﻿                         序列表

<110>  中国农业科学院饲料研究所

<120>  葡萄糖氧化酶GOD突变体及其基因和应用

<160>  6

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  581
<212>  PRT
<213>  黑曲霉(Aspergillus niger)

<400>  1
Gly Ile Glu Ala Ser Leu Leu Thr Asp Pro Lys Glu Val Ala Gly Arg 
1               5                   10                  15      
Thr Val Asp Tyr Ile Ile Ala Gly Gly Gly Leu Thr Gly Leu Thr Thr 
            20                  25                  30          
Ala Ala Arg Leu Thr Glu Asn Pro Asp Ile Thr Val Leu Val Ile Glu 
        35                  40                  45              
Ser Gly Ser Tyr Glu Ser Asp Arg Gly Pro Ile Ile Glu Asp Leu Asn 
    50                  55                  60                  
Ala Tyr Gly Asp Ile Phe Gly Ser Ser Val Asp His Ala Tyr Glu Thr 
65                  70                  75                  80  
Val Glu Leu Ala Thr Asn Asn Gln Thr Ala Leu Ile Arg Ser Gly Asn 
                85                  90                  95      
Gly Leu Gly Gly Ser Thr Leu Val Asn Gly Gly Thr Trp Thr Arg Pro 
            100                 105                 110         
His Lys Ala Gln Val Asp Ser Trp Glu Thr Val Phe Gly Asn Glu Gly 
        115                 120                 125             
Trp Asn Trp Asp Ser Val Ala Ala Tyr Ser Leu Gln Ala Glu Arg Ala 
    130                 135                 140                 
Arg Ala Pro Asn Ala Lys Gln Ile Ala Ala Gly His Tyr Phe Asn Ala 
145                 150                 155                 160 
Ser Cys His Gly Ile Asn Gly Thr Val His Ala Gly Pro Arg Asp Thr 
                165                 170                 175     
Gly Asp Asp Tyr Ser Pro Ile Val Lys Ala Leu Met Ser Ala Val Glu 
            180                 185                 190         
Asp Arg Gly Val Pro Thr Lys Lys Asp Leu Gly Cys Gly Asp Pro His 
        195                 200                 205             
Gly Val Ser Met Phe Pro Asn Thr Leu His Glu Asp Gln Val Arg Ser 
    210                 215                 220                 
Asp Ala Ala Arg Glu Trp Leu Leu Pro Asn Tyr Gln Arg Pro Asn Leu 
225                 230                 235                 240 
Gln Val Leu Thr Gly Gln Tyr Val Gly Lys Val Leu Leu Ser Gln Asn 
                245                 250                 255     
Ala Thr Thr Pro Arg Ala Val Gly Val Glu Phe Gly Thr His Lys Gly 
            260                 265                 270         
Asn Thr His Asn Val Tyr Ala Lys His Glu Val Leu Leu Ala Ala Gly 
        275                 280                 285             
Ser Ala Val Ser Pro Thr Ile Leu Glu Tyr Ser Gly Ile Gly Met Lys 
    290                 295                 300                 
Ser Ile Leu Glu Pro Leu Gly Ile Asp Thr Val Val Asp Leu Pro Val 
305                 310                 315                 320 
Gly Leu Asn Leu Gln Asp Gln Thr Thr Ser Thr Val Arg Ser Arg Ile 
                325                 330                 335     
Thr Ser Ala Gly Ala Gly Gln Gly Gln Ala Ala Trp Phe Ala Thr Phe 
            340                 345                 350         
Asn Glu Thr Phe Gly Asp Tyr Thr Glu Lys Ala His Glu Leu Leu Asn 
        355                 360                 365             
Thr Lys Leu Glu Gln Trp Ala Glu Glu Ala Val Ala Arg Gly Gly Phe 
    370                 375                 380                 
His Asn Thr Thr Ala Leu Leu Ile Gln Tyr Glu Asn Tyr Arg Asp Trp 
385                 390                 395                 400 
Ile Val Lys Asp Asn Val Ala Tyr Ser Glu Leu Phe Leu Asp Thr Ala 
                405                 410                 415     
Gly Val Ala Ser Phe Asp Val Trp Asp Leu Leu Pro Phe Thr Arg Gly 
            420                 425                 430         
Tyr Val His Ile Leu Asp Lys Asp Pro Tyr Leu Arg His Phe Ala Tyr 
        435                 440                 445             
Asp Pro Gln Tyr Phe Leu Asn Glu Leu Asp Leu Leu Gly Gln Ala Ala 
    450                 455                 460                 
Ala Thr Gln Leu Ala Arg Asn Ile Ser Asn Ser Gly Ala Met Gln Thr 
465                 470                 475                 480 
Tyr Phe Ala Gly Glu Thr Ile Pro Gly Asp Asn Leu Ala Tyr Asp Ala 
                485                 490                 495     
Asp Leu Arg Ala Trp Val Glu Tyr Ile Pro Tyr Asn Phe Arg Pro Asn 
            500                 505                 510         
Tyr His Gly Val Gly Thr Cys Ser Met Met Pro Lys Glu Met Gly Gly 
        515                 520                 525             
Val Val Asp Asn Ala Ala Arg Val Tyr Gly Val Gln Gly Leu Arg Val 
    530                 535                 540                 
Ile Asp Gly Ser Ile Pro Pro Thr Gln Met Ser Ser His Val Met Thr 
545                 550                 555                 560 
Val Phe Tyr Ala Met Ala Leu Lys Ile Ala Asp Ala Val Leu Ala Asp 
                565                 570                 575     
Tyr Ala Ser Met Gln 
            580     

<210>  2
<211>  581
<212>  PRT
<213>  黑曲霉(Aspergillus niger)

<400>  2
Gly Ile Glu Ala Ser Leu Leu Thr Asp Pro Lys Glu Val Ala Gly Arg 
1               5                   10                  15      
Thr Val Asp Tyr Ile Ile Ala Gly Gly Gly Leu Thr Gly Leu Thr Thr 
            20                  25                  30          
Ala Ala Arg Leu Thr Glu Asn Pro Asp Ile Thr Val Leu Val Ile Glu 
        35                  40                  45              
Ser Gly Ser Tyr Glu Ser Asp Arg Gly Pro Ile Ile Glu Asp Leu Asn 
    50                  55                  60                  
Ala Tyr Gly Asp Ile Phe Gly Ser Ser Val Asp His Ala Tyr Glu Thr 
65                  70                  75                  80  
Val Cys Leu Ala Thr Asn Asn Gln Thr Ala Leu Ile Arg Ser Gly Asn 
                85                  90                  95      
Gly Leu Gly Gly Ser Thr Leu Val Asn Gly Gly Thr Trp Thr Arg Pro 
            100                 105                 110         
His Lys Ala Gln Val Asp Ser Trp Glu Thr Val Phe Gly Asn Glu Gly 
        115                 120                 125             
Trp Asn Trp Asp Ser Val Ala Ala Tyr Ser Leu Gln Ala Glu Arg Ala 
    130                 135                 140                 
Arg Ala Pro Asn Ala Lys Gln Ile Ala Ala Gly His Tyr Phe Asn Ala 
145                 150                 155                 160 
Ser Cys His Gly Ile Asn Gly Thr Val His Ala Gly Pro Arg Asp Thr 
                165                 170                 175     
Gly Asp Asp Tyr Ser Pro Ile Val Lys Ala Leu Met Ser Ala Val Glu 
            180                 185                 190         
Asp Arg Gly Val Pro Thr Lys Lys Asp Leu Gly Cys Gly Asp Pro His 
        195                 200                 205             
Gly Val Ser Met Phe Pro Asn Thr Leu His Glu Asp Gln Val Arg Ser 
    210                 215                 220                 
Asp Ala Ala Arg Glu Trp Leu Leu Pro Asn Tyr Gln Arg Pro Asn Leu 
225                 230                 235                 240 
Gln Val Leu Thr Gly Gln Tyr Val Gly Lys Val Leu Leu Ser Gln Asn 
                245                 250                 255     
Ala Thr Thr Pro Arg Ala Val Gly Val Glu Phe Gly Thr His Lys Gly 
            260                 265                 270         
Asn Thr His Asn Val Tyr Ala Lys His Glu Val Leu Leu Ala Ala Gly 
        275                 280                 285             
Ser Ala Val Ser Pro Thr Ile Leu Glu Tyr Ser Gly Ile Gly Met Lys 
    290                 295                 300                 
Ser Ile Leu Glu Pro Leu Gly Ile Asp Thr Val Val Asp Leu Pro Val 
305                 310                 315                 320 
Gly Leu Asn Leu Gln Asp Gln Thr Thr Ser Thr Val Arg Ser Arg Ile 
                325                 330                 335     
Thr Ser Ala Gly Ala Gly Gln Gly Gln Ala Ala Trp Phe Ala Thr Phe 
            340                 345                 350         
Asn Glu Thr Phe Gly Asp Tyr Thr Glu Lys Ala His Glu Leu Leu Asn 
        355                 360                 365             
Thr Lys Leu Glu Gln Trp Ala Glu Glu Ala Val Ala Arg Gly Gly Phe 
    370                 375                 380                 
His Asn Thr Thr Ala Leu Leu Ile Gln Tyr Glu Asn Tyr Arg Asp Trp 
385                 390                 395                 400 
Ile Val Lys Asp Asn Val Ala Tyr Ser Glu Leu Phe Leu Asp Thr Ala 
                405                 410                 415     
Gly Val Ala Ser Phe Asp Val Trp Asp Leu Leu Pro Phe Thr Arg Gly 
            420                 425                 430         
Tyr Val His Ile Leu Asp Lys Asp Pro Tyr Leu Arg His Phe Ala Tyr 
        435                 440                 445             
Asp Pro Gln Tyr Phe Leu Asn Glu Leu Asp Leu Leu Gly Gln Ala Ala 
    450                 455                 460                 
Ala Thr Gln Leu Ala Arg Asn Ile Ser Asn Ser Gly Ala Met Gln Thr 
465                 470                 475                 480 
Tyr Phe Ala Gly Glu Thr Ile Pro Gly Asp Asn Leu Ala Tyr Asp Ala 
                485                 490                 495     
Asp Leu Arg Ala Trp Val Glu Tyr Ile Pro Tyr Asn Phe Arg Pro Asn 
            500                 505                 510         
Tyr His Gly Val Gly Thr Cys Ser Met Met Pro Lys Glu Met Gly Gly 
        515                 520                 525             
Val Val Asp Asn Ala Ala Arg Val Tyr Gly Val Gln Gly Leu Arg Val 
    530                 535                 540                 
Ile Asp Gly Ser Ile Pro Pro Thr Gln Met Ser Ser His Val Met Thr 
545                 550                 555                 560 
Val Phe Tyr Ala Met Ala Leu Lys Ile Ala Asp Ala Val Leu Ala Asp 
                565                 570                 575     
Tyr Ala Ser Met Gln 
            580     

<210>  3
<211>  581
<212>  PRT
<213>  黑曲霉(Aspergillus niger)

<400>  3
Gly Ile Glu Ala Ser Leu Leu Thr Asp Pro Lys Glu Val Ala Gly Arg 
1               5                   10                  15      
Thr Val Asp Tyr Ile Ile Ala Gly Gly Gly Leu Thr Gly Leu Thr Thr 
            20                  25                  30          
Ala Ala Arg Leu Thr Glu Asn Pro Asp Ile Thr Val Leu Val Ile Glu 
        35                  40                  45              
Ser Gly Ser Tyr Glu Ser Asp Arg Gly Pro Ile Ile Glu Asp Leu Asn 
    50                  55                  60                  
Ala Tyr Gly Asp Ile Phe Gly Ser Ser Val Asp His Ala Tyr Glu Thr 
65                  70                  75                  80  
Val Cys Leu Ala Thr Asn Asn Gln Thr Ala Leu Ile Arg Ser Gly Asn 
                85                  90                  95      
Gly Leu Gly Gly Ser Thr Leu Val Asn Gly Gly Thr Trp Thr Arg Pro 
            100                 105                 110         
His Lys Ala Gln Val Asp Ser Trp Glu Thr Val Phe Gly Asn Glu Gly 
        115                 120                 125             
Trp Asn Trp Asp Ser Val Ala Ala Tyr Ser Leu Gln Ala Glu Arg Ala 
    130                 135                 140                 
Arg Ala Pro Asn Ala Lys Gln Ile Ala Ala Gly His Tyr Phe Asn Ala 
145                 150                 155                 160 
Ser Cys His Gly Ile Asn Gly Thr Val His Ala Gly Pro Arg Asp Thr 
                165                 170                 175     
Gly Asp Asp Tyr Ser Pro Ile Val Lys Ala Leu Met Ser Ala Val Glu 
            180                 185                 190         
Asp Arg Gly Val Pro Thr Lys Lys Asp Leu Gly Cys Gly Asp Pro His 
        195                 200                 205             
Gly Val Ser Met Phe Pro Asn Thr Leu His Glu Asp Gln Val Arg Ser 
    210                 215                 220                 
Asp Ala Ala Arg Glu Trp Leu Leu Pro Asn Tyr Gln Arg Pro Asn Leu 
225                 230                 235                 240 
Gln Val Leu Thr Gly Gln Tyr Val Gly Lys Val Leu Leu Ser Gln Asn 
                245                 250                 255     
Ala Thr Thr Pro Arg Ala Val Gly Val Glu Phe Gly Thr His Lys Gly 
            260                 265                 270         
Asn Thr His Asn Val Tyr Ala Lys His Glu Val Leu Leu Ala Ala Gly 
        275                 280                 285             
Ser Ala Val Ser Pro Thr Ile Leu Glu Tyr Ser Gly Ile Gly Met Lys 
    290                 295                 300                 
Ser Ile Leu Glu Pro Leu Gly Ile Asp Thr Val Val Asp Leu Pro Val 
305                 310                 315                 320 
Gly Leu Asn Leu Gln Asp Gln Thr Thr Ser Thr Val Arg Ser Arg Ile 
                325                 330                 335     
Thr Ser Ala Gly Ala Gly Gln Gly Gln Ala Ala Trp Phe Ala Thr Phe 
            340                 345                 350         
Asn Glu Thr Phe Gly Asp Tyr Thr Glu Lys Ala His Glu Leu Leu Asn 
        355                 360                 365             
Thr Lys Leu Glu Gln Trp Ala Glu Glu Ala Val Ala Arg Gly Gly Phe 
    370                 375                 380                 
His Asn Thr Thr Ala Leu Leu Ile Gln Tyr Glu Asn Tyr Arg Asp Trp 
385                 390                 395                 400 
Ile Val Lys Asp Asn Val Ala Tyr Ser Glu Leu Phe Leu Asp Thr Ala 
                405                 410                 415     
Gly Glu Ala Ser Phe Asp Val Trp Asp Leu Leu Pro Phe Thr Arg Gly 
            420                 425                 430         
Tyr Val His Ile Leu Asp Lys Asp Pro Tyr Leu Arg His Phe Ala Tyr 
        435                 440                 445             
Asp Pro Gln Tyr Phe Leu Asn Glu Leu Asp Leu Leu Gly Gln Ala Ala 
    450                 455                 460                 
Ala Thr Gln Leu Ala Arg Asn Ile Ser Asn Ser Gly Ala Met Gln Thr 
465                 470                 475                 480 
Tyr Phe Ala Gly Glu Thr Ile Pro Gly Asp Asn Leu Ala Tyr Asp Ala 
                485                 490                 495     
Asp Leu Arg Ala Trp Val Glu Tyr Ile Pro Tyr Asn Phe Arg Pro Asn 
            500                 505                 510         
Tyr His Gly Val Gly Thr Cys Ser Met Met Pro Lys Glu Met Gly Gly 
        515                 520                 525             
Val Val Asp Asn Ala Ala Arg Val Tyr Gly Val Gln Gly Leu Arg Val 
    530                 535                 540                 
Ile Asp Gly Ser Ile Pro Pro Thr Gln Met Ser Ser His Val Met Thr 
545                 550                 555                 560 
Val Phe Tyr Ala Met Ala Leu Lys Ile Ala Asp Ala Val Leu Ala Asp 
                565                 570                 575     
Tyr Ala Ser Met Gln 
            580     

<210>  4
<211>  581
<212>  PRT
<213>  黑曲霉(Aspergillus niger)

<400>  4
Gly Ile Glu Ala Ser Leu Leu Thr Asp Pro Lys Glu Val Ala Gly Arg 
1               5                   10                  15      
Thr Val Asp Tyr Ile Ile Ala Gly Gly Gly Leu Thr Gly Leu Thr Thr 
            20                  25                  30          
Ala Ala Arg Leu Thr Glu Asn Pro Asp Ile Thr Val Leu Val Ile Glu 
        35                  40                  45              
Ser Gly Ser Tyr Glu Ser Asp Arg Gly Pro Ile Ile Glu Asp Leu Asn 
    50                  55                  60                  
Ala Tyr Gly Asp Ile Phe Gly Ser Ser Val Asp His Ala Tyr Glu Thr 
65                  70                  75                  80  
Val Cys Leu Ala Thr Asn Asn Gln Thr Ala Leu Ile Arg Ser Gly Asn 
                85                  90                  95      
Gly Leu Gly Gly Ser Thr Leu Val Asn Gly Gly Thr Trp Thr Arg Pro 
            100                 105                 110         
His Lys Ala Gln Val Asp Ser Trp Glu Thr Val Phe Gly Asn Glu Gly 
        115                 120                 125             
Trp Asn Trp Asp Ser Val Ala Ala Tyr Ser Leu Gln Ala Glu Arg Ala 
    130                 135                 140                 
Arg Ala Pro Asn Ala Lys Gln Ile Ala Ala Gly His Tyr Phe Asn Ala 
145                 150                 155                 160 
Ser Cys His Gly Ile Asn Gly Thr Val His Ala Gly Pro Arg Asp Thr 
                165                 170                 175     
Gly Asp Asp Tyr Ser Pro Ile Val Lys Ala Leu Met Ser Ala Val Glu 
            180                 185                 190         
Asp Arg Gly Val Pro Thr Lys Lys Asp Leu Gly Cys Gly Asp Pro His 
        195                 200                 205             
Gly Val Ser Met Phe Pro Asn Thr Leu His Glu Asp Gln Val Arg Ser 
    210                 215                 220                 
Asp Ala Ala Arg Glu Trp Leu Leu Pro Asn Tyr Gln Arg Pro Asn Leu 
225                 230                 235                 240 
Gln Val Leu Thr Gly Gln Tyr Val Gly Lys Val Leu Leu Ser Gln Asn 
                245                 250                 255     
Ala Thr Thr Pro Arg Ala Val Gly Val Glu Phe Gly Thr His Lys Gly 
            260                 265                 270         
Asn Thr His Asn Val Tyr Ala Lys His Glu Val Leu Leu Ala Ala Gly 
        275                 280                 285             
Ser Ala Val Ser Pro Thr Ile Leu Glu Tyr Ser Gly Ile Gly Met Lys 
    290                 295                 300                 
Ser Ile Leu Glu Pro Leu Gly Ile Asp Thr Val Val Asp Leu Pro Val 
305                 310                 315                 320 
Gly Leu Asn Leu Gln Asp Gln Thr Thr Ser Thr Val Arg Ser Arg Ile 
                325                 330                 335     
Thr Ser Ala Gly Ala Gly Gln Gly Gln Ala Ala Trp Phe Ala Thr Phe 
            340                 345                 350         
Asn Glu Thr Phe Gly Asp Tyr Thr Glu Lys Ala His Glu Leu Leu Asn 
        355                 360                 365             
Thr Lys Leu Glu Gln Trp Ala Glu Glu Ala Val Ala Arg Gly Gly Phe 
    370                 375                 380                 
His Asn Thr Thr Ala Leu Leu Ile Gln Tyr Glu Asn Tyr Arg Asp Trp 
385                 390                 395                 400 
Ile Val Lys Asp Asn Val Ala Tyr Ser Glu Leu Phe Leu Asp Thr Ala 
                405                 410                 415     
Gly Glu Ala Ser Phe Asp Val Trp Asp Leu Leu Pro Phe Thr Arg Gly 
            420                 425                 430         
Tyr Val His Ile Leu Asp Lys Asp Pro Tyr Leu Arg His Phe Ala Tyr 
        435                 440                 445             
Asp Pro Gln Tyr Phe Leu Asn Glu Leu Asp Leu Leu Gly Gln Ala Ala 
    450                 455                 460                 
Ala Thr Gln Leu Ala Arg Asn Ile Ser Asn Ser Gly Ala Met Gln Thr 
465                 470                 475                 480 
Tyr Phe Ala Gly Glu Thr Ile Pro Gly Asp Asn Leu Ala Tyr Asp Ala 
                485                 490                 495     
Asp Leu Arg Ala Trp Val Glu Tyr Ile Pro Tyr His Phe Arg Pro Asn 
            500                 505                 510         
Tyr His Gly Val Gly Thr Cys Ser Met Met Pro Lys Glu Met Gly Gly 
        515                 520                 525             
Val Val Asp Asn Ala Ala Arg Val Tyr Gly Val Gln Gly Leu Arg Val 
    530                 535                 540                 
Ile Asp Gly Ser Ile Pro Pro Thr Gln Met Ser Ser His Val Met Thr 
545                 550                 555                 560 
Val Phe Tyr Ala Met Ala Leu Lys Ile Ala Asp Ala Val Leu Ala Asp 
                565                 570                 575     
Tyr Ala Ser Met Gln 
            580     

<210>  5
<211>  581
<212>  PRT
<213>  黑曲霉(Aspergillus niger)

<400>  5
Gly Ile Glu Ala Ser Leu Leu Thr Asp Pro Lys Glu Val Ala Gly Arg 
1               5                   10                  15      
Thr Val Asp Tyr Ile Ile Ala Gly Gly Gly Leu Thr Gly Leu Thr Val 
            20                  25                  30          
Ala Ala Arg Leu Thr Glu Asn Pro Asp Ile Thr Val Leu Val Ile Glu 
        35                  40                  45              
Ser Gly Ser Tyr Glu Ser Asp Arg Gly Pro Ile Ile Glu Asp Leu Asn 
    50                  55                  60                  
Ala Tyr Gly Asp Ile Phe Gly Ser Ser Val Asp His Ala Tyr Glu Thr 
65                  70                  75                  80  
Val Cys Leu Ala Thr Asn Asn Gln Thr Ala Leu Ile Arg Ser Gly Asn 
                85                  90                  95      
Gly Leu Gly Gly Ser Thr Leu Val Asn Gly Gly Thr Trp Thr Arg Pro 
            100                 105                 110         
His Lys Ala Gln Val Asp Ser Trp Glu Thr Val Phe Gly Asn Glu Gly 
        115                 120                 125             
Trp Asn Trp Asp Ser Val Ala Ala Tyr Ser Leu Gln Ala Glu Arg Ala 
    130                 135                 140                 
Arg Ala Pro Asn Ala Lys Gln Ile Ala Ala Gly His Tyr Phe Asn Ala 
145                 150                 155                 160 
Ser Cys His Gly Ile Asn Gly Thr Val His Ala Gly Pro Arg Asp Thr 
                165                 170                 175     
Gly Asp Asp Tyr Ser Pro Ile Val Lys Ala Leu Met Ser Ala Val Glu 
            180                 185                 190         
Asp Arg Gly Val Pro Thr Lys Lys Asp Leu Gly Cys Gly Asp Pro His 
        195                 200                 205             
Gly Val Ser Met Phe Pro Asn Thr Leu His Glu Asp Gln Val Arg Ser 
    210                 215                 220                 
Asp Ala Ala Arg Glu Trp Leu Leu Pro Asn Tyr Gln Arg Pro Asn Leu 
225                 230                 235                 240 
Gln Val Leu Thr Gly Gln Tyr Val Gly Lys Val Leu Leu Ser Gln Asn 
                245                 250                 255     
Ala Thr Thr Pro Arg Ala Val Gly Val Glu Phe Gly Thr His Lys Gly 
            260                 265                 270         
Asn Thr His Asn Val Tyr Ala Lys His Glu Val Leu Leu Ala Ala Gly 
        275                 280                 285             
Ser Ala Val Ser Pro Thr Ile Leu Glu Tyr Ser Gly Ile Gly Met Lys 
    290                 295                 300                 
Ser Ile Leu Glu Pro Leu Gly Ile Asp Thr Val Val Asp Leu Pro Val 
305                 310                 315                 320 
Gly Leu Asn Leu Gln Asp Gln Thr Thr Ser Thr Val Arg Ser Arg Ile 
                325                 330                 335     
Thr Ser Ala Gly Ala Gly Gln Gly Gln Ala Ala Trp Phe Ala Thr Phe 
            340                 345                 350         
Asn Glu Thr Phe Gly Asp Tyr Thr Glu Lys Ala His Glu Leu Leu Asn 
        355                 360                 365             
Thr Lys Leu Glu Gln Trp Ala Glu Glu Ala Val Ala Arg Gly Gly Phe 
    370                 375                 380                 
His Asn Thr Thr Ala Leu Leu Ile Gln Tyr Glu Asn Tyr Arg Asp Trp 
385                 390                 395                 400 
Ile Val Lys Asp Asn Val Ala Tyr Ser Glu Leu Phe Leu Asp Thr Ala 
                405                 410                 415     
Gly Glu Ala Ser Phe Asp Val Trp Asp Leu Leu Pro Phe Thr Arg Gly 
            420                 425                 430         
Tyr Val His Ile Leu Asp Lys Asp Pro Tyr Leu Arg His Phe Ala Tyr 
        435                 440                 445             
Asp Pro Gln Tyr Phe Leu Asn Glu Leu Asp Leu Leu Gly Gln Ala Ala 
    450                 455                 460                 
Ala Thr Gln Leu Ala Arg Asn Ile Ser Asn Ser Gly Ala Met Gln Thr 
465                 470                 475                 480 
Tyr Phe Ala Gly Glu Thr Ile Pro Gly Asp Asn Leu Ala Tyr Asp Ala 
                485                 490                 495     
Asp Leu Arg Ala Trp Val Glu Tyr Ile Pro Tyr His Phe Arg Pro Asn 
            500                 505                 510         
Tyr His Gly Val Gly Thr Cys Ser Met Met Pro Lys Glu Met Gly Gly 
        515                 520                 525             
Val Val Asp Asn Ala Ala Arg Val Tyr Gly Val Gln Gly Leu Arg Val 
    530                 535                 540                 
Ile Asp Gly Ser Ile Pro Pro Thr Gln Met Ser Ser His Val Met Thr 
545                 550                 555                 560 
Val Phe Tyr Ala Met Ala Leu Lys Ile Ala Asp Ala Val Leu Ala Asp 
                565                 570                 575     
Tyr Ala Ser Met Gln 
            580     

<210>  6
<211>  581
<212>  PRT
<213>  黑曲霉(Aspergillus niger)

<400>  6
Gly Ile Glu Ala Ser Leu Leu Thr Asp Pro Lys Glu Val Ala Gly Arg 
1               5                   10                  15      
Thr Val Asp Tyr Ile Ile Ala Gly Gly Gly Leu Thr Gly Leu Thr Val 
            20                  25                  30          
Ala Ala Arg Leu Thr Glu Asn Pro Asp Ile Thr Val Leu Val Ile Glu 
        35                  40                  45              
Ser Gly Ser Tyr Glu Ser Asp Arg Gly Pro Ile Ile Glu Asp Leu Asn 
    50                  55                  60                  
Ala Tyr Gly Asp Ile Phe Gly Ser Ser Val Asp His Ala Tyr Glu Thr 
65                  70                  75                  80  
Val Cys Leu Ala Thr Asn Asn Gln Thr Ala Leu Ile Arg Ser Gly Asn 
                85                  90                  95      
Gly Leu Gly Gly Ser Thr Leu Val Asn Gly Gly Thr Trp Thr Arg Pro 
            100                 105                 110         
His Lys Ala Gln Val Asp Ser Trp Glu Thr Val Phe Gly Asn Glu Gly 
        115                 120                 125             
Trp Asn Trp Asp Ser Val Ala Ala Tyr Ser Leu Gln Ala Glu Arg Ala 
    130                 135                 140                 
Arg Ala Pro Asn Ala Lys Gln Ile Ala Ala Gly His Tyr Phe Asn Ala 
145                 150                 155                 160 
Ser Cys His Gly Ile Asn Gly Thr Val His Ala Gly Pro Arg Asp Thr 
                165                 170                 175     
Gly Asp Asp Tyr Ser Pro Ile Val Lys Ala Leu Met Ser Ala Val Glu 
            180                 185                 190         
Asp Arg Gly Val Pro Thr Lys Lys Asp Leu Gly Cys Gly Asp Pro His 
        195                 200                 205             
Gly Val Ser Met Phe Pro Asn Thr Leu His Glu Asp Gln Val Arg Ser 
    210                 215                 220                 
Asp Ala Ala Arg Glu Trp Leu Leu Pro Asn Tyr Gln Arg Pro Asn Leu 
225                 230                 235                 240 
Gln Val Leu Thr Gly Gln Tyr Val Gly Lys Val Leu Leu Ser Gln Asn 
                245                 250                 255     
Ala Thr Thr Pro Arg Ala Val Gly Val Glu Phe Gly Thr His Lys Gly 
            260                 265                 270         
Asn Thr His Asn Val Tyr Ala Lys His Glu Val Leu Leu Ala Ala Gly 
        275                 280                 285             
Ser Ala Val Ser Pro Thr Ile Leu Glu Tyr Ser Gly Ile Gly Met Lys 
    290                 295                 300                 
Ser Ile Leu Glu Pro Leu Gly Ile Lys Thr Val Val Asp Leu Pro Val 
305                 310                 315                 320 
Gly Leu Asn Leu Gln Asp Gln Thr Thr Ser Thr Val Arg Ser Arg Ile 
                325                 330                 335     
Thr Ser Ala Gly Ala Gly Gln Gly Gln Ala Ala Trp Phe Ala Thr Phe 
            340                 345                 350         
Asn Glu Thr Phe Gly Asp Tyr Thr Glu Lys Ala His Glu Leu Leu Asn 
        355                 360                 365             
Thr Lys Leu Glu Gln Trp Ala Glu Glu Ala Val Ala Arg Gly Gly Phe 
    370                 375                 380                 
His Asn Thr Thr Ala Leu Leu Ile Gln Tyr Glu Asn Tyr Arg Asp Trp 
385                 390                 395                 400 
Ile Val Lys Asp Asn Val Ala Tyr Ser Glu Leu Phe Leu Asp Thr Ala 
                405                 410                 415     
Gly Glu Ala Ser Phe Asp Val Trp Asp Leu Leu Pro Phe Thr Arg Gly 
            420                 425                 430         
Tyr Val His Ile Leu Asp Lys Asp Pro Tyr Leu Arg His Phe Ala Tyr 
        435                 440                 445             
Asp Pro Gln Tyr Phe Leu Asn Glu Leu Asp Leu Leu Gly Gln Ala Ala 
    450                 455                 460                 
Ala Thr Gln Leu Ala Arg Asn Ile Ser Asn Ser Gly Ala Met Gln Thr 
465                 470                 475                 480 
Tyr Phe Ala Gly Glu Thr Ile Pro Gly Asp Asn Leu Ala Tyr Asp Ala 
                485                 490                 495     
Asp Leu Arg Ala Trp Val Glu Tyr Ile Pro Tyr His Phe Arg Pro Asn 
            500                 505                 510         
Tyr His Gly Val Gly Thr Cys Ser Met Met Pro Lys Glu Met Gly Gly 
        515                 520                 525             
Val Val Asp Asn Ala Ala Arg Val Tyr Gly Val Gln Gly Leu Arg Val 
    530                 535                 540                 
Ile Asp Gly Ser Ile Pro Pro Thr Gln Met Ser Ser His Val Met Thr 
545                 550                 555                 560 
Val Phe Tyr Ala Met Ala Leu Lys Ile Ala Asp Ala Val Leu Ala Asp 
                565                 570                 575     
Tyr Ala Ser Met Gln 
            580     

