			SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> ENGINEERED PHENYLALANINE AMMONIA LYASE POLYPEPTIDES
 
<130> CX2-179WO1
 
<140> 
<141> 2019-06-14

<150> 62/696,978
<151> 2018-07-12

<150> 62/814,362
<151> 2019-03-06
 
<160> 1222
 
<210> 1
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild Type Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1
atgaaaaccc tgagccaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgcacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gcaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggtt tctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccaat agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatca tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctgct gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga cctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 2
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild Type Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 2
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Ala Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Ala Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp Phe Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Asn Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Leu Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Thr Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 3
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 3
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 4
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 4
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 5
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 5
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggcc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 6
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 6
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 7
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 7
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 8
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 8
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 9
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 9
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggcc 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 10
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 10
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 11
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 11
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggcc 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 12
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 12
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 13
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 13
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 14
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 14
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 15
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 15
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgatctg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 16
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 16
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Asp Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 17
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 17
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 18
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 18
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 19
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 19
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgatctg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctgaccccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 20
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 20
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Asp Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 21
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 21
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 22
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 22
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 23
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 23
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggcc 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 24
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 24
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 25
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 25
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggccccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactggcg caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 26
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 26
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Ala Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ala Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 27
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 27
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggcc 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 28
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 28
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 29
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 29
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggccccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactggcg caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 30
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 30
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Ala Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ala Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 31
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 31
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctgaccccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggcc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 32
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 32
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 33
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 33
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggccccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagtgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 34
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 34
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Ala Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 35
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 35
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtcca ctgacccagc tgccgaaaga aggtctggcc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 36
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 36
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Gln Leu Pro Lys Glu Gly Leu Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 37
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 37
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctgaccccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggcc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 38
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 38
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Thr Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 39
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 39
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggccccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 40
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 40
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Ala Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 41
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 41
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggccccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 42
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 42
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Ala Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 43
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 43
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaata ttgtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 44
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 44
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ile Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 45
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 45
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg ctgtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 46
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 46
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 47
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 47
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaaagg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 48
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 48
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Arg Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 49
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 49
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaatcg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 50
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 50
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Ser Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 51
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 51
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggac tctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 52
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 52
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp Thr Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 53
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 53
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt ccgcagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 54
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 54
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Pro Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 55
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 55
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtggggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 56
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 56
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Gly Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 57
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 57
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtg ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 58
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 58
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Val Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 59
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 59
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtccggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gttggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 60
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 60
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Pro Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 61
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 61
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatttggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 62
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 62
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Leu 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 63
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 63
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtatggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgga 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 64
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 64
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Met Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Gly His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 65
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 65
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatt cggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 66
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 66
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ser Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 67
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 67
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatgtt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 68
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 68
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Val Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 69
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 69
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 70
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 70
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 71
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 71
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcaggagt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 72
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 72
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Glu Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 73
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 73
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aacgcgaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 74
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 74
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Ala Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 75
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 75
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgaggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 76
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 76
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Glu 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 77
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 77
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tgggaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 78
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 78
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Gly Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 79
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 79
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cgagaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 80
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 80
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Glu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 81
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 81
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgggttatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atcgcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 82
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 82
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Gly Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Arg Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 83
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 83
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaccc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctggatggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 84
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 84
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Pro Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Asp Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 85
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 85
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc ctgtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 86
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 86
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Pro Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 87
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 87
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgatgt gccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 88
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 88
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Val Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 89
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 89
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatacg gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 90
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 90
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Thr Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 91
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 91
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
aagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 92
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 92
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Lys Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 93
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 93
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgccg 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 94
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 94
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Pro Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 95
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 95
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgcgtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 96
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 96
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Arg Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 97
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 97
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaatcgtc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 98
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 98
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Arg Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 99
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 99
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatc tggtttggca cctgaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgagctatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctggca 660
atgatgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacaggag aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 100
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 100
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Leu Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ala Met Met Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr His Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Glu Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 101
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 101
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacagac cagcgaactg 300
cagaccaatg cgatttgggg acttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 102
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 102
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Thr Ser Glu Leu Gln Thr Asn Ala Ile Trp Gly Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 103
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 103
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 104
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 104
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 105
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 105
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 106
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 106
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 107
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 107
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
caggagaatg cggtttgggc acttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 108
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 108
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Val Trp Ala Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 109
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 109
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcagct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgattcgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggttaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaagc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 110
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 110
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Ser Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Arg Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Ser Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 111
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 111
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagaccaatg cgatttgggg acttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaagc 1260
ctgcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 112
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 112
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp Gly Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Ser Leu Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 113
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 113
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatggggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 114
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 114
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Gly 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 115
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 115
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggttaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt accgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaggc 1260
ctgcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 116
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 116
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 117
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 117
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
caggagaatg cgatttggct ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 118
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 118
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Leu Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 119
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 119
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 120
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 120
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 121
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 121
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcagct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggca ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggttaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaagc 1260
ctgcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 122
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 122
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Ser Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Ser Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 123
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 123
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg atgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgattcggct ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 124
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 124
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Asp Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Arg Leu Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 125
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 125
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg atgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacagac cagcgaactg 300
cagaccaatg cgattcgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggttaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 126
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 126
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Asp Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Thr Ser Glu Leu Gln Thr Asn Ala Ile Arg Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 127
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 127
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagaccaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaggc 1260
ctgcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 128
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 128
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 129
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 129
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg atgaaccgat ttatggtgcg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagaccaatg cgatttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaagc 1260
ctgcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 130
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 130
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Asp Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Ser Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 131
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 131
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg atgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgattcgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagtggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt accgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 132
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 132
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Asp Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Arg Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 133
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 133
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggttgtggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 134
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 134
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 135
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 135
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtg ggggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 136
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 136
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Gly Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 137
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 137
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagaccaatg cgattcgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaagc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 138
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 138
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Arg Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Ser Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 139
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 139
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacagac cagcgaactg 300
caggagaatg cgattcgggg acttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggttaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt accgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaagc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 140
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 140
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Thr Ser Glu Leu Gln Glu Asn Ala Ile Arg Gly Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Ser Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 141
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 141
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
caggagaatg cggtttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggttaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaagc 1260
ctgcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 142
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 142
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Val Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Ser Leu Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 143
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 143
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagaccaatg cggtttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 144
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 144
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 145
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 145
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggct ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
ctgcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 146
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 146
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Leu Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 147
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 147
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg atgaaccgat ttatggtgtg 240
accagcagct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggctaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaggc 1260
ctgcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 148
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 148
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Asp Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Ser Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Ala Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 149
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 149
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagaccaatg cgatttggat ccttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 150
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 150
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp Ile Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 151
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 151
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggac ccttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgattccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 152
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 152
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Thr Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ile Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 153
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 153
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacagac cagcgaactg 300
caggagaatg cggtttggca ccttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt accgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaagc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 154
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 154
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Thr Ser Glu Leu Gln Glu Asn Ala Val Trp His Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Ser Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 155
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 155
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacagac cagcgaactg 300
cagaccaatg cgatttggag ccttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 156
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 156
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Thr Ser Glu Leu Gln Thr Asn Ala Ile Trp Ser Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 157
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 157
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgtggg tctgaaaagc 1260
ctgcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 158
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 158
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Ser Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 159
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 159
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagaccaatg cgatttggga acttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 160
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 160
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp Glu Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 161
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 161
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacagac cagcgaactg 300
caggagaatg cgatttgggg acttaaaacc gccgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg cttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 162
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 162
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Thr Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 163
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 163
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtg ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgatctt 300
cagaccaata taatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtggaggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 164
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 164
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Val Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Asp Leu Gln Thr Asn Ile Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Gly Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 165
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 165
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggat ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
ctgcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 166
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 166
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ile Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 167
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 167
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttgggg tcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 168
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 168
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 169
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 169
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggctaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 170
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 170
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Ala Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 171
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 171
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgaattatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 172
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 172
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Asn Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 173
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 173
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg gggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 174
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 174
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Gly Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 175
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 175
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgcg 240
accagcggtg ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgatctt 300
cagaccaata taatttggac tcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtggaggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgata caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 176
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 176
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Ala 
65                  70                  75                  80  
Thr Ser Gly Val Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Asp Leu Gln Thr Asn Ile Ile Trp Thr Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Gly Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 177
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 177
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggct gcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 178
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 178
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Leu Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 179
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 179
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagacctctg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 180
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 180
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Ser Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 181
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 181
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgggtga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 182
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 182
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 183
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 183
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtg ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgatctt 300
cagaccaatg cagtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgata caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 184
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 184
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Val Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Asp Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 185
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 185
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca gcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 186
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 186
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Gln Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 187
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 187
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtg ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cagtttggac tcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgata caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 188
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 188
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Val Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Thr Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 189
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 189
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttgggg gcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt aaccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 190
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 190
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Asn Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 191
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 191
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtg ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 192
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 192
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Val Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 193
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 193
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt tgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 194
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 194
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Leu Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 195
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 195
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatcatgg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 196
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 196
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn His 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 197
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 197
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgttgg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 198
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 198
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Val 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 199
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 199
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaagct 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 200
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 200
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Ala Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 201
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 201
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatgctgg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 202
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 202
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Ala 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 203
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 203
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtgagg ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 204
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 204
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Glu Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 205
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 205
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtctggg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 206
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 206
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Leu Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 207
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 207
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg tttcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 208
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 208
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Val Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 209
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 209
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactt 300
cagaccaatg caatttggac tcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggttccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgata caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 210
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 210
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp Thr Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 211
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 211
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtcttggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 212
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 212
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 213
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 213
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtt cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 214
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 214
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Ser Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 215
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 215
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtt 240
accagcggtc ggggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 216
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 216
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Arg Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 217
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 217
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccgac 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtcttgg tgaactgatc caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc agtgtctgca t                                           1701

 
<210> 218
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 218
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Asp Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Leu Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Gln Cys Leu His                                     
                565                                             

 
<210> 219
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 219
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggcc gcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 220
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 220
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Pro Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 221
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 221
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggttatgca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 222
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 222
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Met His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 223
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 223
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg caatgccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 224
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 224
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Met Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 225
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 225
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgatctt 300
cagaccaatg caatttggac tcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 226
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 226
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Asp Leu Gln Thr Asn Ala Ile Trp Thr Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 227
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 227
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtggaggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 228
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 228
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Gly Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 229
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 229
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtactg ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 230
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 230
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Thr Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 231
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 231
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtggaggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 232
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 232
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Gly Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 233
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 233
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccac atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcgtggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 234
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 234
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Arg Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 235
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 235
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaattgtgg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 236
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 236
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Cys 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 237
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 237
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaataatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 238
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 238
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Asn Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 239
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 239
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaatct 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 240
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 240
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Ser Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 241
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 241
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cgatttggca ccttaaaacc gctgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtggaggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 242
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 242
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Ile Trp His Leu Lys Thr Ala Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Gly Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 243
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 243
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatctggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 244
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 244
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Leu 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 245
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 245
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tattaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 246
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 246
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Ile Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 247
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 247
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggca ccttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgtcg 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 248
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 248
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp His Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 249
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 249
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggtt ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc aagcgaactg 300
cagaccaatg cggtttggac tcttaaaacc ggtgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt atcgtgatgg tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgg catcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggt 1260
ctgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 250
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 250
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Thr Asn Ala Val Trp Thr Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Tyr 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ala Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Leu Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 251
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 251
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 252
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 252
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 253
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 253
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggtttaagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataaa gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 254
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 254
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 255
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 255
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtttaagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctatcgaggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tcttaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 256
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 256
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Arg Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 257
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 257
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggaaaacg cgatttgggg tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggtttaagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tcttaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 258
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 258
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 259
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 259
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgagttatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctcggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 260
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 260
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 261
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 261
atgaaaacca gtagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 262
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 262
Met Lys Thr Ser Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 263
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 263
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaactat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 264
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 264
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Thr Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 265
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 265
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 266
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 266
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 267
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 267
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggtttaggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataaa gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 268
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 268
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 269
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 269
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggaaaacg cgatttgggg tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgggtga aggtttaggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataaa gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 270
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 270
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 271
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 271
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgagttatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggttgtggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 272
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 272
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 273
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 273
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataaa gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 274
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 274
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 275
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 275
atgaaagagc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 276
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 276
Met Lys Glu Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 277
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 277
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacttat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 278
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 278
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 279
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 279
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 280
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 280
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 281
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 281
atgaaaaccc tgagtcaggc acagagcagt accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggcaagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 282
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 282
Met Lys Thr Leu Ser Gln Ala Gln Ser Ser Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 283
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 283
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatattt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 284
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 284
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 285
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 285
atgaaaaccc tgagtgatgc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 286
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 286
Met Lys Thr Leu Ser Asp Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 287
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 287
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgagttatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggttgtggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 288
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 288
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 289
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 289
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaaggat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 290
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 290
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Arg Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 291
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 291
atgaaaccac tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 292
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 292
Met Lys Pro Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 293
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 293
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 294
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 294
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 295
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 295
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtgggca t                                           1701

 
<210> 296
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 296
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 297
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 297
atgaaagagc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattaca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 298
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 298
Met Lys Glu Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Thr Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 299
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 299
atgaaaaccc tggatcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 300
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 300
Met Lys Thr Leu Asp Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 301
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 301
atgaaaaccc tgagtcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 302
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 302
Met Lys Thr Leu Ser Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 303
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 303
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgagttatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctcggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 304
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 304
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 305
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 305
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatgtgagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaatcgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 306
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 306
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Val Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Ser Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 307
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 307
atgaaaaagc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 308
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 308
Met Lys Lys Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 309
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 309
atgaaacgtc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 310
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 310
Met Lys Arg Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 311
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 311
atgaaaaccc ctagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 312
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 312
Met Lys Thr Pro Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 313
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 313
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagaacaacg cgatttgggg tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataaa gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 314
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 314
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Asn Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 315
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 315
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg ttaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 316
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 316
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Val Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 317
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 317
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 318
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 318
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 319
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 319
atgaaaaccc tgccgcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 320
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 320
Met Lys Thr Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 321
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 321
atgaaaaccc tgagtcaggg acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 322
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 322
Met Lys Thr Leu Ser Gln Gly Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 323
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 323
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggttgtggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 324
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 324
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 325
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 325
atgaaacatc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 326
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 326
Met Lys His Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 327
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 327
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgga t                                           1701

 
<210> 328
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 328
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu Asp                                     
                565                                             

 
<210> 329
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 329
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaagagat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 330
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 330
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Glu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 331
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 331
atgaaaaccc tgagtcaggc acagagcaaa accagcagcg cgcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 332
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 332
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Ala Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 333
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 333
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggcaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatacgt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 334
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 334
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Thr Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 335
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 335
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg cacgggtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 336
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 336
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Arg Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 337
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 337
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgcctctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 338
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 338
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Pro Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 339
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 339
atgaaaaccc tgagtcaggc acagagcgcg accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 340
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 340
Met Lys Thr Leu Ser Gln Ala Gln Ser Ala Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 341
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 341
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
tctcatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 342
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 342
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 343
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 343
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgttt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 344
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 344
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Val Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 345
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 345
atgaaaaccc tgagtcagac tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 346
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 346
Met Lys Thr Leu Ser Gln Thr Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 347
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 347
atgaaaaccc tgagttcggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 348
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 348
Met Lys Thr Leu Ser Ser Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 349
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 349
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacataagt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 350
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 350
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Lys Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 351
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 351
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattcggct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 352
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 352
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Arg Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 353
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 353
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaagcgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 354
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 354
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Ala Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 355
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 355
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtacgtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 356
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 356
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Thr Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 357
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 357
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaactgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
cttattctgc cgtgtctgca t                                           1701

 
<210> 358
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 358
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Leu Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 359
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 359
atgaaaaccc tgttgcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 360
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 360
Met Lys Thr Leu Leu Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 361
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 361
atgaaaaccc tgagtcaggc acagagccct accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 362
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 362
Met Lys Thr Leu Ser Gln Ala Gln Ser Pro Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 363
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 363
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacataggt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 364
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 364
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Arg Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 365
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 365
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcat ggattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 366
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 366
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Trp 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 367
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 367
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtctgccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 368
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 368
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Leu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 369
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 369
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaatctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 370
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 370
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Asn Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 371
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 371
atgaaaaatc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 372
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 372
Met Lys Asn Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 373
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 373
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatgcgagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggtctgggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 374
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 374
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ala Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Leu Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 375
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 375
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggttgtggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 376
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 376
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 377
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 377
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tggcttatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggttgtggc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca t                                           1701

 
<210> 378
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 378
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ala Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Gly Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 379
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 379
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 380
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 380
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 381
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 381
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacctat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 382
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 382
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 383
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 383
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggtc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 384
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 384
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 385
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 385
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 386
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 386
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 387
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 387
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 388
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 388
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 389
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 389
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaacgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 390
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 390
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Thr Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 391
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 391
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacttat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 392
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 392
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 393
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 393
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 394
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 394
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 395
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 395
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg cgaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtttaca ttaa                                        1704

 
<210> 396
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 396
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 397
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 397
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacttat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 398
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 398
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 399
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 399
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 400
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 400
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 401
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 401
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 402
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 402
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 403
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 403
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
tcgcatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca ttaa                                        1704

 
<210> 404
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 404
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 405
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 405
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc cgggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 406
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 406
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 407
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 407
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaacgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg acttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 408
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 408
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Thr Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 409
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 409
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtttaca ttaa                                        1704

 
<210> 410
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 410
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 411
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 411
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct tcggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 412
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 412
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 413
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 413
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaacgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca tgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 414
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 414
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Thr Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 415
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 415
atgaaagagt cgccgcaggg tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg cgaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaacaat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 416
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 416
Met Lys Glu Ser Pro Gln Gly Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Thr Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 417
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 417
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacttat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 418
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 418
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 419
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 419
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg tgaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaagcaat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 420
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 420
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Val Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Ala Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 421
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 421
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca ttaa                                        1704

 
<210> 422
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 422
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 423
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 423
atgaaaaccc tgagtcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg tgaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggg tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 424
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 424
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Val Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Gly Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 425
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 425
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca ttaa                                        1704

 
<210> 426
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 426
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 427
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 427
atgaaaaccc tgagtcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg cgaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaagcaat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 428
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 428
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Ala Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 429
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 429
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
tcgcatgatt ggcgtgatca cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 430
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 430
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Ser His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 431
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 431
atgaaaaccc tgagtcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 432
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 432
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 433
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 433
atgaaaaccc tgagtcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg cgaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatca cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 434
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 434
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp His Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 435
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 435
atgaaagagc tgtcgcaggg tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag tcttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 436
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 436
Met Lys Glu Leu Ser Gln Gly Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 437
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 437
atgaaaaccc tgagtcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg cgaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttggag ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 438
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 438
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ser Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 439
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 439
atgaaaaagc tgtcgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaactgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca ttaa                                        1704

 
<210> 440
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 440
Met Lys Lys Leu Ser Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 441
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 441
atgaaaaccc tgagtcaggc acagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg cgaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggcc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcctgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca ttaa                                        1704

 
<210> 442
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 442
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 443
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 443
atgaaaaccc tgagtcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaacaat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtctgca ttaa                                        1704

 
<210> 444
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 444
Met Lys Thr Leu Ser Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Thr Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Leu His                                     
                565                                             

 
<210> 445
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 445
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca ttaa                                        1704

 
<210> 446
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 446
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 447
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 447
atgaaagagt tgccgtcggc ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 448
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 448
Met Lys Glu Leu Pro Ser Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 449
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 449
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacga 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatatat ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 450
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 450
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 451
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 451
atgaaacatt cgccgcaggg ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacga 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 452
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 452
Met Lys His Ser Pro Gln Gly Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 453
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 453
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacga 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaagccat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 454
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 454
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Ala Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 455
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 455
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacgtat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtcagtgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 456
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 456
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Arg Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Gln Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 457
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 457
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatatat ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 458
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 458
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 459
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 459
atgaaacatt tgccgcagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacgtat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 460
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 460
Met Lys His Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Arg Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 461
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 461
atgaaagagt cgccgcaggt ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacga 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 462
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 462
Met Lys Glu Ser Pro Gln Val Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 463
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 463
atgaaaaagt cgccgtcggt ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacga 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatatat ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 464
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 464
Met Lys Lys Ser Pro Ser Val Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 465
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 465
atgaaacatt tgccgcagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacga 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 466
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 466
Met Lys His Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 467
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 467
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatatat ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 468
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 468
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 469
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 469
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacgtat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 470
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 470
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Arg Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 471
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 471
atgaaaacct cgccggatga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacgtat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 472
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 472
Met Lys Thr Ser Pro Asp Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Arg Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 473
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 473
atgaaacctt tgccgcaggg ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatatat ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 474
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 474
Met Lys Pro Leu Pro Gln Gly Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 475
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 475
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 476
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 476
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 477
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 477
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacttat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 478
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 478
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 479
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 479
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 480
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 480
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 481
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 481
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 482
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 482
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 483
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 483
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgactaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 484
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 484
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Thr Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 485
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 485
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accacgggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 486
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 486
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 487
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 487
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat cctgtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 488
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 488
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Pro Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 489
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 489
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgacgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccaaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 490
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 490
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Thr Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Lys Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 491
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 491
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaacat 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 492
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 492
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu His Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 493
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 493
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat gtggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 494
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 494
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Val Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 495
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 495
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gttcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 496
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 496
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 497
<211> 1704
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 497
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggtacgagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca ttaa                                        1704

 
<210> 498
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 498
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Thr Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 499
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 499
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggtatgagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 500
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 500
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Met Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 501
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 501
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg tttcggataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 502
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 502
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Ser Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 503
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 503
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccggggga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 504
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 504
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 505
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 505
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga agcgtgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 506
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 506
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Ala Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 507
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 507
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatc atcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 508
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 508
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp His 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 509
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 509
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatg tgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 510
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 510
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Val 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 511
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 511
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
caggagaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ttcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 512
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 512
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Glu Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Phe 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 513
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 513
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaactcat ttatggtgtg 240
accaccggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atgacaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatatct ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 514
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 514
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Thr Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 515
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 515
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 516
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 516
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 517
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 517
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgact ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 518
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 518
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 519
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 519
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accaccggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 520
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 520
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 521
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 521
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaactcat ttatggtgtg 240
accaccggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgggaga agcctgcagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 522
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 522
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Leu Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Ala Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 523
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 523
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accaccggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 524
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 524
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 525
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 525
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgggaga aggctgcagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 526
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 526
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 527
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 527
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgggaga aggcacgagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 528
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 528
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Gly Thr Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 529
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 529
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacccat ttatggtgtg 240
accaccggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgact ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 530
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 530
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 531
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 531
atgaaagaat cgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 532
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 532
Met Lys Glu Ser Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 533
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 533
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ccggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 534
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 534
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Pro Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 535
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 535
atgaaacctt cgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgggaga agcctgcagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgact ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 536
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 536
Met Lys Pro Ser Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Ala Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 537
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 537
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggccttggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 538
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 538
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Leu 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 539
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 539
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatttt 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 540
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 540
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Phe Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 541
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 541
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgggaga agcctgcagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 542
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 542
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Ala Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 543
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 543
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 544
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 544
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 545
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 545
atgaaagaat tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 546
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 546
Met Lys Glu Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 547
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 547
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggcaccagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 548
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 548
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Thr Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 549
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 549
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgtcagc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 550
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 550
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Gln Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 551
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 551
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaaaga aggcacgagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatatct ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 552
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 552
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Thr Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 553
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 553
atgaaacctt cgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgact ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 554
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 554
Met Lys Pro Ser Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 555
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 555
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgggaga aggctgcagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 556
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 556
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Gly Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 557
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 557
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 558
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 558
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 559
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 559
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 560
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 560
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 561
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 561
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cttgatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 562
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 562
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Leu Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 563
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 563
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggcaccagc 660
atgggaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttagcgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 564
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 564
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Thr Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Ser Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 565
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 565
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tgttgatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 566
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 566
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Val Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 567
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 567
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtca tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 568
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 568
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly His Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 569
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 569
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattcagc gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 570
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 570
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Gln Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 571
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 571
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gctaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 572
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 572
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 573
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 573
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 574
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 574
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 575
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 575
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggttcgta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 576
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 576
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Ser Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 577
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 577
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg attcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 578
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 578
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Asp Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 579
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 579
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggctctggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 580
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 580
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ser 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 581
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 581
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagt attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 582
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 582
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Tyr Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 583
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 583
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cttcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 584
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 584
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 585
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 585
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgc ggtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 586
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 586
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Arg Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 587
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 587
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcacgggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 588
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 588
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Thr 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 589
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 589
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tactcataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 590
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 590
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Thr His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 591
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 591
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccca ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 592
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 592
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Gln Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 593
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 593
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag catgatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 594
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 594
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Met Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 595
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 595
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcaactc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 596
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 596
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Thr Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 597
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 597
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattggat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 598
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 598
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Trp Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 599
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 599
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tattgatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 600
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 600
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Ile Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 601
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 601
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt actcagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 602
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 602
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Thr Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 603
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 603
atgaaacctt tgccgcaggc tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaccgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgc tgtcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctggcgccg ctgaccctgc tgccgaagga aggttgcagc 660
atgggtaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 604
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 604
Met Lys Pro Leu Pro Gln Ala Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Pro Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Leu Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ala Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Gly Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 605
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 605
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 606
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 606
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 607
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 607
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaaccttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg taattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 608
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 608
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Thr Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 609
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 609
atgaaacctc tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 610
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 610
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 611
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 611
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta acggcaccaa ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccctgcgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 612
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 612
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Leu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 613
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 613
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 614
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 614
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 615
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 615
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttagtgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga actatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 616
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 616
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Ser Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 617
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 617
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 618
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 618
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 619
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 619
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgata gtcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 620
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 620
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 621
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 621
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cctgatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga actatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 622
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 622
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Leu Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 623
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 623
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccatcag agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 624
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 624
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 625
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 625
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga agggtgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttagtgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 626
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 626
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Ser Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 627
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 627
atgaaacctc tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cctgatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 628
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 628
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Leu Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 629
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 629
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttagtgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag catcatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 630
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 630
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Ser Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 631
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 631
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcgc gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 632
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 632
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 633
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 633
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag catcatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 634
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 634
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 635
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 635
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatattt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 636
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 636
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Ile Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 637
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 637
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 638
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 638
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 639
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 639
atgaaaccta ttccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt gtcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 640
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 640
Met Lys Pro Ile Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Cys 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 641
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 641
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccaa ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg taattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 642
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 642
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 643
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 643
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta acggcaccaa ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg taattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 644
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 644
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 645
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 645
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccaa ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccctgcgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 646
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 646
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Leu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 647
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 647
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcctgggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgact ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 648
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 648
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Leu 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 649
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 649
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagac 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gttcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 650
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 650
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 651
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 651
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacatat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 652
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 652
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 653
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 653
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagac 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtgaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtca tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 654
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 654
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly His Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 655
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 655
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcgaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg taattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 656
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 656
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Glu Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 657
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 657
atgaaacctt tgccgcagtc gcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 658
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 658
Met Lys Pro Leu Pro Gln Ser Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 659
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 659
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta acggcaccaa ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcgaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 660
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 660
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Glu Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 661
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 661
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatc atcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 662
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 662
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp His 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 663
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 663
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatc ttcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 664
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 664
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Leu 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 665
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 665
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg cagcggtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgtagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 666
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 666
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Ala Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 667
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 667
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtaaggg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 668
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 668
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Lys Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 669
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 669
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg gccaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgattggtgc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 670
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 670
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Ala Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Gly Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 671
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 671
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtactg ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 672
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 672
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Thr Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 673
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 673
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc caatgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gttgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 674
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 674
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Asn Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 675
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 675
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtatag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 676
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 676
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Tyr 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 677
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 677
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cgaggaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 678
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 678
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 679
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 679
atgaaacctt tgccgcggga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 680
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 680
Met Lys Pro Leu Pro Arg Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 681
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 681
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 682
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 682
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 683
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 683
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accactggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 684
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 684
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 685
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 685
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttgggacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 686
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 686
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Gly Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 687
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 687
atgaaacctg ggccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 688
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 688
Met Lys Pro Gly Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 689
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 689
atgaaacctt tgccgcagga tcagtgtaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 690
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 690
Met Lys Pro Leu Pro Gln Asp Gln Cys Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 691
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 691
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatactgg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 692
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 692
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Thr 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 693
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 693
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caggggtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 694
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 694
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Gly Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 695
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 695
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag cttgtatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 696
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 696
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Leu Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 697
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 697
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cgcggaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 698
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 698
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ala Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 699
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 699
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgattatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 700
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 700
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Ile Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 701
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 701
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccacg 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 702
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 702
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Thr Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 703
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 703
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacgggatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 704
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 704
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys Arg Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 705
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 705
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtctag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 706
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 706
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Ser 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 707
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 707
atgaaacctt tgccgcagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggct ttggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtagtg ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 708
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 708
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Phe Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Ser Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 709
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 709
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 710
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 710
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 711
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 711
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtacag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cgaagaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgctggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 712
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 712
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Tyr 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 713
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 713
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 714
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 714
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 715
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 715
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtaaggg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 716
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 716
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Lys Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 717
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 717
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagttcag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtca cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 718
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 718
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly His Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 719
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 719
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgctggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtca cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 720
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 720
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly His Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 721
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 721
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 722
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 722
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 723
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 723
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cgaagaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 724
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 724
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 725
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 725
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatc accgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccctgcgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 726
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 726
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp His 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Leu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 727
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 727
atgaaacctt tgccacagtc gcagagcaaa accagcagcc agcagttcag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cgaagaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 728
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 728
Met Lys Pro Leu Pro Gln Ser Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 729
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 729
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accacgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 730
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 730
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 731
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 731
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accacgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 732
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 732
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 733
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 733
atgaaaccta ttccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accacgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 734
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 734
Met Lys Pro Ile Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 735
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 735
atgaaacctc tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 736
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 736
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 737
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 737
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 738
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 738
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 739
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 739
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagttcag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataat gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 740
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 740
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 741
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 741
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc caacgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 742
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 742
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Asn Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 743
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 743
atgaaacctt tgccacagtc gcagagcaaa accagcagcc agcagttcag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc caacgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgctggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 744
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 744
Met Lys Pro Leu Pro Gln Ser Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Asn Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 745
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 745
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cgaagaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgctggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccctgcgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 746
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 746
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Leu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 747
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 747
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcctgggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 748
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 748
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Leu 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 749
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 749
atgaaacctc tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtaaggg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atatgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 750
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 750
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Lys Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Met Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 751
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 751
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtacag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cgaagaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgctggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 752
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 752
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Tyr 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 753
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 753
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cgaagaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtaaggg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 754
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 754
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Lys Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 755
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 755
atgaaacctc tgccacagag ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accacgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 756
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 756
Met Lys Pro Leu Pro Gln Ser Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 757
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 757
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cgaagaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgctggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccatcaa tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 758
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 758
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Gln Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 759
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 759
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcctgggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 760
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 760
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Leu 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 761
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 761
atgaaacctc tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 762
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 762
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 763
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 763
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cgcagaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 764
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 764
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ala Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 765
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 765
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtacag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 766
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 766
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Tyr 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 767
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 767
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgtc acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgctggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataat gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtca cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 768
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 768
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg His Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly His Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 769
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 769
atgaaacctt tgccacagga ccagagcaaa accagcagcc agcagttcag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta acggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 770
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 770
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 771
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 771
atgaaacctt tgccacagtc gcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgt cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 772
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 772
Met Lys Pro Leu Pro Gln Ser Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 773
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 773
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata aagatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctggaaccg ctgcaactgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aagcgggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gcgggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 774
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 774
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Lys Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Gln Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Ala Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 775
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 775
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 776
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 776
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 777
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 777
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata aagatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgactctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 778
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 778
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Lys Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 779
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 779
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata aagatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgcaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgactctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gcgggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 780
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 780
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Lys Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Ala Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 781
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 781
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataatg aagatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctggaaccg ctgcaactgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca tcgggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 782
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 782
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Glu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Gln Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ser Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 783
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 783
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtggg cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgcaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctggg cctggaaccg ctgcaactgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aagcgggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gcgggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 784
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 784
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Gly Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Ala Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Gly Leu 
        195                 200                 205             
Glu Pro Leu Gln Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Ala Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 785
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 785
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataatg aagatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgcaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctggaaccg ctgcaactgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 786
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 786
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Glu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Ala Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Gln Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 787
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 787
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accacgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 788
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 788
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 789
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 789
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataatg aagatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgcaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 790
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 790
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Glu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Ala Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 791
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 791
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgcaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaatcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaacgggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gcgggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 792
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 792
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Ala Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 793
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 793
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctggaaccg ctgactctgc taccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaatcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aagcgggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca tcgggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 794
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 794
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Ala Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ser Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 795
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 795
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataatg aagatattct gcagggtatt 180
caggccagct gtgattatat caacaatgca gttgcaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaacgggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 796
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 796
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Glu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Ala Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 797
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 797
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaac cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat cgccaatgca gttgcaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcacgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctggaaccg ctgcaactgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aagcgggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca tcgggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 798
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 798
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Ala Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Gln Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Ala Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ser Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 799
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 799
atgaaacctc tgccacagga ccagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accacgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 800
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 800
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Thr Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 801
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 801
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcgcg 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 802
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 802
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Ala 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 803
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 803
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttcgtctg accaataata ccgatattct gcagggcatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta agaaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgataccaag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
aaaaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 804
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 804
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Arg Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Lys Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Lys 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Lys Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 805
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 805
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggaggat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 806
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 806
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Glu Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 807
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 807
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ctcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 808
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 808
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Ala Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 809
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 809
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaat ttattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 810
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 810
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ile Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 811
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 811
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttatgca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 812
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 812
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Met His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 813
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 813
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggtggat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 814
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 814
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Val Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 815
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 815
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatgt gccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 816
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 816
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Val Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 817
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 817
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cggagggaca t                                           1701

 
<210> 818
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 818
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Glu Gly His                                     
                565                                             

 
<210> 819
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 819
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccct ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaaatgat ttatggtgtg 240
accagcggcc ctggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgt gtcgtgatga actggatggt 900
aaacatgatt ggcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccga gctcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 820
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 820
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Leu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu Met Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Cys Arg Asp Glu Leu Asp Gly Lys His Asp Trp 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ser Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 821
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 821
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctggg tctgagcccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgct 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 822
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 822
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Gly Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 823
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 823
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta acggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 824
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 824
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 825
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 825
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat cgcaaatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagttgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgc gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 826
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 826
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 827
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 827
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatg aagatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 828
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 828
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Glu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 829
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 829
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 830
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 830
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 831
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 831
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caacaatgca gttgcaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 832
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 832
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Ala Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 833
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 833
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgagcccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgata gtcgtgaggg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagttgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgc gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 834
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 834
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Glu Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 835
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 835
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta acggcaccaa ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat cgcaaatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cgcggaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 836
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 836
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ala Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 837
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 837
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 838
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 838
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 839
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 839
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caacaatgca gttgcaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagtgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagttgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgct 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 840
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 840
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Ala Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 841
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 841
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc ctgaacaggc cagtgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagttgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 842
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 842
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Pro Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 843
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 843
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgagcccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 844
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 844
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 845
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 845
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta acggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatc atcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 846
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 846
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp His 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 847
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 847
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatg aagatattct gcagggtatt 180
caggccagct gtgattatat cgcgaatgca gttgcaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cgaggaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctggagccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 848
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 848
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Glu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Ala Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 849
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 849
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgcttc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 850
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 850
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Leu Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 851
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 851
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtagat 120
gttgcccgta acggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat cgcaaatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgata gtcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgc gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgct 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 852
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 852
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Asp Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 853
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 853
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
accagcggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagttgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgc gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 854
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 854
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 855
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 855
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgcctc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 856
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 856
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 857
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 857
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgtagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgga gtgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 858
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 858
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Glu Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 859
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 859
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caacaatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 860
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 860
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 861
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 861
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc ctcggaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 862
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 862
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 863
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 863
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgct 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 864
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 864
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 865
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 865
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta acggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc ctcggaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctggg tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 866
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 866
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Gly Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 867
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 867
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta acggcaccaa agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cgaggaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagttgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 868
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 868
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Lys Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Glu Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 869
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 869
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctggg tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgacgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagttgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 870
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 870
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Gly Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 871
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 871
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 872
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 872
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 873
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 873
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caactgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagtgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 874
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 874
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 875
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 875
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 876
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 876
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 877
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 877
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatattgg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 878
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 878
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Ile 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 879
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 879
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgt cgtcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 880
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 880
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Ser Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 881
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 881
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaaggt 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 882
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 882
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Gly Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 883
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 883
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgct 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 884
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 884
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 885
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 885
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga ggttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 886
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 886
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 887
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 887
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtattaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 888
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 888
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ile Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 889
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 889
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta acggcacccc agttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat cgcgaatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 890
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 890
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Ala Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 891
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 891
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata ccgatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatacc 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca tgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 892
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 892
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Thr Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 893
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 893
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 894
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 894
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 895
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 895
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ctgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 896
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 896
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 897
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 897
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 898
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 898
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 899
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 899
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggtc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ctgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 900
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 900
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 901
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 901
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgctgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ctgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaatcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 902
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 902
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Leu Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 903
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 903
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaatcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 904
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 904
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 905
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 905
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatattgg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 906
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 906
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Ile 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 907
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 907
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaatcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 908
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 908
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 909
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 909
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ctgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatattgg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 910
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 910
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Ile 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 911
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 911
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 912
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 912
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 913
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 913
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac tcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatattgg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 914
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 914
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Ile 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 915
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 915
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaatcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcgt gaacgtaaag ttaatattgg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 916
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 916
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Ile 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 917
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 917
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggtc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatattgg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 918
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 918
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Ile 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 919
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 919
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gcatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gcgaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 920
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 920
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro His Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 921
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 921
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 922
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 922
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 923
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 923
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 924
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 924
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 925
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 925
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgctcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ctgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 926
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 926
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 927
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 927
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atcatacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 928
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 928
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn His Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 929
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 929
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttgct 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 930
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 930
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Ala Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 931
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 931
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgccgccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 932
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 932
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Pro Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 933
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 933
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgctcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 934
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 934
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 935
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 935
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgtgat gggtgttcat gcactggata tacaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 936
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 936
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Val Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 937
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 937
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgactccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 938
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 938
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Thr Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 939
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 939
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctgcaggat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 940
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 940
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Gln Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 941
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 941
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagccag 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 942
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 942
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Gln 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 943
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 943
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtgcgt 120
gttgcccgta atggcacccc ggttagcctg accaataata aggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagtg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgctgagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgcgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 944
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 944
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Lys Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Leu Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 945
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 945
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtaggg 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 946
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 946
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Gly Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 947
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 947
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg cgggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 948
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 948
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 949
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 949
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata aggatattct gcagcgtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 950
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 950
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Lys Asp Ile Leu Gln Arg Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 951
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 951
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac gctaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 952
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 952
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ala Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 953
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 953
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttttgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 954
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 954
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Phe Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 955
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 955
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacct ctattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 956
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 956
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 957
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 957
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgcctgatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 958
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 958
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Pro Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 959
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 959
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacagcc tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 960
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 960
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Pro Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 961
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 961
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc catttcgctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 962
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 962
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ser Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 963
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 963
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagatgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 964
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 964
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Met Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 965
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 965
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatgag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 966
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 966
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Glu Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 967
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 967
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgaagggaca t                                           1701

 
<210> 968
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 968
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Lys Gly His                                     
                565                                             

 
<210> 969
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 969
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatg ctcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 970
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 970
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ala 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 971
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 971
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatgcg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 972
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 972
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Ala Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 973
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 973
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggc gctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 974
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 974
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Ala Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 975
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 975
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcgcg 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 976
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 976
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Ala 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 977
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 977
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta ttttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 978
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 978
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Phe 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 979
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 979
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgggcc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa tctgagtccg ctgaccctgc tgccgaagga aggctgcagc 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga actcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataga gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtca cgttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacgtgctgc cgtgtggaca t                                           1701

 
<210> 980
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 980
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Gly Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Ser Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ser Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Arg Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg His Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Val Leu Pro Cys Gly His                                     
                565                                             

 
<210> 981
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 981
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataata aggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgccaccg ctgaccctga atccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacca gcattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaac 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcaaccc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 982
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 982
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Lys Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Pro Pro Leu Thr Leu Asn Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Thr Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 983
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 983
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgccaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcaaccc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 984
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 984
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Pro Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Thr Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 985
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 985
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg ctattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaac 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgcc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 986
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 986
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 987
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 987
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 988
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 988
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 989
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 989
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgtgat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 990
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 990
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Val Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 991
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 991
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacaa 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgccaccg ctgaccctga atccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacca gcattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaac 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgcc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttgcc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 992
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 992
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Gln Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Pro Pro Leu Thr Leu Asn Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Ala Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 993
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 993
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 994
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 994
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 995
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 995
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaatcgg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttgcc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 996
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 996
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Ala Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 997
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 997
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtaggc 120
gttgcccgta atcacacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggccgat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgcctccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcgat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 998
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 998
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Gly Val Ala Arg Asn His Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Pro Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 999
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 999
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacaa 120
gttgcccgta atggcacccg tgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctga atccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaatcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaac 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcaaccc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1000
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1000
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Gln Val Ala Arg Asn Gly Thr Arg Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Asn Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Thr Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1001
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1001
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtaggc 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgcctccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgtgat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1002
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1002
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Gly Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Pro Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Val Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1003
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1003
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgccaccg ctgaccctga atccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacca gcattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaac 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgcc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1004
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1004
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Pro Pro Leu Thr Leu Asn Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1005
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1005
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtaacg 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctga atccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacca gcattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaagtcgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaac 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcaaccc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1006
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1006
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Thr Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Asn Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Val Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Thr Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1007
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1007
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaaaag gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctgactccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcgat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttgcc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1008
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1008
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Lys Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Thr Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Ala Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1009
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1009
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaccgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atcacacccc ggttagcctg accaataatc tggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaaaag gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggccgat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgtgat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1010
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1010
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Thr Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn His Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Leu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Lys Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Val Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1011
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1011
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgtgat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg tgggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1012
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1012
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Val Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1013
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1013
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggccgat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcgat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1014
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1014
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1015
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1015
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1016
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1016
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1017
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1017
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtagtg ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1018
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1018
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Ser Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1019
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1019
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg ggaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1020
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1020
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1021
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1021
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1022
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1022
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ser Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1023
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1023
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ggggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1024
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1024
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Gly Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1025
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1025
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc ctccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt tctgatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1026
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1026
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro Pro Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Ser Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1027
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1027
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatcag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1028
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1028
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Gln Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1029
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1029
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataata cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggaaccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gctcgtaaag ttaatatggg tctgaaaggc 1260
actcagattg taggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggt 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1030
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1030
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Thr Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Ala Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Val Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1031
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1031
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcatctc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1032
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1032
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Ser Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1033
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1033
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaatg tgaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1034
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1034
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Val Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1035
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1035
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
acgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1036
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1036
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1037
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1037
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatcag gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
tcgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1038
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1038
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Gln Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Ser Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1039
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1039
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcagggc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1040
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1040
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Gly Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1041
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1041
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaatc tgaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1042
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1042
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Leu Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1043
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1043
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc tggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
acgcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1044
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1044
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Leu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1045
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1045
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1046
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1046
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1047
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1047
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1048
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1048
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1049
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1049
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
acgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1050
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1050
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1051
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1051
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcagcgc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1052
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1052
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Ala Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1053
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1053
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctga atccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
acgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1054
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1054
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Asn Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1055
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1055
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaatc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1056
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1056
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Asn Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1057
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1057
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caagatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
tcgcagattg gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1058
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1058
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Ser Gln Ile Gly Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1059
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1059
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttcggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt catgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacct cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtt gtattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1060
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1060
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Ser Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Cys Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1061
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1061
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc tgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttcggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt catgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacct cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1062
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1062
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Ser Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1063
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1063
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcatggc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1064
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1064
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Trp Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1065
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1065
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcattgc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1066
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1066
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Leu Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1067
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1067
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1068
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1068
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1069
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1069
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc tggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgc 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caagatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
tcgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1070
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1070
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Leu Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Ser Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1071
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1071
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgc 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caagatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
tcgcagattt gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1072
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1072
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Ser Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1073
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1073
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcagttc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1074
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1074
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Val Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1075
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1075
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatcag gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgc 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
acgcagattg gcggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1076
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1076
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Gln Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Gly Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1077
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1077
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaatgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc tgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttcggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt catgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtt gtattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1078
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1078
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Ser Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Cys Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1079
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1079
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaacct gtgcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1080
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1080
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Cys 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1081
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1081
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
tcgcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1082
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1082
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Ser Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1083
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1083
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattcagc gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1084
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1084
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Gln Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1085
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1085
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc tgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgattcgtgc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt catgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1086
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1086
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Arg Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1087
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1087
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc aggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1088
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1088
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Gln 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1089
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1089
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaatggag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1090
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1090
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Met Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1091
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1091
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1092
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1092
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1093
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1093
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1094
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1094
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1095
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1095
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga tgttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1096
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1096
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Asp Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1097
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1097
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccga tttcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1098
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1098
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ile Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1099
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1099
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg gtatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1100
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1100
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Gly Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1101
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1101
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgggttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1102
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1102
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Gly Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1103
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1103
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ggggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1104
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1104
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Gly Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1105
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1105
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccga ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1106
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1106
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Glu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1107
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1107
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccac ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1108
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1108
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Thr Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1109
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1109
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtt tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1110
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1110
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Leu Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1111
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1111
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctgc tgtcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1112
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1112
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Leu Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1113
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1113
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
cagcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggcc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1114
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1114
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Gln Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1115
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1115
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatact gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1116
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1116
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Thr Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1117
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1117
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatgtt gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1118
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1118
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Val Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1119
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1119
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaattat gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1120
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1120
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Tyr Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1121
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1121
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcaccgc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1122
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1122
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Ala Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1123
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1123
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccg tgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1124
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1124
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Arg Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1125
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1125
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatttg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1126
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1126
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Leu Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1127
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1127
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt tgggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1128
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1128
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Leu Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1129
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1129
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg ttattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1130
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1130
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1131
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1131
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagagtaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1132
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1132
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Ser Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1133
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1133
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgcca cggttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1134
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1134
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Thr Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1135
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1135
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatccagga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1136
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1136
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Gln Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1137
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1137
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatctgtga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1138
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1138
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Cys Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1139
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1139
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1140
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1140
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1141
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1141
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1142
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1142
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1143
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1143
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacct cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc acctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gcacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcgc gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1144
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1144
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Ala Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1145
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1145
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gcacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1146
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1146
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Ala Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Gly Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1147
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1147
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1148
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1148
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1149
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1149
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacct cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattcagc gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1150
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1150
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Gln Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1151
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1151
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1152
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1152
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1153
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1153
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcactgc aggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1154
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1154
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Leu Gln 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1155
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1155
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc acctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtgg tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1156
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1156
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Gly Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1157
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1157
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacct cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattcagc gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1158
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1158
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Gln Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1159
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1159
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtgaa ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1160
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1160
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Glu Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1161
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1161
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaatg ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatca tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1162
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1162
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Val Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp His Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1163
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1163
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaactggag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga actatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcgc gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1164
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1164
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Leu Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1165
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1165
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1166
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1166
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1167
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1167
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatg acctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1168
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1168
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp Asp Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1169
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1169
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatg acctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1170
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1170
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp Asp Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1171
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1171
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaactggag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattcagc gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1172
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1172
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Leu Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Gln Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1173
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1173
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacct cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gcacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1174
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1174
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Ala Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1175
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1175
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaactggag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtgccg ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtgg tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1176
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1176
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Leu Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Ala Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Gly Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1177
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1177
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcgc gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1178
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1178
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Gly Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1179
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1179
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattcagc gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1180
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1180
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Gln Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1181
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1181
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgacct cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1182
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1182
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ser Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1183
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1183
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaactggag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc acctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1184
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1184
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Leu Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1185
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1185
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gcacgtaaag ttaatatggg tctgaaaggc 1260
actcagattt gtggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1186
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1186
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Ala Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Cys Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1187
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1187
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa cagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcagcgt gtgcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1188
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1188
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Ala Cys 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1189
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1189
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaactggag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1190
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1190
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Leu Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1191
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1191
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc acctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga actatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1192
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1192
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1193
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1193
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc acctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaccg ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1194
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1194
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Thr Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1195
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1195
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtacgt 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattatat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagctt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg caattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatatg gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg ctggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaactc tggcacgtcg tagcgttgat atttttcaga attatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1196
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1196
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Arg Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Tyr Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Phe Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Met Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Asn Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1197
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1197
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatccaaga tgttgtatgc 120
gttgcccgta atggcacccg cgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg ccatctgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattc tgggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga tgttgttggc 1560
cagaaaccgg catcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1198
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1198
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Gln Asp Val Val Cys Val Ala Arg Asn Gly Thr Arg Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Leu Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Asp Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1199
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1199
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatacg gaacgtaagg ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga tgttgttggc 1560
cagaaaccgg catcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1200
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1200
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Thr Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Asp Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1201
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1201
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcaccga ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gggttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccga tttcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1202
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1202
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Glu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ile Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1203
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1203
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaattac gaacgtaagg ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1204
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1204
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Tyr Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1205
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1205
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc tgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gggttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattc tgggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga tgttgttggc 1560
cagaaaccgg catcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1206
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1206
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Leu Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Asp Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1207
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1207
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcaccac tgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gggttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg catcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1208
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1208
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Thr Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Leu Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1209
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1209
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcaccga ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg ccatctgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gggttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtgcag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccga tttcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattgtgc cgtgtggaca t                                           1701

 
<210> 1210
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1210
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Glu Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Ala Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ile Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Val Pro Cys Gly His                                     
                565                                             

 
<210> 1211
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1211
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga tgttgttggc 1560
cagaaaccga tttcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1212
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1212
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Asp Val Val Gly Gln Lys Pro Ile Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Leu Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1213
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1213
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg ccatctgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg catcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1214
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1214
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Leu Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1215
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1215
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1216
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1216
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1217
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1217
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gggttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctaa attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaatctg gaacgtaagg ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtg tgattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1218
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1218
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Leu Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Val Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1219
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1219
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcaccac tgttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gtgttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc tgccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctaa attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaattac gaacgtaagg ttaatatggg tctgaaaggc 1260
actcagattg caggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccga tttcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1220
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1220
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Thr Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Leu Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Tyr Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Ala Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ile Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

 
<210> 1221
<211> 1701
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1221
atgaaacctt tgccacagga tcagagcaaa accagcagcc agcagtttag ctttaccggc 60
aatagcagcg caaacgtgat tattggtaat cagaaactga ccatcaatga tgttgtatgc 120
gttgcccgta atggcacccc ggttagcctg accaataatc cggatattct gcagggtatt 180
caggccagct gtgattttat caataatgca gttgaaagcg gtgaacacat ttatggtgtg 240
acctcgccgc caggtggtat ggcaaatgtt gtaattagcc gtgaacaggc cagcgaactg 300
cagatgaatg cgatttgggc ccttaaaacc ggcgcaggta ataaactgcc gctggcagat 360
gttcgtgcag caatgctgct gcgtgcaaat agccacatgc gtggtgcaag cggtattcgt 420
ctggaactga ttaaacgcat ggaaatcttt ctgaatgccg gggttacccc gtatgtttat 480
gaatttggta gcattggtgc cagcggtgat ctggtcccgg gatcgtatat taccggtagc 540
ctgattggtc tggacccgag ctttaaagtt gattttaatg gcaaagaaat ggacgcaccg 600
accgcactgc gtcagctgaa cctggagccg ctgaccctgc agccgaagga aggctgcgcg 660
atggtaaatg gcaccagcgt tatgaccggt attgcagcaa attgtgttta tgatacccag 720
attctgaccg cgattgcaat gggtgttcat gcactggata ttcaggcact gaatggtaca 780
aatcagagct ttcatccgtt tatccataac agcaaaccgc atccgggtca gctgtgggca 840
gcagatcaga tgattagcct gctggccggt agccagctgg ttcgtgatga actggatggt 900
aaacatgatt cgcgtgatgg cgaactgatt caggatcgtt atagcctgcg ttgtctgccg 960
cagtatctgg gtccgattgt tgatggtatt agccagattg ccaaacaaat cgaaattgag 1020
attaacagcg ttaccgataa cccgctgatt gatgttgata atcaggcaag ctattatggt 1080
ggtaattttc tgggtcagta tgttggtatg ggtatggatc atctgcgcta ttatatcggt 1140
ctgctggcaa aacatctgga tgttcagatt gcactgctga attcaccgga atttagcaat 1200
ggtctgcctc cgagtctggt gggtaataag gaacgtaaag ttaatatggg tctgaaaggc 1260
actcagattc tgggtaatag cattatgccg ctgctgacct tttatggtaa tagtattgca 1320
gatcgttttc cgacccatgc cgaacagttt aaccagaata ttaacagcca gggttatgcg 1380
agcgcaaccc tggcacgtcg tagcgttgat atttttcagg agtatgttgc cattgccctg 1440
atgtttggtg ttcaggcagt tgatctgcgt acctacaaaa aaaccggtca ttatgatgca 1500
cgtgcatgtc tgtcaccggc aaccgaacgt ctgtatagcg cagttcgtga agttgttggc 1560
cagaaaccgg cgtcagatcg tccgtatatt tggaatgata atgaacaggg tctggatgaa 1620
catattgcac gtattagtgc agatattgca gccggtggtc gcattgttca ggccgttcag 1680
gacattctgc cgtgtggaca t                                           1701

 
<210> 1222
<211> 567
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Phenylalanine Ammonia Lyase from Anabaena variabilis
 
<400> 1222
Met Lys Pro Leu Pro Gln Asp Gln Ser Lys Thr Ser Ser Gln Gln Phe 
1               5                   10                  15      
Ser Phe Thr Gly Asn Ser Ser Ala Asn Val Ile Ile Gly Asn Gln Lys 
            20                  25                  30          
Leu Thr Ile Asn Asp Val Val Cys Val Ala Arg Asn Gly Thr Pro Val 
        35                  40                  45              
Ser Leu Thr Asn Asn Pro Asp Ile Leu Gln Gly Ile Gln Ala Ser Cys 
    50                  55                  60                  
Asp Phe Ile Asn Asn Ala Val Glu Ser Gly Glu His Ile Tyr Gly Val 
65                  70                  75                  80  
Thr Ser Pro Pro Gly Gly Met Ala Asn Val Val Ile Ser Arg Glu Gln 
                85                  90                  95      
Ala Ser Glu Leu Gln Met Asn Ala Ile Trp Ala Leu Lys Thr Gly Ala 
            100                 105                 110         
Gly Asn Lys Leu Pro Leu Ala Asp Val Arg Ala Ala Met Leu Leu Arg 
        115                 120                 125             
Ala Asn Ser His Met Arg Gly Ala Ser Gly Ile Arg Leu Glu Leu Ile 
    130                 135                 140                 
Lys Arg Met Glu Ile Phe Leu Asn Ala Gly Val Thr Pro Tyr Val Tyr 
145                 150                 155                 160 
Glu Phe Gly Ser Ile Gly Ala Ser Gly Asp Leu Val Pro Gly Ser Tyr 
                165                 170                 175     
Ile Thr Gly Ser Leu Ile Gly Leu Asp Pro Ser Phe Lys Val Asp Phe 
            180                 185                 190         
Asn Gly Lys Glu Met Asp Ala Pro Thr Ala Leu Arg Gln Leu Asn Leu 
        195                 200                 205             
Glu Pro Leu Thr Leu Gln Pro Lys Glu Gly Cys Ala Met Val Asn Gly 
    210                 215                 220                 
Thr Ser Val Met Thr Gly Ile Ala Ala Asn Cys Val Tyr Asp Thr Gln 
225                 230                 235                 240 
Ile Leu Thr Ala Ile Ala Met Gly Val His Ala Leu Asp Ile Gln Ala 
                245                 250                 255     
Leu Asn Gly Thr Asn Gln Ser Phe His Pro Phe Ile His Asn Ser Lys 
            260                 265                 270         
Pro His Pro Gly Gln Leu Trp Ala Ala Asp Gln Met Ile Ser Leu Leu 
        275                 280                 285             
Ala Gly Ser Gln Leu Val Arg Asp Glu Leu Asp Gly Lys His Asp Ser 
    290                 295                 300                 
Arg Asp Gly Glu Leu Ile Gln Asp Arg Tyr Ser Leu Arg Cys Leu Pro 
305                 310                 315                 320 
Gln Tyr Leu Gly Pro Ile Val Asp Gly Ile Ser Gln Ile Ala Lys Gln 
                325                 330                 335     
Ile Glu Ile Glu Ile Asn Ser Val Thr Asp Asn Pro Leu Ile Asp Val 
            340                 345                 350         
Asp Asn Gln Ala Ser Tyr Tyr Gly Gly Asn Phe Leu Gly Gln Tyr Val 
        355                 360                 365             
Gly Met Gly Met Asp His Leu Arg Tyr Tyr Ile Gly Leu Leu Ala Lys 
    370                 375                 380                 
His Leu Asp Val Gln Ile Ala Leu Leu Asn Ser Pro Glu Phe Ser Asn 
385                 390                 395                 400 
Gly Leu Pro Pro Ser Leu Val Gly Asn Lys Glu Arg Lys Val Asn Met 
                405                 410                 415     
Gly Leu Lys Gly Thr Gln Ile Leu Gly Asn Ser Ile Met Pro Leu Leu 
            420                 425                 430         
Thr Phe Tyr Gly Asn Ser Ile Ala Asp Arg Phe Pro Thr His Ala Glu 
        435                 440                 445             
Gln Phe Asn Gln Asn Ile Asn Ser Gln Gly Tyr Ala Ser Ala Thr Leu 
    450                 455                 460                 
Ala Arg Arg Ser Val Asp Ile Phe Gln Glu Tyr Val Ala Ile Ala Leu 
465                 470                 475                 480 
Met Phe Gly Val Gln Ala Val Asp Leu Arg Thr Tyr Lys Lys Thr Gly 
                485                 490                 495     
His Tyr Asp Ala Arg Ala Cys Leu Ser Pro Ala Thr Glu Arg Leu Tyr 
            500                 505                 510         
Ser Ala Val Arg Glu Val Val Gly Gln Lys Pro Ala Ser Asp Arg Pro 
        515                 520                 525             
Tyr Ile Trp Asn Asp Asn Glu Gln Gly Leu Asp Glu His Ile Ala Arg 
    530                 535                 540                 
Ile Ser Ala Asp Ile Ala Ala Gly Gly Arg Ile Val Gln Ala Val Gln 
545                 550                 555                 560 
Asp Ile Leu Pro Cys Gly His                                     
                565                                             

