                         LISTADO DE SECUENCIAS

<110>  Fundacin Instituto de Investigacin Marqus de Valdecilla
 
<120>  VACUNA MULTIVALENTE PARA EL TRATAMIENTO Y PREVENCIN DE LA 
       TUBERCULOSIS, LISTERIOSIS Y NEUMONA

<130>  1633.16

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-22 de la GAPDH de los gneros bacterianos 
       Mycobacterium, Listeria y Streptococcus


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa is T or V

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa is K or R

<220>
<221>  misc_feature
<222>  (16)..(16)
<223>  Xaa is L or N

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Xaa is A or F

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  Xaa is F or Y

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Xaa is R or A

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Xaa is I or L

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  Xaa is Q or L

<400>  1

Met Xaa Val Xaa Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Xaa 
1               5                   10                  15      


Xaa Xaa Arg Xaa Xaa Xaa 
            20          


<210>  2
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-22 de la GAPDH de Listeria monocytogenes

<400>  2

Met Thr Val Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln 
            20          


<210>  3
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-22 de la GAPDH de Mycobacterium tuberculosis

<400>  3

Met Thr Val Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Asn 
1               5                   10                  15      


Phe Tyr Arg Ala Leu Leu 
            20          


<210>  4
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-22 de la GAPDH de Streptococcus pneumoiae

<400>  4

Met Val Val Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln 
            20          


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-15 de la GAPDH de los gneros bacterianos 
       Mycobacterium, Listeria y Streptococcus


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is T or V

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is K or R

<400>  5

Met Xaa Val Xaa Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg 
1               5                   10                  15  


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-15 de la GAPDH de Listeria monocytogenes

<400>  6

Met Thr Val Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg 
1               5                   10                  15  


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-15 de la GAPDH de Mycobacterium tuberculosis

<400>  7

Met Thr Val Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg 
1               5                   10                  15  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-15 de la GAPDH de Streptococcus pneumoiae

<400>  8

Met Val Val Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg 
1               5                   10                  15  


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino cidos 91 a 99 de la proteina listeriolisina O de L. 
       monocytogenes

<400>  9

Gly Tyr Lys Asp Gly Asn Glu Tyr Ile 
1               5                   


<210>  10
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 23-42 de la GAPDH de Listeria monocytogenes

<400>  10

Asn Val Glu Gly Ile Glu Val Val Ala Ile Asn Asp Leu Thr Asp Ala 
1               5                   10                  15      


Lys Met Leu Ala 
            20  


<210>  11
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 314-336 de la GAPDH de Listeria monocytogenes

<400>  11

Asp Asn Glu Met Ser Tyr Thr Ala Gln Leu Val Arg Thr Leu Glu Tyr 
1               5                   10                  15      


Phe Ala Lys Ile Ala Lys 
            20          


<210>  12
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-22 de la GAPDH de Mycobacterium avium

<400>  12

Met Thr Val Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln 
            20          


<210>  13
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-22 de la GAPDH de Mycobacterium bovis

<400>  13

Met Thr Val Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln 
            20          


<210>  14
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-22 de la GAPDH de Mycobacterium leprae

<400>  14

Met Thr Val Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln 
            20          


<210>  15
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-22 de la GAPDH de Streptococcus aureus

<400>  15

Met Ala Val Lys Val Ala Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln 
            20          


<210>  16
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 189-201 de la listeriolisina O de L. monovytogenes

<400>  16

Trp Asn Glu Lys Tyr Ala Gln Ala Tyr Pro Asn Val Ser 
1               5                   10              


<210>  17
<211>  529
<212>  PRT
<213>  Listeria monocytogenes

<400>  17

Met Lys Lys Ile Met Leu Val Phe Ile Thr Leu Ile Leu Val Ser Leu 
1               5                   10                  15      


Pro Ile Ala Gln Gln Thr Glu Ala Lys Asp Ala Ser Ala Phe His Lys 
            20                  25                  30          


Glu Asn Leu Ile Ser Ser Met Ala Pro Pro Ala Ser Pro Pro Ala Ser 
        35                  40                  45              


Pro Lys Thr Pro Ile Glu Lys Lys His Ala Asp Glu Ile Asp Lys Tyr 
    50                  55                  60                  


Ile Gln Gly Leu Asp Tyr Asn Lys Asn Asn Val Leu Val Tyr His Gly 
65                  70                  75                  80  


Asp Ala Val Thr Asn Val Pro Pro Arg Lys Gly Tyr Lys Asp Gly Asn 
                85                  90                  95      


Glu Tyr Ile Val Val Glu Lys Lys Lys Lys Ser Ile Asn Gln Asn Asn 
            100                 105                 110         


Ala Asp Ile Gln Val Val Asn Ala Ile Ser Ser Leu Thr Tyr Pro Gly 
        115                 120                 125             


Ala Leu Val Lys Ala Asn Ser Glu Leu Val Glu Asn Gln Pro Asp Val 
    130                 135                 140                 


Leu Pro Val Lys Arg Asp Ser Leu Thr Leu Ser Ile Asp Leu Pro Gly 
145                 150                 155                 160 


Met Thr Asn Gln Asp Asn Lys Ile Val Val Lys Asn Ala Thr Lys Ser 
                165                 170                 175     


Asn Val Asn Asn Ala Val Asn Thr Leu Val Glu Arg Trp Asn Glu Lys 
            180                 185                 190         


Tyr Ala Gln Ala Tyr Pro Asn Val Ser Ala Lys Ile Asp Tyr Asp Asp 
        195                 200                 205             


Glu Met Ala Tyr Ser Glu Ser Gln Leu Ile Ala Lys Phe Gly Thr Ala 
    210                 215                 220                 


Phe Lys Ala Val Asn Asn Ser Leu Asn Val Asn Phe Gly Ala Ile Ser 
225                 230                 235                 240 


Glu Gly Lys Met Gln Glu Glu Val Ile Ser Phe Lys Gln Ile Tyr Tyr 
                245                 250                 255     


Asn Val Asn Val Asn Glu Pro Thr Arg Pro Ser Arg Phe Phe Gly Lys 
            260                 265                 270         


Ala Val Thr Lys Glu Gln Leu Gln Ala Leu Gly Val Asn Ala Glu Asn 
        275                 280                 285             


Pro Pro Ala Tyr Ile Ser Ser Val Ala Tyr Gly Arg Gln Val Tyr Leu 
    290                 295                 300                 


Lys Leu Ser Thr Asn Ser His Ser Thr Lys Val Lys Ala Ala Phe Asp 
305                 310                 315                 320 


Ala Ala Val Ser Gly Lys Ser Val Ser Gly Asp Val Glu Leu Thr Asn 
                325                 330                 335     


Ile Ile Lys Asn Ser Ser Phe Lys Ala Val Ile Tyr Gly Gly Ser Ala 
            340                 345                 350         


Lys Asp Glu Val Gln Ile Ile Asp Gly Asn Leu Gly Asp Leu Arg Asp 
        355                 360                 365             


Ile Leu Lys Lys Gly Ala Thr Phe Asn Arg Glu Thr Pro Gly Val Pro 
    370                 375                 380                 


Ile Ala Tyr Thr Thr Asn Phe Leu Lys Asp Asn Glu Leu Ala Val Ile 
385                 390                 395                 400 


Lys Asn Asn Ser Glu Tyr Ile Glu Thr Thr Ser Lys Ala Tyr Thr Asp 
                405                 410                 415     


Gly Lys Ile Asn Ile Asp His Ser Gly Gly Tyr Val Ala Gln Phe Asn 
            420                 425                 430         


Ile Ser Trp Asp Glu Ile Asn Tyr Asp Pro Glu Gly Asn Glu Ile Val 
        435                 440                 445             


Gln His Lys Asn Trp Ser Glu Asn Asn Lys Ser Lys Leu Ala His Phe 
    450                 455                 460                 


Thr Ser Ser Ile Tyr Leu Pro Gly Asn Ala Arg Asn Ile Asn Val Tyr 
465                 470                 475                 480 


Ala Lys Glu Cys Thr Gly Leu Ala Trp Glu Trp Trp Arg Thr Val Ile 
                485                 490                 495     


Asp Asp Arg Asn Leu Pro Leu Val Lys Asn Arg Asn Ile Ser Ile Trp 
            500                 505                 510         


Gly Thr Thr Leu Tyr Pro Lys Tyr Ser Asn Ser Val Asp Asn Pro Ile 
        515                 520                 525             


Glu 
    


<210>  18
<211>  336
<212>  PRT
<213>  Listeria monocytogenes

<400>  18

Met Thr Val Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln Asn Val Glu Gly Ile Glu Val Val Ala Ile 
            20                  25                  30          


Asn Asp Leu Thr Asp Ala Lys Met Leu Ala His Leu Leu Lys Tyr Asp 
        35                  40                  45              


Thr Thr Gln Gly Arg Phe Asp Gly Glu Val Glu Val His Asp Gly Phe 
    50                  55                  60                  


Phe Asn Val Asn Gly Lys Glu Val Lys Val Leu Ala Asn Arg Asn Pro 
65                  70                  75                  80  


Glu Glu Leu Pro Trp Gly Asp Leu Gly Val Asp Ile Val Leu Glu Cys 
                85                  90                  95      


Thr Gly Phe Phe Thr Ala Gln Asp Lys Ala Glu Leu His Ile Lys Ala 
            100                 105                 110         


Gly Ala Lys Lys Val Val Ile Ser Ala Pro Ala Thr Gly Asp Met Lys 
        115                 120                 125             


Thr Ile Val Tyr Asn Val Asn His Glu Thr Leu Asp Gly Thr Glu Thr 
    130                 135                 140                 


Val Ile Ser Gly Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro Met Ala 
145                 150                 155                 160 


Lys Val Leu Glu Asp Glu Phe Gly Val Val Glu Gly Leu Met Thr Thr 
                165                 170                 175     


Ile His Ala Tyr Thr Gly Asp Gln Asn Thr Leu Asp Ala Pro His Pro 
            180                 185                 190         


Lys Gly Asp Phe Arg Arg Ala Arg Ala Ala Ala Glu Asn Ile Ile Pro 
        195                 200                 205             


Asn Thr Thr Gly Ala Ala Lys Ala Ile Gly Glu Val Leu Pro Thr Leu 
    210                 215                 220                 


Lys Gly Lys Leu Asp Gly Ala Ala Gln Arg Val Pro Val Pro Thr Gly 
225                 230                 235                 240 


Ser Leu Thr Glu Leu Val Thr Val Leu Asp Lys Lys Val Thr Val Asp 
                245                 250                 255     


Glu Val Asn Ala Ala Met Glu Ala Ala Ser Asp Pro Glu Thr Phe Gly 
            260                 265                 270         


Tyr Thr Ser Asp Gln Val Val Ser Ser Asp Ile Lys Gly Met Thr Phe 
        275                 280                 285             


Gly Ser Leu Phe Asp Glu Thr Gln Thr Lys Val Leu Thr Val Gly Asp 
    290                 295                 300                 


Gln Gln Leu Val Lys Thr Val Ala Trp Tyr Asp Asn Glu Met Ser Tyr 
305                 310                 315                 320 


Thr Ala Gln Leu Val Arg Thr Leu Glu Tyr Phe Ala Lys Ile Ala Lys 
                325                 330                 335     


<210>  19
<211>  339
<212>  PRT
<213>  Mycobacterium tuberculosis

<400>  19

Met Thr Val Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Asn 
1               5                   10                  15      


Phe Tyr Arg Ala Leu Leu Ala Gln Gln Glu Gln Gly Thr Ala Asp Val 
            20                  25                  30          


Glu Val Val Ala Ala Asn Asp Ile Thr Asp Asn Ser Thr Leu Ala His 
        35                  40                  45              


Leu Leu Lys Phe Asp Ser Ile Leu Gly Arg Leu Pro Cys Asp Val Gly 
    50                  55                  60                  


Leu Glu Gly Asp Asp Thr Ile Val Val Gly His Ala Lys Ile Lys Ala 
65                  70                  75                  80  


Leu Ala Val Arg Glu Gly Pro Ala Ala Leu Pro Trp Gly Asp Leu Gly 
                85                  90                  95      


Val Asp Val Val Val Glu Ser Thr Gly Leu Phe Thr Asn Ala Ala Lys 
            100                 105                 110         


Ala Lys Gly His Leu Asp Ala Gly Ala Lys Lys Val Ile Ile Ser Ala 
        115                 120                 125             


Pro Ala Thr Asp Glu Asp Ile Thr Ile Val Leu Gly Val Asn Asp Asp 
    130                 135                 140                 


Lys Tyr Asp Gly Ser Gln Asn Ile Ile Ser Asn Ala Ser Cys Thr Thr 
145                 150                 155                 160 


Asn Cys Leu Ala Pro Leu Ala Lys Val Leu Asp Asp Glu Phe Gly Ile 
                165                 170                 175     


Val Lys Gly Leu Met Thr Thr Ile His Ala Tyr Thr Gln Asp Gln Asn 
            180                 185                 190         


Leu Gln Asp Gly Pro His Lys Asp Leu Arg Arg Ala Arg Ala Ala Ala 
        195                 200                 205             


Leu Asn Ile Val Pro Thr Ser Thr Gly Ala Ala Lys Ala Ile Gly Leu 
    210                 215                 220                 


Val Met Pro Gln Leu Lys Gly Lys Leu Asp Gly Tyr Ala Leu Arg Val 
225                 230                 235                 240 


Pro Ile Pro Thr Gly Ser Val Thr Asp Leu Thr Val Asp Leu Ser Thr 
                245                 250                 255     


Arg Ala Ser Val Asp Glu Ile Asn Ala Ala Phe Lys Ala Ala Ala Glu 
            260                 265                 270         


Gly Arg Leu Lys Gly Ile Leu Lys Tyr Tyr Asp Ala Pro Ile Val Ser 
        275                 280                 285             


Ser Asp Ile Val Thr Asp Pro His Ser Ser Ile Phe Asp Ser Gly Leu 
    290                 295                 300                 


Thr Lys Val Ile Asp Asp Gln Ala Lys Val Val Ser Trp Tyr Asp Asn 
305                 310                 315                 320 


Glu Trp Gly Tyr Ser Asn Arg Leu Val Asp Leu Val Thr Leu Val Gly 
                325                 330                 335     


Lys Ser Leu 
            


<210>  20
<211>  336
<212>  PRT
<213>  Streptococcus pyogenes

<400>  20

Met Val Val Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln Asn Ile Glu Gly Val Glu Val Thr Arg Ile 
            20                  25                  30          


Asn Asp Leu Thr Asp Pro Asn Met Leu Ala His Leu Leu Lys Tyr Asp 
        35                  40                  45              


Thr Thr Gln Gly Arg Phe Asp Gly Thr Val Glu Val Lys Glu Gly Gly 
    50                  55                  60                  


Phe Glu Val Asn Gly Asn Phe Ile Lys Val Ser Ala Glu Arg Asp Pro 
65                  70                  75                  80  


Glu Asn Ile Asp Trp Ala Thr Asp Gly Val Glu Ile Val Leu Glu Ala 
                85                  90                  95      


Thr Gly Phe Phe Ala Lys Lys Glu Ala Ala Glu Lys His Leu His Thr 
            100                 105                 110         


Asn Gly Ala Lys Lys Val Val Ile Thr Ala Pro Gly Gly Asn Asp Val 
        115                 120                 125             


Lys Thr Val Val Phe Asn Thr Asn His Asp Ile Leu Asp Gly Thr Glu 
    130                 135                 140                 


Thr Val Ile Ser Gly Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro Met 
145                 150                 155                 160 


Ala Lys Ala Leu His Asp Ala Phe Gly Ile Gln Lys Gly Leu Met Thr 
                165                 170                 175     


Thr Ile His Ala Tyr Thr Gly Asp Gln Met Ile Leu Asp Gly Pro His 
            180                 185                 190         


Arg Gly Gly Asp Leu Arg Arg Ala Arg Ala Gly Ala Ala Asn Ile Val 
        195                 200                 205             


Pro Asn Ser Thr Gly Ala Ala Lys Ala Ile Gly Leu Val Ile Pro Glu 
    210                 215                 220                 


Leu Asn Gly Lys Leu Asp Gly Ala Ala Gln Arg Val Pro Val Pro Thr 
225                 230                 235                 240 


Gly Ser Val Thr Glu Leu Val Val Thr Leu Asp Lys Asn Val Ser Val 
                245                 250                 255     


Asp Glu Ile Asn Ala Ala Met Lys Ala Ala Ser Asn Asp Ser Phe Gly 
            260                 265                 270         


Tyr Thr Glu Asp Pro Ile Val Ser Ser Asp Ile Val Gly Val Ser Tyr 
        275                 280                 285             


Gly Ser Leu Phe Asp Ala Thr Gln Thr Lys Val Met Glu Val Asp Gly 
    290                 295                 300                 


Ser Gln Leu Val Lys Val Val Ser Trp Tyr Asp Asn Glu Met Ser Tyr 
305                 310                 315                 320 


Thr Ala Gln Leu Val Arg Thr Leu Glu Tyr Phe Ala Lys Ile Ala Lys 
                325                 330                 335     


<210>  21
<211>  335
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  21

Met Val Val Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Ala Phe Arg Arg Ile Gln Asn Val Glu Gly Val Glu Val Thr Arg Ile 
            20                  25                  30          


Asn Asp Leu Thr Asp Pro Val Met Leu Ala His Leu Leu Lys Tyr Asp 
        35                  40                  45              


Thr Thr Gln Gly Arg Phe Asp Gly Thr Val Glu Val Lys Glu Gly Gly 
    50                  55                  60                  


Phe Glu Val Asn Gly Lys Phe Ile Lys Val Ser Ala Glu Arg Asp Pro 
65                  70                  75                  80  


Glu Gln Ile Asp Trp Ala Thr Asp Gly Val Glu Ile Val Leu Glu Ala 
                85                  90                  95      


Thr Gly Phe Phe Ala Lys Lys Glu Ala Ala Glu Lys His Leu Lys Gly 
            100                 105                 110         


Gly Ala Lys Lys Val Val Ile Thr Ala Pro Gly Gly Asn Asp Val Lys 
        115                 120                 125             


Thr Val Val Phe Asn Thr Asn His Asp Val Leu Asp Gly Thr Glu Thr 
    130                 135                 140                 


Val Ile Ser Gly Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro Met Ala 
145                 150                 155                 160 


Lys Ala Leu Gln Asp Asn Phe Gly Val Val Glu Gly Leu Met Thr Thr 
                165                 170                 175     


Ile His Ala Tyr Thr Gly Asp Gln Met Ile Leu Asp Gly Pro His Arg 
            180                 185                 190         


Gly Gly Asp Leu Arg Arg Ala Arg Ala Gly Ala Ala Asn Ile Val Pro 
        195                 200                 205             


Asn Ser Thr Gly Ala Ala Lys Ala Ile Gly Leu Val Ile Pro Glu Leu 
    210                 215                 220                 


Asn Gly Lys Leu Asp Gly Ser Ala Gln Arg Val Pro Thr Pro Thr Gly 
225                 230                 235                 240 


Ser Val Thr Glu Leu Val Ala Val Leu Glu Lys Asn Val Thr Val Asp 
                245                 250                 255     


Glu Val Asn Ala Ala Met Lys Ala Ala Ser Asn Glu Ser Tyr Gly Tyr 
            260                 265                 270         


Thr Glu Asp Pro Ile Val Ser Ser Asp Ile Val Gly Leu Ser Tyr Gly 
        275                 280                 285             


Ser Leu Phe Asp Ala Thr Gln Thr Lys Val Leu Asp Val Asp Gly Lys 
    290                 295                 300                 


Gln Leu Val Lys Val Val Ser Trp Tyr Asp Asn Glu Met Ser Tyr Thr 
305                 310                 315                 320 


Ala Gln Leu Val Arg Thr Leu Glu Tyr Phe Ala Lys Ile Ala Lys 
                325                 330                 335 


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aminocidos 1-12 de la GAPDH de L. monocytogenes

<400>  22

Met Thr Val Lys Val Gly Ile Asn Gly Phe Gly Arg 
1               5                   10          


