                         SEQUENCE LISTING

<110>  Nussbaum, Ofer
       Musafia, Boaz
 
<120>  AptuDrugs for Increasing and controlling aptamers oligopeptides 
       and small molecules pharmacokinetic in circulation

<130>  US 62/763,822

<140>  US 62/763,822
<141>  2018-07-05

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  aSA-Apt / anti-MUC-1,  AptuDrug


<220>
<221>  misc_feature
<222>  (47)..(65)
<223>  n is deoxyribose 5-phosphate

<300>
<301>  Nabavinia MS, Gholoobi A2, Charbgoo F, Nabavinia M, Ramezani M, 
       Abnous K.
<302>  Anti-MUC1 aptamer: A potential opportunity for cancer treatment
<303>  Med Res Rev
<304>  37
<305>  6
<306>  1518-1538
<307>  2017-07-31
<313>  (66)..(135)

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (1)..(47)

<400>  1
agattgcact tactatctcc acccatatga attgaatacc ctggtttnnn nnnnnnnnnn       60

nnnnngagac aagaataaac gctcaagaag tgaaaatgac agaacacaac attcgacagg      120

aggctcacaa caggc                                                       135


<210>  2
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-Listeria /anti-Muc-1,    AptuDrug


<220>
<221>  misc_feature
<222>  (46)..(63)
<223>  n is deoxyribose 5-phosphate

<300>
<301>  Suh SH, Jaykus LA.
<302>  Nucleic acid aptamers for capture and detection of Listeria spp
<303>  J Biotechnol
<304>  167
<305>  4
<306>  454-461
<307>  2013-09-20
<313>  (1)..(45)

<300>
<301>  Nabavinia MS, Gholoobi A2, Charbgoo F, Nabavinia M, Ramezani M, 
       Abnous K.
<302>  Anti-MUC1 aptamer: A potential opportunity for cancer treatment.
<303>  J Biotechnol
<304>  37
<305>  6
<306>  1518-1539
<307>  2017-11-11
<313>  (64)..(133)

<300>
<313>  (1)..(45)

<400>  2
atcgatgatc tggtcgccgt aacactaccc acatatacga ccaggnnnnn nnnnnnnnnn       60

nnngagacaa gaataaacgc tcaagaagtg aaaatgacag aacacaacat tcgacaggag      120

gctcacaaca ggc                                                         133


<210>  3
<211>  136
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cy5-5'- aSA-Apt / anti-MUC-1,  AptuDrug


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is the fluorescent dye Cy5  or in IUPAC name: 
       6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-y
       l)penta-2,4-dienylidene]indol-1-yl]hexanoic acid

<220>
<221>  misc_feature
<222>  (48)..(66)
<223>  n is deoxyribose 5-phosphate

<300>
<301>  Nabavinia MS, Gholoobi A2, Charbgoo F, Nabavinia M, Ramezani M, 
       Abnous K.
<302>  Anti-MUC1 aptamer: A potential opportunity for cancer treatment
<303>  Med Res Rev
<304>  37
<305>  6
<306>  1518-1538
<307>  2017-07-31
<313>  (67)..(136)

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (2)..(47)

<400>  3
nagattgcac ttactatctc cacccatatg aattgaatac cctggtttnn nnnnnnnnnn       60

nnnnnngaga caagaataaa cgctcaagaa gtgaaaatga cagaacacaa cattcgacag      120

gaggctcaca acaggc                                                      136


<210>  4
<211>  134
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cy5-5'- anti-Listeria /anti-Muc-1,  AptuDrug


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is the fluorescent dye Cy5  or in IUPAC name: 
       6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-y
       l)penta-2,4-dienylidene]indol-1-yl]hexanoic acid

<220>
<221>  misc_feature
<222>  (47)..(64)
<223>  n is deoxyribose 5-phosphate

<300>
<301>  Nabavinia MS, Gholoobi A2, Charbgoo F, Nabavinia M, Ramezani M, 
       Abnous K.
<302>  Anti-MUC1 aptamer: A potential opportunity for cancer treatment
<303>  Med Res Rev
<304>  37
<305>  6
<306>  1518-1538
<307>  2017-07-31
<313>  (65)..(134)

<300>
<301>  Suh SH, Jaykus LA.
<302>  Nucleic acid aptamers for capture and detection of Listeria spp
<303>  Med Res Rev
<304>  167
<305>  4
<306>  454-461
<307>  2013-09-20
<313>  (1)..(45)

<300>
<301>  Suh SH, Jaykus LA.
<302>  Nucleic acid aptamers for capture and detection of Listeria spp
<303>  Med Res Rev
<304>  167
<305>  4
<306>  454-461
<307>  2013-09-20
<313>  (2)..(46)

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (1)..(47)

<400>  4
natcgatgat ctggtcgccg taacactacc cacatatacg accaggnnnn nnnnnnnnnn       60

nnnngagaca agaataaacg ctcaagaagt gaaaatgaca gaacacaaca ttcgacagga      120

ggctcacaac aggc                                                        134


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-NH2 labeled anti-sense to anti-MUC-1 aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is NH2, amine group

<220>
<221>  misc_feature
<222>  (47)..(65)
<223>  n is deoxyribose 5-phosphate

<300>
<301>  Nabavinia MS, Gholoobi A2, Charbgoo F, Nabavinia M, Ramezani M, 
       Abnous K.
<302>  Anti-MUC1 aptamer: A potential opportunity for cancer treatment
<303>  Med Res Rev
<304>  37
<305>  6
<306>  1518-1538
<307>  2017-07-31
<313>  (2)..(24)

<400>  5
naatgttgtg ttctgtcatt ttca                                              24


<210>  6
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-biotin labeled ani-sense to anti-Serum albumin aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is biotin

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (2)..(25)

<400>  6
nggtattcaa tatggggata ctaag                                             25


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-biotin labeled anti-sense to anti- Listeria aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is biotin

<300>
<301>  Suh SH, Jaykus LA.
<302>  Nucleic acid aptamers for capture and detection of Listeria spp
<303>  Med Res Rev
<304>  167
<305>  4
<306>  454-461
<307>  2013-09-20
<313>  (2)..(24)

<400>  7
ngtatatgat gggtagttac ggcg                                              24


<210>  8
<211>  173
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-IgG / anti-MUC-1,  AptuDrug


<220>
<221>  misc_feature
<222>  (86)..(94)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (95)..(103)
<223>  n is deoxyribose 5-phosphate

<300>
<301>  Nabavinia MS, Gholoobi A2, Charbgoo F, Nabavinia M, Ramezani M, 
       Abnous K.
<302>  Anti-MUC1 aptamer: A potential opportunity for cancer treatment
<303>  Med Res Rev
<304>  37
<305>  6
<306>  1518-1538
<307>  2017-07-31
<313>  (104)..(173)

<300>
<301>  Ma J, Wang MG, Mao AH, Zeng JY, Liu YQ, Wang XQ, Ma J, Tian YJ, 
       Ma N, Yang N, Wang L, Liao SQ.
<302>  Target replacement strategy for selection of DNA aptamers against
        the Fc region of mouse IgG
<303>  Genet Mol Res
<304>  12
<305>  2
<306>  1399-1410
<307>  2013-04-25
<313>  (1)..(94)

<400>  8
taatacgact cactatagca atggtacggt acttccccac tcaccgggta cctgccgctc       60

ccaaaagtgc acgctacttt gctaannnnn nnnnnnnnnn nnngagacaa gaataaacgc      120

tcaagaagtg aaaatgacag aacacaacat tcgacaggag gctcacaaca ggc             173


<210>  9
<211>  173
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cy5 -5'- labeled Anti-IgG / anti-MUC-1 AptuDrug


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is the fluorescent dye Cy5  or in IUPAC name: 
       6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-y
       l)penta-2,4-dienylidene]indol-1-yl]hexanoic acid

<220>
<221>  misc_feature
<222>  (86)..(95)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (96)..(104)
<223>  n is deoxyribose 5-phosphate

<300>
<301>  Nabavinia MS, Gholoobi A2, Charbgoo F, Nabavinia M, Ramezani M, 
       Abnous K.
<302>  Anti-MUC1 aptamer: A potential opportunity for cancer treatment
<303>  Med Res Rev
<304>  37
<305>  6
<306>  1518-1538
<307>  2017-07-31
<313>  (105)..(173)

<300>
<301>  Ma J, Wang MG, Mao AH, Zeng JY, Liu YQ, Wang XQ, Ma J, Tian YJ, 
       Ma N, Yang N, Wang L, Liao SQ.
<302>  Target replacement strategy for selection of DNA aptamers against
        the Fc region of mouse IgG
<303>  Genet Mol Res
<304>  12
<305>  2
<306>  1399-1410
<307>  2013-04-25
<313>  (2)..(95)

<400>  9
taatacgact cactatagca atggtacggt acttccccac tcaccgggta cctgccgctc       60

ccaaaagtgc acgctacttt gctaannnnn nnnnnnnnnn nnngagacaa gaataaacgc      120

tcaagaagtg aaaatgacag aacacaacat tcgacaggag gctcacaaca ggc             173


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5'-biotin labeled anti-sense to anti mouse -IgG aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is biotin

<300>
<301>  Ma J, Wang MG, Mao AH, Zeng JY, Liu YQ, Wang XQ, Ma J, Tian YJ, 
       Ma N, Yang N, Wang L, Liao SQ.
<302>  Target replacement strategy for selection of DNA aptamers against
        the Fc region of mouse IgG
<303>  Genet Mol Res
<304>  12
<305>  2
<306>  1399-1410
<307>  2013-04-25
<313>  (2)..(24)

<400>  10
nagtggggaa gtaccgtacc attg                                              24


<210>  11
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-SA aptamer / 6-His Tag,  AptuDrug


<220>
<221>  misc_feature
<222>  (48)..(48)
<223>  n in 6-His tag attached to 3'

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (1)..(47)

<400>  11
agattgcact tactatctcc acccatatga attgaatacc ctggtttn                    48


<210>  12
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cy5-5' labeled Anti-SA aptamer / 6-His Tag,  AptuDrug


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is the fluorescent dye Cy5  or in IUPAC name: 
       6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-y
       l)penta-2,4-dienylidene]indol-1-yl]hexanoic acid

<220>
<221>  misc_feature
<222>  (49)..(49)
<223>  n in 6-His tag attached to 3'

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (2)..(48)

<400>  12
nagattgcac ttactatctc cacccatatg aattgaatac cctggtttn                   49


<210>  13
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-Listeria aptamer / 6-His Tag , AptuDrug


<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  n in 6-His tag attached to 3'

<300>
<301>  Suh SH, Jaykus LA.
<302>  Nucleic acid aptamers for capture and detection of Listeria spp
<303>  Med Res Rev
<304>  167
<305>  4
<306>  454-461
<307>  2013-09-20
<313>  (46)..(46)

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (1)..(45)

<400>  13
atcgatgatc tggtcgccgt aacactaccc acatatacga ccaggn                      46


<210>  14
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-NH2 labeled anti-sense to serum albumin aptamer


<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n in 6-His tag attached to 3'

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (1)..(24)

<400>  14
ggtattcaat atggggatac taagn                                             25


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'-NH2 labeled anti-sense to Listeria aptamer


<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is NH2 amine group attached to 3'

<300>
<301>  Suh SH, Jaykus LA.
<302>  Nucleic acid aptamers for capture and detection of Listeria spp
<303>  Med Res Rev
<304>  167
<305>  4
<306>  454-461
<307>  2013-09-20
<313>  (1)..(23)

<400>  15
gtatatgatg ggtagttacg gcgn                                              24


<210>  16
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cy5-Anti-serum albumin aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is the fluorescent dye Cy5  or in IUPAC name: 
       6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-y
       l)penta-2,4-dienylidene]indol-1-yl]hexanoic acid

<300>
<302>  Aptamers bound human serum albumin and glycated human serum 
       albumin
<310>  WO 2015/057177 A2
<311>  2013-10-17
<312>  2015-04-23
<313>  (1)..(47)

<400>  16
nagattgcac ttactatctc cacccatatg aattgaatac cctggttt                    48


<210>  17
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cy5-anti-Listeria aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is the fluorescent dye Cy5  or in IUPAC name: 
       6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-y
       l)penta-2,4-dienylidene]indol-1-yl]hexanoic acid

<300>
<301>  Suh SH, Jaykus LA.
<302>  Nucleic acid aptamers for capture and detection of Listeria spp
<303>  Med Res Rev
<304>  167
<305>  4
<306>  454-461
<307>  2013-09-20
<313>  (2)..(46)

<400>  17
natcgatgat ctggtcgccg taacactacc cacatatacg accagg                      46


