                               SEQUENCE LISTING

<110> ROCHE SEQUENCING SOLUTIONS, INC.
      ROCHE DIAGNOSTICS GMBH
      F. HOFFMANN-LA ROCHE AG

<120> ENZYMATIC ENRICHMENT OF DNA-PORE-POLYMERASE COMPLEXES

<130> P34805-WO

<140>
<141>

<150> 62/677,142
<151> 2018-05-28

<160> 12    

<170> PatentIn version 3.5

<210> 1
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"


<220>
<221> SITE
<222> (1)..(15) 
<223> /note="This sequence may encompass 1-3 'Gly Gly Gly Gly Ser'
      repeating units"

<400> 1
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210> 2
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 2
Arg Ser Lys Leu Gly 
1               5   


<210> 3
<211> 727
<212> PRT
<213> Clostridium phage phiCPV4

<400> 3
Met Asp Lys His Thr Gln Tyr Val Lys Glu His Ser Phe Asn Tyr Asp 
1               5                   10                  15      


Glu Tyr Lys Lys Ala Asn Phe Asp Lys Ile Glu Cys Leu Ile Phe Asp 
            20                  25                  30          


Thr Glu Ser Cys Thr Asn Tyr Glu Asn Asp Asn Thr Gly Ala Arg Val 
        35                  40                  45              


Tyr Gly Trp Gly Leu Gly Val Thr Arg Asn His Asn Met Ile Tyr Gly 
    50                  55                  60                  


Gln Asn Leu Asn Gln Phe Trp Glu Val Cys Gln Asn Ile Phe Asn Asp 
65                  70                  75                  80  


Trp Tyr His Asp Asn Lys His Thr Ile Lys Ile Thr Lys Thr Lys Lys 
                85                  90                  95      


Gly Phe Pro Lys Arg Lys Tyr Ile Lys Phe Pro Ile Ala Val His Asn 
            100                 105                 110         


Leu Gly Trp Asp Val Glu Phe Leu Lys Tyr Ser Leu Val Glu Asn Gly 
        115                 120                 125             


Phe Asn Tyr Asp Lys Gly Leu Leu Lys Thr Val Phe Ser Lys Gly Ala 
    130                 135                 140                 


Pro Tyr Gln Thr Val Thr Asp Val Glu Glu Pro Lys Thr Phe His Ile 
145                 150                 155                 160 


Val Gln Asn Asn Asn Ile Val Tyr Gly Cys Asn Val Tyr Met Asp Lys 
                165                 170                 175     


Phe Phe Glu Val Glu Asn Lys Asp Gly Ser Thr Thr Glu Ile Gly Leu 
            180                 185                 190         


Cys Leu Asp Phe Phe Asp Ser Tyr Lys Ile Ile Thr Cys Ala Glu Ser 
        195                 200                 205             


Gln Phe His Asn Tyr Val His Asp Val Asp Pro Met Phe Tyr Lys Met 
    210                 215                 220                 


Gly Glu Glu Tyr Asp Tyr Asp Thr Trp Arg Ser Pro Thr His Lys Gln 
225                 230                 235                 240 


Thr Thr Leu Glu Leu Arg Tyr Gln Tyr Asn Asp Ile Tyr Met Leu Arg 
                245                 250                 255     


Glu Val Ile Glu Gln Phe Tyr Ile Asp Gly Leu Cys Gly Gly Glu Leu 
            260                 265                 270         


Pro Leu Thr Gly Met Arg Thr Ala Ser Ser Ile Ala Phe Asn Val Leu 
        275                 280                 285             


Lys Lys Met Thr Phe Gly Glu Glu Lys Thr Glu Glu Gly Tyr Ile Asn 
    290                 295                 300                 


Tyr Phe Glu Leu Asp Lys Lys Thr Lys Phe Glu Phe Leu Arg Lys Arg 
305                 310                 315                 320 


Ile Glu Met Glu Ser Tyr Thr Gly Gly Tyr Thr His Ala Asn His Lys 
                325                 330                 335     


Ala Val Gly Lys Thr Ile Asn Lys Ile Gly Cys Ser Leu Asp Ile Asn 
            340                 345                 350         


Ser Ser Tyr Pro Ser Gln Met Ala Tyr Lys Val Phe Pro Tyr Gly Lys 
        355                 360                 365             


Pro Val Arg Lys Thr Trp Gly Arg Lys Pro Lys Thr Glu Lys Asn Glu 
    370                 375                 380                 


Val Tyr Leu Ile Glu Val Gly Phe Asp Phe Val Glu Pro Lys His Glu 
385                 390                 395                 400 


Glu Tyr Ala Leu Asp Ile Phe Lys Ile Gly Ala Val Asn Ser Lys Ala 
                405                 410                 415     


Leu Ser Pro Ile Thr Gly Ala Val Ser Gly Gln Glu Tyr Phe Cys Thr 
            420                 425                 430         


Asn Ile Lys Asp Gly Lys Ala Ile Pro Val Tyr Lys Glu Leu Lys Asp 
        435                 440                 445             


Thr Lys Leu Thr Thr Asn Tyr Asn Val Val Leu Thr Ser Val Glu Tyr 
    450                 455                 460                 


Glu Phe Trp Ile Lys His Phe Asn Phe Gly Val Phe Lys Lys Asp Glu 
465                 470                 475                 480 


Tyr Asp Cys Phe Glu Val Asp Asn Leu Glu Phe Thr Gly Leu Lys Ile 
                485                 490                 495     


Gly Ser Ile Leu Tyr Tyr Lys Ala Glu Lys Gly Lys Phe Lys Pro Tyr 
            500                 505                 510         


Val Asp His Phe Thr Lys Met Lys Val Glu Asn Lys Lys Leu Gly Asn 
        515                 520                 525             


Lys Pro Leu Thr Asn Gln Ala Lys Leu Ile Leu Asn Gly Ala Tyr Gly 
    530                 535                 540                 


Lys Phe Gly Thr Lys Gln Asn Lys Glu Glu Lys Asp Leu Ile Met Asp 
545                 550                 555                 560 


Lys Asn Gly Leu Leu Thr Phe Thr Gly Ser Val Thr Glu Tyr Glu Gly 
                565                 570                 575     


Lys Glu Phe Tyr Arg Pro Tyr Ala Ser Phe Val Thr Ala Tyr Gly Arg 
            580                 585                 590         


Leu Gln Leu Trp Asn Ala Ile Ile Tyr Ala Val Gly Val Glu Asn Phe 
        595                 600                 605             


Leu Tyr Cys Asp Thr Asp Ser Ile Tyr Cys Asn Arg Glu Val Asn Ser 
    610                 615                 620                 


Leu Ile Glu Asp Met Asn Ala Ile Gly Glu Thr Ile Asp Lys Thr Ile 
625                 630                 635                 640 


Leu Gly Lys Trp Asp Val Glu His Val Phe Asp Lys Phe Lys Val Leu 
                645                 650                 655     


Gly Gln Lys Lys Tyr Met Tyr His Asp Cys Lys Glu Asp Lys Thr Asp 
            660                 665                 670         


Leu Lys Cys Cys Gly Leu Pro Ser Asp Ala Arg Lys Ile Ile Ile Gly 
        675                 680                 685             


Gln Gly Phe Asp Glu Phe Tyr Leu Gly Lys Asn Val Glu Gly Lys Lys 
    690                 695                 700                 


Gln Arg Lys Lys Val Ile Gly Gly Cys Leu Leu Leu Asp Thr Leu Phe 
705                 710                 715                 720 


Thr Ile Lys Lys Ile Met Phe 
                725         


<210> 4
<211> 305
<212> PRT
<213> Staphylococcus aureus

<400> 4
Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Thr Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn Gly Leu Ser Ala Trp Ser His Pro Gln Phe Glu 
    290                 295                 300                 


Lys 
305 


<210> 5
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 5
Ala His Ile Val Met Val Asp Ala Tyr Lys 
1               5                   10  


<210> 6
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 6
Gly Ser Ser Gly Gly Ser Ser Gly Gly 
1               5                   


<210> 7
<211> 13
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 7
Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210> 8
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 8
Lys Gly His His His His His His 
1               5               


<210> 9
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 9
Gly Ser Ser Gly Gly Ser Ser Gly Gly Ala His Ile Val Met Val Asp 
1               5                   10                  15      


Ala Tyr Lys Pro Thr Lys Lys Gly His His His His His His 
            20                  25                  30  


<210> 10
<211> 5466
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polynucleotide"

<400> 10
cagtcagtag agagagagat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat       60

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac      120

gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa      180

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat      240

gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc      300

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg      360

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag      420

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc      480

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag      540

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga      600

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc      660

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg      720

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt      780

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca      840

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca      900

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag      960

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     1020

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     1080

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     1140

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     1200

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     1260

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     1320

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     1380

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     1440

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     1500

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     1560

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     1620

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     1680

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     1740

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     1800

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     1860

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     1920

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     1980

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     2040

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     2100

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     2160

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg     2220

tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct     2280

ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga     2340

cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag     2400

cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga     2460

gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca     2520

ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt     2580

cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc     2640

cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc     2700

ggggatctct ctctcaaaaa cggaggagga ggacagtcag tagagagaga gatccccggg     2760

taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg     2820

ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag     2880

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga     2940

tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca     3000

gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg     3060

acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct     3120

ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg     3180

gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt     3240

caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac     3300

attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa     3360

aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat     3420

tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc     3480

agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga     3540

gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg     3600

cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc     3660

agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag     3720

taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc     3780

tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg     3840

taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg     3900

acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac     3960

ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac     4020

cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg     4080

agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg     4140

tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg     4200

agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac     4260

tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg     4320

ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg     4380

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc     4440

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc     4500

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt     4560

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc     4620

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact     4680

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac     4740

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag     4800

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg     4860

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg     4920

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga     4980

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt     5040

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct     5100

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg     5160

aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt     5220

aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta     5280

atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta     5340

tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt     5400

acgccaagct tgcatgcctg caggtcgact ctagaggatc tctctctcaa aaacggagga     5460

ggagga                                                                5466


<210> 11
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 11
atctctctct actgactgtc ctcctcctcc gttttt                                 36


<210> 12
<211> 10
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 12
gagagagatt                                                              10


