                         SEQUENCE LISTING

<110>  Henkel AG & CO. KGaA
 
<120>  Verbesserte Waschleistung durch eine neue alpha-Amylase aus 
       Fomitopsis pinicola (Fpi)

<130>  2018P35104WO

<150>  DE102018208445.9
<151>  2018-05-29

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  576
<212>  PRT
<213>  Fomitopsis pinicola

<400>  1

Ala Thr Thr Ala Glu Trp Gln Gln Arg Ser Ile Tyr Gln Leu Val Thr 
1               5                   10                  15      


Asp Arg Phe Ala Thr Ser Asp Gly Ser Ser Pro Ala Cys Asp Thr Gly 
            20                  25                  30          


Asp Arg Val Tyr Cys Gly Gly Ser Trp Gln Gly Val Ile Asn Lys Leu 
        35                  40                  45              


Asp Tyr Ile Gln Tyr Met Gly Phe Asp Ala Ile Trp Ile Ser Pro Val 
    50                  55                  60                  


Val Lys Asn Leu Glu Gly Ser Thr Gly Asp Gly Tyr Ser Tyr His Gly 
65                  70                  75                  80  


Tyr Trp Ala Val Asp Gln Asn Ser Val Asn Glu His Phe Gly Thr Ala 
                85                  90                  95      


Asp Asp Leu Asn Ala Leu Ser Ser Ala Leu His Ala Arg Gly Met Tyr 
            100                 105                 110         


Leu Met Val Asp Val Val Val Asn His Met Ala Ala Asn Thr Leu Pro 
        115                 120                 125             


Pro Asp Tyr Ser Thr Phe Thr Pro Phe Ser Ser Glu Ser Asp Phe His 
    130                 135                 140                 


Thr Phe Cys Trp Ile Thr Asp Tyr Asp Asn Gln Thr Asn Val Glu Gln 
145                 150                 155                 160 


Cys Trp Leu Gly Asp Ser Ser Val Pro Leu Ala Asp Cys Asp Thr Glu 
                165                 170                 175     


Ala Asp Asn Val Ile Asp Phe Phe Tyr Asn Trp Ile Gly Glu Leu Arg 
            180                 185                 190         


Ala Asn Tyr Thr Val Asp Gly Phe Arg Ile Asp Thr Leu Lys His Val 
        195                 200                 205             


Arg Gln Thr Phe Trp Pro Asp Phe Gln Thr Asn Ala Gly Val Tyr Ala 
    210                 215                 220                 


Val Gly Glu Val Phe Asp Gly Asp Val Asn Tyr Val Ser Pro Tyr Thr 
225                 230                 235                 240 


Glu Val Ile Asp Gly Val Leu Asp Tyr Pro Thr Tyr Tyr Gln Leu Thr 
                245                 250                 255     


Ser Ala Phe Glu Ser Thr Ser Gly Ser Ile Gln Asn Leu Val Asp Val 
            260                 265                 270         


Ile Gln Ser Ala Gln Ser Thr Tyr Ser Thr Met Leu Phe Gln Val Ala 
        275                 280                 285             


Thr Phe Leu Glu Asn Gln Asp Asn Pro Arg Phe Gln Ser Leu Thr Thr 
    290                 295                 300                 


Asp Gln Gly Leu Val Lys Asn Ala Met Ala Trp Pro Phe Ile Ala Asp 
305                 310                 315                 320 


Gly Ile Pro Ile Leu Tyr Tyr Gly Gln Glu Gln Gly Tyr Thr Gly Gly 
                325                 330                 335     


Asn Asp Pro Asp Asn Arg Glu Ala Leu Trp Leu Ser Gly Tyr Glu Glu 
            340                 345                 350         


Asn Lys Pro Leu Val Gln His Ala Arg Ile Leu Asn Ala Ala Arg Lys 
        355                 360                 365             


Ala Ala Ile Ala Ala Ser Ser Ser Phe Leu Ser Thr Ala Val Thr Phe 
    370                 375                 380                 


Pro Ser Val Gly Ser Asn Thr Leu Ala Ala Ser Lys Tyr Pro Leu Leu 
385                 390                 395                 400 


Ser Leu Leu Thr Asn Val Gly Ala Ser Gly Met Pro Val Trp Asp Val 
                405                 410                 415     


Ser Ser Gly Thr Gly Tyr Asp Glu Gly Thr Glu Leu Ile Asp Ala Leu 
            420                 425                 430         


Thr Cys Thr Thr Tyr Thr Ala Gly Ser Ser Gly Ser Val Ser Val Thr 
        435                 440                 445             


Gly Ser Ser Gly Asp Pro Val Ile Leu Leu Pro Thr Ser Ala Tyr Asn 
    450                 455                 460                 


Ala Ser Tyr Cys Ser Glu Leu Thr Gly Thr Asp Ser Thr Gly Ser Ser 
465                 470                 475                 480 


Asp Thr Val Ser Val Thr Phe Glu Val Glu Tyr Asn Thr Thr Tyr Gly 
                485                 490                 495     


Glu Asn Leu Tyr Leu Thr Gly Ser Val Ser Glu Leu Val Asp Trp Ser 
            500                 505                 510         


Val Asp Asp Ala Leu Leu Met Ser Ser Ala Asp Tyr Pro Thr Trp Ser 
        515                 520                 525             


Leu Thr Val Asp Leu Pro Pro Ser Thr Ala Ile Gln Tyr Lys Tyr Leu 
    530                 535                 540                 


Thr Lys Tyr Asn Gly Asp Val Thr Trp Glu Asp Asp Pro Asn Asn Glu 
545                 550                 555                 560 


Leu Thr Thr Pro Ala Ser Gly Ser Val Thr Gln Ser Asp Ser Trp His 
                565                 570                 575     


<210>  2
<211>  593
<212>  PRT
<213>  Fomitopsis pinicola

<400>  2

Met Trp Gly Ser Leu Leu Ala Ala Ser Ala Leu Val Ala Ser Ala Leu 
1               5                   10                  15      


Ala Ala Thr Thr Ala Glu Trp Gln Gln Arg Ser Ile Tyr Gln Leu Val 
            20                  25                  30          


Thr Asp Arg Phe Ala Thr Ser Asp Gly Ser Ser Pro Ala Cys Asp Thr 
        35                  40                  45              


Gly Asp Arg Val Tyr Cys Gly Gly Ser Trp Gln Gly Val Ile Asn Lys 
    50                  55                  60                  


Leu Asp Tyr Ile Gln Tyr Met Gly Phe Asp Ala Ile Trp Ile Ser Pro 
65                  70                  75                  80  


Val Val Lys Asn Leu Glu Gly Ser Thr Gly Asp Gly Tyr Ser Tyr His 
                85                  90                  95      


Gly Tyr Trp Ala Val Asp Gln Asn Ser Val Asn Glu His Phe Gly Thr 
            100                 105                 110         


Ala Asp Asp Leu Asn Ala Leu Ser Ser Ala Leu His Ala Arg Gly Met 
        115                 120                 125             


Tyr Leu Met Val Asp Val Val Val Asn His Met Ala Ala Asn Thr Leu 
    130                 135                 140                 


Pro Pro Asp Tyr Ser Thr Phe Thr Pro Phe Ser Ser Glu Ser Asp Phe 
145                 150                 155                 160 


His Thr Phe Cys Trp Ile Thr Asp Tyr Asp Asn Gln Thr Asn Val Glu 
                165                 170                 175     


Gln Cys Trp Leu Gly Asp Ser Ser Val Pro Leu Ala Asp Cys Asp Thr 
            180                 185                 190         


Glu Ala Asp Asn Val Ile Asp Phe Phe Tyr Asn Trp Ile Gly Glu Leu 
        195                 200                 205             


Arg Ala Asn Tyr Thr Val Asp Gly Phe Arg Ile Asp Thr Leu Lys His 
    210                 215                 220                 


Val Arg Gln Thr Phe Trp Pro Asp Phe Gln Thr Asn Ala Gly Val Tyr 
225                 230                 235                 240 


Ala Val Gly Glu Val Phe Asp Gly Asp Val Asn Tyr Val Ser Pro Tyr 
                245                 250                 255     


Thr Glu Val Ile Asp Gly Val Leu Asp Tyr Pro Thr Tyr Tyr Gln Leu 
            260                 265                 270         


Thr Ser Ala Phe Glu Ser Thr Ser Gly Ser Ile Gln Asn Leu Val Asp 
        275                 280                 285             


Val Ile Gln Ser Ala Gln Ser Thr Tyr Ser Thr Met Leu Phe Gln Val 
    290                 295                 300                 


Ala Thr Phe Leu Glu Asn Gln Asp Asn Pro Arg Phe Gln Ser Leu Thr 
305                 310                 315                 320 


Thr Asp Gln Gly Leu Val Lys Asn Ala Met Ala Trp Pro Phe Ile Ala 
                325                 330                 335     


Asp Gly Ile Pro Ile Leu Tyr Tyr Gly Gln Glu Gln Gly Tyr Thr Gly 
            340                 345                 350         


Gly Asn Asp Pro Asp Asn Arg Glu Ala Leu Trp Leu Ser Gly Tyr Glu 
        355                 360                 365             


Glu Asn Lys Pro Leu Val Gln His Ala Arg Ile Leu Asn Ala Ala Arg 
    370                 375                 380                 


Lys Ala Ala Ile Ala Ala Ser Ser Ser Phe Leu Ser Thr Ala Val Thr 
385                 390                 395                 400 


Phe Pro Ser Val Gly Ser Asn Thr Leu Ala Ala Ser Lys Tyr Pro Leu 
                405                 410                 415     


Leu Ser Leu Leu Thr Asn Val Gly Ala Ser Gly Met Pro Val Trp Asp 
            420                 425                 430         


Val Ser Ser Gly Thr Gly Tyr Asp Glu Gly Thr Glu Leu Ile Asp Ala 
        435                 440                 445             


Leu Thr Cys Thr Thr Tyr Thr Ala Gly Ser Ser Gly Ser Val Ser Val 
    450                 455                 460                 


Thr Gly Ser Ser Gly Asp Pro Val Ile Leu Leu Pro Thr Ser Ala Tyr 
465                 470                 475                 480 


Asn Ala Ser Tyr Cys Ser Glu Leu Thr Gly Thr Asp Ser Thr Gly Ser 
                485                 490                 495     


Ser Asp Thr Val Ser Val Thr Phe Glu Val Glu Tyr Asn Thr Thr Tyr 
            500                 505                 510         


Gly Glu Asn Leu Tyr Leu Thr Gly Ser Val Ser Glu Leu Val Asp Trp 
        515                 520                 525             


Ser Val Asp Asp Ala Leu Leu Met Ser Ser Ala Asp Tyr Pro Thr Trp 
    530                 535                 540                 


Ser Leu Thr Val Asp Leu Pro Pro Ser Thr Ala Ile Gln Tyr Lys Tyr 
545                 550                 555                 560 


Leu Thr Lys Tyr Asn Gly Asp Val Thr Trp Glu Asp Asp Pro Asn Asn 
                565                 570                 575     


Glu Leu Thr Thr Pro Ala Ser Gly Ser Val Thr Gln Ser Asp Ser Trp 
            580                 585                 590         


His 
    


<210>  3
<211>  1731
<212>  DNA
<213>  Fomitopsis pinicola

<400>  3
gcgactacgg cggaatggca gcaacgctcg atctaccagc tcgtcacgga tagattcgcg       60

acctcggacg gctcgtcgcc cgcttgcgac accggcgatc gcgtgtattg tggcgggtcg      120

tggcaggggg tcatcaataa gctggattac atccagtaca tgggcttcga cgccatctgg      180

atttcaccag tcgtgaagaa cctagaaggc agcacgggag atggctactc gtaccatgga      240

tactgggcgg tcgaccagaa ctcggtcaac gagcacttcg gcactgcaga cgatctgaat      300

gcccttagca gcgcgttgca cgcgcgcggg atgtacctga tggtcgacgt cgtcgtgaac      360

cacatggcgg ccaacacgct cccgccggac tactcgacct tcacgccgtt cagctccgag      420

tcggacttcc acaccttctg ctggatcacg gactacgaca accagacgaa cgtcgagcag      480

tgctggctcg gcgactcgag cgtgccgctc gcggactgcg acaccgaggc tgacaacgtc      540

atcgacttct tctacaactg gatcggcgag ctccgcgcga actacactgt ggatggtttc      600

aggatcgaca cgctgaagca tgtcaggcag acgttctggc cagatttcca gaccaacgcg      660

ggcgtgtacg ccgtcggtga ggtctttgac ggcgacgtga actatgtttc gccctacact      720

gaggttatcg acggagtgtt ggactacccg acgtactatc agctcacctc cgccttcgag      780

tccaccagcg gctcaattca gaacctcgtc gacgtgattc agtctgcgca gtccacttac      840

tccacgatgc tcttccaagt cgcgacgttc ctcgagaacc aggacaaccc gcggttccag      900

agtcttacca ctgatcaagg cctagtgaag aacgcgatgg cgtggccgtt tatcgcggat      960

ggcatcccca ttctttacta cggtcaggag caaggctaca ctggcggcaa cgaccccgat     1020

aaccgtgaag cgctgtggct gtccggatac gaggaaaaca agcctctcgt gcagcacgcc     1080

cgcatcctca acgctgcccg caaggccgcc atcgccgcca gcagcagctt cctctccacc     1140

gccgtgacct tcccgtcggt gggcagcaac acgctcgccg cgtccaaata cccgctgctc     1200

tcgctcctga ccaacgtagg cgcaagcggc atgcccgttt gggacgtctc ctcgggaacg     1260

ggatacgacg agggcacgga gctgattgat gcgctcacgt gcacgacgta caccgctggg     1320

agcagcggca gcgtgagcgt cacgggcagt agcggggacc ccgtcatcct gctcccgacg     1380

agcgcataca acgcgtcgta ctgcagcgag ctgacgggca cggactcgac cggcagctcg     1440

gacacggtgt cggtgacgtt cgaggtggag tacaacacga cgtacggcga gaatttgtat     1500

ctcaccggct ccgtctctga gctcgtggac tggtccgtcg atgacgcact ccttatgtcg     1560

tccgccgact acccgacctg gagcctgacg gtggacctcc ccccgagcac ggcgatccag     1620

tacaagtatc tgacgaagta caatggcgac gtcacgtggg aggacgaccc caacaacgag     1680

ctcacgacgc ctgcgagcgg ctccgtcacg cagagcgaca gctggcattg a              1731


<210>  4
<211>  1782
<212>  DNA
<213>  Fomitopsis pinicola

<400>  4
atgtggggca gccttctcgc agcctctgcc ctcgtggctt ccgcgcttgc tgcgactacg       60

gcggaatggc agcaacgctc gatctaccag ctcgtcacgg atagattcgc gacctcggac      120

ggctcgtcgc ccgcttgcga caccggcgat cgcgtgtatt gtggcgggtc gtggcagggg      180

gtcatcaata agctggatta catccagtac atgggcttcg acgccatctg gatttcacca      240

gtcgtgaaga acctagaagg cagcacggga gatggctact cgtaccatgg atactgggcg      300

gtcgaccaga actcggtcaa cgagcacttc ggcactgcag acgatctgaa tgcccttagc      360

agcgcgttgc acgcgcgcgg gatgtacctg atggtcgacg tcgtcgtgaa ccacatggcg      420

gccaacacgc tcccgccgga ctactcgacc ttcacgccgt tcagctccga gtcggacttc      480

cacaccttct gctggatcac ggactacgac aaccagacga acgtcgagca gtgctggctc      540

ggcgactcga gcgtgccgct cgcggactgc gacaccgagg ctgacaacgt catcgacttc      600

ttctacaact ggatcggcga gctccgcgcg aactacactg tggatggttt caggatcgac      660

acgctgaagc atgtcaggca gacgttctgg ccagatttcc agaccaacgc gggcgtgtac      720

gccgtcggtg aggtctttga cggcgacgtg aactatgttt cgccctacac tgaggttatc      780

gacggagtgt tggactaccc gacgtactat cagctcacct ccgccttcga gtccaccagc      840

ggctcaattc agaacctcgt cgacgtgatt cagtctgcgc agtccactta ctccacgatg      900

ctcttccaag tcgcgacgtt cctcgagaac caggacaacc cgcggttcca gagtcttacc      960

actgatcaag gcctagtgaa gaacgcgatg gcgtggccgt ttatcgcgga tggcatcccc     1020

attctttact acggtcagga gcaaggctac actggcggca acgaccccga taaccgtgaa     1080

gcgctgtggc tgtccggata cgaggaaaac aagcctctcg tgcagcacgc ccgcatcctc     1140

aacgctgccc gcaaggccgc catcgccgcc agcagcagct tcctctccac cgccgtgacc     1200

ttcccgtcgg tgggcagcaa cacgctcgcc gcgtccaaat acccgctgct ctcgctcctg     1260

accaacgtag gcgcaagcgg catgcccgtt tgggacgtct cctcgggaac gggatacgac     1320

gagggcacgg agctgattga tgcgctcacg tgcacgacgt acaccgctgg gagcagcggc     1380

agcgtgagcg tcacgggcag tagcggggac cccgtcatcc tgctcccgac gagcgcatac     1440

aacgcgtcgt actgcagcga gctgacgggc acggactcga ccggcagctc ggacacggtg     1500

tcggtgacgt tcgaggtgga gtacaacacg acgtacggcg agaatttgta tctcaccggc     1560

tccgtctctg agctcgtgga ctggtccgtc gatgacgcac tccttatgtc gtccgccgac     1620

tacccgacct ggagcctgac ggtggacctc cccccgagca cggcgatcca gtacaagtat     1680

ctgacgaagt acaatggcga cgtcacgtgg gaggacgacc ccaacaacga gctcacgacg     1740

cctgcgagcg gctccgtcac gcagagcgac agctggcatt ga                        1782


