                         SEQUENCE LISTING

<110>  Keygene N.V.
 
<120>  Type-V CRISPR/nuclease system for genome editing in plant cells

<130>  P6077398PCT

<150>  US 62/697,021
<151>  2018-07-12

<150>  EP 18194078.4
<151>  2018-09-12

<160>  74    

<170>  PatentIn version 3.5

<210>  1
<211>  1263
<212>  PRT
<213>  Eubacterium rectale

<400>  1

Met Asn Asn Gly Thr Asn Asn Phe Gln Asn Phe Ile Gly Ile Ser Ser 
1               5                   10                  15      


Leu Gln Lys Thr Leu Arg Asn Ala Leu Ile Pro Thr Glu Thr Thr Gln 
            20                  25                  30          


Gln Phe Ile Val Lys Asn Gly Ile Ile Lys Glu Asp Glu Leu Arg Gly 
        35                  40                  45              


Glu Asn Arg Gln Ile Leu Lys Asp Ile Met Asp Asp Tyr Tyr Arg Gly 
    50                  55                  60                  


Phe Ile Ser Glu Thr Leu Ser Ser Ile Asp Asp Ile Asp Trp Thr Ser 
65                  70                  75                  80  


Leu Phe Glu Lys Met Glu Ile Gln Leu Lys Asn Gly Asp Asn Lys Asp 
                85                  90                  95      


Thr Leu Ile Lys Glu Gln Thr Glu Tyr Arg Lys Ala Ile His Lys Lys 
            100                 105                 110         


Phe Ala Asn Asp Asp Arg Phe Lys Asn Met Phe Ser Ala Lys Leu Ile 
        115                 120                 125             


Ser Asp Ile Leu Pro Glu Phe Val Ile His Asn Asn Asn Tyr Ser Ala 
    130                 135                 140                 


Ser Glu Lys Glu Glu Lys Thr Gln Val Ile Lys Leu Phe Ser Arg Phe 
145                 150                 155                 160 


Ala Thr Ser Phe Lys Asp Tyr Phe Lys Asn Arg Ala Asn Cys Phe Ser 
                165                 170                 175     


Ala Asp Asp Ile Ser Ser Ser Ser Cys His Arg Ile Val Asn Asp Asn 
            180                 185                 190         


Ala Glu Ile Phe Phe Ser Asn Ala Leu Val Tyr Arg Arg Ile Val Lys 
        195                 200                 205             


Ser Leu Ser Asn Asp Asp Ile Asn Lys Ile Ser Gly Asp Met Lys Asp 
    210                 215                 220                 


Ser Leu Lys Glu Met Ser Leu Glu Glu Ile Tyr Ser Tyr Glu Lys Tyr 
225                 230                 235                 240 


Gly Glu Phe Ile Thr Gln Glu Gly Ile Ser Phe Tyr Asn Asp Ile Cys 
                245                 250                 255     


Gly Lys Val Asn Ser Phe Met Asn Leu Tyr Cys Gln Lys Asn Lys Glu 
            260                 265                 270         


Asn Lys Asn Leu Tyr Lys Leu Gln Lys Leu His Lys Gln Ile Leu Cys 
        275                 280                 285             


Ile Ala Asp Thr Ser Tyr Glu Val Pro Tyr Lys Phe Glu Ser Asp Glu 
    290                 295                 300                 


Glu Val Tyr Gln Ser Val Asn Gly Phe Leu Asp Asn Ile Ser Ser Lys 
305                 310                 315                 320 


His Ile Val Glu Arg Leu Arg Lys Ile Gly Asp Asn Tyr Asn Gly Tyr 
                325                 330                 335     


Asn Leu Asp Lys Ile Tyr Ile Val Ser Lys Phe Tyr Glu Ser Val Ser 
            340                 345                 350         


Gln Lys Thr Tyr Arg Asp Trp Glu Thr Ile Asn Thr Ala Leu Glu Ile 
        355                 360                 365             


His Tyr Asn Asn Ile Leu Pro Gly Asn Gly Lys Ser Lys Ala Asp Lys 
    370                 375                 380                 


Val Lys Lys Ala Val Lys Asn Asp Leu Gln Lys Ser Ile Thr Glu Ile 
385                 390                 395                 400 


Asn Glu Leu Val Ser Asn Tyr Lys Leu Cys Ser Asp Asp Asn Ile Lys 
                405                 410                 415     


Ala Glu Thr Tyr Ile His Glu Ile Ser His Ile Leu Asn Asn Phe Glu 
            420                 425                 430         


Ala Gln Glu Leu Lys Tyr Asn Pro Glu Ile His Leu Val Glu Ser Glu 
        435                 440                 445             


Leu Lys Ala Ser Glu Leu Lys Asn Val Leu Asp Val Ile Met Asn Ala 
    450                 455                 460                 


Phe His Trp Cys Ser Val Phe Met Thr Glu Glu Leu Val Asp Lys Asp 
465                 470                 475                 480 


Asn Asn Phe Tyr Ala Glu Leu Glu Glu Ile Tyr Asp Glu Ile Tyr Pro 
                485                 490                 495     


Val Ile Ser Leu Tyr Asn Leu Val Arg Asn Tyr Val Thr Gln Lys Pro 
            500                 505                 510         


Tyr Ser Thr Lys Lys Ile Lys Leu Asn Phe Gly Ile Pro Thr Leu Ala 
        515                 520                 525             


Asp Gly Trp Ser Lys Ser Lys Glu Tyr Ser Asn Asn Ala Ile Ile Leu 
    530                 535                 540                 


Met Arg Asp Asn Leu Tyr Tyr Leu Gly Ile Phe Asn Ala Lys Asn Lys 
545                 550                 555                 560 


Pro Asp Lys Lys Ile Ile Glu Gly Asn Thr Ser Glu Asn Lys Gly Asp 
                565                 570                 575     


Tyr Lys Lys Met Ile Tyr Asn Leu Leu Pro Gly Pro Asn Lys Met Ile 
            580                 585                 590         


Pro Lys Val Phe Leu Ser Ser Lys Thr Gly Val Glu Thr Tyr Lys Pro 
        595                 600                 605             


Ser Ala Tyr Ile Leu Glu Gly Tyr Lys Gln Asn Lys His Ile Lys Ser 
    610                 615                 620                 


Ser Lys Asp Phe Asp Ile Thr Phe Cys His Asp Leu Ile Asp Tyr Phe 
625                 630                 635                 640 


Lys Asn Cys Ile Ala Ile His Pro Glu Trp Lys Asn Phe Gly Phe Asp 
                645                 650                 655     


Phe Ser Asp Thr Ser Thr Tyr Glu Asp Ile Ser Gly Phe Tyr Arg Glu 
            660                 665                 670         


Val Glu Leu Gln Gly Tyr Lys Ile Asp Trp Thr Tyr Ile Ser Glu Lys 
        675                 680                 685             


Asp Ile Asp Leu Leu Gln Glu Lys Gly Gln Leu Tyr Leu Phe Gln Ile 
    690                 695                 700                 


Tyr Asn Lys Asp Phe Ser Lys Lys Ser Thr Gly Asn Asp Asn Leu His 
705                 710                 715                 720 


Thr Met Tyr Leu Lys Asn Leu Phe Ser Glu Glu Asn Leu Lys Asp Ile 
                725                 730                 735     


Val Leu Lys Leu Asn Gly Glu Ala Glu Ile Phe Phe Arg Lys Ser Ser 
            740                 745                 750         


Ile Lys Asn Pro Ile Ile His Lys Lys Gly Ser Ile Leu Val Asn Arg 
        755                 760                 765             


Thr Tyr Glu Ala Glu Glu Lys Asp Gln Phe Gly Asn Ile Gln Ile Val 
    770                 775                 780                 


Arg Lys Asn Ile Pro Glu Asn Ile Tyr Gln Glu Leu Tyr Lys Tyr Phe 
785                 790                 795                 800 


Asn Asp Lys Ser Asp Lys Glu Leu Ser Asp Glu Ala Ala Lys Leu Lys 
                805                 810                 815     


Asn Val Val Gly His His Glu Ala Ala Thr Asn Ile Val Lys Asp Tyr 
            820                 825                 830         


Arg Tyr Thr Tyr Asp Lys Tyr Phe Leu His Met Pro Ile Thr Ile Asn 
        835                 840                 845             


Phe Lys Ala Asn Lys Thr Gly Phe Ile Asn Asp Arg Ile Leu Gln Tyr 
    850                 855                 860                 


Ile Ala Lys Glu Lys Asp Leu His Val Ile Gly Ile Asp Arg Gly Glu 
865                 870                 875                 880 


Arg Asn Leu Ile Tyr Val Ser Val Ile Asp Thr Cys Gly Asn Ile Val 
                885                 890                 895     


Glu Gln Lys Ser Phe Asn Ile Val Asn Gly Tyr Asp Tyr Gln Ile Lys 
            900                 905                 910         


Leu Lys Gln Gln Glu Gly Ala Arg Gln Ile Ala Arg Lys Glu Trp Lys 
        915                 920                 925             


Glu Ile Gly Lys Ile Lys Glu Ile Lys Glu Gly Tyr Leu Ser Leu Val 
    930                 935                 940                 


Ile His Glu Ile Ser Lys Met Val Ile Lys Tyr Asn Ala Ile Ile Ala 
945                 950                 955                 960 


Met Glu Asp Leu Ser Tyr Gly Phe Lys Lys Gly Arg Phe Lys Val Glu 
                965                 970                 975     


Arg Gln Val Tyr Gln Lys Phe Glu Thr Met Leu Ile Asn Lys Leu Asn 
            980                 985                 990         


Tyr Leu Val Phe Lys Asp Ile Ser  Ile Thr Glu Asn Gly  Gly Leu Leu 
        995                 1000                 1005             


Lys Gly  Tyr Gln Leu Thr Tyr  Ile Pro Asp Lys Leu  Lys Asn Val 
    1010                 1015                 1020             


Gly His  Gln Cys Gly Cys Ile  Phe Tyr Val Pro Ala  Ala Tyr Thr 
    1025                 1030                 1035             


Ser Lys  Ile Asp Pro Thr Thr  Gly Phe Val Asn Ile  Phe Lys Phe 
    1040                 1045                 1050             


Lys Asp  Leu Thr Val Asp Ala  Lys Arg Glu Phe Ile  Lys Lys Phe 
    1055                 1060                 1065             


Asp Ser  Ile Arg Tyr Asp Ser  Glu Lys Asn Leu Phe  Cys Phe Thr 
    1070                 1075                 1080             


Phe Asp  Tyr Asn Asn Phe Ile  Thr Gln Asn Thr Val  Met Ser Lys 
    1085                 1090                 1095             


Ser Ser  Trp Ser Val Tyr Thr  Tyr Gly Val Arg Ile  Lys Arg Arg 
    1100                 1105                 1110             


Phe Val  Asn Gly Arg Phe Ser  Asn Glu Ser Asp Thr  Ile Asp Ile 
    1115                 1120                 1125             


Thr Lys  Asp Met Glu Lys Thr  Leu Glu Met Thr Asp  Ile Asn Trp 
    1130                 1135                 1140             


Arg Asp  Gly His Asp Leu Arg  Gln Asp Ile Ile Asp  Tyr Glu Ile 
    1145                 1150                 1155             


Val Gln  His Ile Phe Glu Ile  Phe Arg Leu Thr Val  Gln Met Arg 
    1160                 1165                 1170             


Asn Ser  Leu Ser Glu Leu Glu  Asp Arg Asp Tyr Asp  Arg Leu Ile 
    1175                 1180                 1185             


Ser Pro  Val Leu Asn Glu Asn  Asn Ile Phe Tyr Asp  Ser Ala Lys 
    1190                 1195                 1200             


Ala Gly  Asp Ala Leu Pro Lys  Asp Ala Asp Ala Asn  Gly Ala Tyr 
    1205                 1210                 1215             


Cys Ile  Ala Leu Lys Gly Leu  Tyr Glu Ile Lys Gln  Ile Thr Glu 
    1220                 1225                 1230             


Asn Trp  Lys Glu Asp Gly Lys  Phe Ser Arg Asp Lys  Leu Lys Ile 
    1235                 1240                 1245             


Ser Asn  Lys Asp Trp Phe Asp  Phe Ile Gln Asn Lys  Arg Tyr Leu 
    1250                 1255                 1260             


<210>  2
<211>  3789
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MAD7 coding sequence codon optimized for S. lycopersicum

<400>  2
atgaacaacg ggaccaacaa cttccagaac ttcatcggga tcagctccct ccaaaagacc       60

cttaggaacg ctcttatccc aaccgagact acccagcagt tcatcgtgaa gaacgggatc      120

atcaaagagg acgagcttag gggagagaac aggcagatcc tcaaggatat catggacgac      180

tactacaggg gcttcatctc cgagactctg tcctccatcg atgatatcga ctggacctcc      240

ttgttcgaga agatggaaat ccagctcaag aacggggaca acaaggacac ccttatcaaa      300

gagcagaccg agtaccgtaa ggccatccac aaaaagttcg ctaacgacga caggttcaag      360

aatatgttct ccgccaagct catcagcgac atccttccag agttcgtgat ccacaacaac      420

aactactccg cctccgagaa agaggaaaag acccaggtca tcaagctgtt ctccaggttc      480

gctacctcct tcaaggacta cttcaagaac agggccaact gcttctccgc cgacgatatc      540

tcttcatctt cttgccacag gatcgtgaac gacaacgccg agatcttctt ctccaacgct      600

cttgtgtaca ggcgtatcgt gaagtccctc tccaacgatg acatcaacaa gatctccggg      660

gacatgaagg acagcctcaa agagatgtcc ctcgaagaga tctactccta cgagaagtac      720

ggggagttca tcacccaaga gggcatcagc ttctacaacg acatctgcgg aaaggtgaac      780

tccttcatga acctctactg ccagaagaac aaagagaaca agaacctgta caagctccag      840

aagctccaca agcagatcct gtgcattgct gacactagct acgaggtgcc atacaagttc      900

gagtccgatg aagaggtgta ccagtccgtg aatggcttcc tcgacaacat ctcctctaag      960

cacatcgtcg agaggcttag gaagatcggc gataactaca acgggtacaa cctggacaag     1020

atctacatcg tgtccaagtt ctacgagtcc gtgagccaga aaacctaccg tgattgggag     1080

actatcaaca ccgctctcga gattcactac aacaacattc tccccggcaa cgggaaatcc     1140

aaggctgata aggttaagaa ggccgtcaag aacgacctcc agaagtctat cactgagatc     1200

aacgagctgg tgtccaacta caagctctgc tccgatgaca acatcaaggc cgagacttac     1260

atccacgaga tctcccacat cctgaacaac ttcgaggccc aagagctgaa gtacaaccca     1320

gagattcacc tcgtcgagtc tgagcttaag gcctccgagc ttaagaacgt gctcgacgtt     1380

atcatgaacg ccttccattg gtgctccgtg ttcatgactg aagagttggt ggacaaggat     1440

aacaacttct acgccgagct ggaagaaatc tacgacgaga tctaccccgt gatctccctc     1500

tacaacctcg tgaggaatta cgtgacccag aagccatact ccaccaagaa gatcaagctc     1560

aacttcggga tcccaacctt ggctgatgga tggtccaagt ccaaagagta ctccaacaac     1620

gccatcatcc tgatgaggga caacctgtac tacctcggga tcttcaacgc caagaacaag     1680

cccgacaaga agattatcga ggggaacacc tctgagaaca agggcgacta caaaaagatg     1740

atctacaact tgctcccagg gcctaacaag atgatcccca aggtgttcct cagctccaag     1800

actggtgttg agacatacaa gccctccgcc tacattctcg agggatacaa gcaaaacaag     1860

cacatcaagt cctccaagga cttcgatatc accttctgcc acgacctcat cgattatttc     1920

aagaactgca ttgccattca tccagagtgg aagaacttcg ggttcgactt ctccgatacc     1980

tccacctacg aggatatctc cggattctac cgtgaggttg agcttcaggg gtacaagatc     2040

gattggacct acatcagcga gaaggacatc gacctgttgc aagagaaggg gcagctttac     2100

ctcttccaaa tctacaacaa agacttctcc aaaaagtcca ccggcaacga caacctccac     2160

accatgtacc tcaagaacct cttcagcgaa gagaacctca aggacattgt gctcaagctg     2220

aatggcgagg ctgagatttt ctttaggaag tcctctatca agaaccccat catccacaag     2280

aagggctcca tcctcgttaa caggacttac gaggctgaag agaaggacca gttcgggaac     2340

attcagatcg tgcgtaagaa catccccgag aacatctacc aagagcttta caagtacttc     2400

aacgacaagt ccgacaaaga gctgtctgac gaggctgcca agttgaagaa tgtggtggga     2460

catcatgagg ccgctaccaa cattgtgaag gactacaggt acacttacga caagtatttc     2520

ttgcacatgc ccatcaccat caacttcaag gccaacaaga ccggcttcat caacgacagg     2580

atcctccagt acattgccaa agaaaaggac ctccacgtga tcgggattga taggggtgag     2640

cgaaacctca tctacgtgtc agtgatcgat acctgcggga acatcgttga gcagaagtcc     2700

ttcaacatcg ttaacgggta cgactaccag attaagctca agcagcaaga gggtgctaga     2760

cagatcgcta ggaaagagtg gaaagagatc gggaaaatca aagagatcaa agaaggctac     2820

ctctccttgg tgattcacga gatcagcaag atggtgatca agtacaacgc tattatcgcc     2880

atggaagatc tcagctacgg cttcaagaag gggcgtttta aggttgagag gcaggtctac     2940

caaaagttcg agactatgct gatcaacaag ctgaactacc tggtgtttaa ggacatctcc     3000

attaccgaga acggcgggct tcttaaggga taccagctca cttacattcc cgacaaactg     3060

aagaacgtgg gtcaccaatg cggctgcatt ttctatgttc cagctgccta cacctccaag     3120

atcgacccaa ctactggatt cgtgaacatc ttcaagttca aggacctcac cgtggacgct     3180

aagagggaat tcatcaagaa gttcgactcc atcaggtacg actccgagaa gaaccttttc     3240

tgcttcacct tcgattacaa caactttatc acccagaaca ccgtgatgtc caagagcagc     3300

tggtcagtgt acacatacgg tgtgaggatc aagcgacgtt tcgtgaacgg aaggttcagc     3360

aacgagagcg atacaatcga catcactaag gacatggaaa agactcttga gatgaccgac     3420

atcaactggc gtgatggtca tgatctcagg caggacatta tcgactacga gatcgtgcag     3480

cacatcttcg aaatcttcag gctcactgtg cagatgagga actcccttag tgagcttgag     3540

gatagggact acgacaggct tatctcccca gtgcttaacg agaacaacat cttctacgac     3600

agcgctaagg ctggggatgc tttgccaaaa gatgctgatg ctaacggggc ttactgtatc     3660

gctttgaagg ggctgtacga gattaagcag atcaccgaga actggaaaga ggatggcaag     3720

ttctcccgtg acaagctcaa gatctctaac aaggactggt tcgatttcat tcagaacaag     3780

cgttacctc                                                             3789


<210>  3
<211>  1274
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MAD7-NLS

<400>  3

Met Asn Asn Gly Thr Asn Asn Phe Gln Asn Phe Ile Gly Ile Ser Ser 
1               5                   10                  15      


Leu Gln Lys Thr Leu Arg Asn Ala Leu Ile Pro Thr Glu Thr Thr Gln 
            20                  25                  30          


Gln Phe Ile Val Lys Asn Gly Ile Ile Lys Glu Asp Glu Leu Arg Gly 
        35                  40                  45              


Glu Asn Arg Gln Ile Leu Lys Asp Ile Met Asp Asp Tyr Tyr Arg Gly 
    50                  55                  60                  


Phe Ile Ser Glu Thr Leu Ser Ser Ile Asp Asp Ile Asp Trp Thr Ser 
65                  70                  75                  80  


Leu Phe Glu Lys Met Glu Ile Gln Leu Lys Asn Gly Asp Asn Lys Asp 
                85                  90                  95      


Thr Leu Ile Lys Glu Gln Thr Glu Tyr Arg Lys Ala Ile His Lys Lys 
            100                 105                 110         


Phe Ala Asn Asp Asp Arg Phe Lys Asn Met Phe Ser Ala Lys Leu Ile 
        115                 120                 125             


Ser Asp Ile Leu Pro Glu Phe Val Ile His Asn Asn Asn Tyr Ser Ala 
    130                 135                 140                 


Ser Glu Lys Glu Glu Lys Thr Gln Val Ile Lys Leu Phe Ser Arg Phe 
145                 150                 155                 160 


Ala Thr Ser Phe Lys Asp Tyr Phe Lys Asn Arg Ala Asn Cys Phe Ser 
                165                 170                 175     


Ala Asp Asp Ile Ser Ser Ser Ser Cys His Arg Ile Val Asn Asp Asn 
            180                 185                 190         


Ala Glu Ile Phe Phe Ser Asn Ala Leu Val Tyr Arg Arg Ile Val Lys 
        195                 200                 205             


Ser Leu Ser Asn Asp Asp Ile Asn Lys Ile Ser Gly Asp Met Lys Asp 
    210                 215                 220                 


Ser Leu Lys Glu Met Ser Leu Glu Glu Ile Tyr Ser Tyr Glu Lys Tyr 
225                 230                 235                 240 


Gly Glu Phe Ile Thr Gln Glu Gly Ile Ser Phe Tyr Asn Asp Ile Cys 
                245                 250                 255     


Gly Lys Val Asn Ser Phe Met Asn Leu Tyr Cys Gln Lys Asn Lys Glu 
            260                 265                 270         


Asn Lys Asn Leu Tyr Lys Leu Gln Lys Leu His Lys Gln Ile Leu Cys 
        275                 280                 285             


Ile Ala Asp Thr Ser Tyr Glu Val Pro Tyr Lys Phe Glu Ser Asp Glu 
    290                 295                 300                 


Glu Val Tyr Gln Ser Val Asn Gly Phe Leu Asp Asn Ile Ser Ser Lys 
305                 310                 315                 320 


His Ile Val Glu Arg Leu Arg Lys Ile Gly Asp Asn Tyr Asn Gly Tyr 
                325                 330                 335     


Asn Leu Asp Lys Ile Tyr Ile Val Ser Lys Phe Tyr Glu Ser Val Ser 
            340                 345                 350         


Gln Lys Thr Tyr Arg Asp Trp Glu Thr Ile Asn Thr Ala Leu Glu Ile 
        355                 360                 365             


His Tyr Asn Asn Ile Leu Pro Gly Asn Gly Lys Ser Lys Ala Asp Lys 
    370                 375                 380                 


Val Lys Lys Ala Val Lys Asn Asp Leu Gln Lys Ser Ile Thr Glu Ile 
385                 390                 395                 400 


Asn Glu Leu Val Ser Asn Tyr Lys Leu Cys Ser Asp Asp Asn Ile Lys 
                405                 410                 415     


Ala Glu Thr Tyr Ile His Glu Ile Ser His Ile Leu Asn Asn Phe Glu 
            420                 425                 430         


Ala Gln Glu Leu Lys Tyr Asn Pro Glu Ile His Leu Val Glu Ser Glu 
        435                 440                 445             


Leu Lys Ala Ser Glu Leu Lys Asn Val Leu Asp Val Ile Met Asn Ala 
    450                 455                 460                 


Phe His Trp Cys Ser Val Phe Met Thr Glu Glu Leu Val Asp Lys Asp 
465                 470                 475                 480 


Asn Asn Phe Tyr Ala Glu Leu Glu Glu Ile Tyr Asp Glu Ile Tyr Pro 
                485                 490                 495     


Val Ile Ser Leu Tyr Asn Leu Val Arg Asn Tyr Val Thr Gln Lys Pro 
            500                 505                 510         


Tyr Ser Thr Lys Lys Ile Lys Leu Asn Phe Gly Ile Pro Thr Leu Ala 
        515                 520                 525             


Asp Gly Trp Ser Lys Ser Lys Glu Tyr Ser Asn Asn Ala Ile Ile Leu 
    530                 535                 540                 


Met Arg Asp Asn Leu Tyr Tyr Leu Gly Ile Phe Asn Ala Lys Asn Lys 
545                 550                 555                 560 


Pro Asp Lys Lys Ile Ile Glu Gly Asn Thr Ser Glu Asn Lys Gly Asp 
                565                 570                 575     


Tyr Lys Lys Met Ile Tyr Asn Leu Leu Pro Gly Pro Asn Lys Met Ile 
            580                 585                 590         


Pro Lys Val Phe Leu Ser Ser Lys Thr Gly Val Glu Thr Tyr Lys Pro 
        595                 600                 605             


Ser Ala Tyr Ile Leu Glu Gly Tyr Lys Gln Asn Lys His Ile Lys Ser 
    610                 615                 620                 


Ser Lys Asp Phe Asp Ile Thr Phe Cys His Asp Leu Ile Asp Tyr Phe 
625                 630                 635                 640 


Lys Asn Cys Ile Ala Ile His Pro Glu Trp Lys Asn Phe Gly Phe Asp 
                645                 650                 655     


Phe Ser Asp Thr Ser Thr Tyr Glu Asp Ile Ser Gly Phe Tyr Arg Glu 
            660                 665                 670         


Val Glu Leu Gln Gly Tyr Lys Ile Asp Trp Thr Tyr Ile Ser Glu Lys 
        675                 680                 685             


Asp Ile Asp Leu Leu Gln Glu Lys Gly Gln Leu Tyr Leu Phe Gln Ile 
    690                 695                 700                 


Tyr Asn Lys Asp Phe Ser Lys Lys Ser Thr Gly Asn Asp Asn Leu His 
705                 710                 715                 720 


Thr Met Tyr Leu Lys Asn Leu Phe Ser Glu Glu Asn Leu Lys Asp Ile 
                725                 730                 735     


Val Leu Lys Leu Asn Gly Glu Ala Glu Ile Phe Phe Arg Lys Ser Ser 
            740                 745                 750         


Ile Lys Asn Pro Ile Ile His Lys Lys Gly Ser Ile Leu Val Asn Arg 
        755                 760                 765             


Thr Tyr Glu Ala Glu Glu Lys Asp Gln Phe Gly Asn Ile Gln Ile Val 
    770                 775                 780                 


Arg Lys Asn Ile Pro Glu Asn Ile Tyr Gln Glu Leu Tyr Lys Tyr Phe 
785                 790                 795                 800 


Asn Asp Lys Ser Asp Lys Glu Leu Ser Asp Glu Ala Ala Lys Leu Lys 
                805                 810                 815     


Asn Val Val Gly His His Glu Ala Ala Thr Asn Ile Val Lys Asp Tyr 
            820                 825                 830         


Arg Tyr Thr Tyr Asp Lys Tyr Phe Leu His Met Pro Ile Thr Ile Asn 
        835                 840                 845             


Phe Lys Ala Asn Lys Thr Gly Phe Ile Asn Asp Arg Ile Leu Gln Tyr 
    850                 855                 860                 


Ile Ala Lys Glu Lys Asp Leu His Val Ile Gly Ile Asp Arg Gly Glu 
865                 870                 875                 880 


Arg Asn Leu Ile Tyr Val Ser Val Ile Asp Thr Cys Gly Asn Ile Val 
                885                 890                 895     


Glu Gln Lys Ser Phe Asn Ile Val Asn Gly Tyr Asp Tyr Gln Ile Lys 
            900                 905                 910         


Leu Lys Gln Gln Glu Gly Ala Arg Gln Ile Ala Arg Lys Glu Trp Lys 
        915                 920                 925             


Glu Ile Gly Lys Ile Lys Glu Ile Lys Glu Gly Tyr Leu Ser Leu Val 
    930                 935                 940                 


Ile His Glu Ile Ser Lys Met Val Ile Lys Tyr Asn Ala Ile Ile Ala 
945                 950                 955                 960 


Met Glu Asp Leu Ser Tyr Gly Phe Lys Lys Gly Arg Phe Lys Val Glu 
                965                 970                 975     


Arg Gln Val Tyr Gln Lys Phe Glu Thr Met Leu Ile Asn Lys Leu Asn 
            980                 985                 990         


Tyr Leu Val Phe Lys Asp Ile Ser  Ile Thr Glu Asn Gly  Gly Leu Leu 
        995                 1000                 1005             


Lys Gly  Tyr Gln Leu Thr Tyr  Ile Pro Asp Lys Leu  Lys Asn Val 
    1010                 1015                 1020             


Gly His  Gln Cys Gly Cys Ile  Phe Tyr Val Pro Ala  Ala Tyr Thr 
    1025                 1030                 1035             


Ser Lys  Ile Asp Pro Thr Thr  Gly Phe Val Asn Ile  Phe Lys Phe 
    1040                 1045                 1050             


Lys Asp  Leu Thr Val Asp Ala  Lys Arg Glu Phe Ile  Lys Lys Phe 
    1055                 1060                 1065             


Asp Ser  Ile Arg Tyr Asp Ser  Glu Lys Asn Leu Phe  Cys Phe Thr 
    1070                 1075                 1080             


Phe Asp  Tyr Asn Asn Phe Ile  Thr Gln Asn Thr Val  Met Ser Lys 
    1085                 1090                 1095             


Ser Ser  Trp Ser Val Tyr Thr  Tyr Gly Val Arg Ile  Lys Arg Arg 
    1100                 1105                 1110             


Phe Val  Asn Gly Arg Phe Ser  Asn Glu Ser Asp Thr  Ile Asp Ile 
    1115                 1120                 1125             


Thr Lys  Asp Met Glu Lys Thr  Leu Glu Met Thr Asp  Ile Asn Trp 
    1130                 1135                 1140             


Arg Asp  Gly His Asp Leu Arg  Gln Asp Ile Ile Asp  Tyr Glu Ile 
    1145                 1150                 1155             


Val Gln  His Ile Phe Glu Ile  Phe Arg Leu Thr Val  Gln Met Arg 
    1160                 1165                 1170             


Asn Ser  Leu Ser Glu Leu Glu  Asp Arg Asp Tyr Asp  Arg Leu Ile 
    1175                 1180                 1185             


Ser Pro  Val Leu Asn Glu Asn  Asn Ile Phe Tyr Asp  Ser Ala Lys 
    1190                 1195                 1200             


Ala Gly  Asp Ala Leu Pro Lys  Asp Ala Asp Ala Asn  Gly Ala Tyr 
    1205                 1210                 1215             


Cys Ile  Ala Leu Lys Gly Leu  Tyr Glu Ile Lys Gln  Ile Thr Glu 
    1220                 1225                 1230             


Asn Trp  Lys Glu Asp Gly Lys  Phe Ser Arg Asp Lys  Leu Lys Ile 
    1235                 1240                 1245             


Ser Asn  Lys Asp Trp Phe Asp  Phe Ile Gln Asn Lys  Arg Tyr Leu 
    1250                 1255                 1260             


Ser Gly  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1265                 1270                 


<210>  4
<211>  3825
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MAD7-NLS coding sequence codon optimized for S. lycopersicum

<400>  4
atgaacaacg ggaccaacaa cttccagaac ttcatcggga tcagctccct ccaaaagacc       60

cttaggaacg ctcttatccc aaccgagact acccagcagt tcatcgtgaa gaacgggatc      120

atcaaagagg acgagcttag gggagagaac aggcagatcc tcaaggatat catggacgac      180

tactacaggg gcttcatctc cgagactctg tcctccatcg atgatatcga ctggacctcc      240

ttgttcgaga agatggaaat ccagctcaag aacggggaca acaaggacac ccttatcaaa      300

gagcagaccg agtaccgtaa ggccatccac aaaaagttcg ctaacgacga caggttcaag      360

aatatgttct ccgccaagct catcagcgac atccttccag agttcgtgat ccacaacaac      420

aactactccg cctccgagaa agaggaaaag acccaggtca tcaagctgtt ctccaggttc      480

gctacctcct tcaaggacta cttcaagaac agggccaact gcttctccgc cgacgatatc      540

tcttcatctt cttgccacag gatcgtgaac gacaacgccg agatcttctt ctccaacgct      600

cttgtgtaca ggcgtatcgt gaagtccctc tccaacgatg acatcaacaa gatctccggg      660

gacatgaagg acagcctcaa agagatgtcc ctcgaagaga tctactccta cgagaagtac      720

ggggagttca tcacccaaga gggcatcagc ttctacaacg acatctgcgg aaaggtgaac      780

tccttcatga acctctactg ccagaagaac aaagagaaca agaacctgta caagctccag      840

aagctccaca agcagatcct gtgcattgct gacactagct acgaggtgcc atacaagttc      900

gagtccgatg aagaggtgta ccagtccgtg aatggcttcc tcgacaacat ctcctctaag      960

cacatcgtcg agaggcttag gaagatcggc gataactaca acgggtacaa cctggacaag     1020

atctacatcg tgtccaagtt ctacgagtcc gtgagccaga aaacctaccg tgattgggag     1080

actatcaaca ccgctctcga gattcactac aacaacattc tccccggcaa cgggaaatcc     1140

aaggctgata aggttaagaa ggccgtcaag aacgacctcc agaagtctat cactgagatc     1200

aacgagctgg tgtccaacta caagctctgc tccgatgaca acatcaaggc cgagacttac     1260

atccacgaga tctcccacat cctgaacaac ttcgaggccc aagagctgaa gtacaaccca     1320

gagattcacc tcgtcgagtc tgagcttaag gcctccgagc ttaagaacgt gctcgacgtt     1380

atcatgaacg ccttccattg gtgctccgtg ttcatgactg aagagttggt ggacaaggat     1440

aacaacttct acgccgagct ggaagaaatc tacgacgaga tctaccccgt gatctccctc     1500

tacaacctcg tgaggaatta cgtgacccag aagccatact ccaccaagaa gatcaagctc     1560

aacttcggga tcccaacctt ggctgatgga tggtccaagt ccaaagagta ctccaacaac     1620

gccatcatcc tgatgaggga caacctgtac tacctcggga tcttcaacgc caagaacaag     1680

cccgacaaga agattatcga ggggaacacc tctgagaaca agggcgacta caaaaagatg     1740

atctacaact tgctcccagg gcctaacaag atgatcccca aggtgttcct cagctccaag     1800

actggtgttg agacatacaa gccctccgcc tacattctcg agggatacaa gcaaaacaag     1860

cacatcaagt cctccaagga cttcgatatc accttctgcc acgacctcat cgattatttc     1920

aagaactgca ttgccattca tccagagtgg aagaacttcg ggttcgactt ctccgatacc     1980

tccacctacg aggatatctc cggattctac cgtgaggttg agcttcaggg gtacaagatc     2040

gattggacct acatcagcga gaaggacatc gacctgttgc aagagaaggg gcagctttac     2100

ctcttccaaa tctacaacaa agacttctcc aaaaagtcca ccggcaacga caacctccac     2160

accatgtacc tcaagaacct cttcagcgaa gagaacctca aggacattgt gctcaagctg     2220

aatggcgagg ctgagatttt ctttaggaag tcctctatca agaaccccat catccacaag     2280

aagggctcca tcctcgttaa caggacttac gaggctgaag agaaggacca gttcgggaac     2340

attcagatcg tgcgtaagaa catccccgag aacatctacc aagagcttta caagtacttc     2400

aacgacaagt ccgacaaaga gctgtctgac gaggctgcca agttgaagaa tgtggtggga     2460

catcatgagg ccgctaccaa cattgtgaag gactacaggt acacttacga caagtatttc     2520

ttgcacatgc ccatcaccat caacttcaag gccaacaaga ccggcttcat caacgacagg     2580

atcctccagt acattgccaa agaaaaggac ctccacgtga tcgggattga taggggtgag     2640

cgaaacctca tctacgtgtc agtgatcgat acctgcggga acatcgttga gcagaagtcc     2700

ttcaacatcg ttaacgggta cgactaccag attaagctca agcagcaaga gggtgctaga     2760

cagatcgcta ggaaagagtg gaaagagatc gggaaaatca aagagatcaa agaaggctac     2820

ctctccttgg tgattcacga gatcagcaag atggtgatca agtacaacgc tattatcgcc     2880

atggaagatc tcagctacgg cttcaagaag gggcgtttta aggttgagag gcaggtctac     2940

caaaagttcg agactatgct gatcaacaag ctgaactacc tggtgtttaa ggacatctcc     3000

attaccgaga acggcgggct tcttaaggga taccagctca cttacattcc cgacaaactg     3060

aagaacgtgg gtcaccaatg cggctgcatt ttctatgttc cagctgccta cacctccaag     3120

atcgacccaa ctactggatt cgtgaacatc ttcaagttca aggacctcac cgtggacgct     3180

aagagggaat tcatcaagaa gttcgactcc atcaggtacg actccgagaa gaaccttttc     3240

tgcttcacct tcgattacaa caactttatc acccagaaca ccgtgatgtc caagagcagc     3300

tggtcagtgt acacatacgg tgtgaggatc aagcgacgtt tcgtgaacgg aaggttcagc     3360

aacgagagcg atacaatcga catcactaag gacatggaaa agactcttga gatgaccgac     3420

atcaactggc gtgatggtca tgatctcagg caggacatta tcgactacga gatcgtgcag     3480

cacatcttcg aaatcttcag gctcactgtg cagatgagga actcccttag tgagcttgag     3540

gatagggact acgacaggct tatctcccca gtgcttaacg agaacaacat cttctacgac     3600

agcgctaagg ctggggatgc tttgccaaaa gatgctgatg ctaacggggc ttactgtatc     3660

gctttgaagg ggctgtacga gattaagcag atcaccgaga actggaaaga ggatggcaag     3720

ttctcccgtg acaagctcaa gatctctaac aaggactggt tcgatttcat tcagaacaag     3780

cgttacctca gcggcgggtc acctaagaag aagagaaagg tttga                     3825


<210>  5
<211>  1326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AsCpf1-NLS

<400>  5

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn Gly 
    1295                 1300                 1305             


Arg Gly  Ser His His His His  His His Lys Leu Pro  Lys Lys Lys 
    1310                 1315                 1320             


Arg Lys  Val 
    1325     


<210>  6
<211>  3981
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AsCpf1-NLS coding sequence codon optimized for S. lycopersicum

<400>  6
atgactcagt tcgagggatt cactaacctt taccaggtgt caaagactct taggttcgag       60

cttatcccac agggaaagac tttgaagcac atccaagagc agggattcat cgaagaggat      120

aaggctagga acgatcacta caaagagctt aagccaatca tcgataggat ctacaagact      180

tacgctgatc agtgccttca gcttgtgcag cttgattggg agaacctttc tgctgctatc      240

gattcttata ggaaagaaaa gactgaagag actaggaacg ctcttatcga ggaacaggct      300

acttacagaa acgctatcca cgattacttc atcggaagga ctgataactt gactgatgct      360

atcaacaaga ggcacgctga gatctataag ggacttttca aggctgagct tttcaacgga      420

aaggtgttga agcagcttgg aactgtgact actactgagc acgagaacgc tttgcttaga      480

tctttcgata agttcactac ttacttctct ggattctacg agaacagaaa gaacgtgttc      540

tctgctgagg atatctctac tgctatccca cacaggatcg tgcaggataa cttcccaaag      600

ttcaaagaga actgccacat cttcactagg cttatcactg ctgtgccatc tcttagggaa      660

cacttcgaga acgtgaagaa ggctatcgga atcttcgtgt ctacttcaat cgaggaagtg      720

ttctctttcc ctttctacaa tcaacttctt actcagactc agattgatct ttacaaccag      780

cttcttggag gaatctcaag agaggctgga actgagaaga tcaagggact taacgaggtt      840

ttgaaccttg ctatccaaaa gaacgatgag actgctcaca ttatcgcttc acttccacac      900

agattcatcc ctttgttcaa gcagatcctt tctgatagga acactttgtc tttcatcctt      960

gaagagttca agtctgatga agaggtgatc cagtctttct gcaagtacaa gactcttctt     1020

aggaacgaga atgtgttgga gactgctgag gctcttttca atgagcttaa ctctatcgat     1080

cttactcaca ttttcatctc tcacaagaag cttgagacta tctcttctgc tctttgcgat     1140

cactgggata ctttgaggaa cgcactttac gagagaagga tctctgagct tactggaaag     1200

atcactaagt ctgctaaaga gaaggttcag agatcactta agcacgagga tatcaacctt     1260

caagagatca tctctgctgc tggaaaagag ctttctgagg ctttcaagca aaagacttct     1320

gagatcttgt ctcacgctca cgctgctctt gatcagccac ttccaactac tcttaagaag     1380

caagaagaga aagagatctt gaagtctcag ttggattctc ttttgggact ttaccacctt     1440

cttgattggt tcgctgtgga tgagtctaac gaagtggatc cagagttctc agctaggttg     1500

actggaatca agttggagat ggaaccatct ctttcattct acaacaaggc tagaaactac     1560

gctactaaga agccatactc tgttgagaag ttcaagctta atttccagat gccaactttg     1620

gcttctggat gggatgtgaa caaagaaaaa aacaacggtg ctatcctttt cgtgaagaac     1680

ggactttact acttgggaat catgccaaag cagaagggaa ggtacaaggc tttgtcattc     1740

gagccaactg aaaagacatc agagggattc gataagatgt actatgatta cttcccagat     1800

gctgctaaga tgatcccaaa gtgctctact cagcttaagg ctgtgacagc tcacttccag     1860

actcacacta ctccaatcct tttgtctaac aacttcatcg agccacttga gatcacaaaa     1920

gaaatctacg atcttaacaa ccctgagaaa gagccaaaaa agttccagac tgcttacgct     1980

aaaaagactg gtgatcagaa gggatacagg gaagctttgt gcaagtggat cgattttact     2040

agggatttct tgtctaagta cactaagact acttctatcg atttgtcatc tttgaggcca     2100

tcttcacagt acaaggatct tggagagtac tacgctgagt tgaacccact tctttaccac     2160

atctcattcc agaggatcgc agagaaagaa atcatggatg ctgttgagac tggaaagctt     2220

taccttttcc aaatctataa caaggatttc gctaagggac accacggaaa gccaaacctt     2280

cacactcttt actggactgg acttttctca ccagagaact tggctaagac ttctatcaag     2340

ttgaacggac aggctgagtt gttctacagg ccaaagtcta ggatgaagag aatggctcac     2400

aggcttggag agaagatgct taacaaaaag ttgaaggatc aaaagactcc tatcccagat     2460

actctttacc aagagcttta cgattacgtg aaccacaggc tttctcacga tctttctgat     2520

gaggctaggg ctcttttgcc aaacgttatc acaaaagagg tgtcacacga gatcatcaag     2580

gatagaaggt ttacttctga taagttcttc ttccacgtgc caatcactct taactaccag     2640

gctgctaact ctccatctaa gttcaaccag agggtgaacg cttaccttaa agagcaccca     2700

gagacaccta tcatcggtat cgatagggga gagaggaacc ttatctacat cactgtgatc     2760

gattctactg gtaagattct tgagcagaga tctttgaaca ctatccagca gttcgattac     2820

cagaagaagt tggataacag ggaaaaagag agggttgcag ctaggcaggc ttggtctgtt     2880

gtgggaacta tcaaggattt gaagcaggga tacttgtctc aggttatcca cgagattgtg     2940

gatttgatga tccactacca agctgtggtg gtgcttgaga accttaactt cggattcaag     3000

tctaagagga ctggtatcgc tgagaaggct gtgtaccaac agttcgagaa gatgttgatc     3060

gataagctta actgccttgt gcttaaggat taccctgctg aaaaggtggg aggtgtgctt     3120

aacccatacc agcttacaga tcagttcact tcattcgcta agatgggaac tcagtctggt     3180

ttcttgttct acgttccagc tccatacaca tcaaagatcg atccattgac tggattcgtg     3240

gatcctttcg tgtggaaaac tattaagaac cacgagtcta ggaagcactt ccttgagggt     3300

ttcgatttcc ttcactacga tgtgaaaact ggtgatttca tcttgcactt taagatgaat     3360

aggaacttgt ctttccagag gggtttgcca ggattcatgc cagcttggga tatcgtgttt     3420

gagaagaacg agacacagtt cgatgctaag ggaactccat tcattgctgg taagaggatt     3480

gtgccagtga ttgagaacca taggttcact ggtaggtaca gggatcttta cccagctaac     3540

gagttgatcg ctttgttgga agagaaggga atcgtgttca gggatggatc taatatcctt     3600

ccaaagcttt tggagaatga tgattctcac gcaatcgata caatggtggc tcttatcaga     3660

tctgtgcttc agatgaggaa ctctaacgct gctactggtg aggattacat caactctcca     3720

gtgagggatc ttaacggtgt gtgcttcgat tctaggttcc agaatcctga gtggccaatg     3780

gatgcagatg ctaacggtgc ttaccacatt gctcttaagg gacagcttct tcttaaccac     3840

ttgaaagagt ctaaggatct taagcttcag aacggaatct ctaaccagga ttggcttgct     3900

tacattcaag agcttaggaa tggaagggga tctcatcacc accaccatca caagcttcca     3960

aaaaagaaga ggaaggttta g                                               3981


<210>  7
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10731 (crRNA 1)

<400>  7
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg tcaaaagacc tttttaattt ctactcttgt agatacttct      120

gaggtttgtg gatctttttt tt                                               142


<210>  8
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10732 (crRNA 2)

<400>  8
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg tcaaaagacc tttttaattt ctactcttgt agatagttcc      120

caaagaagac gacctttttt tt                                               142


<210>  9
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10733 (crRNA 3)

<400>  9
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg tcaaaagacc tttttaattt ctactcttgt agatatcgtc      120

aggtaataca tcggattttt tt                                               142


<210>  10
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10734 (crRNA 4)

<400>  10
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg tcaaaagacc tttttaattt ctactcttgt agatgccata      120

cttgtcgcgg aatacttttt tt                                               142


<210>  11
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10735 (crRNA 5)

<400>  11
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg tcaaaagacc tttttaattt ctactcttgt agattggacc      120

aagaatgctg caaatttttt tt                                               142


<210>  12
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10736 (crRNA 6)

<400>  12
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg tcaaaagacc tttttaattt ctactcttgt agatatttgc      120

agcattcttg gtccattttt tt                                               142


<210>  13
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10737 (crRNA 7)

<400>  13
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattg tcaaaagacc tttttaattt ctactcttgt agattccatt      120

ccagtggagt tgatattttt tt                                               142


<210>  14
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG9943 (crRNA 1.1)

<400>  14
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattt aatttctact cttgtagata cttctgaggt ttgtggatct      120

ttttttt                                                                127


<210>  15
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG9942 (crRNA 2.1)

<400>  15
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattt aatttctact cttgtagata gttcccaaag aagacgacct      120

ttttttt                                                                127


<210>  16
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10533 (crRNA 3.1)

<400>  16
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattt aatttctact cttgtagata tcgtcaggta atacatcgga      120

ttttttt                                                                127


<210>  17
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10106 (crRNA 4.1)

<400>  17
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattt aatttctact cttgtagatg ccatacttgt cgcggaatac      120

ttttttt                                                                127


<210>  18
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10101 (crRNA 5.1)

<400>  18
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattt aatttctact cttgtagatt ggaccaagaa tgctgcaaat      120

ttttttt                                                                127


<210>  19
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10102 (crRNA 6.1)

<400>  19
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattt aatttctact cttgtagata tttgcagcat tcttggtcca      120

ttttttt                                                                127


<210>  20
<211>  127
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  KG10104 (crRNA 7.1)

<400>  20
ggagtgatca aaagtcccac atcgatcagg tgatatatag cagcttagtt tatataatga       60

tagagtcgac atagcgattt aatttctact cttgtagatt ccattccagt ggagttgata      120

ttttttt                                                                127


<210>  21
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scaffold

<400>  21
ccgtctaaaa ctcattcaga atttctacta gtgtagat                               38


<210>  22
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scaffold

<400>  22
ctctacaact gataaagaat ttctactttt gtagat                                 36


<210>  23
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scaffold

<400>  23
gtctggcccc aaattttaat ttctactgtt gtagat                                 36


<210>  24
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scaffold

<400>  24
gtcaaaagac ctttttaatt tctactcttg tagat                                  35


<210>  25
<211>  684
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rAPOBEC1 encoding sequence

<400>  25
atgagcagcg aaaccggtcc ggttgcagtt gatccgaccc tgcgtcgtcg tattgaaccg       60

catgaatttg aagttttttt tgatccgcgt gaactgcgta aagaaacctg tctgctgtat      120

gaaattaatt ggggtggtcg tcatagcatt tggcgtcata ccagccagaa taccaataaa      180

catgttgaag ttaattttat tgaaaaattt accaccgaac gttatttttg tccgaatacc      240

cgttgtagca ttacctggtt tctgagctgg agcccgtgtg gtgaatgtag ccgtgcaatt      300

accgaatttc tgagccgtta tccgcatgtt accctgttta tttatattgc acgtctgtat      360

catcatgcag atccgcgtaa tcgtcagggt ctgcgtgatc tgattagcag cggtgttacc      420

attcagatta tgaccgaaca ggaaagcggt tattgttggc gtaattttgt taattatagc      480

ccgagcaatg aagcacattg gccgcgttat ccgcatctgt gggttcgtct gtatgttctg      540

gaactgtatt gtattattct gggtctgccg ccgtgtctga atattctgcg tcgtaaacag      600

ccgcagctga ccttttttac cattgcactg cagagctgtc attatcagcg tctgccgccg      660

catattctgt gggcaaccgg tctg                                             684


<210>  26
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rAPOBEC1

<400>  26

Met Ser Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg 
1               5                   10                  15      


Arg Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu 
            20                  25                  30          


Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile Asn Trp Gly Gly Arg His 
        35                  40                  45              


Ser Ile Trp Arg His Thr Ser Gln Asn Thr Asn Lys His Val Glu Val 
    50                  55                  60                  


Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg Tyr Phe Cys Pro Asn Thr 
65                  70                  75                  80  


Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys 
                85                  90                  95      


Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg Tyr Pro His Val Thr Leu 
            100                 105                 110         


Phe Ile Tyr Ile Ala Arg Leu Tyr His His Ala Asp Pro Arg Asn Arg 
        115                 120                 125             


Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly Val Thr Ile Gln Ile Met 
    130                 135                 140                 


Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg Asn Phe Val Asn Tyr Ser 
145                 150                 155                 160 


Pro Ser Asn Glu Ala His Trp Pro Arg Tyr Pro His Leu Trp Val Arg 
                165                 170                 175     


Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile Leu Gly Leu Pro Pro Cys 
            180                 185                 190         


Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile 
        195                 200                 205             


Ala Leu Gln Ser Cys His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp 
    210                 215                 220                 


Ala Thr Gly Leu 
225             


<210>  27
<211>  501
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TadA encoding sequence

<400>  27
atgtccgagg tcgagttctc tcatgagtac tggatgaggc acgctctcac tcttgctaaa       60

agagcttggg acgagagaga ggttccagtt ggagctgttt tggtgcacaa caaccgtgtg      120

attggcgaag gatggaacag gccaattgga aggcatgatc caactgctca cgctgagatt      180

atggccctta gacaaggtgg actcgtgatg cagaactaca ggcttatcga cgccactctc      240

tacgtgacac ttgagccatg tgttatgtgc gctggtgcca tgattcactc caggattgga      300

agggttgtgt tcggagctag agatgctaaa actggcgctg ccggatctct catggatgtg      360

cttcatcatc ctgggatgaa ccacagggtt gagatcactg agggaatcct tgctgatgag      420

tgcgctgctc tcctgtctga ttttttcagg atgaggcgtc aagagatcaa ggcccagaag      480

aaggctcagt cctctactga t                                                501


<210>  28
<211>  167
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TadA

<400>  28

Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu 
1               5                   10                  15      


Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala 
            20                  25                  30          


Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro 
        35                  40                  45              


Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg 
    50                  55                  60                  


Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu 
65                  70                  75                  80  


Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His 
                85                  90                  95      


Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly 
            100                 105                 110         


Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His 
        115                 120                 125             


Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu 
    130                 135                 140                 


Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys 
145                 150                 155                 160 


Lys Ala Gln Ser Ser Thr Asp 
                165         


<210>  29
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  29

Glu Ala Ala Ala Lys 
1               5   


<210>  30
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  30

Ser Pro Lys Lys Lys Arg Lys Val Glu Ala Ser 
1               5                   10      


<210>  31
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  31

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser 
1               5                   10                  15      


<210>  32
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  32

Lys Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu 
1               5                   10                  15      


Ser 
    


<210>  33
<211>  252
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UGI encoding sequence

<400>  33
atgactaatc tgtcagatat tattgaaaag gagaccggta agcaactggt tatccaggaa       60

tccatcctca tgctcccaga ggaggtggaa gaagtcattg ggaacaagcc ggaaagcgat      120

atactcgtgc acaccgccta cgacgagagc accgacgaga atgtcatgct tctgactagc      180

gacgcccctg aatacaagcc ttgggctctg gtcatacagg atagcaacgg tgagaacaag      240

attaagatgc tc                                                          252


<210>  34
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UGI

<400>  34

Met Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu 
1               5                   10                  15      


Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val 
            20                  25                  30          


Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp 
        35                  40                  45              


Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu 
    50                  55                  60                  


Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys 
65                  70                  75                  80  


Ile Lys Met Leu 
                


<210>  35
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UGI encoding sequence

<400>  35
accaatctga gcgatatcat tgaaaaagaa accggcaaac agctggtgat tcaagaaagc       60

attctgatgc tgcctgaaga agtggaagaa gttattggta ataaaccgga aagcgatatt      120

ctggttcata ccgcatatga tgaaagcacc gatgaaaatg ttatgctg                   168


<210>  36
<211>  56
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UGI

<400>  36

Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu Val 
1               5                   10                  15      


Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val Ile 
            20                  25                  30          


Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp Glu 
        35                  40                  45              


Ser Thr Asp Glu Asn Val Met Leu 
    50                  55      


<210>  37
<211>  681
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rAPOBEC1 encoding sequence

<400>  37
atgagcagcg aaacaggtcc ggttgcagtt gatccgaccc tgcgtcgtcg tattgaaccg       60

catgaatttg aagttttttt tgatccgcgt gagctgcgta aagaaacctg tctgctgtat      120

gaaattaact ggggtggtcg tcatagcatt tggcgtcata ccagccagaa taccaataaa      180

catgtggaag tgaacttcat cgagaaattt accaccgaac gttatttttg tccgaatacc      240

cgttgtagca ttacctggtt tctgagctgg tcaccgtgtg gtgaatgtag ccgtgcaatt      300

accgaatttc tgagccgtta tccgcatgtt accctgttta tctatattgc ccgtctgtat      360

catcatgcag atccgcgtaa tcgtcagggt ctgcgtgatc tgattagcag cggtgttacc      420

attcagatta tgaccgaaca agaaagcggt tattgctggc gtaattttgt gaattatagc      480

ccgagcaatg aagcacattg gcctcgctat ccgcatctgt gggttcgtct gtatgttctg      540

gaactgtatt gtattattct gggtctgcct ccgtgtctga atattctgcg tcgtaaacag      600

ccgcagctga ccttttttac cattgcactg cagagctgtc attatcagcg tctgccaccg      660

catattctgt gggcaacagg t                                                681


<210>  38
<211>  31
<212>  DNA
<213>  Lycopersicon esculentum

<400>  38
cgtttaactt ctgaggtttg tggatctttt a                                      31


<210>  39
<211>  35
<212>  DNA
<213>  Lycopersicon esculentum

<400>  39
actttcagtt cccaaagaag acgacctcga gctcc                                  35


<210>  40
<211>  31
<212>  DNA
<213>  Lycopersicon esculentum

<400>  40
aatttcatcg tcaggtaata catcggattc a                                      31


<210>  41
<211>  31
<212>  DNA
<213>  Lycopersicon esculentum

<400>  41
ggtttagcca tacttgtcgc ggaatacctt g                                      31


<210>  42
<211>  30
<212>  DNA
<213>  Lycopersicon esculentum

<400>  42
gctttgtgga ccaagaatgc tgcaaatgaa                                        30


<210>  43
<211>  29
<212>  DNA
<213>  Lycopersicon esculentum

<400>  43
tgtttcattt gcagcattct tggtccaca                                         29


<210>  44
<211>  31
<212>  DNA
<213>  Lycopersicon esculentum

<400>  44
tatttctcca ttccagtgga gttgatatac t                                      31


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  45
cgtttaactt ctgaggtttt a                                                 21


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  46
cgtttaactt ctgaggtttg ta                                                22


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  47
cgtttaactt ctgaggtttt a                                                 21


<210>  48
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  48
cgtttaactt ctgaggtttt ta                                                22


<210>  49
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  49
actttcagtt cccaaagaag acgagctcc                                         29


<210>  50
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  50
actttcagtt cccaaagaag acctcgagct cc                                     32


<210>  51
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  51
actttcagtt cccaaagaag acgagctcc                                         29


<210>  52
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  52
actttcagtt cccaaagaag agctcc                                            26


<210>  53
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  53
aatttcatcg tcaggtaata tca                                               23


<210>  54
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  54
aatttcatcg tcaggtaata cattca                                            26


<210>  55
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  55
aatttcatcg tcaggtaata cca                                               23


<210>  56
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  56
aatttcatcg tcaggtaata gattca                                            26


<210>  57
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  57
ggtttagcca tacttgtcgc ttg                                               23


<210>  58
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  58
ggtttagcca tacttgtcgc tg                                                22


<210>  59
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  59
ggtttagcca tacttgtcgc tg                                                22


<210>  60
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  60
ggtttagcca tacttgtcgc g                                                 21


<210>  61
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  61
gctttgtgga ccaagaatga a                                                 21


<210>  62
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  62
gctttgtgga ccaagaatgc tgaa                                              24


<210>  63
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  63
gctttgtgga ccaagaaa                                                     18


<210>  64
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  64
gctttgtgga ccaagaatgc tgaa                                              24


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  65
tgtttcattt gcagcattca                                                   20


<210>  66
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  66
tgtttcattt gcagcattc                                                    19


<210>  67
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  67
tgtttcattt gcagcattct ta                                                22


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  68
tgtttcattt gcagcattct                                                   20


<210>  69
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  69
tatttctcca ttccagtgga gttact                                            26


<210>  70
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  70
tatttctcca ttccagtgga tact                                              24


<210>  71
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  71
tatttctcca ttccagtgga tact                                              24


<210>  72
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant

<400>  72
tatttctcca ttccagtgga ct                                                22


<210>  73
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  scaffold

<400>  73
gttaagttat atagaataat ttctactgtt gtaga                                  35


<210>  74
<211>  3792
<212>  DNA
<213>  artificial sequence

<220>
<223>  MAD7 coding sequence codon optimized for S. lycopersicum

<400>  74
atgaacaacg ggaccaacaa cttccagaac ttcatcggga tcagctccct ccaaaagacc       60

cttaggaacg ctcttatccc aaccgagact acccagcagt tcatcgtgaa gaacgggatc      120

atcaaagagg acgagcttag gggagagaac aggcagatcc tcaaggatat catggacgac      180

tactacaggg gcttcatctc cgagactctg tcctccatcg atgatatcga ctggacctcc      240

ttgttcgaga agatggaaat ccagctcaag aacggggaca acaaggacac ccttatcaaa      300

gagcagaccg agtaccgtaa ggccatccac aaaaagttcg ctaacgacga caggttcaag      360

aatatgttct ccgccaagct catcagcgac atccttccag agttcgtgat ccacaacaac      420

aactactccg cctccgagaa agaggaaaag acccaggtca tcaagctgtt ctccaggttc      480

gctacctcct tcaaggacta cttcaagaac agggccaact gcttctccgc cgacgatatc      540

tcttcatctt cttgccacag gatcgtgaac gacaacgccg agatcttctt ctccaacgct      600

cttgtgtaca ggcgtatcgt gaagtccctc tccaacgatg acatcaacaa gatctccggg      660

gacatgaagg acagcctcaa agagatgtcc ctcgaagaga tctactccta cgagaagtac      720

ggggagttca tcacccaaga gggcatcagc ttctacaacg acatctgcgg aaaggtgaac      780

tccttcatga acctctactg ccagaagaac aaagagaaca agaacctgta caagctccag      840

aagctccaca agcagatcct gtgcattgct gacactagct acgaggtgcc atacaagttc      900

gagtccgatg aagaggtgta ccagtccgtg aatggcttcc tcgacaacat ctcctctaag      960

cacatcgtcg agaggcttag gaagatcggc gataactaca acgggtacaa cctggacaag     1020

atctacatcg tgtccaagtt ctacgagtcc gtgagccaga aaacctaccg tgattgggag     1080

actatcaaca ccgctctcga gattcactac aacaacattc tccccggcaa cgggaaatcc     1140

aaggctgata aggttaagaa ggccgtcaag aacgacctcc agaagtctat cactgagatc     1200

aacgagctgg tgtccaacta caagctctgc tccgatgaca acatcaaggc cgagacttac     1260

atccacgaga tctcccacat cctgaacaac ttcgaggccc aagagctgaa gtacaaccca     1320

gagattcacc tcgtcgagtc tgagcttaag gcctccgagc ttaagaacgt gctcgacgtt     1380

atcatgaacg ccttccattg gtgctccgtg ttcatgactg aagagttggt ggacaaggat     1440

aacaacttct acgccgagct ggaagaaatc tacgacgaga tctaccccgt gatctccctc     1500

tacaacctcg tgaggaatta cgtgacccag aagccatact ccaccaagaa gatcaagctc     1560

aacttcggga tcccaacctt ggctgatgga tggtccaagt ccaaagagta ctccaacaac     1620

gccatcatcc tgatgaggga caacctgtac tacctcggga tcttcaacgc caagaacaag     1680

cccgacaaga agattatcga ggggaacacc tctgagaaca agggcgacta caaaaagatg     1740

atctacaact tgctcccagg gcctaacaag atgatcccca aggtgttcct cagctccaag     1800

actggtgttg agacatacaa gccctccgcc tacattctcg agggatacaa gcaaaacaag     1860

cacatcaagt cctccaagga cttcgatatc accttctgcc acgacctcat cgattatttc     1920

aagaactgca ttgccattca tccagagtgg aagaacttcg ggttcgactt ctccgatacc     1980

tccacctacg aggatatctc cggattctac cgtgaggttg agcttcaggg gtacaagatc     2040

gattggacct acatcagcga gaaggacatc gacctgttgc aagagaaggg gcagctttac     2100

ctcttccaaa tctacaacaa agacttctcc aaaaagtcca ccggcaacga caacctccac     2160

accatgtacc tcaagaacct cttcagcgaa gagaacctca aggacattgt gctcaagctg     2220

aatggcgagg ctgagatttt ctttaggaag tcctctatca agaaccccat catccacaag     2280

aagggctcca tcctcgttaa caggacttac gaggctgaag agaaggacca gttcgggaac     2340

attcagatcg tgcgtaagaa catccccgag aacatctacc aagagcttta caagtacttc     2400

aacgacaagt ccgacaaaga gctgtctgac gaggctgcca agttgaagaa tgtggtggga     2460

catcatgagg ccgctaccaa cattgtgaag gactacaggt acacttacga caagtatttc     2520

ttgcacatgc ccatcaccat caacttcaag gccaacaaga ccggcttcat caacgacagg     2580

atcctccagt acattgccaa agaaaaggac ctccacgtga tcgggattga taggggtgag     2640

cgaaacctca tctacgtgtc agtgatcgat acctgcggga acatcgttga gcagaagtcc     2700

ttcaacatcg ttaacgggta cgactaccag attaagctca agcagcaaga gggtgctaga     2760

cagatcgcta ggaaagagtg gaaagagatc gggaaaatca aagagatcaa agaaggctac     2820

ctctccttgg tgattcacga gatcagcaag atggtgatca agtacaacgc tattatcgcc     2880

atggaagatc tcagctacgg cttcaagaag gggcgtttta aggttgagag gcaggtctac     2940

caaaagttcg agactatgct gatcaacaag ctgaactacc tggtgtttaa ggacatctcc     3000

attaccgaga acggcgggct tcttaaggga taccagctca cttacattcc cgacaaactg     3060

aagaacgtgg gtcaccaatg cggctgcatt ttctatgttc cagctgccta cacctccaag     3120

atcgacccaa ctactggatt cgtgaacatc ttcaagttca aggacctcac cgtggacgct     3180

aagagggaat tcatcaagaa gttcgactcc atcaggtacg actccgagaa gaaccttttc     3240

tgcttcacct tcgattacaa caactttatc acccagaaca ccgtgatgtc caagagcagc     3300

tggtcagtgt acacatacgg tgtgaggatc aagcgacgtt tcgtgaacgg aaggttcagc     3360

aacgagagcg atacaatcga catcactaag gacatggaaa agactcttga gatgaccgac     3420

atcaactggc gtgatggtca tgatctcagg caggacatta tcgactacga gatcgtgcag     3480

cacatcttcg aaatcttcag gctcactgtg cagatgagga actcccttag tgagcttgag     3540

gatagggact acgacaggct tatctcccca gtgcttaacg agaacaacat cttctacgac     3600

agcgctaagg ctggggatgc tttgccaaaa gatgctgatg ctaacggggc ttactgtatc     3660

gctttgaagg ggctgtacga gattaagcag atcaccgaga actggaaaga ggatggcaag     3720

ttctcccgtg acaagctcaa gatctctaac aaggactggt tcgatttcat tcagaacaag     3780

cgttacctct ga                                                         3792


