                         SEQUENCE LISTING

<110>  LUCENCE LIFE SCIENCES PTE. LTD.
 
<120>  METHOD FOR DETECTION AND QUANTIFICATION OF GENETIC ALTERATIONS

<130>  36425SG22

<150>  10201805450Y
<151>  2018-06-25

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  barcode sequence


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  n is a, c, g, or t

<400>  1
nnnnnnnnnn                                                              10


<210>  2
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an example of the barcode sequence

<400>  2
cattacatac                                                              10


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an exemple of the barcode sequence

<400>  3
gcgtggacaa                                                              10


<210>  4
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an example of the barcode sequence

<400>  4
tttttagaca                                                              10


<210>  5
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an example of the barcode sequence

<400>  5
taagaggtcc                                                              10


<210>  6
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an example of a "primer" when the target sequence is EGFR-exon 18
       1 (an example of primer A, illustrated in Figure 1a)


<220>
<221>  misc_feature
<222>  (21)..(30)
<223>  n is a, c, g, or t

<400>  6
acacgacgct cttccgatct nnnnnnnnnn ggtgaccctt gtctctgtgt tc               52


<210>  7
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an example of subsequent primers for the "completion of amplicon"
       (an example of primer C, illustrated in Figure 1c)

<400>  7
gacgtgtgct cttccgatct gagcccagca ctttgatctt ttt                         43


<210>  8
<211>  178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  one example of expected amplicon (only target-specific region)

<400>  8
ggtgaccctt gtctctgtgt tcgagcccag cactttgatc tttttggtga cccttgtctc       60

tgtgttcttg tcccccccag cttgtggagc ctcttacacc cagtggagaa gctcccaacc      120

aagctctctt gaggatcttg aaggaaactg aattcaaaaa gatcaaagtg ctgggctc        178


<210>  9
<211>  183
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an example of the product after amplicon completion (in two 
       steps) (only one strand of the double stranded product is shown)


<220>
<221>  misc_feature
<222>  (21)..(30)
<223>  n is a, c, g, or t

<400>  9
acacgacgct cttccgatct nnnnnnnnnn ggtgaccctt gtctctgtgt tcttgtcccc       60

cccagcttgt ggagcctctt acacccagtg gagaagctcc caaccaagct ctcttgagga      120

tcttgaagga aactgaattc aaaaagatca aagtgctggg ctcagatcgg aagagcacac      180

gtc                                                                    183


<210>  10
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universal amplification primer 1 (an example of the primer for 
       amplifying product C or D)

<400>  10
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct         58


<210>  11
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universal amplification primer 2 (indexed) (an example of the 
       primer for amplifying product C or D, the index is the bases in 
       bold and italic font)

<400>  11
caagcagaag acggcatacg agatatcacg gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  12
<211>  265
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an example of the final product (suitable for sequencing on 
       Illumina)


<220>
<221>  misc_feature
<222>  (59)..(68)
<223>  n is a, c, g, or t

<400>  12
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctnn       60

nnnnnnnngg tgacccttgt ctctgtgttc ttgtcccccc cagcttgtgg agcctcttac      120

acccagtgga gaagctccca accaagctct cttgaggatc ttgaaggaaa ctgaattcaa      180

aaagatcaaa gtgctgggct cagatcggaa gagcacacgt ctgaactcca gtcaccgtga      240

tatctcgtat gccgtcttct gcttg                                            265


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a universal P5 adapter

<400>  13
aatgatacgg cgaccaccga gatct                                             25


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  an indexed P7 adapter

<400>  14
caagcagaag acggcatacg agat                                              24


<210>  15
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID1

<400>  15
caagcagaag acggcatacg agatatcacg gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  16
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID2

<400>  16
caagcagaag acggcatacg agatcgatgt gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  17
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID3

<400>  17
caagcagaag acggcatacg agatttaggc gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  18
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID4

<400>  18
caagcagaag acggcatacg agattgacca gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  19
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID5

<400>  19
caagcagaag acggcatacg agatacagtg gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  20
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID6

<400>  20
caagcagaag acggcatacg agatgccaat gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  21
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID7

<400>  21
caagcagaag acggcatacg agatcagatc gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  22
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID8

<400>  22
caagcagaag acggcatacg agatacttga gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  23
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID9

<400>  23
caagcagaag acggcatacg agatgatcag gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  24
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID10

<400>  24
caagcagaag acggcatacg agattagctt gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  25
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID11

<400>  25
caagcagaag acggcatacg agatggctac gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  26
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID12

<400>  26
caagcagaag acggcatacg agatcttgta gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  27
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID13

<400>  27
caagcagaag acggcatacg agatagtcaa gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  28
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of P7 adapter and sample index, P7 adapter R-ID14

<400>  28
caagcagaag acggcatacg agatagttcc gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


