                         SEQUENCE LISTING

<110>  AGAINCHANCE CORPORATION LIMITED
       LIOU, Terry
 
<120>  BISPECIFIC T CELL ENGAGER AND USES THEREOF

<130>  NONE

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  531
<212>  DNA
<213>  Homo sapiens


<220>
<221>  CDS
<222>  (1)..(531)

<400>  1
agg aca gag gac ctg cca aag gcc gtg gtg ttt ctg gag ccc cag tgg         48
Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro Gln Trp           
1               5                   10                  15                

tac cgc gtg ctg gag aag gac tcc gtg aca ctg aag tgc cag ggc gcc         96
Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln Gly Ala           
            20                  25                  30                    

tat agc cct gag gat aac tcc acc cag tgg ttc cac aat gag agc ctg        144
Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu Ser Leu           
        35                  40                  45                        

atc agc tcc cag gcc tct agc tac ttt atc gac gca gca acc gtg gac        192
Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr Val Asp           
    50                  55                  60                            

gat tcc gga gag tat cgg tgc cag acc aac ctg agc aca ctg tcc gat        240
Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu Ser Asp           
65                  70                  75                  80            

cca gtg cag ctg gag gtg cac atc gga tgg ctg ctg ctg cag gca cct        288
Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln Ala Pro           
                85                  90                  95                

aga tgg gtg ttc aag gag gag gac ccc atc cac ctg cgc tgt cac agc        336
Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys His Ser           
            100                 105                 110                   

tgg aag aat acc gcc ctg cac aag gtg aca tac ctg cag aac ggc aag        384
Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn Gly Lys           
        115                 120                 125                       

ggc cgg aag tac ttc cac cac aat tct gac ttt tat atc ccc aag gcc        432
Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile Pro Lys Ala           
    130                 135                 140                           

aca ctg aag gat agc ggc tcc tat ttt tgc aga ggc ctg gtg ggc agc        480
Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Val Gly Ser           
145                 150                 155                 160           

aag aac gtg tcc tct gag acc gtg aat atc acc atc aca cag gga ctg        528
Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln Gly Leu           
                165                 170                 175               

gca                                                                    531
Ala                                                                       
                                                                          


<210>  2
<211>  177
<212>  PRT
<213>  Homo sapiens

<400>  2

Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu Pro Gln Trp 
1               5                   10                  15      


Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys Gln Gly Ala 
            20                  25                  30          


Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn Glu Ser Leu 
        35                  40                  45              


Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala Thr Val Asp 
    50                  55                  60                  


Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr Leu Ser Asp 
65                  70                  75                  80  


Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu Gln Ala Pro 
                85                  90                  95      


Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg Cys His Ser 
            100                 105                 110         


Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln Asn Gly Lys 
        115                 120                 125             


Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile Pro Lys Ala 
    130                 135                 140                 


Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu Val Gly Ser 
145                 150                 155                 160 


Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr Gln Gly Leu 
                165                 170                 175     


Ala 
    


<210>  3
<211>  732
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  scFv


<220>
<221>  CDS
<222>  (1)..(732)

<400>  3
cag gtg cag ctg cag cag agc gga gca gag ctg gca agg cct gga gcc         48
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala           
1               5                   10                  15                

tcc gtg aag atg tct tgt aag gcc agc ggc tac acc ttc aca cgg tat         96
Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr           
            20                  25                  30                    

aca atg cac tgg gtg aag cag aga cca gga cag gga ctg gag tgg atc        144
Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile           
        35                  40                  45                        

gga tac atc aac cct tcc cgc ggc tac acc aac tat aat cag aag ttt        192
Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe           
    50                  55                  60                            

aag gac aag gcc acc ctg acc aca gat aag agc tcc tct aca gcc tac        240
Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr           
65                  70                  75                  80            

atg cag ctg agc tcc ctg acc tct gag gac agc gcc gtg tac tat tgc        288
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys           
                85                  90                  95                

gcc aga tac tat gac gat cac tac tgt ctg gat tat tgg ggc cag ggc        336
Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly           
            100                 105                 110                   

acc aca ctg aca gtg tct agc gtg gag gga ggc tcc gga ggc tct gga        384
Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly           
        115                 120                 125                       

ggc agc ggc ggc tcc gga gga gtg gac cag atc gtg ctg acc cag tcc        432
Gly Ser Gly Gly Ser Gly Gly Val Asp Gln Ile Val Leu Thr Gln Ser           
    130                 135                 140                           

cca gca atc atg tct gcc agc cct gga gag aag gtg acc atg aca tgc        480
Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys           
145                 150                 155                 160           

tct gcc tcc tct agc gtg agc tac atg aat tgg tat cag cag aag tct        528
Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser           
                165                 170                 175               

ggc aca agc cca aag cgg tgg atc tac gac acc tcc aag ctg gca tct        576
Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser           
            180                 185                 190                   

gga gtg cca gca cac ttc aga ggc tct ggc agc ggc acc tcc tat tct        624
Gly Val Pro Ala His Phe Arg Gly Ser Gly Ser Gly Thr Ser Tyr Ser           
        195                 200                 205                       

ctg aca atc tcc gga atg gag gca gag gat gca gca acc tac tat tgt        672
Leu Thr Ile Ser Gly Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys           
    210                 215                 220                           

cag cag tgg tcc tct aac ccc ttc acc ttt ggc tct ggc aca aag ctg        720
Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu           
225                 230                 235                 240           

gag atc aat aga                                                        732
Glu Ile Asn Arg                                                           
                                                                          


<210>  4
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Arg Tyr 
            20                  25                  30          


Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser Gly Gly Ser Gly 
        115                 120                 125             


Gly Ser Gly Gly Ser Gly Gly Val Asp Gln Ile Val Leu Thr Gln Ser 
    130                 135                 140                 


Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys 
145                 150                 155                 160 


Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr Gln Gln Lys Ser 
                165                 170                 175     


Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser Lys Leu Ala Ser 
            180                 185                 190         


Gly Val Pro Ala His Phe Arg Gly Ser Gly Ser Gly Thr Ser Tyr Ser 
        195                 200                 205             


Leu Thr Ile Ser Gly Met Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys 
    210                 215                 220                 


Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu 
225                 230                 235                 240 


Glu Ile Asn Arg 
                


<210>  5
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  secretion signal


<220>
<221>  CDS
<222>  (1)..(57)

<400>  5
atg gag tgc agc tgc gtg atg ctg ttc ctg ctg tcc gga acc gca ggc         48
Met Glu Cys Ser Cys Val Met Leu Phe Leu Leu Ser Gly Thr Ala Gly           
1               5                   10                  15                

gtg ctg tct                                                             57
Val Leu Ser                                                               
                                                                          


<210>  6
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Glu Cys Ser Cys Val Met Leu Phe Leu Leu Ser Gly Thr Ala Gly 
1               5                   10                  15      


Val Leu Ser 
            


<210>  7
<211>  1341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion protein


<220>
<221>  CDS
<222>  (1)..(1338)

<400>  7
atg gag tgc agc tgc gtg atg ctg ttc ctg ctg tcc gga acc gca ggc         48
Met Glu Cys Ser Cys Val Met Leu Phe Leu Leu Ser Gly Thr Ala Gly           
1               5                   10                  15                

gtg ctg tct agg aca gag gac ctg cca aag gcc gtg gtg ttt ctg gag         96
Val Leu Ser Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu           
            20                  25                  30                    

ccc cag tgg tac cgc gtg ctg gag aag gac tcc gtg aca ctg aag tgc        144
Pro Gln Trp Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys           
        35                  40                  45                        

cag ggc gcc tat agc cct gag gat aac tcc acc cag tgg ttc cac aat        192
Gln Gly Ala Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn           
    50                  55                  60                            

gag agc ctg atc agc tcc cag gcc tct agc tac ttt atc gac gca gca        240
Glu Ser Leu Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala           
65                  70                  75                  80            

acc gtg gac gat tcc gga gag tat cgg tgc cag acc aac ctg agc aca        288
Thr Val Asp Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr           
                85                  90                  95                

ctg tcc gat cca gtg cag ctg gag gtg cac atc gga tgg ctg ctg ctg        336
Leu Ser Asp Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu           
            100                 105                 110                   

cag gca cct aga tgg gtg ttc aag gag gag gac ccc atc cac ctg cgc        384
Gln Ala Pro Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg           
        115                 120                 125                       

tgt cac agc tgg aag aat acc gcc ctg cac aag gtg aca tac ctg cag        432
Cys His Ser Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln           
    130                 135                 140                           

aac ggc aag ggc cgg aag tac ttc cac cac aat tct gac ttt tat atc        480
Asn Gly Lys Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile           
145                 150                 155                 160           

ccc aag gcc aca ctg aag gat agc ggc tcc tat ttt tgc aga ggc ctg        528
Pro Lys Ala Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu           
                165                 170                 175               

gtg ggc agc aag aac gtg tcc tct gag acc gtg aat atc acc atc aca        576
Val Gly Ser Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr           
            180                 185                 190                   

cag gga ctg gca cag gtg cag ctg cag cag agc gga gca gag ctg gca        624
Gln Gly Leu Ala Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala           
        195                 200                 205                       

agg cct gga gcc tcc gtg aag atg tct tgt aag gcc agc ggc tac acc        672
Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr           
    210                 215                 220                           

ttc aca cgg tat aca atg cac tgg gtg aag cag aga cca gga cag gga        720
Phe Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly           
225                 230                 235                 240           

ctg gag tgg atc gga tac atc aac cct tcc cgc ggc tac acc aac tat        768
Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr           
                245                 250                 255               

aat cag aag ttt aag gac aag gcc acc ctg acc aca gat aag agc tcc        816
Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser           
            260                 265                 270                   

tct aca gcc tac atg cag ctg agc tcc ctg acc tct gag gac agc gcc        864
Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala           
        275                 280                 285                       

gtg tac tat tgc gcc aga tac tat gac gat cac tac tgt ctg gat tat        912
Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr           
    290                 295                 300                           

tgg ggc cag ggc acc aca ctg aca gtg tct agc gtg gag gga ggc tcc        960
Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser           
305                 310                 315                 320           

gga ggc tct gga ggc agc ggc ggc tcc gga gga gtg gac cag atc gtg       1008
Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Gln Ile Val           
                325                 330                 335               

ctg acc cag tcc cca gca atc atg tct gcc agc cct gga gag aag gtg       1056
Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val           
            340                 345                 350                   

acc atg aca tgc tct gcc tcc tct agc gtg agc tac atg aat tgg tat       1104
Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr           
        355                 360                 365                       

cag cag aag tct ggc aca agc cca aag cgg tgg atc tac gac acc tcc       1152
Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser           
    370                 375                 380                           

aag ctg gca tct gga gtg cca gca cac ttc aga ggc tct ggc agc ggc       1200
Lys Leu Ala Ser Gly Val Pro Ala His Phe Arg Gly Ser Gly Ser Gly           
385                 390                 395                 400           

acc tcc tat tct ctg aca atc tcc gga atg gag gca gag gat gca gca       1248
Thr Ser Tyr Ser Leu Thr Ile Ser Gly Met Glu Ala Glu Asp Ala Ala           
                405                 410                 415               

acc tac tat tgt cag cag tgg tcc tct aac ccc ttc acc ttt ggc tct       1296
Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Ser           
            420                 425                 430                   

ggc aca aag ctg gag atc aat aga cat cac cac cac cac cac tga           1341
Gly Thr Lys Leu Glu Ile Asn Arg His His His His His His                   
        435                 440                 445                       


<210>  8
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Glu Cys Ser Cys Val Met Leu Phe Leu Leu Ser Gly Thr Ala Gly 
1               5                   10                  15      


Val Leu Ser Arg Thr Glu Asp Leu Pro Lys Ala Val Val Phe Leu Glu 
            20                  25                  30          


Pro Gln Trp Tyr Arg Val Leu Glu Lys Asp Ser Val Thr Leu Lys Cys 
        35                  40                  45              


Gln Gly Ala Tyr Ser Pro Glu Asp Asn Ser Thr Gln Trp Phe His Asn 
    50                  55                  60                  


Glu Ser Leu Ile Ser Ser Gln Ala Ser Ser Tyr Phe Ile Asp Ala Ala 
65                  70                  75                  80  


Thr Val Asp Asp Ser Gly Glu Tyr Arg Cys Gln Thr Asn Leu Ser Thr 
                85                  90                  95      


Leu Ser Asp Pro Val Gln Leu Glu Val His Ile Gly Trp Leu Leu Leu 
            100                 105                 110         


Gln Ala Pro Arg Trp Val Phe Lys Glu Glu Asp Pro Ile His Leu Arg 
        115                 120                 125             


Cys His Ser Trp Lys Asn Thr Ala Leu His Lys Val Thr Tyr Leu Gln 
    130                 135                 140                 


Asn Gly Lys Gly Arg Lys Tyr Phe His His Asn Ser Asp Phe Tyr Ile 
145                 150                 155                 160 


Pro Lys Ala Thr Leu Lys Asp Ser Gly Ser Tyr Phe Cys Arg Gly Leu 
                165                 170                 175     


Val Gly Ser Lys Asn Val Ser Ser Glu Thr Val Asn Ile Thr Ile Thr 
            180                 185                 190         


Gln Gly Leu Ala Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala 
        195                 200                 205             


Arg Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr 
    210                 215                 220                 


Phe Thr Arg Tyr Thr Met His Trp Val Lys Gln Arg Pro Gly Gln Gly 
225                 230                 235                 240 


Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Arg Gly Tyr Thr Asn Tyr 
                245                 250                 255     


Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Thr Asp Lys Ser Ser 
            260                 265                 270         


Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala 
        275                 280                 285             


Val Tyr Tyr Cys Ala Arg Tyr Tyr Asp Asp His Tyr Cys Leu Asp Tyr 
    290                 295                 300                 


Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Val Glu Gly Gly Ser 
305                 310                 315                 320 


Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Val Asp Gln Ile Val 
                325                 330                 335     


Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val 
            340                 345                 350         


Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met Asn Trp Tyr 
        355                 360                 365             


Gln Gln Lys Ser Gly Thr Ser Pro Lys Arg Trp Ile Tyr Asp Thr Ser 
    370                 375                 380                 


Lys Leu Ala Ser Gly Val Pro Ala His Phe Arg Gly Ser Gly Ser Gly 
385                 390                 395                 400 


Thr Ser Tyr Ser Leu Thr Ile Ser Gly Met Glu Ala Glu Asp Ala Ala 
                405                 410                 415     


Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Phe Thr Phe Gly Ser 
            420                 425                 430         


Gly Thr Lys Leu Glu Ile Asn Arg His His His His His His 
        435                 440                 445     


