                         SEQUENCE LISTING

<110>  Pioneer Hi-Bred International, Inc.
 
<120>  METHODS AND COMPOSITIONS FOR HOMOLOGY-DIRECTED REPAIR OF CAS 
       ENDONUCLEASE MEDIATED DOUBLE STRAND BREAKS

<130>  7664-WO-PCT

<150>  62/667968
<151>  2018-05-07

<150>  62/751845
<151>  2018-10-29

<160>  78    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  DNA
<213>  Zea mays

<400>  1
gccttcacct atcccacat                                                    19


<210>  2
<211>  20
<212>  DNA
<213>  Zea mays

<400>  2
acttgttcgg gagttaggtc                                                   20


<210>  3
<211>  19
<212>  DNA
<213>  Zea mays

<400>  3
ggtgtccatg tcgtgctca                                                    19


<210>  4
<211>  21
<212>  DNA
<213>  Zea mays

<400>  4
gcataatgag gatcgaggat g                                                 21


<210>  5
<211>  66
<212>  DNA
<213>  Zea mays

<400>  5
acattttact cttagctcat cacctatgtg ggataggtga aggcgtgaag cactccgagt       60

cttctt                                                                  66


<210>  6
<211>  67
<212>  DNA
<213>  Zea mays

<400>  6
acattttact cttagctcat cacctatgtt gggataggtg aaggcgtgaa gcactccgag       60

tcttctt                                                                 67


<210>  7
<211>  64
<212>  DNA
<213>  Zea mays

<400>  7
acattttact cttagctcat cacctatggg ataggtgaag gcgtgaagca ctccgagtct       60

tctt                                                                    64


<210>  8
<211>  65
<212>  DNA
<213>  Zea mays

<400>  8
acattttact cttagctcat cacctatggg gataggtgaa ggcgtgaagc actccgagtc       60

ttctt                                                                   65


<210>  9
<211>  67
<212>  DNA
<213>  Zea mays

<400>  9
acattttact cttagctcat cacctatgat gggataggtg aaggcgtgaa gcactccgag       60

tcttctt                                                                 67


<210>  10
<211>  67
<212>  DNA
<213>  Zea mays

<400>  10
acattttact cttagctcat cacctatggt gggataggtg aaggcgtgaa gcactccgag       60

tcttctt                                                                 67


<210>  11
<211>  67
<212>  DNA
<213>  Zea mays

<400>  11
aaggaacaca aaggctaagc ctcctacttg ttcgggagtt aggtcaggga caccatatga       60

atgaaag                                                                 67


<210>  12
<211>  66
<212>  DNA
<213>  Zea mays

<400>  12
aaggaacaca aaggctaagc ctcctacttg ttcgggagtt agtcagggac accatatgaa       60

tgaaag                                                                  66


<210>  13
<211>  64
<212>  DNA
<213>  Zea mays

<400>  13
aaggaacaca aaggctaagc ctcctacttg ttcgggagtg tcagggacac catatgaatg       60

aaag                                                                    64


<210>  14
<211>  68
<212>  DNA
<213>  Zea mays

<400>  14
aaggaacaca aaggctaagc ctcctacttg ttcgggagtt agtgtcaggg acaccatatg       60

aatgaaag                                                                68


<210>  15
<211>  65
<212>  DNA
<213>  Zea mays

<400>  15
aaggaacaca aaggctaagc ctcctacttg ttcgggagtt gtcagggaca ccatatgaat       60

gaaag                                                                   65


<210>  16
<211>  68
<212>  DNA
<213>  Zea mays

<400>  16
aaggaacaca aaggctaagc ctcctacttg ttcgggagtt agggtcaggg acaccatatg       60

aatgaaag                                                                68


<210>  17
<211>  68
<212>  DNA
<213>  Zea mays

<400>  17
gctcaggtat gatccagaaa caagcggtgt ccatgtcgtg ctcaaggggc tggtgttccc       60

aaacggcg                                                                68


<210>  18
<211>  69
<212>  DNA
<213>  Zea mays

<400>  18
gctcaggtat gatccagaaa caagcggtgt ccatgtcgtg cctcaagggg ctggtgttcc       60

caaacggcg                                                               69


<210>  19
<211>  69
<212>  DNA
<213>  Zea mays

<400>  19
gctcaggtat gatccagaaa caagcggtgt ccatgtcgtg cttcaagggg ctggtgttcc       60

caaacggcg                                                               69


<210>  20
<211>  68
<212>  DNA
<213>  Zea mays

<400>  20
gctcaggtat gatccagaaa caagcggtgt ccatgtcgtg ctcaaggggc tggtgttccc       60

aaacggcg                                                                68


<210>  21
<211>  69
<212>  DNA
<213>  Zea mays

<400>  21
gctcaggtat gatccagaaa caagcggtgt ccatgtcgtg catcaagggg ctggtgttcc       60

caaacggcg                                                               69


<210>  22
<211>  66
<212>  DNA
<213>  Zea mays

<400>  22
gctcaggtat gatccagaaa caagcggtgt ccatgtcgtg caaggggctg gtgttcccaa       60

acggcg                                                                  66


<210>  23
<211>  69
<212>  DNA
<213>  Zea mays

<400>  23
gcatcatgtc ttgaacaatg attgctcctc atcctcgatc ctcattatgc accatggacc       60

actatctaa                                                               69


<210>  24
<211>  68
<212>  DNA
<213>  Zea mays

<400>  24
gcatcatgtc ttgaacaatg attgctcctc atctcgatcc tcattatgca ccatggacca       60

ctatctaa                                                                68


<210>  25
<211>  56
<212>  DNA
<213>  Zea mays

<400>  25
gcatcatgtc ttgaacaatg attgctcctc attatgcacc atggaccact atctaa           56


<210>  26
<211>  70
<212>  DNA
<213>  Zea mays

<400>  26
gcatcatgtc ttgaacaatg attgctcctc atccctcgat cctcattatg caccatggac       60

cactatctaa                                                              70


<210>  27
<211>  66
<212>  DNA
<213>  Zea mays

<400>  27
gcatcatgtc ttgaacaatg attgctcctc atcgatcctc attatgcacc atggaccact       60

atctaa                                                                  66


<210>  28
<211>  64
<212>  DNA
<213>  Zea mays

<400>  28
gcatcatgtc ttgaacaatg attgctcctc atatcctcat tatgcaccat ggaccactat       60

ctaa                                                                    64


<210>  29
<211>  20
<212>  DNA
<213>  Zea mays

<400>  29
gccttcacct atcccaacat                                                   20


<210>  30
<211>  19
<212>  DNA
<213>  Zea mays

<400>  30
acgccttcac ctatcccat                                                    19


<210>  31
<211>  20
<212>  DNA
<213>  Zea mays

<400>  31
tacttgttcg ggagttagtc                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Zea mays

<400>  32
cctacttgtt cgggagtgtc                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Zea mays

<400>  33
ggtgtccatg tcgtgcctca                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Zea mays

<400>  34
ggtgtccatg tcgtgcttca                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Zea mays

<400>  35
gcataatgag gatcgagatg                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Zea mays

<400>  36
gtggtccatg gtgcataatg                                                   20


<210>  37
<211>  149
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-BLAT2-BN1m Sense

<400>  37
attcatagac ttgaacacaa accacacgaa tctttccttc accaggataa tgaggtaatg       60

gctcctgcag gggaaggtcc aagagcgggc gaggtagagg tgttcgcgaa aatgccgggc      120

ttcaacgaca acgtgcgcta aaacggcag                                        149


<210>  38
<211>  149
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-BLAT2-BN1m AntiSense repair template

<400>  38
ctgccgtttt agcgcacgtt gtcgttgaag cccggcattt tcgcgaacac ctctacctcg       60

cccgctcttg gaccttcccc tgcaggagcc attacctcat tatcctggtg aaggaaagat      120

tcgtgtggtt tgtgttcaag tctatgaat                                        149


<210>  39
<211>  108
<212>  DNA
<213>  Artificial

<220>
<223>  M16-BN-S repair template

<400>  39
gagcattctc taatttagtt tttttctgta tctaccatgg cggaagtcct gcaggggatg       60

gtttatgatt aggatgatta tacaggagta ccttgtacta tctttcta                   108


<210>  40
<211>  108
<212>  DNA
<213>  Artificial

<220>
<223>  M16-BN-AS repair template

<400>  40
tagaaagata gtacaaggta ctcctgtata atcatcctaa tcataaacca tcccctgcag       60

gacttccgcc atggtagata cagaaaaaaa ctaaattaga gaatgctc                   108


<210>  41
<211>  145
<212>  DNA
<213>  Artificial

<220>
<223>  NLB-CR8-BN-S repair template

<400>  41
ttccctttca cttaaagaca gactaaatac ccgggcactc ctcagacagc gagctatgcc       60

gctggattca tacacctgtg acaactgtat cttacaagcc gaagcctgca ggagacagtc      120

cttcatcttt ttttcagatg cagct                                            145


<210>  42
<211>  145
<212>  DNA
<213>  Artificial

<220>
<223>  NLB-CR8-BN-AS repair template

<400>  42
agctgcatct gaaaaaaaga tgaaggactg tctcctgcag gcttcggctt gtaagataca       60

gttgtcacag gtgtatgaat ccagcggcat agctcgctgt ctgaggagtg cccgggtatt      120

tagtctgtct ttaagtgaaa gggaa                                            145


<210>  43
<211>  98
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-Sa-BN-S repair template

<400>  43
tccgtcgcga gggaagccga cggggacccc atccggttcg cgaacgacct cgatgtgcct       60

gcaggcacag gaatggatcc gtattcttca ctgacacg                               98


<210>  44
<211>  98
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-Sa-BN-AS repair template

<400>  44
cgtgtcagtg aagaatacgg atccattcct gtgcctgcag gcacatcgag gtcgttcgcg       60

aaccggatgg ggtccccgtc ggcttccctc gcgacgga                               98


<210>  45
<211>  98
<212>  DNA
<213>  Artificial

<220>
<223>  TS50-BN-S repair template

<400>  45
ggtagcatgt gtgaatccca tttctcctag aaccacactg aacaacctgc aggcaacggc       60

agagttgttt gtatctagat ctcgaccgat ccttcacc                               98


<210>  46
<211>  98
<212>  DNA
<213>  Artificial

<220>
<223>  TS50-BN-AS repair template

<400>  46
ggtgaaggat cggtcgagat ctagatacaa acaactctgc cgttgcctgc aggttgttca       60

gtgtggttct aggagaaatg ggattcacac atgctacc                               98


<210>  47
<211>  178
<212>  DNA
<213>  Artificial

<220>
<223>  TS45_Sense repair template

<400>  47
atctggatcc tgaaatcggc gtcgtaacct acaaggccac ggactggatt agatagtggt       60

ccatggtgca taatgaggat ggaggcctgc aggatgaaga gcaatcattg ttcaagacat      120

gatgcaaagc tagaaaactt tgattgtggc cgtcctaatt gtgaagttta ggccgggg        178


<210>  48
<211>  178
<212>  DNA
<213>  Artificial

<220>
<223>  TS45_Antisense repair template

<400>  48
ccccggccta aacttcacaa ttaggacggc cacaatcaaa gttttctagc tttgcatcat       60

gtcttgaaca atgattgctc ttcatcctgc aggcctccat cctcattatg caccatggac      120

cactatctaa tccagtccgt ggccttgtag gttacgacgc cgatttcagg atccagat        178


<210>  49
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-BLAT2-Spyn1 flanking Spy Guide for MS45 BLAT target

<400>  49
gaacacaaac cacacgaatc                                                   20


<210>  50
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-BLAT2-Spyn2 flanking Spy Guide for MS45 BLAT target

<400>  50
gttgtcgggg aagcccggc                                                    19


<210>  51
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-BLAT2-Spyn3 flanking Spy Guide for MS45 BLAT target

<400>  51
gccgttggag cgcacgttgt c                                                 21


<210>  52
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-BLAT2-Spyn4 flanking Spy Guide for MS45 BLAT target

<400>  52
gaactggccc ctgccgt                                                      17


<210>  53
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-BLAT2-Spyn5 flanking Spy Guide for MS45 BLAT target

<400>  53
gtccgagaca acaaactgc                                                    19


<210>  54
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  M16-BLAT-L flanking BLAT Guide for named Spy sites

<400>  54
gtgtgtcttt gccatatgtt                                                   20


<210>  55
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  M16-BLAT-R flanking BLAT Guide for named Spy sites

<400>  55
gagaacctaa taaatttc                                                     18


<210>  56
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  NLB18_8_BLAT-L flanking BLAT Guide for named Spy sites

<400>  56
gtatccagca gtttacgcat atgg                                              24


<210>  57
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  NLB18_8_BLAT-R flanking BLAT Guide for named Spy sites

<400>  57
gtgcaagccg tagccagacg                                                   20


<210>  58
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  NLB_8_BLAT-L flanking BLAT Guide for named Spy sites

<400>  58
gcacttgtgt tggacaataa a                                                 21


<210>  59
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  NLB_8_BLAT-R flanking BLAT Guide for named Spy sites

<400>  59
ggtcctacca tcgtcttcta a                                                 21


<210>  60
<211>  17
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-Spy-L flanking Spy Guide for named Sa sites

<400>  60
gtcgccgacg cgtacta                                                      17


<210>  61
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  MS45-Spy-R flanking Spy Guide for named Sa sites

<400>  61
gctttcagca gagatacag                                                    19


<210>  62
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  MS26-Spy-L flanking Spy Guide for named Sa sites

<400>  62
gcgtgacgat gatgttgg                                                     18


<210>  63
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  MS26-Spy-R flanking Spy Guide for named Sa sites

<400>  63
gagccaggca tccaaggc                                                     18


<210>  64
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  TS50-Sa-L flanking Sa Guide for named Spy sites

<400>  64
gtgatcagag tcgtgatttg                                                   20


<210>  65
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  TS50-Sa-R flanking Sa Guide for named Spy sites

<400>  65
gtgcctggtt tcttgcacta cc                                                22


<210>  66
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  TS45-Sa-L flanking Sa Guide for named Spy sites

<400>  66
gtgagactaa tgaaaatcac at                                                22


<210>  67
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  TS45-Sa-R flanking Sa Guide for named Spy sites

<400>  67
gaaagaacta attaagcttc ga                                                22


<210>  68
<211>  526
<212>  DNA
<213>  Zea mays

<400>  68
gtttaatata atagaatcgt cgtgtagatg ctttcttatc tttatgactt cattcgattg       60

tgacaaattg tagttacgat tacttaaata tatttgctat cccattcatg ccttttccac      120

ttaaatcgca tctccatgtg tctttgccat atgtttagtc aaaattttat tactttgttg      180

atcgttttct gcgtgtatac aacacggtct gcacatatat catgagcatt ctctaattta      240

gtttttttct gtatctacca tggcggaagt ggatggttta tgattaggat gattatacag      300

gagtaccttg tactatcttt ctaaatggca gttataggaa attgagatgt atattagatt      360

atttcaaata atagtggaga taaataattt gaatggaaat ttattaggtt ctgttgacgc      420

aaaatcctgg atgaaccgga ttattgataa aaaatattgc atggtcaatg atatgtacgg      480

agtatcttag aaaatattgc ataaacggta gttttagtta gttacg                     526


<210>  69
<211>  404
<212>  DNA
<213>  Zea mays

<400>  69
tgtactttgt ccaccttttt acacttaggc acttgtgttg gacaataaat caccaaaaca       60

cttagaaatg gcccaaaggc acatttccct ttcacttaaa gacagactaa atacccgggc      120

actcctcaga cagcgagcta tgccgctgga ttcatacacc tgtgacaact gtatcttaca      180

agccgaagag acagtccttc atcttttttt cagatgcagc tttgctagga gatgttggct      240

cttaatagga atctccccgc caagaacaac tgacttaatc aacaccattt tgaggattag      300

aagacgatgg taggacccat ggagactaga aactatcatc cttttaacct ggtgcatttg      360

gaaaagcaga aataattggg tgttcattga gatcccgccc atgg                       404


<210>  70
<211>  406
<212>  DNA
<213>  Zea mays

<400>  70
cgacgaggaa gcagcacgag aaggaggagt tctgcggccg gccgctcggc ctgaggttcc       60

acggggagac cggcgagctc tacgtcgccg acgcgtacta cggtctcatg gtcgttggcc      120

agagcggcgg cgtggcgtcc tccgtcgcga gggaagccga cggggacccc atccggttcg      180

cgaacgacct cgatgtgcac aggaatggat ccgtattctt cactgacacg agcatgagat      240

acagcagaaa gtgagcaaag cgacgaaaca atccggcttc tcattttcaa acgcctctgt      300

atctctgctg aaagagtagc tcaccagaca agagctgaat ttgcagggac catctgaaca      360

tcctgttaga aggagaaggc accgggaggc tgctcaggta tgatcc                     406


<210>  71
<211>  821
<212>  DNA
<213>  Zea mays

<400>  71
gttgcattga gccattgaca tcaacatcaa atgaatccag aattgagttg ttcctatcaa       60

gaaatggtgt cataccacta gcatcgtatt gtcggtactc atcaatctgt gtgccgcttc      120

tggggctaga agtcatctca ttcaaagagc catagattca ttaagtaccg cattcctctc      180

atcgtcatca gtatctgaga tgtgaaccgt agattaagat ttgtagccaa ccttcggttc      240

tctgtatcct tcatgcggca agtgatcaga gtcgtgattt gaagaatgca caacactagg      300

catggttatg cttctttgtt ttaatttgct catatcatca tctggaaacc tcaggtagca      360

tgtgtgaatc ccatttctcc tagaaccaca ctgaacaaca acggcagagt tgtttgtatc      420

tagatctcga ccgatccttc accttatccc tggcattgat gtttgttagg ttacaggttt      480

taccacccga ggtagtgcaa gaaaccaggc acctatcaca cataccatca gagcgctcaa      540

gattgttgtg actagcaata cgacaaacgc gatatttcct acctgtgtgc agtacaaact      600

acttcgaacc aggcctacaa tctgatccaa tctcgagcac atcatacatt atgaccggag      660

gcaagtatta tgatcaatgt aacacaccaa aatcctattt ttaggattca aaaaaattat      720

ctaaaaattt aaaatttaaa aagtctttat aataaaatta taaattaaaa ttattttcct      780

aaaagttaaa taaatattaa atataaaatt ttgactacgt g                          821


<210>  72
<211>  270
<212>  DNA
<213>  Zea mays

<400>  72
ggaggtagtg catcctttgt gagactaatg aaaatcacat ctggatcctg aaatcggcgt       60

cgtaacctac aaggccacgg actggattag atagtggtcc atggtgcata atgaggatcg      120

aggatgagga gcaatcattg ttcaagacat gatgcaaagc tagaaaactt tgattgtggc      180

cgtcctaatt gtgaagttta ggccgggggg aacttcatga accctatcga agcttaatta      240

gttctttttt gttgttagcc atgtttgtat                                       270


<210>  73
<211>  267
<212>  DNA
<213>  Zea mays

<400>  73
ctccgagaca acaaactgca ggtaacaaaa atactatctg acgatgctca tgattctacc       60

gtatccatag tcatgaacac aaaccacacg aatctggcct tgaccaggat aatgaggtac      120

tggctggaag gcccaagagc gggcgaggta gaggtgttcg cgaacctgcc gggcttcccc      180

gacaacgtgc gctccaacgg caggggccag ttctgggtgg cgatcgactg ctgccggacg      240

ccggcgcagg aggtgttcgc caagagg                                          267


<210>  74
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  MS45 BLAT2 sgRNA Guide

<400>  74
gataatgagg tactggctgg a                                                 21


<210>  75
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  MS45 Sa sgRNA Guide

<400>  75
gcgaacgacc tcgatgtgca c                                                 21


<210>  76
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  TS50 sgRNA Guide

<400>  76
gaaccacact gaacaacaa                                                    19


<210>  77
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  M16 sgRNA Guide

<400>  77
gtatctacca tggcggaagt gga                                               23


<210>  78
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  NLB_8 sgRNA Guide

<400>  78
ttctacttcc tgacagagaa                                                   20


