                         SEQUENCE LISTING

<110>  Yale University
 
<120>  Blockade of miR466l-3p binding to IL-17A mRNA with site-specific 
       target site blocker prevents neuro-inflammatory-mediated disease

<130>  047162-7166WO1(00908)

<150>  62/667,763
<151>  2018-05-07

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  5452
<212>  DNA
<213>  homo sapien

<400>  1
aacagaaaat ctcgtgtctc ttgaacctag ttatttattc cttgagcaga gtagatattc       60

aacaaaagaa ttgttaaatt caattaaata ggatatatct tattattaaa tatttttttc      120

attttttgtt tacttatatg atgggaactt gagtagtttc cggaattgtc tccacaacac      180

ctggccaagg aatctgtgag gaaaagaaag atcaaatgga aaatcaaggt acatgacacc      240

agaagaccta catgttactt caaacttttt cttcctcatg aaccattaaa atagagcata      300

actcttctgg cagctgtaca tatgttcata aatacatgat attgacccat agcatagcag      360

ctctgctcag cttctaacaa gtaagaatga aaagaggaca tggtctttag gaacatgaat      420

ttctgccctt cccattttcc ttcagaagga gagattcttc tatgacctca ttgggggcgg      480

aaattttaac caaaatggtg tcacccctga acccactgcg acacgccacg taagtgacca      540

cagaaggaga aaagccctat aaaaagagag acgatagcgc tacattttgt ccatctcata      600

gcaggcacaa actcatccat ccccagttga ttggaagaaa caacgatgac tcctgggaag      660

acctcattgg tggtgagtcc tgcactaacg tgcgatgctc ttgctgattt ggaccagata      720

gtatttctgg accgtgggca tgaaacgctg ggttctgact atggagatcc aggaatactg      780

tatatgtagg ataggaaatg aaagctttgg taggtattta agtcattgtg cagcattttc      840

aagaactgat acacagcagt ttgaaagata agattaaaac tgaaagatag ctatattggg      900

gctaaaccac acaagaagtg tcacatgatg ctgtgcagta agaaagaaaa tttattgaaa      960

gtctgttttt ctgagtacaa aggatttaat ataattctcc cacggcattt ttctttaaaa     1020

tgggtcacta tccttgagat tttgaaagcc gtagcagcaa caacctttgt ttccattatc     1080

tcgtaccata ttctcagtac attgaaacta tgtattctaa ctaaacatag gtataactgt     1140

gttttagaat aagtggggtt tatatttttt aaatatttaa cttcaagtat cttttttgaa     1200

atctgatttt attacagaat caatacatgt taaatttaga acaactggaa aatataccta     1260

agaaaacatg aaggagatcg agtttttagt tggatgcctg ccagtagcac caacagcact     1320

tctagcatga atattgatac cacatagatt ttctatagct ctttcttcca atgtgaatgt     1380

ttgacttcac gatgagtttc acagaatatg ggactgagaa caatggtgca ggaggatatt     1440

tctacctaga aaatcaaggt tattattcct tcccagacct gacaatgatg catgtgctga     1500

taggctaatg acatgccatg acttgacatt tttattaaaa ttattgccaa ccaatggata     1560

acatgtcttt cctaagtcaa aaggagaatg ttgaaactag tttttttaaa aaaattttaa     1620

agccatggtg ttaacattat gttggtcatc tacctagatt tttctctagc tgatctgaaa     1680

aatgtagtat agattgtcct ggaacattgt gtgttctcta tgattagcaa tgcatcatca     1740

tcacaattaa tttgtcaaaa agaaccacat agtaatctaa tctccaacct ctctctcctt     1800

tcccattcaa ttctagtcac tgctactgct gctgagcctg gaggccatag tgaaggcagg     1860

aatcacaatc ccacgaaatc caggatgccc aaattctgag gacaagaact tcccccggac     1920

tgtgatggtc aacctgaaca tccataaccg gaataccaat accaatccca aaaggtcctc     1980

agattactac aaccgatcca cctcaccttg gaatctccag tacgtaaagc ttccagataa     2040

aaatgctata ttcttcatcc ctcttatgca tcagactgcc agttaaatct ccctgaggat     2100

gattttattc atttagaatt accagtcaaa cctggaagga ccactgtgaa gagcaattct     2160

caaactttct acagatttct ttaaccaagc acaggacagc ctccaataat ccctatcctg     2220

ttagatctaa ttgtcactga caccaataat caacccaaat taattataat cattattcta     2280

atatttatga gaccccaagt ctattcttta tttattcaaa gaatagacat ttatcaaaga     2340

ggattaatgc ttttattatc ttaaccagag ctgccattga gaagatttat tgcaaataat     2400

taataattag ggttttttac ttttattctt ttgcttattt ttgtttttga atcccagtgg     2460

aataagtatc actggggtat ttctacccct ttgtgtgtta aatagtcttg atctacttcc     2520

taacatacct atgcttgctg tatccttagt atacccagta tttagacccc atcaagggtt     2580

aaataccaaa tgtattttga tcatttgact tcatacaaat aagtctctgt tctgtggagc     2640

ctacagattg gtctgattgt aggatttctt ctcttcttcc cattactagg aagagtcaaa     2700

ataaatcaat tcaaaaatgc aagcaaatca ttcactgatc taaaagagag agggaagaga     2760

aggtcataga gacacttaac cttttgtttc cagcccttta tctcagctct gggctctgtc     2820

ccacgaatgt gatctcagat aaaattttga tgtattccct cttcaaagac agacttcatc     2880

aagtcaaata aacagctatc ttattctaga tggttccaag tctactcttc ctttggtctt     2940

cttctgtctg tcaaatgtac cctaaaaaag ctatcatttg tgtcaaactt aaattttttc     3000

tgtggcctca gtctatctta ttttattcat tcttcaaata aattggagaa aaactgatca     3060

ctgtcttctt ttctataaca attcacgtgc ttgaaaaaaa aatccaattt gtccccaaag     3120

ttcttcttca aactaacatc atttaaagaa tttgcaatgc ctataatttg tcatcctgtg     3180

aacttgcctc tcttcatgta ttcctgtttt atttctttcc cactttacca ggaattcact     3240

ttcctcctga tttttctccc ctctgcagcc gcaatgagga ccctgagaga tatccctctg     3300

tgatctggga ggcaaagtgc cgccacttgg gctgcatcaa cgctgatggg aacgtggact     3360

accacatgaa ctctgtcccc atccagcaag agatcctggt cctgcgcagg gagcctccac     3420

actgccccaa ctccttccgg ctggagaaga tactggtgtc cgtgggctgc acctgtgtca     3480

ccccgattgt ccaccatgtg gcctaagagc tctggggagc ccacactccc caaagcagtt     3540

agactatgga gagccgaccc agcccctcag gaaccctcat ccttcaaaga cagcctcatt     3600

tcggactaaa ctcattagag ttcttaaggc agtttgtcca attaaagctt cagaggtaac     3660

acttggccaa gatatgagat ctgaattacc tttccctctt tccaagaagg aaggtttgac     3720

tgagtaccaa tttgcttctt gtttactttt ttaagggctt taagttattt atgtatttaa     3780

tatgccctga gataactttg gggtataaga ttccatttta atgaattacc tactttattt     3840

tgtttgtctt tttaaagaag ataagattct gggcttggga attttattat ttaaaaggta     3900

aaacctgtat ttatttgagc tatttaagga tctatttatg tttaagtatt tagaaaaagg     3960

tgaaaaagca ctattatcag ttctgcctag gtaaatgtaa gatagaatta aatggcagtg     4020

caaaatttct gagtctttac aacatacgga tatagtattt cctcctcttt gtttttaaaa     4080

gttataacat ggctgaaaag aaagattaaa cctactttca tatgtattaa tttaaatttt     4140

gcaatttgtt gaggttttac aagagataca gcaagtctaa ctctctgttc cattaaaccc     4200

ttataataaa atccttctgt aataataaag tttcaaaaga aaatgtttat ttgttctcat     4260

taaatgtatt ttagcaaact cagctcttcc ctattgggaa gagttatgca aattctccta     4320

taagcaaaac aaagcatgtc tttgagtaac aatgacctgg aaatacccaa aattccaagt     4380

tctcgatttc acatgccttc aagactgaac accgactaag gttttcatac tattagccaa     4440

tgctgtagac agaagcattt tgataggaat agagcaaata agataatggc cctgaggaat     4500

ggcatgtcat tattaaagat catatgggga aaatgaaacc ctccccaaaa tacaagaagt     4560

tctgggagga gacattgtct tcagactaca atgtccagtt tctcccctag actcaggctt     4620

cctttggaga ttaaggcccc tcagagatca acagaccaac atttttctct tcctcaagca     4680

acactcctag ggcctggctt ctgtctgatc aaggcaccac acaacccaga aaggagctga     4740

tggggcagaa cgaactttaa gtatgagaaa agttcagccc aagtaaaata aaaactcaat     4800

cacattcaat tccagagtag tttcaagttt cacatcgtaa ccattttcgc ccccatggcc     4860

catgtgctgt cttgccctac ttctgaaggc ctctagatat tctcaggcca ctctgcaggc     4920

tccctgcttc ttgaaagacc ttcctcttca ctccatcctg ctccatccag tgtgctccag     4980

cccacccaga ggcccatggc ttttctaggc ttctttccct attccaaatc caggggttgg     5040

cctctccagc cacctcctct cagtcatttt gtgctcacat cttgattcct gtgaccatcc     5100

ctgtcaccat tttcctacag gaaatcccca ggcagcttcc agggacagtt cctcacagcc     5160

tttacgcttt caagcttctc actaaaaacc tgctgctcgt tttcaagctt tctctgaacg     5220

gtgatgatga aaagaaaaaa ttgtttttgt ttttgttggg agagggagtt gtaaaggatg     5280

tatatgtcta tatgtgtgtg tgtgtgtgtg tgtgtggtcc tgaaccatta tttcccacta     5340

gattttgggt ggggggtatg cggatgggaa gaggcagttg ctttccatgt actcctcccc     5400

tgatacactt tgcttttatt ctctcttcat tttccccagt tagcactacc at             5452


<210>  2
<211>  27
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Target site blocker

<400>  2
ccuagauaaa uacaaauaca aauucaa                                           27


<210>  3
<211>  155
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Thr Pro Gly Lys Thr Ser Leu Val Ser Leu Leu Leu Leu Leu Ser 
1               5                   10                  15      


Leu Glu Ala Ile Val Lys Ala Gly Ile Thr Ile Pro Arg Asn Pro Gly 
            20                  25                  30          


Cys Pro Asn Ser Glu Asp Lys Asn Phe Pro Arg Thr Val Met Val Asn 
        35                  40                  45              


Leu Asn Ile His Asn Arg Asn Thr Asn Thr Asn Pro Lys Arg Ser Ser 
    50                  55                  60                  


Asp Tyr Tyr Asn Arg Ser Thr Ser Pro Trp Asn Leu His Arg Asn Glu 
65                  70                  75                  80  


Asp Pro Glu Arg Tyr Pro Ser Val Ile Trp Glu Ala Lys Cys Arg His 
                85                  90                  95      


Leu Gly Cys Ile Asn Ala Asp Gly Asn Val Asp Tyr His Met Asn Ser 
            100                 105                 110         


Val Pro Ile Gln Gln Glu Ile Leu Val Leu Arg Arg Glu Pro Pro His 
        115                 120                 125             


Cys Pro Asn Ser Phe Arg Leu Glu Lys Ile Leu Val Ser Val Gly Cys 
    130                 135                 140                 


Thr Cys Val Thr Pro Ile Val His His Val Ala 
145                 150                 155 


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target site blocker

<400>  4
acttaaacat aaatagatcc t                                                 21


<210>  5
<211>  44
<212>  RNA
<213>  Homo sapiens

<400>  5
auuuauuuga gcuauuuaag gaucuauuua uguuuaagua uuua                        44


