                         б

<110>  ǺҽҩƼ޹˾
 
<120>  PD-L1϶ļ;

<130>  P2019TC763

<150>  201810696663.X
<151>  2018-06-29

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109

<400>  1

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Asp Asp Ser 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val Cys Gln Leu Val 
        35                  40                  45              


Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser His Asp Arg Ala Lys Asn Thr Ile Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr Cys Glu Gly Asp 
            100                 105                 110         


Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  2
<211>  142
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-chis

<400>  2

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Asp Asp Ser 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val Cys Gln Leu Val 
        35                  40                  45              


Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser His Asp Arg Ala Lys Asn Thr Ile Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr Cys Glu Gly Asp 
            100                 105                 110         


Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Ser 
        115                 120                 125             


Met Asp Pro Gly Gly Ser His His His His His His His His 
    130                 135                 140         


<210>  3
<211>  381
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  109ĺ

<400>  3
caggtgcagc tgcaggagtc tggaggaggc tcggtgcagg ctggggggtc tctgagactc       60

tcctgtacag cctctggatt cagtttagat gattctgaca tgggctggta ccgccaggct      120

cgtgggaatg tgtgccagtt ggtgtcaaca attgctagtg atagaagtac atactataca      180

ccctccgtga agggccgatt caccatctcc catgacagag ccaagaacac aatttatctg      240

caaatgaaca gcctgaaacc tgaggacacg gccgtgtatt actgtgcggc agcccctcgc      300

ctggcctaca caacggcgat gacgtgcgag ggggactttg cttactgggg ccagggaacc      360

caggtcaccg tctcctcata a                                                381


<210>  4
<211>  429
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  109-chisĺ

<400>  4
caggtgcagc tgcaggagtc tggaggaggc tcggtgcagg ctggggggtc tctgagactc       60

tcctgtacag cctctggatt cagtttagat gattctgaca tgggctggta ccgccaggct      120

cgtgggaatg tgtgccagtt ggtgtcaaca attgctagtg atagaagtac atactataca      180

ccctccgtga agggccgatt caccatctcc catgacagag ccaagaacac aatttatctg      240

caaatgaaca gcctgaaacc tgaggacacg gccgtgtatt actgtgcggc agcccctcgc      300

ctggcctaca caacggcgat gacgtgcgag ggggactttg cttactgggg ccagggaacc      360

caggtcaccg tctcctcagg cagcatggat cctggaggat ctcatcatca ccaccaccat      420

catcactaa                                                              429


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1

<400>  5

Gly Phe Ser Leu Asp Asp Ser Asp Met Gly 
1               5                   10  


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2

<400>  6

Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Lys Gly 
1               5                   10                  15  


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3

<400>  7

Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr Cys Glu Gly Asp Phe 
1               5                   10                  15      


Ala Tyr 
        


<210>  8
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-K86R&P87A-cHis

<400>  8

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val 
            20                  25                  30          


Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser 
        35                  40                  45              


Leu Asp Asp Ser Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val 
    50                  55                  60                  


Cys Gln Leu Val Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr 
65                  70                  75                  80  


Pro Ser Val Lys Gly Arg Phe Thr Ile Ser His Asp Arg Ala Lys Asn 
                85                  90                  95      


Thr Ile Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr 
        115                 120                 125             


Cys Glu Gly Asp Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Ser Met Asp Pro Gly Gly Ser His His His His His His 
145                 150                 155                 160 


His His 
        


<210>  9
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-RDNSE-cHis

<400>  9

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val 
            20                  25                  30          


Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser 
        35                  40                  45              


Leu Asp Asp Ser Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val 
    50                  55                  60                  


Cys Gln Leu Val Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr 
65                  70                  75                  80  


Pro Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Glu Asn 
                85                  90                  95      


Thr Ile Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr 
        115                 120                 125             


Cys Glu Gly Asp Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Ser Met Asp Pro Gly Gly Ser His His His His His His 
145                 150                 155                 160 


His His 
        


<210>  10
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-K64Q-cHis

<400>  10

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val 
            20                  25                  30          


Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser 
        35                  40                  45              


Leu Asp Asp Ser Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val 
    50                  55                  60                  


Cys Gln Leu Val Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr 
65                  70                  75                  80  


Pro Ser Val Gln Gly Arg Phe Thr Ile Ser His Asp Arg Ala Lys Asn 
                85                  90                  95      


Thr Ile Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr 
        115                 120                 125             


Cys Glu Gly Asp Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Ser Met Asp Pro Gly Gly Ser His His His His His His 
145                 150                 155                 160 


His His 
        


<210>  11
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-R73N&K75E-cHis

<400>  11

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val 
            20                  25                  30          


Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser 
        35                  40                  45              


Leu Asp Asp Ser Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val 
    50                  55                  60                  


Cys Gln Leu Val Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr 
65                  70                  75                  80  


Pro Ser Val Lys Gly Arg Phe Thr Ile Ser His Asp Asn Ala Glu Asn 
                85                  90                  95      


Thr Ile Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr 
        115                 120                 125             


Cys Glu Gly Asp Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Ser Met Asp Pro Gly Gly Ser His His His His His His 
145                 150                 155                 160 


His His 
        


<210>  12
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-K64Q-RDNSE-cHis

<400>  12

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val 
            20                  25                  30          


Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser 
        35                  40                  45              


Leu Asp Asp Ser Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val 
    50                  55                  60                  


Cys Gln Leu Val Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr 
65                  70                  75                  80  


Pro Ser Val Gln Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Glu Asn 
                85                  90                  95      


Thr Ile Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr 
        115                 120                 125             


Cys Glu Gly Asp Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
    130                 135                 140                 


Ser Ser Gly Ser Met Asp Pro Gly Gly Ser His His His His His His 
145                 150                 155                 160 


His His 
        


<210>  13
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ͻCDR2

<400>  13

Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Gln Gly 
1               5                   10                  15  


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  һ

<400>  14
gtcctggctg ctcttctaca aggc                                              24


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  һ

<400>  15
ggtacgtgct gttgaactgt tcc                                               23


<210>  16
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ڶ

<400>  16
gatgtgcagc tgcaggagtc tggrggagg                                         29


<210>  17
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ڶ

<400>  17
ggactagtgc ggccgctgga gacggtgacc tgggt                                  35


<210>  18
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-K86R&P87A

<400>  18

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Asp Asp Ser 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val Cys Gln Leu Val 
        35                  40                  45              


Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser His Asp Arg Ala Lys Asn Thr Ile Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr Cys Glu Gly Asp 
            100                 105                 110         


Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  19
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-RDNSE

<400>  19

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Asp Asp Ser 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val Cys Gln Leu Val 
        35                  40                  45              


Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Glu Asn Thr Ile Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr Cys Glu Gly Asp 
            100                 105                 110         


Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  20
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-K64Q

<400>  20

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Asp Asp Ser 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val Cys Gln Leu Val 
        35                  40                  45              


Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Gln 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser His Asp Arg Ala Lys Asn Thr Ile Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr Cys Glu Gly Asp 
            100                 105                 110         


Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  21
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-R73N&K75E

<400>  21

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Asp Asp Ser 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val Cys Gln Leu Val 
        35                  40                  45              


Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser His Asp Asn Ala Glu Asn Thr Ile Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr Cys Glu Gly Asp 
            100                 105                 110         


Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  22
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  109-K64Q-RDNSE

<400>  22

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Ser Leu Asp Asp Ser 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Arg Gly Asn Val Cys Gln Leu Val 
        35                  40                  45              


Ser Thr Ile Ala Ser Asp Arg Ser Thr Tyr Tyr Thr Pro Ser Val Gln 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Glu Asn Thr Ile Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Ala Pro Arg Leu Ala Tyr Thr Thr Ala Met Thr Cys Glu Gly Asp 
            100                 105                 110         


Phe Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


