                         SEQUENCE LISTING

<110>  The University of North Carolina at Chapel Hill
       Lincoln University
       Gray, Steven James
       Palmer, David
       Mitchell, Nadia
 
<120>  Optimized CLN5 Genes and Expression Cassettes and Their Use

<130>  5470-853WO

<150>  US 62/828,717
<151>  2019-04-03

<150>  US 62/691,359
<151>  2018-06-28

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1077
<212>  DNA
<213>  Artificial

<220>
<223>  human CLN5 ORF

<400>  1
atggcgcagg aggtggacac tgcacagggg gccgagatga gaagaggagc cggagcagct       60

agaggacggg ccagctggtg ttgggccttg gcgctgctct ggctggctgt ggtgcctggc      120

tggtccagag tgtccgggat tccctcgagg cgccattggc ctgtgccata taagcgcttc      180

gacttccggc ctaagcccga cccgtactgc caggccaagt acaccttttg tcccaccgga      240

tcgcccatcc ctgtgatgga gggcgacgat gacatcgaag tgttccgact gcaagccccc      300

gtgtgggaat tcaaatacgg cgaccttctg ggccacctga agatcatgca tgacgccatc      360

gggttccgct cgaccctgac cggaaagaac tacaccatgg agtggtacga actgttccag      420

ctgggaaact gcaccttccc tcaccttcgg cctgaaatgg atgcaccttt ctggtgcaac      480

caaggcgctg cctgcttctt tgaggggatc gacgatgtgc actggaagga gaacggaacc      540

ctggtccaag tggccactat ctccgggaac atgttcaacc agatggccaa atgggtgaag      600

caggataatg agactggcat ctactatgaa acttggaacg tgaaagccag cccggagaag      660

ggtgccgaaa cctggttcga ttcctacgac tgctccaagt tcgtgctgcg gaccttcaac      720

aagctggccg agttcggtgc cgagttcaag aacattgaaa ccaactacac tcgcattttc      780

ctctactcgg gcgaacccac ctacctggga aacgaaacta gcgtctttgg gccgactgga      840

aacaagactt tggggctcgc gatcaagagg ttctactacc ctttcaagcc gcaccttccg      900

accaaggaat tcttgctgag cctgctgcag attttcgatg ccgtgatcgt gcataagcag      960

ttctatctgt tttacaattt cgaatactgg ttcctcccga tgaagttccc gttcattaag     1020

atcacctacg aggaaatccc actcccgatc cgcaacaaga ccctgtccgg tctgtaa        1077


<210>  2
<211>  1089
<212>  DNA
<213>  Artificial

<220>
<223>  ovine CLN5 ORF

<400>  2
atggcacaag caggaggagc aggggcagga gcttggggaa ggagaggagc aggagctgga       60

gcgggccctg agcgggcccc ttggcgctgg gccccggccc tgctgtggct cgcggcggct      120

actgccgctg ccgccgccgc cggggacccg tcccggagac agtggccggt gccatacaag      180

cgcttcagct ttcggcccga gccggacccg tactgtcagg ccaagtacac cttctgccca      240

accggctccc ctatcccggt catgaaggac gacgacgtca ttgaagtgtt caggctccaa      300

gcccccgtgt gggagttcaa atacggagat ctgcttggtc acttgaagat tatgcacgat      360

gcgatcggct ttcgctccac cctgaccgag aagaactaca ctatggagtg gtacgaattg      420

ttccagctgg gcaactgcac tttcccccac ctgaggcccg agatgaacgc gcctttctgg      480

tgtaaccagg gtgcagcctg ctttttcgag ggcatcgacg acaaccattg gaaggagaac      540

ggcaccctgg tcctggtggc cacaatcagc ggcgggatgt tcaacaagat ggccaaatgg      600

gtgaagcagg acaacgagac tggaatctat tacgagactt ggaccgtgca agcctcaccc      660

aagaaggagg ccgagaagtg gttcgagtcc tacgactgct caaagttcgt gctgcgcacc      720

tacgaaaagc tggccgaact tggagccgac ttcaagaaga tcgaaaccaa ctacacccgc      780

atctttctgt attcgggcga acccacgtac ctgggaaacg aaacctccgt gttcggccct      840

accgggaaca agactcttgc tctcgccatc aagaagttct actacccgtt caagccgcac      900

ctgtcgacca aggaattcct cctgagcctg ctgcagatct ttgatgcggt ggtcattcac      960

cgggaattct atctgttcta caatttcgag tactggttcc tccctatgaa atccccattc     1020

attaagatca cttacgaaga aattccgctc cccaatcgca aaaaccggac cctgtccggt     1080

ctgtgataa                                                             1089


<210>  3
<211>  306
<212>  DNA
<213>  Artificial

<220>
<223>  chicken beta actin promoter

<400>  3
tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc       60

cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt      120

gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag      180

gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga      240

aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg      300

cgggcg                                                                 306


<210>  4
<211>  137
<212>  DNA
<213>  Artificial

<220>
<223>  Chimeric intron

<400>  4
ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc       60

gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc      120

tccgggctgt aattagc                                                     137


<210>  5
<211>  1881
<212>  DNA
<213>  Artificial

<220>
<223>  CAGGS promoter

<400>  5
gatctgaatt cggatcttca atattggcca ttagccatat tattcattgg ttatatagca       60

taaatcaata ttggatattg gccattgcat acgttgtatc tatatcataa tatgtacatt      120

tatattggct catgtccaat atgaccgcca tgttggcatt gattattgac tagttattaa      180

tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa      240

cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata      300

atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag      360

tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc      420

cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta      480

cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag      540

gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg      600

tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc      660

gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg      720

gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg      780

cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgacgct gccttcgccc      840

cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact      900

cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta      960

atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc     1020

tttgtgcggg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg     1080

cgtgcggccc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc     1140

gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg     1200

ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtat     1260

gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg     1320

gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg     1380

gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct     1440

cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca     1500

gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttactttg tcccaaatct     1560

gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc     1620

ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc     1680

gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg     1740

gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta     1800

accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc     1860

tgtctcatca ttttggcaaa g                                               1881


<210>  6
<211>  256
<212>  DNA
<213>  Artificial

<220>
<223>  CMV enhancer

<400>  6
tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac       60

gtcaatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      120

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      180

caatgacggt aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc      240

tacttggcag tacatc                                                      256


<210>  7
<211>  48
<212>  DNA
<213>  Artificial

<220>
<223>  SpA

<400>  7
aataaagagc tcagatgcat cgatcagagt gtgttggttt tttgtgtg                    48


<210>  8
<211>  143
<212>  DNA
<213>  Artificial

<220>
<223>  SV40pA

<400>  8
agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa       60

atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa      120

taaacaagtt aacaacaaca att                                              143


<210>  9
<211>  254
<212>  DNA
<213>  Artificial

<220>
<223>  BGHpA

<400>  9
ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt       60

gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca      120

ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga      180

ggattgggaa gacaacagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc      240

ggaaagaacc agct                                                        254


<210>  10
<211>  2176
<212>  DNA
<213>  Artificial

<220>
<223>  Human CLN5 cassette

<400>  10
tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac       60

gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg      120

ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag      180

tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat      240

gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat      300

ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc      360

aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg      420

ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg      480

tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg      540

gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgacgctgcc      600

ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg      660

cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga      720

gcaagaggta agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg      780

agcacctgcc tgaaatcact ttttttcagg ttggaccggt cgccaccatg gcgcaggagg      840

tggacactgc acagggggcc gagatgagaa gaggagccgg agcagctaga ggacgggcca      900

gctggtgttg ggccttggcg ctgctctggc tggctgtggt gcctggctgg tccagagtgt      960

ccgggattcc ctcgaggcgc cattggcctg tgccatataa gcgcttcgac ttccggccta     1020

agcccgaccc gtactgccag gccaagtaca ccttttgtcc caccggatcg cccatccctg     1080

tgatggaggg cgacgatgac atcgaagtgt tccgactgca agcccccgtg tgggaattca     1140

aatacggcga ccttctgggc cacctgaaga tcatgcatga cgccatcggg ttccgctcga     1200

ccctgaccgg aaagaactac accatggagt ggtacgaact gttccagctg ggaaactgca     1260

ccttccctca ccttcggcct gaaatggatg cacctttctg gtgcaaccaa ggcgctgcct     1320

gcttctttga ggggatcgac gatgtgcact ggaaggagaa cggaaccctg gtccaagtgg     1380

ccactatctc cgggaacatg ttcaaccaga tggccaaatg ggtgaagcag gataatgaga     1440

ctggcatcta ctatgaaact tggaacgtga aagccagccc ggagaagggt gccgaaacct     1500

ggttcgattc ctacgactgc tccaagttcg tgctgcggac cttcaacaag ctggccgagt     1560

tcggtgccga gttcaagaac attgaaacca actacactcg cattttcctc tactcgggcg     1620

aacccaccta cctgggaaac gaaactagcg tctttgggcc gactggaaac aagactttgg     1680

ggctcgcgat caagaggttc tactaccctt tcaagccgca ccttccgacc aaggaattct     1740

tgctgagcct gctgcagatt ttcgatgccg tgatcgtgca taagcagttc tatctgtttt     1800

acaatttcga atactggttc ctcccgatga agttcccgtt cattaagatc acctacgagg     1860

aaatcccact cccgatccgc aacaagaccc tgtccggtct gtaatagcgg ccgcgcggat     1920

ccctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc     1980

ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg     2040

cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg     2100

gaggattggg aagacaacag caggcatgct ggggatgcgg tgggctctat ggcttctgag     2160

gcggaaagaa ccagct                                                     2176


<210>  11
<211>  2185
<212>  DNA
<213>  Artificial

<220>
<223>  Ovine CLN5 cassette

<400>  11
tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac       60

gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg      120

ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag      180

tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat      240

gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat      300

ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc      360

aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg      420

ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg      480

tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg      540

gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgacgctgcc      600

ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg      660

cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga      720

gcaagaggta agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg      780

agcacctgcc tgaaatcact ttttttcagg ttggaccggt cgccaccatg gcacaagcag      840

gaggagcagg ggcaggagct tggggaagga gaggagcagg agctggagcg ggccctgagc      900

gggccccttg gcgctgggcc ccggccctgc tgtggctcgc ggcggctact gccgctgccg      960

ccgccgccgg ggacccgtcc cggagacagt ggccggtgcc atacaagcgc ttcagctttc     1020

ggcccgagcc ggacccgtac tgtcaggcca agtacacctt ctgcccaacc ggctccccta     1080

tcccggtcat gaaggacgac gacgtcattg aagtgttcag gctccaagcc cccgtgtggg     1140

agttcaaata cggagatctg cttggtcact tgaagattat gcacgatgcg atcggctttc     1200

gctccaccct gaccgagaag aactacacta tggagtggta cgaattgttc cagctgggca     1260

actgcacttt cccccacctg aggcccgaga tgaacgcgcc tttctggtgt aaccagggtg     1320

cagcctgctt tttcgagggc atcgacgaca accattggaa ggagaacggc accctggtcc     1380

tggtggccac aatcagcggc gggatgttca acaagatggc caaatgggtg aagcaggaca     1440

acgagactgg aatctattac gagacttgga ccgtgcaagc ctcacccaag aaggaggccg     1500

agaagtggtt cgagtcctac gactgctcaa agttcgtgct gcgcacctac gaaaagctgg     1560

ccgaacttgg agccgacttc aagaagatcg aaaccaacta cacccgcatc tttctgtatt     1620

cgggcgaacc cacgtacctg ggaaacgaaa cctccgtgtt cggccctacc gggaacaaga     1680

ctcttgctct cgccatcaag aagttctact acccgttcaa gccgcacctg tcgaccaagg     1740

aattcctcct gagcctgctg cagatctttg atgcggtggt cattcaccgg gaattctatc     1800

tgttctacaa tttcgagtac tggttcctcc ctatgaaatc cccattcatt aagatcactt     1860

acgaagaaat tccgctcccc aatcgcaaaa accggaccct gtccggtctg tgataacggc     1920

cgcgcggatc cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc     1980

gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa     2040

attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac     2100

agcaaggggg aggattggga agacaacagc aggcatgctg gggatgcggt gggctctatg     2160

gcttctgagg cggaaagaac cagct                                           2185


