                         SEQUENCE LISTING

<110>  Research Institute at Nationwide Children's Hospital
 
<120>  RECOMBINANT ADENO-ASSOCIATED VIRUS PRODUCTS AND METHODS FOR 
       TREATING LIMB GIRDLE MUSCULAR DYSTROPHY 2A

<130>  28335/52684

<150>  US 62/691,934
<151>  2018-06-29

<150>  US 62/865,081
<151>  2019-06-21

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  7629
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  AAVrh.74.tMCK.CAPN3 recombinant genome

<220>
<221>  misc_feature
<222>  (1)..(128)
<223>  AAV2 ITR

<220>
<221>  misc_feature
<222>  (165)..(884)
<223>  tMCK promoter

<220>
<221>  misc_feature
<222>  (937)..(1069)
<223>  chimeric intron

<220>
<221>  misc_feature
<222>  (1101)..(1106)
<223>  Kozak Sequence

<220>
<221>  misc_feature
<222>  (1107)..(3572)
<223>  CAPN3 polynucleotide

<220>
<221>  misc_feature
<222>  (3581)..(3780)
<223>  poly A signal

<220>
<221>  misc_feature
<222>  (3850)..(3977)
<223>  second  AAV2 ITR

<400>  1
gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg       60

tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag      120

gggttcctta atacgactca ctatagggcc gcaccggtaa gcttccacta cgggtctagg      180

ctgcccatgt aaggaggcaa ggcctgggga cacccgagat gcctggttat aattaacccc      240

aacacctgct gccccccccc ccccaacacc tgctgcctga gcctgagcgg ttaccccacc      300

ccggtgcctg ggtcttaggc tctgtacacc atggaggaga agctcgctct aaaaataacc      360

ctgtccctgg tggatccact acgggtctag gctgcccatg taaggaggca aggcctgggg      420

acacccgaga tgcctggtta taattaaccc caacacctgc tgcccccccc cccccaacac      480

ctgctgcctg agcctgagcg gttaccccac cccggtgcct gggtcttagg ctctgtacac      540

catggaggag aagctcgctc taaaaataac cctgtccctg gtggatccac tacgggtcta      600

ggctgcccat gtaaggaggc aaggcctggg gacacccgag atgcctggtt ataattaacc      660

ccaacacctg ctgccccccc ccccccaaca cctgctgcct gagcctgagc ggttacccca      720

ccccggtgcc tgggtcttag gctctgtaca ccatggagga gaagctcgct ctaaaaataa      780

ccctgtccct ggtggatcct ccctggggac agcccctcct ggctagtcac accctgtagg      840

ctcctctata taacccaggg gcacaggggc tgcccccggg tcacggtgga tatccctgca      900

ggtaccacgc gctgtaattg aactgggagt ggacacctgt ggagagaaag gcaaagtgga      960

tgtcagtaag accaataggt gcctatcaga aacgcaagag tcttctctgt ctcgacaagc     1020

ccagtttcta ttggtctcct taaacctgtc ttgtaacctt gatacttacg gagagcaact     1080

gcataagggc tagcctcgag gccaccatgc cgaccgtcat tagcgcatct gtggctccaa     1140

ggacagcggc tgagccccgg tccccagggc cagttcctca cccggcccag agcaaggcca     1200

ctgaggctgg gggtggaaac ccaagtggca tctattcagc catcatcagc cgcaattttc     1260

ctattatcgg agtgaaagag aagacattcg agcaacttca caagaaatgt ctagaaaaga     1320

aagttcttta tgtggaccct gagttcccac cggatgagac ctctctcttt tatagccaga     1380

agttccccat ccagttcgtc tggaagagac ctccggaaat ttgcgagaat ccccgattta     1440

tcattgatgg agccaacaga actgacatct gtcaaggaga gctaggggac tgctggtttc     1500

tcgcagccat tgcctgcctg accctgaacc agcaccttct tttccgagtc ataccccatg     1560

atcaaagttt catcgaaaac tacgcaggga tcttccactt ccagttctgg cgctatggag     1620

agtgggtgga cgtggttata gatgactgcc tgccaacgta caacaatcaa ctggttttca     1680

ccaagtccaa ccaccgcaat gagttctgga gtgctctgct ggagaaggct tatgctaagc     1740

tccatggttc ctacgaagct ctgaaaggtg ggaacaccac agaggccatg gaggacttca     1800

caggaggggt ggcagagttt tttgagatca gggatgctcc tagtgacatg tacaagatca     1860

tgaagaaagc catcgagaga ggctccctca tgggctgctc cattgatgat ggcacgaaca     1920

tgacctatgg aacctctcct tctggtctga acatggggga gttgattgca cggatggtaa     1980

ggaatatgga taactcactg ctccaggact cagacctcga ccccagaggc tcagatgaaa     2040

gaccgacccg gacaatcatt ccggttcagt atgagacaag aatggcctgc gggctggtca     2100

gaggtcacgc ctactctgtc acggggctgg atgaggtccc gttcaaaggt gagaaagtga     2160

agctggtgcg gctgcggaat ccgtggggcc aggtggagtg gaacggttct tggagtgata     2220

gatggaagga ctggagcttt gtggacaaag atgagaaggc ccgtctgcag caccaggtca     2280

ctgaggatgg agagttctgg atgtcctatg aggatttcat ctaccatttc acaaagttgg     2340

agatctgcaa cctcacggcc gatgctctgc agtctgacaa gcttcagacc tggacagtgt     2400

ctgtgaacga gggccgctgg gtacggggtt gctctgccgg aggctgccgc aacttcccag     2460

atactttctg gaccaaccct cagtaccgtc tgaagctcct ggaggaggac gatgaccctg     2520

atgactcgga ggtgatttgc agcttcctgg tggccctgat gcagaagaac cggcggaagg     2580

accggaagct aggggccagt ctcttcacca ttggcttcgc catctacgag gttcccaaag     2640

agatgcacgg gaacaagcag cacctgcaga aggacttctt cctgtacaac gcctccaagg     2700

ccaggagcaa aacctacatc aacatgcggg aggtgtccca gcgcttccgc ctgcctccca     2760

gcgagtacgt catcgtgccc tccacctacg agccccacca ggagggggaa ttcatcctcc     2820

gggtcttctc tgaaaagagg aacctctctg aggaagttga aaataccatc tccgtggatc     2880

ggccagtgaa aaagaaaaaa accaagccca tcatcttcgt ttcggacaga gcaaacagca     2940

acaaggagct gggtgtggac caggagtcag aggagggcaa aggcaaaaca agccctgata     3000

agcaaaagca gtccccacag ccacagcctg gcagctctga tcaggaaagt gaggaacagc     3060

aacaattccg gaacattttc aagcagatag caggagatga catggagatc tgtgcagatg     3120

agctcaagaa ggtccttaac acagtcgtga acaaacacaa ggacctgaag acacacgggt     3180

tcacactgga gtcctgccgt agcatgattg cgctcatgga tacagatggc tctggaaagc     3240

tcaacctgca ggagttccac cacctctgga acaagattaa ggcctggcag aaaattttca     3300

aacactatga cacagaccag tccggcacca tcaacagcta cgagatgcga aatgcagtca     3360

acgacgcagg attccacctc aacaaccagc tctatgacat cattaccatg cggtacgcag     3420

acaaacacat gaacatcgac tttgacagtt tcatctgctg cttcgttagg ctggagggca     3480

tgttcagagc ttttcatgca tttgacaagg atggagatgg tatcatcaag ctcaacgttc     3540

tggagtggct gcagctcacc atgtatgcct gagcggccgc ggggatccag acatgataag     3600

atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg     3660

taaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa     3720

caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggttttttc     3780

ggatcctcta gagtcgacca gagcatggct acgtagataa gtagcatggc gggttaatca     3840

ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc     3900

tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag     3960

tgagcgagcg agcgcgcagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt     4020

gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct     4080

gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga     4140

taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc     4200

cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg     4260

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     4320

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     4380

tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     4440

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     4500

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     4560

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     4620

cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct     4680

gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac     4740

cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc     4800

tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg     4860

ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta     4920

aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa aaatattccg gaattgccag     4980

ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc     5040

cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt     5100

ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc     5160

tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc     5220

tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg     5280

aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag     5340

ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg     5400

ggcaggatct cctgtcatcc caccttgctc ctgccgagaa agtatccatc atggctgatg     5460

caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac     5520

atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg     5580

acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc     5640

ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg     5700

aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc     5760

aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc     5820

gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc     5880

ttcttgacga gttcttctga accggtaata ttattgaagc atttatcagg gttattgtct     5940

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     6000

atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta     6060

taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa     6120

cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag     6180

cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta     6240

tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag     6300

atgcgtaagg agaaaatacc gcatcaggaa cttccaacat ccaataaatc atacaggcaa     6360

ggcaaagaat tagcaaaatt aagcaataaa gcctcagagc ataaagctaa atcggttgta     6420

ccaaaaacat tatgaccctg taatactttt gcgggagaag cctttatttc aacgcaagga     6480

taaaaatttt tagaaccctc atatatttta aatgcaatgc ctgagtaatg tgtaggtaaa     6540

gattcaaacg ggtgagaaag gccggagaca gtcaaatcac catcaatatg atattcaacc     6600

gttctagctg ataaattcat gccggagagg gtagctattt ttgagaggtc tctacaaagg     6660

ctatcaggtc attgcctgag agtctggagc aaacaagaga atcgatgaac ggtaatcgta     6720

aaactagcat gtcaatcata tgtaccccgg ttgataatca gaaaagcccc aaaaacagga     6780

agattgtata agcaaatatt taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa     6840

atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata     6900

aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac     6960

tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc     7020

cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaatcactaa     7080

atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg     7140

cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg     7200

tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtact     7260

atggttgctt tgacgagcac gtataacgtg ctttcctcgt tagaatcaga gcgggagcta     7320

aacaggaggc cgattaaagg gattttagac aggaacggta cgccagaatc ctgagaagtg     7380

tttttataat cagtgaggcc accgagtaaa agagtctgtc catcacgcaa attaaccgtt     7440

gtcgcaatac ttctttgatt agtaataaca tcacttgcct gagtagaaga actcaaacta     7500

tcggccttgc tggtaatatc cagaacaata ttaccgccag ccattgcaac ggaatcgcca     7560

ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     7620

acgccagct                                                             7629


<210>  2
<211>  2466
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  CAPN3 polynucleotide

<400>  2
atgccgaccg tcattagcgc atctgtggct ccaaggacag cggctgagcc ccggtcccca       60

gggccagttc ctcacccggc ccagagcaag gccactgagg ctgggggtgg aaacccaagt      120

ggcatctatt cagccatcat cagccgcaat tttcctatta tcggagtgaa agagaagaca      180

ttcgagcaac ttcacaagaa atgtctagaa aagaaagttc tttatgtgga ccctgagttc      240

ccaccggatg agacctctct cttttatagc cagaagttcc ccatccagtt cgtctggaag      300

agacctccgg aaatttgcga gaatccccga tttatcattg atggagccaa cagaactgac      360

atctgtcaag gagagctagg ggactgctgg tttctcgcag ccattgcctg cctgaccctg      420

aaccagcacc ttcttttccg agtcataccc catgatcaaa gtttcatcga aaactacgca      480

gggatcttcc acttccagtt ctggcgctat ggagagtggg tggacgtggt tatagatgac      540

tgcctgccaa cgtacaacaa tcaactggtt ttcaccaagt ccaaccaccg caatgagttc      600

tggagtgctc tgctggagaa ggcttatgct aagctccatg gttcctacga agctctgaaa      660

ggtgggaaca ccacagaggc catggaggac ttcacaggag gggtggcaga gttttttgag      720

atcagggatg ctcctagtga catgtacaag atcatgaaga aagccatcga gagaggctcc      780

ctcatgggct gctccattga tgatggcacg aacatgacct atggaacctc tccttctggt      840

ctgaacatgg gggagttgat tgcacggatg gtaaggaata tggataactc actgctccag      900

gactcagacc tcgaccccag aggctcagat gaaagaccga cccggacaat cattccggtt      960

cagtatgaga caagaatggc ctgcgggctg gtcagaggtc acgcctactc tgtcacgggg     1020

ctggatgagg tcccgttcaa aggtgagaaa gtgaagctgg tgcggctgcg gaatccgtgg     1080

ggccaggtgg agtggaacgg ttcttggagt gatagatgga aggactggag ctttgtggac     1140

aaagatgaga aggcccgtct gcagcaccag gtcactgagg atggagagtt ctggatgtcc     1200

tatgaggatt tcatctacca tttcacaaag ttggagatct gcaacctcac ggccgatgct     1260

ctgcagtctg acaagcttca gacctggaca gtgtctgtga acgagggccg ctgggtacgg     1320

ggttgctctg ccggaggctg ccgcaacttc ccagatactt tctggaccaa ccctcagtac     1380

cgtctgaagc tcctggagga ggacgatgac cctgatgact cggaggtgat ttgcagcttc     1440

ctggtggccc tgatgcagaa gaaccggcgg aaggaccgga agctaggggc cagtctcttc     1500

accattggct tcgccatcta cgaggttccc aaagagatgc acgggaacaa gcagcacctg     1560

cagaaggact tcttcctgta caacgcctcc aaggccagga gcaaaaccta catcaacatg     1620

cgggaggtgt cccagcgctt ccgcctgcct cccagcgagt acgtcatcgt gccctccacc     1680

tacgagcccc accaggaggg ggaattcatc ctccgggtct tctctgaaaa gaggaacctc     1740

tctgaggaag ttgaaaatac catctccgtg gatcggccag tgaaaaagaa aaaaaccaag     1800

cccatcatct tcgtttcgga cagagcaaac agcaacaagg agctgggtgt ggaccaggag     1860

tcagaggagg gcaaaggcaa aacaagccct gataagcaaa agcagtcccc acagccacag     1920

cctggcagct ctgatcagga aagtgaggaa cagcaacaat tccggaacat tttcaagcag     1980

atagcaggag atgacatgga gatctgtgca gatgagctca agaaggtcct taacacagtc     2040

gtgaacaaac acaaggacct gaagacacac gggttcacac tggagtcctg ccgtagcatg     2100

attgcgctca tggatacaga tggctctgga aagctcaacc tgcaggagtt ccaccacctc     2160

tggaacaaga ttaaggcctg gcagaaaatt ttcaaacact atgacacaga ccagtccggc     2220

accatcaaca gctacgagat gcgaaatgca gtcaacgacg caggattcca cctcaacaac     2280

cagctctatg acatcattac catgcggtac gcagacaaac acatgaacat cgactttgac     2340

agtttcatct gctgcttcgt taggctggag ggcatgttca gagcttttca tgcatttgac     2400

aaggatggag atggtatcat caagctcaac gttctggagt ggctgcagct caccatgtat     2460

gcctga                                                                2466


<210>  3
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  tMCK promoter

<400>  3
ccactacggg tctaggctgc ccatgtaagg aggcaaggcc tggggacacc cgagatgcct       60

ggttataatt aaccccaaca cctgctgccc cccccccccc aacacctgct gcctgagcct      120

gagcggttac cccaccccgg tgcctgggtc ttaggctctg tacaccatgg aggagaagct      180

cgctctaaaa ataaccctgt ccctggtgga tccactacgg gtctaggctg cccatgtaag      240

gaggcaaggc ctggggacac ccgagatgcc tggttataat taaccccaac acctgctgcc      300

cccccccccc caacacctgc tgcctgagcc tgagcggtta ccccaccccg gtgcctgggt      360

cttaggctct gtacaccatg gaggagaagc tcgctctaaa aataaccctg tccctggtgg      420

atccactacg ggtctaggct gcccatgtaa ggaggcaagg cctggggaca cccgagatgc      480

ctggttataa ttaaccccaa cacctgctgc cccccccccc ccaacacctg ctgcctgagc      540

ctgagcggtt accccacccc ggtgcctggg tcttaggctc tgtacaccat ggaggagaag      600

ctcgctctaa aaataaccct gtccctggtg gatcctccct ggggacagcc cctcctggct      660

agtcacaccc tgtaggctcc tctatataac ccaggggcac aggggctgcc cccgggtcac      720


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  MCK forward primer

<400>  4
cccgagatgc ctggttataa tt                                                22


<210>  5
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  MCK reverse primer

<400>  5
gctcaggcag caggtgttg                                                    19


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  MCK probe

<400>  6
ccagacatgt ggctgctccc cc                                                22


<210>  7
<211>  821
<212>  PRT
<213>  Homo Sapiens

<400>  7

Met Pro Thr Val Ile Ser Ala Ser Val Ala Pro Arg Thr Ala Ala Glu 
1               5                   10                  15      


Pro Arg Ser Pro Gly Pro Val Pro His Pro Ala Gln Ser Lys Ala Thr 
            20                  25                  30          


Glu Ala Gly Gly Gly Asn Pro Ser Gly Ile Tyr Ser Ala Ile Ile Ser 
        35                  40                  45              


Arg Asn Phe Pro Ile Ile Gly Val Lys Glu Lys Thr Phe Glu Gln Leu 
    50                  55                  60                  


His Lys Lys Cys Leu Glu Lys Lys Val Leu Tyr Val Asp Pro Glu Phe 
65                  70                  75                  80  


Pro Pro Asp Glu Thr Ser Leu Phe Tyr Ser Gln Lys Phe Pro Ile Gln 
                85                  90                  95      


Phe Val Trp Lys Arg Pro Pro Glu Ile Cys Glu Asn Pro Arg Phe Ile 
            100                 105                 110         


Ile Asp Gly Ala Asn Arg Thr Asp Ile Cys Gln Gly Glu Leu Gly Asp 
        115                 120                 125             


Cys Trp Phe Leu Ala Ala Ile Ala Cys Leu Thr Leu Asn Gln His Leu 
    130                 135                 140                 


Leu Phe Arg Val Ile Pro His Asp Gln Ser Phe Ile Glu Asn Tyr Ala 
145                 150                 155                 160 


Gly Ile Phe His Phe Gln Phe Trp Arg Tyr Gly Glu Trp Val Asp Val 
                165                 170                 175     


Val Ile Asp Asp Cys Leu Pro Thr Tyr Asn Asn Gln Leu Val Phe Thr 
            180                 185                 190         


Lys Ser Asn His Arg Asn Glu Phe Trp Ser Ala Leu Leu Glu Lys Ala 
        195                 200                 205             


Tyr Ala Lys Leu His Gly Ser Tyr Glu Ala Leu Lys Gly Gly Asn Thr 
    210                 215                 220                 


Thr Glu Ala Met Glu Asp Phe Thr Gly Gly Val Ala Glu Phe Phe Glu 
225                 230                 235                 240 


Ile Arg Asp Ala Pro Ser Asp Met Tyr Lys Ile Met Lys Lys Ala Ile 
                245                 250                 255     


Glu Arg Gly Ser Leu Met Gly Cys Ser Ile Asp Asp Gly Thr Asn Met 
            260                 265                 270         


Thr Tyr Gly Thr Ser Pro Ser Gly Leu Asn Met Gly Glu Leu Ile Ala 
        275                 280                 285             


Arg Met Val Arg Asn Met Asp Asn Ser Leu Leu Gln Asp Ser Asp Leu 
    290                 295                 300                 


Asp Pro Arg Gly Ser Asp Glu Arg Pro Thr Arg Thr Ile Ile Pro Val 
305                 310                 315                 320 


Gln Tyr Glu Thr Arg Met Ala Cys Gly Leu Val Arg Gly His Ala Tyr 
                325                 330                 335     


Ser Val Thr Gly Leu Asp Glu Val Pro Phe Lys Gly Glu Lys Val Lys 
            340                 345                 350         


Leu Val Arg Leu Arg Asn Pro Trp Gly Gln Val Glu Trp Asn Gly Ser 
        355                 360                 365             


Trp Ser Asp Arg Trp Lys Asp Trp Ser Phe Val Asp Lys Asp Glu Lys 
    370                 375                 380                 


Ala Arg Leu Gln His Gln Val Thr Glu Asp Gly Glu Phe Trp Met Ser 
385                 390                 395                 400 


Tyr Glu Asp Phe Ile Tyr His Phe Thr Lys Leu Glu Ile Cys Asn Leu 
                405                 410                 415     


Thr Ala Asp Ala Leu Gln Ser Asp Lys Leu Gln Thr Trp Thr Val Ser 
            420                 425                 430         


Val Asn Glu Gly Arg Trp Val Arg Gly Cys Ser Ala Gly Gly Cys Arg 
        435                 440                 445             


Asn Phe Pro Asp Thr Phe Trp Thr Asn Pro Gln Tyr Arg Leu Lys Leu 
    450                 455                 460                 


Leu Glu Glu Asp Asp Asp Pro Asp Asp Ser Glu Val Ile Cys Ser Phe 
465                 470                 475                 480 


Leu Val Ala Leu Met Gln Lys Asn Arg Arg Lys Asp Arg Lys Leu Gly 
                485                 490                 495     


Ala Ser Leu Phe Thr Ile Gly Phe Ala Ile Tyr Glu Val Pro Lys Glu 
            500                 505                 510         


Met His Gly Asn Lys Gln His Leu Gln Lys Asp Phe Phe Leu Tyr Asn 
        515                 520                 525             


Ala Ser Lys Ala Arg Ser Lys Thr Tyr Ile Asn Met Arg Glu Val Ser 
    530                 535                 540                 


Gln Arg Phe Arg Leu Pro Pro Ser Glu Tyr Val Ile Val Pro Ser Thr 
545                 550                 555                 560 


Tyr Glu Pro His Gln Glu Gly Glu Phe Ile Leu Arg Val Phe Ser Glu 
                565                 570                 575     


Lys Arg Asn Leu Ser Glu Glu Val Glu Asn Thr Ile Ser Val Asp Arg 
            580                 585                 590         


Pro Val Lys Lys Lys Lys Thr Lys Pro Ile Ile Phe Val Ser Asp Arg 
        595                 600                 605             


Ala Asn Ser Asn Lys Glu Leu Gly Val Asp Gln Glu Ser Glu Glu Gly 
    610                 615                 620                 


Lys Gly Lys Thr Ser Pro Asp Lys Gln Lys Gln Ser Pro Gln Pro Gln 
625                 630                 635                 640 


Pro Gly Ser Ser Asp Gln Glu Ser Glu Glu Gln Gln Gln Phe Arg Asn 
                645                 650                 655     


Ile Phe Lys Gln Ile Ala Gly Asp Asp Met Glu Ile Cys Ala Asp Glu 
            660                 665                 670         


Leu Lys Lys Val Leu Asn Thr Val Val Asn Lys His Lys Asp Leu Lys 
        675                 680                 685             


Thr His Gly Phe Thr Leu Glu Ser Cys Arg Ser Met Ile Ala Leu Met 
    690                 695                 700                 


Asp Thr Asp Gly Ser Gly Lys Leu Asn Leu Gln Glu Phe His His Leu 
705                 710                 715                 720 


Trp Asn Lys Ile Lys Ala Trp Gln Lys Ile Phe Lys His Tyr Asp Thr 
                725                 730                 735     


Asp Gln Ser Gly Thr Ile Asn Ser Tyr Glu Met Arg Asn Ala Val Asn 
            740                 745                 750         


Asp Ala Gly Phe His Leu Asn Asn Gln Leu Tyr Asp Ile Ile Thr Met 
        755                 760                 765             


Arg Tyr Ala Asp Lys His Met Asn Ile Asp Phe Asp Ser Phe Ile Cys 
    770                 775                 780                 


Cys Phe Val Arg Leu Glu Gly Met Phe Arg Ala Phe His Ala Phe Asp 
785                 790                 795                 800 


Lys Asp Gly Asp Gly Ile Ile Lys Leu Asn Val Leu Glu Trp Leu Gln 
                805                 810                 815     


Leu Thr Met Tyr Ala 
            820     


<210>  8
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  8
cggagagcaa ctgcataag                                                    19


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  9
ggctgatgat ggctgaatag                                                   20


