                         SEQUENCE LISTING

<110>  Hangzhou New Horizon Health Technology Co. Ltd.
       Li, Hui
       Li, Cunyao
       Zheng, Weixian
       Yang, Jiao
       Liu, Gang
       Lv, Ning
       Chen, Yiyou
 
<120>  THE KIT FOR SCREENING COLORECTAL CANCER AND ADVANCED ADENOMA AND 
       ITS APPLICATION

<130>  NEWH-017/01WO 333709-2028

<150>  CN 201810502387.9
<151>  2018-05-23

<150>  CN 201810502359.7
<151>  2018-05-23

<160>  70    

<170>  PatentIn version 3.5

<210>  1
<211>  271
<212>  DNA
<213>  Homo sapiens


<220>
<221>  modified_base
<222>  (12)..(12)
<223>  methylated

<220>
<221>  modified_base
<222>  (33)..(33)
<223>  methylated

<220>
<221>  modified_base
<222>  (69)..(69)
<223>  methylated

<220>
<221>  modified_base
<222>  (80)..(80)
<223>  methylated

<220>
<221>  modified_base
<222>  (83)..(83)
<223>  methylated

<220>
<221>  modified_base
<222>  (85)..(85)
<223>  methylated

<220>
<221>  modified_base
<222>  (106)..(106)
<223>  methylated

<220>
<221>  modified_base
<222>  (120)..(120)
<223>  methylated

<220>
<221>  modified_base
<222>  (123)..(123)
<223>  methylated

<220>
<221>  modified_base
<222>  (127)..(127)
<223>  methylated

<220>
<221>  modified_base
<222>  (132)..(132)
<223>  methylated

<220>
<221>  modified_base
<222>  (139)..(139)
<223>  methylated

<220>
<221>  modified_base
<222>  (141)..(141)
<223>  methylated

<220>
<221>  modified_base
<222>  (152)..(152)
<223>  methylated

<220>
<221>  modified_base
<222>  (154)..(154)
<223>  methylated

<220>
<221>  modified_base
<222>  (189)..(189)
<223>  methylated

<220>
<221>  modified_base
<222>  (200)..(200)
<223>  methylated

<220>
<221>  modified_base
<222>  (219)..(219)
<223>  methylated

<220>
<221>  modified_base
<222>  (227)..(227)
<223>  methylated

<220>
<221>  modified_base
<222>  (236)..(236)
<223>  methylated

<220>
<221>  modified_base
<222>  (240)..(240)
<223>  methylated

<220>
<221>  modified_base
<222>  (242)..(242)
<223>  methylated

<220>
<221>  modified_base
<222>  (247)..(247)
<223>  methylated

<220>
<221>  modified_base
<222>  (254)..(254)
<223>  methylated

<220>
<221>  modified_base
<222>  (258)..(258)
<223>  methylated

<220>
<221>  modified_base
<222>  (264)..(264)
<223>  methylated

<400>  1
gttagtttgg tcgggtgttt ttaaaaataa agcgaggagg gaaggtatag atagattttg       60

aaaatattcg ggttatatac gtcgcgattt atagtttttt tttagcgttg gagtggagac      120

ggcgttcgta gcgttttgcg cgggtgaggt tcgcgtagtt gttggggaag agtttatttg      180

ttaggttgcg ttgggttagc gtagtaagtg gggttggtcg ttatttcgtt gtattcggtc      240

gcgtttcggg tttcgtgcgt tttcgtttta g                                     271


<210>  2
<211>  236
<212>  DNA
<213>  Homo sapiens


<220>
<221>  modified_base
<222>  (9)..(9)
<223>  methylated

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  methylated

<220>
<221>  modified_base
<222>  (18)..(18)
<223>  methylated

<220>
<221>  modified_base
<222>  (20)..(20)
<223>  methylated

<220>
<221>  modified_base
<222>  (25)..(25)
<223>  methylated

<220>
<221>  modified_base
<222>  (29)..(29)
<223>  methylated

<220>
<221>  modified_base
<222>  (48)..(48)
<223>  methylated

<220>
<221>  modified_base
<222>  (50)..(50)
<223>  methylated

<220>
<221>  modified_base
<222>  (53)..(53)
<223>  methylated

<220>
<221>  modified_base
<222>  (55)..(55)
<223>  methylated

<220>
<221>  modified_base
<222>  (62)..(62)
<223>  methylated

<220>
<221>  modified_base
<222>  (66)..(66)
<223>  methylated

<220>
<221>  modified_base
<222>  (74)..(74)
<223>  methylated

<220>
<221>  modified_base
<222>  (78)..(78)
<223>  methylated

<220>
<221>  modified_base
<222>  (85)..(85)
<223>  methylated

<220>
<221>  modified_base
<222>  (98)..(98)
<223>  methylated

<220>
<221>  modified_base
<222>  (100)..(100)
<223>  methylated

<220>
<221>  modified_base
<222>  (109)..(109)
<223>  methylated

<220>
<221>  modified_base
<222>  (118)..(118)
<223>  methylated

<220>
<221>  modified_base
<222>  (120)..(120)
<223>  methylated

<220>
<221>  modified_base
<222>  (122)..(122)
<223>  methylated

<220>
<221>  modified_base
<222>  (130)..(130)
<223>  methylated

<220>
<221>  modified_base
<222>  (132)..(132)
<223>  methylated

<220>
<221>  modified_base
<222>  (137)..(137)
<223>  methylated

<220>
<221>  modified_base
<222>  (141)..(141)
<223>  methylated

<220>
<221>  modified_base
<222>  (159)..(159)
<223>  methylated

<220>
<221>  modified_base
<222>  (165)..(165)
<223>  methylated

<220>
<221>  modified_base
<222>  (170)..(170)
<223>  methylated

<220>
<221>  modified_base
<222>  (172)..(172)
<223>  methylated

<220>
<221>  modified_base
<222>  (177)..(177)
<223>  methylated

<220>
<221>  modified_base
<222>  (181)..(181)
<223>  methylated

<220>
<221>  modified_base
<222>  (191)..(191)
<223>  methylated

<220>
<221>  modified_base
<222>  (202)..(202)
<223>  methylated

<220>
<221>  modified_base
<222>  (204)..(204)
<223>  methylated

<220>
<221>  modified_base
<222>  (210)..(210)
<223>  methylated

<220>
<221>  modified_base
<222>  (216)..(216)
<223>  methylated

<220>
<221>  modified_base
<222>  (218)..(218)
<223>  methylated

<220>
<221>  modified_base
<222>  (221)..(221)
<223>  methylated

<220>
<221>  modified_base
<222>  (226)..(226)
<223>  methylated

<400>  2
tgagaagtcg gcgggggcgc ggatcgatcg gggtgttttt taggtttcgc gtcgcggttt       60

tcgttcgttt tttcgttcgt ttatcgggta ttttagtcgc gtagaaggcg gaagttacgc      120

gcgagggatc gcggttcgtt cgggattagt tttaggttcg gtatcgtttc gcgggtcgag      180

cgtttatatt cgttaaattt acgcgggtac gttttcgcgg cgtatcgttt ttagtt          236


<210>  3
<211>  29
<212>  DNA
<213>  Homo sapiens

<400>  3
tttgaaaata ttcgggttat atacgtcgc                                         29


<210>  4
<211>  28
<212>  DNA
<213>  Homo sapiens

<400>  4
ataaactctt ccccaacaac tacgcgaa                                          28


<210>  5
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  5
agcgttggag tggagacggc gttcg                                             25


<210>  6
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  6
atcgatcggg gtgtttttta ggtttc                                            26


<210>  7
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  7
ccttctacgc gactaaaata cccgat                                            26


<210>  8
<211>  31
<212>  DNA
<213>  Homo sapiens

<400>  8
cgtcgcggtt ttcgttcgtt ttttcgttcg t                                      31


<210>  9
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  9
aatattcggg ttatatacgt cgcga                                             25


<210>  10
<211>  28
<212>  DNA
<213>  Homo sapiens

<400>  10
gcaacctaac aaataaactc ttccccaa                                          28


<210>  11
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  11
tggagtggag acggcgttcg tagcgt                                            26


<210>  12
<211>  17
<212>  DNA
<213>  Homo sapiens

<400>  12
gcgggtgaga agtcggc                                                      17


<210>  13
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  13
gtaacttccg ccttctacgc                                                   20


<210>  14
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  14
taggtttcgc gtcgcggttt tcgtt                                             25


<210>  15
<211>  27
<212>  DNA
<213>  Homo sapiens

<400>  15
aatattcggg ttatatacgt cgcgatt                                           27


<210>  16
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  16
acttactacg ctaacccaac g                                                 21


<210>  17
<211>  28
<212>  DNA
<213>  Homo sapiens

<400>  17
tagcgttgga gtggagacgg cgttcgta                                          28


<210>  18
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  18
cggttttcgt tcgttttttc g                                                 21


<210>  19
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  19
aacctaaaac taatcccgaa cgaacc                                            26


<210>  20
<211>  28
<212>  DNA
<213>  Homo sapiens

<400>  20
tcgtttatcg ggtattttag tcgcgtag                                          28


<210>  21
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  21
gagtggagac ggcgttcgta                                                   20


<210>  22
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  22
ccacttacta cgctaaccca acg                                               23


<210>  23
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  23
cgggtgaggt tcgcgtagtt gttggg                                            26


<210>  24
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  24
gggtgttttt taggtttcgc gtc                                               23


<210>  25
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  25
cgtaacttcc gccttctacg c                                                 21


<210>  26
<211>  40
<212>  DNA
<213>  Homo sapiens

<400>  26
acgcgactaa aatacccgat aaacgaacga aaaaacgaac                             40


<210>  27
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  27
ttaggttgcg ttgggttagc g                                                 21


<210>  28
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  28
actccgaaaa cgcaaaaaac cg                                                22


<210>  29
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  29
attcggtcgc gtttcgggtt tcgtgc                                            26


<210>  30
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  30
gacccgcgaa acgataccg                                                    19


<210>  31
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  31
tattcgggtt atatacgtcg c                                                 21


<210>  32
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  32
cttactacgc taacccaacg                                                   20


<210>  33
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  33
cccaacaact acgcgaacct cacccg                                            26


<210>  34
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  34
tcgcgcgtaa cttccgcctt                                                   20


<210>  35
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  35
aacttgtggt agttggaggt ga                                                22


<210>  36
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  36
aacttgtggt agttggagct gggga                                             25


<210>  37
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  37
aacttgtggt agttggagtt gt                                                22


<210>  38
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  38
aaacttgtgg tagttggggc tt                                                22


<210>  39
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  39
aaacttgtgg tagttggtgc ta                                                22


<210>  40
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  40
aacttgtggt agttggagca gc                                                22


<210>  41
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  41
aaacttgtgg tagttggagc tc                                                22


<210>  42
<211>  24
<212>  DNA
<213>  Homo sapiens

<400>  42
gaatggtcct gcaccagtaa tatg                                              24


<210>  43
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  43
agggcttctt gtcctttcct t                                                 21


<210>  44
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  44
cgtgctcgat ggggtacttc                                                   20


<210>  45
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  45
aggcaagagt gccttgacga tacagc                                            26


<210>  46
<211>  27
<212>  DNA
<213>  Homo sapiens

<400>  46
cgtgatggtg ggcatgggtc agaagga                                           27


<210>  47
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  47
agtttggtgt aagttaagag                                                   20


<210>  48
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  48
ctaactctat tttaaacrcc a                                                 21


<210>  49
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  49
gttttaattt ttggaaaagg taa                                               23


<210>  50
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  50
acctaacaaa taaactcttc c                                                 21


<210>  51
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  51
gaaggtatag atagattttg aa                                                22


<210>  52
<211>  24
<212>  DNA
<213>  Homo sapiens

<400>  52
cacctaacac aactttacra aact                                              24


<210>  53
<211>  26
<212>  DNA
<213>  Homo sapiens

<400>  53
gtatttagtt atggttgggg ygagta                                            26


<210>  54
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  54
ctcacctact actaccgccc r                                                 21


<210>  55
<211>  25
<212>  DNA
<213>  Homo sapiens

<400>  55
aggtttttga gtttttggtt ttttt                                             25


<210>  56
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  56
ccctccaaac cccctataac                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  57
ggatggggat gtttttgtag                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  58
rgrgaaacct aaaaaacacc                                                   20


<210>  59
<211>  18
<212>  DNA
<213>  Homo sapiens

<400>  59
gyggagyggg tgagaagt                                                     18


<210>  60
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  60
craacaacca aaaacccctc                                                   20


<210>  61
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  61
gttygttygg gattagtttt agg                                               23


<210>  62
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  62
crcaaacraa aaacraaac                                                    19


<210>  63
<211>  18
<212>  DNA
<213>  Homo sapiens

<400>  63
gyggygtttt ygtttttg                                                     18


<210>  64
<211>  20
<212>  DNA
<213>  Homo sapiens

<400>  64
cracractaa aaatccccaa                                                   20


<210>  65
<211>  271
<212>  DNA
<213>  Homo sapiens


<220>
<221>  modified_base
<222>  (12)..(12)
<223>  methylated

<220>
<221>  modified_base
<222>  (33)..(33)
<223>  methylated

<220>
<221>  modified_base
<222>  (69)..(69)
<223>  methylated

<220>
<221>  modified_base
<222>  (80)..(80)
<223>  methylated

<220>
<221>  modified_base
<222>  (83)..(83)
<223>  methylated

<220>
<221>  modified_base
<222>  (85)..(85)
<223>  methylated

<220>
<221>  modified_base
<222>  (106)..(106)
<223>  methylated

<220>
<221>  modified_base
<222>  (120)..(120)
<223>  methylated

<220>
<221>  modified_base
<222>  (123)..(123)
<223>  methylated

<220>
<221>  modified_base
<222>  (132)..(132)
<223>  methylated

<220>
<221>  modified_base
<222>  (139)..(139)
<223>  methylated

<220>
<221>  modified_base
<222>  (141)..(141)
<223>  methylated

<220>
<221>  modified_base
<222>  (152)..(152)
<223>  methylated

<220>
<221>  modified_base
<222>  (154)..(154)
<223>  methylated

<220>
<221>  modified_base
<222>  (189)..(189)
<223>  methylated

<220>
<221>  modified_base
<222>  (200)..(200)
<223>  methylated

<220>
<221>  modified_base
<222>  (219)..(219)
<223>  methylated

<220>
<221>  modified_base
<222>  (227)..(227)
<223>  methylated

<220>
<221>  modified_base
<222>  (240)..(240)
<223>  methylated

<220>
<221>  modified_base
<222>  (242)..(242)
<223>  methylated

<220>
<221>  modified_base
<222>  (247)..(247)
<223>  methylated

<220>
<221>  modified_base
<222>  (254)..(254)
<223>  methylated

<220>
<221>  modified_base
<222>  (258)..(258)
<223>  methylated

<220>
<221>  modified_base
<222>  (264)..(264)
<223>  methylated

<400>  65
gccagtttgg ccgggtgttc ccaaaaataa agcgaggagg gaaggtacag acagatcttg       60

aaaacacccg ggccacacac gccgcgacct acagctcttt ctcagcgttg gagtggagac      120

ggcgcccgca gcgccctgcg cgggtgaggt ccgcgcagct gctggggaag agcccacctg      180

tcaggctgcg ctgggtcagc gcagcaagtg gggctggccg ctatctcgct gcacccggcc      240

gcgtcccggg ctccgtgcgc cctcgcccca g                                     271


<210>  66
<211>  236
<212>  DNA
<213>  Homo sapiens


<220>
<221>  modified_base
<222>  (9)..(9)
<223>  methylated

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  methylated

<220>
<221>  modified_base
<222>  (18)..(18)
<223>  methylated

<220>
<221>  modified_base
<222>  (20)..(20)
<223>  methylated

<220>
<221>  modified_base
<222>  (25)..(25)
<223>  methylated

<220>
<221>  modified_base
<222>  (29)..(29)
<223>  methylated

<220>
<221>  modified_base
<222>  (48)..(48)
<223>  methylated

<220>
<221>  modified_base
<222>  (50)..(50)
<223>  methylated

<220>
<221>  modified_base
<222>  (53)..(53)
<223>  methylated

<220>
<221>  modified_base
<222>  (55)..(55)
<223>  methylated

<220>
<221>  modified_base
<222>  (62)..(62)
<223>  methylated

<220>
<221>  modified_base
<222>  (66)..(66)
<223>  methylated

<220>
<221>  modified_base
<222>  (74)..(74)
<223>  methylated

<220>
<221>  modified_base
<222>  (78)..(78)
<223>  methylated

<220>
<221>  modified_base
<222>  (85)..(85)
<223>  methylated

<220>
<221>  modified_base
<222>  (98)..(98)
<223>  methylated

<220>
<221>  modified_base
<222>  (100)..(100)
<223>  methylated

<220>
<221>  modified_base
<222>  (109)..(109)
<223>  methylated

<220>
<221>  modified_base
<222>  (118)..(118)
<223>  methylated

<220>
<221>  modified_base
<222>  (120)..(120)
<223>  methylated

<220>
<221>  modified_base
<222>  (122)..(122)
<223>  methylated

<220>
<221>  modified_base
<222>  (130)..(130)
<223>  methylated

<220>
<221>  modified_base
<222>  (132)..(132)
<223>  methylated

<220>
<221>  modified_base
<222>  (137)..(137)
<223>  methylated

<220>
<221>  modified_base
<222>  (141)..(141)
<223>  methylated

<220>
<221>  modified_base
<222>  (159)..(159)
<223>  methylated

<220>
<221>  modified_base
<222>  (165)..(165)
<223>  methylated

<220>
<221>  modified_base
<222>  (170)..(170)
<223>  methylated

<220>
<221>  modified_base
<222>  (172)..(172)
<223>  methylated

<220>
<221>  modified_base
<222>  (177)..(177)
<223>  methylated

<220>
<221>  modified_base
<222>  (181)..(181)
<223>  methylated

<220>
<221>  modified_base
<222>  (191)..(191)
<223>  methylated

<220>
<221>  modified_base
<222>  (202)..(202)
<223>  methylated

<220>
<221>  modified_base
<222>  (204)..(204)
<223>  methylated

<220>
<221>  modified_base
<222>  (210)..(210)
<223>  methylated

<220>
<221>  modified_base
<222>  (216)..(216)
<223>  methylated

<220>
<221>  modified_base
<222>  (218)..(218)
<223>  methylated

<220>
<221>  modified_base
<222>  (221)..(221)
<223>  methylated

<220>
<221>  modified_base
<222>  (226)..(226)
<223>  methylated

<400>  66
tgagaagtcg gcgggggcgc ggatcgaccg gggtgtcccc caggctccgc gtcgcggtcc       60

ccgctcgccc tcccgcccgc ccaccgggca ccccagccgc gcagaaggcg gaagccacgc      120

gcgagggacc gcggtccgtc cgggactagc cccaggcccg gcaccgcccc gcgggccgag      180

cgcccacacc cgccaaaccc acgcgggcac gcccccgcgg cgcaccgccc ccagcc          236


<210>  67
<211>  271
<212>  DNA
<213>  Homo sapiens

<400>  67
gttagtttgg tcgggtgttt ttaaaaataa agcgaggagg gaaggtatag atagattttg       60

aaaatattcg ggttatatac gtcgcgattt atagtttttt tttagcgttg gagtggagac      120

ggcgttcgta gcgttttgcg cgggtgaggt tcgcgtagtt gttggggaag agtttatttg      180

ttaggttgcg ttgggttagc gtagtaagtg gggttggtcg ttatttcgtt gtattcggtc      240

gcgtttcggg tttcgtgcgt tttcgtttta g                                     271


<210>  68
<211>  271
<212>  DNA
<213>  Homo sapiens

<400>  68
gttagtttgg ttgggtgttt ttaaaaataa agtgaggagg gaaggtatag atagattttg       60

aaaatatttg ggttatatat gttgtgattt atagtttttt tttagtgttg gagtggagat      120

ggtgtttgta gtgttttgtg tgggtgaggt ttgtgtagtt gttggggaag agtttatttg      180

ttaggttgtg ttgggttagt gtagtaagtg gggttggttg ttattttgtt gtatttggtt      240

gtgttttggg ttttgtgtgt ttttgtttta g                                     271


<210>  69
<211>  236
<212>  DNA
<213>  Homo sapiens

<400>  69
tgagaagtcg gcgggggcgc ggatcgatcg gggtgttttt taggtttcgc gtcgcggttt       60

tcgttcgttt tttcgttcgt ttatcgggta ttttagtcgc gtagaaggcg gaagttacgc      120

gcgagggatc gcggttcgtt cgggattagt tttaggttcg gtatcgtttc gcgggtcgag      180

cgtttatatt cgttaaattt acgcgggtac gttttcgcgg cgtatcgttt ttagtt          236


<210>  70
<211>  236
<212>  DNA
<213>  Homo sapiens

<400>  70
tgagaagttg gtgggggtgt ggattgattg gggtgttttt taggttttgt gttgtggttt       60

ttgtttgttt ttttgtttgt ttattgggta ttttagttgt gtagaaggtg gaagttatgt      120

gtgagggatt gtggtttgtt tgggattagt tttaggtttg gtattgtttt gtgggttgag      180

tgtttatatt tgttaaattt atgtgggtat gtttttgtgg tgtattgttt ttagtt          236



