                         SEQUENCE LISTING

<110>  Duke University Office of Licensing and Ventures
 
<120>  COMPOSITIONS AND METHODS FOR THE PRODUCTION OF PYRUVIC ACID AND 
       RELATED PRODUCTS USING DYNAMIC METABOLIC CONTROL

<130>  47381-25

<150>  US 62/687,874
<151>  2018-06-21

<160>  103   

<170>  PatentIn version 3.5

<210>  1
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:  ilcR_tetA_F

<400>  1
taacaataaa aatgaaaatg atttccacga tacagaaaaa agagactgtc atcctaattt       60

ttgttgacac tctatc                                                       76


<210>  2
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:  ilcR_sacB_R

<400>  2
tgccactcag gtatgatggg cagaatattg cctctgcccg ccagaaaaag atcaaaggga       60

aaactgtcca tatgc                                                        75


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: iclR_500up

<400>  3
ccgacaggga ttccatctg                                                    19


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:  iclR_500dn

<400>  4
tatgacgacc attttgtcta cagttc                                            26


<210>  5
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: arcA_tetA_F

<400>  5
ggacttttgt acttcctgtt tcgatttagt tggcaattta ggtagcaaac tcctaatttt       60

tgttgacact ctatc                                                        75


<210>  6
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:  arcA_sacB_R

<400>  6
ataaaaacgg cgctaaaaag cgccgttttt tttgacggtg gtaaagccga atcaaaggga       60

aaactgtcca tatgc                                                        75


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:  arcA_500up

<400>  7
cctgactgta ctaacggttg ag                                                22


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:  arcA_500dn

<400>  8
tgacttttat ggcgttcttt gtttttg                                           27


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:  sspB_kan_F

<400>  9
ctggtacacg ctgatgaaca cc                                                22


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:  sspB_kan_R

<400>  10
ctggtcattg ccatttgtgc c                                                 21


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: sspB_conf_F

<400>  11
gaatcagagc gttccgaccc                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:sspB_conf_R

<400>  12
gtacgcagtt tgccaacgtg                                                   20


<210>  13
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide:cas3_tetA_F

<400>  13
aatagcccgc tgatatcatc gataatacta aaaaaacagg gaggctatta tcctaatttt       60

tgttgacact ctatc                                                        75


<210>  14
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: cas3_sacB_R

<400>  14
tacagggatc cagttatcaa taagcaaatt catttgttct ccttcatatg atcaaaggga       60

aaactgtcca tatgc                                                        75


<210>  15
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: cas3_conf_F

<400>  15
caagacatgt gtatatcact gtaattc                                           27


<210>  16
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: cas3_500dn

<400>  16
gcgattgcag atttatgatt tgg                                               23


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: gltA_conf_F

<400>  17
tatcatcctg aaagcgatgg                                                   20


<210>  18
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: lpd_conf_F

<400>  18
atctcaccgt gtgatcgg                                                     18


<210>  19
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: udhA_conf_F

<400>  19
caaaagagat tctgggtatt cact                                              24


<210>  20
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: zwf_conf_F

<400>  20
ctgctggaaa ccatgcg                                                      17


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: zwf_500dn

<400>  21
agagcatgtc gttataggag gtgat                                             25


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: ampR_intR

<400>  22
agtactcaac caagtcattc tg                                                22


<210>  23
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: bsdR_intR

<400>  23
gagcatggtg atcttctcag t                                                 21


<210>  24
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: gentR_intR

<400>  24
gcgatgaatg tcttactacg ga                                                22


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: purR_intR

<400>  25
gtcgctgggt aatctgcaa                                                    19


<210>  26
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: tetA_intR

<400>  26
atcaacgcat atagcgctag cag                                               23


<210>  27
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: zeoR_intR

<400>  27
actgaagccc agacgatc                                                     18


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: tetR_intR

<400>  28
atcaacgcat atagcgctag cag                                               23


<210>  29
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: specR_intR

<400>  29
cactgtgtgg cttcaggc                                                     18


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: ampR_intR

<400>  30
agtactcaac caagtcattc tg                                                22


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: purR_intR

<400>  31
gtcgctgggt aatctgcaa                                                    19


<210>  32
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: PykA-FOR1

<400>  32
cctgactgct ctctatcg                                                     18


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: PykA-FOR2

<400>  33
cgaagcggtt aatctgctg                                                    19


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: PykF-FOR1

<400>  34
cggctcatca gttggtactg                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide: PykF-FOR2

<400>  35
gggtaaagaa ctggctctgc                                                   20


<210>  36
<211>  3527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tetA-sacB Cassette

<400>  36
tcctaatttt tgttgacact ctatcattga tagagttatt ttaccactcc ctatcagtga       60

tagagaaaag tgaaatgaat agttcgacaa agatcgcatt ggtaattacg ttactcgatg      120

ccatggggat tggccttatc atgccagtct tgccaacgtt attacgtgaa tttattgctt      180

cggaagatat cgctaaccac tttggcgtat tgcttgcact ttatgcgtta atgcaggtta      240

tctttgctcc ttggcttgga aaaatgtctg accgatttgg tcggcgccca gtgctgttgt      300

tgtcattaat aggcgcatcg ctggattact tattgctggc tttttcaagt gcgctttgga      360

tgctgtattt aggccgtttg ctttcaggga tcacaggagc tactggggct gtcgcggcat      420

cggtcattgc cgataccacc tcagcttctc aacgcgtgaa gtggttcggt tggttagggg      480

caagttttgg gcttggttta atagcggggc ctattattgg tggttttgca ggagagattt      540

caccgcatag tccctttttt atcgctgcgt tgctaaatat tgtcactttc cttgtggtta      600

tgttttggtt ccgtgaaacc aaaaatacac gtgataatac agataccgaa gtaggggttg      660

agacgcaatc gaattcggta tacatcactt tatttaaaac gatgcccatt ttgttgatta      720

tttatttttc agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg ctatttaccg      780

aaaatcgttt tggatggaat agcatgatgg ttggcttttc attagcgggt cttggtcttt      840

tacactcagt attccaagcc tttgtggcag gaagaatagc cactaaatgg ggcgaaaaaa      900

cggcagtact gctcggattt attgcagata gtagtgcatt tgccttttta gcgtttatat      960

ctgaaggttg gttagttttc cctgttttaa ttttattggc tggtggtggg atcgctttac     1020

ctgcattaca gggagtgatg tctatccaaa caaagagtca tcagcaaggt gctttacagg     1080

gattattggt gagccttacc aatgcaaccg gtgttattgg cccattactg tttgctgtta     1140

tttataatca ttcactacca atttgggatg gctggatttg gattattggt ttagcgtttt     1200

actgtattat tatcctgcta tcgatgacct tcatgttaac ccctcaagct caggggagta     1260

aacaggagac aagtgcttag ttatttcgtc accaaatgat gttattccgc gaaatataat     1320

gaccctcttg ataacccaag agcatcacat atacctgccg ttcactatta tttagtgaaa     1380

tgagatatta tgatattttc tgaattgtga ttaaaaaggc aactttatgc ccatgcaaca     1440

gaaactataa aaaatacaga gaatgaaaag aaacagatag attttttagt tctttaggcc     1500

cgtagtctgc aaatcctttt atgattttct atcaaacaaa agaggaaaat agaccagttg     1560

caatccaaac gagagtctaa tagaatgagg tcgaaaagta aatcgcgcgg gtttgttact     1620

gataaagcag gcaagaccta aaatgtgtaa agggcaaagt gtatactttg gcgtcacccc     1680

ttacatattt taggtctttt tttattgtgc gtaactaact tgccatcttc aaacaggagg     1740

gctggaagaa gcagaccgct aacacagtac ataaaaaagg agacatgaac gatgaacatc     1800

aaaaagtttg caaaacaagc aacagtatta acctttacta ccgcactgct ggcaggaggc     1860

gcaactcaag cgtttgcgaa agaaacgaac caaaagccat ataaggaaac atacggcatt     1920

tcccatatta cacgccatga tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat     1980

caagttcctg agttcgattc gtccacaatt aaaaatatct cttctgcaaa aggcctggac     2040

gtttgggaca gctggccatt acaaaacgct gacggcactg tcgcaaacta tcacggctac     2100

cacatcgtct ttgcattagc cggagatcct aaaaatgcgg atgacacatc gatttacatg     2160

ttctatcaaa aagtcggcga aacttctatt gacagctgga aaaacgctgg ccgcgtcttt     2220

aaagacagcg acaaattcga tgcaaatgat tctatcctaa aagaccaaac acaagaatgg     2280

tcaggttcag ccacatttac atctgacgga aaaatccgtt tattctacac tgatttctcc     2340

ggtaaacatt acggcaaaca aacactgaca actgcacaag ttaacgtatc agcatcagac     2400

agctctttga acatcaacgg tgtagaggat tataaatcaa tctttgacgg tgacggaaaa     2460

acgtatcaaa atgtacagca gttcatcgat gaaggcaact acagctcagg cgacaaccat     2520

acgctgagag atcctcacta cgtagaagat aaaggccaca aatacttagt atttgaagca     2580

aacactggaa ctgaagatgg ctaccaaggc gaagaatctt tatttaacaa agcatactat     2640

ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac ttctgcaaag cgataaaaaa     2700

cgcacggctg agttagcaaa cggcgctctc ggtatgattg agctaaacga tgattacaca     2760

ctgaaaaaag tgatgaaacc gctgattgca tctaacacag taacagatga aattgaacgc     2820

gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca ctgactcccg cggatcaaaa     2880

atgacgattg acggcattac gtctaacgat atttacatgc ttggttatgt ttctaattct     2940

ttaactggcc catacaagcc gctgaacaaa actggccttg tgttaaaaat ggatcttgat     3000

cctaacgatg taacctttac ttactcacac ttcgctgtac ctcaagcgaa aggaaacaat     3060

gtcgtgatta caagctatat gacaaacaga ggattctacg cagacaaaca atcaacgttt     3120

gcgccaagct tcctgctgaa catcaaaggc aagaaaacat ctgttgtcaa agacagcatc     3180

cttgaacaag gacaattaac agttaacaaa taaaaacgca aaagaaaatg ccgatattga     3240

ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg attcaggctg tctatgtgtg     3300

actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt tgctttgttt     3360

tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca ttgtcgatct     3420

gttcatggtg aacagcttta aatgcaccaa aaactcgtaa aagctctgat gtatctatct     3480

tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgat                   3527


<210>  37
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   delta-iclR-cure

<400>  37
aaatgatttc cacgatacag aaaaaagaga ctgtcatggg cagaatattg cctctgcccg       60

ccagaaaaag                                                              70


<210>  38
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta-arcA-cure

<400>  38
ctgtttcgat ttagttggca atttaggtag caaactcggc tttaccaccg tcaaaaaaaa       60

cggcgctttt                                                              70


<210>  39
<211>  974
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta-cas3::ugBp-sspB-pro-casA

<400>  39
caagacatgt gtatatcact gtaattcgat atttatgagc agcatcgaaa aatagcccgc       60

tgatatcatc gataatacta aaaaaacagg gaggctatta ccaggcatca aataaaacga      120

aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc      180

tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat ctttctgaca      240

ccttactatc ttacaaatgt aacaaaaaag ttatttttct gtaattcgag catgtcatgt      300

taccccgcga gcataaaacg cgtgtgtagg aggataatct atggatttgt cacagctaac      360

accacgtcgt ccctatctgc tgcgtgcatt ctatgagtgg ttgctggata accagctcac      420

gccgcacctg gtggtggatg tgacgctccc tggcgtgcag gttcctatgg aatatgcgcg      480

tgacgggcaa atcgtactca acattgcgcc gcgtgctgtc ggcaatctgg aactggcgaa      540

tgatgaggtg cgctttaacg cgcgctttgg tggcattccg cgtcaggttt ctgtgccgct      600

ggctgccgtg ctggctatct acgcccgtga aaatggcgca ggcacgatgt ttgagcctga      660

agctgcctac gatgaagata ccagcatcat gaatgatgaa gaggcatcgg cagacaacga      720

aaccgttatg tcggttattg atggcgacaa gccagatcac gatgatgaca ctcatcctga      780

cgatgaacct ccgcagccac cacgcggtgg tcgaccggca ttacgcgttg tgaagtaatt      840

gacggctagc tcagtcctag gtacagtgct agccatatga aggagaacaa atgaatttgc      900

ttattgataa ctggatccct gtacgcccgc gaaacggggg gaaagtccaa atcataaatc      960

tgcaatcgct atac                                                        974


<210>  40
<211>  1421
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gltA-DAS+4-ampR

<400>  40
gtattccgtc ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg       60

cccactggag cgaaatgcac agtgacggta tgaagattgc ccgtccgcgt cagctgtata      120

caggatatga aaaacgcgac tttaaaagcg atatcaagcg tgcggccaac gatgaaaact      180

attctgaaaa ctatgcggat gcgtcttaat agtcctgacg gatggccttt ttgcgtttct      240

acaaactctt tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa      300

taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc      360

cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa      420

acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa      480

ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg      540

atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa      600

gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc      660

acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc      720

atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta      780

accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag      840

ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctacagc aatggcaaca      900

acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata      960

gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc     1020

tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca     1080

ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca     1140

actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg     1200

taactgtcag actaatggtt gattgctaag ttgtaaatat tttaacccgc cgttcatatg     1260

gcgggttgat ttttatatgc ctaaacacaa aaaattgtaa aaataaaatc cattaacaga     1320

cctatataga tatttaaaaa gaatagaaca gctcaaatta tcagcaaccc aatactttca     1380

attaaaaact tcatggtagt cgcatttata accctatgaa a                         1421


<210>  41
<211>  1078
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gltA-DAS+4-purR

<400>  41
accgtcattt tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac       60

agtgacggta tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac      120

tttaaaagcg atatcaagcg tgcggccaac gatgaaaact attctgaaaa ctatgcggat      180

gcgtcttaat cctgacggat ggcctttttg cgtttctaca aactcttttt gtttattttt      240

ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata      300

atattgaaaa aggaagagta tgactgaata caagcccacg gtacgcttgg cgacgcgcga      360

cgatgttccc cgcgctgttc gtacattagc tgcggccttt gcagattacc cagcgacgcg      420

ccatacggtc gatccggacc gccatatcga gcgtgtcaca gaattgcagg aacttttctt      480

aactcgcgtg ggccttgaca tcggaaaggt ctgggtggct gacgatggcg ctgcagtggc      540

tgtttggacc actccggaga gtgtagaggc tggtgcagtg ttcgccgaaa ttggtcctcg      600

tatggccgaa ttaagtggaa gtcgtctggc agcccaacaa caaatggaag ggttgcttgc      660

gccccaccgt ccgaaagaac ccgcgtggtt ccttgccacc gttggagtaa gcccagatca      720

ccaggggaag ggtttaggat ctgccgtagt tttaccaggt gtggaggcag cagaacgtgc      780

gggagttccg gccttccttg agacgtcggc gccgcgcaat ttaccgtttt acgaacgtct      840

tggattcacc gttacggcgg acgtggaggt gccggaggga ccccgtactt ggtgtatgac      900

tcgtaaaccg ggagcctgat aatggttgat tgctaagttg taaatatttt aacccgccgt      960

tcatatggcg ggttgatttt tatatgccta aacacaaaaa attgtaaaaa taaaatccat     1020

taacagacct atatagatat ttaaaaagaa tagaacagct caaattatca gcaaccca       1078


<210>  42
<211>  869
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gltA-DAS+4-zeoR

<400>  42
gtattccgtc ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg       60

cccactggag cgaaatgcac agtgacggta tgaagattgc ccgtccgcgt cagctgtata      120

caggatatga aaaacgcgac tttaaaagcg atatcaagcg tgcggccaac gatgaaaact      180

attctgaaaa ctatgcggat gcgtcttaat agttgacaat taatcatcgg catagtatat      240

cggcatagta taatacgact cactatagga gggccatcat ggccaagttg accagtgccg      300

ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg      360

ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc      420

tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg      480

tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg      540

acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc      600

tgcgcgaccc ggccggcaac tgcgtgcact ttgtggcaga ggagcaggac tgaggataag      660

taatggttga ttgctaagtt gtaaatattt taacccgccg ttcatatggc gggttgattt      720

ttatatgcct aaacacaaaa aattgtaaaa ataaaatcca ttaacagacc tatatagata      780

tttaaaaaga atagaacagc tcaaattatc agcaacccaa tactttcaat taaaaacttc      840

atggtagtcg catttataac cctatgaaa                                        869


<210>  43
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lpd-DAS+4-gentR

<400>  43
gcggcgagct gctgggtgaa atcggcctgg caatcgaaat gggttgtgat gctgaagaca       60

tcgcactgac catccacgcg cacccgactc tgcacgagtc tgtgggcctg gcggcagaag      120

tgttcgaagg tagcattacc gacctgccga acccgaaagc gaagaagaag gcggccaacg      180

atgaaaacta ttctgaaaac tatgcggatg cgtcttaata gcgaatccat gtgggagttt      240

attcttgaca cagatattta tgatataata actgagtaag cttaacataa ggaggaaaaa      300

catatgttac gcagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta      360

ggtggctcaa gtatgggcat cattcgcaca tgtaggctcg gccctgacca agtcaaatcc      420

atgcgggctg ctcttgatct tttcggtcgt gagttcggag acgtagccac ctactcccaa      480

catcagccgg actccgatta cctcgggaac ttgctccgta gtaagacatt catcgcgctt      540

gctgccttcg accaagaagc ggttgttggc gctctcgcgg cttacgttct gcccaagttt      600

gagcagccgc gtagtgagat ctatatctat gatctcgcag tctccggcga gcaccggagg      660

cagggcattg ccaccgcgct catcaatctc ctcaagcatg aggccaacgc gcttggtgct      720

tatgtgatct acgtgcaagc agattacggt gacgatcccg cagtggctct ctatacaaag      780

ttgggcatac gggaagaagt gatgcacttt gatatcgacc caagtaccgc cacctaattt      840

ttcgtttgcc ggaacatccg gcaattaaaa aagcggctaa ccacgccgct ttttttacgt      900

ctgcaattta cctttccagt cttcttgctc cacgttcaga gagacgttcg catactgctg      960

accgttgctc gttattcagc ctgacagtat ggttactgtc                           1000


<210>  44
<211>  898
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  zwf-DAS+4-bsdR

<400>  44
gaagtggaag aagcctggaa atgggtagac tccattactg aggcgtgggc gatggacaat       60

gatgcgccga aaccgtatca ggccggaacc tggggacccg ttgcctcggt ggcgatgatt      120

acccgtgatg gtcgttcctg gaatgagttt gaggcggcca acgatgaaaa ctattctgaa      180

aactatgcgg atgcgtctta atagttgaca attaatcatc ggcatagtat atcggcatag      240

tataatacga ctcactatag gagggccatc atgaagacct tcaacatctc tcagcaggat      300

ctggagctgg tggaggtcgc cactgagaag atcaccatgc tctatgagga caacaagcac      360

catgtcgggg cggccatcag gaccaagact ggggagatca tctctgctgt ccacattgag      420

gcctacattg gcagggtcac tgtctgtgct gaagccattg ccattgggtc tgctgtgagc      480

aacgggcaga aggactttga caccattgtg gctgtcaggc acccctactc tgatgaggtg      540

gacagatcca tcagggtggt cagcccctgt ggcatgtgca gagagctcat ctctgactat      600

gctcctgact gctttgtgct cattgagatg aatggcaagc tggtcaaaac caccattgag      660

gaactcatcc ccctcaagta caccaggaac taaagtaata tctgcgctta tcctttatgg      720

ttattttacc ggtaacatga tcttgcgcag attgtagaac aatttttaca ctttcaggcc      780

tcgtgcggat tcacccacga ggcttttttt attacactga ctgaaacgtt tttgccctat      840

gagctccggt tacaggcgtt tcagtcataa atcctctgaa tgaaacgcgt tgtgaatc        898


<210>  45
<211>  1454
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pykA-DAS+4:ampR

<400>  45
tgaacctgac tgctctctat cgtggcgtta cgccggtgca ctttgatagc gctaatgacg       60

gcgtagcagc tgccagcgaa gcggttaatc tgctgcgcga taaaggttac ttgatgtctg      120

gtgacctggt gattgtcacc cagggcgacg tgatgagtac cgtgggttct actaatacca      180

cgcgtatttt aacggtagag gcggccaacg atgaaaacta ttctgaaaac tatgcggatg      240

cgtcttaata gtcctgacgg atggcctttt tgcgtttcta caaactcttt ttgtttattt      300

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa      360

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt      420

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat      480

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag      540

atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg      600

ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata      660

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat      720

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc      780

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg      840

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac      900

gacgagcgtg acaccacgat gcctacagca atggcaacaa cgttgcgcaa actattaact      960

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa     1020

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct     1080

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc     1140

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga     1200

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ctaagtacgt     1260

tgccggatgc ggcgaaaacg ccacatccgg cctacagttc aatgatagtt caacagattt     1320

cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc     1380

ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc     1440

gagttttcag caat                                                       1454


<210>  46
<211>  1310
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pykF-DAS+4:purr

<400>  46
aaacggctca tcagttggta ctgagcaaag gcgttgtgcc gcagcttgtt aaagagatca       60

cttctactga tgatttctac cgtctgggta aagaactggc tctgcagagc ggtctggcac      120

acaaaggtga cgttgtagtt atggtttctg gtgcactggt accgagcggc actactaaca      180

ccgcatctgt tcacgtcctg gcggccaacg atgaaaacta ttctgaaaac tatgcggatg      240

cgtcttaatc ctgacggatg gcctttttgc gtttctacaa actctttttg tttatttttc      300

taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa      360

tattgaaaaa ggaagagtat gactgaatac aagcccacgg tacgcttggc gacgcgcgac      420

gatgttcccc gcgctgttcg tacattagct gcggcctttg cagattaccc agcgacgcgc      480

catacggtcg atccggaccg ccatatcgag cgtgtcacag aattgcagga acttttctta      540

actcgcgtgg gccttgacat cggaaaggtc tgggtggctg acgatggcgc tgcagtggct      600

gtttggacca ctccggagag tgtagaggct ggtgcagtgt tcgccgaaat tggtcctcgt      660

atggccgaat taagtggaag tcgtctggca gcccaacaac aaatggaagg gttgcttgcg      720

ccccaccgtc cgaaagaacc cgcgtggttc cttgccaccg ttggagtaag cccagatcac      780

caggggaagg gtttaggatc tgccgtagtt ttaccaggtg tggaggcagc agaacgtgcg      840

ggagttccgg ccttccttga gacgtcggcg ccgcgcaatt taccgtttta cgaacgtctt      900

ggattcaccg ttacggcgga cgtggaggtg ccggagggac cccgtacttg gtgtatgact      960

cgtaaaccgg gagcctgata atattgcttt tgtgaattaa tttgtatatc gaagcgccct     1020

gatgggcgct ttttttattt aatcgataac cagaagcaat aaaaaatcaa atcggatttc     1080

actatataat ctcactttat ctaagatgaa tccgatggaa gcatcctgtt ttctctcaat     1140

ttttttatct aaaacccagc gttcgatgct tctttgagcg aacgatcaaa aataagtgcc     1200

ttcccatcaa aaaaatattc tcaacataaa aaactttgtg taatacttgt aacgctacat     1260

ggagattaac tcaatctaga gggtattaat aatgaaagct actaaactgg                1310


<210>  47
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer:  AM512_gRNAmgsA

<400>  47
ctgtatgcaa caggcactac gttttagagc tagaaatagc aag                         43


<210>  48
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM225_sgRNA_F3

<400>  48
gtgctcagta tctctatcac tga                                               23


<210>  49
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM514_gRNAptsA

<400>  49
atgtgttctg atttgctgtg gttttagagc tagaaatagc aag                         43


<210>  50
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM225_sgRNA_F3

<400>  50
gtgctcagta tctctatcac tga                                               23


<210>  51
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM536_gRNAepd

<400>  51
taaatggctt cggtcgcatc gttttagagc tagaaatagc aag                         43


<210>  52
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM225_sgRNA_F3

<400>  52
gtgctcagta tctctatcac tga                                               23


<210>  53
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AM537_gRNAdhaL

<400>  53
ctttatcaga tgttccgcga gttttagagc tagaaatagc aag                         43


<210>  54
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM225_sgRNA_F3

<400>  54
gtgctcagta tctctatcac tga                                               23


<210>  55
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM550_gRNAeda

<400>  55
ggtgctgaat ccacagcagc gttttagagc tagaaatagc aag                         43


<210>  56
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM225_sgRNA_F3

<400>  56
gtgctcagta tctctatcac tga                                               23


<210>  57
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AM519_D ONmgsA

<400>  57
gcagcataag tgcttacagt aatctgtagg aaagttaact acggatcccc gattatcagc       60

gttatctcgc ggaccgtctg aagtaa                                            86


<210>  58
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AM549_D ONPtsA2

<400>  58
ccttttacag ttccagttca tgttgcagca ggctggcgat agcgttttgc ggcatgcttc       60

cggtttatcg caagttatga ggcggatcgc                                        90


<210>  59
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AM538_D ONepd

<400>  59
ccttttacag ttccagttca tgttgcagca ggctggcgat agcgttttgc ggcatgcttc       60

cggtttatcg caagttatga ggcggatcgc                                        90


<210>  60
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AM539_D ONdhaL

<400>  60
gattgactat cgaacgtaat ttaattggcg cgtactgcac ctcacgactg ggagaaggtg       60

tcggtgaatt agcccgtcag atgttaatga                                        90


<210>  61
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AM551_D ONeda

<400>  61
gccttctaca gcttcacgcg ccagcttagt aatgcggtcg taatcgcctg attacaaatt       60

tgtcgtctta aaaagtgata caggttgcgc                                        90


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM525_byemgsA_F

<400>  62
ctgacccaca aacgcgaaat                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM526_byemgsA_R

<400>  63
ggtggcgaga aaaccgtaag                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM547_byeptsA_F2

<400>  64
cgcccgtcat taaatgctga                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM548_byeptsA_R2

<400>  65
ggctaataac ccttgtgcgg                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM540_byeEpd_F

<400>  66
ttcggctgga caaacattcc                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AM541_byeEpd_R

<400>  67
aacctgttga tcgtgcatgg                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM542_byeDhaL_F

<400>  68
cgtctataac cgcctgacca                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM543_byeDhaL_R

<400>  69
ttgtggatcg tcaattcccg                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM552_byeEda_F

<400>  70
ctggtagacg aagcggaact                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer: AM553_byeEda_R

<400>  71
cctcgatcgg gcattttgac                                                   20


<210>  72
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA

<400>  72
tcgagttccc cgcgccagcg gggataaacc gtgacgatcg ctaaaaacga ctgtcactgt       60

ctcgagttcc ccgcgccagc ggggataaac cg                                     92


<210>  73
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA-sgRNA-FOR

<400>  73
acgactgtca ctgtctcgag ttccccgcgc cagcggggat aaaccgaaaa aaaaacccc        59


<210>  74
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA-sgRNA-REV

<400>  74
ttttagcgat cgtcacggtt tatccccgct ggcgcgggga actcgaggtg gtaccagatc       60


<210>  75
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykF

<400>  75
tcgagttccc cgcgccagcg gggataaacc gcaccaccac tttcgtaata ccggattcgc       60

ttcgagttcc ccgcgccagc ggggataaac cg                                     92


<210>  76
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykF-sgRNA-FOR

<400>  76
aataccggat tcgcttcgag ttccccgcgc cagcggggat aaaccgaaaa aaaaacccc        59


<210>  77
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykF-sgRNA-REV

<400>  77
acgaaagtgg tggtgcggtt tatccccgct ggcgcgggga actcgaggtg gtaccagatc       60


<210>  78
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA-pykF

<400>  78
tcgagttccc cgcgccagcg gggataaacc gtgacgatcg ctaaaaacga ctgtcactgt       60

ctcgagttcc ccgcgccagc ggggataaac cgcaccacca ctttcgtaat accggattcg      120

cttcgagttc cccgcgccag cggggataaa ccg                                   153


<210>  79
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykF-FOR

<400>  79
aataccggat tcgcttcgag ttccccgcgc cagcggggat aaaccgaaaa aaaaacccc        59


<210>  80
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pCASCADE-REV

<400>  80
cttgcccgcc tgatgaatgc tcatccgg                                          28


<210>  81
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pCASCADE-FOR

<400>  81
ccggatgagc attcatcagg cgggcaag                                          28


<210>  82
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA-REV

<400>  82
acgaaagtgg tggtgcggtt tatccccgct ggcgcgggga actcgaggtg gtaccagatc       60


<210>  83
<211>  215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: gltA1-pykA-pykF

<400>  83
tcgagttccc cgcgccagcg gggataaacc gaaaagcata taatgcgtaa aagttatgaa       60

gttcgagttc cccgcgccag cggggataaa ccgtgacgat cgctaaaaac gactgtcact      120

gtctcgagtt ccccgcgcca gcggggataa accgcaccac cactttcgta ataccggatt      180

cgcttcgagt tccccgcgcc agcggggata aaccg                                 215


<210>  84
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA-FOR

<400>  84
gcgccagcgg ggataaaccg tgacgatcgc taaaaac                                37


<210>  85
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name:  gltA1-REV

<400>  85
cggtttatcc ccgctggcgc ggggaactcg aacttcataa cttttac                     47


<210>  86
<211>  216
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: gltA2-pykA-pykF

<400>  86
tcgagttccc cgcgccagcg gggataaacc gtattgacca attcattcgg gacagttatt       60

agttcgagtt ccccgcgcca gcggggataa accgtgacga tcgctaaaaa cgactgtcac      120

tgtctcgagt tccccgcgcc agcggggata aaccgcacca ccactttcgt aataccggat      180

tcgcttcgag ttccccgcgc cagcggggat aaaccg                                216


<210>  87
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name:pykA-FOR

<400>  87
gcgccagcgg ggataaaccg tgacgatcgc taaaaac                                37


<210>  88
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name:gltA2-REV

<400>  88
cggtttatcc ccgctggcgc ggggaactcg aactaataac tgtc                        44


<210>  89
<211>  214
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: zwf-pykA-pykF

<400>  89
tcgagttccc cgcgccagcg gggataaacc gctcgtaaaa gcagtacagt gcaccgtaag       60

atcgagttcc ccgcgccagc ggggataaac cgtgacgatc gctaaaaacg actgtcactg      120

tctcgagttc cccgcgccag cggggataaa ccgcaccacc actttcgtaa taccggattc      180

gcttcgagtt ccccgcgcca gcggggataa accg                                  214


<210>  90
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA-FOR

<400>  90
gcgccagcgg ggataaaccg tgacgatcgc taaaaac                                37


<210>  91
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name:  zwf-REV

<400>  91
cggtttatcc ccgctggcgc ggggaactcg atcttacggt gcactgtac                   49


<210>  92
<211>  278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: gltA1-gltA2-pykA-pykF

<400>  92
tcgagttccc cgcgccagcg gggataaacc gaaaagcata taatgcgtaa aagttatgaa       60

gttcgagttc cccgcgccag cggggataaa ccgtattgac caattcattc gggacagtta      120

ttagttcgag ttccccgcgc cagcggggat aaaccgtgac gatcgctaaa aacgactgtc      180

actgtctcga gttccccgcg ccagcgggga taaaccgcac caccactttc gtaataccgg      240

attcgcttcg agttccccgc gccagcgggg ataaaccg                              278


<210>  93
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name:  pykA-FOR

<400>  93
gcgccagcgg ggataaaccg tgacgatcgc taaaaac                                37


<210>  94
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: gltA2-REV

<400>  94
cggtttatcc ccgctggcgc ggggaactcg aactaataac tgtc                        44


<210>  95
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: gltA1-zwf-pykA-pykF

<400>  95
tcgagttccc cgcgccagcg gggataaacc gaaaagcata taatgcgtaa aagttatgaa       60

gttcgagttc cccgcgccag cggggataaa ccgctcgtaa aagcagtaca gtgcaccgta      120

agatcgagtt ccccgcgcca gcggggataa accgtgacga tcgctaaaaa cgactgtcac      180

tgtctcgagt tccccgcgcc agcggggata aaccgcacca ccactttcgt aataccggat      240

tcgcttcgag ttccccgcgc cagcggggat aaaccg                                276


<210>  96
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA-FOR

<400>  96
gcgccagcgg ggataaaccg tgacgatcgc taaaaac                                37


<210>  97
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name:zwf-REV

<400>  97
cggtttatcc ccgctggcgc ggggaactcg atcttacggt gcactgtac                   49


<210>  98
<211>  277
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: gltA2-zwf-pykA-pykF

<400>  98
tcgagttccc cgcgccagcg gggataaacc gtattgacca attcattcgg gacagttatt       60

agttcgagtt ccccgcgcca gcggggataa accgctcgta aaagcagtac agtgcaccgt      120

aagatcgagt tccccgcgcc agcggggata aaccgtgacg atcgctaaaa acgactgtca      180

ctgtctcgag ttccccgcgc cagcggggat aaaccgcacc accactttcg taataccgga      240

ttcgcttcga gttccccgcg ccagcgggga taaaccg                               277


<210>  99
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name:pykA-FOR

<400>  99
gcgccagcgg ggataaaccg tgacgatcgc taaaaac                                37


<210>  100
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: zwf-REV

<400>  100
cggtttatcc ccgctggcgc ggggaactcg atcttacggt gcactgtac                   49


<210>  101
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name:gltA1-gltA2-zwf-pykA-pykF

<400>  101
tcgagttccc cgcgccagcg gggataaacc gaaaagcata taatgcgtaa aagttatgaa       60

gttcgagttc cccgcgccag cggggataaa ccgtattgac caattcattc gggacagtta      120

ttagttcgag ttccccgcgc cagcggggat aaaccgctcg taaaagcagt acagtgcacc      180

gtaagatcga gttccccgcg ccagcgggga taaaccgtga cgatcgctaa aaacgactgt      240

cactgtctcg agttccccgc gccagcgggg ataaaccgca ccaccacttt cgtaataccg      300

gattcgcttc gagttccccg cgccagcggg gataaaccg                             339


<210>  102
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: pykA-FOR

<400>  102
gcgccagcgg ggataaaccg tgacgatcgc taaaaac                                37


<210>  103
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA/Primer name: zwf-REV

<400>  103
cggtttatcc ccgctggcgc ggggaactcg atcttacggt gcactgtac                   49


