                         SEQUENCE LISTING

<110>  THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR 
       UNIVERSITY
       Garcia, Christopher K
       Fernandes, Ricardo A
 
<120>  RECEPTOR INHIBITION BY PHOSPHATASE RECRUITMENT

<130>  78430-504001WO

<140>  Herewith
<141>  Herewith

<150>  US 62/673,049
<151>  2018-05-17

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  1611
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  antiCD45-antiPD1 using scFv from Nivolumab modified to include Cys

<220>
<221>  misc_feature
<223>  encodes the peptide sequence at SEQ ID NO 2

<400>  1
atgctactag taaatcagtc acaccaaggc ttcaataagg aacacacaag caagatggta       60

agcgctattg ttttatatgt gcttttggcg gcggcggcgc attctgcctt tgcgggatcc      120

caggttcagc tcgtagaatc tggagccgaa gtcaagaaat gcggagcatc agtaaaggtt      180

agctgcaagg cgtccggata caccttcacc aattacatca ttcattgggt gaagcaagag      240

ccgggccaag gtctggaatg gatcggatac tttaatccat acaaccacgg aacgaaatac      300

aacgaaaaat ttaaaggcag ggcaaccctc actgccgaca agtccattag taccgcgtac      360

atggagttgt ccagtttgac ctcagaggat accgctgtct actactgcgc aaggagtggc      420

ccctacgctt ggttcgatac ctggggccag ggaactactg tcacggtatc cagtggtggc      480

ggtggtagcg aaatcgtact gacccagagc cctgcgactt tatctttatc tcctggcgaa      540

agggctaccc tgtcatgtcg cgcatctcag tccgtctcat cgtacctcgc ctggtaccag      600

cagaaacccg gacaagcgcc tcgtcttttg atctatgacg cctctaaccg tgccaccggt      660

attccagctc gtttctctgg ctccggttcc ggcacagact tcactttgac gatttcttca      720

ctcgaacctg aagactttgc agtctattac tgccagcaga gttccaactg gcctcgcacc      780

ttcggccaga ctaaggtgga gattaaaaga agggcagacg cggccgccgc tggcggtggt      840

ggaagcggtg gaggaggatc tggcggtgga ggttccggtg gaggaggaac gggtggtgga      900

ggtagtcaag ttcagttggt ggaatctggc ggcggagtcg tacagccggg acgctcattg      960

agactggact gtaaggctag tggaatcaca ttcagcaact ccggaatgca ctgggttagg     1020

caagctcccg gcaaaggatt ggaatgggtc gccgtaattt ggtacgacgg atctaagagg     1080

tactacgctg acagcgtttg cggtaggttc acaatctcca gggataactc aaagaacaca     1140

ctttttctgc aaatgaactc cctcaccgcc gaggacaccg ccgtttacta ctgtgcaact     1200

aacgatgatt attggggtca gggtactctg gtcactgtca gctccggtgg cggaggatca     1260

gacatcctgt taacacagag cccggccacg ttatcgctct ctccaggcga aagggccact     1320

ttaagctgca gagcttccca aaacatagga acttcgatcc aatggtacca acaaaagccg     1380

ggtcaggcac cgagactgtt gatccgttcc agctctgaaa gcattagcgg catctcctcg     1440

aggttttcag gttccggttc aggtaccgac ttcacgctga ctatctcttc tctggagcct     1500

gaagatttcg cagtatacta ctgtcagcag agcaacactt ggccattcac cttcggacag     1560

ggtactaagc tggagatcaa agcggccgcg catcatcacc accatcacca c              1611


<210>  2
<211>  537
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  antiCD45-antiPD1 using scFv from Nivolumab modified to include Cys

<220>
<221>  misc_feature
<222>  (1)..(38)
<223>  Signal Peptide

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  Linker

<220>
<221>  misc_feature
<222>  (41)..(158)
<223>  heavy chain variable region of anti-CD45 scFv

<220>
<221>  misc_feature
<222>  (159)..(163)
<223>  Linker

<220>
<221>  misc_feature
<222>  (164)..(269)
<223>  light chain variable region of anti-PD1 scFv

<220>
<221>  misc_feature
<222>  (270)..(302)
<223>  Linker

<220>
<221>  misc_feature
<222>  (303)..(415)
<223>  heavy chain variable region of anti-PD1 scFv

<220>
<221>  misc_feature
<222>  (416)..(420)
<223>  Linker

<220>
<221>  misc_feature
<222>  (421)..(527)
<223>  light chain variable region of anti-CD45 scFv

<220>
<221>  misc_feature
<222>  (528)..(537)
<223>  His Tag

<400>  2

Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      


Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          


Ala His Ser Ala Phe Ala Gly Ser Gln Val Gln Leu Val Glu Ser Gly 
        35                  40                  45              


Ala Glu Val Lys Lys Cys Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
    50                  55                  60                  


Ser Gly Tyr Thr Phe Thr Asn Tyr Ile Ile His Trp Val Lys Gln Glu 
65                  70                  75                  80  


Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Phe Asn Pro Tyr Asn His 
                85                  90                  95      


Gly Thr Lys Tyr Asn Glu Lys Phe Lys Gly Arg Ala Thr Leu Thr Ala 
            100                 105                 110         


Asp Lys Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Thr Ser 
        115                 120                 125             


Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly Pro Tyr Ala Trp 
    130                 135                 140                 


Phe Asp Thr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu 
                165                 170                 175     


Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val 
            180                 185                 190         


Ser Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg 
        195                 200                 205             


Leu Leu Ile Tyr Asp Ala Ser Asn Arg Ala Thr Gly Ile Pro Ala Arg 
    210                 215                 220                 


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
225                 230                 235                 240 


Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Ser Ser Asn 
                245                 250                 255     


Trp Pro Arg Thr Phe Gly Gln Thr Lys Val Glu Ile Lys Arg Arg Ala 
            260                 265                 270         


Asp Ala Ala Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        275                 280                 285             


Gly Gly Gly Ser Gly Gly Gly Gly Thr Gly Gly Gly Gly Ser Gln Val 
    290                 295                 300                 


Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu 
305                 310                 315                 320 


Arg Leu Asp Cys Lys Ala Ser Gly Ile Thr Phe Ser Asn Ser Gly Met 
                325                 330                 335     


His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Val 
            340                 345                 350         


Ile Trp Tyr Asp Gly Ser Lys Arg Tyr Tyr Ala Asp Ser Val Cys Gly 
        355                 360                 365             


Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Phe Leu Gln 
    370                 375                 380                 


Met Asn Ser Leu Thr Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Thr 
385                 390                 395                 400 


Asn Asp Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly 
                405                 410                 415     


Gly Gly Gly Ser Asp Ile Leu Leu Thr Gln Ser Pro Ala Thr Leu Ser 
            420                 425                 430         


Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Asn 
        435                 440                 445             


Ile Gly Thr Ser Ile Gln Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro 
    450                 455                 460                 


Arg Leu Leu Ile Arg Ser Ser Ser Glu Ser Ile Ser Gly Ile Ser Ser 
465                 470                 475                 480 


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                485                 490                 495     


Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Ser Asn 
            500                 505                 510         


Thr Trp Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Ala 
        515                 520                 525             


Ala Ala His His His His His His His 
    530                 535         


<210>  3
<211>  1647
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  antiCD45-antiPD1 using scFv from 1B8 

<220>
<221>  misc_feature
<223>  encodes the peptide sequence at SEQ ID NO 4

<400>  3
atgctactag taaatcagtc acaccaaggc ttcaataagg aacacacaag caagatggta       60

agcgctattg ttttatatgt gcttttggcg gcggcggcgc attctgcctt tgcgggatcc      120

caggttcagc tcgtagaatc tggagccgaa gtcaagaaac ctggagcatc agtaaaggtt      180

agctgcaagg cgtccggata caccttcacc aattacatca ttcattgggt gaagcaagag      240

ccgggccaag gtctggaatg gatcggatac tttaatccat acaaccacgg aacgaaatac      300

aacgaaaaat ttaaaggcag ggcaaccctc actgccgaca agtccattag taccgcgtac      360

atggagttgt ccagtttgag atcagaggat accgctgtct actactgcgc aaggagtggc      420

ccctacgctt ggttcgatac ctggggccag ggaactactg tcacggtatc cagtggtggc      480

ggtggtagcg acatcgtgct gacacagagc cctacaagcc tggccgtgtc tctgggacag      540

agagccacca tcagctgtag agccagcgag agcgtggaca acagcggcat cagcttcatg      600

aactggttcc agcagaagcc cggccagcct cctaagctgc tgatctacgc cgccagcaat      660

cctggaagcg gagtgcctgc cagattttct ggcagcggct ctggcaccga cttcagcctg      720

aacattcacc ccatggaaga ggacgacacc gccatgtact tttgccagca gagcaaagag      780

gtgccctgga cctttggcgg cggaaccgag ctggaaatca agagaagaag ggcagacgca      840

gctgccgctg gcggtggtgg aagcggtgga ggaggttctg gcggtggagg ttccggtgga      900

ggtggaacgg gtggaggcgg tagtgaagtg cagctggttc tgtctggcgg cggatttgtt      960

cagcctggcg gaagcctgaa gctgagctgt gctgccagcg gcttcacctt tagcagctac     1020

gccatgagct gggtccgaca gaaccctgag agaaggcttg tgtgggtcgc caccattaca     1080

ggcggcggac ggaacaccta ctatcccgat tctgtgaagg gcagattcac catcagccgg     1140

gacaacgcca agaacaccct gtacctgcag atgagcagcc tgagaagcga ggacaccgcc     1200

atgtactact gcaccagaca gggctacgac ggctacactt ggtttgccta ttggggccag     1260

ggcaccctgg tcacagtttc tggtggcgga ggatcagaca tcctgttaac acagagcccg     1320

gccacgttat cgctctctcc aggcgaaagg gccactttaa gctgcagagc ttcccaaaac     1380

ataggaactt cgatccaatg gtaccaacaa aagccgggtc aggcaccgag actgttgatc     1440

cgttccagct ctgaaagcat tagcggcatc tcctcgaggt tttcaggttc cggttcaggt     1500

accgacttca cgctgactat ctcttctctg gagcctgaag atttcgcagt atactactgt     1560

cagcagagca acacttggcc attcaccttc ggacagggta ctaagctgga gatcaaagcg     1620

gccgcgcatc atcaccacca tcaccac                                         1647


<210>  4
<211>  549
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  antiCD45-antiPD1 using scFv from 1B8 

<220>
<221>  misc_feature
<222>  (1)..(38)
<223>  Signal Peptide

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  Linker

<220>
<221>  misc_feature
<222>  (41)..(158)
<223>  heavy chain variable region of anti-CD45 scFv

<220>
<221>  misc_feature
<222>  (159)..(163)
<223>  Linker

<220>
<221>  misc_feature
<222>  (164)..(274)
<223>  light chain variable region of anti-PD1 scFv

<220>
<221>  misc_feature
<222>  (275)..(308)
<223>  Linker

<220>
<221>  misc_feature
<222>  (309)..(427)
<223>  heavy chain variable region of anti-PD1 scFv

<220>
<221>  misc_feature
<222>  (428)..(432)
<223>  Linker

<220>
<221>  misc_feature
<222>  (433)..(539)
<223>  light chain variable region of anti-CD45 scFv

<220>
<221>  misc_feature
<222>  (540)..(549)
<223>  His Tag

<400>  4

Met Leu Leu Val Asn Gln Ser His Gln Gly Phe Asn Lys Glu His Thr 
1               5                   10                  15      


Ser Lys Met Val Ser Ala Ile Val Leu Tyr Val Leu Leu Ala Ala Ala 
            20                  25                  30          


Ala His Ser Ala Phe Ala Gly Ser Gln Val Gln Leu Val Glu Ser Gly 
        35                  40                  45              


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
    50                  55                  60                  


Ser Gly Tyr Thr Phe Thr Asn Tyr Ile Ile His Trp Val Lys Gln Glu 
65                  70                  75                  80  


Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Phe Asn Pro Tyr Asn His 
                85                  90                  95      


Gly Thr Lys Tyr Asn Glu Lys Phe Lys Gly Arg Ala Thr Leu Thr Ala 
            100                 105                 110         


Asp Lys Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser 
        115                 120                 125             


Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly Pro Tyr Ala Trp 
    130                 135                 140                 


Phe Asp Thr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Thr Ser Leu Ala Val 
                165                 170                 175     


Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val 
            180                 185                 190         


Asp Asn Ser Gly Ile Ser Phe Met Asn Trp Phe Gln Gln Lys Pro Gly 
        195                 200                 205             


Gln Pro Pro Lys Leu Leu Ile Tyr Ala Ala Ser Asn Pro Gly Ser Gly 
    210                 215                 220                 


Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu 
225                 230                 235                 240 


Asn Ile His Pro Met Glu Glu Asp Asp Thr Ala Met Tyr Phe Cys Gln 
                245                 250                 255     


Gln Ser Lys Glu Val Pro Trp Thr Phe Gly Gly Gly Thr Glu Leu Glu 
            260                 265                 270         


Ile Lys Arg Arg Arg Ala Asp Ala Ala Ala Ala Gly Gly Gly Gly Ser 
        275                 280                 285             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Thr Gly 
    290                 295                 300                 


Gly Gly Gly Ser Glu Val Gln Leu Val Leu Ser Gly Gly Gly Phe Val 
305                 310                 315                 320 


Gln Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr 
                325                 330                 335     


Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Asn Pro Glu Arg Arg 
            340                 345                 350         


Leu Val Trp Val Ala Thr Ile Thr Gly Gly Gly Arg Asn Thr Tyr Tyr 
        355                 360                 365             


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
    370                 375                 380                 


Asn Thr Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala 
385                 390                 395                 400 


Met Tyr Tyr Cys Thr Arg Gln Gly Tyr Asp Gly Tyr Thr Trp Phe Ala 
                405                 410                 415     


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Gly Gly Gly Gly Ser 
            420                 425                 430         


Asp Ile Leu Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly 
        435                 440                 445             


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Asn Ile Gly Thr Ser 
    450                 455                 460                 


Ile Gln Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
465                 470                 475                 480 


Arg Ser Ser Ser Glu Ser Ile Ser Gly Ile Ser Ser Arg Phe Ser Gly 
                485                 490                 495     


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
            500                 505                 510         


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe 
        515                 520                 525             


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala His His 
    530                 535                 540                 


His His His His His 
545                 


<210>  5
<211>  1233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  antiCD45-IL2 fusion

<220>
<221>  misc_feature
<223>  encodes the peptide sequence at SEQ ID NO 6

<400>  5
atgaagacca tcatcgccct gagctacatc ttctgcctgg tgttcgccgg atcccaggtg       60

cagctggtgg aatctggcgc cgaagtgaaa aagcctggcg cctctgtgaa ggtgtcctgc      120

aaggccagcg gctacacctt taccaactac atcatccact gggtcaagca agagcccggc      180

cagggactcg agtggatcgg ctacttcaac ccctacaacc acggcaccaa gtacaacgag      240

aagttcaagg gcagagccac actgaccgcc gacaagagca tcagcaccgc ctacatggaa      300

ctgagcagcc tgagaagcga ggacaccgcc gtgtactact gtgccagatc cggaccttac      360

gcttggtttg acacatgggg ccagggcacc accgtgacag tttctagcgg cggcagcagc      420

agaagctcta gttctggcgg cggaggatct ggcggaggcg gagatattct gctgacacag      480

agccccgcca cactgtcact ttctccaggc gagagagcca ctctgagctg tagagccagc      540

cagaacatcg gcaccagcat ccagtggtat cagcagaaac ctggacaggc ccctcggctg      600

ctgatcagat ctagctccga gagcatctcc ggcatcagct ccagattttc tggcagcggc      660

tccggcaccg atttcaccct gaccataagc agcctggaac ctgaggattt cgccgtgtat      720

tattgccagc agagcaacac ctggcctttc acctttggcc aggggaccaa gctggaaatc      780

aaaggcggcg gaagctctgc ccctaccagc agcagcacca agaaaaccca actgcagctg      840

gaacacctcc tgctggacct gcagatgatc ctgaacggca tcaacaacta caagaacccc      900

aagctgaccc ggatgctgac cttcaagttc tacatgccca agaaggccac cgagctgaag      960

cacctccagt gcctggaaga ggaactgaag cccctggaag aagtgctgaa tctggcccag     1020

agcaagaact tccacctgag gcctagggac ctgatctcca acatcaacgt gatcgtgctg     1080

gaactgaaag gcagcgagac aaccttcatg tgcgagtacg ccgacgagac agctaccatc     1140

gtggaatttc tgaaccggtg gatcaccttc tgccagagca tcatctctac cctgacagcg     1200

gccgcacacc accatcatca ccatcaccac cat                                  1233


<210>  6
<211>  415
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  antiCD45-IL2 fusion

<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  Signal Peptide

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  Linker

<220>
<221>  misc_feature
<222>  (19)..(140)
<223>  heavy chain variable region of anti-CD45 scFv

<220>
<221>  misc_feature
<222>  (141)..(158)
<223>  Linker

<220>
<221>  misc_feature
<222>  (159)..(265)
<223>  Linker

<220>
<221>  misc_feature
<222>  (266)..(270)
<223>  light chain variable region of anti-CD45 scFv

<220>
<221>  misc_feature
<222>  (271)..(403)
<223>  IL-2

<220>
<221>  misc_feature
<222>  (404)..(415)
<223>  His Tag

<400>  6

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Phe Ala 
1               5                   10                  15      


Gly Ser Glu Val Gln Leu Gln Val Gln Leu Val Glu Ser Gly Ala Glu 
            20                  25                  30          


Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly 
        35                  40                  45              


Tyr Thr Phe Thr Asn Tyr Ile Ile His Trp Val Lys Gln Glu Pro Gly 
    50                  55                  60                  


Gln Gly Leu Glu Trp Ile Gly Tyr Phe Asn Pro Tyr Asn His Gly Thr 
65                  70                  75                  80  


Lys Tyr Asn Glu Lys Phe Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys 
                85                  90                  95      


Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly Pro Tyr Ala Trp Phe Asp 
        115                 120                 125             


Thr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Gly Ser Ser 
    130                 135                 140                 


Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Asp Ile 
145                 150                 155                 160 


Leu Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg 
                165                 170                 175     


Ala Thr Leu Ser Cys Arg Ala Ser Gln Asn Ile Gly Thr Ser Ile Gln 
            180                 185                 190         


Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Arg Ser 
        195                 200                 205             


Ser Ser Glu Ser Ile Ser Gly Ile Ser Ser Arg Phe Ser Gly Ser Gly 
    210                 215                 220                 


Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp 
225                 230                 235                 240 


Phe Ala Val Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe Thr Phe 
                245                 250                 255     


Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly Gly Gly Ser Ser Ala Pro 
            260                 265                 270         


Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu Leu 
        275                 280                 285             


Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn Pro 
    290                 295                 300                 


Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala 
305                 310                 315                 320 


Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu 
                325                 330                 335     


Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro 
            340                 345                 350         


Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly 
        355                 360                 365             


Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile 
    370                 375                 380                 


Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser 
385                 390                 395                 400 


Thr Leu Thr Ala Ala Ala His His His His His His His His His 
                405                 410                 415 


<210>  7
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  misc_feature
<223>  Linker peptide

<400>  7

Arg Arg Ala Asp Ala Ala Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Thr Gly Gly Gly Gly 
            20                  25                  30          


Ser 
    


<210>  8
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  lentiviral delivery system targeting human PD-1 sequence

<400>  8
caccgcgact ggccagggcg cctgt                                             25


<210>  9
<211>  1497
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-PD1 Nivo v0.1

<400>  9
caggttcagc tcgtagaatc tggagccgaa gtcaagaaac ctggagcatc agtaaaggtt       60

agctgcaagg cgtccggata caccttcacc aattacatca ttcattgggt gaagcaagag      120

ccgggccaag gtctggaatg gatcggatac tttaatccat acaaccacgg aacgaaatac      180

aacgaaaaat ttaaaggcag ggcaaccctc actgccgaca agtccattag taccgcgtac      240

atggagttgt ccagtttgag atcagaggat accgctgtct actactgcgc aaggagtggc      300

ccctacgctt ggttcgatac ctggggccag ggaactactg tcacggtatc cagtggtggc      360

ggtggtagcg aaatcgtact gacccagagc cctgcgactt tatctttatc tcctggcgaa      420

agggctaccc tgtcatgtcg cgcatctcag tccgtctcat cgtacctcgc ctggtaccag      480

cagaaacccg gacaagcgcc tcgtcttttg atctatgacg cctctaaccg tgccaccggt      540

attccagctc gtttctctgg ctccggttcc ggcacagact tcactttgac gatttcttca      600

ctcgaacctg aagactttgc agtctattac tgccagcaga gttccaactg gcctcgcacc      660

ttcggccaga ctaaggtgga gattaaaaga agggcagacg ccgccgccgc tggcggtggt      720

ggatctgggg gaggaggttc tggcggggga ggttccggtg gaggtgggac ggggggaggc      780

ggtagtcaag ttcagttggt ggaatctggc ggcggagtcg tacagccggg acgctcattg      840

agactggact gtaaggctag tggaatcaca ttcagcaact ccggaatgca ctgggttagg      900

caagctcccg gcaaaggatt ggaatgggtc gccgtaattt ggtacgacgg atctaagagg      960

tactacgctg acagcgttaa gggtaggttc acaatctcca gggataactc aaagaacaca     1020

ctttttctgc aaatgaactc cctccgtgcc gaggacaccg ccgtttacta ctgtgcaact     1080

aacgatgatt attggggtca gggtactctg gtcactgtca gctccggtgg cggaggatca     1140

gacatcctgt taacacagag cccggccacg ttatcgctct ctccaggcga aagggccact     1200

ttaagctgca gagcttccca aaacatagga acttcgatcc aatggtacca acaaaagccg     1260

ggtcaggcac cgagactgtt gatccgttcc agctctgaaa gcattagcgg catctcctcg     1320

aggttttcag gttccggttc aggtaccgac ttcacgctga ctatctcttc tctggagcct     1380

gaagatttcg cagtatacta ctgtcagcag agcaacactt ggccattcac cttcggacag     1440

ggtactaagc tggagatcaa agcggccgcg catcatcacc accatcacca ccattag        1497


<210>  10
<211>  498
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-PD1 Nivo v0.1

<400>  10

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Ile Ile His Trp Val Lys Gln Glu Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Phe Asn Pro Tyr Asn His Gly Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Pro Tyr Ala Trp Phe Asp Thr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr 
        115                 120                 125             


Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu 
    130                 135                 140                 


Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Ala Trp Tyr Gln 
145                 150                 155                 160 


Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Asp Ala Ser Asn 
                165                 170                 175     


Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            180                 185                 190         


Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Val 
        195                 200                 205             


Tyr Tyr Cys Gln Gln Ser Ser Asn Trp Pro Arg Thr Phe Gly Gln Thr 
    210                 215                 220                 


Lys Val Glu Ile Lys Arg Arg Ala Asp Ala Ala Ala Ala Gly Gly Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
                245                 250                 255     


Thr Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly 
            260                 265                 270         


Val Val Gln Pro Gly Arg Ser Leu Arg Leu Asp Cys Lys Ala Ser Gly 
        275                 280                 285             


Ile Thr Phe Ser Asn Ser Gly Met His Trp Val Arg Gln Ala Pro Gly 
    290                 295                 300                 


Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Lys Arg 
305                 310                 315                 320 


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                325                 330                 335     


Ser Lys Asn Thr Leu Phe Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            340                 345                 350         


Thr Ala Val Tyr Tyr Cys Ala Thr Asn Asp Asp Tyr Trp Gly Gln Gly 
        355                 360                 365             


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp Ile Leu Leu 
    370                 375                 380                 


Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr 
385                 390                 395                 400 


Leu Ser Cys Arg Ala Ser Gln Asn Ile Gly Thr Ser Ile Gln Trp Tyr 
                405                 410                 415     


Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Arg Ser Ser Ser 
            420                 425                 430         


Glu Ser Ile Ser Gly Ile Ser Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        435                 440                 445             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala 
    450                 455                 460                 


Val Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe Thr Phe Gly Gln 
465                 470                 475                 480 


Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala His His His His His His 
                485                 490                 495     


His His 
        


<210>  11
<211>  1497
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-PD1 Nivo Stabilized

<400>  11
caggttcagc tcgtagaatc tggagccgaa gtcaagaaac ctggagcatc agtaaaggtt       60

agctgcaagg cgtccggata caccttcacc aattacatca ttcattgggt gaagcaagag      120

ccgggccaag gtctggaatg gatcggatac tttaatccat acaaccacgg aacgaaatac      180

aacgaaaaat ttaaaggcag ggcaaccctc actgccgaca agtccattag taccgcgtac      240

atggagttgt ccagtttgac ctcagaggat accgctgtct actactgcgc aaggagtggc      300

ccctacgctt ggttcgatac ctggggccag ggaactactg tcacggtatc cagtggtggc      360

ggtggtagcg aaatcgtact gacccagagc cctgcgactt tatctttatc tcctggcgaa      420

agggctaccc tgtcatgtcg cgcatctcag tccgtctcat cgtacctcgc ctggtaccag      480

cagaaacccg gacaagcgcc tcgtcttttg atctatgacg cctctaaccg tgccaccggt      540

attccagctc gtttctctgg ctccggttcc ggcacagact tcactttgac gatttcttca      600

ctcgaacctg aagactttgc agtctattac tgccagcaga gttccaactg gcctcgcacc      660

ttcggccaga ctaaggtgga gattaaaaga agggcagacg cggccgccgc tggcggtggt      720

ggaagcggtg gaggaggatc tggcggtgga ggttccggtg gaggaggaac gggtggtgga      780

ggtagtcaag ttcagttggt ggaatctggc ggcggagtcg tacagccggg acgctcattg      840

agactggact gtaaggctag tggaatcaca ttcagcaact ccggaatgca ctgggttagg      900

caagctcccg gcaaaggatt ggaatgggtc gccgtaattt ggtacgacgg atctaagagg      960

tactacgctg acagcgttaa gggtaggttc acaatctcca gggataactc aaagaacaca     1020

ctttttctgc aaatgaactc cctcaccgcc gaggacaccg ccgtttacta ctgtgcaact     1080

aacgatgatt attggggtca gggtactctg gtcactgtca gctccggtgg cggaggatca     1140

gacatcctgt taacacagag cccggccacg ttatcgctct ctccaggcga aagggccact     1200

ttaagctgca gagcttccca aaacatagga acttcgatcc aatggtacca acaaaagccg     1260

ggtcaggcac cgagactgtt gatccgttcc agctctgaaa gcattagcgg catctcctcg     1320

aggttttcag gttccggttc aggtaccgac ttcacgctga ctatctcttc tctggagcct     1380

gaagatttcg cagtatacta ctgtcagcag agcaacactt ggccattcac cttcggacag     1440

ggtactaagc tggagatcaa agcggccgcg catcatcacc accatcacca ccattaa        1497


<210>  12
<211>  498
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-PD1 Nivo Stabilized

<400>  12

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Ile Ile His Trp Val Lys Gln Glu Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Phe Asn Pro Tyr Asn His Gly Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Pro Tyr Ala Trp Phe Asp Thr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr 
        115                 120                 125             


Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu 
    130                 135                 140                 


Ser Cys Arg Ala Ser Gln Ser Val Ser Ser Tyr Leu Ala Trp Tyr Gln 
145                 150                 155                 160 


Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr Asp Ala Ser Asn 
                165                 170                 175     


Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            180                 185                 190         


Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Val 
        195                 200                 205             


Tyr Tyr Cys Gln Gln Ser Ser Asn Trp Pro Arg Thr Phe Gly Gln Thr 
    210                 215                 220                 


Lys Val Glu Ile Lys Arg Arg Ala Asp Ala Ala Ala Ala Gly Gly Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
                245                 250                 255     


Thr Gly Gly Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly 
            260                 265                 270         


Val Val Gln Pro Gly Arg Ser Leu Arg Leu Asp Cys Lys Ala Ser Gly 
        275                 280                 285             


Ile Thr Phe Ser Asn Ser Gly Met His Trp Val Arg Gln Ala Pro Gly 
    290                 295                 300                 


Lys Gly Leu Glu Trp Val Ala Val Ile Trp Tyr Asp Gly Ser Lys Arg 
305                 310                 315                 320 


Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn 
                325                 330                 335     


Ser Lys Asn Thr Leu Phe Leu Gln Met Asn Ser Leu Thr Ala Glu Asp 
            340                 345                 350         


Thr Ala Val Tyr Tyr Cys Ala Thr Asn Asp Asp Tyr Trp Gly Gln Gly 
        355                 360                 365             


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp Ile Leu Leu 
    370                 375                 380                 


Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr 
385                 390                 395                 400 


Leu Ser Cys Arg Ala Ser Gln Asn Ile Gly Thr Ser Ile Gln Trp Tyr 
                405                 410                 415     


Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Arg Ser Ser Ser 
            420                 425                 430         


Glu Ser Ile Ser Gly Ile Ser Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        435                 440                 445             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala 
    450                 455                 460                 


Val Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe Thr Phe Gly Gln 
465                 470                 475                 480 


Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala His His His His His His 
                485                 490                 495     


His His 
        


<210>  13
<211>  1533
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-PD1 Pembro

<400>  13
caggttcagc tcgtagaatc tggagccgaa gtcaagaaac ctggagcatc agtaaaggtt       60

agctgcaagg cgtccggata caccttcacc aattacatca ttcattgggt gaagcaagag      120

ccgggccaag gtctggaatg gatcggatac tttaatccat acaaccacgg aacgaaatac      180

aacgaaaaat ttaaaggcag ggcaaccctc actgccgaca agtccattag taccgcgtac      240

atggagttgt ccagtttgag atcagaggat accgctgtct actactgcgc aaggagtggc      300

ccctacgctt ggttcgatac ctggggccag ggaactactg tcacggtatc cagtggtggc      360

ggtggtagcg agatcgtgct gacacagtct cccgccacac tgtcactgtc tccaggcgaa      420

agagccacac tgagctgcag agcctctaag ggcgtgtcca caagcggcta cagctacctg      480

cactggtatc agcagaagcc cggacaggct cccagactgc tgatctacct ggcctcctac      540

ctggaaagcg gagtgcctgc cagattttct ggcagcggct ctggcaccga tttcaccctg      600

accataagca gcctggaacc tgaggacttc gccgtgtact actgccagca cagcagagat      660

ctgcccctga catttggcgg aggcaccaag gtggaaatca agagaagggc agacgcggcc      720

gccgctggag gtggaggtag tggcggaggt ggatctggtg gaggaggctc aggtggaggc      780

ggtacgggtg gcggaggtag tcaagttcaa ctggtgcaga gcggcgtgga agtgaagaaa      840

cctggcgcct ctgtgaaggt gtcctgcaag gccagcggct ataccttcac caactactac      900

atgtactggg tccgacaggc ccctggacag ggacttgaat ggatgggcgg catcaaccct      960

agcaacggcg gcaccaactt caacgagaag ttcaagaaca gagtgaccct gacaaccgac     1020

agcagcacca ccacagccta catggaactg aagtccctgc agttcgacga caccgccgtg     1080

tattattgcg ccagacggga ctacagattc gacatgggct ttgactattg gggccagggc     1140

actaccgtaa cagtgtcttc tggtggcgga ggatcagaca tcctgttaac acagagcccg     1200

gccacgttat cgctctctcc aggcgaaagg gccactttaa gctgcagagc ttcccaaaac     1260

ataggaactt cgatccaatg gtaccaacaa aagccgggtc aggcaccgag actgttgatc     1320

cgttccagct ctgaaagcat tagcggcatc tcctcgaggt tttcaggttc cggttcaggt     1380

accgacttca cgctgactat ctcttctctg gagcctgaag atttcgcagt atactactgt     1440

cagcagagca acacttggcc attcaccttc ggacagggta ctaagctgga gatcaaagcg     1500

gccgcgcatc accaccacca tcaccaccat taa                                  1533


<210>  14
<211>  510
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-PD1 Pembro

<400>  14

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Ile Ile His Trp Val Lys Gln Glu Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Phe Asn Pro Tyr Asn His Gly Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Pro Tyr Ala Trp Phe Asp Thr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Ile Val Leu Thr 
        115                 120                 125             


Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu 
    130                 135                 140                 


Ser Cys Arg Ala Ser Lys Gly Val Ser Thr Ser Gly Tyr Ser Tyr Leu 
145                 150                 155                 160 


His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Tyr 
                165                 170                 175     


Leu Ala Ser Tyr Leu Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
            180                 185                 190         


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu 
        195                 200                 205             


Asp Phe Ala Val Tyr Tyr Cys Gln His Ser Arg Asp Leu Pro Leu Thr 
    210                 215                 220                 


Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Arg Ala Asp Ala Ala 
225                 230                 235                 240 


Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
                245                 250                 255     


Ser Gly Gly Gly Gly Thr Gly Gly Gly Gly Ser Gln Val Gln Leu Val 
            260                 265                 270         


Gln Ser Gly Val Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser 
        275                 280                 285             


Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr Tyr Met Tyr Trp Val 
    290                 295                 300                 


Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Gly Ile Asn Pro 
305                 310                 315                 320 


Ser Asn Gly Gly Thr Asn Phe Asn Glu Lys Phe Lys Asn Arg Val Thr 
                325                 330                 335     


Leu Thr Thr Asp Ser Ser Thr Thr Thr Ala Tyr Met Glu Leu Lys Ser 
            340                 345                 350         


Leu Gln Phe Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg Asp Tyr 
        355                 360                 365             


Arg Phe Asp Met Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr 
    370                 375                 380                 


Val Ser Ser Gly Gly Gly Gly Ser Asp Ile Leu Leu Thr Gln Ser Pro 
385                 390                 395                 400 


Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg 
                405                 410                 415     


Ala Ser Gln Asn Ile Gly Thr Ser Ile Gln Trp Tyr Gln Gln Lys Pro 
            420                 425                 430         


Gly Gln Ala Pro Arg Leu Leu Ile Arg Ser Ser Ser Glu Ser Ile Ser 
        435                 440                 445             


Gly Ile Ser Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
    450                 455                 460                 


Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys 
465                 470                 475                 480 


Gln Gln Ser Asn Thr Trp Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu 
                485                 490                 495     


Glu Ile Lys Ala Ala Ala His His His His His His His His 
            500                 505                 510 


<210>  15
<211>  1518
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-PD1 Cl19

<400>  15
caggttcagc tcgtagaatc tggagccgaa gtcaagaaac ctggagcatc agtaaaggtt       60

agctgcaagg cgtccggata caccttcacc aattacatca ttcattgggt gaagcaagag      120

ccgggccaag gtctggaatg gatcggatac tttaatccat acaaccacgg aacgaaatac      180

aacgaaaaat ttaaaggcag ggcaaccctc actgccgaca agtccattag taccgcgtac      240

atggagttgt ccagtttgac ctcagaggat accgctgtct actactgcgc aaggagtggc      300

ccctacgctt ggttcgatac ctggggccag ggaactactg tcacggtatc cagtggtggc      360

ggtggtagcg aaaatgtgct cacccagtct ccagcaatca tgtctgcatc tccaggggaa      420

aaggtcacca tgacctgcag ggccagctca agtgtaattt ccagttactt gcactggtac      480

cagcagaagt caggtgcctc ccccaaactc tggatttata gcacttccaa cttggcttct      540

ggagtccctg atcgattcag tggcagtggg tctgggacct cttactctct cacaatcagc      600

agtgtggagg ctgaagatgc tgccacttat tactgccagc agtacaatgg ttacccgctc      660

acgttcggtg ctgggaccaa gctggaaata aaaagaaggg cagacgcggc cgccgctggc      720

ggtggtggaa gcggtggagg aggatctggc ggtggaggtt ccggtggagg aggaacgggt      780

ggtggaggta gtcaggttca gctacagcag tctggggctg agctggtgaa gcctggggcc      840

tcagtgaaga tgtcctgcaa ggcttttggc tacaccttca ctacctatcc aatagagtgg      900

atgaagcaga atcatgggaa gagcctagag tggattggaa attttcatcc ttacaatgat      960

gatactaagt acaatgaaaa attcaagggc aaggccaaat tgactgtaga aaaatcctct     1020

accacagtct acttggagct cagccgatta acatctgacg actctgctgt ttattactgt     1080

gcaagggaga actacggtag tcacgggggt tttgtttact ggggccaagg gactctggtc     1140

accgtcggtg gcggaggatc agacatcctg ttaacacaga gcccggccac gttatcgctc     1200

tctccaggcg aaagggccac tttaagctgc agagcttccc aaaacatagg aacttcgatc     1260

caatggtacc aacaaaagcc gggtcaggca ccgagactgt tgatccgttc cagctctgaa     1320

agcattagcg gcatctcctc gaggttttca ggttccggtt caggtaccga cttcacgctg     1380

actatctctt ctctggagcc tgaagatttc gcagtatact actgtcagca gagcaacact     1440

tggccattca ccttcggaca gggtactaag ctggagatca aagcggccgc gcatcatcac     1500

caccatcacc accattaa                                                   1518


<210>  16
<211>  505
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-PD1 Cl19

<400>  16

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Ile Ile His Trp Val Lys Gln Glu Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Phe Asn Pro Tyr Asn His Gly Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Pro Tyr Ala Trp Phe Asp Thr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Glu Asn Val Leu Thr 
        115                 120                 125             


Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met 
    130                 135                 140                 


Thr Cys Arg Ala Ser Ser Ser Val Ile Ser Ser Tyr Leu His Trp Tyr 
145                 150                 155                 160 


Gln Gln Lys Ser Gly Ala Ser Pro Lys Leu Trp Ile Tyr Ser Thr Ser 
                165                 170                 175     


Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly 
            180                 185                 190         


Thr Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu Asp Ala Ala 
        195                 200                 205             


Thr Tyr Tyr Cys Gln Gln Tyr Asn Gly Tyr Pro Leu Thr Phe Gly Ala 
    210                 215                 220                 


Gly Thr Lys Leu Glu Ile Lys Arg Arg Ala Asp Ala Ala Ala Ala Gly 
225                 230                 235                 240 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
                245                 250                 255     


Gly Gly Thr Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Gln Ser Gly 
            260                 265                 270         


Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala 
        275                 280                 285             


Phe Gly Tyr Thr Phe Thr Thr Tyr Pro Ile Glu Trp Met Lys Gln Asn 
    290                 295                 300                 


His Gly Lys Ser Leu Glu Trp Ile Gly Asn Phe His Pro Tyr Asn Asp 
305                 310                 315                 320 


Asp Thr Lys Tyr Asn Glu Lys Phe Lys Gly Lys Ala Lys Leu Thr Val 
                325                 330                 335     


Glu Lys Ser Ser Thr Thr Val Tyr Leu Glu Leu Ser Arg Leu Thr Ser 
            340                 345                 350         


Asp Asp Ser Ala Val Tyr Tyr Cys Ala Arg Glu Asn Tyr Gly Ser His 
        355                 360                 365             


Gly Gly Phe Val Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Gly Gly 
    370                 375                 380                 


Gly Gly Ser Asp Ile Leu Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu 
385                 390                 395                 400 


Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Asn Ile 
                405                 410                 415     


Gly Thr Ser Ile Gln Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg 
            420                 425                 430         


Leu Leu Ile Arg Ser Ser Ser Glu Ser Ile Ser Gly Ile Ser Ser Arg 
        435                 440                 445             


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
    450                 455                 460                 


Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Ser Asn Thr 
465                 470                 475                 480 


Trp Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Ala Ala 
                485                 490                 495     


Ala His His His His His His His His 
            500                 505 


<210>  17
<211>  720
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-Db-4

<400>  17
caggtgcagc tggtggagtc aggagccgaa gtgaaaaagc ctggggcttc agtgaaggtg       60

tcctgcaagg cctctggata cacattcact aattatatta tccactgggt gaagcaggag      120

cctggtcagg gccttgaatg gattggatat tttaatcctt acaatcatgg tactaagtac      180

aatgagaagt tcaaaggcag ggccacacta actgcagaca aatccatcag cacagcctac      240

atggagctca gcagcctgcg ctctgaggac actgcggtct actactgtgc aagatcagga      300

ccctatgcct ggtttgacac ctggggccaa gggaccacgg tcaccgtctc ctcaggaggt      360

ggcggtagtg acattctgct gacccagtct ccagccaccc tgtctctgag tccaggagaa      420

agagccactc tctcctgcag ggccagtcag aacattggca caagcataca gtggtatcaa      480

caaaaaccag gtcaggctcc aaggcttctc ataaggtctt cttctgagtc tatctctggg      540

atctcttcca ggtttagtgg cagtggatca gggacagatt ttactcttac catcagcagt      600

ctggagcctg aagattttgc agtgtattac tgtcaacaaa gtaatacctg gccattcacg      660

ttcggccagg ggaccaagct ggagatcaaa gcggccgcgc atcatcacca ccatcaccac      720


<210>  18
<211>  240
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-Db-4

<400>  18

Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Ile Ile His Trp Val Lys Gln Glu Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Phe Asn Pro Tyr Asn His Gly Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Pro Tyr Ala Trp Phe Asp Thr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Asp Ile Leu Leu Thr 
        115                 120                 125             


Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu 
    130                 135                 140                 


Ser Cys Arg Ala Ser Gln Asn Ile Gly Thr Ser Ile Gln Trp Tyr Gln 
145                 150                 155                 160 


Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Arg Ser Ser Ser Glu 
                165                 170                 175     


Ser Ile Ser Gly Ile Ser Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
            180                 185                 190         


Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Val 
        195                 200                 205             


Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe Thr Phe Gly Gln Gly 
    210                 215                 220                 


Thr Lys Leu Glu Ile Lys Ala Ala Ala His His His His His His His 
225                 230                 235                 240 


<210>  19
<211>  1170
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-PD1 VHH

<400>  19
gatgtgcagc tggtggaatc tggtggcgga gttgttcagc ctggcggctc tctgagactg       60

tcttgtgccg cctctggctc tatcgcctct atccatgcca tgggctggtt cagacaggcc      120

cctggcaaag aaagggaatt cgtggccgtg atcacttgga gcggcggcat cacctactac      180

gccgattctg tgaagggcag attcaccatc agccgggaca acagcaagaa caccgtgtac      240

ctgcagatga acagcctgcg gcctgaggac acagccctgt attattgtgc cggcgacaag      300

caccagtcct cttggtacga ttattggggc cagggcaccc tggtcaccgt ttcttctgga      360

ggtggttctt tggaagttct gtttcaaggg cctggatctg gatctgacat cctgttaaca      420

cagagcccgg ccacgttatc gctctctcca ggcgaaaggg ccactttaag ctgcagagct      480

tcccaaaaca taggaacttc gatccaatgg taccaacaaa agccgggtca ggcaccgaga      540

ctgttgatcc gttccagctc tgaaagcatt agcggcatct cctcgaggtt ttcaggttcc      600

ggttcaggta ccgacttcac gctgactatc tcttctctgg agcctgaaga tttcgcagta      660

tactactgtc agcagagcaa cacttggcca ttcaccttcg gacagggtac taagctggag      720

atcaaaggcg gcagcaccag aagctctagt tctggtggag gaggatctgg cggaggcgga      780

caggttcagc tcgtagaatc tggagccgaa gtcaagaaac ctggagcatc agtaaaggtt      840

agctgcaagg cgtccggata caccttcacc aattacatca ttcattgggt gaagcaagag      900

ccgggccaag gtctggaatg gatcggatac tttaatccat acaaccacgg aacgaaatac      960

aacgaaaaat ttaaaggcag ggcaaccctc actgccgaca agtccattag taccgcgtac     1020

atggagttgt ccagtttgag atcagaggat accgctgtct actactgcgc aaggagtggc     1080

ccctacgctt ggttcgatac ctggggccag ggaactactg tcacggtatc cagtgcggcc     1140

gcgcatcatc accaccatca ccaccattaa                                      1170


<210>  20
<211>  389
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-PD1 VHH

<400>  20

Asp Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Ala Ser Ile His 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Val Ile Thr Trp Ser Gly Gly Ile Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Gly Asp Lys His Gln Ser Ser Trp Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Gly Gly Gly Ser Leu Glu Val Leu Phe 
        115                 120                 125             


Gln Gly Pro Gly Ser Gly Ser Asp Ile Leu Leu Thr Gln Ser Pro Ala 
    130                 135                 140                 


Thr Leu Ser Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Arg Ala 
145                 150                 155                 160 


Ser Gln Asn Ile Gly Thr Ser Ile Gln Trp Tyr Gln Gln Lys Pro Gly 
                165                 170                 175     


Gln Ala Pro Arg Leu Leu Ile Arg Ser Ser Ser Glu Ser Ile Ser Gly 
            180                 185                 190         


Ile Ser Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu 
        195                 200                 205             


Thr Ile Ser Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Ser Asn Thr Trp Pro Phe Thr Phe Gly Gln Gly Thr Lys Leu Glu 
225                 230                 235                 240 


Ile Lys Gly Gly Ser Thr Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser 
                245                 250                 255     


Gly Gly Gly Gly Gln Val Gln Leu Val Glu Ser Gly Ala Glu Val Lys 
            260                 265                 270         


Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr 
        275                 280                 285             


Phe Thr Asn Tyr Ile Ile His Trp Val Lys Gln Glu Pro Gly Gln Gly 
    290                 295                 300                 


Leu Glu Trp Ile Gly Tyr Phe Asn Pro Tyr Asn His Gly Thr Lys Tyr 
305                 310                 315                 320 


Asn Glu Lys Phe Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Ile 
                325                 330                 335     


Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala 
            340                 345                 350         


Val Tyr Tyr Cys Ala Arg Ser Gly Pro Tyr Ala Trp Phe Asp Thr Trp 
        355                 360                 365             


Gly Gln Gly Thr Thr Val Thr Val Ser Ser Ala Ala Ala His His His 
    370                 375                 380                 


His His His His His 
385                 


<210>  21
<211>  1194
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-PD1 VHH Extended

<400>  21
gatgtgcagc tggtggaatc tggtggcgga gttgttcagc ctggcggctc tctgagactg       60

tcttgtgccg cctctggctc tatcgcctct atccatgcca tgggctggtt cagacaggcc      120

cctggcaaag aaagggaatt cgtggccgtg atcacttgga gcggcggcat cacctactac      180

gccgattctg tgaagggcag attcaccatc agccgggaca acagcaagaa caccgtgtac      240

ctgcagatga acagcctgcg gcctgaggac acagccctgt attattgtgc cggcgacaag      300

caccagtcct cttggtacga ttattggggc cagggcaccc tggtcaccgt ttcttctggt      360

tctggttctg gaggtggttc tttggaagtt ctgtttcaag ggcctggagg ttcttcagga      420

tcaggatctg acatcctgtt aacacagagc ccggccacgt tatcgctctc tccaggcgaa      480

agggccactt taagctgcag agcttcccaa aacataggaa cttcgatcca atggtaccaa      540

caaaagccgg gtcaggcacc gagactgttg atccgttcca gctctgaaag cattagcggc      600

atctcctcga ggttttcagg ttccggttca ggtaccgact tcacgctgac tatctcttct      660

ctggagcctg aagatttcgc agtatactac tgtcagcaga gcaacacttg gccattcacc      720

ttcggacagg gtactaagct ggagatcaaa ggcggcagca ccagaagctc tagttctggt      780

ggaggaggat ctggcggagg cggacaggtt cagctcgtag aatctggagc cgaagtcaag      840

aaacctggag catcagtaaa ggttagctgc aaggcgtccg gatacacctt caccaattac      900

atcattcatt gggtgaagca agagccgggc caaggtctgg aatggatcgg atactttaat      960

ccatacaacc acggaacgaa atacaacgaa aaatttaaag gcagggcaac cctcactgcc     1020

gacaagtcca ttagtaccgc gtacatggag ttgtccagtt tgagatcaga ggataccgct     1080

gtctactact gcgcaaggag tggcccctac gcttggttcg atacctgggg ccagggaact     1140

actgtcacgg tatccagtgc ggccgcgcat catcaccacc atcaccacca ttaa           1194


<210>  22
<211>  397
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-PD1 VHH Extended

<400>  22

Asp Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Ala Ser Ile His 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Val Ile Thr Trp Ser Gly Gly Ile Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Leu Tyr Tyr Cys 
                85                  90                  95      


Ala Gly Asp Lys His Gln Ser Ser Trp Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Gly Ser Gly Ser Gly Gly Gly Ser Leu 
        115                 120                 125             


Glu Val Leu Phe Gln Gly Pro Gly Gly Ser Ser Gly Ser Gly Ser Asp 
    130                 135                 140                 


Ile Leu Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly Glu 
145                 150                 155                 160 


Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Asn Ile Gly Thr Ser Ile 
                165                 170                 175     


Gln Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile Arg 
            180                 185                 190         


Ser Ser Ser Glu Ser Ile Ser Gly Ile Ser Ser Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro Glu 
    210                 215                 220                 


Asp Phe Ala Val Tyr Tyr Cys Gln Gln Ser Asn Thr Trp Pro Phe Thr 
225                 230                 235                 240 


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly Gly Ser Thr Arg Ser 
                245                 250                 255     


Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu 
            260                 265                 270         


Val Glu Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val 
        275                 280                 285             


Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr Ile Ile His Trp 
    290                 295                 300                 


Val Lys Gln Glu Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Phe Asn 
305                 310                 315                 320 


Pro Tyr Asn His Gly Thr Lys Tyr Asn Glu Lys Phe Lys Gly Arg Ala 
                325                 330                 335     


Thr Leu Thr Ala Asp Lys Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser 
            340                 345                 350         


Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Ser Gly 
        355                 360                 365             


Pro Tyr Ala Trp Phe Asp Thr Trp Gly Gln Gly Thr Thr Val Thr Val 
    370                 375                 380                 


Ser Ser Ala Ala Ala His His His His His His His His 
385                 390                 395         


<210>  23
<211>  1143
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-PD1 mRIPR-PD1

<400>  23
caggtgcaac tggttgaaag cggcggagga ctggtgcacc ctggcgattc tctgagactg       60

tcttgtgccg ccagcggcag cgtgttcaat tctgccacaa tgggctggta cagacagagc      120

cctggcagcc agagagagct ggtcgctaca atcgttgtgg gcacccctac ctacgccgac      180

tctgtgaagg gcagattcac catcagccgg gacaacgcca agaacatcgt gtacctgcag      240

atgaacagcc tgaagcctga ggacaccgcc gtgtactact gcaactacag agccacctac      300

accagcggct acagcagaga ttattggggc cagggcaccc aagtgaccgt gtctagcgga      360

ggtggaggca caggcggatc tgacatcgtg atgacccagt ctccttccac cctgtctgca      420

tctgtaggag acagagtcac catcacttgc cgggccagtc agggtattag tagctggttg      480

gcctggtatc agcagaaacc agggagagcc cctaaggtct tgatctataa ggcatctact      540

ttagaaagtg gggtcccatc aaggttcagc ggcagtggat ctgggacaga tttcactctc      600

accatctcta gtctgcaacc tgaagatttt gcaacttact actgtcaaca gagttacagt      660

accccgtgga cgttcggcca ggggaccaag ctggaaatca agggcggcag caccagaagc      720

tctagttctg gtggaggagg atctggcgga ggcggagagg tgcagctggt tcagtctggg      780

ggaggcgtgg ttcagcctgg gaggtccctg agactctcct gtgcagcgtc tggattcacc      840

tttagtagct attggatgag ctgggtccgc caggctccag ggaaggggct ggagtgggtc      900

tcagctatta gtggtagtgg tggtagcaca tacgcagact ccgtgaaggg ccggttcacc      960

atctccagag acaattccaa gaacaccctg tatctgcaaa tgaactcgct aagagccgag     1020

gacacggccg tatattactg tgcgaaagag aactggggat cgtacttcga tctctgggga     1080

caaggaacca cggtaaccgt atcatcagcg gccgcgcatc atcaccacca tcaccaccat     1140

taa                                                                   1143


<210>  24
<211>  380
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-PD1 mRIPR-PD1

<400>  24

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val His Pro Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Val Phe Asn Ser Ala 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Ser Pro Gly Ser Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Val Val Gly Thr Pro Thr Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ile Val Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Tyr 
                85                  90                  95      


Arg Ala Thr Tyr Thr Ser Gly Tyr Ser Arg Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser Gly Gly Gly Gly Thr Gly Gly Ser Asp 
        115                 120                 125             


Ile Val Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly Asp 
    130                 135                 140                 


Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Trp Leu 
145                 150                 155                 160 


Ala Trp Tyr Gln Gln Lys Pro Gly Arg Ala Pro Lys Val Leu Ile Tyr 
                165                 170                 175     


Lys Ala Ser Thr Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
            180                 185                 190         


Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 
        195                 200                 205             


Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Trp Thr 
    210                 215                 220                 


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Gly Gly Ser Thr Arg Ser 
225                 230                 235                 240 


Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Glu Val Gln Leu 
                245                 250                 255     


Val Gln Ser Gly Gly Gly Val Val Gln Pro Gly Arg Ser Leu Arg Leu 
            260                 265                 270         


Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Trp Met Ser Trp 
        275                 280                 285             


Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Ala Ile Ser 
    290                 295                 300                 


Gly Ser Gly Gly Ser Thr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr 
305                 310                 315                 320 


Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu Gln Met Asn Ser 
                325                 330                 335     


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Lys Glu Asn Trp 
            340                 345                 350         


Gly Ser Tyr Phe Asp Leu Trp Gly Gln Gly Thr Thr Val Thr Val Ser 
        355                 360                 365             


Ser Ala Ala Ala His His His His His His His His 
    370                 375                 380 


<210>  25
<211>  813
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-CTLA4 mRIPR-CTLA4

<400>  25
caggtgcagc tggttgaatc tggcggagga ctggtgcacc ctggcgattc tctgagactg       60

tcttgtgccg ccagcggcag cgtgttcaat tctgccacaa tgggctggta cagacagagc      120

cctggcagcc agagagagct ggtcgctaca atcgttgtgg gcacccctac ctacgctgac      180

tctgtgaagg gcagattcac catcagccgg gacaacgcca agaacatcgt gtacctgcag      240

atgaacagcc tgaagcctga ggacaccgcc gtgtactact gcaactacag agccacctac      300

accagcggct acagcagaga ttattggggc cagggcaccc aagtgaccgt gtctagtgga      360

agcgagccca agattcccca gcctcagcct aaacctcagc cgcagccttt ggaagttctg      420

tttcaagggc ctggatctca ggtccagctt gtggaaagcg gaggtggact tgctcagcct      480

ggtggatcac tgagactgag ctgtgccgct tctggcagca caatcagctc tgtggccgtc      540

ggctggtata ggcagacacc tggaaaccag agagaatggg tcgccaccag cagcaccagc      600

tctacaacag ccacatacgc cgacagcgtg aaaggccggt ttaccatcag cagagacaat      660

gctaaaaaca ccatctatct ccagatgaac tctctcaagc ccgaggatac agccgtctat      720

tattgcaaga ccggcctgac caactgggga cagggaacac aagtcacagt gtccagcgcg      780

gccgcgcatc atcaccacca tcaccaccat taa                                   813


<210>  26
<211>  270
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-CTLA4 mRIPR-CTLA4

<400>  26

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val His Pro Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Val Phe Asn Ser Ala 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Ser Pro Gly Ser Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Val Val Gly Thr Pro Thr Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ile Val Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Tyr 
                85                  90                  95      


Arg Ala Thr Tyr Thr Ser Gly Tyr Ser Arg Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser Gly Ser Glu Pro Lys Ile Pro Gln Pro 
        115                 120                 125             


Gln Pro Lys Pro Gln Pro Gln Pro Leu Glu Val Leu Phe Gln Gly Pro 
    130                 135                 140                 


Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Gln Pro 
145                 150                 155                 160 


Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Thr Ile Ser 
                165                 170                 175     


Ser Val Ala Val Gly Trp Tyr Arg Gln Thr Pro Gly Asn Gln Arg Glu 
            180                 185                 190         


Trp Val Ala Thr Ser Ser Thr Ser Ser Thr Thr Ala Thr Tyr Ala Asp 
        195                 200                 205             


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr 
    210                 215                 220                 


Ile Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
225                 230                 235                 240 


Tyr Cys Lys Thr Gly Leu Thr Asn Trp Gly Gln Gly Thr Gln Val Thr 
                245                 250                 255     


Val Ser Ser Ala Ala Ala His His His His His His His His 
            260                 265                 270 


<210>  27
<211>  1545
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-PD1_CTLA4 dRIPR-PD1_CTLA4

<400>  27
caggtccagc ttgtggaaag cggaggtgga cttgctcagc ctggtggatc actgagactg       60

agctgtgccg cttctggcag cacaatcagc tctgtggccg tcggctggta taggcagaca      120

cctggaaacc agagagaatg ggtcgccacc agcagcacca gctctacaac agccacatac      180

gccgacagcg tgaaaggccg gtttaccatc agcagagaca atgctaaaaa caccatctat      240

ctccagatga actctctcaa gcccgaggat acagccgtct attattgcaa gaccggcctg      300

accaactggg gacagggaac acaagtcaca gtgtccagcg gtggagggcg cgccctggtg      360

ccgcgcggca gctctggaca ggtgcaactg gttgaaagcg gcggaggact ggtgcaccct      420

ggcgattctc tgagactgtc ttgtgccgcc agcggcagcg tgttcaattc tgccacaatg      480

ggctggtaca gacagagccc tggcagccag agagagctgg tcgctacaat cgttgtgggc      540

acccctacct acgccgactc tgtgaagggc agattcacca tcagccggga caacgccaag      600

aacatcgtgt acctgcagat gaacagcctg aagcctgagg acaccgccgt gtactactgc      660

aactacagag ccacctacac cagcggctac agcagagatt attggggcca gggcacccaa      720

gtgaccgtgt ctagcggagg tggttctttg gaagttctgt ttcaagggcc tggatctgga      780

tctgacatcg tgatgaccca gtctccttcc accctgtctg catctgtagg agacagagtc      840

accatcactt gccgggccag tcagggtatt agtagctggt tggcctggta tcagcagaaa      900

ccagggagag cccctaaggt cttgatctat aaggcatcta ctttagaaag tggggtccca      960

tcaaggttca gcggcagtgg atctgggaca gatttcactc tcaccatctc tagtctgcaa     1020

cctgaagatt ttgcaactta ctactgtcaa cagagttaca gtaccccgtg gacgttcggc     1080

caggggacca agctggaaat caagggcggc agcaccagaa gctctagttc tggtggagga     1140

ggatctggcg gaggcggaga ggtgcagctg gttcagtctg ggggaggcgt ggttcagcct     1200

gggaggtccc tgagactctc ctgtgcagcg tctggattca cctttagtag ctattggatg     1260

agctgggtcc gccaggctcc agggaagggg ctggagtggg tctcagctat tagtggtagt     1320

ggtggtagca catacgcaga ctccgtgaag ggccggttca ccatctccag agacaattcc     1380

aagaacaccc tgtatctgca aatgaactcg ctaagagccg aggacacggc cgtatattac     1440

tgtgcgaaag agaactgggg atcgtacttc gatctctggg gacaaggaac cacggtaacc     1500

gtatcatcag cggccgcgca tcaccaccac catcaccacc attaa                     1545


<210>  28
<211>  514
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-PD1_CTLA4 dRIPR-PD1_CTLA4

<400>  28

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Thr Ile Ser Ser Val 
            20                  25                  30          


Ala Val Gly Trp Tyr Arg Gln Thr Pro Gly Asn Gln Arg Glu Trp Val 
        35                  40                  45              


Ala Thr Ser Ser Thr Ser Ser Thr Thr Ala Thr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Ile Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Lys Thr Gly Leu Thr Asn Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
            100                 105                 110         


Ser Gly Gly Gly Arg Ala Leu Val Pro Arg Gly Ser Ser Gly Gln Val 
        115                 120                 125             


Gln Leu Val Glu Ser Gly Gly Gly Leu Val His Pro Gly Asp Ser Leu 
    130                 135                 140                 


Arg Leu Ser Cys Ala Ala Ser Gly Ser Val Phe Asn Ser Ala Thr Met 
145                 150                 155                 160 


Gly Trp Tyr Arg Gln Ser Pro Gly Ser Gln Arg Glu Leu Val Ala Thr 
                165                 170                 175     


Ile Val Val Gly Thr Pro Thr Tyr Ala Asp Ser Val Lys Gly Arg Phe 
            180                 185                 190         


Thr Ile Ser Arg Asp Asn Ala Lys Asn Ile Val Tyr Leu Gln Met Asn 
        195                 200                 205             


Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Tyr Arg Ala 
    210                 215                 220                 


Thr Tyr Thr Ser Gly Tyr Ser Arg Asp Tyr Trp Gly Gln Gly Thr Gln 
225                 230                 235                 240 


Val Thr Val Ser Ser Gly Gly Gly Ser Leu Glu Val Leu Phe Gln Gly 
                245                 250                 255     


Pro Gly Ser Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser Thr Leu 
            260                 265                 270         


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln 
        275                 280                 285             


Gly Ile Ser Ser Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Arg Ala 
    290                 295                 300                 


Pro Lys Val Leu Ile Tyr Lys Ala Ser Thr Leu Glu Ser Gly Val Pro 
305                 310                 315                 320 


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
                325                 330                 335     


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser 
            340                 345                 350         


Tyr Ser Thr Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
        355                 360                 365             


Gly Gly Ser Thr Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    370                 375                 380                 


Gly Gly Glu Val Gln Leu Val Gln Ser Gly Gly Gly Val Val Gln Pro 
385                 390                 395                 400 


Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
                405                 410                 415     


Ser Tyr Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
            420                 425                 430         


Trp Val Ser Ala Ile Ser Gly Ser Gly Gly Ser Thr Tyr Ala Asp Ser 
        435                 440                 445             


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu 
    450                 455                 460                 


Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr 
465                 470                 475                 480 


Cys Ala Lys Glu Asn Trp Gly Ser Tyr Phe Asp Leu Trp Gly Gln Gly 
                485                 490                 495     


Thr Thr Val Thr Val Ser Ser Ala Ala Ala His His His His His His 
            500                 505                 510         


His His 
        


<210>  29
<211>  486
<212>  DNA
<213>  Homo sapiens

<220>
<221>  misc_feature
<223>  Human PD-1

<400>  29
cacctgcact agcggccgcc ggatggtttc tggattctcc cgatagaccc tggaatcctc       60

ctacattcag ccccgctctg ctggtggtca cagagggcga taatgccacc ttcacctgta      120

gcttcagcaa caccagcgag agcttcgtgc tgaactggta cagaatgagc cccagcaacc      180

agaccgacaa gctggccgcc tttcctgagg atagaagcca gcctggacag gactgccggt      240

tcagagtgac acagctgccc aacggcagag acttccacat gtctgttgtg cgggccagaa      300

gaaacgacag cggcacatat ctgtgcggcg ccatttctct ggcccctaag gctcagatca      360

aagagagcct gagagccgag ctgagagtga ccgaaagacg ggctgaagtg cccacagctc      420

acccatctcc atctccaaga cctgccggcc agtttcagac cctcgttggc gcgccaagtg      480

caggtg                                                                 486


<210>  30
<211>  169
<212>  PRT
<213>  Homo sapiens

<220>
<221>  misc_feature
<223>  Human PD-1

<400>  30

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Phe Ala 
1               5                   10                  15      


Ala Ala Ala Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp Asn 
            20                  25                  30          


Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp Asn 
        35                  40                  45              


Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val Leu 
    50                  55                  60                  


Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala Ala 
65                  70                  75                  80  


Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg Val 
                85                  90                  95      


Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg Ala 
            100                 105                 110         


Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu Ala 
        115                 120                 125             


Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val Thr 
    130                 135                 140                 


Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg 
145                 150                 155                 160 


Pro Ala Gly Gln Phe Gln Thr Leu Val 
                165                 


<210>  31
<211>  543
<212>  DNA
<213>  Mus musculus

<220>
<221>  misc_feature
<223>  Mouse PD-1

<400>  31
ggatcctctg gatggctgct ggaagtgcct aacggccctt ggagaagcct gacattctac       60

cctgcctggc tgaccgtgtc tgaaggcgcc aatgccacct tcacctgtag cctgagcaat      120

tggagcgagg acctgatgct gaactggaac agactgagcc ccagcaacca gaccgaaaag      180

caggccgcct tctgcaacgg actgtctcag cctgtgcagg acgcccggtt ccagatcatc      240

cagctgccta acagacacga cttccacatg aacatcctgg acacccggcg gaacgacagc      300

ggcatctatc tgtgtggcgc catctctctg caccccaagg ccaagatcga ggaatctcct      360

ggcgccgagc tggtggtcac agagagaatc ctggaaacca gcacacgcta cccatctcca      420

tctccaaagc ctgagggcag attccagggc atgaaggcgg ccgcgggtgg cggtctgaac      480

gacatcttcg aggctcagaa aatcgaatgg cacgaacatc atcaccacca tcaccaccat      540

taa                                                                    543


<210>  32
<211>  180
<212>  PRT
<213>  Mus musculus

<220>
<221>  misc_feature
<223>  Mouse PD-1

<400>  32

Gly Ser Ser Gly Trp Leu Leu Glu Val Pro Asn Gly Pro Trp Arg Ser 
1               5                   10                  15      


Leu Thr Phe Tyr Pro Ala Trp Leu Thr Val Ser Glu Gly Ala Asn Ala 
            20                  25                  30          


Thr Phe Thr Cys Ser Leu Ser Asn Trp Ser Glu Asp Leu Met Leu Asn 
        35                  40                  45              


Trp Asn Arg Leu Ser Pro Ser Asn Gln Thr Glu Lys Gln Ala Ala Phe 
    50                  55                  60                  


Cys Asn Gly Leu Ser Gln Pro Val Gln Asp Ala Arg Phe Gln Ile Ile 
65                  70                  75                  80  


Gln Leu Pro Asn Arg His Asp Phe His Met Asn Ile Leu Asp Thr Arg 
                85                  90                  95      


Arg Asn Asp Ser Gly Ile Tyr Leu Cys Gly Ala Ile Ser Leu His Pro 
            100                 105                 110         


Lys Ala Lys Ile Glu Glu Ser Pro Gly Ala Glu Leu Val Val Thr Glu 
        115                 120                 125             


Arg Ile Leu Glu Thr Ser Thr Arg Tyr Pro Ser Pro Ser Pro Lys Pro 
    130                 135                 140                 


Glu Gly Arg Phe Gln Gly Met Lys Ala Ala Ala Gly Gly Gly Leu Asn 
145                 150                 155                 160 


Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu His His His His 
                165                 170                 175     


His His His His 
            180 


<210>  33
<211>  1752
<212>  DNA
<213>  Homo sapiens

<220>
<221>  misc_feature
<223>  Human CD45

<400>  33
ggatcccaga gccccacacc atctccaacc ggactgacca cagccaagat gcctagcgtg       60

cccctgtcta gcgatcccct gcctacacac accaccgctt ttagccctgc cagcaccttc      120

gagagagaga acgacttcag cgagacaacc acctctctga gccccgacaa caccagcaca      180

caggtgtccc cagacagcct ggataatgcc agcgccttca ataccaccgg cgtgtcctct      240

gtgcagaccc ctcatctgcc aacacacgcc gatagccaga caccttctgc cggcaccgat      300

acacagacct tttctggctc tgccgccaac gccaagctga accctacacc tggcagcaac      360

gccatctctg atgtgcctgg cgagagaagc accgccagca catttccaac agaccccgtg      420

tctcccctga ccaccacact gtctctggcc caccatagct ctgctgccct gcctgccaga      480

accagcaaca ccaccatcac cgccaatacc agcgacgcct acctgaacgc cagcgaaacc      540

acaacactga gccctagcgg aagcgccgtg atcagcacaa ccacaatcgc caccacacct      600

agcaagccta cctgcgacga gaagtacgcc aacatcaccg tggactacct gtacaacaaa      660

gagacaaagc tgttcacggc caagctcaac gtgaacgaga acgtggaatg cggcaacaat      720

acctgcacca acaacgaggt gcacaacctg accgagtgca agaacgcctc cgtgtccatc      780

agccacaaca gctgcacagc ccctgacaag accctgatcc tggatgtgcc tcctggcgtg      840

gaaaagttcc agctgcacga ttgcacccag gtggaaaagg ccgataccac catctgcctg      900

aagtggaaga acatcgagac attcacctgt gacacccaga acatcaccta ccggttccag      960

tgcggcaata tgatcttcga taacaaagaa atcaagctcg agaacctcga gcctgagcac     1020

gagtacaagt gcgacagcga gatcctgtat aacaatcaca agttcaccaa cgccagcaag     1080

atcatcaaga ccgacttcgg ctctcccggc gagccccaga tcatcttctg tagatctgag     1140

gccgctcacc agggcgtgat cacctggaat cctccacagc ggagcttcca caacttcacc     1200

ctgtgttaca tcaaagaaac cgagaaggac tgcctgaacc tggacaagaa cctcattaag     1260

tacgacctgc agaacctgaa gccgtacacc aaatacgtgc tgagcctgca cgcctacatt     1320

atcgccaagg tgcagagaaa tggcagcgcc gccatgtgcc acttcaccac aaaaagcgcc     1380

cctccaagcc aagtgtggaa catgaccgtg tctatgacca gcgacaactc catgcacgtg     1440

aagtgccggc ctccacggga tagaaatggc cctcacgaga gataccacct ggaagtcgag     1500

gccggaaata ccctcgtgcg gaacgagagc cacaagaact gcgacttcag agtgaaggac     1560

ctgcagtaca gcaccgacta caccttcaag gcctacttcc acaatggcga ctaccctggc     1620

gagcccttca tcctgcacca cagcaccagc tacaacagca aggcggccgc gggtggcggt     1680

ctgaacgaca tcttcgaggc tcagaaaatc gaatggcacg aacatcatca ccaccatcac     1740

caccattaat ga                                                         1752


<210>  34
<211>  582
<212>  PRT
<213>  Homo sapiens

<220>
<221>  misc_feature
<223>  Human CD45

<400>  34

Gly Ser Gln Ser Pro Thr Pro Ser Pro Thr Gly Leu Thr Thr Ala Lys 
1               5                   10                  15      


Met Pro Ser Val Pro Leu Ser Ser Asp Pro Leu Pro Thr His Thr Thr 
            20                  25                  30          


Ala Phe Ser Pro Ala Ser Thr Phe Glu Arg Glu Asn Asp Phe Ser Glu 
        35                  40                  45              


Thr Thr Thr Ser Leu Ser Pro Asp Asn Thr Ser Thr Gln Val Ser Pro 
    50                  55                  60                  


Asp Ser Leu Asp Asn Ala Ser Ala Phe Asn Thr Thr Gly Val Ser Ser 
65                  70                  75                  80  


Val Gln Thr Pro His Leu Pro Thr His Ala Asp Ser Gln Thr Pro Ser 
                85                  90                  95      


Ala Gly Thr Asp Thr Gln Thr Phe Ser Gly Ser Ala Ala Asn Ala Lys 
            100                 105                 110         


Leu Asn Pro Thr Pro Gly Ser Asn Ala Ile Ser Asp Val Pro Gly Glu 
        115                 120                 125             


Arg Ser Thr Ala Ser Thr Phe Pro Thr Asp Pro Val Ser Pro Leu Thr 
    130                 135                 140                 


Thr Thr Leu Ser Leu Ala His His Ser Ser Ala Ala Leu Pro Ala Arg 
145                 150                 155                 160 


Thr Ser Asn Thr Thr Ile Thr Ala Asn Thr Ser Asp Ala Tyr Leu Asn 
                165                 170                 175     


Ala Ser Glu Thr Thr Thr Leu Ser Pro Ser Gly Ser Ala Val Ile Ser 
            180                 185                 190         


Thr Thr Thr Ile Ala Thr Thr Pro Ser Lys Pro Thr Cys Asp Glu Lys 
        195                 200                 205             


Tyr Ala Asn Ile Thr Val Asp Tyr Leu Tyr Asn Lys Glu Thr Lys Leu 
    210                 215                 220                 


Phe Thr Ala Lys Leu Asn Val Asn Glu Asn Val Glu Cys Gly Asn Asn 
225                 230                 235                 240 


Thr Cys Thr Asn Asn Glu Val His Asn Leu Thr Glu Cys Lys Asn Ala 
                245                 250                 255     


Ser Val Ser Ile Ser His Asn Ser Cys Thr Ala Pro Asp Lys Thr Leu 
            260                 265                 270         


Ile Leu Asp Val Pro Pro Gly Val Glu Lys Phe Gln Leu His Asp Cys 
        275                 280                 285             


Thr Gln Val Glu Lys Ala Asp Thr Thr Ile Cys Leu Lys Trp Lys Asn 
    290                 295                 300                 


Ile Glu Thr Phe Thr Cys Asp Thr Gln Asn Ile Thr Tyr Arg Phe Gln 
305                 310                 315                 320 


Cys Gly Asn Met Ile Phe Asp Asn Lys Glu Ile Lys Leu Glu Asn Leu 
                325                 330                 335     


Glu Pro Glu His Glu Tyr Lys Cys Asp Ser Glu Ile Leu Tyr Asn Asn 
            340                 345                 350         


His Lys Phe Thr Asn Ala Ser Lys Ile Ile Lys Thr Asp Phe Gly Ser 
        355                 360                 365             


Pro Gly Glu Pro Gln Ile Ile Phe Cys Arg Ser Glu Ala Ala His Gln 
    370                 375                 380                 


Gly Val Ile Thr Trp Asn Pro Pro Gln Arg Ser Phe His Asn Phe Thr 
385                 390                 395                 400 


Leu Cys Tyr Ile Lys Glu Thr Glu Lys Asp Cys Leu Asn Leu Asp Lys 
                405                 410                 415     


Asn Leu Ile Lys Tyr Asp Leu Gln Asn Leu Lys Pro Tyr Thr Lys Tyr 
            420                 425                 430         


Val Leu Ser Leu His Ala Tyr Ile Ile Ala Lys Val Gln Arg Asn Gly 
        435                 440                 445             


Ser Ala Ala Met Cys His Phe Thr Thr Lys Ser Ala Pro Pro Ser Gln 
    450                 455                 460                 


Val Trp Asn Met Thr Val Ser Met Thr Ser Asp Asn Ser Met His Val 
465                 470                 475                 480 


Lys Cys Arg Pro Pro Arg Asp Arg Asn Gly Pro His Glu Arg Tyr His 
                485                 490                 495     


Leu Glu Val Glu Ala Gly Asn Thr Leu Val Arg Asn Glu Ser His Lys 
            500                 505                 510         


Asn Cys Asp Phe Arg Val Lys Asp Leu Gln Tyr Ser Thr Asp Tyr Thr 
        515                 520                 525             


Phe Lys Ala Tyr Phe His Asn Gly Asp Tyr Pro Gly Glu Pro Phe Ile 
    530                 535                 540                 


Leu His His Ser Thr Ser Tyr Asn Ser Lys Ala Ala Ala Gly Gly Gly 
545                 550                 555                 560 


Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu His His 
                565                 570                 575     


His His His His His His 
            580         


<210>  35
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 1 scFv-scFv

<400>  35
ggtggcggtg gtagc                                                        15


<210>  36
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 1 scFv-scFv

<400>  36

Gly Gly Gly Gly Ser 
1               5   


<210>  37
<211>  99
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 2 scFv-scFv

<400>  37
agaagggcag acgccgccgc cgctggcggt ggtggatctg ggggaggagg ttctggcggg       60

ggaggttccg gtggaggtgg gacgggggga ggcggtagt                              99


<210>  38
<211>  33
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 2 scFv-scFv

<400>  38

Arg Arg Ala Asp Ala Ala Ala Ala Gly Gly Gly Gly Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Thr Gly Gly Gly Gly 
            20                  25                  30          


Ser 
    


<210>  39
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 3 VHH-scFv cleavable

<400>  39
ggaggtggtt ctttggaagt tctgtttcaa gggcctggat ctggatct                    48


<210>  40
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 3 VHH-scFv cleavable

<400>  40

Gly Gly Gly Ser Leu Glu Val Leu Phe Gln Gly Pro Gly Ser Gly Ser 
1               5                   10                  15      


<210>  41
<211>  72
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 4 VHH-scFv extended

<400>  41
ggttctggtt ctggaggtgg ttctttggaa gttctgtttc aagggcctgg aggttcttca       60

ggatcaggat ct                                                           72


<210>  42
<211>  24
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 4 VHH-scFv extended

<400>  42

Gly Ser Gly Ser Gly Gly Gly Ser Leu Glu Val Leu Phe Gln Gly Pro 
1               5                   10                  15      


Gly Gly Ser Ser Gly Ser Gly Ser 
            20                  


<210>  43
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 5 VHH-scFv non-cleavable

<400>  43
ggaggtggag gcacaggcgg atct                                              24


<210>  44
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 5 VHH-scFv non-cleavable

<400>  44

Gly Gly Gly Gly Thr Gly Gly Ser 
1               5               


<210>  45
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 6 scFv-scFv

<400>  45
ggcggcagca ccagaagctc tagttctggt ggaggaggat ctggcggagg cgga             54


<210>  46
<211>  18
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 6 scFv-scFv

<400>  46

Gly Gly Ser Thr Arg Ser Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Gly 
        


<210>  47
<211>  51
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 7 VHH-VHH

<400>  47
ggaagcgagc ccaagattcc ccagcctcag cctaaacctc agccgcagcc t                51


<210>  48
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 7 VHH-VHH

<400>  48

Gly Ser Glu Pro Lys Ile Pro Gln Pro Gln Pro Lys Pro Gln Pro Gln 
1               5                   10                  15      


Pro 
    


<210>  49
<211>  39
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 8 VHH-VHH-scFv

<400>  49
ggtggagggc gcgccctggt gccgcgcggc agctctgga                              39


<210>  50
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 8 VHH-VHH-scFv

<400>  50

Gly Gly Gly Arg Ala Leu Val Pro Arg Gly Ser Ser Gly 
1               5                   10              


<210>  51
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Linker 9 VHH-VHH-scFv

<400>  51
ggaggtggtt ctttggaagt tctgtttcaa gggcctggat ctggatct                    48


<210>  52
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker 9 VHH-VHH-scFv

<400>  52

Gly Gly Gly Ser Leu Glu Val Leu Phe Gln Gly Pro Gly Ser Gly Ser 
1               5                   10                  15      


<210>  53
<211>  1206
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CD45-CD28 mRIPR-CD28

<400>  53
caggtgcaac tggttgaaag cggcggagga ctggtgcacc ctggcgattc tctgagactg       60

tcttgtgccg ccagcggcag cgtgttcaat tctgccacaa tgggctggta cagacagagc      120

cctggcagcc agagagagct ggtcgctaca atcgttgtgg gcacccctac ctacgccgac      180

tctgtgaaag gcagattcac catcagccgg gacaacgcca agaacatcgt gtacctgcag      240

atgaacagcc tgaagcctga agacaccgcc gtgtactact gcaactacag agccacctac      300

accagcggct acagcagaga ttattggggc cagggcaccc aagtgaccgt gtctagcgga      360

ggtggttctt tggaagttct gtttcaaggg cctggatctg gatctgacat cgtgatgaca      420

cagagcccta gcagcctggc cgtgtctgcc ggcgagaaag tgaccatgag ctgcagaagc      480

agccagagcc tgtactacag cggcatcaag aagaacctgc tggcctggta tcagcagaag      540

cccggccagt ctcctaagct gctgatctac ttcaccagca ccagactgcc tggcgtgccc      600

gatagattca caggatctgg cagcggcacc gactacaccc tgacaatcac atctgtgcag      660

gccgaggatg agggcgacta cttctgccag caaggcatca gcacccctct gacatttggc      720

ggaggcacca aactggagat caaaggcggc agcaccagaa gctctagttc tggtggagga      780

ggatctggcg gaggcggaca ggtgcagctg aaacagtctg gagccgaact tgtgaaacct      840

ggcgcctctg tgaagatcag ctgcaaaacc agcggctaca ccttcaccga cggctacatg      900

aactgggtca gacaggcccc tggacagggc ctcgagtgga tcggcagaat cgatcctgat      960

agcggcaaca cccggtacaa ccagaagttc cagggaaaag ccacactgac ccgggacaag     1020

tctagcagca ccgtgtacat ggacctgaga agcctgacca gcgaggacag cgccgtgtac     1080

tactgtgcca gagatggcac cttctacggc acctacggtt actggtactt cgacttttgg     1140

ggtcagggca cccaagtgac cgttagctct gcggccgcgc atcaccacca ccatcaccac     1200

cattaa                                                                1206


<210>  54
<211>  401
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  CD45-CD28 mRIPR-CD28

<400>  54

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val His Pro Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Val Phe Asn Ser Ala 
            20                  25                  30          


Thr Met Gly Trp Tyr Arg Gln Ser Pro Gly Ser Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Val Val Gly Thr Pro Thr Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ile Val Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn Tyr 
                85                  90                  95      


Arg Ala Thr Tyr Thr Ser Gly Tyr Ser Arg Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser Gly Gly Gly Ser Leu Glu Val Leu Phe 
        115                 120                 125             


Gln Gly Pro Gly Ser Gly Ser Asp Ile Val Met Thr Gln Ser Pro Ser 
    130                 135                 140                 


Ser Leu Ala Val Ser Ala Gly Glu Lys Val Thr Met Ser Cys Arg Ser 
145                 150                 155                 160 


Ser Gln Ser Leu Tyr Tyr Ser Gly Ile Lys Lys Asn Leu Leu Ala Trp 
                165                 170                 175     


Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Phe Thr 
            180                 185                 190         


Ser Thr Arg Leu Pro Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Asp Tyr Thr Leu Thr Ile Thr Ser Val Gln Ala Glu Asp Glu 
    210                 215                 220                 


Gly Asp Tyr Phe Cys Gln Gln Gly Ile Ser Thr Pro Leu Thr Phe Gly 
225                 230                 235                 240 


Gly Gly Thr Lys Leu Glu Ile Lys Gly Gly Ser Thr Arg Ser Ser Ser 
                245                 250                 255     


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gln Val Gln Leu Lys Gln 
            260                 265                 270         


Ser Gly Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys 
        275                 280                 285             


Lys Thr Ser Gly Tyr Thr Phe Thr Asp Gly Tyr Met Asn Trp Val Arg 
    290                 295                 300                 


Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile Gly Arg Ile Asp Pro Asp 
305                 310                 315                 320 


Ser Gly Asn Thr Arg Tyr Asn Gln Lys Phe Gln Gly Lys Ala Thr Leu 
                325                 330                 335     


Thr Arg Asp Lys Ser Ser Ser Thr Val Tyr Met Asp Leu Arg Ser Leu 
            340                 345                 350         


Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Asp Gly Thr Phe 
        355                 360                 365             


Tyr Gly Thr Tyr Gly Tyr Trp Tyr Phe Asp Phe Trp Gly Gln Gly Thr 
    370                 375                 380                 


Gln Val Thr Val Ser Ser Ala Ala Ala His His His His His His His 
385                 390                 395                 400 


His 
    


