                         SEQUENCE LISTING

<110>  acib GmbH
 
<120>  Methionine analogue synthesis

<130>  ACI15995PCT

<150>  LU 100833
<151>  2018-06-13

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  434
<212>  PRT
<213>  Geobacillus stearothermophilus

<400>  1

Met Ser Asn Glu Gln Thr Phe Arg Pro Glu Thr Leu Ala Ile His Ala 
1               5                   10                  15      


Gly Gln Lys Pro Asp Ala Glu Thr Gly Ala Arg Ala Val Pro Ile Tyr 
            20                  25                  30          


Gln Thr Ser Ser Tyr Val Phe Arg Asp Ser Glu His Ala Ala Asn Leu 
        35                  40                  45              


Phe Gly Leu Lys Glu Glu Gly Phe Ile Tyr Thr Arg Ile Met Asn Pro 
    50                  55                  60                  


Thr Asn Asp Val Leu Glu Lys Arg Ile Ala Ala Leu Glu Gly Gly Ile 
65                  70                  75                  80  


Gly Ala Leu Ala Leu Ser Ser Gly Gln Ala Ala Val Phe Tyr Ser Ile 
                85                  90                  95      


Ile Asn Ile Ala Ser Ala Gly Asp Glu Ile Val Ser Ser Ser Ser Ile 
            100                 105                 110         


Tyr Gly Gly Thr Tyr Asn Leu Phe Ala His Thr Leu Arg Lys Phe Gly 
        115                 120                 125             


Ile Thr Val Lys Phe Val Asp Pro Ser Asp Pro Glu Asn Phe Glu Arg 
    130                 135                 140                 


Ala Ile Thr Asp Lys Thr Lys Ala Leu Phe Ala Glu Thr Ile Gly Asn 
145                 150                 155                 160 


Pro Lys Asn Asp Val Leu Asp Ile Glu Ala Val Ala Asp Ile Ala His 
                165                 170                 175     


Arg His Ala Ile Pro Leu Ile Val Asp Asn Thr Val Ala Ser Pro Tyr 
            180                 185                 190         


Leu Leu Arg Pro Ile Glu Phe Gly Ala Asp Ile Val Val His Ser Ala 
        195                 200                 205             


Thr Lys Phe Ile Gly Gly His Gly Asn Ser Ile Gly Gly Val Ile Val 
    210                 215                 220                 


Asp Ser Gly Lys Phe Asp Trp Lys Gly Ser Gly Lys Phe Pro Glu Phe 
225                 230                 235                 240 


Thr Glu Pro Asp Pro Ser Tyr His Gly Leu Val Tyr Val Asp Ala Val 
                245                 250                 255     


Gly Glu Ala Ala Tyr Ile Thr Lys Ala Arg Ile Gln Leu Leu Arg Asp 
            260                 265                 270         


Leu Gly Ala Ala Leu Ser Pro Phe Asn Ala Phe Leu Leu Leu Gln Gly 
        275                 280                 285             


Leu Glu Thr Leu His Leu Arg Met Gln Arg His Ser Glu Asn Ala Leu 
    290                 295                 300                 


Ala Val Ala Lys Phe Leu Glu Glu Glu Glu Ala Val Glu Ser Val Asn 
305                 310                 315                 320 


Tyr Pro Gly Leu Pro Ser His Pro Ser His Glu Leu Ala Lys Lys Tyr 
                325                 330                 335     


Leu Pro Asn Gly Gln Gly Ala Ile Val Thr Phe Glu Ile Lys Gly Gly 
            340                 345                 350         


Val Glu Ala Gly Lys Lys Leu Ile Asp Ser Val Lys Leu Phe Ser His 
        355                 360                 365             


Leu Ala Asn Ile Gly Asp Ser Lys Ser Leu Ile Ile His Pro Ala Ser 
    370                 375                 380                 


Thr Thr His Glu Gln Leu Thr Pro Glu Glu Gln Leu Ser Ala Gly Val 
385                 390                 395                 400 


Thr Pro Gly Leu Val Arg Leu Ser Val Gly Thr Glu Ala Ile Asp Asp 
                405                 410                 415     


Ile Leu Asp Asp Leu Arg Gln Ala Ile Arg Gln Ser Gln Thr Val Gly 
            420                 425                 430         


Val Lys 
        


<210>  2
<211>  334
<212>  PRT
<213>  Deinococcus radiodurans

<400>  2

Met Thr Ala Val Leu Ala Gly His Ala Ser Ala Leu Leu Leu Thr Glu 
1               5                   10                  15      


Glu Pro Asp Cys Ser Gly Pro Gln Thr Val Val Leu Phe Arg Arg Glu 
            20                  25                  30          


Pro Leu Leu Leu Asp Cys Gly Arg Ala Leu Ser Asp Val Arg Val Ala 
        35                  40                  45              


Phe His Thr Tyr Gly Thr Pro Arg Ala Asp Ala Thr Leu Val Leu His 
    50                  55                  60                  


Ala Leu Thr Gly Asp Ser Ala Val His Glu Trp Trp Pro Asp Phe Leu 
65                  70                  75                  80  


Gly Ala Gly Arg Pro Leu Asp Pro Ala Asp Asp Tyr Val Val Cys Ala 
                85                  90                  95      


Asn Val Leu Gly Gly Cys Ala Gly Thr Thr Ser Ala Ala Glu Leu Ala 
            100                 105                 110         


Ala Thr Cys Ser Gly Pro Val Pro Leu Ser Leu Arg Asp Met Ala Arg 
        115                 120                 125             


Val Gly Arg Ala Leu Leu Asp Ser Leu Gly Val Arg Arg Val Arg Val 
    130                 135                 140                 


Ile Gly Ala Ser Met Gly Gly Met Leu Ala Tyr Ala Trp Leu Leu Glu 
145                 150                 155                 160 


Cys Pro Asp Leu Val Glu Lys Ala Val Ile Ile Gly Ala Pro Ala Arg 
                165                 170                 175     


His Ser Pro Trp Ala Ile Gly Leu Asn Thr Ala Ala Arg Ser Ala Ile 
            180                 185                 190         


Ala Leu Ala Pro Gly Gly Glu Gly Leu Lys Val Ala Arg Gln Ile Ala 
        195                 200                 205             


Met Leu Ser Tyr Arg Ser Pro Glu Ser Leu Ser Arg Thr Gln Ala Gly 
    210                 215                 220                 


Gln Arg Val Pro Gly Val Pro Ala Val Thr Ser Tyr Leu His Tyr Gln 
225                 230                 235                 240 


Gly Glu Lys Leu Ala Ala Arg Phe Asp Glu Gln Thr Tyr Cys Ala Leu 
                245                 250                 255     


Thr Trp Ala Met Asp Ala Phe Gln Pro Ser Ser Ala Asp Leu Lys Ala 
            260                 265                 270         


Val Arg Ala Pro Val Leu Val Val Gly Ile Ser Ser Asp Leu Leu Tyr 
        275                 280                 285             


Pro Ala Ala Glu Val Arg Ala Cys Ala Ala Glu Leu Pro His Ala Asp 
    290                 295                 300                 


Tyr Trp Glu Leu Gly Ser Ile His Gly His Asp Ala Phe Leu Met Asp 
305                 310                 315                 320 


Pro Gln Asp Leu Pro Glu Arg Val Gly Ala Phe Leu Arg Ser 
                325                 330                 


<210>  3
<211>  380
<212>  PRT
<213>  Mycobacterium smegmatis

<400>  3

Met Thr Ile Ile Glu Glu Arg Ala Thr Asp Thr Gly Met Ala Thr Val 
1               5                   10                  15      


Pro Leu Pro Ala Glu Gly Glu Ile Gly Leu Val His Ile Gly Ala Leu 
            20                  25                  30          


Thr Leu Glu Asn Gly Thr Val Leu Pro Asp Val Thr Ile Ala Val Gln 
        35                  40                  45              


Arg Trp Gly Glu Leu Ala Pro Asp Arg Gly Asn Val Val Met Val Leu 
    50                  55                  60                  


His Ala Leu Thr Gly Asp Ser His Val Thr Gly Pro Ala Gly Asp Gly 
65                  70                  75                  80  


His Pro Thr Ala Gly Trp Trp Asp Gly Val Ala Gly Pro Gly Ala Pro 
                85                  90                  95      


Ile Asp Thr Asp His Trp Cys Ala Ile Ala Thr Asn Val Leu Gly Gly 
            100                 105                 110         


Cys Arg Gly Ser Thr Gly Pro Gly Ser Leu Ala Pro Asp Gly Lys Pro 
        115                 120                 125             


Trp Gly Ser Arg Phe Pro Gln Ile Thr Ile Arg Asp Gln Val Ala Ala 
    130                 135                 140                 


Asp Arg Ala Ala Leu Ala Ala Leu Gly Ile Thr Glu Val Ala Ala Val 
145                 150                 155                 160 


Val Gly Gly Ser Met Gly Gly Ala Arg Ala Leu Glu Trp Leu Val Thr 
                165                 170                 175     


His Pro Asp Asp Val Arg Ala Gly Leu Val Leu Ala Val Gly Ala Arg 
            180                 185                 190         


Ala Thr Ala Asp Gln Ile Gly Thr Gln Ser Thr Gln Val Ala Ala Ile 
        195                 200                 205             


Lys Ala Asp Pro Asp Trp Gln Gly Gly Asp Tyr His Gly Thr Gly Arg 
    210                 215                 220                 


Ala Pro Thr Glu Gly Met Glu Ile Ala Arg Arg Phe Ala His Leu Thr 
225                 230                 235                 240 


Tyr Arg Gly Glu Glu Glu Leu Asp Asp Arg Phe Ala Asn Thr Pro Gln 
                245                 250                 255     


Asp Asp Glu Asp Pro Leu Thr Gly Gly Arg Tyr Ala Val Gln Ser Tyr 
            260                 265                 270         


Leu Glu Tyr Gln Gly Gly Lys Leu Ala Arg Arg Phe Asp Pro Gly Thr 
        275                 280                 285             


Tyr Val Val Leu Ser Asp Ala Leu Ser Ser His Asp Val Gly Arg Gly 
    290                 295                 300                 


Arg Gly Gly Val Glu Ala Ala Leu Arg Ser Cys Pro Val Pro Val Val 
305                 310                 315                 320 


Val Gly Gly Ile Thr Ser Asp Arg Leu Tyr Pro Ile Arg Leu Gln Gln 
                325                 330                 335     


Glu Leu Ala Glu Leu Leu Pro Gly Cys Gln Gly Leu Asp Val Val Asp 
            340                 345                 350         


Ser Ile Tyr Gly His Asp Gly Phe Leu Val Glu Thr Glu Leu Val Gly 
        355                 360                 365             


Lys Leu Ile Arg Arg Thr Leu Glu Leu Ala Gln Arg 
    370                 375                 380 


<210>  4
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide primer pBP1471

<400>  4
ctgagtagga caaatccgcc gtcgacaaat cataaaaaat ttatttgctt tgtgagcgg        59


<210>  5
<211>  59
<212>  DNA
<213>  artificial

<220>
<223>  oligonucleotide primer pBP1472

<400>  5
agaggcccca aggggttatg ctagcattta aatctatcag tgatggtgat ggtgatggg        59


<210>  6
<211>  477
<212>  DNA
<213>  Clostridium beijerinckii

<400>  6
atgtggagcc acccgcagtt cgaaaaagga tccatgaaaa tcgtatattg gtctggtacc       60

ggcaacactg agaaaatggc agagctcatc gctaaaggta tcatcgaatc tggtaaagac      120

gtcaacacca tcaacgtgtc tgacgttaac atcgatgaac tgctgaacga agatatcctg      180

atcctgggtt gctctgccat gggcgatgaa gttctcgagg aaagcgaatt tgaaccgttc      240

atcgaagaga tctctaccaa aatctctggt aagaaggttg cgctgttcgg ttcttatggt      300

tggggcgacg gtaagtggat gcgtgacttc gaagaacgta tgaacggcta tggttgcgtt      360

gttgttgaga ccccgctgat cgttcagaac gagccggacg aagctgagca ggactgcatc      420

gaatttggta agaagatcgc gaacatcgga tcccatcacc atcaccatca ctgatag         477


