                               SEQUENCE LISTING

<110> IGNITE BIOSCIENCES, INC.
 
<120> METHODS AND APPLICATIONS OF PROTEIN IDENTIFICATION

<130> 51612-702.601

<140> PCT/US2019/035654
<141> 2019-06-05

<150> 62/681,644
<151> 2018-06-06

<160> 16    

<170> PatentIn version 3.5

<210> 1
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 1
Met Lys Leu Thr Leu Lys Asn Leu Ser Met Ala Ile Met Met Ser Ile 
1               5                   10                  15      


Val Met Gly Ser Ser Ala Met Ala Ala Asp Ser Asn Glu Lys 
            20                  25                  30  


<210> 2
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 2
Gly Tyr Leu Pro Glu His Thr Leu 
1               5               


<210> 3
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 3
Ala Asp Tyr Leu Glu Gln Asp 
1               5           


<210> 4
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 4
Thr Tyr Pro Gly Arg 
1               5   


<210> 5
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 5
Leu His Asp His Tyr Leu Asp 
1               5           


<210> 6
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 6
Asp Arg Ala Arg Lys Asp Gly 
1               5           


<210> 7
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 7
Thr Phe Glu Glu Glu 
1               5   


<210> 8
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 8
Asp Glu Ile Lys Ser Leu Lys Phe 
1               5               


<210> 9
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Gln Thr Tyr Pro Gly Arg Phe Pro Met Gly 
1               5                   10  


<210> 10
<211> 18
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
His Thr Phe Glu Glu Glu Ile Glu Phe Val Gln Gly Leu Asn His Ser 
1               5                   10                  15      


Thr Gly 
        


<210> 11
<211> 40
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 11
Asn Ile Gly Ile Tyr Pro Glu Ile Lys Ala Pro Trp Phe His Gln Glu 
1               5                   10                  15      


Gly Lys Asp Ile Ala Ala Lys Thr Leu Glu Val Leu Lys Lys Tyr Gly 
            20                  25                  30          


Tyr Thr Gly Lys Asp Asp Lys Val 
        35                  40  


<210> 12
<211> 244
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 
    50                  55                  60                  


Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys His His 
225                 230                 235                 240 


His His His His 
                


<210> 13
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 13
His His His His His His 
1               5       


<210> 14
<211> 150
<212> PRT
<213> Bos taurus

<400> 14
Met Ala Leu Lys Ser Leu Val Leu Leu Ser Leu Leu Val Leu Val Leu 
1               5                   10                  15      


Leu Leu Val Arg Val Gln Pro Ser Leu Gly Lys Glu Thr Ala Ala Ala 
            20                  25                  30          


Lys Phe Glu Arg Gln His Met Asp Ser Ser Thr Ser Ala Ala Ser Ser 
        35                  40                  45              


Ser Asn Tyr Cys Asn Gln Met Met Lys Ser Arg Asn Leu Thr Lys Asp 
    50                  55                  60                  


Arg Cys Lys Pro Val Asn Thr Phe Val His Glu Ser Leu Ala Asp Val 
65                  70                  75                  80  


Gln Ala Val Cys Ser Gln Lys Asn Val Ala Cys Lys Asn Gly Gln Thr 
                85                  90                  95      


Asn Cys Tyr Gln Ser Tyr Ser Thr Met Ser Ile Thr Asp Cys Arg Glu 
            100                 105                 110         


Thr Gly Ser Ser Lys Tyr Pro Asn Cys Ala Tyr Lys Thr Thr Gln Ala 
        115                 120                 125             


Asn Lys His Ile Ile Val Ala Cys Glu Gly Asn Pro Tyr Val Pro Val 
    130                 135                 140                 


His Phe Asp Ala Ser Val 
145                 150 


<210> 15
<211> 710
<212> PRT
<213> Homo sapiens

<400> 15
Met Lys Leu Val Phe Leu Val Leu Leu Phe Leu Gly Ala Leu Gly Leu 
1               5                   10                  15      


Cys Leu Ala Gly Arg Arg Arg Ser Val Gln Trp Cys Ala Val Ser Gln 
            20                  25                  30          


Pro Glu Ala Thr Lys Cys Phe Gln Trp Gln Arg Asn Met Arg Lys Val 
        35                  40                  45              


Arg Gly Pro Pro Val Ser Cys Ile Lys Arg Asp Ser Pro Ile Gln Cys 
    50                  55                  60                  


Ile Gln Ala Ile Ala Glu Asn Arg Ala Asp Ala Val Thr Leu Asp Gly 
65                  70                  75                  80  


Gly Phe Ile Tyr Glu Ala Gly Leu Ala Pro Tyr Lys Leu Arg Pro Val 
                85                  90                  95      


Ala Ala Glu Val Tyr Gly Thr Glu Arg Gln Pro Arg Thr His Tyr Tyr 
            100                 105                 110         


Ala Val Ala Val Val Lys Lys Gly Gly Ser Phe Gln Leu Asn Glu Leu 
        115                 120                 125             


Gln Gly Leu Lys Ser Cys His Thr Gly Leu Arg Arg Thr Ala Gly Trp 
    130                 135                 140                 


Asn Val Pro Ile Gly Thr Leu Arg Pro Phe Leu Asn Trp Thr Gly Pro 
145                 150                 155                 160 


Pro Glu Pro Ile Glu Ala Ala Val Ala Arg Phe Phe Ser Ala Ser Cys 
                165                 170                 175     


Val Pro Gly Ala Asp Lys Gly Gln Phe Pro Asn Leu Cys Arg Leu Cys 
            180                 185                 190         


Ala Gly Thr Gly Glu Asn Lys Cys Ala Phe Ser Ser Gln Glu Pro Tyr 
        195                 200                 205             


Phe Ser Tyr Ser Gly Ala Phe Lys Cys Leu Arg Asp Gly Ala Gly Asp 
    210                 215                 220                 


Val Ala Phe Ile Arg Glu Ser Thr Val Phe Glu Asp Leu Ser Asp Glu 
225                 230                 235                 240 


Ala Glu Arg Asp Glu Tyr Glu Leu Leu Cys Pro Asp Asn Thr Arg Lys 
                245                 250                 255     


Pro Val Asp Lys Phe Lys Asp Cys His Leu Ala Arg Val Pro Ser His 
            260                 265                 270         


Ala Val Val Ala Arg Ser Val Asn Gly Lys Glu Asp Ala Ile Trp Asn 
        275                 280                 285             


Leu Leu Arg Gln Ala Gln Glu Lys Phe Gly Lys Asp Lys Ser Pro Lys 
    290                 295                 300                 


Phe Gln Leu Phe Gly Ser Pro Ser Gly Gln Lys Asp Leu Leu Phe Lys 
305                 310                 315                 320 


Asp Ser Ala Ile Gly Phe Ser Arg Val Pro Pro Arg Ile Asp Ser Gly 
                325                 330                 335     


Leu Tyr Leu Gly Ser Gly Tyr Phe Thr Ala Ile Gln Asn Leu Arg Lys 
            340                 345                 350         


Ser Glu Glu Glu Val Ala Ala Arg Arg Ala Arg Val Val Trp Cys Ala 
        355                 360                 365             


Val Gly Glu Gln Glu Leu Arg Lys Cys Asn Gln Trp Ser Gly Leu Ser 
    370                 375                 380                 


Glu Gly Ser Val Thr Cys Ser Ser Ala Ser Thr Thr Glu Asp Cys Ile 
385                 390                 395                 400 


Ala Leu Val Leu Lys Gly Glu Ala Asp Ala Met Ser Leu Asp Gly Gly 
                405                 410                 415     


Tyr Val Tyr Thr Ala Gly Lys Cys Gly Leu Val Pro Val Leu Ala Glu 
            420                 425                 430         


Asn Tyr Lys Ser Gln Gln Ser Ser Asp Pro Asp Pro Asn Cys Val Asp 
        435                 440                 445             


Arg Pro Val Glu Gly Tyr Leu Ala Val Ala Val Val Arg Arg Ser Asp 
    450                 455                 460                 


Thr Ser Leu Thr Trp Asn Ser Val Lys Gly Lys Lys Ser Cys His Thr 
465                 470                 475                 480 


Ala Val Asp Arg Thr Ala Gly Trp Asn Ile Pro Met Gly Leu Leu Phe 
                485                 490                 495     


Asn Gln Thr Gly Ser Cys Lys Phe Asp Glu Tyr Phe Ser Gln Ser Cys 
            500                 505                 510         


Ala Pro Gly Ser Asp Pro Arg Ser Asn Leu Cys Ala Leu Cys Ile Gly 
        515                 520                 525             


Asp Glu Gln Gly Glu Asn Lys Cys Val Pro Asn Ser Asn Glu Arg Tyr 
    530                 535                 540                 


Tyr Gly Tyr Thr Gly Ala Phe Arg Cys Leu Ala Glu Asn Ala Gly Asp 
545                 550                 555                 560 


Val Ala Phe Val Lys Asp Val Thr Val Leu Gln Asn Thr Asp Gly Asn 
                565                 570                 575     


Asn Asn Glu Ala Trp Ala Lys Asp Leu Lys Leu Ala Asp Phe Ala Leu 
            580                 585                 590         


Leu Cys Leu Asp Gly Lys Arg Lys Pro Val Thr Glu Ala Arg Ser Cys 
        595                 600                 605             


His Leu Ala Met Ala Pro Asn His Ala Val Val Ser Arg Met Asp Lys 
    610                 615                 620                 


Val Glu Arg Leu Lys Gln Val Leu Leu His Gln Gln Ala Lys Phe Gly 
625                 630                 635                 640 


Arg Asn Gly Ser Asp Cys Pro Asp Lys Phe Cys Leu Phe Gln Ser Glu 
                645                 650                 655     


Thr Lys Asn Leu Leu Phe Asn Asp Asn Thr Glu Cys Leu Ala Arg Leu 
            660                 665                 670         


His Gly Lys Thr Thr Tyr Glu Lys Tyr Leu Gly Pro Gln Tyr Val Ala 
        675                 680                 685             


Gly Ile Thr Asn Leu Lys Lys Cys Ser Thr Ser Pro Leu Leu Glu Ala 
    690                 695                 700                 


Cys Glu Phe Leu Arg Lys 
705                 710 


<210> 16
<211> 243
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 16
Ser Thr Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val 
1               5                   10                  15      


Gln Pro Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu 
            20                  25                  30          


His Leu Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe 
        35                  40                  45              


Glu Leu Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp 
    50                  55                  60                  


Val Lys Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys 
65                  70                  75                  80  


His Asn Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met 
                85                  90                  95      


Leu Glu Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala 
            100                 105                 110         


Tyr Ser Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu 
        115                 120                 125             


Pro Glu Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr 
    130                 135                 140                 


Leu Asn Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala 
145                 150                 155                 160 


Leu Asp Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro 
                165                 170                 175     


Lys Leu Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp 
            180                 185                 190         


Lys Tyr Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp 
        195                 200                 205             


Gln Ala Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Leu Val 
    210                 215                 220                 


Pro Arg Gly Ser Pro Leu Glu Val Leu Phe Gln Gly Pro Cys Gly Ser 
225                 230                 235                 240 


Tyr Gly Ser 
            


