                         SEQUENCE LISTING

<110>  Leidos, Inc.
 
<120>  MATERIALS AND METHODS FOR CELL-FREE EXPRESSION OF VACCINE EPITOPE
        CONCATEMERS

<130>  LEID0010-PRO4

<150>  US 62/693,904
<151>  2018-07-03

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  181
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  1

Ser Val Phe Leu Gly Thr Met Ile Val Val Gly Met Gly Gln Asp Lys 
1               5                   10                  15      


Glu Ala Ala Ala Ser Glu Gln Lys Thr Thr Leu Lys Arg Gly Asp Tyr 
            20                  25                  30          


Phe Asp Phe Thr Leu Ser Asn Asn Val Asn Thr Tyr Gly Val Ser Thr 
        35                  40                  45              


Ala Arg Ala Ile Asp Gly Glu Gly Gly Tyr Val Asp Gly Tyr Ile Glu 
    50                  55                  60                  


Thr Ile Glu Glu Thr Asp Ser Ser Ala Ile Asp Ile His Ser Gly Leu 
65                  70                  75                  80  


Gly Thr Glu Asn Gly His Gly Asn Tyr Gly Val Ile Glu Glu Ile Glu 
                85                  90                  95      


Glu Asn Ser His Val Asp Ile Lys Lys Tyr Glu Gln Gly Gly Asn Ile 
            100                 105                 110         


Val Asp Ile Asp Phe Asp Ser Val Pro Gln Ile Gln Gly Gln Asn Asn 
        115                 120                 125             


Gly Asn Lys Tyr Glu Gln Gly Gly Asn Ile Ile Asp Ile Asp Phe Asp 
    130                 135                 140                 


Ser Val Pro Gln Ile His Gly Phe Asn Lys His Thr Gly Gly His Asn 
145                 150                 155                 160 


Ser Val Asp Phe Glu Glu Asp Thr Leu Pro Lys Val Ser Gly Gln Asn 
                165                 170                 175     


Glu Gly Gln Gln Thr 
            180     


<210>  2
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbA.1

<400>  2

Ser Val Phe Leu Gly Thr Met Ile Val Val Gly Met Gly Gln Asp Lys 
1               5                   10                  15      


Glu Ala Ala Ala Ser Glu Gln Lys Thr Thr 
            20                  25      


<210>  3
<211>  24
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbA.2

<400>  3

Leu Lys Arg Gly Asp Tyr Phe Asp Phe Thr Leu Ser Asn Asn Val Asn 
1               5                   10                  15      


Thr Tyr Gly Val Ser Thr Ala Arg 
            20                  


<210>  4
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbA.3

<400>  4

Ala Ile Asp Gly Glu Gly Gly Tyr Val Asp Gly Tyr Ile Glu Thr Ile 
1               5                   10                  15      


Glu Glu Thr Asp Ser Ser Ala Ile Asp Ile 
            20                  25      


<210>  5
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbA.4

<400>  5

Gly Leu Gly Thr Glu Asn Gly His Gly Asn Tyr Gly Val Ile Glu Glu 
1               5                   10                  15      


Ile Glu Glu Asn Ser His Val Asp Ile Lys 
            20                  25      


<210>  6
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbA.5

<400>  6

Lys Tyr Glu Gln Gly Gly Asn Ile Val Asp Ile Asp Phe Asp Ser Val 
1               5                   10                  15      


Pro Gln Ile Gln Gly Gln Asn Asn Gly Asn 
            20                  25      


<210>  7
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbA.6

<400>  7

Lys Tyr Glu Gln Gly Gly Asn Ile Ile Asp Ile Asp Phe Asp Ser Val 
1               5                   10                  15      


Pro Gln Ile His Gly Phe Asn Lys His Thr 
            20                  25      


<210>  8
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbA.7

<400>  8

Gly Gly His Asn Ser Val Asp Phe Glu Glu Asp Thr Leu Pro Lys Val 
1               5                   10                  15      


Ser Gly Gln Asn Glu Gly Gln Gln Thr Ile 
            20                  25      


<210>  9
<211>  206
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  9

Ser Val Phe Leu Gly Thr Met Ile Val Val Gly Met Gly Gln Glu Lys 
1               5                   10                  15      


Glu Ala Ala Ala Ser Glu Gln Asn Asn Thr Ile Lys Pro Gly Asp Tyr 
            20                  25                  30          


Phe Asp Phe Thr Leu Ser Asn Asn Val Glu Thr His Gly Ile Ser Pro 
        35                  40                  45              


Leu Arg Ser Glu Pro Ile Glu Leu Asp Ile Lys Ser Glu Pro Pro Val 
    50                  55                  60                  


Glu Lys His Glu Leu Thr Gly Thr Ile Glu Glu Ser Asn Asp Ser Lys 
65                  70                  75                  80  


Pro Ile Asp Phe Glu Tyr His Thr Ala Val Glu Gly Val Glu Gly His 
                85                  90                  95      


Ala Glu Gly Leu Gly Thr Glu Asn Gly His Gly Asn Tyr Gly Val Ile 
            100                 105                 110         


Glu Glu Ile Glu Glu Asn Ser His Val Asp Ile Lys Glu Glu Asp Lys 
        115                 120                 125             


Pro Lys Tyr Glu Gln Gly Gly Asn Ile Val Asp Ile Asp Phe Asp Ser 
    130                 135                 140                 


Val Pro Gln Ile His Gly Gln Asn Lys Gly Asp Tyr Glu His Gly Gly 
145                 150                 155                 160 


Asn Ile Ile Asp Ile Asp Phe Asp Ser Val Pro His Ile His Gly Phe 
                165                 170                 175     


Asn Lys His Thr Gly Gly His Asn Ser Val Asp Phe Glu Glu Asp Thr 
            180                 185                 190         


Leu Pro Gln Val Ser Gly His Asn Glu Gly Gln Gln Thr Ile 
        195                 200                 205     


<210>  10
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbB.1

<400>  10

Ser Val Phe Leu Gly Thr Met Ile Val Val Gly Met Gly Gln Glu Lys 
1               5                   10                  15      


Glu Ala Ala Ala Ser Glu Gln Asn Asn Thr 
            20                  25      


<210>  11
<211>  24
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbB.2

<400>  11

Ile Lys Pro Gly Asp Tyr Phe Asp Phe Thr Leu Ser Asn Asn Val Glu 
1               5                   10                  15      


Thr His Gly Ile Ser Pro Leu Arg 
            20                  


<210>  12
<211>  48
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbB.3 and 4

<400>  12

Ser Glu Pro Ile Glu Leu Asp Ile Lys Ser Glu Pro Pro Val Glu Lys 
1               5                   10                  15      


His Glu Leu Thr Gly Thr Ile Glu Glu Ser Asn Asp Ser Lys Pro Ile 
            20                  25                  30          


Asp Phe Glu Tyr His Thr Ala Val Glu Gly Val Glu Gly His Ala Glu 
        35                  40                  45              


<210>  13
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbB.5

<400>  13

Gly Leu Gly Thr Glu Asn Gly His Gly Asn Tyr Gly Val Ile Glu Glu 
1               5                   10                  15      


Ile Glu Glu Asn Ser His Val Asp Ile Lys 
            20                  25      


<210>  14
<211>  31
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbB.6

<400>  14

Glu Glu Asp Lys Pro Lys Tyr Glu Gln Gly Gly Asn Ile Val Asp Ile 
1               5                   10                  15      


Asp Phe Asp Ser Val Pro Gln Ile His Gly Gln Asn Lys Gly Asp 
            20                  25                  30      


<210>  15
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbB.7

<400>  15

Tyr Glu His Gly Gly Asn Ile Ile Asp Ile Asp Phe Asp Ser Val Pro 
1               5                   10                  15      


His Ile His Gly Phe Asn Lys His Thr 
            20                  25  


<210>  16
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FnbB.8

<400>  16

Gly Gly His Asn Ser Val Asp Phe Glu Glu Asp Thr Leu Pro Gln Val 
1               5                   10                  15      


Ser Gly His Asn Glu Gly Gln Gln Thr Ile 
            20                  25      


<210>  17
<211>  182
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  17

Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Asp Thr 
1               5                   10                  15      


Val Tyr Pro His Gln Ala Gly Tyr Val Lys Gly Val Ile Asp Ser Asp 
            20                  25                  30          


Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asp Thr Lys Glu Asn 
        35                  40                  45              


Val Thr Ala Asn Ile Thr Met Pro Ala Tyr Ile Asp Pro Glu Asn Val 
    50                  55                  60                  


Thr Lys Thr Gly Asn Val Thr Leu Thr Thr Gly Ile Gly Ser Asn Phe 
65                  70                  75                  80  


Glu Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln 
                85                  90                  95      


Tyr Lys Val Glu Phe Asn Thr Pro Thr Leu Asp Leu Pro Gln Ser Ser 
            100                 105                 110         


Pro Gln Thr Ile Ser Asn Ala Gln Gly Thr Ser Lys Pro Ser Val Arg 
        115                 120                 125             


Thr Arg Ala Lys Asp Phe Gln Leu Glu Lys Thr Thr Phe Asp Pro Asn 
    130                 135                 140                 


Gln Ser Gly Asn Thr Phe Met Ala Ala Asn Phe Thr Phe Ser Leu Pro 
145                 150                 155                 160 


Leu Phe Thr Asp Arg Ala Lys Ala Pro Lys Ser Gly Thr Tyr Asp Ala 
                165                 170                 175     


Asn Ile Asn Ile Ala Asp 
            180         


<210>  18
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ClfA.1

<400>  18

Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly Asp Thr 
1               5                   10                  15      


Val Tyr Pro His Gln Ala Gly Tyr Val Lys 
            20                  25      


<210>  19
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ClfA.2

<400>  19

Gly Val Ile Asp Ser Asp Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr 
1               5                   10                  15      


Val Asp Thr Lys Glu Asn Val Thr Ala Asn 
            20                  25      


<210>  20
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ClfA.3

<400>  20

Ile Thr Met Pro Ala Tyr Ile Asp Pro Glu Asn Val Thr Lys Thr Gly 
1               5                   10                  15      


Asn Val Thr Leu Thr Thr Gly Ile Gly Ser 
            20                  25      


<210>  21
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ClfA.4

<400>  21

Asn Phe Glu Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro 
1               5                   10                  15      


Asn Gln Tyr Lys Val Glu Phe Asn Thr Pro 
            20                  25      


<210>  22
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ClfB.1

<400>  22

Thr Leu Asp Leu Pro Gln Ser Ser Pro Gln Thr Ile Ser Asn Ala Gln 
1               5                   10                  15      


Gly Thr Ser Lys Pro Ser Val Arg Thr Arg 
            20                  25      


<210>  23
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ClfB.2

<400>  23

Ala Lys Asp Phe Gln Leu Glu Lys Thr Thr Phe Asp Pro Asn Gln Ser 
1               5                   10                  15      


Gly Asn Thr Phe Met Ala Ala Asn Phe Thr 
            20                  25      


<210>  24
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  ClfB.3

<400>  24

Phe Ser Leu Pro Leu Phe Thr Asp Arg Ala Lys Ala Pro Lys Ser Gly 
1               5                   10                  15      


Thr Tyr Asp Ala Asn Ile Asn Ile Ala Asp 
            20                  25      


<210>  25
<211>  225
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  25

Leu Thr Ser Glu Leu Asn Thr Gln Ser Glu Thr Val Gly Asn Gln Asn 
1               5                   10                  15      


Ser Thr Thr Ile Glu Ala Ser Thr Ser Thr Trp Ile Ala Lys Ser Gly 
            20                  25                  30          


Thr Thr Asn Phe Ser Leu Ser Met Thr Ala Ser Thr Gly Gly Ala Thr 
        35                  40                  45              


Asn Leu Gln Gln Val Thr Gly Leu Pro Ser Gly Leu Thr Phe Asp Ser 
    50                  55                  60                  


Thr Asn Asn Thr Ile Ser Gly Thr Pro Thr Asn Ile Gly Thr Val Ser 
65                  70                  75                  80  


Leu Ser Asp Ser Val Ser Ala Ser Lys Ser Leu Ser Thr Ser Glu Ser 
                85                  90                  95      


Asn Ser Val Ser Ser Ser Thr Ser Thr Ser Leu Val Asn Ser Gln Ser 
            100                 105                 110         


Val Ser Ser Ser Met Ser Asp Ser Gly Ser Gln Ser Met Ser Asp Ser 
        115                 120                 125             


Val Ser Thr Ser Asp Ser Ser Ser Leu Ser Val Ser Thr Ser Leu Arg 
    130                 135                 140                 


Ser Ser Ala Ser Glu Ser Asp Ser Met Ser Thr Ser Asp Ser Ser Ser 
145                 150                 155                 160 


Ile Ser Gly Ser Asn Ser Thr Ser Thr Ser Leu Ser Thr Ile Ser Ile 
                165                 170                 175     


Ser Gly Ser Gln Ser Thr Val Glu Ser Glu Ser Thr Ser Asp Ser Thr 
            180                 185                 190         


Ser Ile Ser Asp Ser Glu Ser Ser Glu Ser Val Ser Thr Ser Thr Ser 
        195                 200                 205             


Thr Ser Leu Ser Thr Ser Asp Ser Thr Ser Thr Ser Glu Ser Leu Ser 
    210                 215                 220                 


Thr 
225 


<210>  26
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SraP.1

<400>  26

Leu Thr Ser Glu Leu Asn Thr Gln Ser Glu Thr Val Gly Asn Gln Asn 
1               5                   10                  15      


Ser Thr Thr Ile Glu Ala Ser Thr Ser Thr 
            20                  25      


<210>  27
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SraP.2

<400>  27

Trp Ile Ala Lys Ser Gly Thr Thr Asn Phe Ser Leu Ser Met Thr Ala 
1               5                   10                  15      


Ser Thr Gly Gly Ala Thr Asn Leu Gln Gln 
            20                  25      


<210>  28
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SraP.3

<400>  28

Val Thr Gly Leu Pro Ser Gly Leu Thr Phe Asp Ser Thr Asn Asn Thr 
1               5                   10                  15      


Ile Ser Gly Thr Pro Thr Asn Ile Gly Thr 
            20                  25      


<210>  29
<211>  41
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SraP.4

<400>  29

Val Ser Leu Ser Asp Ser Val Ser Ala Ser Lys Ser Leu Ser Thr Ser 
1               5                   10                  15      


Glu Ser Asn Ser Val Ser Ser Ser Thr Ser Thr Ser Leu Val Asn Ser 
            20                  25                  30          


Gln Ser Val Ser Ser Ser Met Ser Asp 
        35                  40      


<210>  30
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SraP.5

<400>  30

Ser Gly Ser Gln Ser Met Ser Asp Ser Val Ser Thr Ser Asp Ser Ser 
1               5                   10                  15      


Ser Leu Ser Val Ser Thr Ser Leu Arg Ser 
            20                  25      


<210>  31
<211>  28
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SraP.6

<400>  31

Ser Ala Ser Glu Ser Asp Ser Met Ser Thr Ser Asp Ser Ser Ser Ile 
1               5                   10                  15      


Ser Gly Ser Asn Ser Thr Ser Thr Ser Leu Ser Thr 
            20                  25              


<210>  32
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SraP.7

<400>  32

Ile Ser Ile Ser Gly Ser Gln Ser Thr Val Glu Ser Glu Ser Thr Ser 
1               5                   10                  15      


Asp Ser Thr Ser Ile Ser Asp Ser Glu Ser 
            20                  25      


<210>  33
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  SraP.8

<400>  33

Ser Glu Ser Val Ser Thr Ser Thr Ser Thr Ser Leu Ser Thr Ser Asp 
1               5                   10                  15      


Ser Thr Ser Thr Ser Glu Ser Leu Ser Thr 
            20                  25      


<210>  34
<211>  193
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  34

Pro Gln Gly Tyr Gly Ser Tyr Asn Ser Phe Ser Ile Asn Tyr Lys Thr 
1               5                   10                  15      


Lys Ile Thr Asn Glu Gln Gln Lys Glu Phe Asn Lys Asn Ala Phe Ala 
            20                  25                  30          


Met Asp Lys Ser His Pro Glu Pro Ile Glu Asp Asn Asp Lys His Glu 
        35                  40                  45              


Thr Ile Lys Glu Leu Thr Ile Ala Ala Ile Gly Ile Thr Thr Thr Thr 
    50                  55                  60                  


Ile Ala Ser Thr Ala Asp Ala Ser Glu Gly Tyr Gly Pro Arg Glu Lys 
65                  70                  75                  80  


Lys Pro Val Ser Ile Asn His Asn Ile Asp Tyr Asn Ile Leu Glu Phe 
                85                  90                  95      


Asn Asp Gly Thr Phe Glu Tyr Gly Ala Arg Pro Gln Phe Asn Lys Pro 
            100                 105                 110         


Ala Ala Lys Val Pro Ser Gly Ala Ser Thr Gly Glu His Glu Ala Val 
        115                 120                 125             


Glu Leu Arg Asp Gly Asp Lys Ser Arg Tyr Leu Gly Lys Lys Arg Gly 
    130                 135                 140                 


Leu Glu Thr Ala Val Gly Asp Glu Gly Gly Phe Ala Pro Lys Phe Glu 
145                 150                 155                 160 


Gly Thr Glu Asp Ala Val Glu Glu Phe Tyr Glu Asn Gly Val Tyr Asp 
                165                 170                 175     


Tyr Ser Lys Phe Glu Gly Glu His Gly Ala Lys Arg Thr Ala Ala Glu 
            180                 185                 190         


Gln 
    


<210>  35
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Cna.1

<400>  35

Pro Gln Gly Tyr Gly Ser Tyr Asn Ser Phe Ser Ile Asn Tyr Lys Thr 
1               5                   10                  15      


Lys Ile Thr Asn Glu Gln Gln Lys Glu Phe 
            20                  25      


<210>  36
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  EbpS.1

<400>  36

Asn Lys Asn Ala Phe Ala Met Asp Lys Ser His Pro Glu Pro Ile Glu 
1               5                   10                  15      


Asp Asn Asp Lys His Glu Thr Ile Lys Glu 
            20                  25      


<210>  37
<211>  37
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Efb.1

<400>  37

Leu Thr Ile Ala Ala Ile Gly Ile Thr Thr Thr Thr Ile Ala Ser Thr 
1               5                   10                  15      


Ala Asp Ala Ser Glu Gly Tyr Gly Pro Arg Glu Lys Lys Pro Val Ser 
            20                  25                  30          


Ile Asn His Asn Ile 
        35          


<210>  38
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Efb.2

<400>  38

Asp Tyr Asn Ile Leu Glu Phe Asn Asp Gly Thr Phe Glu Tyr Gly Ala 
1               5                   10                  15      


Arg Pro Gln Phe Asn Lys Pro Ala Ala Lys 
            20                  25      


<210>  39
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Eno.1

<400>  39

Val Pro Ser Gly Ala Ser Thr Gly Glu His Glu Ala Val Glu Leu Arg 
1               5                   10                  15      


Asp Gly Asp Lys Ser Arg Tyr Leu Gly Lys 
            20                  25      


<210>  40
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Eno.2

<400>  40

Lys Arg Gly Leu Glu Thr Ala Val Gly Asp Glu Gly Gly Phe Ala Pro 
1               5                   10                  15      


Lys Phe Glu Gly Thr Glu Asp Ala Val Glu 
            20                  25      


<210>  41
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Eno.3

<400>  41

Glu Phe Tyr Glu Asn Gly Val Tyr Asp Tyr Ser Lys Phe Glu Gly Glu 
1               5                   10                  15      


His Gly Ala Lys Arg Thr Ala Ala Glu Gln 
            20                  25      


