                         SEQUENCE LISTING

<110>  Stichting Wageningen Research
 
<120>  POLYNUCLEOTIDE CONSTRUCTS AND METHODS OF GENE EDITING USING CPF1

<130>  P256595GB

<160>  157   

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM and recognition sequence

<400>  1
tttatgtccc ctgttgac                                                     18


<210>  2
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM and recognition sequence

<400>  2
tttaggatgc cactaaaa                                                     18


<210>  3
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM and recognition sequence

<400>  3
tttagatcga atcttcta                                                     18


<210>  4
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PAM and recognition sequence

<400>  4
tttgtgctaa cgctgatg                                                     18


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpf1 target site

<300>
<301>  Kim, D et al.
<302>  Genome-wide analysis reveals specificities of Cpf1 endonucleases 
       in human cells
<303>  Nature biotechnology
<304>  34
<305>  8
<306>  863-868
<307>  2016-08   

<400>  5
tttatgtccc ctgttgac                                                     18


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpt1 target site

<300>
<301>  Kleinstiver, B. P. et al
<302>  Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human 
       cells
<303>  Nature biotechnology
<304>  34
<305>  8
<306>  869-874
<307>  2016-08   

<400>  6
tttaggatgc cactaaaa                                                     18


<210>  7
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpt1 target site

<300>
<301>  Kleinstiver, B. P. et al
<302>  Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human 
       cells
<303>  Nature biotechnology
<304>  34
<305>  8
<306>  869-874
<307>  2016-08   

<400>  7
tttagatcga atcttcta                                                     18


<210>  8
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left1 target site

<400>  8
tttatgtccc ctgttgacca tt                                                22


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right1 target site

<400>  9
tttatgtccc ctgttgacgc tt                                                22


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left2 target site

<400>  10
tttaggatgc cactaaaaca tt                                                22


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right2 target site

<400>  11
tttaggatgc cactaaaagc tt                                                22


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Left3 target site

<400>  12
tttagatcga atcttctaca tt                                                22


<210>  13
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Right3 target site

<400>  13
tttagatcga atcttctagc tt                                                22


<210>  14
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expected CN excision

<400>  14
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  15
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Expected Positive Control

<400>  15
cattacaatt acatttacaa ttatcgatac aatggggtca tccaagaatg ttatcaagga       60


<210>  16
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-18bp-1 Forward

<400>  16
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  17
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-18bp-1 Reverse

<400>  17
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  18
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-18bp-2 Forward

<400>  18
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  19
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-18bp-2 Reverse

<400>  19
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  20
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-21bp-1 Forward

<400>  20
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  21
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-21bp-1 Reverse

<400>  21
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  22
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-21bp-2 Forward

<400>  22
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  23
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-21bp-2 Reverse

<400>  23
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  24
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-23bp-1 Forward

<400>  24
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  25
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-23bp-1 Reverse

<400>  25
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  26
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exCN1-23bp-2 Forward

<400>  26
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  27
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Location of repair

<400>  27
gatacgatac                                                              10


<210>  28
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN1-23bp-2 reverse

<400>  28
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  29
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-18bp-1 forward

<400>  29
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  30
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-18bp-1 reverse

<400>  30
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  31
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-18bp-2 forward

<400>  31
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  32
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-18bp-2 reverse

<400>  32
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  33
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-21bp-1 forward

<400>  33
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  34
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-21bp-1 reverse

<400>  34
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  35
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-21bp-2 forward

<400>  35
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  36
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-21bp-2 reverse

<400>  36
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  37
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-23bp-1 forward

<400>  37
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  38
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-23bp-1 reverse

<400>  38
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  39
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-23bp-2 forward

<400>  39
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  40
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN2-23bp-2 reverse

<400>  40
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  41
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-18bp-1 forward

<400>  41
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  42
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-18bp-1 reverse

<400>  42
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  43
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-18bp-2 forward

<400>  43
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  44
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-18bp-2 reverse

<400>  44
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  45
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-21bp-1 forward

<400>  45
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  46
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-21bp-1 reverse

<400>  46
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  47
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-21bp-2 forward

<400>  47
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  48
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-21bp-2 reverse

<400>  48
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  49
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-23bp-1 forward

<400>  49
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  50
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-23bp-1 reverse

<400>  50
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  51
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-23bp-2 forward

<400>  51
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  52
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. exCN3-23bp-2 reverse

<400>  52
cattacaatt acatttacaa ttatcgatac gatacgatac aatggggtca tccaagaatg       60

ttatcaagga                                                              70


<210>  53
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Table 2. Positive Cont-1 forward

<400>  53
cattacaatt acatttacaa ttatcgatac aatggggtca tccaagaatg ttatcaagga       60


<210>  54
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. Positive Cont-1 reverse

<400>  54
cattacaatt acatttacaa ttatcgatac aatggggtca tccaagaatg ttatcaagga       60


<210>  55
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. Positive Cont-2 forward

<400>  55
cattacaatt acatttacaa ttatcgatac aatggggtca tccaagaatg ttatcaagga       60


<210>  56
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 2. Positive Cont-2 reverse

<400>  56
cattacaatt acatttacaa ttatcgatac aatggggtca tccaagaatg ttatcaagga       60


<210>  57
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. Expected CN excision

<400>  57
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  58
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. Expected positive control

<400>  58
cattacaatt acatttacaa ttatcgatac aatgaatatt tgtactaata agtcgtcgtc       60


<210>  59
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Table3. exCN1-18bp-1 forward

<400>  59
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  60
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-18bp-1 reverse

<400>  60
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  61
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-18bp-2 forward

<400>  61
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  62
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-18bp-2 reverse

<400>  62
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  63
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-21bp-1 forward

<400>  63
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  64
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-21bp-1 reverse

<400>  64
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  65
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-21bp-2 forward

<400>  65
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  66
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-21bp-2 reverse

<400>  66
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  67
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-23bp-1  forward

<400>  67
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  68
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-23bp-1 reverse

<400>  68
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  69
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-23bp-2 forward

<400>  69
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  70
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN1-23bp-2 reverse

<400>  70
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  71
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-18bp-1forward

<400>  71
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  72
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-18bp-1 reverse

<400>  72
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  73
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-18bp-2 forward

<400>  73
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  74
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-18bp-2 reverse

<400>  74
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  75
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-21bp-1 forward

<400>  75
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  76
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-21bp-1 reverse

<400>  76
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  77
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-21bp-2 forward

<400>  77
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  78
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-21bp-2 reverse

<400>  78
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  79
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL001forward primer

<400>  79
tttgaagaca aaatggctaa taacgcttta ca                                     32


<210>  80
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-23bp-1 reverse

<400>  80
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  81
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-23bp-2 forward

<400>  81
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  82
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN2-23bp-2 reverse

<400>  82
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  83
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-18bp-1 forward

<400>  83
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  84
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-18bp-1 reverse

<400>  84
cattataatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  85
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-18bp-2 forward

<400>  85
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  86
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-18bp-2 reverse

<400>  86
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  87
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-21bp-1 forward

<400>  87
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  88
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-21bp-1 reverse

<400>  88
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  89
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-21bp-2 forward

<400>  89
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  90
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-21bp-2 reverse

<400>  90
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  91
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-23bp-1 forward

<400>  91
cattacaatt acatttacaa ttatcgatac aatgaatatt tgtactaata agtcgtcgtc       60


<210>  92
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-23bp-1 reverse

<400>  92
cattacaatt acatttacaa ttttcgatac aatgaatatt tgtactaata agtcgtcgtc       60


<210>  93
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-23bp-2 forward

<400>  93
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  94
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. exCN3-23bp-2 reverse

<400>  94
cattacaatt acatttacaa ttatcgatac gatacgatac aatgaatatt tgtactaata       60

agtcgtcgtc                                                              70


<210>  95
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. Positive Cont-1 forward

<400>  95
cattacaatt acatttacaa ttatcgatac aatgaatatt tgtactaata agtcgtcgtc       60


<210>  96
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. Positive control reverse

<400>  96
cattacaatt acatttacaa ttatcgatac aatgaatatt tgtactaata agtcgtcgtc       60


<210>  97
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. Positive Cont-2 forward

<400>  97
cattacaatt acatttacaa ttatcgatac aatgaatatt tgtactaata agtcgtcgtc       60


<210>  98
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Table 3. Positive Cont-2 reverse

<400>  98
cattacaatt acatttacaa ttatcgatac aatgaatatt tgtactaata agtcgtcgtc       60


<210>  99
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL002 reverse primer

<400>  99
tttgaagaca aaagctcaac gtttgtaatc gatgg                                  35


<210>  100
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL003 forward primer

<400>  100
tttgaagaca aatccgctgg gaacggcgaa tatgc                                  35


<210>  101
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL004 reverse primer

<400>  101
tttgaagaca aggataccac ggatcgaaca catca                                  35


<210>  102
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL005 forward primer

<400>  102
tttgaagaca aaatggctaa taacgcttta caaacaatta ttaacg                      46


<210>  103
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL006 reverse primer

<400>  103
tttgaagaca atccacgttt gtaatcgatg gct                                    33


<210>  104
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL007 forward primer

<400>  104
tttgaagaca atggatctga acaagatgga ttgcacgcag                             40


<210>  105
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL008 reverse primer

<400>  105
tttgaagaca aaagctcaga agaactcgtc aagaaggc                               38


<210>  106
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL009 forrward primer

<400>  106
tttgaagaca aaatgaatat ttgtactaat aagtcgtc                               38


<210>  107
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL010 reverse primer

<400>  107
tttgaagaca aaagctcaac tgagaagtgg cattt                                  35


<210>  108
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL011 forward primer

<400>  108
tttgaagaca aaatggtcaa caggggacat aaaggaggaa ttccaatccc aca              53


<210>  109
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL012 reverse primer

<400>  109
tttgaagaca aaagcgtcaa caggggacat aaaagcgtcg atctagtaac ata              53


<210>  110
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL013 forward primer

<400>  110
tttgaagaca aaatgtttta gtggcatcct aaaggaggaa ttccaatccc aca              53


<210>  111
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL014 reverse primer

<400>  111
tttgaagaca aaagctttta gtggcatcct aaaagcgtcg atctagtaac ata              53


<210>  112
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL015 forward primer

<400>  112
tttgaagaca aaatgtagaa gattcgatct aaaggaggaa ttccaatccc aca              53


<210>  113
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL016 reverse primer

<400>  113
tttgaagaca aaagctagaa gattcgatct aaaagcgtcg atctagtaac ata              53


<210>  114
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL017 forward primer

<400>  114
tttgaagaca agcttgatac gatacgatac aatggggtca tccaagaatg ttatca           56


<210>  115
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL018 forward primer

<400>  115
tttgaagaca agcttgatac gatacgatac aatgaatatt tgtactaata agtcgtc          57


<210>  116
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCL019 reverse primer

<400>  116
tttgaagaca aagcggataa tttatttgaa aattcat                                37


<210>  117
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS001 primer

<400>  117
agcgagtcag tgagcgag                                                     18


<210>  118
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS002 primer

<400>  118
aataggcgta tcacgaggc                                                    19


<210>  119
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS003 primer

<400>  119
gctttgtcga aaccgttgct                                                   20


<210>  120
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS004 primer

<400>  120
gtgtgctggc aatcaccttg                                                   20


<210>  121
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS005 primer

<400>  121
tgggcacaac agacaatcgg ctgc                                              24


<210>  122
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS006 primer

<400>  122
tgcgaatcgg gagcggcgat accg                                              24


<210>  123
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS007 primer

<400>  123
aaagtcccac atcgatcagg t                                                 21


<210>  124
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS008 primer

<400>  124
ggtggcagga tatattgtgg                                                   20


<210>  125
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS009 primer

<400>  125
tgcacataca aatggacgaa c                                                 21


<210>  126
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS010 primer

<400>  126
ccactatcct tcgcaagacc                                                   20


<210>  127
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS011 primer

<400>  127
gtgctccacc atgttgacga                                                   20


<210>  128
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS012

<400>  128
tggagaggac acgctcgagt                                                   20


<210>  129
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS013 primer

<400>  129
gcaactgtgc tgttaagctc                                                   20


<210>  130
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS014 primer

<400>  130
gctggcacat acaaatggac                                                   20


<210>  131
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS015 primer

<400>  131
gcgcgcggtg tcatctatgt                                                   20


<210>  132
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS016 primer

<400>  132
aatgtgcgtg gctttatctg tc                                                22


<210>  133
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS017 primer

<400>  133
gagcgccaca ataacaaaca                                                   20


<210>  134
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS018 primer

<400>  134
acgaggagca tcgtggaaaa a                                                 21


<210>  135
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS019 primer

<400>  135
ctggtatgtc ggcagggtg                                                    19


<210>  136
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRSS020 primer

<400>  136
agcttatgaa gcctcaaaat gaga                                              24


<210>  137
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR01 primer

<400>  137
tgtggtctca ggagtgatca aaagtcccac atc                                    33


<210>  138
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR02 primer

<400>  138
tgtggtctca attgaatttc tactaagtgt agatggaact gaaagtcaag atggtcattt       60

ttcgcttgag accaca                                                       76


<210>  139
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR03 primer

<400>  139
tgtggtctca agcgaaaaat gaccatcttg actttcagtt ccatctacac ttagtagaaa       60

ttcaattgag accaca                                                       76


<210>  140
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR04 primer

<400>  140
tgtggtctca agcgaaaaaa atggtcaaca ggggacaatc tacacttagt agaaattcaa       60

tcgctatgt                                                               69


<210>  141
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR05 primer

<400>  141
tgtggtctca agcgaaaaaa atgttttagt ggcatccatc tacacttagt agaaattcaa       60

tcgctatgt                                                               69


<210>  142
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR06 primer

<400>  142
tgtggtctca agcgaaaaaa atgtagaaga ttcgatcatc tacacttagt agaaattcaa       60

tcgctatgt                                                               69


<210>  143
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR07 primer

<400>  143
tgtggtctca agcgaaaaaa agcgtcaaca ggggacaatc tacacttagt agaaattcaa       60

tcgctatgt                                                               69


<210>  144
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR08 primer

<400>  144
tgtggtctca agcgaaaaaa agcttttagt ggcatccatc tacacttagt agaaattcaa       60

tcgctatgt                                                               69


<210>  145
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR09 primer

<400>  145
tgtggtctca agcgaaaaaa agctagaaga ttcgatcatc tacacttagt agaaattcaa       60

tcgctatgt                                                               69


<210>  146
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR10 primer

<400>  146
tgtggtctca agcgaaaaat acaatggtca acaggggaca atctacactt agtagaaatt       60

caatcgctat gt                                                           72


<210>  147
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR11 primer

<400>  147
tgtggtctca agcgaaaaat acaatgtttt agtggcatcc atctacactt agtagaaatt       60

caatcgctat gt                                                           72


<210>  148
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR12 primer

<400>  148
tgtggtctca agcgaaaaat acaatgtaga agattcgatc atctacactt agtagaaatt       60

caatcgctat gt                                                           72


<210>  149
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR13 primer

<400>  149
tgtggtctca agcgaaaaaa tcaagcgtca acaggggaca atctacactt agtagaaatt       60

caatcgctat gt                                                           72


<210>  150
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR14 primer

<400>  150
tgtggtctca agcgaaaaaa tcaagctttt agtggcatcc atctacactt agtagaaatt       60

caatcgctat gt                                                           72


<210>  151
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR15 primer

<400>  151
tgtggtctca agcgaaaaaa tcaagctaga agattcgatc atctacactt agtagaaatt       60

caatcgctat gt                                                           72


<210>  152
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR16 primer

<400>  152
tgtggtctca agcgaaaaag atacaatggt caacagggga caatctacac ttagtagaaa       60

ttcaatcgct atgt                                                         74


<210>  153
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR17 primer

<400>  153
tgtggtctca agcgaaaaag atacaatgtt ttagtggcat ccatctacac ttagtagaaa       60

ttcaatcgct atgt                                                         74


<210>  154
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR18 primer

<400>  154
tgtggtctca agcgaaaaag atacaatgta gaagattcga tcatctacac ttagtagaaa       60

ttcaatcgct atgt                                                         74


<210>  155
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR19 primer

<400>  155
tgtggtctca agcgaaaaag tatcaagcgt caacagggga caatctacac ttagtagaaa       60

ttcaatcgct atgt                                                         74


<210>  156
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR20 primer

<400>  156
tgtggtctca agcgaaaaag tatcaagctt ttagtggcat ccatctacac ttagtagaaa       60

ttcaatcgct atgt                                                         74


<210>  157
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PRCR21 primer

<400>  157
tgtggtctca agcgaaaaag tatcaagcta gaagattcga tcatctacac ttagtagaaa       60

ttcaatcgct atgt                                                         74


