                         SEQUENCE LISTING

<110>  UCB Biopharma SPRL
       Oxford University Innovation Limited
       The University of Adelaide
 
<120>  GREMLIN-1 ANTAGONIST FOR THE PREVENTION AND TREATMENT OF CANCER

<130>  N412703WO

<150>  GB 1809946.5
<151>  2018-06-18

<150>  GB 1815694.3
<151>  2018-09-26

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  184
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ser Arg Thr Ala Tyr Thr Val Gly Ala Leu Leu Leu Leu Leu Gly 
1               5                   10                  15      


Thr Leu Leu Pro Ala Ala Glu Gly Lys Lys Lys Gly Ser Gln Gly Ala 
            20                  25                  30          


Ile Pro Pro Pro Asp Lys Ala Gln His Asn Asp Ser Glu Gln Thr Gln 
        35                  40                  45              


Ser Pro Gln Gln Pro Gly Ser Arg Asn Arg Gly Arg Gly Gln Gly Arg 
    50                  55                  60                  


Gly Thr Ala Met Pro Gly Glu Glu Val Leu Glu Ser Ser Gln Glu Ala 
65                  70                  75                  80  


Leu His Val Thr Glu Arg Lys Tyr Leu Lys Arg Asp Trp Cys Lys Thr 
                85                  90                  95      


Gln Pro Leu Lys Gln Thr Ile His Glu Glu Gly Cys Asn Ser Arg Thr 
            100                 105                 110         


Ile Ile Asn Arg Phe Cys Tyr Gly Gln Cys Asn Ser Phe Tyr Ile Pro 
        115                 120                 125             


Arg His Ile Arg Lys Glu Glu Gly Ser Phe Gln Ser Cys Ser Phe Cys 
    130                 135                 140                 


Lys Pro Lys Lys Phe Thr Thr Met Met Val Thr Leu Asn Cys Pro Glu 
145                 150                 155                 160 


Leu Gln Pro Pro Thr Lys Lys Lys Arg Val Thr Arg Val Lys Gln Cys 
                165                 170                 175     


Arg Cys Ile Ser Ile Asp Leu Asp 
            180                 


<210>  2
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human truncated Gremlin-1 used in crystallography with N-terminal
       tag

<400>  2

Met Gly Ser Ser His His His His His His Ser Ser Gly Glu Asn Leu 
1               5                   10                  15      


Tyr Phe Gln Gly Ser Ala Met Pro Gly Glu Glu Val Leu Glu Ser Ser 
            20                  25                  30          


Gln Glu Ala Leu His Val Thr Glu Arg Lys Tyr Leu Lys Arg Asp Trp 
        35                  40                  45              


Cys Lys Thr Gln Pro Leu Lys Gln Thr Ile His Glu Glu Gly Cys Asn 
    50                  55                  60                  


Ser Arg Thr Ile Ile Asn Arg Phe Cys Tyr Gly Gln Cys Asn Ser Phe 
65                  70                  75                  80  


Tyr Ile Pro Arg His Ile Arg Lys Glu Glu Gly Ser Phe Gln Ser Cys 
                85                  90                  95      


Ser Phe Cys Lys Pro Lys Lys Phe Thr Thr Met Met Val Thr Leu Asn 
            100                 105                 110         


Cys Pro Glu Leu Gln Pro Pro Thr Lys Lys Lys Arg Val Thr Arg Val 
        115                 120                 125             


Lys Gln Cys Arg Cys Ile Ser Ile Asp Leu Asp 
    130                 135                 


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 HCDR1 combined Kabat & Chothia

<400>  3

Gly Tyr Thr Phe Thr Asp Tyr Tyr Met His 
1               5                   10  


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 HCDR1 Kabat

<400>  4

Asp Tyr Tyr Met His 
1               5   


<210>  5
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 HCDR2 Kabat

<400>  5

Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 HCDR3 Kabat

<400>  6

Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp Tyr 
1               5                   10                  15  


<210>  7
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 LCDR1 Kabat

<400>  7

Lys Ser Ser Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 LCDR2 Kabat

<400>  8

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 LCDR3 Kabat

<400>  9

Gln Gln Tyr Tyr Asp Thr Pro Thr 
1               5               


<210>  10
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 Heavy chain variable region variant 1

<400>  10

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  11
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 Light chain variable region variant 1

<400>  11

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


<210>  12
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 Heavy chain variable region variant 2

<400>  12

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  13
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab7326 Light chain variable region variant 2

<400>  13

Asp Ile Val Met Thr Gln Thr Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


<210>  14
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse full length IgG1 heavy chain variant 1

<400>  14

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Lys Thr Thr 
        115                 120                 125             


Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn 
    130                 135                 140                 


Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val 
            180                 185                 190         


Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val 
        195                 200                 205             


Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg 
    210                 215                 220                 


Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser 
225                 230                 235                 240 


Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu 
                245                 250                 255     


Thr Pro Lys Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro 
            260                 265                 270         


Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala 
        275                 280                 285             


Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val 
    290                 295                 300                 


Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe 
305                 310                 315                 320 


Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile 
            340                 345                 350         


Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys 
        355                 360                 365             


Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp 
    370                 375                 380                 


Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp 
385                 390                 395                 400 


Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser 
                405                 410                 415     


Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly 
            420                 425                 430         


Leu His Asn His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
        435                 440                 445             


<210>  15
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse full length IgG1 light chain variant 1

<400>  15

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
    210                 215                 


<210>  16
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human full length IgG1 heavy chain variant 2

<400>  16

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120                 125             


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
    130                 135                 140                 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
            180                 185                 190         


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
        195                 200                 205             


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
    210                 215                 220                 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
225                 230                 235                 240 


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
                245                 250                 255     


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
            260                 265                 270         


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
        275                 280                 285             


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
    290                 295                 300                 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
305                 310                 315                 320 


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
                325                 330                 335     


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
            340                 345                 350         


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
        355                 360                 365             


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
    370                 375                 380                 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
385                 390                 395                 400 


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
                405                 410                 415     


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
            420                 425                 430         


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
        435                 440                 445             


Ser Leu Ser Pro Gly Lys 
    450                 


<210>  17
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human full length IgG1 light chain variant 2

<400>  17

Asp Ile Val Met Thr Gln Thr Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  18
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fab heavy chain variant 1

<400>  18

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120                 125             


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
    130                 135                 140                 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
            180                 185                 190         


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
        195                 200                 205             


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
    210                 215                 220                 


Lys Ser Cys 
225         


<210>  19
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fab light chain variant 1

<400>  19

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  20
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human truncated Gremlin-1 used in crystallography without 
       N-terminal tag

<400>  20

Ala Met Pro Gly Glu Glu Val Leu Glu Ser Ser Gln Glu Ala Leu His 
1               5                   10                  15      


Val Thr Glu Arg Lys Tyr Leu Lys Arg Asp Trp Cys Lys Thr Gln Pro 
            20                  25                  30          


Leu Lys Gln Thr Ile His Glu Glu Gly Cys Asn Ser Arg Thr Ile Ile 
        35                  40                  45              


Asn Arg Phe Cys Tyr Gly Gln Cys Asn Ser Phe Tyr Ile Pro Arg His 
    50                  55                  60                  


Ile Arg Lys Glu Glu Gly Ser Phe Gln Ser Cys Ser Phe Cys Lys Pro 
65                  70                  75                  80  


Lys Lys Phe Thr Thr Met Met Val Thr Leu Asn Cys Pro Glu Leu Gln 
                85                  90                  95      


Pro Pro Thr Lys Lys Lys Arg Val Thr Arg Val Lys Gln Cys Arg Cys 
            100                 105                 110         


Ile Ser Ile Asp Leu Asp 
        115             


<210>  21
<211>  160
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature Gremlin-1 sequence of SEQ ID NO: 1 lacking the signal 
       peptide of amino acids 1-21

<400>  21

Lys Lys Lys Gly Ser Gln Gly Ala Ile Pro Pro Pro Asp Lys Ala Gln 
1               5                   10                  15      


His Asn Asp Ser Glu Gln Thr Gln Ser Pro Gln Gln Pro Gly Ser Arg 
            20                  25                  30          


Asn Arg Gly Arg Gly Gln Gly Arg Gly Thr Ala Met Pro Gly Glu Glu 
        35                  40                  45              


Val Leu Glu Ser Ser Gln Glu Ala Leu His Val Thr Glu Arg Lys Tyr 
    50                  55                  60                  


Leu Lys Arg Asp Trp Cys Lys Thr Gln Pro Leu Lys Gln Thr Ile His 
65                  70                  75                  80  


Glu Glu Gly Cys Asn Ser Arg Thr Ile Ile Asn Arg Phe Cys Tyr Gly 
                85                  90                  95      


Gln Cys Asn Ser Phe Tyr Ile Pro Arg His Ile Arg Lys Glu Glu Gly 
            100                 105                 110         


Ser Phe Gln Ser Cys Ser Phe Cys Lys Pro Lys Lys Phe Thr Thr Met 
        115                 120                 125             


Met Val Thr Leu Asn Cys Pro Glu Leu Gln Pro Pro Thr Lys Lys Lys 
    130                 135                 140                 


Arg Val Thr Arg Val Lys Gln Cys Arg Cys Ile Ser Ile Asp Leu Asp 
145                 150                 155                 160 


<210>  22
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4P heavy chain variant 1

<400>  22

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120                 125             


Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu 
    130                 135                 140                 


Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
            180                 185                 190         


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn 
        195                 200                 205             


Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser 
    210                 215                 220                 


Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly 
225                 230                 235                 240 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
                245                 250                 255     


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
            260                 265                 270         


Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
        275                 280                 285             


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr 
    290                 295                 300                 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
305                 310                 315                 320 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile 
                325                 330                 335     


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
            340                 345                 350         


Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser 
        355                 360                 365             


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
    370                 375                 380                 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
385                 390                 395                 400 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val 
                405                 410                 415     


Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met 
            420                 425                 430         


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
        435                 440                 445             


Leu Gly Lys 
    450     


<210>  23
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4P light chain variant 1

<400>  23

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  24
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human IgG1 heavy chain DNA variant 1

<400>  24
caagtgcaac tggtggaatc cggggccgaa gtgaaaaagc ccggagccac tgtgaagatc       60

tcttgcaaag tgtccggcta caccttcacc gactattaca tgcactgggt ccagcaggca      120

cctgggaagg gccttgagtg gatgggtctg gtcgatcccg aggacggcga aactatctac      180

gccgagaagt tccagggtcg cgtcaccatc accgccgaca cttccaccga caccgcgtac      240

atggagctgt ccagcttgag gtccgaggac acagccgtgt actactgcgc cacggatgct      300

cggggaagcg gcagctacta cccgaaccac ttcgactact ggggacaggg cactctcgtg      360

actgtctcga gcgcttctac aaagggcccc tccgtgttcc cgctcgctcc atcatcgaag      420

tctaccagcg gaggcactgc ggctctcggt tgcctcgtga aggactactt cccggagccg      480

gtgaccgtgt cgtggaacag cggagccctg accagcgggg tgcacacctt tccggccgtc      540

ttgcagtcaa gcggccttta ctccctgtca tcagtggtga ctgtcccgtc cagctcattg      600

ggaacccaaa cctacatctg caatgtgaat cacaaaccta gcaacaccaa ggttgacaag      660

aaagtcgagc ccaaatcgtg tgacaagact cacacttgtc cgccgtgccc ggcacccgaa      720

ctgctgggag gtcccagcgt ctttctgttc cctccaaagc cgaaagacac gctgatgatc      780

tcccgcaccc cggaggtcac ttgcgtggtc gtggacgtgt cacatgagga cccagaggtg      840

aagttcaatt ggtacgtgga tggcgtcgaa gtccacaatg ccaaaactaa gcccagagaa      900

gaacagtaca attcgaccta ccgcgtcgtg tccgtgctca cggtgttgca tcaggattgg      960

ctgaacggga aggaatacaa gtgcaaagtg tccaacaagg cgctgccggc accgatcgag     1020

aaaactatct ccaaagcgaa gggacagcct agggaacctc aagtctacac gctgccacca     1080

tcacgggatg aactgactaa gaatcaagtc tcactgactt gtctggtgaa ggggttttac     1140

cctagcgaca ttgccgtgga gtgggaatcc aacggccagc cagagaacaa ctacaagact     1200

acccctccag tgctcgactc ggatggatcg ttcttccttt actcgaagct caccgtggat     1260

aagtcccggt ggcagcaggg aaacgtgttc tcctgctcgg tgatgcatga agccctccat     1320

aaccactata cccaaaagtc gctgtccctg tcgccgggaa ag                        1362


<210>  25
<211>  657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human IgG1 light chain DNA variant 1

<400>  25
gacattgtga tgacccagtc ccccgattcg cttgcggtgt ccctgggaga acgggccacc       60

attaactgca agagctcaca gtccgtcctg tattcatcga acaacaagaa ttacctcgca      120

tggtatcagc agaagcctgg acagcctccc aagctgctca tctactgggc tagcacccgc      180

gaatccgggg tgccggatag attctccgga tcgggttcgg gcactgactt cactctgact      240

atcaactcac tgcaagccga ggatgtcgcg gtgtacttct gtcagcagta ctacgacacc      300

ccgacctttg gacaaggcac cagactggag attaagcgta cggtggccgc tccctccgtg      360

ttcatcttcc caccctccga cgagcagctg aagtccggca ccgcctccgt cgtgtgcctg      420

ctgaacaact tctacccccg cgaggccaag gtgcagtgga aggtggacaa cgccctgcag      480

tccggcaact cccaggaatc cgtcaccgag caggactcca aggacagcac ctactccctg      540

tcctccaccc tgaccctgtc caaggccgac tacgagaagc acaaggtgta cgcctgcgaa      600

gtgacccacc agggcctgtc cagccccgtg accaagtcct tcaaccgggg cgagtgc         657


<210>  26
<211>  1353
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human IgG4P heavy chain DNA variant 1

<400>  26
caagtgcaac tggtggaatc cggggccgaa gtgaaaaagc ccggagccac tgtgaagatc       60

tcttgcaaag tgtccggcta caccttcacc gactattaca tgcactgggt ccagcaggca      120

cctgggaagg gccttgagtg gatgggtctg gtcgatcccg aggacggcga aactatctac      180

gccgagaagt tccagggtcg cgtcaccatc accgccgaca cttccaccga caccgcgtac      240

atggagctgt ccagcttgag gtccgaggac acagccgtgt actactgcgc cacggatgct      300

cggggaagcg gcagctacta cccgaaccac ttcgactact ggggacaggg cactctcgtg      360

actgtctcga gcgcttctac aaagggcccc tccgtgttcc ctctggcccc ttgctcccgg      420

tccacctccg agtctaccgc cgctctgggc tgcctggtca aggactactt ccccgagccc      480

gtgacagtgt cctggaactc tggcgccctg acctccggcg tgcacacctt ccctgccgtg      540

ctgcagtcct ccggcctgta ctccctgtcc tccgtcgtga ccgtgccctc ctccagcctg      600

ggcaccaaga cctacacctg taacgtggac cacaagccct ccaacaccaa ggtggacaag      660

cgggtggaat ctaagtacgg ccctccctgc cccccctgcc ctgcccctga atttctgggc      720

ggaccttccg tgttcctgtt ccccccaaag cccaaggaca ccctgatgat ctcccggacc      780

cccgaagtga cctgcgtggt ggtggacgtg tcccaggaag atcccgaggt ccagttcaat      840

tggtacgtgg acggcgtgga agtgcacaat gccaagacca agcccagaga ggaacagttc      900

aactccacct accgggtggt gtccgtgctg accgtgctgc accaggactg gctgaacggc      960

aaagagtaca agtgcaaggt gtccaacaag ggcctgccct ccagcatcga aaagaccatc     1020

tccaaggcca agggccagcc ccgcgagccc caggtgtaca ccctgccccc tagccaggaa     1080

gagatgacca agaaccaggt gtccctgacc tgtctggtca agggcttcta cccctccgac     1140

attgccgtgg aatgggagtc caacggccag cccgagaaca actacaagac caccccccct     1200

gtgctggaca gcgacggctc cttcttcctg tactctcggc tgaccgtgga caagtcccgg     1260

tggcaggaag gcaacgtctt ctcctgctcc gtgatgcacg aggccctgca caaccactac     1320

acccagaagt ccctgtccct gagcctgggc aag                                  1353


<210>  27
<211>  657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human IgG4P light chain DNA variant 1

<400>  27
gacattgtga tgacccagtc ccccgattcg cttgcggtgt ccctgggaga acgggccacc       60

attaactgca agagctcaca gtccgtcctg tattcatcga acaacaagaa ttacctcgca      120

tggtatcagc agaagcctgg acagcctccc aagctgctca tctactgggc tagcacccgc      180

gaatccgggg tgccggatag attctccgga tcgggttcgg gcactgactt cactctgact      240

atcaactcac tgcaagccga ggatgtcgcg gtgtacttct gtcagcagta ctacgacacc      300

ccgacctttg gacaaggcac cagactggag attaagcgta cggtggccgc tccctccgtg      360

ttcatcttcc caccctccga cgagcagctg aagtccggca ccgcctccgt cgtgtgcctg      420

ctgaacaact tctacccccg cgaggccaag gtgcagtgga aggtggacaa cgccctgcag      480

tccggcaact cccaggaatc cgtcaccgag caggactcca aggacagcac ctactccctg      540

tcctccaccc tgaccctgtc caaggccgac tacgagaagc acaaggtgta cgcctgcgaa      600

gtgacccacc agggcctgtc cagccccgtg accaagtcct tcaaccgggg cgagtgc         657


<210>  28
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse full length IgG1 heavy chain variant 2

<400>  28

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Lys Thr Thr 
        115                 120                 125             


Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala Ala Gln Thr Asn 
    130                 135                 140                 


Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu Ser Ser Ser Val 
            180                 185                 190         


Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr Val Thr Cys Asn Val 
        195                 200                 205             


Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg 
    210                 215                 220                 


Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val Pro Glu Val Ser Ser 
225                 230                 235                 240 


Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val Leu Thr Ile Thr Leu 
                245                 250                 255     


Thr Pro Lys Val Thr Cys Val Val Val Asp Ile Ser Lys Asp Asp Pro 
            260                 265                 270         


Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala 
        275                 280                 285             


Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Ser Val 
    290                 295                 300                 


Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu Asn Gly Lys Glu Phe 
305                 310                 315                 320 


Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala Pro Ile Glu Lys Thr 
                325                 330                 335     


Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln Val Tyr Thr Ile 
            340                 345                 350         


Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys Val Ser Leu Thr Cys 
        355                 360                 365             


Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr Val Glu Trp Gln Trp 
    370                 375                 380                 


Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln Pro Ile Met Asp 
385                 390                 395                 400 


Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn Val Gln Lys Ser 
                405                 410                 415     


Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly 
            420                 425                 430         


Leu His Asn His His Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
        435                 440                 445             


<210>  29
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse full length IgG1 light chain variant 2

<400>  29

Asp Ile Val Met Thr Gln Thr Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
        115                 120                 125             


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
145                 150                 155                 160 


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
            180                 185                 190         


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
        195                 200                 205             


Pro Ile Val Lys Ser Phe Asn Arg Asn Glu Cys 
    210                 215                 


<210>  30
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human full length IgG1 heavy chain variant 1

<400>  30

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120                 125             


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
    130                 135                 140                 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
            180                 185                 190         


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
        195                 200                 205             


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
    210                 215                 220                 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
225                 230                 235                 240 


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
                245                 250                 255     


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
            260                 265                 270         


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
        275                 280                 285             


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
    290                 295                 300                 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
305                 310                 315                 320 


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
                325                 330                 335     


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
            340                 345                 350         


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
        355                 360                 365             


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
    370                 375                 380                 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
385                 390                 395                 400 


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
                405                 410                 415     


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
            420                 425                 430         


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
        435                 440                 445             


Ser Leu Ser Pro Gly Lys 
    450                 


<210>  31
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human full length IgG1 light chain variant 1

<400>  31

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  32
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fab heavy chain variant 2

<400>  32

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120                 125             


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
    130                 135                 140                 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
            180                 185                 190         


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
        195                 200                 205             


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
    210                 215                 220                 


Lys Ser Cys 
225         


<210>  33
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fab light chain variant 2

<400>  33

Asp Ile Val Met Thr Gln Thr Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  34
<211>  451
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4P heavy chain variant 2

<400>  34

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met His Trp Val Gln Gln Ala Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Leu Val Asp Pro Glu Asp Gly Glu Thr Ile Tyr Ala Glu Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Ile Thr Ala Asp Thr Ser Thr Asp Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asp Ala Arg Gly Ser Gly Ser Tyr Tyr Pro Asn His Phe Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
        115                 120                 125             


Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu 
    130                 135                 140                 


Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
145                 150                 155                 160 


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
                165                 170                 175     


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
            180                 185                 190         


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn 
        195                 200                 205             


Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Ser 
    210                 215                 220                 


Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly 
225                 230                 235                 240 


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
                245                 250                 255     


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
            260                 265                 270         


Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
        275                 280                 285             


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr 
    290                 295                 300                 


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
305                 310                 315                 320 


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile 
                325                 330                 335     


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
            340                 345                 350         


Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser 
        355                 360                 365             


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
    370                 375                 380                 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
385                 390                 395                 400 


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val 
                405                 410                 415     


Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met 
            420                 425                 430         


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
        435                 440                 445             


Leu Gly Lys 
    450     


<210>  35
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4P light chain variant 2

<400>  35

Asp Ile Val Met Thr Gln Thr Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val Leu Tyr Ser 
            20                  25                  30          


Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Asn Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln 
                85                  90                  95      


Tyr Tyr Asp Thr Pro Thr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  36
<211>  16654
<212>  DNA
<213>  Homo sapiens

<400>  36
actcggtgcg ccttccgcgg accgggcgac ccagtgcacg gccgccgcgt cactctcggt       60

cccgctgacc ccgcgccgag ccccggcggc tctggccgcg gccgcactca gcgccacgcg      120

tcgaaagcgc aggccccgag gacccgccgc actgacaggt gagcgcggac gcacccggca      180

gggatgtgag tgggcggagg gaagagggcc gcaaaccaac ccaggacccg ctcagttcca      240

cgcgcggcag ccctccgtgc gcgcaggctc gggtgcgttg ttcgcggggg tgaattgtga      300

agaaccatcg cggggtcctt cctgctgagg ccgcggacac cgtgacctcg ctgctctggg      360

tctgcaggga aacgtaggaa aaaaagttgt caggagcggg caggatgacc cccacatccc      420

gtttccacct cccggaggcc cccgaacacg ctcctggtgc tggtggcagc agcgcctggc      480

agacgcgccc gcttagcgag ggcgcgaagt ccaggccgcc agagcgcagg agcatccgga      540

cctgctagtc ggccgctgac tgcgcggcga gttgccttga gagggtccca tgtgcttggg      600

gcgccgcgct gggtctgggg gcgtcttggg gcgcccattg gagtccgcgg gttggagcat      660

ccggagaatc catgatgtgt gcatttgccg atccccgagg tgagatggag actggcaagg      720

gcagagccgc tgtgttcagc cacagcggaa aaccgaacgg tgggtaatcc gacagctgcg      780

gtgcggggcg cggccctggc cgcggggtcc agcgaacccg cagtgctcac aaggcagaca      840

ccacacgcgc tcgcggaccg gccacgcact cgcgggcgct cgcttctcta ctccagcctc      900

ttccccgccc cgcgcacgcc cgagctgaat ggtagacgtt ctggcgccgg gcagcggcca      960

ccggctggtt cccacttccg cgcgcacccc ttaaactgtg ttctagaggc cccagcctcg     1020

ccttgcagcg cctcactagc tcctgaggac tagggactgg cggctgaggc gggttggcgg     1080

ctgcaacgag ctgggcgtct ttcgttctct ctcgctgcct ggctggctcc gctggcccct     1140

ccacagcttg cggagcaagg ccatagcagg ggagtgggag gtatattggg gctgtcacct     1200

ccttgctggc cggagttatt tgtagactac agactccgga agaacagacg cgccaccgct     1260

ctcgcttggc attgccttcg gatcgcagct cctccttggg ggtgccccag cttggcgttt     1320

atttgcctgc gccaggctct ggcgacggtc accgggccag gcggggaggg acggacggca     1380

ggtgaccagc ctctgctgtg aagaaattcc tgcgcgcccg gagctgtccc taatgcattc     1440

ccgggtcgaa tccgtctact gccttcccct cctcgaccga ctccgaatct cggctcttat     1500

agacagaaat acagcctcag cgttaggggt taaaatcccc ctcttaaacg gtccgagggc     1560

agagaggtga ccaccgatag gtaattggat ctcctgctgg aaagagcaaa tctgagcggt     1620

gtgcgcgtct gtttatgttc cccttcgaga tggtgccagg acacgaactg attaaaacaa     1680

tctattgtgt taagtgggtc actagggttt taagctgtcc cagggacccc agagtagtgg     1740

cttccttctg gctgtacaca caagttaaat aaatagcgta gaagaggtta agataacccc     1800

attctagggt gaggagtcct ctttcatccc tagggcttcc ccctcccctt ttctcttttt     1860

ttggaaggag ggggagcatg agagtcttga gggggggatg tacttttcaa agcaaggagg     1920

gaaagatctt aagaaaacta tatattctca ctgcccccca agccaagtct ataacagtag     1980

gtgatttgat tactatctct ggataaatgg cactgtcaaa ttgttaatat taactatttc     2040

agggattttt agcagggtag tggcagtatg tgtgcgtgtg tgtgtgtgtg tctgtgtgtg     2100

tgtgtttaac ctccaggtca ttgtaggaat tagagtcttt tgtaaacttt gtaatttcac     2160

aggtttccta ttttcttaaa agttcatttt tagtgaaatg ttttggtaac ccacgctctg     2220

taggaaatcc aggttggcta atgcggtctt tatgtgagta gttacacagg gaaggataaa     2280

aaccttttat gtcctacatc tctgaatgag ggctgcctac cctgtctttg aaactaagcc     2340

gaagatgcct tcagtctgaa tggtcaagta ttaaaagtga taaaatgcaa agaaatttca     2400

tgccgcagac acctccccca agaactgctt gttgacagca aagctgtgga acatgttcca     2460

caacagagag taaaggacag ccaggaaata taaacctttt atgtaaagga aaggcaggtg     2520

ggggacagtg gttaggggag gtgactgcag cctctaacca aaaggcaacc atcaggcaag     2580

tgctaccagc ccgtgtcttc gatctgcaag gaattttctt tagttttaac atatgctctt     2640

agaaattcaa agtacaacag gaattcctgg gacaagagaa atctttttat tcacatgtga     2700

acatgaagat acaaaataga taattatttt atttatagca ctcttcaaat tgtattgcat     2760

tagaaaacat atccattgac ccactgttaa ggacagcact gggtgtcaat aggacagtgg     2820

ttaaggacct gtgtttgggg ctagatagaa ttgggtttaa actgctggct gggctgggca     2880

cagtggctca caccggtaat cccagcactt tgggaggcca aggaggacgg atcacctgag     2940

gtcgggagtt cgacaccagc ctgaccaaca tggaaaaatc ccgtctctac taaaaacaca     3000

aaattagcca ggcatggtgg tgcatgcctg caatcccagc tacttgggag gctgaggcag     3060

gagaattgct tgaaaccggg aggcggaggt tgtggtgagc ccagatagcg ccattgcatt     3120

ccagcctggg caacacgagt gaaaactccg tcaaaaaaac aaaacaaaac aaacaaacaa     3180

aaaaatgctg gctttcccac ttatgagctg tgtgaccttg gacaaatttc caacttttct     3240

gagtgtagat tccctgattg gtaaaaggaa gatgatatta tctacctcat attttgttat     3300

gaaaaataaa tgataaaatt gggtcagaaa tcagcataat gcctggcaca gtaagggctt     3360

caaaataaaa ggtagctctt attattagta atggtgttag gaaaagtagc aatgttatac     3420

agaaccagga tatatcacag ggcagttctg aaattaaatc ctgaatcctg gccgggtgag     3480

gtggctcacg cttgtaatcc taagcacttt cggagactga ggcaggcgga tcacgaggtc     3540

aggagtttga gaccagcctg gccaacacac tgaaatcccg tctctactaa aaatacaaaa     3600

attagctggg tgtggtggca ggcgcctata atctcagcta cttgggaggc tgaggcagga     3660

gaatcacttg agcccaggag gcggaggttg cagtgagctg atatcgtgcc agtgcactcc     3720

agcctgggtg acatctctta aaaaaaaaaa tcctgaatac cacactacgc agtgactaac     3780

acatctttca ctacagaaca gaacctgtaa cttggccgtc tctcagcagt gctgctcagt     3840

gaacatttaa taatttatta ctttctaact cgtttcttgt tgacctcaag aattgtacat     3900

agtcattaac tttcctaaga aaatctttga caaacataga gctcctgaga tatttcacaa     3960

ccaggtggtc tcctccctgt cctatgcaat gttgggcccc agcctgattt agccgacctg     4020

gtcttcagac ttgagaggct gtttagggtt cttacaacac aaaggggatg agactttatc     4080

ctctacctgt gtgccaacaa gggattcttc ttatctcctt ggtgcaactt gtctgaaaaa     4140

gaaagtcaac acaattatct tcttaaaagt taaagatcaa attaaaaata agctatagtt     4200

ttcccaaaga tttagacctg agaaaaagga atagatcttt ctaaaacctg gcctgcactt     4260

agagcatttg tagtcacttc cactatttct tatgctgaga gaataatttg atgtcatgcc     4320

tattgaatgt ctttctaaag cttgattcat caggaggaac tgaccagaag tccatgcaca     4380

gacatttggc tttcactgta attcctactc aaaactccct ttcactgatt atgagcaggt     4440

tgcttagcta aaatgagaaa tacagcaaga agagggatgg aaagagctgg cttaaacttt     4500

cccaaagaca tcatgaacac tgggaacagg tcatacatat accattttta tttctcagtc     4560

ctcagtatta gaatattgtg ttcctagagg ctttgtgaaa attggaatac attgccatga     4620

cctgcacagg atagaggtgg ttaatatggc ttaccttgct tgtagatgtt tactcttgat     4680

ccctaaggga aactggaagg aaagcatgct gtagagagga cctgctttga gagtatgtgt     4740

cctctgggac aagtggaatg aaagagagga tagaggcaaa gagaaaaaaa ggtggagatg     4800

gggtgagact tgctggtgga cagttgcaaa gaaaactcat gatgagaata acatcacatt     4860

tcttgaagaa gctgaactgc attgatggga agttgaggca gagtttgaaa agaagatata     4920

ctgtctgcaa aaaagaatca acggagatgt caggtatctt gatgcaccct gagtctatga     4980

gatgcagctt aatttaattt aatgatatga gtaggccatt tcataagtgg tggaaattac     5040

cataaagtgt ttgcagctaa tgacggcgca tgaatgatgt cataggaacc taagtctgga     5100

tgagctatgg attgaatttt tactatggga cacccttcta catgttgctg tggaaaagaa     5160

ttgtcctcaa gaaatgtaca tcttctgcat ttcctctaca tctctgatat ttacaaagtg     5220

cacattattt ggtgatacta caactgggat ttcaagtgca catcactact atttccttca     5280

ctgaagaatc aagagcagtc tgggggtggg gagagcttga gtgattgaca aggatgtggc     5340

agagccctgg ttccactatg aattatagtt cttaccctgc ttactgtcac tcatgtagag     5400

cagctagtgt gcctcagagc actgctgttc tctgagaagc tgaggtctcg attgacattc     5460

ttgaagttgg tgtttacttc tctctgaata aacaaaggtt ggcaactcag acatcttaca     5520

ataagtacta aagatttttg aagaataggt ttttaaaaaa tgaaaaaaca tttcactttc     5580

catagctaat aaatcttatt ttgaggaaaa tgtacttttc tttaaaaaaa aaaaaagcct     5640

gtctgtcact ctagaccctt tggcttagaa ggtaggcaca ctcacataga aacagaaagt     5700

ctgtccaaat taaaactgaa aaccacagtt gactaatttt gaatttatag ctctgctgtt     5760

ggcttctgcg atagtattaa tttcaatggc ttcaattaga aaatgaaacc catagcattc     5820

catatgagaa caggtaaaaa gtcagggaca tttggagttt tccaagaaaa agaaagacaa     5880

gtcttaggaa gctctctagg atggaaggaa tttgccacac tgagagttag acatccaaag     5940

gatagcaatt ggctcttctg ctcatgggca ctggtgaagg cattttaaaa tgcgaagaat     6000

ggtacctctg taaatcaatg aggttcataa taatcatgca tttaccaaat ttttataagc     6060

acctgccttg tgccaggcac tgagggtaag gtgatgaata agccctcatc aactgtcaga     6120

ctagatattt actcaataac agatgtgaaa atgccaagaa ggaaaagttg agtataaaga     6180

aagccttaag ttggttcaga gaaataaaat tgcatttttc ggatagatgt ttatggaatc     6240

ggccttatga ggtaaacttg tcctatgcag tgaacataca ttcccagtta gtctcagatt     6300

ggcctctgtg atgacaaact cagagggtcc tggtctagga ggggtgaatt tgtctggagg     6360

ccattttcag gaggtatgga ggaagactgg ggcataggcc tggggccatc ccatgtactc     6420

ctcctctgaa atggggagca actgaattgt gttttatttt agatcttcgt ccaacttgaa     6480

taccagaaat tcgtgaaacc ttctcaaatt cacactatat tttgagacca ggagaaggct     6540

ccttgagaaa ttgccacact gtcttatcct agtctctgga aaaattcagt cctgtattat     6600

aactgggcgt ttctcataag tgcttttttt ttttcttttt ctttttataa tactttaagt     6660

tctgggatac atgtgcagaa tgtgcaggtt tgttacatag gtatacacgt gccatggtgg     6720

tttgctgcac ccatcaaccc atcatctaca ttaggtattt ctcctaatgc taccccttcc     6780

ctagcccccc acccctcgac aggccccagt gtttgatgtt cccctccctg tgtccatgtg     6840

ttctcattgt tcaactccca cttatgagtg agagcatgcg gtgtttggtt ttctgttcct     6900

gtgttagttt gctgagaatg atggtttttg gcttcatcca cgtccctgca aaggacatga     6960

actcatcctt tttatggctg catagtattc catggtgtat atgtgccaca tttgctttat     7020

ccagtctatc attgatgagc acttgggttg gctccaagtc tttgctatta tgactagtac     7080

tgcgataaac atgtgtatat gtgtctttat attagaatga tttataatcc tttaggtcta     7140

tacccagtaa taggattcct gggtcaaatg gtatttctgg ttctagatcc ttgaggaatt     7200

gccacactgt cttccacaac ggttgaacta atttacactc ccaccaacag tgtaaaagca     7260

ttcctatttc tccacatcct ctccaacatc tgttgtttcc tgactcttta atgacggcca     7320

ttctaactgg cgtgaaatgg tatctcattg tggttttgat ttgcatttct ctaatgacca     7380

ccgatgatga gctttttctc atatgtttgc tggccccata aatgtcttct tttgagaaat     7440

gtctgttcat atcctttgcc cactttttga tggggttgtt tgcttttttc ttgtaaattt     7500

gtttgagttc ctggtagatt ctggatatta gccctttgtc agatggatag actgcaaaaa     7560

ttttctccca ttctgtaggt tgcctgttca ctctgatggt agtttctttt gccatgcaga     7620

agctgtttag tttaattaga tccaatttgt cagttttggc ttttgttgcc attgcttttg     7680

gtgttttagt catgaagtct ttgcccatgc ctatgtcctg aattgtattg ccctggtttt     7740

cttctaggat ttttatggtt ttaggtctta catttaagtc tttaatccat cttgaattaa     7800

tttttgtata aggtgtaagg aaagggtcca gtttcagttt tctgcgtatg gctagccagt     7860

tttcccaacg ttatttatta aatagggaat cctttcccca ttgcttgttt ttgtcaggtt     7920

tgtcaaagat cagacggttg tagatgtgtg gtgttatttc tgaggcctct gttctgttcc     7980

attggtctgt atatctgctt tgataccagt accgtgctgt tttggttact gtagccttgt     8040

agtacagttt gaagtcaggt agcgtgatgc tttgttcttt ttgcttagga ttgtcttggc     8100

tatatgggct cttttttggt tctatatgaa atttaaagta gttttttcat agacatctat     8160

agaactctcc accccaaatc aacagaatat acattcttct cagtatcgca tcacacttat     8220

tctaaaatga ccacataatt agaagtaaaa cactcctcag caaatgcaaa aaacagaaat     8280

cctaacagtc tgtcagaccc cagtgcaatc aaattagaac tcaggattaa gaaacttact     8340

gaaaaccaca caactgcatg gaaactgaac aacctgctcc tgaatgacta ctgggtaaat     8400

aacaaaatta aggcagaaat aaataggttc tttgaaagaa atgagaacaa agacacaata     8460

taccagaatc tctgggacac agctaaagca gtgtttagag ggaaattgat agcactaaat     8520

gcccacagga gaaagcggga cagatctaaa atcgacaccc taatatcaca attaaaagaa     8580

ctagagaaac aagagcaaac aaattcaaaa gctagcagaa gacaagaaat aactaagatc     8640

agagcagaac tgaaggagat agagacacaa aaaacccttc aaaaaatcaa tgaatccagg     8700

agctgttttt ttttttttga aaagattaat gaaatagact gctagccaaa ctaataaagg     8760

agaaaagaga gaagaatcaa atagacaata gacacaataa aaagtgataa aggggatatc     8820

accactgatc ccacagaaat acaaactacc atcagagaat actataaaca cctctacgca     8880

aataaactag aaaattggga agaaatggat acattcctga acgcatacac cctcccaaga     8940

ctaaaccagg aagaagttga atccctgaat agaccaatag caagttctga aattgaggca     9000

gtaattagta gcctaccaac caaaaaaagc ccaggaccag acagattcac agccgaagtc     9060

taccagaggt acaaagagga gctagtacca ttccttctga aactattcca aacaatagaa     9120

aaagaaggac tcctccctaa ctcattttat gaggccagca tcatcctgat accaaaacct     9180

ggcagagaca caacataaag gaaattcagg ccaatatccc tgatgaacat tgatgtgaaa     9240

atcctcaata aaatactggc agcacatcaa aaagcttacc tgccatgatc aagttggctt     9300

catccctggg atgcaaggct ggttcaacat acgcaaatca ataaacgtaa tcaatcacat     9360

acacagaacc aatgacaaaa accacatgat tatctcaata gatgcagaaa aggccttcga     9420

taaaattcaa caccccttca atgctaagaa ctctcaataa actaggtatt gatgaaacat     9480

atctcaaaat aataagagtt atttatgaca aacccacagc cagtatcata ctgaatgggc     9540

aaaagctgga agcattccct ttgaaaaccg ccacaaggat gacttctctc accactccta     9600

ttcaacatac tattggaagt tctggccagg gcaagcaggc aagacaaata aataaagggt     9660

attcaaatag gaagagagga agtcaaattg tctctgtttg cagatgacat gattgtatat     9720

ttagaaaacc ccatcatctc agcccaaaat ctccttaagc tgataagcaa cttcagcaaa     9780

gtctcaggat acaaaatcaa tgtgcaaaaa tcacaagcat tcctatacac caataataga     9840

caaacagcca aatcatgagt gaactcccat tcacaagtgc tacaaagaat aaaataccta     9900

ggaatccaac ttacaaggga tgtgaaggac ctcttcaagg agaactacag accactgctc     9960

aaggtaataa gagaggacac aaacaaatgg aaaaacattc catgctcatg gataggaaga    10020

atcaatatta tgaaaatggc catactgccc aaaacaattt atagattcaa tgccatcccc    10080

atcaagctac cactgacttc cttcacagaa ttagaaaaaa actactttaa atttcatatg    10140

gaactgaaaa aagagccggt atagccaaga cgatcctaag caaaaagaaa aaagctggaa    10200

gcatcatgct acctgacttc aaactatact acaaggctac agtaacaaaa acagcatggt    10260

atagtataag tgcttttttt taaaagacaa agtaaagtaa tttttttgtt gttggggtaa    10320

aacaaaagct ctgcataaag agcagggatg ttgtaacata cactgaccaa aggtgggaaa    10380

cctacagttg gagcagaagc tgaatgtcac attatcagct ccgaacttat aatggtctaa    10440

aagtactagg ttaatgttgg aaagatggtg ccatttaaag atatcttaaa ttcaatattt    10500

aattatttta atttgacatt atcctagagt tgaatggtgt ttacttacca tgtgctgttc    10560

attagaaaat ctagatccta cactgccttt gcgcaaggta gttgctctaa taataccaac    10620

ctgtccagtt ttggtgggag aaataatgtt actgttaagt tgcacttagt ggttattatg    10680

tgtaatactg gtattccaaa gagagaagga aaatgtttgc tacacagctg tgttcttagg    10740

ttcagaaaac cacaggagtg ggacaggaga accttcagga ttcaggtccg attgttgtga    10800

tggccgcagg agggagactg tgaatttgaa actgcatcca ttgaaaagaa aatccctcca    10860

cactttaata attctctccg tgccccatgg cagcaagtgc ttataggcct tgctactcaa    10920

agcttaataa aaaggcagac ctgctgaatc ataatctgca tcttaacaat atccccagat    10980

attgtctgca ctttcttttt ttttttcttt ctttctttct tctttttttt gagacagagt    11040

ctcgctcagt cacccaggct ggagtgcagt ggcgcgatct cggctcactg caagctctga    11100

ctcccgggtt cacgccattc tcctgcctta gactcccgag tagctggcac tacaggcgcc    11160

cgccactacg cccggctaat ttttttgtat ttttagtaga gatggggttt caccgtgtta    11220

gccaggatgg tctcgatctc ctgacctcgt gatccgccgc ccatctgggc ctcccaaagt    11280

gctgggatta caggcgtgag ccactgcgcc cggccaattg tctgcacttt caagtctaag    11340

aagcactgtc ccaggaggaa aatcttttta gtctgaggct tctctcttgc actctccttt    11400

ttaaaaatat gctgctcctt ctccaccttt ccctcttctt ccgccttttc tgtctgcctt    11460

tactacctcc cctgaacatt caacttgtag aagagttccc ctttctctga attgcattct    11520

tcacttcatt cattcttttc tctctctgtc tatggtttcc tattttttgt cggttttccc    11580

tcacctcacc tccttgttat tttttgccat tgttcacata tcactgccct ttcagaccca    11640

tatctagctt ctgacccatc cactaatcca ttgccactaa tttattcagc atgcccatgc    11700

catttattaa tgtaaatatt tgcacatact tgttctatca tgcccatttt tctacctttt    11760

aaattgtata tacacagaca catgtgaatg acatatttca ctatttaaaa ggtagaaaaa    11820

tgtatatcgt ggtacaaagt gatacattga gtatctgtgc ccagtttcaa gatgagaata    11880

agtgagaggt gaagcctcat gagtcacgtc acctacacgc tactgtcttt cattccatct    11940

gcagtactgc cacacatttg gttagttctc caattgctgt cacattgaca tcgagttgga    12000

tctgaacagc gtacctgggg agacgaagta ttggtatctt tgcttaaatg gagtgattta    12060

ctgagagaca aagtgacatc tttaagatca gatagcaagt tactgcacca ggagcgcgac    12120

ctttcctgtt ttgttgtttt ccctctgtca aatgccttct ggtctccatg aatctctcct    12180

tccacatatg ataaaaatgc aaagtctcat aagcattcac agctcaggaa gcctcaggct    12240

agttggggag aaaagactgg gaggtttccg gaggaatgaa gtcctctgag cagagaggtt    12300

aattcatctt gctgtaaaac aaaatagaaa ataagttccc ctcaataagt gaacgtaata    12360

caaagacaaa tgtggttggt gccagaggcc aggaagaagt tcttgtgaga ataggtgcag    12420

agaagagcta ggccctggct gagtttaaac cttgacttag tcactgtggg actctgggtg    12480

agttactcca tggattgatg gctgggtcat ggagataata gtacctaatt catacaggta    12540

ctgtgagaag taaatggaat atttcacgtt aagtgtttaa cggtgcgttt aaatgctagg    12600

tgctattatt attaattttt aaattaactt tgccatgttt tgtgtcttcc cctctctgtg    12660

cttcctttct ttagtatgag ccgcacagcc tacacggtgg gagccctgct tctcctcttg    12720

gggaccctgc tgccggctgc tgaagggaaa aagaaagggt cccaaggtgc catccccccg    12780

ccagacaagg cccagcacaa tgactcagag cagactcagt cgccccagca gcctggctcc    12840

aggaaccggg ggcggggcca agggcggggc actgccatgc ccggggagga ggtgctggag    12900

tccagccaag aggccctgca tgtgacggag cgcaaatacc tgaagcgaga ctggtgcaaa    12960

acccagccgc ttaagcagac catccacgag gaaggctgca acagtcgcac catcatcaac    13020

cgcttctgtt acggccagtg caactctttc tacatcccca ggcacatccg gaaggaggaa    13080

ggttcctttc agtcctgctc cttctgcaag cccaagaaat tcactaccat gatggtcaca    13140

ctcaactgcc ctgaactaca gccacctacc aagaagaaga gagtcacacg tgtgaagcag    13200

tgtcgttgca tatccatcga tttggattaa gccaaatcca ggtgcaccca gcatgtccta    13260

ggaatgcagc cccaggaagt cccagaccta aaacaaccag attcttactt ggcttaaacc    13320

tagaggccag aagaaccccc agctgcctcc tggcaggagc ctgcttgtgc gtagttcgtg    13380

tgcatgagtg tggatgggtg cctgtgggtg tttttagaca ccagagaaaa cacagtctct    13440

gctagagagc actccctatt ttgtaaacat atctgcttta atggggatgt accagaaacc    13500

cacctcaccc cggctcacat ctaaaggggc ggggccgtgg tctggttctg actttgtgtt    13560

tttgtgccct cctggggacc agaatctcct ttcggaatga atgttcatgg aagaggctcc    13620

tctgagggca agagacctgt tttagtgctg cattcgacat ggaaaagtcc ttttaacctg    13680

tgcttgcatc ctcctttcct cctcctcctc acaatccatc tcttcttaag ttgatagtga    13740

ctatgtcagt ctaatctctt gtttgccaag gttcctaaat taattcactt aaccatgatg    13800

caaatgtttt tcattttgtg aagaccctcc agactctggg agaggctggt gtgggcaagg    13860

acaagcagga tagtggagtg agaaagggag ggtggagggt gaggccaaat caggtccagc    13920

aaaagtcagt agggacattg cagaagcttg aaaggccaat accagaacac aggctgatgc    13980

ttctgagaaa gtcttttcct agtatttaac agaacccaag tgaacagagg agaaatgaga    14040

ttgccagaaa gtgattaact ttggccgttg caatctgctc aaacctaaca ccaaactgaa    14100

aacataaata ctgaccactc ctatgttcgg acccaagcaa gttagctaaa ccaaaccaac    14160

tcctctgctt tgtccctcag gtggaaaaga gaggtagttt agaactctct gcataggggt    14220

gggaattaat caaaaaccgc agaggctgaa attcctaata cctttccttt atcgtggtta    14280

tagtcagctc atttccattc cactatttcc cataatgctt ctgagagcca ctaacttgat    14340

tgataaagat cctgcctctg ctgagtgtac ctgacagtag tctaagatga gagagtttag    14400

ggactactct gttttagcaa gagatatttt gggggtcttt ttgttttaac tattgtcagg    14460

agattgggct aaagagaaga cgacgagagt aaggaaataa agggaattgc ctctggctag    14520

agagtagtta ggtgttaata cctggtagag atgtaaggga tatgacctcc ctttctttat    14580

gtgctcactg aggatctgag gggaccctgt taggagagca tagcatcatg atgtattagc    14640

tgttcatctg ctactggttg gatggacata actattgtaa ctattcagta tttactggta    14700

ggcactgtcc tctgattaaa cttggcctac tggcaatggc tacttaggat tgatctaagg    14760

gccaaagtgc agggtgggtg aactttattg tactttggat ttggttaacc tgttttcttc    14820

aagcctgagg ttttatatac aaactccctg aatactcttt ttgccttgta tcttctcagc    14880

ctcctagcca agtcctatgt aatatggaaa acaaacactg cagacttgag attcagttgc    14940

cgatcaaggc tctggcattc agagaaccct tgcaactcga gaagctgttt ttatttcgtt    15000

tttgttttga tccagtgctc tcccatctaa caactaaaca ggagccattt caaggcggga    15060

gatattttaa acacccaaaa tgttgggtct gattttcaaa cttttaaact cactactgat    15120

gattctcacg ctaggcgaat ttgtccaaac acatagtgtg tgtgttttgt atacactgta    15180

tgaccccacc ccaaatcttt gtattgtcca cattctccaa caataaagca cagagtggat    15240

ttaattaagc acacaaatgc taaggcagaa ttttgagggt gggagagaag aaaagggaaa    15300

gaagctgaaa atgtaaaacc acaccaggga ggaaaaatga cattcagaac cagcaaacac    15360

tgaatttctc ttgttgtttt aactctgcca caagaatgca atttcgttaa cggagatgac    15420

ttaagttggc agcagtaatc ttcttttagg agcttgtacc acagtcttgc acataagtgc    15480

agatttggct caagtaaaga gaatttcctc aacactaact tcactgggat aatcagcagc    15540

gtaactaccc taaaagcata tcactagcca aagagggaaa tatctgttct tcttactgtg    15600

cctatattaa gactagtaca aatgtggtgt gtcttccaac tttcattgaa aatgccatat    15660

ctataccata ttttattcga gtcactgatg atgtaatgat atattttttc attattatag    15720

tagaatattt ttatggcaag atatttgtgg tcttgatcat acctattaaa ataatgccaa    15780

acaccaaata tgaattttat gatgtacact ttgtgcttgg cattaaaaga aaaaaacaca    15840

catcctggaa gtctgtaagt tgttttttgt tactgtaggt cttcaaagtt aagagtgtaa    15900

gtgaaaaatc tggaggagag gataatttcc actgtgtgga atgtgaatag ttaaatgaaa    15960

agttatggtt atttaatgta attattactt caaatccttt ggtcactgtg atttcaagca    16020

tgttttcttt ttctccttta tatgactttc tctgagttgg gcaaagaaga agctgacaca    16080

ccgtatgttg ttagagtctt ttatctggtc aggggaaaca aaatcttgac ccagctgaac    16140

atgtcttcct gagtcagtgc ctgaatcttt attttttaaa ttgaatgttc cttaaaggtt    16200

aacatttcta aagcaatatt aagaaagact ttaaatgtta ttttggaaga cttacgatgc    16260

atgtatacaa acgaatagca gataatgatg actagttcac acataaagtc cttttaagga    16320

gaaaatctaa aatgaaaagt ggataaacag aacatttata agtgatcagt taatgcctaa    16380

gagtgaaagt agttctattg acattcctca agatatttaa tatcaactgc attatgtatt    16440

atgtctgctt aaatcattta aaaacggcaa agaattatat agactatgag gtaccttgct    16500

gtgtaggagg atgaaagggg agttgatagt ctcataaaac taatttggct tcaagtttca    16560

tgaatctgta actagaattt aattttcacc ccaataatgt tctatatagc ctttgctaaa    16620

gagcaactaa taaattaaac ctattctttc tgtg                                16654


<210>  37
<211>  555
<212>  DNA
<213>  Homo sapiens

<400>  37
atgagccgca cagcctacac ggtgggagcc ctgcttctcc tcttggggac cctgctgccg       60

gctgctgaag ggaaaaagaa agggtcccaa ggtgccatcc ccccgccaga caaggcccag      120

cacaatgact cagagcagac tcagtcgccc cagcagcctg gctccaggaa ccgggggcgg      180

ggccaagggc ggggcactgc catgcccggg gaggaggtgc tggagtccag ccaagaggcc      240

ctgcatgtga cggagcgcaa atacctgaag cgagactggt gcaaaaccca gccgcttaag      300

cagaccatcc acgaggaagg ctgcaacagt cgcaccatca tcaaccgctt ctgttacggc      360

cagtgcaact ctttctacat ccccaggcac atccggaagg aggaaggttc ctttcagtcc      420

tgctccttct gcaagcccaa gaaattcact accatgatgg tcacactcaa ctgccctgaa      480

ctacagccac ctaccaagaa gaagagagtc acacgtgtga agcagtgtcg ttgcatatcc      540

atcgatttgg attaa                                                       555


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse Grem1 forward primer

<400>  38
gcgcaagtat ctgaagcgag                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse Grem1 reverse primer

<400>  39
cggttgatga tagtgcggct                                                   20


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human Grem1 forward primer

<400>  40
aggcccagca caatgactca g                                                 21


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human Grem1 reverse primer

<400>  41
gtctcgcttc aggtatttgc g                                                 21


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta-actin forward primer

<400>  42
gatcattgct cctcctgagc                                                   20


<210>  43
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Beta-actin reverse primer

<400>  43
gtcatagtcc gcctagaagc at                                                22


