                         SEQUENCE LISTING

<110>  MEDICAGO INC.
 
<120>  Influenza Virus Hemagglutinin Mutants

<130>  V811304WO

<150>  US 62/690,780
<151>  2018-06-27

<160>  148   

<170>  PatentIn version 3.5

<210>  1
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Cal DNA

<400>  1
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg ctgacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagctcatgg      300

tcctacattg tggaaacacc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcatgctg gagcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcaa atcctacatt aatgataaag ggaaagaagt cctcgtgcta      600

tggggcattc accatccatc tactagtgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggtc atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcgca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc aaacacccaa gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaaatat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atatcccgtc tattcaatct agaggactat ttggggccat tgccggtttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaga atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt aggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttatat gaaaaggtaa gaagccagct aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaagaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagtttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  2
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Cal AA

<400>  2

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Pro Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Lys Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Ser Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Ser Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Ala Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Lys Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Ser Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Glu Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  3
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich DNA

<400>  3
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  4
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich AA

<400>  4

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  5
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass DNA

<400>  5
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  6
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass AA

<400>  6

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  7
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CostaR DNA

<400>  7
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacgtg aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaaagtac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  8
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CostaR AA

<400>  8

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Val Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Lys Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  9
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond DNA

<400>  9
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtacccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaaccac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaaggga gcaattgagc tcagtgtcat catttgagag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac agcagcatgt      480

cctcacgctg gggcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag gaaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttgggaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggcgat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgaccagaag agcacacaaa gtgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctattgg aaaatgaaag aactttggac     1380

taccacgact caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  10
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond AA

<400>  10

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Pro Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Pro Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Gln Lys Ser Thr Gln Ser Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  11
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw DNA

<400>  11
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cacaacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaacccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aatttaatat ggctaactaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag ggaaagaaat cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagcca      780

ggtgacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtgaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag ggtatgcagc cgacctgaag agcacacaaa atgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctattgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaatgg ttgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact caggggaagc aaaattaaac     1560

agagaaaaaa tagaaggggt aaagctggaa tcaacaagaa tttaccaaat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  12
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw AA

<400>  12

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Thr 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Ile Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Gly Glu Ala Lys Leu Asn Arg Glu Lys Ile Glu Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  13
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-CPMV(fl5'UTR)_SpPDI.c

<400>  13
tcgtgcttcg gcaccagtac aatggcgaaa aacgttgcga ttttcggct                   49


<210>  14
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H1cTMCT.S1-4r

<400>  14
actaaagaaa ataggccttt aaatacatat tctacactgt agagac                      46


<210>  15
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Cal(F390D).r

<400>  15
ctctttacct actgctgtgt cctgtgtatt catcttttca ataacagaat tta              53


<210>  16
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Cal(F390D).c

<400>  16
ttattgaaaa gatgaataca caggacacag cagtaggtaa agagttcaac                  50


<210>  17
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Cal-F390D DNA sequence

<400>  17
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagctcatgg      300

tcctacattg tggaaacacc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcatgctg gagcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcaa atcctacatt aatgataaag ggaaagaagt cctcgtgcta      600

tggggcattc accatccatc tactagtgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggtc atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcgca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc aaacacccaa gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaaatat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atatcccgtc tattcaatct agaggactat ttggggccat tgccggtttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaga atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt aggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttatat gaaaaggtaa gaagccagct aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaagaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagtttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  18
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Cal-F390D AA sequence

<400>  18

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Pro Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Lys Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Ser Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Ser Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Ala Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Lys Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Ser Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Glu Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  19
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Cal(L429M).r

<400>  19
gttctttcat tttccattag aaccaacagt tcggcattgt aagtccaa                    48


<210>  20
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Cal(L429M).c

<400>  20
cgaactgttg gttctaatgg aaaatgaaag aactttggac taccacga                    48


<210>  21
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Cal-L429M DNA sequence

<400>  21
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagctcatgg      300

tcctacattg tggaaacacc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcatgctg gagcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcaa atcctacatt aatgataaag ggaaagaagt cctcgtgcta      600

tggggcattc accatccatc tactagtgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggtc atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcgca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc aaacacccaa gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaaatat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atatcccgtc tattcaatct agaggactat ttggggccat tgccggtttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaga atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt aggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttatat gaaaaggtaa gaagccagct aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaagaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagtttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  22
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Cal-L429M AA sequence

<400>  22

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Pro Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Lys Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Ser Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Ser Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Ala Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Lys Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Ser Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Glu Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  23
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Cal-F390D+L429M DNA sequence

<400>  23
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagctcatgg      300

tcctacattg tggaaacacc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcatgctg gagcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcaa atcctacatt aatgataaag ggaaagaagt cctcgtgcta      600

tggggcattc accatccatc tactagtgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggtc atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcgca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc aaacacccaa gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaaatat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atatcccgtc tattcaatct agaggactat ttggggccat tgccggtttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaga atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt aggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttatat gaaaaggtaa gaagccagct aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaagaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagtttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  24
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Cal-F390D+L429M AA sequence

<400>  24

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Pro Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Lys Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Ser Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Ser Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Ala Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Lys Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Ser Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Glu Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  25
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Mich(N97D).r

<400>  25
tagctcctca taatcgatga aatctcctgg gtaacacgtt ccattgtctg aa               52


<210>  26
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1Mich(N97D).c

<400>  26
cccaggagat ttcatcgatt atgaggagct aagagagcaa ttgagctcag                  50


<210>  27
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N97D DNA

<400>  27
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  28
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N97D AA

<400>  28

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  29
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Mich(K374E).r

<400>  29
tttgttagta atctcgtcaa tggcattttg tgtgctcttc aggtcggctg catatcc          57


<210>  30
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Mich(K374E).c

<400>  30
acaaaatgcc attgacgaga ttactaacaa agtaaattct gttattgaaa                  50


<210>  31
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-K374E DNA

<400>  31
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  32
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-K374E AA

<400>  32

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  33
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Mich(F390D).r

<400>  33
cccactgctg tgtcctgtgt attcatcttt tcaataacag aatttactt                   49


<210>  34
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Mich(F390D).c

<400>  34
aaagatgaat acacaggaca cagcagtggg taaagagttc aaccacctg                   49


<210>  35
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-F390D DNA

<400>  35
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  36
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-F390D AA

<400>  36

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  37
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Mich(L429M).c

<400>  37
cgaactgttg gttctaatgg aaaatgaaag aactttggac tatcacgatt caaa             54


<210>  38
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-L429M DNA

<400>  38
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  39
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-L429M AA

<400>  39

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  40
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N97D+K374E DNA

<400>  40
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  41
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N97D+K374E AA

<400>  41

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  42
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-F390D+L429M DNA

<400>  42
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  43
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-F390D+L429M AA

<400>  43

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  44
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N97D+F390D+L429M DNA

<400>  44
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  45
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N97D+F390D+L429M AA

<400>  45

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  46
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-K374E+F390D+L429M DNA

<400>  46
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  47
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-K374E+F390D+L429M AA

<400>  47

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  48
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N97D+K374E+F390D+L429M DNA

<400>  48
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  49
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N97D+K374E+F390D+L429M AA

<400>  49

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  50
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass-F390D+L429M DNA

<400>  50
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  51
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass-F390D+L429M AA

<400>  51

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  52
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass-N97D+F390D+L429M DNA

<400>  52
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  53
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass-N97D+F390D+L429M AA

<400>  53

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  54
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass-K374E+F390D+L429M DNA

<400>  54
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  55
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass-K374E+F390D+L429M AA

<400>  55

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  56
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass-N97D+K374E+F390D+L429M DNA

<400>  56
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  57
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mass-N97D+K374E+F390D+L429M AA

<400>  57

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  58
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CR-F390D+L429M DNA

<400>  58
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacgtg aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaaagtac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  59
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CR-F390D+L429M AA

<400>  59

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Val Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Lys Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  60
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CR-N97D+F390D+L429M DNA

<400>  60
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacgtg aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaaagtac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  61
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CR-N97D+F390D+L429M AA

<400>  61

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Val Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Lys Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  62
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CR-K374E+F390D+L429M DNA

<400>  62
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacgtg aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaaagtac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  63
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CR-K374E+F390D+L429M AA

<400>  63

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Val Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Lys Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  64
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CR-N97D+K374E+F390D+L429M DNA

<400>  64
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacgtg aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccacc cactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaaagtac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  65
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 CR-N97D+K374E+F390D+L429M AA

<400>  65

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Val Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Pro Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Lys Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  66
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Hond(N97D).c

<400>  66
cccaggagat ttcatcgatt atgaggagct aagggagcaa ttgagctcag                  50


<210>  67
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-N97D DNA

<400>  67
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtacccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaaccac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaaggga gcaattgagc tcagtgtcat catttgagag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac agcagcatgt      480

cctcacgctg gggcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag gaaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttgggaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggcgat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgaccagaag agcacacaaa gtgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctattgg aaaatgaaag aactttggac     1380

taccacgact caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  68
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-N97D AA

<400>  68

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Pro Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Pro Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Gln Lys Ser Thr Gln Ser Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  69
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Hond(K374E).r

<400>  69
tttgttagta atctcgtcaa tggcactttg tgtgctcttc tggtcggctg catatcc          57


<210>  70
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Hond(K374E).c

<400>  70
acaaagtgcc attgacgaga ttactaacaa agtaaattct gttattgaaa                  50


<210>  71
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-K374E DNA

<400>  71
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtacccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaaccac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaaggga gcaattgagc tcagtgtcat catttgagag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac agcagcatgt      480

cctcacgctg gggcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag gaaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttgggaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggcgat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgaccagaag agcacacaaa gtgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctattgg aaaatgaaag aactttggac     1380

taccacgact caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  72
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-K374E AA

<400>  72

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Pro Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Pro Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Gln Lys Ser Thr Gln Ser Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  73
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Hond(F390D).r

<400>  73
acccactgct gtgtcctgtg tattcatctt ttcaataaca gaatttactt                  50


<210>  74
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1Hond(F390D).c

<400>  74
aaagatgaat acacaggaca cagcagtggg taaagagttc aaccacttgg                  50


<210>  75
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-F390D DNA

<400>  75
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtacccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaaccac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaaggga gcaattgagc tcagtgtcat catttgagag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac agcagcatgt      480

cctcacgctg gggcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag gaaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttgggaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggcgat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgaccagaag agcacacaaa gtgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctattgg aaaatgaaag aactttggac     1380

taccacgact caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  76
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-F390D AA

<400>  76

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Pro Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Pro Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Gln Lys Ser Thr Gln Ser Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  77
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Hond(L429M).r

<400>  77
tctttcattt tccattagaa ccagcagttc ggcattgtaa gtccaaatgt ccagg            55


<210>  78
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Hond(L429M).c

<400>  78
cgaactgctg gttctaatgg aaaatgaaag aactttggac taccacga                    48


<210>  79
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-L429M DNA

<400>  79
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtacccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaaccac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaaggga gcaattgagc tcagtgtcat catttgagag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac agcagcatgt      480

cctcacgctg gggcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag gaaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttgggaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggcgat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgaccagaag agcacacaaa gtgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctaatgg aaaatgaaag aactttggac     1380

taccacgact caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  80
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-L429M AA

<400>  80

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Pro Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Pro Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Gln Lys Ser Thr Gln Ser Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  81
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-F390D+L429M DNA

<400>  81
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtacccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaaccac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaaggga gcaattgagc tcagtgtcat catttgagag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac agcagcatgt      480

cctcacgctg gggcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag gaaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttgggaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggcgat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgaccagaag agcacacaaa gtgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctaatgg aaaatgaaag aactttggac     1380

taccacgact caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  82
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-F390D+L429M AA

<400>  82

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Pro Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Pro Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Gln Lys Ser Thr Gln Ser Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  83
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-N97D+F390D+L429M DNA

<400>  83
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cataacggga aactatgcaa actaagaggg gtacccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaaccac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaaggga gcaattgagc tcagtgtcat catttgagag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac agcagcatgt      480

cctcacgctg gggcaaaaag cttctacaaa aatttaatat ggctagttaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag gaaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttgggaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggcgat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgaccagaag agcacacaaa gtgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctaatgg aaaatgaaag aactttggac     1380

taccacgact caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  84
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Hond-N97D+F390D+L429M AA

<400>  84

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Pro Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Pro Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Gln Lys Ser Thr Gln Ser Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  85
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-N97D DNA

<400>  85
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cacaacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaacccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aatttaatat ggctaactaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag ggaaagaaat cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagcca      780

ggtgacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtgaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag ggtatgcagc cgacctgaag agcacacaaa atgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctattgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaatgg ttgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact caggggaagc aaaattaaac     1560

agagaaaaaa tagaaggggt aaagctggaa tcaacaagaa tttaccaaat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  86
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-N97D AA

<400>  86

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Thr 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Ile Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Gly Glu Ala Lys Leu Asn Arg Glu Lys Ile Glu Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  87
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Darw(K374E).r

<400>  87
tttgttagta atctcgtcaa tggcattttg tgtgctcttc aggtcggctg catacc           56


<210>  88
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-K374E DNA

<400>  88
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cacaacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaacccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aatttaatat ggctaactaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag ggaaagaaat cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagcca      780

ggtgacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtgaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag ggtatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctattgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaatgg ttgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact caggggaagc aaaattaaac     1560

agagaaaaaa tagaaggggt aaagctggaa tcaacaagaa tttaccaaat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  89
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-K374E AA

<400>  89

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Thr 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Ile Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Gly Glu Ala Lys Leu Asn Arg Glu Lys Ile Glu Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  90
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-F390D DNA

<400>  90
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cacaacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaacccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aatttaatat ggctaactaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag ggaaagaaat cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagcca      780

ggtgacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtgaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag ggtatgcagc cgacctgaag agcacacaaa atgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctattgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaatgg ttgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact caggggaagc aaaattaaac     1560

agagaaaaaa tagaaggggt aaagctggaa tcaacaagaa tttaccaaat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  91
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-F390D AA

<400>  91

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Thr 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Ile Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Gly Glu Ala Lys Leu Asn Arg Glu Lys Ile Glu Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  92
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-L429M DNA

<400>  92
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cacaacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaacccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aatttaatat ggctaactaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag ggaaagaaat cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagcca      780

ggtgacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtgaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag ggtatgcagc cgacctgaag agcacacaaa atgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctaatgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaatgg ttgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact caggggaagc aaaattaaac     1560

agagaaaaaa tagaaggggt aaagctggaa tcaacaagaa tttaccaaat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  93
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-L429M AA

<400>  93

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Thr 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Ile Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Gly Glu Ala Lys Leu Asn Arg Glu Lys Ile Glu Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  94
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-F390D+L429M DNA

<400>  94
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cacaacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaacccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aatttaatat ggctaactaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag ggaaagaaat cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagcca      780

ggtgacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtgaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag ggtatgcagc cgacctgaag agcacacaaa atgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctaatgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaatgg ttgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact caggggaagc aaaattaaac     1560

agagaaaaaa tagaaggggt aaagctggaa tcaacaagaa tttaccaaat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  95
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-F390D+L429M AA

<400>  95

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Thr 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Ile Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Gly Glu Ala Lys Leu Asn Arg Glu Lys Ile Glu Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  96
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-N97D+F390D+L429M DNA

<400>  96
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct agaagacaag      180

cacaacggga aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaacccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc tagttcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag atttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aatttaatat ggctaactaa aaaaggaaat      540

tcatacccaa agctcagcca atcctacatt aatgataaag ggaaagaaat cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaata      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagcca      780

ggtgacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtgaaaagca caaaattgag actggccaca     1020

ggattgagga atgtcccatc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag ggtatgcagc cgacctgaag agcacacaaa atgccattga caaaattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccact tggaaaaaag aatagagaat ttaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgctg gttctaatgg aaaatgaaag aactttggac     1380

taccacgatt caaatgtgaa gaacttgtat gaaaaggtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaatgg ttgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact caggggaagc aaaattaaac     1560

agagaaaaaa tagaaggggt aaagctggaa tcaacaagaa tttaccaaat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  97
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Darw-N97D+F390D+L429M AA

<400>  97

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Ser Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Thr 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Ile Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Ile 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Gly Glu Ala Lys Leu Asn Arg Glu Lys Ile Glu Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  98
<211>  4540
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cloning vector 1190 from left to right T-DNA

<400>  98
tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg       60

gacgttttta atgtactgaa ttaacgccga atcccgggct ggtatattta tatgttgtca      120

aataactcaa aaaccataaa agtttaagtt agcaagtgtg tacattttta cttgaacaaa      180

aatattcacc tactactgtt ataaatcatt attaaacatt agagtaaaga aatatggatg      240

ataagaacaa gagtagtgat attttgacaa caattttgtt gcaacatttg agaaaatttt      300

gttgttctct cttttcattg gtcaaaaaca atagagagag aaaaaggaag agggagaata      360

aaaacataat gtgagtatga gagagaaagt tgtacaaaag ttgtaccaaa atagttgtac      420

aaatatcatt gaggaatttg acaaaagcta cacaaataag ggttaattgc tgtaaataaa      480

taaggatgac gcattagaga gatgtaccat tagagaattt ttggcaagtc attaaaaaga      540

aagaataaat tatttttaaa attaaaagtt gagtcatttg attaaacatg tgattattta      600

atgaattgat gaaagagttg gattaaagtt gtattagtaa ttagaatttg gtgtcaaatt      660

taatttgaca tttgatcttt tcctatatat tgccccatag agtcagttaa ctcattttta      720

tatttcatag atcaaataag agaaataacg gtatattaat ccctccaaaa aaaaaaaacg      780

gtatatttac taaaaaatct aagccacgta ggaggataac aggatccccg taggaggata      840

acatccaatc caaccaatca caacaatcct gatgagataa cccactttaa gcccacgcat      900

ctgtggcaca tctacattat ctaaatcaca cattcttcca cacatctgag ccacacaaaa      960

accaatccac atctttatca cccattctat aaaaaatcac actttgtgag tctacacttt     1020

gattcccttc aaacacatac aaagagaaga gactaattaa ttaattaatc atcttgagag     1080

aaaatggaac gagctataca aggaaacgac gctagggaac aagctaacag tgaacgttgg     1140

gatggaggat caggaggtac cacttctccc ttcaaacttc ctgacgaaag tccgagttgg     1200

actgagtggc ggctacataa cgatgagacg aattcgaatc aagataatcc ccttggtttc     1260

aaggaaagct ggggtttcgg gaaagttgta tttaagagat atctcagata cgacaggacg     1320

gaagcttcac tgcacagagt ccttggatct tggacgggag attcggttaa ctatgcagca     1380

tctcgatttt tcggtttcga ccagatcgga tgtacctata gtattcggtt tcgaggagtt     1440

agtatcaccg tttctggagg gtcgcgaact cttcagcatc tctgtgagat ggcaattcgg     1500

tctaagcaag aactgctaca gcttgcccca atcgaagtgg aaagtaatgt atcaagagga     1560

tgccctgaag gtactcaaac cttcgaaaaa gaaagcgagt aagttaaaat gcttcttcgt     1620

ctcctattta taatatggtt tgttattgtt aattttgttc ttgtagaaga gcttaattaa     1680

tcgttgttgt tatgaaatac tatttgtatg agatgaactg gtgtaatgta attcatttac     1740

ataagtggag tcagaatcag aatgtttcct ccataactaa ctagacatga agacctgccg     1800

cgtacaattg tcttatattt gaacaactaa aattgaacat cttttgccac aactttataa     1860

gtggttaata tagctcaaat atatggtcaa gttcaataga ttaataatgg aaatatcagt     1920

tatcgaaatt cattaacaat caacttaacg ttattaacta ctaattttat atcatcccct     1980

ttgataaatg atagtacacc aattaggaag gagcatgctc gcctaggaga ttgtcgtttc     2040

ccgccttcag tttgcaagct gctctagccg tgtagccaat acgcaaaccg cctctccccg     2100

cgcgttggga attactagcg cgtgtcgaca agcttgcatg ccggtcaaca tggtggagca     2160

cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat     2220

tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat     2280

ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg     2340

cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc     2400

cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt     2460

ggattgatgt gataacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga     2520

tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa     2580

cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga     2640

aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc     2700

tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga     2760

cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga     2820

tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca     2880

tttggagagg tattaaaatc ttaataggtt ttgataaaag cgaacgtggg gaaacccgaa     2940

ccaaaccttc ttctaaactc tctctcatct ctcttaaagc aaacttctct cttgtctttc     3000

ttgcgtgagc gatcttcaac gttgtcagat cgtgcttcgg caccgcggat ggcgaaaaac     3060

gttgcgattt tcggcttatt gttttctctt cttgtgttgg ttccttctca gatcttcgcc     3120

tgcaggctcc tcagccaaaa cgacaccccc atctgtctat ccactggccc ctggatctgc     3180

tgcccaaact aactccatgg tgaccctggg atgcctggtc aagggctatt tccctgagcc     3240

agtgacagtg acctggaact ctggatccct gtccagcggt gtgcacacct tcccagctgt     3300

cctgcagtct gacctctaca ctctgagcag ctcagtgact gtcccctcca gcacctggcc     3360

cagcgagacc gtcacctgca acgttgccca cccggccagc agcaccaagg tggacaagaa     3420

aattgtgccc agggattgtg gttgtaagcc ttgcatatgt acagtcccag aagtatcatc     3480

tgtcttcatc ttccccccaa agcccaagga tgtgctcacc attactctga ctcctaaggt     3540

cacgtgtgtt gtggtagaca tcagcaagga tgatcccgag gtccagttca gctggtttgt     3600

agatgatgtg gaggtgcaca cagctcagac gcaaccccgg gaggagcagt tcaacagcac     3660

tttccgctca gtcagtgaac ttcccatcat gcaccaggac tggctcaatg gcaaggagcg     3720

atcgctcacc atcaccatca ccatcaccat caccattaaa ggcctatttt ctttagtttg     3780

aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg tgctcagagt     3840

gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt cgtcccttca     3900

gcaaggacac aaaaagattt taattttatt aaaaaaaaaa aaaaaaaaga ccgggaattc     3960

gatatcaagc ttatcgacct gcagatcgtt caaacatttg gcaataaagt ttcttaagat     4020

tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc     4080

atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag     4140

tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata     4200

aattatcgcg cgcggtgtca tctatgttac tagatctcta gagtctcaag cttggcgcgc     4260

ccacgtgact agtggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg     4320

ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag     4380

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca     4440

gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca     4500

ggatatattg gcgggtaaac ctaagagaaa agagcgttta                           4540


<210>  99
<211>  3105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 1314 from 2X35S prom to NOS term

<400>  99
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgctg acacattatg tataggttat catgcgaaca attcaacaga     1020

cactgtagac acagtactag aaaagaatgt aacagtaaca cactctgtta accttctaga     1080

agacaagcat aacgggaaac tatgcaaact aagaggggta gccccattgc atttgggtaa     1140

atgtaacatt gctggctgga tcctgggaaa tccagagtgt gaatcactct ccacagcaag     1200

ctcatggtcc tacattgtgg aaacacctag ttcagacaat ggaacgtgtt acccaggaga     1260

tttcatcgat tatgaggagc taagagagca attgagctca gtgtcatcat ttgaaaggtt     1320

tgagatattc cccaagacaa gttcatggcc caatcatgac tcgaacaaag gtgtaacggc     1380

agcatgtcct catgctggag caaaaagctt ctacaaaaat ttaatatggc tagttaaaaa     1440

aggaaattca tacccaaagc tcagcaaatc ctacattaat gataaaggga aagaagtcct     1500

cgtgctatgg ggcattcacc atccatctac tagtgctgac caacaaagtc tctatcagaa     1560

tgcagatgca tatgtttttg tggggtcatc aagatacagc aagaagttca agccggaaat     1620

agcaataaga cccaaagtga gggatcaaga agggagaatg aactattact ggacactagt     1680

agagccggga gacaaaataa cattcgaagc aactggaaat ctagtggtac cgagatatgc     1740

attcgcaatg gaaagaaatg ctggatctgg tattatcatt tcagatacac cagtccacga     1800

ttgcaataca acttgtcaaa cacccaaggg tgctataaac accagcctcc catttcagaa     1860

tatacatccg atcacaattg gaaaatgtcc aaaatatgta aaaagcacaa aattgagact     1920

ggccacagga ttgaggaata tcccgtctat tcaatctaga ggactatttg gggccattgc     1980

cggtttcatt gaaggggggt ggacagggat ggtagatgga tggtacggtt atcaccatca     2040

aaatgagcag gggtcaggat atgcagccga cctgaagagc acacagaatg ccattgacga     2100

gattactaac aaagtaaatt ctgttattga aaagatgaat acacagttca cagcagtagg     2160

taaagagttc aaccacctgg aaaaaagaat agagaattta aataaaaaag ttgatgatgg     2220

tttcctggac atttggactt acaatgccga actgttggtt ctattggaaa atgaaagaac     2280

tttggactac cacgattcaa atgtgaagaa cttatatgaa aaggtaagaa gccagctaaa     2340

aaacaatgcc aaggaaattg gaaacggctg ctttgaattt taccacaaat gcgataacac     2400

gtgcatggaa agtgtcaaaa atgggactta tgactaccca aaatactcag aggaagcaaa     2460

attaaacaga gaagaaatag atggggtaaa gctggaatca acaaggattt accagatttt     2520

ggcgatctat tcaactgtcg ccagttcatt ggtactggta gtctccctgg gggcaatcag     2580

tttctggatg tgctctaatg ggtctctaca gtgtagaata tgtatttaaa ggcctatttt     2640

ctttagtttg aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg     2700

tgctcagagt gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt     2760

cgtcccttca gcaaggacac aaaaagattt taattttatt aaaaaaaaaa aaaaaaaaga     2820

ccgggaattc gatatcaagc ttatcgacct gcagatcgtt caaacatttg gcaataaagt     2880

ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat     2940

tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt     3000

atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca     3060

aactaggata aattatcgcg cgcggtgtca tctatgttac tagat                     3105


<210>  100
<211>  3105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 2980 from 2X35S prom to NOS term

<400>  100
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgcgg acacattatg tataggttat catgcgaaca attcaacaga     1020

cactgtagac acagtactag aaaagaatgt aacagtaaca cactctgtta accttctaga     1080

agacaagcat aacgggaaac tatgcaaact aagaggggta gccccattgc atttgggtaa     1140

atgtaacatt gctggctgga tcctgggaaa tccagagtgt gaatcactct ccacagcaag     1200

ctcatggtcc tacattgtgg aaacacctag ttcagacaat ggaacgtgtt acccaggaga     1260

tttcatcgat tatgaggagc taagagagca attgagctca gtgtcatcat ttgaaaggtt     1320

tgagatattc cccaagacaa gttcatggcc caatcatgac tcgaacaaag gtgtaacggc     1380

agcatgtcct catgctggag caaaaagctt ctacaaaaat ttaatatggc tagttaaaaa     1440

aggaaattca tacccaaagc tcagcaaatc ctacattaat gataaaggga aagaagtcct     1500

cgtgctatgg ggcattcacc atccatctac tagtgctgac caacaaagtc tctatcagaa     1560

tgcagatgca tatgtttttg tggggtcatc aagatacagc aagaagttca agccggaaat     1620

agcaataaga cccaaagtga gggatcaaga agggagaatg aactattact ggacactagt     1680

agagccggga gacaaaataa cattcgaagc aactggaaat ctagtggtac cgagatatgc     1740

attcgcaatg gaaagaaatg ctggatctgg tattatcatt tcagatacac cagtccacga     1800

ttgcaataca acttgtcaaa cacccaaggg tgctataaac accagcctcc catttcagaa     1860

tatacatccg atcacaattg gaaaatgtcc aaaatatgta aaaagcacaa aattgagact     1920

ggccacagga ttgaggaata tcccgtctat tcaatctaga ggactatttg gggccattgc     1980

cggtttcatt gaaggggggt ggacagggat ggtagatgga tggtacggtt atcaccatca     2040

aaatgagcag gggtcaggat atgcagccga cctgaagagc acacagaatg ccattgacga     2100

gattactaac aaagtaaatt ctgttattga aaagatgaat acacaggaca cagcagtagg     2160

taaagagttc aaccacctgg aaaaaagaat agagaattta aataaaaaag ttgatgatgg     2220

tttcctggac atttggactt acaatgccga actgttggtt ctattggaaa atgaaagaac     2280

tttggactac cacgattcaa atgtgaagaa cttatatgaa aaggtaagaa gccagctaaa     2340

aaacaatgcc aaggaaattg gaaacggctg ctttgaattt taccacaaat gcgataacac     2400

gtgcatggaa agtgtcaaaa atgggactta tgactaccca aaatactcag aggaagcaaa     2460

attaaacaga gaagaaatag atggggtaaa gctggaatca acaaggattt accagatttt     2520

ggcgatctat tcaactgtcg ccagttcatt ggtactggta gtctccctgg gggcaatcag     2580

tttctggatg tgctctaatg ggtctctaca gtgtagaata tgtatttaaa ggcctatttt     2640

ctttagtttg aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg     2700

tgctcagagt gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt     2760

cgtcccttca gcaaggacac aaaaagattt taattttatt aaaaaaaaaa aaaaaaaaga     2820

ccgggaattc gatatcaagc ttatcgacct gcagatcgtt caaacatttg gcaataaagt     2880

ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat     2940

tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt     3000

atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca     3060

aactaggata aattatcgcg cgcggtgtca tctatgttac tagat                     3105


<210>  101
<211>  3105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct 2995 from 2X35S prom to NOS term

<400>  101
gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca       60

gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga      120

ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc      300

acgtcttcaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac acttgtctac      360

tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa      420

agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg      480

aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc      540

atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggtat taaaatctta ataggttttg ataaaagcga      780

acgtggggaa acccgaacca aaccttcttc taaactctct ctcatctctc ttaaagcaaa      840

cttctctctt gtctttcttg cgtgagcgat cttcaacgtt gtcagatcgt gcttcggcac      900

cagtacaatg gcgaaaaacg ttgcgatttt cggcttattg ttttctcttc ttgtgttggt      960

tccttctcag atcttcgcgg acacattatg tataggttat catgcgaaca attcaacaga     1020

cactgtagac acagtactag aaaagaatgt aacagtaaca cactctgtta accttctaga     1080

agacaagcat aacgggaaac tatgcaaact aagaggggta gccccattgc atttgggtaa     1140

atgtaacatt gctggctgga tcctgggaaa tccagagtgt gaatcactct ccacagcaag     1200

ctcatggtcc tacattgtgg aaacacctag ttcagacaat ggaacgtgtt acccaggaga     1260

tttcatcgat tatgaggagc taagagagca attgagctca gtgtcatcat ttgaaaggtt     1320

tgagatattc cccaagacaa gttcatggcc caatcatgac tcgaacaaag gtgtaacggc     1380

agcatgtcct catgctggag caaaaagctt ctacaaaaat ttaatatggc tagttaaaaa     1440

aggaaattca tacccaaagc tcagcaaatc ctacattaat gataaaggga aagaagtcct     1500

cgtgctatgg ggcattcacc atccatctac tagtgctgac caacaaagtc tctatcagaa     1560

tgcagatgca tatgtttttg tggggtcatc aagatacagc aagaagttca agccggaaat     1620

agcaataaga cccaaagtga gggatcaaga agggagaatg aactattact ggacactagt     1680

agagccggga gacaaaataa cattcgaagc aactggaaat ctagtggtac cgagatatgc     1740

attcgcaatg gaaagaaatg ctggatctgg tattatcatt tcagatacac cagtccacga     1800

ttgcaataca acttgtcaaa cacccaaggg tgctataaac accagcctcc catttcagaa     1860

tatacatccg atcacaattg gaaaatgtcc aaaatatgta aaaagcacaa aattgagact     1920

ggccacagga ttgaggaata tcccgtctat tcaatctaga ggactatttg gggccattgc     1980

cggtttcatt gaaggggggt ggacagggat ggtagatgga tggtacggtt atcaccatca     2040

aaatgagcag gggtcaggat atgcagccga cctgaagagc acacagaatg ccattgacga     2100

gattactaac aaagtaaatt ctgttattga aaagatgaat acacaggaca cagcagtagg     2160

taaagagttc aaccacctgg aaaaaagaat agagaattta aataaaaaag ttgatgatgg     2220

tttcctggac atttggactt acaatgccga actgttggtt ctaatggaaa atgaaagaac     2280

tttggactac cacgattcaa atgtgaagaa cttatatgaa aaggtaagaa gccagctaaa     2340

aaacaatgcc aaggaaattg gaaacggctg ctttgaattt taccacaaat gcgataacac     2400

gtgcatggaa agtgtcaaaa atgggactta tgactaccca aaatactcag aggaagcaaa     2460

attaaacaga gaagaaatag atggggtaaa gctggaatca acaaggattt accagatttt     2520

ggcgatctat tcaactgtcg ccagttcatt ggtactggta gtctccctgg gggcaatcag     2580

tttctggatg tgctctaatg ggtctctaca gtgtagaata tgtatttaaa ggcctatttt     2640

ctttagtttg aatttactgt tattcggtgt gcatttctat gtttggtgag cggttttctg     2700

tgctcagagt gtgtttattt tatgtaattt aatttctttg tgagctcctg tttagcaggt     2760

cgtcccttca gcaaggacac aaaaagattt taattttatt aaaaaaaaaa aaaaaaaaga     2820

ccgggaattc gatatcaagc ttatcgacct gcagatcgtt caaacatttg gcaataaagt     2880

ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat     2940

tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt     3000

atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca     3060

aactaggata aattatcgcg cgcggtgtca tctatgttac tagat                     3105


<210>  102
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Mich(N380A).r

<400>  102
ttcaataaca gaagctactt tgttagtaat cttgtcaatg gcattttgt                   49


<210>  103
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H1Cal(N380A).c

<400>  103
gattactaac aaagtagctt ctgttattga aaagatgaat acacagtt                    48


<210>  104
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N380A DNA

<400>  104
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtag cttctgttat tgaaaagatg aatacacagt tcacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  105
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-N380A AA

<400>  105

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Ala Ser Val Ile Glu Lys Met Asn Thr Gln Phe Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  106
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1Mich(N380A+F390D).r

<400>  106
cccactgctg tgtcctgtgt attcatcttt tcaataacag aagctactt                   49


<210>  107
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-F390D+N380A DNA

<400>  107
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagttcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatatacat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtag cttctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctattgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  108
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Mich-F390D+N380A AA

<400>  108

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Ser Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Ile His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Ala Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Leu Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  109
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H5ITMCT.s1-4r

<400>  109
actaaagaaa ataggccttt aaatgcaaat tctgcattgt aacgatccat                  50


<210>  110
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Indo DNA

<400>  110
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg ccgatcagat ttgcattggt taccatgcaa acaattcaac agagcaggtt      120

gacacaatca tggaaaagaa cgttactgtt acacatgccc aagacatact ggaaaagaca      180

cacaacggga agctctgcga tctagatgga gtgaagcctc taattttaag agattgtagt      240

gtagctggat ggctcctcgg gaacccaatg tgtgacgaat tcatcaatgt accggaatgg      300

tcttacatag tggagaaggc caatccaacc aatgacctct gttacccagg gagtttcaac      360

gactatgaag aactgaaaca cctattgagc agaataaacc attttgagaa aattcaaatc      420

atccccaaaa gttcttggtc cgatcatgaa gcctcatcag gagttagctc agcatgtcca      480

tacctgggaa gtccctcctt ttttagaaat gtggtatggc ttatcaaaaa gaacagtaca      540

tacccaacaa taaagaaaag ctacaataat accaaccaag aggatctttt ggtactgtgg      600

ggaattcacc atcctaatga tgcggcagag cagacaaggc tatatcaaaa cccaaccacc      660

tatatttcca ttgggacatc aacactaaac cagagattgg taccaaaaat agctactaga      720

tccaaagtaa acgggcaaag tggaaggatg gagttcttct ggacaatttt aaaacctaat      780

gatgcaatca acttcgagag taatggaaat ttcattgctc cagaatatgc atacaaaatt      840

gtcaagaaag gggactcagc aattatgaaa agtgaattgg aatatggtaa ctgcaacacc      900

aagtgtcaaa ctccaatggg ggcgataaac tctagtatgc cattccacaa catacaccct      960

ctcaccatcg gggaatgccc caaatatgtg aaatcaaaca gattagtcct tgcaacaggg     1020

ctcagaaata gccctcaaag agagagcaga agaaaaaaga gaggactatt tggagctata     1080

gcaggtttta tagagggagg atggcaggga atggtagatg gttggtatgg gtaccaccat     1140

agcaatgagc aggggagtgg gtacgctgca gacaaagaat ccactcaaaa ggcaatagat     1200

ggagtcacca ataaggtcaa ctcaatcatt gacaaaatga acactcagtt tgaggccgtt     1260

ggaagggaat ttaataactt agaaaggaga atagagaatt taaacaagaa gatggaagac     1320

gggtttctag atgtctggac ttataatgcc gaacttctgg ttctcatgga aaatgagaga     1380

actctagact ttcatgactc aaatgttaag aacctctacg acaaggtccg actacagctt     1440

agggataatg caaaggagct gggtaacggt tgtttcgagt tctatcacaa atgtgataat     1500

gaatgtatgg aaagtataag aaacggaacg tacaactatc cgcagtattc agaagaagca     1560

agattaaaaa gagaggaaat aagtggggta aaattggaat caataggaac ttaccaaata     1620

ctgtcaattt attcaacagt ggcgagttcc ctagcactgg caatcatgat ggctggtcta     1680

tctttatgga tgtgctccaa tggatcgtta caatgcagaa tttgcattta a              1731


<210>  111
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Indo AA

<400>  111

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Gln Ile Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Thr His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Asp Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser 
65                  70                  75                  80  


Val Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn 
                85                  90                  95      


Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Thr Asn Asp 
            100                 105                 110         


Leu Cys Tyr Pro Gly Ser Phe Asn Asp Tyr Glu Glu Leu Lys His Leu 
        115                 120                 125             


Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser 
    130                 135                 140                 


Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro 
145                 150                 155                 160 


Tyr Leu Gly Ser Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys 
                165                 170                 175     


Lys Asn Ser Thr Tyr Pro Thr Ile Lys Lys Ser Tyr Asn Asn Thr Asn 
            180                 185                 190         


Gln Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro Asn Asp Ala 
        195                 200                 205             


Ala Glu Gln Thr Arg Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Ile 
    210                 215                 220                 


Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg 
225                 230                 235                 240 


Ser Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe Trp Thr Ile 
                245                 250                 255     


Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile 
            260                 265                 270         


Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Ile 
        275                 280                 285             


Met Lys Ser Glu Leu Glu Tyr Gly Asn Cys Asn Thr Lys Cys Gln Thr 
    290                 295                 300                 


Pro Met Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn Ile His Pro 
305                 310                 315                 320 


Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn Arg Leu Val 
                325                 330                 335     


Leu Ala Thr Gly Leu Arg Asn Ser Pro Gln Arg Glu Ser Arg Arg Lys 
            340                 345                 350         


Lys Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
        355                 360                 365             


Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn Glu Gln 
    370                 375                 380                 


Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala Ile Asp 
385                 390                 395                 400 


Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln 
                405                 410                 415     


Phe Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu 
            420                 425                 430         


Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr 
        435                 440                 445             


Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe 
    450                 455                 460                 


His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu 
465                 470                 475                 480 


Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His 
                485                 490                 495     


Lys Cys Asp Asn Glu Cys Met Glu Ser Ile Arg Asn Gly Thr Tyr Asn 
            500                 505                 510         


Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser 
        515                 520                 525             


Gly Val Lys Leu Glu Ser Ile Gly Thr Tyr Gln Ile Leu Ser Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met Met Ala Gly Leu 
545                 550                 555                 560 


Ser Leu Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  112
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H5Ind(F393D).r

<400>  112
cttccaacgg cctcgtcctg agtgttcatt ttgtcaatga ttgagttga                   49


<210>  113
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H5Ind(F393D).c

<400>  113
caaaatgaac actcaggacg aggccgttgg aagggaattt aataactta                   49


<210>  114
<211>  1731
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Indo-F393D DNA

<400>  114
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcagat ttgcattggt taccatgcaa acaattcaac agagcaggtt      120

gacacaatca tggaaaagaa cgttactgtt acacatgccc aagacatact ggaaaagaca      180

cacaacggga agctctgcga tctagatgga gtgaagcctc taattttaag agattgtagt      240

gtagctggat ggctcctcgg gaacccaatg tgtgacgaat tcatcaatgt accggaatgg      300

tcttacatag tggagaaggc caatccaacc aatgacctct gttacccagg gagtttcaac      360

gactatgaag aactgaaaca cctattgagc agaataaacc attttgagaa aattcaaatc      420

atccccaaaa gttcttggtc cgatcatgaa gcctcatcag gagttagctc agcatgtcca      480

tacctgggaa gtccctcctt ttttagaaat gtggtatggc ttatcaaaaa gaacagtaca      540

tacccaacaa taaagaaaag ctacaataat accaaccaag aggatctttt ggtactgtgg      600

ggaattcacc atcctaatga tgcggcagag cagacaaggc tatatcaaaa cccaaccacc      660

tatatttcca ttgggacatc aacactaaac cagagattgg taccaaaaat agctactaga      720

tccaaagtaa acgggcaaag tggaaggatg gagttcttct ggacaatttt aaaacctaat      780

gatgcaatca acttcgagag taatggaaat ttcattgctc cagaatatgc atacaaaatt      840

gtcaagaaag gggactcagc aattatgaaa agtgaattgg aatatggtaa ctgcaacacc      900

aagtgtcaaa ctccaatggg ggcgataaac tctagtatgc cattccacaa catacaccct      960

ctcaccatcg gggaatgccc caaatatgtg aaatcaaaca gattagtcct tgcaacaggg     1020

ctcagaaata gccctcaaag agagagcaga agaaaaaaga gaggactatt tggagctata     1080

gcaggtttta tagagggagg atggcaggga atggtagatg gttggtatgg gtaccaccat     1140

agcaatgagc aggggagtgg gtacgctgca gacaaagaat ccactcaaaa ggcaatagat     1200

ggagtcacca ataaggtcaa ctcaatcatt gacaaaatga acactcagga cgaggccgtt     1260

ggaagggaat ttaataactt agaaaggaga atagagaatt taaacaagaa gatggaagac     1320

gggtttctag atgtctggac ttataatgcc gaacttctgg ttctcatgga aaatgagaga     1380

actctagact ttcatgactc aaatgttaag aacctctacg acaaggtccg actacagctt     1440

agggataatg caaaggagct gggtaacggt tgtttcgagt tctatcacaa atgtgataat     1500

gaatgtatgg aaagtataag aaacggaacg tacaactatc cgcagtattc agaagaagca     1560

agattaaaaa gagaggaaat aagtggggta aaattggaat caataggaac ttaccaaata     1620

ctgtcaattt attcaacagt ggcgagttcc ctagcactgg caatcatgat ggctggtcta     1680

tctttatgga tgtgctccaa tggatcgtta caatgcagaa tttgcattta a              1731


<210>  115
<211>  576
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Indo-F393D AA

<400>  115

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Gln Ile Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Thr His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Asp Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser 
65                  70                  75                  80  


Val Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Ile Asn 
                85                  90                  95      


Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ala Asn Pro Thr Asn Asp 
            100                 105                 110         


Leu Cys Tyr Pro Gly Ser Phe Asn Asp Tyr Glu Glu Leu Lys His Leu 
        115                 120                 125             


Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Ser 
    130                 135                 140                 


Ser Trp Ser Asp His Glu Ala Ser Ser Gly Val Ser Ser Ala Cys Pro 
145                 150                 155                 160 


Tyr Leu Gly Ser Pro Ser Phe Phe Arg Asn Val Val Trp Leu Ile Lys 
                165                 170                 175     


Lys Asn Ser Thr Tyr Pro Thr Ile Lys Lys Ser Tyr Asn Asn Thr Asn 
            180                 185                 190         


Gln Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro Asn Asp Ala 
        195                 200                 205             


Ala Glu Gln Thr Arg Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Ile 
    210                 215                 220                 


Gly Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg 
225                 230                 235                 240 


Ser Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe Trp Thr Ile 
                245                 250                 255     


Leu Lys Pro Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile 
            260                 265                 270         


Ala Pro Glu Tyr Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Ala Ile 
        275                 280                 285             


Met Lys Ser Glu Leu Glu Tyr Gly Asn Cys Asn Thr Lys Cys Gln Thr 
    290                 295                 300                 


Pro Met Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn Ile His Pro 
305                 310                 315                 320 


Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn Arg Leu Val 
                325                 330                 335     


Leu Ala Thr Gly Leu Arg Asn Ser Pro Gln Arg Glu Ser Arg Arg Lys 
            340                 345                 350         


Lys Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp 
        355                 360                 365             


Gln Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn Glu Gln 
    370                 375                 380                 


Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Lys Ala Ile Asp 
385                 390                 395                 400 


Gly Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln 
                405                 410                 415     


Asp Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu 
            420                 425                 430         


Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr 
        435                 440                 445             


Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe 
    450                 455                 460                 


His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu 
465                 470                 475                 480 


Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His 
                485                 490                 495     


Lys Cys Asp Asn Glu Cys Met Glu Ser Ile Arg Asn Gly Thr Tyr Asn 
            500                 505                 510         


Tyr Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser 
        515                 520                 525             


Gly Val Lys Leu Glu Ser Ile Gly Thr Tyr Gln Ile Leu Ser Ile Tyr 
    530                 535                 540                 


Ser Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met Met Ala Gly Leu 
545                 550                 555                 560 


Ser Leu Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575     


<210>  116
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer IF-H5_Egy.r

<400>  116
actaaagaaa ataggccttt aaatgcaaat tctgcattgt agcgatccat t                51


<210>  117
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Egypt DNA

<400>  117
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcagat ttgcattggt taccatgcaa acaactcgac agagcaggtt      120

gacacaataa tggaaaagaa tgtcactgtt acacacgccc aagacatact ggaaaagaca      180

cacaacggga aactctgcaa tctagatgga gtgaagcctc tcattttgag agattgtagt      240

gtagctggat ggctcctcgg gaacccaatg tgcgatgaat tcctcaatgt gccggaatgg      300

tcttacatag tggagaaaat caatccagcc aatgacctct gttatccagg gaatttcaac      360

gactatgaag aactgaaaca cctattgagc agaataaacc attttgagaa aattcagatc      420

attcccaaag attcttggtc agatcatgaa gcctcgggag tgagctcagc atgcccatac      480

caaggaagat cctccttttt tagaaatgtt gtatggctta ccaaaaagaa cgatgcatac      540

ccaacaataa agaaaagtta caataatact aaccaagaag atcttttggt actatggggg      600

attcaccatc caaatgatgc tgcagagcag acaaggcttt atcaaaaccc aactacctat      660

atctccgttg ggacatcaac actaaaccag agattggtac ccaaaatagc tactagatct      720

aaggtaaacg ggcaaagtgg aaggatggag ttcttttgga caattttaaa atcgaatgat      780

gcaataaact ttgagagcaa tggaaacttc attgctccag aaaatgcata caaaattgtc      840

aagaaaggag attcaacaat tatgaaaagt gagttggaat atagtaactg caacaccaag      900

tgtcagactc caataggggc gataaactcc agtatgccat tccacaacat ccaccctctc      960

accatcgggg aatgccccaa atatgtgaaa tcaaacagat tagtccttgc tactgggctc     1020

aggaatagcc ctcaaggaga gaaaagaaga aaaaagagag gactattcgg agccatagca     1080

ggctttatag agggaggatg gcagggaatg gtagatggtt ggtatgggta ccaccatagc     1140

aacgagcagg ggagtgggta cgctgcagac aaagaatcca ctcaaagggc tatagatgga     1200

gtcaccaata aggtcaattc gatcattgac aaaatgaaca ctcagtttga ggctgttgga     1260

agggaattta ataacttaga aaggagaata gaaaatttaa acaagaagat ggaagacgga     1320

ttcctagatg tctggactta taatgctgaa cttctggttc tcatggaaaa tgagagaact     1380

ctagactttc atgactcaaa tgtcaagaat ctttatgaca aggtccgact acagcttagg     1440

gataatgcaa aggagcttgg taacggttgt ttcgagttct atcacagatg tgataatgaa     1500

tgtatggaaa gtgtaagaaa cggaacgtat gactaccctc aatattcaga agaagcaaga     1560

ttaaaaagag aggaaataag tggagtaaaa ttggagtcaa taggaactta ccaaatactg     1620

tcaatttatt caacagtggc gagctcccta gcactggcaa tcatggtggc tggtctatct     1680

ttatggatgt gctccaatgg atcgctacaa tgcagaattt gcatttaa                  1728


<210>  118
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Egypt AA

<400>  118

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Gln Ile Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Thr His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Asn Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser 
65                  70                  75                  80  


Val Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Leu Asn 
                85                  90                  95      


Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ile Asn Pro Ala Asn Asp 
            100                 105                 110         


Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His Leu 
        115                 120                 125             


Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Asp 
    130                 135                 140                 


Ser Trp Ser Asp His Glu Ala Ser Gly Val Ser Ser Ala Cys Pro Tyr 
145                 150                 155                 160 


Gln Gly Arg Ser Ser Phe Phe Arg Asn Val Val Trp Leu Thr Lys Lys 
                165                 170                 175     


Asn Asp Ala Tyr Pro Thr Ile Lys Lys Ser Tyr Asn Asn Thr Asn Gln 
            180                 185                 190         


Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro Asn Asp Ala Ala 
        195                 200                 205             


Glu Gln Thr Arg Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val Gly 
    210                 215                 220                 


Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg Ser 
225                 230                 235                 240 


Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe Trp Thr Ile Leu 
                245                 250                 255     


Lys Ser Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala 
            260                 265                 270         


Pro Glu Asn Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Thr Ile Met 
        275                 280                 285             


Lys Ser Glu Leu Glu Tyr Ser Asn Cys Asn Thr Lys Cys Gln Thr Pro 
    290                 295                 300                 


Ile Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn Ile His Pro Leu 
305                 310                 315                 320 


Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn Arg Leu Val Leu 
                325                 330                 335     


Ala Thr Gly Leu Arg Asn Ser Pro Gln Gly Glu Lys Arg Arg Lys Lys 
            340                 345                 350         


Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Gln 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn Glu Gln Gly 
    370                 375                 380                 


Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Arg Ala Ile Asp Gly 
385                 390                 395                 400 


Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln Phe 
                405                 410                 415     


Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu Asn 
            420                 425                 430         


Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe His 
    450                 455                 460                 


Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu Arg 
465                 470                 475                 480 


Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His Arg 
                485                 490                 495     


Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn Gly Thr Tyr Asp Tyr 
            500                 505                 510         


Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser Gly 
        515                 520                 525             


Val Lys Leu Glu Ser Ile Gly Thr Tyr Gln Ile Leu Ser Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met Val Ala Gly Leu Ser 
545                 550                 555                 560 


Leu Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  119
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H5Egy(F392D).r

<400>  119
cttccaacag cctcgtcctg agtgttcatt ttgtcaatga tcgaattga                   49


<210>  120
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer H5Egy(F392D).c

<400>  120
caaaatgaac actcaggacg aggctgttgg aagggaattt aataactta                   49


<210>  121
<211>  1728
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Egypt-F392D DNA

<400>  121
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggatcagat ttgcattggt taccatgcaa acaactcgac agagcaggtt      120

gacacaataa tggaaaagaa tgtcactgtt acacacgccc aagacatact ggaaaagaca      180

cacaacggga aactctgcaa tctagatgga gtgaagcctc tcattttgag agattgtagt      240

gtagctggat ggctcctcgg gaacccaatg tgcgatgaat tcctcaatgt gccggaatgg      300

tcttacatag tggagaaaat caatccagcc aatgacctct gttatccagg gaatttcaac      360

gactatgaag aactgaaaca cctattgagc agaataaacc attttgagaa aattcagatc      420

attcccaaag attcttggtc agatcatgaa gcctcgggag tgagctcagc atgcccatac      480

caaggaagat cctccttttt tagaaatgtt gtatggctta ccaaaaagaa cgatgcatac      540

ccaacaataa agaaaagtta caataatact aaccaagaag atcttttggt actatggggg      600

attcaccatc caaatgatgc tgcagagcag acaaggcttt atcaaaaccc aactacctat      660

atctccgttg ggacatcaac actaaaccag agattggtac ccaaaatagc tactagatct      720

aaggtaaacg ggcaaagtgg aaggatggag ttcttttgga caattttaaa atcgaatgat      780

gcaataaact ttgagagcaa tggaaacttc attgctccag aaaatgcata caaaattgtc      840

aagaaaggag attcaacaat tatgaaaagt gagttggaat atagtaactg caacaccaag      900

tgtcagactc caataggggc gataaactcc agtatgccat tccacaacat ccaccctctc      960

accatcgggg aatgccccaa atatgtgaaa tcaaacagat tagtccttgc tactgggctc     1020

aggaatagcc ctcaaggaga gaaaagaaga aaaaagagag gactattcgg agccatagca     1080

ggctttatag agggaggatg gcagggaatg gtagatggtt ggtatgggta ccaccatagc     1140

aacgagcagg ggagtgggta cgctgcagac aaagaatcca ctcaaagggc tatagatgga     1200

gtcaccaata aggtcaattc gatcattgac aaaatgaaca ctcaggacga ggctgttgga     1260

agggaattta ataacttaga aaggagaata gaaaatttaa acaagaagat ggaagacgga     1320

ttcctagatg tctggactta taatgctgaa cttctggttc tcatggaaaa tgagagaact     1380

ctagactttc atgactcaaa tgtcaagaat ctttatgaca aggtccgact acagcttagg     1440

gataatgcaa aggagcttgg taacggttgt ttcgagttct atcacagatg tgataatgaa     1500

tgtatggaaa gtgtaagaaa cggaacgtat gactaccctc aatattcaga agaagcaaga     1560

ttaaaaagag aggaaataag tggagtaaaa ttggagtcaa taggaactta ccaaatactg     1620

tcaatttatt caacagtggc gagctcccta gcactggcaa tcatggtggc tggtctatct     1680

ttatggatgt gctccaatgg atcgctacaa tgcagaattt gcatttaa                  1728


<210>  122
<211>  575
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H5 Egypt-F392D AA

<400>  122

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Gln Ile Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Glu Gln Val Asp Thr Ile Met Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ala Gln Asp Ile Leu Glu Lys Thr His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Asn Leu Asp Gly Val Lys Pro Leu Ile Leu Arg Asp Cys Ser 
65                  70                  75                  80  


Val Ala Gly Trp Leu Leu Gly Asn Pro Met Cys Asp Glu Phe Leu Asn 
                85                  90                  95      


Val Pro Glu Trp Ser Tyr Ile Val Glu Lys Ile Asn Pro Ala Asn Asp 
            100                 105                 110         


Leu Cys Tyr Pro Gly Asn Phe Asn Asp Tyr Glu Glu Leu Lys His Leu 
        115                 120                 125             


Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln Ile Ile Pro Lys Asp 
    130                 135                 140                 


Ser Trp Ser Asp His Glu Ala Ser Gly Val Ser Ser Ala Cys Pro Tyr 
145                 150                 155                 160 


Gln Gly Arg Ser Ser Phe Phe Arg Asn Val Val Trp Leu Thr Lys Lys 
                165                 170                 175     


Asn Asp Ala Tyr Pro Thr Ile Lys Lys Ser Tyr Asn Asn Thr Asn Gln 
            180                 185                 190         


Glu Asp Leu Leu Val Leu Trp Gly Ile His His Pro Asn Asp Ala Ala 
        195                 200                 205             


Glu Gln Thr Arg Leu Tyr Gln Asn Pro Thr Thr Tyr Ile Ser Val Gly 
    210                 215                 220                 


Thr Ser Thr Leu Asn Gln Arg Leu Val Pro Lys Ile Ala Thr Arg Ser 
225                 230                 235                 240 


Lys Val Asn Gly Gln Ser Gly Arg Met Glu Phe Phe Trp Thr Ile Leu 
                245                 250                 255     


Lys Ser Asn Asp Ala Ile Asn Phe Glu Ser Asn Gly Asn Phe Ile Ala 
            260                 265                 270         


Pro Glu Asn Ala Tyr Lys Ile Val Lys Lys Gly Asp Ser Thr Ile Met 
        275                 280                 285             


Lys Ser Glu Leu Glu Tyr Ser Asn Cys Asn Thr Lys Cys Gln Thr Pro 
    290                 295                 300                 


Ile Gly Ala Ile Asn Ser Ser Met Pro Phe His Asn Ile His Pro Leu 
305                 310                 315                 320 


Thr Ile Gly Glu Cys Pro Lys Tyr Val Lys Ser Asn Arg Leu Val Leu 
                325                 330                 335     


Ala Thr Gly Leu Arg Asn Ser Pro Gln Gly Glu Lys Arg Arg Lys Lys 
            340                 345                 350         


Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Gln 
        355                 360                 365             


Gly Met Val Asp Gly Trp Tyr Gly Tyr His His Ser Asn Glu Gln Gly 
    370                 375                 380                 


Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr Gln Arg Ala Ile Asp Gly 
385                 390                 395                 400 


Val Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met Asn Thr Gln Asp 
                405                 410                 415     


Glu Ala Val Gly Arg Glu Phe Asn Asn Leu Glu Arg Arg Ile Glu Asn 
            420                 425                 430         


Leu Asn Lys Lys Met Glu Asp Gly Phe Leu Asp Val Trp Thr Tyr Asn 
        435                 440                 445             


Ala Glu Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Phe His 
    450                 455                 460                 


Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys Val Arg Leu Gln Leu Arg 
465                 470                 475                 480 


Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys Phe Glu Phe Tyr His Arg 
                485                 490                 495     


Cys Asp Asn Glu Cys Met Glu Ser Val Arg Asn Gly Thr Tyr Asp Tyr 
            500                 505                 510         


Pro Gln Tyr Ser Glu Glu Ala Arg Leu Lys Arg Glu Glu Ile Ser Gly 
        515                 520                 525             


Val Lys Leu Glu Ser Ile Gly Thr Tyr Gln Ile Leu Ser Ile Tyr Ser 
    530                 535                 540                 


Thr Val Ala Ser Ser Leu Ala Leu Ala Ile Met Val Ala Gly Leu Ser 
545                 550                 555                 560 


Leu Trp Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570                 575 


<210>  123
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Par-F390D+L429M (nt)

<400>  123
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca aacctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  124
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Par-F390D+L429M (aa)

<400>  124

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Thr Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  125
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Par-K374E+F390D+L429M (nt)

<400>  125
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca aacctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  126
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Par-K374E+F390D+L429M (aa)

<400>  126

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Thr Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  127
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Par-N97D+F390D+L429M (nt)

<400>  127
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca aacctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  128
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Par-N97D+F390D+L429M (aa)

<400>  128

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Thr Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  129
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Par-N97D+K374E+F390D+L429M (nt)

<400>  129
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca aacctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca cgattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  130
<211>  549
<212>  PRT
<213>  Alphainfluenza A virus

<400>  130

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Ala 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Pro Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Ser Lys Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Ser Ser Arg Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Ala 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser Thr 
        355                 360                 365             


Gln Asn Ala Ile Asp Glu Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  131
<211>  549
<212>  PRT
<213>  Alphainfluenza A virus

<400>  131

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Pro 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Pro Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Ser Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Ser Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Thr 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr 
        355                 360                 365             


Gln Ser Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  132
<211>  549
<212>  PRT
<213>  Alphainfluenza A virus

<400>  132

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Ala 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Ser Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Thr Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Ser Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Ile Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Thr 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser Thr 
        355                 360                 365             


Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Gly Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Lys Ile Glu Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  133
<211>  549
<212>  PRT
<213>  Alphainfluenza A virus

<400>  133

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Ala 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Asn Gln Ser Tyr Val Asn Asp Lys Gly Lys Glu Val Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Pro Thr Thr Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Lys Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Thr 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser Thr 
        355                 360                 365             


Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  134
<211>  549
<212>  PRT
<213>  Alphainfluenza A virus

<400>  134

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Ala 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Thr 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser Thr 
        355                 360                 365             


Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  135
<211>  549
<212>  PRT
<213>  Alphainfluenza A virus

<400>  135

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Ala 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Ser Leu Ser Thr Ala Arg Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Thr 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Val His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser Thr 
        355                 360                 365             


Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  136
<211>  1701
<212>  DNA
<213>  Alphainfluenza A virus

<400>  136
atgaaggcaa tactagtagt tctgctatat acatttacaa ccgcaaatgc agacacatta       60

tgtataggtt atcatgcgaa caattcaaca gacactgtag acacagtact agaaaagaat      120

gtaacagtaa cacactctgt taaccttctg gaagacaagc ataacggaaa actatgcaaa      180

ctaagagggg tagccccatt gcatttgggt aaatgtaaca ttgctggctg gatcctggga      240

aatccagagt gtgaatcact ctccacagca agttcatggt cctacattgt ggaaacatct      300

aattcagaca atggaacgtg ttacccagga gatttcatca attatgagga gctaagagag      360

caattgagct cagtgtcatc atttgaaagg tttgagatat tccccaagac aagttcatgg      420

cccaatcatg actcgaacaa aggtgtaacg gcagcatgtc ctcacgctgg agcaaaaagc      480

ttctacaaaa acttgatatg gctagttaaa aaaggaaatt catacccaaa gcttaaccaa      540

tcctacatta atgataaagg gaaagaagtc ctcgtgctgt ggggcattca ccatccatct      600

actactgctg accaacaaag tctctatcag aatgcagatg catatgtttt tgtggggaca      660

tcaagataca gcaagaagtt caagccggaa atagcaacaa gacccaaagt gagggatcaa      720

gaagggagaa tgaactatta ctggacacta gtagagccgg gagacaaaat aacattcgaa      780

gcaactggaa atctagtggt accgagatat gcattcacaa tggaaagaaa tgctggatct      840

ggtattatca tttcagatac accagtccac gattgcaata caacttgtca gacacccgag      900

ggtgctataa acaccagcct cccatttcag aatatacatc cgatcacaat tggaaaatgt      960

ccaaagtatg taaaaagcac aaaattgaga ctggccacag gattgaggaa tgttccgtct     1020

attcaatcta gaggcctatt cggggccatt gccggcttca ttgaaggggg gtggacaggg     1080

atggtagatg gatggtacgg ttatcaccat caaaatgagc aggggtcagg atatgcagcc     1140

gacctgaaga gcacacaaaa tgccattgac aagattacta acaaagtaaa ttctgttatt     1200

gaaaagatga atacacagtt cacagcagtg ggtaaagagt tcaaccacct ggaaaaaaga     1260

atagagaatc taaataaaaa agttgatgat ggtttcctgg acatttggac ttacaatgcc     1320

gaactgttgg ttctattgga aaatgaaaga actttggact atcacgattc aaatgtgaag     1380

aacttgtatg aaaaagtaag aaaccagtta aaaaacaatg ccaaggaaat tggaaacggc     1440

tgctttgaat tttaccacaa atgcgataac acgtgcatgg aaagtgtcaa aaatgggact     1500

tatgactacc caaaatactc agaggaagca aaattaaaca gagaaaaaat agatggggta     1560

aagctggaat caacaaggat ttaccagatt ttggcgatct attcaactgt cgccagttca     1620

ttggtactgg tagtctccct gggggcaatc agcttctgga tgtgctctaa tgggtctcta     1680

cagtgtagaa tatgtattta a                                               1701


<210>  137
<211>  1701
<212>  DNA
<213>  Alphainfluenza A virus

<400>  137
atgaaggcaa tactagtagt tctgctatat acatttgcaa ccgcaaatgc agacacatta       60

tgtataggtt atcatgcgaa caattcaaca gacactgtag acacagtact agaaaagaat      120

gtaacagtaa cacactctgt taaccttcta gaagacaagc ataacgggaa actatgcaaa      180

ctaagagggg tagccccatt gcatttgggt aaatgtaaca ttgctggctg gatcctggga      240

aatccagagt gtgaatcact ctccacagca agctcatggt cctacattgt ggaaacacct      300

agttcagaca atggaacgtg ttacccagga gatttcatcg attatgagga gctaagagag      360

caattgagct cagtgtcatc atttgaaagg tttgagatat tccccaagac aagttcatgg      420

cccaatcatg actcgaacaa aggtgtaacg gcagcatgtc ctcatgctgg agcaaaaagc      480

ttctacaaaa atttaatatg gctagttaaa aaaggaaatt catacccaaa gctcagcaaa      540

tcctacatta atgataaagg gaaagaagtc ctcgtgctat ggggcattca ccatccatct      600

actagtgctg accaacaaag tctctatcag aatgcagatg catatgtttt tgtggggtca      660

tcaagataca gcaagaagtt caagccggaa atagcaataa gacccaaagt gagggrtcra      720

gaagggagaa tgaactatta ctggacacta gtagagccgg gagacaaaat aacattcgaa      780

gcaactggaa atctagtggt accgagatat gcattcgcaa tggaaagaaa tgctggatct      840

ggtattatca tttcagatac accagtccac gattgcaata caacttgtca aacacccaag      900

ggtgctataa acaccagcct cccatttcag aatatacatc cgatcacaat tggaaaatgt      960

ccaaaatatg taaaaagcac aaaattgaga ctggccacag gattgaggaa tatcccgtct     1020

attcaatcta gaggcctatt tggggccatt gccggtttca ttgaaggggg gtggacaggg     1080

atggtagatg gatggtacgg ttatcaccat caaaatgagc aggggtcagg atatgcagcc     1140

gacctgaaga gcacacagaa tgccattgac gagattacta acaaagtaaa ttctgttatt     1200

gaaaagatga atacacagtt cacagcagta ggtaaagagt tcaaccacct ggaaaaaaga     1260

atagagaatt taaataaaaa agttgatgat ggtttcctgg acatttggac ttacaatgcc     1320

gaactgttgg ttctattgga aaatgaaaga actttggact accacgattc aaatgtgaag     1380

aacttatatg aaaaggtaag aagccagcta aaaaacaatg ccaaggaaat tggaaacggc     1440

tgctttgaat tttaccacaa atgcgataac acgtgcatgg aaagtgtcaa aaatgggact     1500

tatgactacc caaaatactc agaggaagca aaattaaaca gagaagaaat agatggggta     1560

aagctggaat caacaaggat ttaccagatt ttggcgatct attcaactgt cgccagttca     1620

ttggtactgg tagtctccct gggggcaatc agtttctgga tgtgctctaa tgggtctcta     1680

cagtgtagaa tatgtattta a                                               1701


<210>  138
<211>  549
<212>  PRT
<213>  Alphainfluenza A virus

<400>  138

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Ala 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Ser Leu Ser Thr Ala Arg Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Asn Gln Thr Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Thr 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


His Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Val His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser Thr 
        355                 360                 365             


Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  139
<211>  549
<212>  PRT
<213>  Alphainfluenza A virus

<400>  139

Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val 
1               5                   10                  15      


Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn Leu 
            20                  25                  30          


Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val Ala 
        35                  40                  45              


Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly Asn 
    50                  55                  60                  


Pro Glu Cys Glu Ser Leu Ser Thr Ala Arg Ser Trp Ser Tyr Ile Val 
65                  70                  75                  80  


Glu Thr Ser Asn Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe Ile 
                85                  90                  95      


Asn Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe Glu 
            100                 105                 110         


Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp Ser 
        115                 120                 125             


Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser Phe 
    130                 135                 140                 


Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro Lys 
145                 150                 155                 160 


Leu Asn Gln Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val Leu 
                165                 170                 175     


Trp Gly Ile His His Pro Ser Thr Thr Ala Asp Gln Gln Ser Leu Tyr 
            180                 185                 190         


Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser Lys 
        195                 200                 205             


Lys Phe Lys Pro Glu Ile Ala Thr Arg Pro Lys Val Arg Asp Gln Glu 
    210                 215                 220                 


Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys Ile 
225                 230                 235                 240 


Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe Thr 
                245                 250                 255     


Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro Val 
            260                 265                 270         


Gln Asp Cys Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn Thr 
        275                 280                 285             


Ser Leu Pro Phe Gln Asn Val His Pro Ile Thr Ile Gly Lys Cys Pro 
    290                 295                 300                 


Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn 
305                 310                 315                 320 


Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
                325                 330                 335     


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
            340                 345                 350         


His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser Thr 
        355                 360                 365             


Gln Asn Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Ser Val Ile Glu 
    370                 375                 380                 


Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His Leu 
385                 390                 395                 400 


Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu 
                405                 410                 415     


Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu 
            420                 425                 430         


Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu Lys 
        435                 440                 445             


Val Arg Asn Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly Cys 
    450                 455                 460                 


Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val Lys 
465                 470                 475                 480 


Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn 
                485                 490                 495     


Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr Gln 
            500                 505                 510         


Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val 
        515                 520                 525             


Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln 
    530                 535                 540                 


Cys Arg Ile Cys Ile 
545                 


<210>  140
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Par-N97D+K374E+F390D+L429M (aa)

<400>  140

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Thr Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val His Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  141
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Nor-F390D+L429M (nt)

<400>  141
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca ggattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  142
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Nor-F390D+L429M (aa)

<400>  142

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val Gln Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  143
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Nor-K374E+F390D+L429M (nt)

<400>  143
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

aattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca ggattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  144
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Nor-K374E+F390D+L429M (aa)

<400>  144

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asn Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val Gln Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  145
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Nor-N97D+F390D+L429M (nt)

<400>  145
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca ggattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga caagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  146
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Nor-N97D+F390D+L429M (aa)

<400>  146

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val Gln Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Lys Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


<210>  147
<211>  1722
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Nor-N97D+K374E+F390D+L429M (nt)

<400>  147
atggcgaaaa acgttgcgat tttcggctta ttgttttctc ttcttgtgtt ggttccttct       60

cagatcttcg cggacacatt atgtataggt tatcatgcga acaattcaac agacactgta      120

gacacagtac tagaaaagaa tgtaacagta acacactctg ttaaccttct ggaagacaag      180

cataacggaa aactatgcaa actaagaggg gtagccccat tgcatttggg taaatgtaac      240

attgctggct ggatcctggg aaatccagag tgtgaatcac tctccacagc aagatcatgg      300

tcctacattg tggaaacatc taattcagac aatggaacgt gttacccagg agatttcatc      360

gattatgagg agctaagaga gcaattgagc tcagtgtcat catttgaaag gtttgagata      420

ttccccaaga caagttcatg gcccaatcat gactcgaaca aaggtgtaac ggcagcatgt      480

cctcacgctg gagcaaaaag cttctacaaa aacttgatat ggctagttaa aaaaggaaat      540

tcatacccaa agcttaacca atcctacatt aatgataaag ggaaagaagt cctcgtgctg      600

tggggcattc accatccatc tactactgct gaccaacaaa gtctctatca gaatgcagat      660

gcatatgttt ttgtggggac atcaagatac agcaagaagt tcaagccgga aatagcaaca      720

agacccaaag tgagggatca agaagggaga atgaactatt actggacact agtagagccg      780

ggagacaaaa taacattcga agcaactgga aatctagtgg taccgagata tgcattcaca      840

atggaaagaa atgctggatc tggtattatc atttcagata caccagtcca ggattgcaat      900

acaacttgtc agacacccga gggtgctata aacaccagcc tcccatttca gaatgtgcat      960

ccgatcacaa ttggaaaatg tccaaagtat gtaaaaagca caaaattgag actggccaca     1020

ggattgagga atgttccgtc tattcaatct agaggcctat tcggggccat tgccggcttc     1080

attgaagggg ggtggacagg gatggtagat ggatggtacg gttatcacca tcaaaatgag     1140

caggggtcag gatatgcagc cgacctgaag agcacacaaa atgccattga cgagattact     1200

aacaaagtaa attctgttat tgaaaagatg aatacacagg acacagcagt gggtaaagag     1260

ttcaaccacc tggaaaaaag aatagagaat ctaaataaaa aagttgatga tggtttcctg     1320

gacatttgga cttacaatgc cgaactgttg gttctaatgg aaaatgaaag aactttggac     1380

tatcacgatt caaatgtgaa gaacttgtat gaaaaagtaa gaaaccagtt aaaaaacaat     1440

gccaaggaaa ttggaaacgg ctgctttgaa ttttaccaca aatgcgataa cacgtgcatg     1500

gaaagtgtca aaaatgggac ttatgactac ccaaaatact cagaggaagc aaaattaaac     1560

agagaaaaaa tagatggggt aaagctggaa tcaacaagga tttaccagat tttggcgatc     1620

tattcaactg tcgccagttc attggtactg gtagtctccc tgggggcaat cagcttctgg     1680

atgtgctcta atgggtctct acagtgtaga atatgtattt aa                        1722


<210>  148
<211>  573
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PDI-H1 Nor-N97D+K374E+F390D+L429M (aa)

<400>  148

Met Ala Lys Asn Val Ala Ile Phe Gly Leu Leu Phe Ser Leu Leu Val 
1               5                   10                  15      


Leu Val Pro Ser Gln Ile Phe Ala Asp Thr Leu Cys Ile Gly Tyr His 
            20                  25                  30          


Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Val Leu Glu Lys Asn Val 
        35                  40                  45              


Thr Val Thr His Ser Val Asn Leu Leu Glu Asp Lys His Asn Gly Lys 
    50                  55                  60                  


Leu Cys Lys Leu Arg Gly Val Ala Pro Leu His Leu Gly Lys Cys Asn 
65                  70                  75                  80  


Ile Ala Gly Trp Ile Leu Gly Asn Pro Glu Cys Glu Ser Leu Ser Thr 
                85                  90                  95      


Ala Arg Ser Trp Ser Tyr Ile Val Glu Thr Ser Asn Ser Asp Asn Gly 
            100                 105                 110         


Thr Cys Tyr Pro Gly Asp Phe Ile Asp Tyr Glu Glu Leu Arg Glu Gln 
        115                 120                 125             


Leu Ser Ser Val Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Thr 
    130                 135                 140                 


Ser Ser Trp Pro Asn His Asp Ser Asn Lys Gly Val Thr Ala Ala Cys 
145                 150                 155                 160 


Pro His Ala Gly Ala Lys Ser Phe Tyr Lys Asn Leu Ile Trp Leu Val 
                165                 170                 175     


Lys Lys Gly Asn Ser Tyr Pro Lys Leu Asn Gln Ser Tyr Ile Asn Asp 
            180                 185                 190         


Lys Gly Lys Glu Val Leu Val Leu Trp Gly Ile His His Pro Ser Thr 
        195                 200                 205             


Thr Ala Asp Gln Gln Ser Leu Tyr Gln Asn Ala Asp Ala Tyr Val Phe 
    210                 215                 220                 


Val Gly Thr Ser Arg Tyr Ser Lys Lys Phe Lys Pro Glu Ile Ala Thr 
225                 230                 235                 240 


Arg Pro Lys Val Arg Asp Gln Glu Gly Arg Met Asn Tyr Tyr Trp Thr 
                245                 250                 255     


Leu Val Glu Pro Gly Asp Lys Ile Thr Phe Glu Ala Thr Gly Asn Leu 
            260                 265                 270         


Val Val Pro Arg Tyr Ala Phe Thr Met Glu Arg Asn Ala Gly Ser Gly 
        275                 280                 285             


Ile Ile Ile Ser Asp Thr Pro Val Gln Asp Cys Asn Thr Thr Cys Gln 
    290                 295                 300                 


Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro Phe Gln Asn Val His 
305                 310                 315                 320 


Pro Ile Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Lys Leu 
                325                 330                 335     


Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Ser Ile Gln Ser Arg Gly 
            340                 345                 350         


Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly Trp Thr Gly Met 
        355                 360                 365             


Val Asp Gly Trp Tyr Gly Tyr His His Gln Asn Glu Gln Gly Ser Gly 
    370                 375                 380                 


Tyr Ala Ala Asp Leu Lys Ser Thr Gln Asn Ala Ile Asp Glu Ile Thr 
385                 390                 395                 400 


Asn Lys Val Asn Ser Val Ile Glu Lys Met Asn Thr Gln Asp Thr Ala 
                405                 410                 415     


Val Gly Lys Glu Phe Asn His Leu Glu Lys Arg Ile Glu Asn Leu Asn 
            420                 425                 430         


Lys Lys Val Asp Asp Gly Phe Leu Asp Ile Trp Thr Tyr Asn Ala Glu 
        435                 440                 445             


Leu Leu Val Leu Met Glu Asn Glu Arg Thr Leu Asp Tyr His Asp Ser 
    450                 455                 460                 


Asn Val Lys Asn Leu Tyr Glu Lys Val Arg Asn Gln Leu Lys Asn Asn 
465                 470                 475                 480 


Ala Lys Glu Ile Gly Asn Gly Cys Phe Glu Phe Tyr His Lys Cys Asp 
                485                 490                 495     


Asn Thr Cys Met Glu Ser Val Lys Asn Gly Thr Tyr Asp Tyr Pro Lys 
            500                 505                 510         


Tyr Ser Glu Glu Ala Lys Leu Asn Arg Glu Lys Ile Asp Gly Val Lys 
        515                 520                 525             


Leu Glu Ser Thr Arg Ile Tyr Gln Ile Leu Ala Ile Tyr Ser Thr Val 
    530                 535                 540                 


Ala Ser Ser Leu Val Leu Val Val Ser Leu Gly Ala Ile Ser Phe Trp 
545                 550                 555                 560 


Met Cys Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
                565                 570             


