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 490
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu His Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 491
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 491
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gaccttgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 492
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 492
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Leu Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 493
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 493
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gactttgaca tggtgcgtaa cgcagtagat 420
gcagctgaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 494
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 494
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Glu Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 495
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 495
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagtggacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 496
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 496
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Trp Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 497
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 497
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct actgaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcaa taaagag    717

 
<210> 498
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 498
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Thr Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asn Lys Glu     
225                 230                 235                     

 
<210> 499
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 499
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaatcgt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 500
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 500
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Ser Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 501
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 501
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagcttgtg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 502
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 502
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Cys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 503
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 503
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 504
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 504
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 505
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 505
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgaataacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 506
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 506
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Asn Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 507
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 507
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgaccgg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccatat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 508
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 508
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Arg Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Ile Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 509
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 509
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 510
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 510
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 511
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 511
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat tccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaatttt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 512
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 512
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Phe Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 513
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 513
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaagggt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 514
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 514
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Gly Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 515
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 515
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggataa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 516
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 516
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Asp Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 517
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 517
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 518
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 518
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 519
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 519
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagttt 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 520
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 520
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Phe Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 521
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 521
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agactattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 522
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 522
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Thr Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 523
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 523
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctggggct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 524
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 524
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Gly Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 525
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 525
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccac tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacgcggaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 526
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 526
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Ala Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 527
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 527
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgcccggga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 528
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 528
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Arg Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 529
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 529
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc catggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 530
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 530
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg His Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 531
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 531
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gactgtgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 532
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 532
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Cys Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 533
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 533
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gtggaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 534
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 534
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Val Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 535
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 535
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagatgat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 536
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 536
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Asp Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 537
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 537
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcgcgatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 538
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 538
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Ala Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 539
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 539
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgttg ggcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 540
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 540
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Trp Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 541
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 541
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 542
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 542
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 543
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 543
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 544
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 544
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 545
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 545
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaacagt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 546
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 546
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Gln Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 547
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 547
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gctgtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 548
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 548
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Cys Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 549
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 549
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tatggctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 550
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 550
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Met Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 551
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 551
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctgtgg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 552
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 552
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Val Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 553
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 553
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctcggg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 554
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 554
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Arg Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 555
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 555
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 556
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 556
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 557
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 557
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tattgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgacattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 558
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 558
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Ile Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Asp Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 559
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 559
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgagtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 560
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 560
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Ser Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 561
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 561
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcaacggat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 562
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 562
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Thr Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 563
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 563
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aaccatgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 564
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 564
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn His Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 565
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 565
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaat atctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 566
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 566
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Tyr 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 567
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 567
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaagttt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 568
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 568
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Val Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 569
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 569
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gactctgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 570
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 570
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Ser Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 571
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 571
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 572
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 572
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 573
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 573
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggcgaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 574
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 574
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Ala Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 575
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 575
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 576
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 576
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 577
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 577
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 578
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 578
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 579
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 579
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca cctcggaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 580
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 580
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Ser Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 581
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 581
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagaca gggactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 582
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 582
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp Arg Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 583
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 583
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactagg gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 584
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 584
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Arg Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 585
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 585
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaaa ctctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 586
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 586
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Thr 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 587
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 587
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat tccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaat tgctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 588
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 588
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Leu 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 589
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 589
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctatg gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 590
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 590
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Met Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 591
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 591
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaat ttctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 592
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 592
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Phe 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 593
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 593
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 594
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 594
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 595
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 595
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcaagtgat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 596
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 596
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Ser Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 597
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 597
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtga ggcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 598
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 598
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Glu Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 599
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 599
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
attcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 600
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 600
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Ile Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 601
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 601
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tggtgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 602
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 602
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Gly Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 603
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 603
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtactctga aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 604
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 604
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Leu Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 605
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 605
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca atggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaggtt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 606
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 606
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Asn Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Gly Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 607
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 607
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttttccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgactatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 608
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 608
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 609
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 609
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagggc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 610
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 610
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Gly His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 611
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 611
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgc gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 612
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 612
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Ala Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 613
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 613
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaaatgc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 614
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 614
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Met His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 615
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 615
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaac ggctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 616
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 616
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Arg 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 617
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 617
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagcggat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 618
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 618
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Ala Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 619
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 619
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctggtg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 620
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 620
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Gly Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 621
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 621
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaaagtc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 622
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 622
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Ser His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 623
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 623
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 624
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 624
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 625
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 625
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acgacattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 626
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 626
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Asp Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 627
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 627
atggcgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgaa 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg caattctgcc gcacgtaaaa 300
gtgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctggctatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcaaacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 628
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 628
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Glu 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Ile Leu 
                85                  90                  95      
Pro His Val Lys Val Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 629
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 629
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtggggaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 630
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 630
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Gly Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 631
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 631
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcgtgcgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 632
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 632
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Val Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 633
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 633
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacagtgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 634
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 634
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Ser Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 635
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 635
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gtatgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 636
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 636
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro Tyr Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 637
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 637
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga aggcgattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 638
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 638
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Ala Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 639
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 639
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaatgtt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 640
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 640
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Cys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 641
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 641
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaatgt acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 642
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 642
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Met Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 643
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 643
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattac gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 644
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 644
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Thr Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 645
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 645
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtca ggcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 646
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 646
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Gln Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 647
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 647
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcacgcgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 648
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 648
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Thr Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 649
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 649
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtaggg 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 650
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 650
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Gly Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 651
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 651
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagcttatg cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 652
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 652
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Tyr Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 653
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 653
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacaatgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 654
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 654
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Asn Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 655
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 655
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcaggggat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 656
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 656
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Gly Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 657
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 657
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaac atctgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 658
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 658
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys His 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 659
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 659
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat ttttgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 660
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 660
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Phe Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 661
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 661
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtctggtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 662
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 662
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Leu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 663
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 663
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgg tggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 664
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 664
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Gly Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 665
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 665
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtgg agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcacccac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 666
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 666
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Glu Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 667
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 667
atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc ggatgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgttctccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattct cggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 668
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 668
Met Ala Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro Asp Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu Phe Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Leu Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gln Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 669
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 669
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcaggct 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 670
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 670
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Ala Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 671
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 671
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gactattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 672
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 672
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Thr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 673
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 673
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcgcgg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 674
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 674
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Ala Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 675
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 675
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gccgaatttg gctcgaaggc cctggccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 676
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 676
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ser Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 677
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 677
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac aatgctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 678
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 678
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Asn Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 679
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 679
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctccggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 680
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 680
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Pro Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 681
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 681
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggcttcta ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 682
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 682
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Ser Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 683
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 683
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 684
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 684
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 685
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 685
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cagcgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 686
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 686
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Ala Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 687
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 687
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat caagactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 688
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 688
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Lys 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 689
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 689
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctat gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 690
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 690
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Met Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 691
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 691
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg gtatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 692
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 692
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Gly 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 693
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 693
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagggg 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 694
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 694
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Gly Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 695
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 695
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg ctatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 696
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 696
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Ala 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 697
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 697
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgccatcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 698
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 698
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
His His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 699
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 699
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagcggacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 700
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 700
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Arg Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 701
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 701
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cacagggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 702
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 702
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Gln Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 703
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 703
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcac tatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 704
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 704
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Thr Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 705
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 705
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
agtttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 706
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 706
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Ser Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 707
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 707
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgat 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgcaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggcaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gccgaatttg gcgcgaaggc cctggccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggccga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 708
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 708
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ala Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 709
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 709
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctaccc atgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 710
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 710
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr His Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 711
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 711
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tacatgccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 712
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 712
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Met Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 713
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 713
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag catttggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 714
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 714
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Phe Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 715
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 715
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctaccg ttgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 716
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 716
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Val Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 717
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 717
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atgcgtaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 718
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 718
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Arg Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 719
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 719
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagtcg 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 720
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 720
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Ser Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 721
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 721
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcctgg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 722
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 722
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Leu Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 723
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 723
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat cactactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 724
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 724
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Thr 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 725
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 725
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcgggcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 726
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 726
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Gly His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 727
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 727
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaaa cgctgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 728
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 728
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Thr 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 729
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 729
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga tcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 730
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 730
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Asp Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 731
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 731
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat ttcggctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 732
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 732
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Ser Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 733
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 733
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccacactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga tccggag    717

 
<210> 734
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 734
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile His 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Pro Glu     
225                 230                 235                     

 
<210> 735
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 735
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac actgctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 736
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 736
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Thr Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 737
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 737
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaggtttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 738
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 738
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Gly 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 739
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 739
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtgcgact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 740
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 740
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Ala Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 741
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 741
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa ggctattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 742
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 742
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Ala Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 743
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 743
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctcgtg cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 744
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 744
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Arg Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 745
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 745
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttccatgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 746
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 746
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe His Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 747
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 747
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gcttattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 748
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 748
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Leu Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 749
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 749
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcttgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 750
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 750
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Leu Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 751
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 751
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag caagtggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 752
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 752
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Ser Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 753
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 753
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac caggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 754
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 754
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Gln Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 755
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 755
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggca cggttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 756
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 756
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Thr Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 757
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 757
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggttacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 758
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 758
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Val Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 759
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 759
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtagct 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 760
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 760
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Ala Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 761
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 761
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccatactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 762
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 762
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile His 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 763
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 763
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaacagttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 764
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 764
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Gln 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 765
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 765
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct ctgactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 766
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 766
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Leu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 767
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 767
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagcgt 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 768
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 768
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Arg Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 769
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 769
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac agtgctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 770
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 770
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Ser Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 771
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 771
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcctgcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 772
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 772
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Leu His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 773
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 773
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaaa ggctgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 774
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 774
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Arg 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 775
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 775
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tattgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 776
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 776
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Ile Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 777
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 777
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgatgggcga taaagag    717

 
<210> 778
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 778
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Met Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 779
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 779
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctggggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 780
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 780
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Gly Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 781
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 781
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaaca agatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 782
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 782
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Lys 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 783
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 783
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttccgggtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 784
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 784
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Arg Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 785
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 785
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgctctcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 786
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 786
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Ser His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 787
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 787
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgactgt atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 788
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 788
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Cys Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 789
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 789
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttctata tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 790
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 790
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Tyr Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 791
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 791
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gggtattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 792
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 792
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Gly Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 793
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 793
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaaca atatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 794
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 794
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asn 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 795
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 795
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaacgtt acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 796
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 796
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Arg Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 797
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 797
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgcta tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 798
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 798
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Ala Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 799
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 799
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctcatgctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 800
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 800
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr His Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 801
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 801
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct tggactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 802
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 802
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Trp Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 803
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 803
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcggcggc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 804
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 804
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Ala Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 805
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 805
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcagtgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 806
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 806
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Ser Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 807
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 807
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctca gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 808
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 808
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Gln Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 809
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 809
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatct gcgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 810
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 810
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Leu Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 811
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 811
atgacgaccc cacacattta tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 812
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 812
Met Thr Thr Pro His Ile Tyr Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 813
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 813
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gaatgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 814
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 814
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro Asn Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 815
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 815
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctggc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 816
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 816
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Ala His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 817
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 817
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ctttactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 818
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 818
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Phe 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 819
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 819
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac tatgctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 820
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 820
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Tyr Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 821
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 821
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctgtt gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 822
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 822
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Val Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 823
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 823
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactttt gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 824
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 824
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Phe Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 825
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 825
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga tccggag    717

 
<210> 826
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 826
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Pro Glu     
225                 230                 235                     

 
<210> 827
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 827
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgctggcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 828
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 828
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Trp His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 829
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 829
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctgc tcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 830
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 830
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Ala Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 831
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 831
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgaagaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 832
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 832
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Lys Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 833
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 833
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gtgtgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 834
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 834
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro Cys Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 835
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 835
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc ggctgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 836
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 836
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro Ala Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 837
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 837
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca cttcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 838
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 838
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ser Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 839
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 839
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagggtacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 840
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 840
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Gly Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 841
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 841
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttagggatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 842
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 842
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Arg Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 843
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 843
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc ggatgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 844
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 844
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro Asp Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 845
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 845
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacct gcgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 846
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 846
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Leu Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 847
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 847
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgctt gatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 848
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 848
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Leu Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 849
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 849
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgtt ttatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 850
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 850
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Phe Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 851
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 851
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgctttcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 852
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 852
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Phe His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 853
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 853
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaac atctgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 854
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 854
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys His 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 855
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 855
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaagtgttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 856
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 856
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Val 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 857
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 857
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtgtgtaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 858
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 858
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Val Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 859
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 859
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggttattaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 860
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 860
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Tyr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 861
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 861
atgacgaccc cacacattaa tgcagaaatg ggcgctttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 862
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 862
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Ala Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 863
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 863
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cacatggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 864
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 864
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
His Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 865
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 865
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat cagtactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 866
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 866
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Ser 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 867
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 867
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcat gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 868
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 868
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala His Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 869
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 869
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctaccg ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 870
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 870
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Ala Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 871
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 871
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct actactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 872
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 872
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Thr Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 873
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 873
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccttgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 874
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 874
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Leu Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 875
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 875
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga aggcgattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 876
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 876
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Ala Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 877
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 877
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcta tatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 878
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 878
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Tyr Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 879
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 879
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgag ttatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 880
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 880
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Ser Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 881
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 881
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttctgga tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 882
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 882
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Trp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 883
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 883
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ctatgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 884
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 884
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Tyr Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 885
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 885
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtatat 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 886
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 886
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Tyr Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 887
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 887
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcgttg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 888
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 888
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Val Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 889
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 889
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgtc tcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatgctcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 890
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 890
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Ser His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Ala Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 891
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 891
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtcaggtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc tgacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 892
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 892
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Gln Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro Asp Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 893
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 893
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcacggac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc tcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcga gatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 894
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 894
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Glu Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 895
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 895
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg ttcgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 896
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 896
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Ser Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 897
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 897
atgacgaccc cacacattaa tgcagaacct ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 898
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 898
Met Thr Thr Pro His Ile Asn Ala Glu Pro Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 899
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 899
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
cactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 900
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 900
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
His Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 901
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 901
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcacgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 902
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 902
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Thr Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 903
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 903
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acacggaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 904
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 904
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Thr Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 905
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 905
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgacctggc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 906
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 906
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Leu Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 907
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 907
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgga tgacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa gatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 908
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 908
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Asp Asp Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 909
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 909
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acagggaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 910
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 910
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Arg Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 911
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 911
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatggcg 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 912
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 912
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Ala Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 913
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 913
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tcagggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 914
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 914
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Gln Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 915
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 915
atgacgaccc cacacattaa tgcagaacct ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcacggac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag caaagggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcga gatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 916
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 916
Met Thr Thr Pro His Ile Asn Ala Glu Pro Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Lys Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Glu Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 917
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 917
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atggcgcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 918
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 918
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Ala His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 919
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 919
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccact 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 920
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 920
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Thr Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 921
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 921
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cacagggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg atatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 922
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 922
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Gln Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 923
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 923
atgacgaccc cacacattaa tgcagaacct ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcggca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcga gatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 924
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 924
Met Thr Thr Pro His Ile Asn Ala Glu Pro Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Gly Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Glu Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 925
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 925
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat caagactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 926
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 926
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Lys 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 927
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 927
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcacggac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcggca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 928
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 928
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Gly Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 929
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 929
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagtctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 930
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 930
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ser Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 931
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 931
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctacg ttaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 932
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 932
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Val Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 933
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 933
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggca cggttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 934
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 934
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Thr Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 935
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 935
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat cctgactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 936
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 936
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Leu 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 937
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 937
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acagtgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 938
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 938
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Ser Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 939
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 939
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tgctgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 940
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 940
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Leu Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 941
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 941
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgagtatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 942
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 942
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Ser Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 943
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 943
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg accttgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 944
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 944
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Leu Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 945
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 945
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtcaggtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcggca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcga gatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 946
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 946
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Gln Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Gly Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Glu Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 947
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 947
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggca gtgttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 948
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 948
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ser Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 949
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 949
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcattggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 950
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 950
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Ile Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 951
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 951
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca gtaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 952
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 952
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Ser Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 953
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 953
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acgcggaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 954
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 954
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Ala Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 955
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 955
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tatgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 956
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 956
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Met Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 957
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 957
atgacgaccc cacacattaa tgcagaacct ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag caaagggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa gatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 958
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 958
Met Thr Thr Pro His Ile Asn Ala Glu Pro Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Lys Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 959
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 959
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg actgtgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 960
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 960
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Cys Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 961
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 961
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggttctg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 962
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 962
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly Ser Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 963
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 963
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttggacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 964
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 964
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Leu Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 965
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 965
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat cgttactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 966
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 966
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Val 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 967
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 967
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ctttactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 968
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 968
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Phe 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 969
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 969
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg accatgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 970
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 970
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp His Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 971
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 971
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggttacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 972
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 972
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Val Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 973
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 973
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg accctgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 974
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 974
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Pro Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 975
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 975
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gagatgacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 976
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 976
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Met Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 977
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 977
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaggtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgga tcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa gatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 978
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 978
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Asp His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 979
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 979
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgggt 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 980
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 980
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Gly Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 981
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 981
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaact gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 982
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 982
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Leu Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 983
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 983
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactttccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 984
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 984
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Phe Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 985
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 985
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tgcgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 986
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 986
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Ala Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 987
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 987
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattat gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 988
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 988
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Met Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 989
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 989
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactatg gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 990
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 990
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr Met Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 991
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 991
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatacggct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 992
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 992
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Thr Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 993
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 993
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatcttgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 994
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 994
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Leu Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 995
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 995
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg gtatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 996
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 996
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Gly 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 997
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 997
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tgtgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 998
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 998
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Val Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 999
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 999
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gacggacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 1000
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 1000
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Thr Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 
<210> 1001
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 1001
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgttcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 1002
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 1002
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ser Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     


<210> 1003
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phosphopentomutase from Escherichia coli
 
<400> 1003
atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180
ctggcgaaag catatgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga taccctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt ctccgatcac 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccacgc gtccggtacg gtcattctgg aacaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattacctgc 540
catgaagaaa ctttcggtct ggataaactc tacgaactgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccagcgtac cggtaaccgt cgtgacctgg ctgttgagcc gccagcaccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgt cggtaaaatt 780
gcggacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtac tgaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ttctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 1004
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phosphopentomutase from Escherichia coli
 
<400> 1004
Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
Tyr Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Thr Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Ser Asp His Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys His Ala Ser Gly Thr Val Ile Leu Glu Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Thr Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Pro Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 
                245                 250                 255     
Val Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Thr Gly Thr Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405                                             

 
<210> 1005
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phosphopentomutase from Escherichia coli
 
<400> 1005
atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgctgcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctgttgagcc gggggcaccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttctgc gggtaaaatt 780
gccgacatct acgccaactg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
aacttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 1006
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phosphopentomutase from Escherichia coli
 
<400> 1006
Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Val Glu Pro Gly Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 
                245                 250                 255     
Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Cys Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Asn Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405                                             
