                         SEQUENCE LISTING

<110>  SpyBiotech Limited
 
<120>  Vaccine composition

<130>  P9800wo1

<160>  60    

<170>  PatentIn version 3.5

<210>  1
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker 2 - (GSG)2

<400>  1

Gly Ser Gly Gly Ser Gly 
1               5       


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker 3 - (GSG)3

<400>  2

Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker 4 - (G4S)1

<400>  3

Gly Gly Gly Gly Ser 
1               5   


<210>  4
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker 5 - (G4S)3

<400>  4

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  5
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker 6 - (G4S)4

<400>  5

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  6
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker 7

<400>  6

Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 
1               5                   10          


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker 8

<400>  7

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  8
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker 9

<400>  8

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Ser Thr 
1               5                   10                  


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rigid linker 1

<400>  9

Glu Ala Ala Ala Lys 
1               5   


<210>  10
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rigid linker 2 - (EAAAK)3

<400>  10

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


<210>  11
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rigid linker 3 - (AP)7

<400>  11

Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro 
1               5                   10                  


<210>  12
<211>  2235
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gH-SpyTag-His


<220>
<221>  misc_feature
<222>  (1146)..(1146)
<223>  C>A mutation at position 1146

<220>
<221>  misc_feature
<222>  (1)..(69)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (70)..(2151)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (2152)..(2157)
<223>  Transmembrane domain (truncated)

<220>
<221>  misc_feature
<222>  (2158)..(2175)
<223>  Linker

<220>
<221>  misc_feature
<222>  (2176)..(2214)
<223>  SpyTag

<220>
<221>  misc_feature
<222>  (2215)..(2232)
<223>  6x His tag

<220>
<221>  misc_feature
<222>  (2233)..(2235)
<223>  Stop codon

<400>  12
atgcggccag gcctcccctc ctacctcatc atcctcgccg tctgtctctt cagccaccta       60

ctttcgtcac gatatggcgc agaagccgta tccgaaccgc tggacaaagc gtttcaccta      120

ctgctcaaca cctacgggag acccatccgc ttcctgcgtg aaaataccac ccagtgtacc      180

tacaacagca gcctccgtaa cagcacggtc gtcagggaaa acgccatcag tttcaacttt      240

ttccaaagct ataatcaata ctatgtattc catatgcctc gatgtctttt tgcgggtcct      300

ctggcggagc agtttctgaa ccaggtagat ctgaccgaaa ccctggaaag ataccaacag      360

agacttaaca cttacgcgct ggtatccaaa gacctggcca gctaccgatc tttttcgcag      420

cagctaaagg cacaagacag cctaggtgaa cagcccacca ctgtgccacc gcccattgac      480

ctgtcaatac ctcacgtttg gatgccaccg caaaccactc cacacggctg gacagaatca      540

cataccacct caggactaca ccgaccacac tttaaccaga cctgtatcct ctttgatgga      600

cacgatctac tattcagcac cgtcacacct tgtttgcacc aaggctttta cctcatcgac      660

gaactacgtt acgttaaaat aacactgacc gaggacttct tcgtagttac ggtgtccata      720

gacgacgaca cacccatgct gcttatcttc ggccatcttc cacgcgtact tttcaaagcg      780

ccctatcaac gcgacaactt tatactacga caaactgaaa aacacgagct cctggtgcta      840

gttaagaaag atcaactgaa ccgtcactct tatctcaaag acccggactt tcttgacgcc      900

gcacttgact tcaactacct agacctcagc gcactactac gtaacagctt tcaccgttac      960

gccgtggatg tactcaagag cggtcgatgt cagatgctgg accgccgcac ggtagaaatg     1020

gccttcgcct acgcattagc actgttcgca gcagcccgac aagaagaggc cggcgcccaa     1080

gtctccgtcc cacgggccct agaccgccag gccgcactct tacaaataca agaatttatg     1140

atcacatgcc tctcacaaac accaccacgc accacgttgc tgctgtatcc cacggccgtg     1200

gacctggcca aacgagccct ttggacaccg aatcagatca ccgacatcac cagcctcgta     1260

cgcctggtct acatactctc taaacagaat cagcaacatc tcatccccca atgggcacta     1320

cgacagatcg ccgactttgc cctaaaacta cacaaaacgc acctggcctc ttttctttca     1380

gccttcgcac gccaagaact ctacctcatg ggcagcctcg tccactccat gctggtacat     1440

acgacggaga gacgcgaaat cttcatcgta gaaacgggcc tctgttcatt ggccgagcta     1500

tcacacttta cgcagttgtt agctcatcca caccacgaat acctcagcga cctgtacaca     1560

ccctgttcca gtagcgggcg acgcgatcac tcgctcgaac gcctcacgcg tctcttcccc     1620

gatgccaccg tccccgctac cgttcccgcc gccctctcca tcctatctac catgcaacca     1680

agcacgctgg aaaccttccc cgacctgttt tgcttgccgc tcggcgaatc cttctccgcg     1740

ctgaccgtct ccgaacacgt cagttatatc gtaacaaacc agtacctgat caaaggtatc     1800

tcctaccctg tctccaccac cgtcgtaggc cagagcctca tcatcaccca gacggacagt     1860

caaactaaat gcgaactgac gcgcaacatg cataccacac acagcatcac agtggcgctc     1920

aacatttcgc tagaaaactg cgccttttgc caaagcgccc tgctagaata cgacgacacg     1980

caaggcgtca tcaacatcat gtacatgcac gactcggacg acgtcctttt cgccctggat     2040

ccctacaacg aagtggtggt ctcatctccg cgaactcact acctcatgct tttgaaaaac     2100

ggtacggtac tagaagtaac tgacgtcgtc gtggacgcca ccgacagtcg tctcctcgga     2160

agcggaggct ctggtgccca tatcgtgatg gtggacgcct acaagcctac caaacatcat     2220

caccatcacc actaa                                                      2235


<210>  13
<211>  2157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gH with truncated transmembrane domain

<220>
<221>  misc_feature
<222>  (1146)..(1146)
<223>  C>A mutation at position 1146

<220>
<221>  misc_feature
<222>  (1)..(69)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (70)..(2151)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (2152)..(2157)
<223>  Transmembrane domain (truncated)

<400>  13
atgcggccag gcctcccctc ctacctcatc atcctcgccg tctgtctctt cagccaccta       60

ctttcgtcac gatatggcgc agaagccgta tccgaaccgc tggacaaagc gtttcaccta      120

ctgctcaaca cctacgggag acccatccgc ttcctgcgtg aaaataccac ccagtgtacc      180

tacaacagca gcctccgtaa cagcacggtc gtcagggaaa acgccatcag tttcaacttt      240

ttccaaagct ataatcaata ctatgtattc catatgcctc gatgtctttt tgcgggtcct      300

ctggcggagc agtttctgaa ccaggtagat ctgaccgaaa ccctggaaag ataccaacag      360

agacttaaca cttacgcgct ggtatccaaa gacctggcca gctaccgatc tttttcgcag      420

cagctaaagg cacaagacag cctaggtgaa cagcccacca ctgtgccacc gcccattgac      480

ctgtcaatac ctcacgtttg gatgccaccg caaaccactc cacacggctg gacagaatca      540

cataccacct caggactaca ccgaccacac tttaaccaga cctgtatcct ctttgatgga      600

cacgatctac tattcagcac cgtcacacct tgtttgcacc aaggctttta cctcatcgac      660

gaactacgtt acgttaaaat aacactgacc gaggacttct tcgtagttac ggtgtccata      720

gacgacgaca cacccatgct gcttatcttc ggccatcttc cacgcgtact tttcaaagcg      780

ccctatcaac gcgacaactt tatactacga caaactgaaa aacacgagct cctggtgcta      840

gttaagaaag atcaactgaa ccgtcactct tatctcaaag acccggactt tcttgacgcc      900

gcacttgact tcaactacct agacctcagc gcactactac gtaacagctt tcaccgttac      960

gccgtggatg tactcaagag cggtcgatgt cagatgctgg accgccgcac ggtagaaatg     1020

gccttcgcct acgcattagc actgttcgca gcagcccgac aagaagaggc cggcgcccaa     1080

gtctccgtcc cacgggccct agaccgccag gccgcactct tacaaataca agaatttatg     1140

atcacatgcc tctcacaaac accaccacgc accacgttgc tgctgtatcc cacggccgtg     1200

gacctggcca aacgagccct ttggacaccg aatcagatca ccgacatcac cagcctcgta     1260

cgcctggtct acatactctc taaacagaat cagcaacatc tcatccccca atgggcacta     1320

cgacagatcg ccgactttgc cctaaaacta cacaaaacgc acctggcctc ttttctttca     1380

gccttcgcac gccaagaact ctacctcatg ggcagcctcg tccactccat gctggtacat     1440

acgacggaga gacgcgaaat cttcatcgta gaaacgggcc tctgttcatt ggccgagcta     1500

tcacacttta cgcagttgtt agctcatcca caccacgaat acctcagcga cctgtacaca     1560

ccctgttcca gtagcgggcg acgcgatcac tcgctcgaac gcctcacgcg tctcttcccc     1620

gatgccaccg tccccgctac cgttcccgcc gccctctcca tcctatctac catgcaacca     1680

agcacgctgg aaaccttccc cgacctgttt tgcttgccgc tcggcgaatc cttctccgcg     1740

ctgaccgtct ccgaacacgt cagttatatc gtaacaaacc agtacctgat caaaggtatc     1800

tcctaccctg tctccaccac cgtcgtaggc cagagcctca tcatcaccca gacggacagt     1860

caaactaaat gcgaactgac gcgcaacatg cataccacac acagcatcac agtggcgctc     1920

aacatttcgc tagaaaactg cgccttttgc caaagcgccc tgctagaata cgacgacacg     1980

caaggcgtca tcaacatcat gtacatgcac gactcggacg acgtcctttt cgccctggat     2040

ccctacaacg aagtggtggt ctcatctccg cgaactcact acctcatgct tttgaaaaac     2100

ggtacggtac tagaagtaac tgacgtcgtc gtggacgcca ccgacagtcg tctcctc        2157


<210>  14
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker from gH construct

<400>  14
ggaagcggag gctctggt                                                     18


<210>  15
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spytag

<400>  15
gcccatatcg tgatggtgga cgcctacaag cctaccaaa                              39


<210>  16
<211>  837
<212>  DNA
<213>  Human cytomegalovirus


<220>
<221>  misc_feature
<222>  (1)..(90)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (91)..(834)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (835)..(837)
<223>  Stop codon

<400>  16
atgtgccgcc gcccggattg cggcttctct ttctcacctg gaccggtgat actgctgtgg       60

tgttgccttc tgctgcccat tgtttcctca gccgccgtca gcgtcgctcc taccgccgcc      120

gagaaagtcc ccgcggagtg ccccgaacta acgcgccgat gcttgttggg tgaggtgttt      180

gagggtgaca agtatgaaag ttggctgcgc ccgttggtga atgttaccgg gcgcgatggc      240

ccgctatcgc aacttatccg ttaccgtccc gttacgccgg aggccgccaa ctccgtgctg      300

ttggacgagg ctttcctgga cactctggcc ctgctgtaca acaatccgga tcaattgcgg      360

gccctgctga cgctgttgag ctcggacaca gcgccgcgct ggatgacggt gatgcgcggc      420

tacagcgagt gcggcgatgg ctcgccggcc gtgtacacgt gcgtggacga cctgtgccgc      480

ggctacgacc tcacgcgact gtcatacggg cgcagcatct tcacggaaca cgtgttaggc      540

ttcgagctgg tgccaccgtc tctctttaac gtggtggtgg ccatacgcaa cgaagccacg      600

cgtaccaacc gcgccgtgcg tctgcccgtg agcaccgctg ccgcgcccga gggcatcacg      660

ctcttttacg gcctgtacaa cgcagtgaag gaattctgcc tgcgtcacca gctggacccg      720

ccgctgctac gccacctaga taaatactac gccggactgc cgcccgagct gaagcagacg      780

cgcgtcaacc tgccggctca ctcgcgctat ggccctcaag cagtggatgc tcgctaa         837


<210>  17
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UL130 - C-tag


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (76)..(642)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (643)..(687)
<223>  Linker

<220>
<221>  misc_feature
<222>  (688)..(699)
<223>  C-tag

<220>
<221>  misc_feature
<222>  (700)..(702)
<223>  Stop codon

<400>  17
atgctgcggc ttctgcttcg tcaccacttt cactgcctgc ttctgtgcgc ggtttgggca       60

acgccctgtc tggcgtctcc gtggtcgacg ctaacagcaa accagaatcc gtccccgcca      120

tggtctaaac tgacgtattc caaaccgcat gacgcggcga cgttttactg tccttttctc      180

tatccctcgc ccccacgatc ccccttgcaa ttctcggggt tccagcgggt atcaacgggt      240

cccgagtgtc gcaacgagac cctgtatctg ctgtacaacc gggaaggcca gaccttggtg      300

gagagaagct ccacctgggt gaaaaaggtg atctggtacc tgagcggtcg gaaccaaacc      360

atcctccaac ggatgccccg aacggcttcg aaaccgagcg acggaaacgt gcagatcagc      420

gtggaagacg ccaagatttt tggagcgcac atggtgccca agcagaccaa gctgctacgc      480

ttcgtcgtca acgatggcac acgttatcag atgtgtgtga tgaagctgga gagctgggct      540

cacgtcttcc gggactacag cgtgtctttt caggtgcgat tgacgttcac cgaggccaat      600

aaccagactt acaccttctg cacccatccc aatctcatcg ttggaggcgg aggatctggc      660

ggaggtggaa gtggcggagg cggatctgag cccgaggcct aa                         702


<210>  18
<211>  642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UL130 (signal sequence and ectodomain)


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (76)..(642)
<223>  Ectodomain

<400>  18
atgctgcggc ttctgcttcg tcaccacttt cactgcctgc ttctgtgcgc ggtttgggca       60

acgccctgtc tggcgtctcc gtggtcgacg ctaacagcaa accagaatcc gtccccgcca      120

tggtctaaac tgacgtattc caaaccgcat gacgcggcga cgttttactg tccttttctc      180

tatccctcgc ccccacgatc ccccttgcaa ttctcggggt tccagcgggt atcaacgggt      240

cccgagtgtc gcaacgagac cctgtatctg ctgtacaacc gggaaggcca gaccttggtg      300

gagagaagct ccacctgggt gaaaaaggtg atctggtacc tgagcggtcg gaaccaaacc      360

atcctccaac ggatgccccg aacggcttcg aaaccgagcg acggaaacgt gcagatcagc      420

gtggaagacg ccaagatttt tggagcgcac atggtgccca agcagaccaa gctgctacgc      480

ttcgtcgtca acgatggcac acgttatcag atgtgtgtga tgaagctgga gagctgggct      540

cacgtcttcc gggactacag cgtgtctttt caggtgcgat tgacgttcac cgaggccaat      600

aaccagactt acaccttctg cacccatccc aatctcatcg tt                         642


<210>  19
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker from UL130 construct

<400>  19
ggaggcggag gatctggcgg aggtggaagt ggcggaggcg gatct                       45


<210>  20
<211>  759
<212>  DNA
<213>  Human cytomegalovirus


<220>
<221>  misc_feature
<222>  (634)..(634)
<223>  T>C mutation at position 634

<220>
<221>  misc_feature
<222>  (1)..(81)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (82)..(164)
<223>  Ectodomain

<220>
<221>  Intron
<222>  (165)..(287)

<220>
<221>  misc_feature
<222>  (288)..(422)
<223>  Ectodomain

<220>
<221>  Intron
<222>  (423)..(542)

<220>
<221>  misc_feature
<222>  (543)..(756)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (757)..(759)
<223>  Stop codon

<400>  20
atgagtccca aagatctgac gccgttcttg acggcgttgt ggctgctatt gggtcacagc       60

cgcgtgccgc gggtgcgcgc agaagaatgt tgcgaattca taaacgtcaa ccacccgccg      120

gaacgctgtt acgatttcaa aatgtgcaat cgcttcaccg tcgcgtacgt attttcatga      180

ttgtctgcgt tctgtggtgc gtctggatct gtctctcgac gtttctgata gccatgttcc      240

atcgacgatc ctcgggaatg ccagagtaga ttttcatgaa tccacaggct gcggtgtccg      300

gacggcgaag tctgctacag tcccgagaaa acggctgaga ttcgcgggat cgtcaccacc      360

atgacccatt cattgacacg ccaggtcgta cacaacaaac tgacgagctg caactacaat      420

ccgtaagtct cttcctgagg gccttacagc ctatgggaga gtaagacaga gagggacaaa      480

acatcattaa aaaaaaaagt ctaatttcac gttttgtacc ccccttcccc tccgtgttgt      540

aggttatacc tcgaagctga cgggcgaata cgctgcggca aagtaaacga caaggcgcag      600

tacctgctgg gcgccgctgg cagcgttccc tatcgatgga tcaatctgga atacgacaag      660

ataacccgga tcgtgggcct ggatcagtac ctggagagcg ttaagaaaca caaacggctg      720

gatgtgtgcc gcgctaaaat gggctatatg ctgcagtga                             759


<210>  21
<211>  498
<212>  DNA
<213>  Human cytomegalovirus


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (55)..(236)
<223>  Ectodomain

<220>
<221>  Intron
<222>  (237)..(344)

<220>
<221>  misc_feature
<222>  (345)..(495)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (496)..(498)
<223>  Stop codon

<400>  21
atgcggctgt gtcgggtgtg gctgtctgtt tgtctgtgcg ccgtggtgct gggtcagtgc       60

cagcgggaaa ccgcggaaaa aaacgattat taccgagtac cgcattactg ggacgcgtgc      120

tctcgcgcgc tgcccgacca aacccgttac aagtatgtgg aacagctcgt ggacctcacg      180

ttgaactacc actacgatgc gagccacggc ttggacaact ttgacgtgct caagaggtga      240

gggtacgcgc taaagatgca tgacaacggg aaggtaaggg cgaacgggta acgggtaagt      300

aaccgcatgg ggtatgaaat gacgttcgga acctgtgctt gcagaatcaa cgtgaccgag      360

gtgtcgttgc tcatcagcga ctttagacgt cagaaccgtc gcggcggcac caacaaaagg      420

accacgttca acgccgccgg ttcgctggcg ccacacgccc ggagcctcga gttcagcgtg      480

cggctctttg ccaactag                                                    498


<210>  22
<211>  1008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpyCatcher-HBsAg


<220>
<221>  misc_feature
<222>  (1)..(276)
<223>  SpyCatcherDeltaN1

<220>
<221>  misc_feature
<222>  (277)..(303)
<223>  Flexible Linker

<220>
<221>  misc_feature
<222>  (304)..(315)
<223>  PVTN linker

<220>
<221>  misc_feature
<222>  (316)..(993)
<223>  HBsAg

<220>
<221>  misc_feature
<222>  (994)..(1005)
<223>  C-tag

<220>
<221>  misc_feature
<222>  (1006)..(1008)
<223>  Stop codon

<400>  22
gactccgcta ctcacatcaa gttctccaag agagatgagg acggtaaaga attggctggt       60

gctactatgg aattgagaga ctcctccggt aagactatct ccacttggat ttccgacggt      120

caggttaagg acttctactt gtacccaggt aagtacactt tcgttgagac tgctgctcca      180

gacggttacg aagttgctac tgctatcact ttcactgtta acgagcaggg acaggttaca      240

gttaacggta aggctactaa gggtgacgct catattggtt ctggtggatc tggtggttcc      300

ggtccagtta ctaatatgga aaacatcact tccggtttct tgggtccttt gttggttttg      360

caggctggat tcttcttgtt gactagaatc ttgactatcc cacagtcctt ggactcttgg      420

tggacttcct tgaacttctt gggtggttcc ccagtttgtt tgggtcaaaa ctctcaatcc      480

ccaacttcca accactcccc aacatcttgt ccaccaattt gtcctggtta cagatggatg      540

tgtttgagaa gattcatcat tttcttgttc atcttgttgt tgtgtttgat cttcttgttg      600

gttttgttgg actaccaggg tatgttgcca gtttgtccat tgatcccagg ttccactact      660

acaaacactg gtccatgtaa gacttgtact actccagctc agggtaactc catgttccct      720

tcatgttgtt gtactaagcc aactgacggt aactgtactt gtatcccaat tccatcctcc      780

tgggctttcg ctaagtactt gtgggaatgg gcttccgtta gattctcctg gttgtccttg      840

ttggttccat tcgttcagtg gttcgttggt ttgtccccaa ctgtttggtt gtccgctatt      900

tggatgatgt ggtactgggg tccatccttg tactctatcg tttccccatt catccctttg      960

ttgccaatct tcttctgttt gtgggtttac atcgagccag aggcttaa                  1008


<210>  23
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpyCatcherDeltaN1

<400>  23
gactccgcta ctcacatcaa gttctccaag agagatgagg acggtaaaga attggctggt       60

gctactatgg aattgagaga ctcctccggt aagactatct ccacttggat ttccgacggt      120

caggttaagg acttctactt gtacccaggt aagtacactt tcgttgagac tgctgctcca      180

gacggttacg aagttgctac tgctatcact ttcactgtta acgagcaggg acaggttaca      240

gttaacggta aggctactaa gggtgacgct catatt                                276


<210>  24
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Flexible linker from SpyCatcher-HBsAg

<400>  24
ggttctggtg gatctggtgg ttccggt                                           27


<210>  25
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PVTN linker from SpyCatcher-HbsAg 

<400>  25
ccagttacta at                                                           12


<210>  26
<211>  678
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBsAg

<400>  26
atggaaaaca tcacttccgg tttcttgggt cctttgttgg ttttgcaggc tggattcttc       60

ttgttgacta gaatcttgac tatcccacag tccttggact cttggtggac ttccttgaac      120

ttcttgggtg gttccccagt ttgtttgggt caaaactctc aatccccaac ttccaaccac      180

tccccaacat cttgtccacc aatttgtcct ggttacagat ggatgtgttt gagaagattc      240

atcattttct tgttcatctt gttgttgtgt ttgatcttct tgttggtttt gttggactac      300

cagggtatgt tgccagtttg tccattgatc ccaggttcca ctactacaaa cactggtcca      360

tgtaagactt gtactactcc agctcagggt aactccatgt tcccttcatg ttgttgtact      420

aagccaactg acggtaactg tacttgtatc ccaattccat cctcctgggc tttcgctaag      480

tacttgtggg aatgggcttc cgttagattc tcctggttgt ccttgttggt tccattcgtt      540

cagtggttcg ttggtttgtc cccaactgtt tggttgtccg ctatttggat gatgtggtac      600

tggggtccat ccttgtactc tatcgtttcc ccattcatcc ctttgttgcc aatcttcttc      660

tgtttgtggg tttacatc                                                    678


<210>  27
<211>  744
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gH-SpyTag-His


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (24)..(717)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (718)..(719)
<223>  Transmembrane domain (truncated)

<220>
<221>  misc_feature
<222>  (720)..(725)
<223>  Linker

<220>
<221>  misc_feature
<222>  (726)..(738)
<223>  Spytag

<220>
<221>  misc_feature
<222>  (739)..(744)
<223>  6x His tag

<400>  27

Met Arg Pro Gly Leu Pro Ser Tyr Leu Ile Ile Leu Ala Val Cys Leu 
1               5                   10                  15      


Phe Ser His Leu Leu Ser Ser Arg Tyr Gly Ala Glu Ala Val Ser Glu 
            20                  25                  30          


Pro Leu Asp Lys Ala Phe His Leu Leu Leu Asn Thr Tyr Gly Arg Pro 
        35                  40                  45              


Ile Arg Phe Leu Arg Glu Asn Thr Thr Gln Cys Thr Tyr Asn Ser Ser 
    50                  55                  60                  


Leu Arg Asn Ser Thr Val Val Arg Glu Asn Ala Ile Ser Phe Asn Phe 
65                  70                  75                  80  


Phe Gln Ser Tyr Asn Gln Tyr Tyr Val Phe His Met Pro Arg Cys Leu 
                85                  90                  95      


Phe Ala Gly Pro Leu Ala Glu Gln Phe Leu Asn Gln Val Asp Leu Thr 
            100                 105                 110         


Glu Thr Leu Glu Arg Tyr Gln Gln Arg Leu Asn Thr Tyr Ala Leu Val 
        115                 120                 125             


Ser Lys Asp Leu Ala Ser Tyr Arg Ser Phe Ser Gln Gln Leu Lys Ala 
    130                 135                 140                 


Gln Asp Ser Leu Gly Glu Gln Pro Thr Thr Val Pro Pro Pro Ile Asp 
145                 150                 155                 160 


Leu Ser Ile Pro His Val Trp Met Pro Pro Gln Thr Thr Pro His Gly 
                165                 170                 175     


Trp Thr Glu Ser His Thr Thr Ser Gly Leu His Arg Pro His Phe Asn 
            180                 185                 190         


Gln Thr Cys Ile Leu Phe Asp Gly His Asp Leu Leu Phe Ser Thr Val 
        195                 200                 205             


Thr Pro Cys Leu His Gln Gly Phe Tyr Leu Ile Asp Glu Leu Arg Tyr 
    210                 215                 220                 


Val Lys Ile Thr Leu Thr Glu Asp Phe Phe Val Val Thr Val Ser Ile 
225                 230                 235                 240 


Asp Asp Asp Thr Pro Met Leu Leu Ile Phe Gly His Leu Pro Arg Val 
                245                 250                 255     


Leu Phe Lys Ala Pro Tyr Gln Arg Asp Asn Phe Ile Leu Arg Gln Thr 
            260                 265                 270         


Glu Lys His Glu Leu Leu Val Leu Val Lys Lys Asp Gln Leu Asn Arg 
        275                 280                 285             


His Ser Tyr Leu Lys Asp Pro Asp Phe Leu Asp Ala Ala Leu Asp Phe 
    290                 295                 300                 


Asn Tyr Leu Asp Leu Ser Ala Leu Leu Arg Asn Ser Phe His Arg Tyr 
305                 310                 315                 320 


Ala Val Asp Val Leu Lys Ser Gly Arg Cys Gln Met Leu Asp Arg Arg 
                325                 330                 335     


Thr Val Glu Met Ala Phe Ala Tyr Ala Leu Ala Leu Phe Ala Ala Ala 
            340                 345                 350         


Arg Gln Glu Glu Ala Gly Ala Gln Val Ser Val Pro Arg Ala Leu Asp 
        355                 360                 365             


Arg Gln Ala Ala Leu Leu Gln Ile Gln Glu Phe Met Ile Thr Cys Leu 
    370                 375                 380                 


Ser Gln Thr Pro Pro Arg Thr Thr Leu Leu Leu Tyr Pro Thr Ala Val 
385                 390                 395                 400 


Asp Leu Ala Lys Arg Ala Leu Trp Thr Pro Asn Gln Ile Thr Asp Ile 
                405                 410                 415     


Thr Ser Leu Val Arg Leu Val Tyr Ile Leu Ser Lys Gln Asn Gln Gln 
            420                 425                 430         


His Leu Ile Pro Gln Trp Ala Leu Arg Gln Ile Ala Asp Phe Ala Leu 
        435                 440                 445             


Lys Leu His Lys Thr His Leu Ala Ser Phe Leu Ser Ala Phe Ala Arg 
    450                 455                 460                 


Gln Glu Leu Tyr Leu Met Gly Ser Leu Val His Ser Met Leu Val His 
465                 470                 475                 480 


Thr Thr Glu Arg Arg Glu Ile Phe Ile Val Glu Thr Gly Leu Cys Ser 
                485                 490                 495     


Leu Ala Glu Leu Ser His Phe Thr Gln Leu Leu Ala His Pro His His 
            500                 505                 510         


Glu Tyr Leu Ser Asp Leu Tyr Thr Pro Cys Ser Ser Ser Gly Arg Arg 
        515                 520                 525             


Asp His Ser Leu Glu Arg Leu Thr Arg Leu Phe Pro Asp Ala Thr Val 
    530                 535                 540                 


Pro Ala Thr Val Pro Ala Ala Leu Ser Ile Leu Ser Thr Met Gln Pro 
545                 550                 555                 560 


Ser Thr Leu Glu Thr Phe Pro Asp Leu Phe Cys Leu Pro Leu Gly Glu 
                565                 570                 575     


Ser Phe Ser Ala Leu Thr Val Ser Glu His Val Ser Tyr Ile Val Thr 
            580                 585                 590         


Asn Gln Tyr Leu Ile Lys Gly Ile Ser Tyr Pro Val Ser Thr Thr Val 
        595                 600                 605             


Val Gly Gln Ser Leu Ile Ile Thr Gln Thr Asp Ser Gln Thr Lys Cys 
    610                 615                 620                 


Glu Leu Thr Arg Asn Met His Thr Thr His Ser Ile Thr Val Ala Leu 
625                 630                 635                 640 


Asn Ile Ser Leu Glu Asn Cys Ala Phe Cys Gln Ser Ala Leu Leu Glu 
                645                 650                 655     


Tyr Asp Asp Thr Gln Gly Val Ile Asn Ile Met Tyr Met His Asp Ser 
            660                 665                 670         


Asp Asp Val Leu Phe Ala Leu Asp Pro Tyr Asn Glu Val Val Val Ser 
        675                 680                 685             


Ser Pro Arg Thr His Tyr Leu Met Leu Leu Lys Asn Gly Thr Val Leu 
    690                 695                 700                 


Glu Val Thr Asp Val Val Val Asp Ala Thr Asp Ser Arg Leu Leu Gly 
705                 710                 715                 720 


Ser Gly Gly Ser Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro 
                725                 730                 735     


Thr Lys His His His His His His 
            740                 


<210>  28
<211>  719
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gH with truncated transmembrane domain 


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (24)..(717)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (718)..(719)
<223>  Transmembrane domain (truncated)

<400>  28

Met Arg Pro Gly Leu Pro Ser Tyr Leu Ile Ile Leu Ala Val Cys Leu 
1               5                   10                  15      


Phe Ser His Leu Leu Ser Ser Arg Tyr Gly Ala Glu Ala Val Ser Glu 
            20                  25                  30          


Pro Leu Asp Lys Ala Phe His Leu Leu Leu Asn Thr Tyr Gly Arg Pro 
        35                  40                  45              


Ile Arg Phe Leu Arg Glu Asn Thr Thr Gln Cys Thr Tyr Asn Ser Ser 
    50                  55                  60                  


Leu Arg Asn Ser Thr Val Val Arg Glu Asn Ala Ile Ser Phe Asn Phe 
65                  70                  75                  80  


Phe Gln Ser Tyr Asn Gln Tyr Tyr Val Phe His Met Pro Arg Cys Leu 
                85                  90                  95      


Phe Ala Gly Pro Leu Ala Glu Gln Phe Leu Asn Gln Val Asp Leu Thr 
            100                 105                 110         


Glu Thr Leu Glu Arg Tyr Gln Gln Arg Leu Asn Thr Tyr Ala Leu Val 
        115                 120                 125             


Ser Lys Asp Leu Ala Ser Tyr Arg Ser Phe Ser Gln Gln Leu Lys Ala 
    130                 135                 140                 


Gln Asp Ser Leu Gly Glu Gln Pro Thr Thr Val Pro Pro Pro Ile Asp 
145                 150                 155                 160 


Leu Ser Ile Pro His Val Trp Met Pro Pro Gln Thr Thr Pro His Gly 
                165                 170                 175     


Trp Thr Glu Ser His Thr Thr Ser Gly Leu His Arg Pro His Phe Asn 
            180                 185                 190         


Gln Thr Cys Ile Leu Phe Asp Gly His Asp Leu Leu Phe Ser Thr Val 
        195                 200                 205             


Thr Pro Cys Leu His Gln Gly Phe Tyr Leu Ile Asp Glu Leu Arg Tyr 
    210                 215                 220                 


Val Lys Ile Thr Leu Thr Glu Asp Phe Phe Val Val Thr Val Ser Ile 
225                 230                 235                 240 


Asp Asp Asp Thr Pro Met Leu Leu Ile Phe Gly His Leu Pro Arg Val 
                245                 250                 255     


Leu Phe Lys Ala Pro Tyr Gln Arg Asp Asn Phe Ile Leu Arg Gln Thr 
            260                 265                 270         


Glu Lys His Glu Leu Leu Val Leu Val Lys Lys Asp Gln Leu Asn Arg 
        275                 280                 285             


His Ser Tyr Leu Lys Asp Pro Asp Phe Leu Asp Ala Ala Leu Asp Phe 
    290                 295                 300                 


Asn Tyr Leu Asp Leu Ser Ala Leu Leu Arg Asn Ser Phe His Arg Tyr 
305                 310                 315                 320 


Ala Val Asp Val Leu Lys Ser Gly Arg Cys Gln Met Leu Asp Arg Arg 
                325                 330                 335     


Thr Val Glu Met Ala Phe Ala Tyr Ala Leu Ala Leu Phe Ala Ala Ala 
            340                 345                 350         


Arg Gln Glu Glu Ala Gly Ala Gln Val Ser Val Pro Arg Ala Leu Asp 
        355                 360                 365             


Arg Gln Ala Ala Leu Leu Gln Ile Gln Glu Phe Met Ile Thr Cys Leu 
    370                 375                 380                 


Ser Gln Thr Pro Pro Arg Thr Thr Leu Leu Leu Tyr Pro Thr Ala Val 
385                 390                 395                 400 


Asp Leu Ala Lys Arg Ala Leu Trp Thr Pro Asn Gln Ile Thr Asp Ile 
                405                 410                 415     


Thr Ser Leu Val Arg Leu Val Tyr Ile Leu Ser Lys Gln Asn Gln Gln 
            420                 425                 430         


His Leu Ile Pro Gln Trp Ala Leu Arg Gln Ile Ala Asp Phe Ala Leu 
        435                 440                 445             


Lys Leu His Lys Thr His Leu Ala Ser Phe Leu Ser Ala Phe Ala Arg 
    450                 455                 460                 


Gln Glu Leu Tyr Leu Met Gly Ser Leu Val His Ser Met Leu Val His 
465                 470                 475                 480 


Thr Thr Glu Arg Arg Glu Ile Phe Ile Val Glu Thr Gly Leu Cys Ser 
                485                 490                 495     


Leu Ala Glu Leu Ser His Phe Thr Gln Leu Leu Ala His Pro His His 
            500                 505                 510         


Glu Tyr Leu Ser Asp Leu Tyr Thr Pro Cys Ser Ser Ser Gly Arg Arg 
        515                 520                 525             


Asp His Ser Leu Glu Arg Leu Thr Arg Leu Phe Pro Asp Ala Thr Val 
    530                 535                 540                 


Pro Ala Thr Val Pro Ala Ala Leu Ser Ile Leu Ser Thr Met Gln Pro 
545                 550                 555                 560 


Ser Thr Leu Glu Thr Phe Pro Asp Leu Phe Cys Leu Pro Leu Gly Glu 
                565                 570                 575     


Ser Phe Ser Ala Leu Thr Val Ser Glu His Val Ser Tyr Ile Val Thr 
            580                 585                 590         


Asn Gln Tyr Leu Ile Lys Gly Ile Ser Tyr Pro Val Ser Thr Thr Val 
        595                 600                 605             


Val Gly Gln Ser Leu Ile Ile Thr Gln Thr Asp Ser Gln Thr Lys Cys 
    610                 615                 620                 


Glu Leu Thr Arg Asn Met His Thr Thr His Ser Ile Thr Val Ala Leu 
625                 630                 635                 640 


Asn Ile Ser Leu Glu Asn Cys Ala Phe Cys Gln Ser Ala Leu Leu Glu 
                645                 650                 655     


Tyr Asp Asp Thr Gln Gly Val Ile Asn Ile Met Tyr Met His Asp Ser 
            660                 665                 670         


Asp Asp Val Leu Phe Ala Leu Asp Pro Tyr Asn Glu Val Val Val Ser 
        675                 680                 685             


Ser Pro Arg Thr His Tyr Leu Met Leu Leu Lys Asn Gly Thr Val Leu 
    690                 695                 700                 


Glu Val Thr Asp Val Val Val Asp Ala Thr Asp Ser Arg Leu Leu 
705                 710                 715                 


<210>  29
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker from gH construct

<400>  29

Gly Ser Gly Gly Ser Gly 
1               5       


<210>  30
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spytag

<400>  30

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  31
<211>  278
<212>  PRT
<213>  Human cytomegalovirus


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (31)..(278)
<223>  Ectodomain

<400>  31

Met Cys Arg Arg Pro Asp Cys Gly Phe Ser Phe Ser Pro Gly Pro Val 
1               5                   10                  15      


Ile Leu Leu Trp Cys Cys Leu Leu Leu Pro Ile Val Ser Ser Ala Ala 
            20                  25                  30          


Val Ser Val Ala Pro Thr Ala Ala Glu Lys Val Pro Ala Glu Cys Pro 
        35                  40                  45              


Glu Leu Thr Arg Arg Cys Leu Leu Gly Glu Val Phe Glu Gly Asp Lys 
    50                  55                  60                  


Tyr Glu Ser Trp Leu Arg Pro Leu Val Asn Val Thr Gly Arg Asp Gly 
65                  70                  75                  80  


Pro Leu Ser Gln Leu Ile Arg Tyr Arg Pro Val Thr Pro Glu Ala Ala 
                85                  90                  95      


Asn Ser Val Leu Leu Asp Glu Ala Phe Leu Asp Thr Leu Ala Leu Leu 
            100                 105                 110         


Tyr Asn Asn Pro Asp Gln Leu Arg Ala Leu Leu Thr Leu Leu Ser Ser 
        115                 120                 125             


Asp Thr Ala Pro Arg Trp Met Thr Val Met Arg Gly Tyr Ser Glu Cys 
    130                 135                 140                 


Gly Asp Gly Ser Pro Ala Val Tyr Thr Cys Val Asp Asp Leu Cys Arg 
145                 150                 155                 160 


Gly Tyr Asp Leu Thr Arg Leu Ser Tyr Gly Arg Ser Ile Phe Thr Glu 
                165                 170                 175     


His Val Leu Gly Phe Glu Leu Val Pro Pro Ser Leu Phe Asn Val Val 
            180                 185                 190         


Val Ala Ile Arg Asn Glu Ala Thr Arg Thr Asn Arg Ala Val Arg Leu 
        195                 200                 205             


Pro Val Ser Thr Ala Ala Ala Pro Glu Gly Ile Thr Leu Phe Tyr Gly 
    210                 215                 220                 


Leu Tyr Asn Ala Val Lys Glu Phe Cys Leu Arg His Gln Leu Asp Pro 
225                 230                 235                 240 


Pro Leu Leu Arg His Leu Asp Lys Tyr Tyr Ala Gly Leu Pro Pro Glu 
                245                 250                 255     


Leu Lys Gln Thr Arg Val Asn Leu Pro Ala His Ser Arg Tyr Gly Pro 
            260                 265                 270         


Gln Ala Val Asp Ala Arg 
        275             


<210>  32
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UL130 - C-tag


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (26)..(214)
<223>  Ectodomain

<220>
<221>  misc_feature
<222>  (215)..(229)
<223>  Linker

<220>
<221>  misc_feature
<222>  (230)..(233)
<223>  C-tag

<400>  32

Met Leu Arg Leu Leu Leu Arg His His Phe His Cys Leu Leu Leu Cys 
1               5                   10                  15      


Ala Val Trp Ala Thr Pro Cys Leu Ala Ser Pro Trp Ser Thr Leu Thr 
            20                  25                  30          


Ala Asn Gln Asn Pro Ser Pro Pro Trp Ser Lys Leu Thr Tyr Ser Lys 
        35                  40                  45              


Pro His Asp Ala Ala Thr Phe Tyr Cys Pro Phe Leu Tyr Pro Ser Pro 
    50                  55                  60                  


Pro Arg Ser Pro Leu Gln Phe Ser Gly Phe Gln Arg Val Ser Thr Gly 
65                  70                  75                  80  


Pro Glu Cys Arg Asn Glu Thr Leu Tyr Leu Leu Tyr Asn Arg Glu Gly 
                85                  90                  95      


Gln Thr Leu Val Glu Arg Ser Ser Thr Trp Val Lys Lys Val Ile Trp 
            100                 105                 110         


Tyr Leu Ser Gly Arg Asn Gln Thr Ile Leu Gln Arg Met Pro Arg Thr 
        115                 120                 125             


Ala Ser Lys Pro Ser Asp Gly Asn Val Gln Ile Ser Val Glu Asp Ala 
    130                 135                 140                 


Lys Ile Phe Gly Ala His Met Val Pro Lys Gln Thr Lys Leu Leu Arg 
145                 150                 155                 160 


Phe Val Val Asn Asp Gly Thr Arg Tyr Gln Met Cys Val Met Lys Leu 
                165                 170                 175     


Glu Ser Trp Ala His Val Phe Arg Asp Tyr Ser Val Ser Phe Gln Val 
            180                 185                 190         


Arg Leu Thr Phe Thr Glu Ala Asn Asn Gln Thr Tyr Thr Phe Cys Thr 
        195                 200                 205             


His Pro Asn Leu Ile Val Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Gly Ser Glu Pro Glu Ala 
225                 230             


<210>  33
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UL130 (signal sequence and ectodomain)


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (26)..(214)
<223>  Ectodomain

<400>  33

Met Leu Arg Leu Leu Leu Arg His His Phe His Cys Leu Leu Leu Cys 
1               5                   10                  15      


Ala Val Trp Ala Thr Pro Cys Leu Ala Ser Pro Trp Ser Thr Leu Thr 
            20                  25                  30          


Ala Asn Gln Asn Pro Ser Pro Pro Trp Ser Lys Leu Thr Tyr Ser Lys 
        35                  40                  45              


Pro His Asp Ala Ala Thr Phe Tyr Cys Pro Phe Leu Tyr Pro Ser Pro 
    50                  55                  60                  


Pro Arg Ser Pro Leu Gln Phe Ser Gly Phe Gln Arg Val Ser Thr Gly 
65                  70                  75                  80  


Pro Glu Cys Arg Asn Glu Thr Leu Tyr Leu Leu Tyr Asn Arg Glu Gly 
                85                  90                  95      


Gln Thr Leu Val Glu Arg Ser Ser Thr Trp Val Lys Lys Val Ile Trp 
            100                 105                 110         


Tyr Leu Ser Gly Arg Asn Gln Thr Ile Leu Gln Arg Met Pro Arg Thr 
        115                 120                 125             


Ala Ser Lys Pro Ser Asp Gly Asn Val Gln Ile Ser Val Glu Asp Ala 
    130                 135                 140                 


Lys Ile Phe Gly Ala His Met Val Pro Lys Gln Thr Lys Leu Leu Arg 
145                 150                 155                 160 


Phe Val Val Asn Asp Gly Thr Arg Tyr Gln Met Cys Val Met Lys Leu 
                165                 170                 175     


Glu Ser Trp Ala His Val Phe Arg Asp Tyr Ser Val Ser Phe Gln Val 
            180                 185                 190         


Arg Leu Thr Phe Thr Glu Ala Asn Asn Gln Thr Tyr Thr Phe Cys Thr 
        195                 200                 205             


His Pro Asn Leu Ile Val 
    210                 


<210>  34
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker from UL130 construct

<400>  34

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  35
<211>  171
<212>  PRT
<213>  Human cytomegalovirus


<220>
<221>  misc_feature
<222>  (1)..(27)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (28)..(171)
<223>  Ectodomain

<400>  35

Met Ser Pro Lys Asp Leu Thr Pro Phe Leu Thr Ala Leu Trp Leu Leu 
1               5                   10                  15      


Leu Gly His Ser Arg Val Pro Arg Val Arg Ala Glu Glu Cys Cys Glu 
            20                  25                  30          


Phe Ile Asn Val Asn His Pro Pro Glu Arg Cys Tyr Asp Phe Lys Met 
        35                  40                  45              


Cys Asn Arg Phe Thr Val Ala Leu Arg Cys Pro Asp Gly Glu Val Cys 
    50                  55                  60                  


Tyr Ser Pro Glu Lys Thr Ala Glu Ile Arg Gly Ile Val Thr Thr Met 
65                  70                  75                  80  


Thr His Ser Leu Thr Arg Gln Val Val His Asn Lys Leu Thr Ser Cys 
                85                  90                  95      


Asn Tyr Asn Pro Leu Tyr Leu Glu Ala Asp Gly Arg Ile Arg Cys Gly 
            100                 105                 110         


Lys Val Asn Asp Lys Ala Gln Tyr Leu Leu Gly Ala Ala Gly Ser Val 
        115                 120                 125             


Pro Tyr Arg Trp Ile Asn Leu Glu Tyr Asp Lys Ile Thr Arg Ile Val 
    130                 135                 140                 


Gly Leu Asp Gln Tyr Leu Glu Ser Val Lys Lys His Lys Arg Leu Asp 
145                 150                 155                 160 


Val Cys Arg Ala Lys Met Gly Tyr Met Leu Gln 
                165                 170     


<210>  36
<211>  129
<212>  PRT
<213>  Human cytomegalovirus


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (19)..(129)
<223>  Ectodomain

<400>  36

Met Arg Leu Cys Arg Val Trp Leu Ser Val Cys Leu Cys Ala Val Val 
1               5                   10                  15      


Leu Gly Gln Cys Gln Arg Glu Thr Ala Glu Lys Asn Asp Tyr Tyr Arg 
            20                  25                  30          


Val Pro His Tyr Trp Asp Ala Cys Ser Arg Ala Leu Pro Asp Gln Thr 
        35                  40                  45              


Arg Tyr Lys Tyr Val Glu Gln Leu Val Asp Leu Thr Leu Asn Tyr His 
    50                  55                  60                  


Tyr Asp Ala Ser His Gly Leu Asp Asn Phe Asp Val Leu Lys Arg Ile 
65                  70                  75                  80  


Asn Val Thr Glu Val Ser Leu Leu Ile Ser Asp Phe Arg Arg Gln Asn 
                85                  90                  95      


Arg Arg Gly Gly Thr Asn Lys Arg Thr Thr Phe Asn Ala Ala Gly Ser 
            100                 105                 110         


Leu Ala Pro His Ala Arg Ser Leu Glu Phe Ser Val Arg Leu Phe Ala 
        115                 120                 125             


Asn 
    


<210>  37
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyCatcher-HBsAg


<220>
<221>  misc_feature
<222>  (1)..(92)
<223>  SpycatcherDeltaN1

<220>
<221>  misc_feature
<222>  (93)..(101)
<223>  Flexible Linker

<220>
<221>  misc_feature
<222>  (102)..(105)
<223>  PVTN linker

<220>
<221>  misc_feature
<222>  (106)..(331)
<223>  HBsAg

<220>
<221>  misc_feature
<222>  (332)..(335)
<223>  C-tag

<400>  37

Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
1               5                   10                  15      


Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
            20                  25                  30          


Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
        35                  40                  45              


Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
    50                  55                  60                  


Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
65                  70                  75                  80  


Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile Gly Ser Gly Gly 
                85                  90                  95      


Ser Gly Gly Ser Gly Pro Val Thr Asn Met Glu Asn Ile Thr Ser Gly 
            100                 105                 110         


Phe Leu Gly Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu Thr 
        115                 120                 125             


Arg Ile Leu Thr Ile Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser Leu 
    130                 135                 140                 


Asn Phe Leu Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln Ser 
145                 150                 155                 160 


Pro Thr Ser Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro Gly 
                165                 170                 175     


Tyr Arg Trp Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile Leu 
            180                 185                 190         


Leu Leu Cys Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly Met 
        195                 200                 205             


Leu Pro Val Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr Gly 
    210                 215                 220                 


Pro Cys Lys Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe Pro 
225                 230                 235                 240 


Ser Cys Cys Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile Pro 
                245                 250                 255     


Ile Pro Ser Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala Ser 
            260                 265                 270         


Val Arg Phe Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp Phe 
        275                 280                 285             


Val Gly Leu Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met Trp 
    290                 295                 300                 


Tyr Trp Gly Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro Leu 
305                 310                 315                 320 


Leu Pro Ile Phe Phe Cys Leu Trp Val Tyr Ile Glu Pro Glu Ala 
                325                 330                 335 


<210>  38
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpyCatcherDeltaN1 

<400>  38

Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
1               5                   10                  15      


Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
            20                  25                  30          


Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
        35                  40                  45              


Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
    50                  55                  60                  


Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
65                  70                  75                  80  


Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
                85                  90          


<210>  39
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flexible linker from SpyCatcher-HBsAg 

<400>  39

Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   


<210>  40
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PVTN linker from SpyCatcher-HBsAg 

<400>  40

Pro Val Thr Asn 
1               


<210>  41
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HBsAg

<400>  41

Met Glu Asn Ile Thr Ser Gly Phe Leu Gly Pro Leu Leu Val Leu Gln 
1               5                   10                  15      


Ala Gly Phe Phe Leu Leu Thr Arg Ile Leu Thr Ile Pro Gln Ser Leu 
            20                  25                  30          


Asp Ser Trp Trp Thr Ser Leu Asn Phe Leu Gly Gly Ser Pro Val Cys 
        35                  40                  45              


Leu Gly Gln Asn Ser Gln Ser Pro Thr Ser Asn His Ser Pro Thr Ser 
    50                  55                  60                  


Cys Pro Pro Ile Cys Pro Gly Tyr Arg Trp Met Cys Leu Arg Arg Phe 
65                  70                  75                  80  


Ile Ile Phe Leu Phe Ile Leu Leu Leu Cys Leu Ile Phe Leu Leu Val 
                85                  90                  95      


Leu Leu Asp Tyr Gln Gly Met Leu Pro Val Cys Pro Leu Ile Pro Gly 
            100                 105                 110         


Ser Thr Thr Thr Asn Thr Gly Pro Cys Lys Thr Cys Thr Thr Pro Ala 
        115                 120                 125             


Gln Gly Asn Ser Met Phe Pro Ser Cys Cys Cys Thr Lys Pro Thr Asp 
    130                 135                 140                 


Gly Asn Cys Thr Cys Ile Pro Ile Pro Ser Ser Trp Ala Phe Ala Lys 
145                 150                 155                 160 


Tyr Leu Trp Glu Trp Ala Ser Val Arg Phe Ser Trp Leu Ser Leu Leu 
                165                 170                 175     


Val Pro Phe Val Gln Trp Phe Val Gly Leu Ser Pro Thr Val Trp Leu 
            180                 185                 190         


Ser Ala Ile Trp Met Met Trp Tyr Trp Gly Pro Ser Leu Tyr Ser Ile 
        195                 200                 205             


Val Ser Pro Phe Ile Pro Leu Leu Pro Ile Phe Phe Cys Leu Trp Val 
    210                 215                 220                 


Tyr Ile 
225     


<210>  42
<211>  2235
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gH-(GSG)2-SpyTag-His (without introns) optimised for CHO expression


<220>
<221>  misc_feature
<222>  (1)..(69)
<223>  gH signal peptide

<220>
<221>  misc_feature
<222>  (70)..(2151)
<223>  gH ectodomain

<220>
<221>  misc_feature
<222>  (2152)..(2157)
<223>  gH truncated transmembrane domain

<220>
<221>  misc_feature
<222>  (2158)..(2175)
<223>  (GSG)2 linker

<220>
<221>  misc_feature
<222>  (2176)..(2214)
<223>  Spytag

<220>
<221>  misc_feature
<222>  (2215)..(2232)
<223>  Histag

<220>
<221>  misc_feature
<222>  (2233)..(2235)
<223>  Stop codon

<400>  42
atgaggcctg gcctgccttc ttatctgatc atcctggccg tgtgcctgtt ctcccatctg       60

ctgtcctcta gatacggcgc cgaggctgtg tctgagcctc tggataaggc ctttcatctg      120

ctgctgaaca cctacggcag acctatccgg ttcctgcgcg agaacaccac acagtgcacc      180

tacaactcca gcctgcggaa ctccacagtc gtgcgggaaa acgccatctc cttcaacttt      240

ttccagtcct acaaccagta ctatgtgttc cacatgcctc gctgcctgtt cgctggacct      300

ctggctgagc agttcctgaa ccaggtggac ctgaccgaga cactggaaag ataccagcag      360

cggctgaaca catatgccct ggtgtctaag gacctggcct cctacagatc cttcagccag      420

cagctgaagg ctcaggactc tctgggagag cagcctacaa cagtgcctcc tcctatcgac      480

ctgtctatcc ctcacgtgtg gatgcctcca cagaccacac ctcatggctg gaccgagtct      540

cataccacct ctggcctgca ccggcctcac ttcaaccaga cctgcatcct gttcgacggc      600

cacgacctgc tgttctccac cgtgacacca tgtctgcacc agggcttcta cctgatcgac      660

gagctgagat acgtgaagat taccctgaca gaggacttct tcgtggtcac cgtgtccatc      720

gacgacgaca cccctatgct gctgatcttc ggccatctgc ctcgggtgct gttcaaggcc      780

ccttaccagc gggacaactt catcctgaga cagaccgaga agcacgagct gctggtgctg      840

gtcaagaagg accagctgaa ccggcactcc tacctgaagg accctgactt cctggacgcc      900

gctctggact tcaactacct ggatctgagc gccctgctgc ggaacagctt tcacagatac      960

gccgtggacg tgctgaagtc tggcagatgc cagatgctgg acagacggac cgtggaaatg     1020

gccttcgctt acgccctggc tctgtttgcc gccgctagac aagaagaggc tggcgcccaa     1080

gtgtccgtgc ctagagcact ggatagacaa gccgctctgc tgcagatcca agagttcatg     1140

atcacatgcc tgtctcagac ccctcctcgg accacactgc tgctgtatcc taccgctgtg     1200

gatctggcca agagggctct gtggacccct aaccagatca ccgacatcac atccctcgtg     1260

cggctggtgt acatcctgtc caagcagaac cagcagcatc tgatccctca gtgggccctg     1320

aggcagatcg ctgattttgc cctgaagctg cacaagaccc acctggccag ctttctgtct     1380

gccttcgcca gacaagagct gtacctgatg ggcagcctgg tgcactctat gctggtgcat     1440

accaccgagc ggcgcgagat cttcatcgtg gaaaccggcc tgtgttccct ggccgagctg     1500

tctcacttta cccagctgct cgctcaccct caccacgagt acctgtccga cctgtacacc     1560

ccttgctcct ctagcggcag aagggaccac agcctggaaa gactgacccg gctgttccct     1620

gatgccaccg tgcctgctac agttcctgcc gctctgtcca tcctgagcac catgcagcct     1680

tccactctgg aaacattccc cgacctgttc tgcctgcctc tgggcgagtc tttttctgcc     1740

ctgaccgtgt ccgagcacgt gtcctacatc gtgaccaatc agtacctgat caagggcatc     1800

agctaccccg tgtccacaac cgtcgttggc cagagcctga tcatcaccca gaccgactct     1860

cagaccaagt gcgagctgac ccggaacatg cacacaaccc actccatcac cgtggctctg     1920

aacatctccc tggaaaactg cgccttctgc cagtctgccc tgctggaata cgatgacacc     1980

cagggcgtga tcaacatcat gtatatgcac gactccgacg acgtgctgtt tgccctggat     2040

ccttacaacg aggtggtggt gtctagcccc agaacacact acctgatgct gctcaagaac     2100

ggcaccgtgc tggaagtgac cgacgtggtg gtggacgcca ccgattctag attgctcggc     2160

tctggtggct ccggcgctca tatcgtgatg gtggatgctt acaagcccac caagcaccat     2220

catcaccacc actaa                                                      2235


<210>  43
<211>  837
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gL (without introns) optimised for CHO expression


<220>
<221>  misc_feature
<222>  (1)..(90)
<223>  gL signal peptide

<220>
<221>  misc_feature
<222>  (91)..(834)
<223>  gL ectodomain

<220>
<221>  misc_feature
<222>  (835)..(837)
<223>  Stop codon

<400>  43
atgtgcagaa ggcctgactg cggcttctcc ttctctcccg gacctgtgat cctgctgtgg       60

tgctgtctgc tgctgcccat cgtttcttcc gccgctgtgt ctgtggctcc taccgctgct      120

gaaaaggtgc cagctgagtg tcccgagctg accagaagat gtctgctggg cgaagtgttc      180

gagggcgata agtacgagtc ttggctgcgg cctctggtca acgtgaccgg aagagatgga      240

cccctgagcc agctgatccg gtacagacct gtgacacctg aggccgccaa ttccgtgctg      300

ctggatgagg ccttcctgga cacactggcc ctgctgtaca acaaccccga tcagctgaga      360

gccctgctga ccctgctgtc ctctgatacc gctcctagat ggatgaccgt gatgcggggc      420

tactctgagt gcggagatgg aagcccagcc gtgtacacct gtgtggacga tctgtgcaga      480

ggctacgacc tgaccagact gtcctacggc cggtccatct ttaccgagca tgtgctgggc      540

tttgagctgg tgcctcctag cctgttcaat gtggtggtgg ccatccggaa tgaggccacc      600

agaacaaata gagccgtgcg gctgcctgtg tctacagctg ctgctcctga gggcatcacc      660

ctgttctacg gcctgtacaa cgccgtgaaa gagttctgcc tgagacacca gctggaccct      720

ccactgctga ggcacctgga taagtactac gctggcctgc ctcctgagct gaagcagacc      780

agagtgaacc tgcctgctca ctccagatac ggccctcagg ctgtggacgc cagataa         837


<210>  44
<211>  516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UL128 (without introns) optimised for CHO expression


<220>
<221>  misc_feature
<222>  (1)..(81)
<223>  UL128 signal peptide

<220>
<221>  misc_feature
<222>  (82)..(513)
<223>  UL128 ectodomain

<220>
<221>  misc_feature
<222>  (514)..(516)
<223>  Stop codon

<400>  44
atgtccccta aggatctgac ccctttcctg accgctctgt ggctgctgct gggccattct       60

agagtgccta gagtcagagc cgaggaatgc tgcgagttca tcaacgtgaa ccatcctcca      120

gagcggtgct acgacttcaa gatgtgcaac agattcaccg tggctctgcg gtgccctgat      180

ggcgaagtgt gctactcccc tgaaaagacc gccgagatca gaggcatcgt gaccaccatg      240

acacactccc tgaccagaca ggtggtgcac aacaagctga ccagctgcaa ctacaaccct      300

ctgtacctgg aagccgacgg cagaatcaga tgcggcaaag tgaacgacaa ggcccagtac      360

ctgttgggcg ctgctggctc tgtgccctac agatggatca acctggaata cgacaagatc      420

acccggatcg tcggcctgga ccagtatctg gaatccgtga agaagcacaa gcggctggac      480

gtgtgcagag ccaagatggg ctatatgctg cagtaa                                516


<210>  45
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UL130-(G4S)3 - C-tag (without introns) optimised for CHO expression


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  UL130 signal peptide

<220>
<221>  misc_feature
<222>  (76)..(642)
<223>  UL130 ectodomain

<220>
<221>  misc_feature
<222>  (643)..(687)
<223>  (G4S)3 linker

<220>
<221>  misc_feature
<222>  (688)..(699)
<223>  C-tag

<220>
<221>  misc_feature
<222>  (700)..(702)
<223>  Stop codon

<400>  45
atgctgagac tgctgctgag acaccacttc cactgcctgc tgctgtgtgc cgtttgggct       60

acaccttgtc tggcctctcc atggtctacc ctgaccgcca accagaatcc ttctccacct      120

tggtccaagc tgacctactc caagcctcac gatgccgcta ccttctactg cccctttctg      180

tacccatctc cacctcggag ccctctgcag ttctctggct tccagagagt gtccaccgga      240

cctgagtgcc ggaacgagac actgtacctg ctgtacaacc gcgagggcca gacactggtg      300

gaaagatcct ctacctgggt caagaaagtg atctggtatc tgagcggccg gaaccagacc      360

atcctgcaga gaatgcctcg gaccgcctct aagccttctg acggcaacgt gcagatctcc      420

gtggaagatg ccaagatctt cggcgcccac atggtgccca agcagaccaa actgctgaga      480

ttcgtggtca acgacggcac ccgctaccag atgtgcgtga tgaagctgga aagctgggcc      540

cacgtgttcc gggattactc cgtgtctttc caagtgcggc tgaccttcac cgaggccaac      600

aaccagacct acaccttctg cacccatcct aacctgatcg tcggaggcgg aggatctggc      660

ggaggtggaa gtggcggagg cggatctgag cccgaggcct aa                         702


<210>  46
<211>  390
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UL131A (without introns) optimised for CHO expression


<220>
<221>  misc_feature
<222>  (1)..(54)
<223>  UL131 signal peptide

<220>
<221>  misc_feature
<222>  (55)..(387)
<223>  UL131 ectodomain

<220>
<221>  misc_feature
<222>  (388)..(390)
<223>  Stop codon

<400>  46
atgagactgt gcagagtgtg gctgtccgtg tgcctgtgtg ctgtggttct gggccagtgc       60

cagagagaga cagccgagaa gaacgactac tacagagtgc cccactactg ggacgcctgc      120

agtagagctt tgcccgatca gacccggtac aaatacgtgg aacagctggt ggatctgacc      180

ctgaactacc actacgacgc ctctcacggc ctggacaact tcgacgtgct gaagcggatc      240

aacgtgaccg aggtgtccct gctgatctct gacttccggc ggcagaatag aagaggcggc      300

accaacaagc ggaccacctt taatgctgcc ggctctctgg ctccccacgc cagatctctg      360

gaattttccg tgcggctgtt cgccaactaa                                       390


<210>  47
<211>  1587
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RSV-F-SpyTag-Ctag


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (1435)..(1515)
<223>  Foldon domain

<220>
<221>  misc_feature
<222>  (1516)..(1533)
<223>  (GSG)2 linker

<220>
<221>  misc_feature
<222>  (1534)..(1572)
<223>  Spytag

<220>
<221>  misc_feature
<222>  (1573)..(1584)
<223>  Ctag

<220>
<221>  misc_feature
<222>  (1585)..(1587)
<223>  Stop codon

<400>  47
atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc       60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg cagcgccgtg      120

agcaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag      180

ctgtccaaca tcaaagaaaa caagtgcaac ggcaccgacg ccaaagtgaa gctgatcaag      240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc      300

cccgccaccg gatctggcag cgccatttgc agcggcgtgg ccgtgtgtaa agtgctgcac      360

ctggaaggcg aagtgaacaa gatcaagtcc gccctgctgt ccaccaacaa ggccgtggtg      420

tccctgagca acggcgtgag cgtgctgacc ttcaaggtgc tggatctgaa gaactacatc      480

gacaagcagc tgctgcccat cctgaacaag cagagctgca gcatcagcaa catcgagaca      540

gtgatcgagt tccagcagaa gaacaaccgg ctgctggaaa tcacccggga gttcagcgtg      600

aacgccggag tgaccacccc cgtgtccacc tacatgctga ccaacagcga gctgctgtcc      660

ctgatcaatg acatgcccat caccaacgac cagaaaaagc tgatgagcaa caacgtgcag      720

atcgtgcggc agcagagcta ctccatcatg tgcatcatca aagaagaggt gctggcctac      780

gtggtgcagc tgcccctgta cggcgtgatc gacaccccct gctggaagct gcacaccagc      840

cccctgtgca caaccaacac caaagagggc agcaacatct gcctgacccg gaccgaccgg      900

ggctggtact gcgacaacgc cggcagcgtg tccttctttc cacaggccga gacatgcaag      960

gtgcagagca accgggtgtt ctgcgacacc atgaacagcc ggaccctgcc ctccgaagtg     1020

aacctgtgca acgtggacat cttcaacccc aagtacgact gcaagatcat gacctccaag     1080

accgacgtgt ccagctccgt gatcacctcc ctgggcgcca tcgtgtcctg ctacggcaag     1140

accaagtgca ccgccagcaa caagaacaga ggcatcatca agaccttcag caacggctgc     1200

gactacgtgt ccaataaggg cgtggacacc gtgtccgtgg gcaacacact gtactgcgtg     1260

aataagcagg aaggcaagag cctgtacgtg aagggcgagc ccatcatcaa cttctacgac     1320

cccctggtgt tccccagcga cgagttcgac gctagcatca gccaggtgaa cgagaagatc     1380

aaccagagcc tggccttcat cagaaagagc gacgaactgc tgtccgccat cggcggctac     1440

atccccgagg cccccagaga tggccaggcc tacgtgcgga aggacggcga gtgggtgctg     1500

ctgtctacat ttctgggaag cggaggctct ggtgcccata tcgtgatggt ggacgcctac     1560

aagcctacca aagagcccga ggcctaa                                         1587


<210>  48
<211>  1515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sc9-10 DS-Cav1 A149C Y458C (RSV-F)


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (1435)..(1515)
<223>  Foldon domain

<400>  48
atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc       60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg cagcgccgtg      120

agcaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag      180

ctgtccaaca tcaaagaaaa caagtgcaac ggcaccgacg ccaaagtgaa gctgatcaag      240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc      300

cccgccaccg gatctggcag cgccatttgc agcggcgtgg ccgtgtgtaa agtgctgcac      360

ctggaaggcg aagtgaacaa gatcaagtcc gccctgctgt ccaccaacaa ggccgtggtg      420

tccctgagca acggcgtgag cgtgctgacc ttcaaggtgc tggatctgaa gaactacatc      480

gacaagcagc tgctgcccat cctgaacaag cagagctgca gcatcagcaa catcgagaca      540

gtgatcgagt tccagcagaa gaacaaccgg ctgctggaaa tcacccggga gttcagcgtg      600

aacgccggag tgaccacccc cgtgtccacc tacatgctga ccaacagcga gctgctgtcc      660

ctgatcaatg acatgcccat caccaacgac cagaaaaagc tgatgagcaa caacgtgcag      720

atcgtgcggc agcagagcta ctccatcatg tgcatcatca aagaagaggt gctggcctac      780

gtggtgcagc tgcccctgta cggcgtgatc gacaccccct gctggaagct gcacaccagc      840

cccctgtgca caaccaacac caaagagggc agcaacatct gcctgacccg gaccgaccgg      900

ggctggtact gcgacaacgc cggcagcgtg tccttctttc cacaggccga gacatgcaag      960

gtgcagagca accgggtgtt ctgcgacacc atgaacagcc ggaccctgcc ctccgaagtg     1020

aacctgtgca acgtggacat cttcaacccc aagtacgact gcaagatcat gacctccaag     1080

accgacgtgt ccagctccgt gatcacctcc ctgggcgcca tcgtgtcctg ctacggcaag     1140

accaagtgca ccgccagcaa caagaacaga ggcatcatca agaccttcag caacggctgc     1200

gactacgtgt ccaataaggg cgtggacacc gtgtccgtgg gcaacacact gtactgcgtg     1260

aataagcagg aaggcaagag cctgtacgtg aagggcgagc ccatcatcaa cttctacgac     1320

cccctggtgt tccccagcga cgagttcgac gctagcatca gccaggtgaa cgagaagatc     1380

aaccagagcc tggccttcat cagaaagagc gacgaactgc tgtccgccat cggcggctac     1440

atccccgagg cccccagaga tggccaggcc tacgtgcgga aggacggcga gtgggtgctg     1500

ctgtctacat ttctg                                                      1515



<210>  49
<211>  1440
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sc-9-10 DS-Cav1 A149C Y458C (RSV-F) without the signal peptide

<220>
<221>  misc_feature
<222>  (1360)..(1440)
<223>  Foldon domain

<400>  49
cagaacatca ccgaggaatt ctaccagagc acctgcagcg ccgtgagcaa gggctacctg       60

agcgccctgc ggaccggctg gtacaccagc gtgatcacca tcgagctgtc caacatcaaa      120

gaaaacaagt gcaacggcac cgacgccaaa gtgaagctga tcaagcagga actggacaag      180

tacaagaacg ccgtgaccga gctgcagctg ctgatgcaga gcacccccgc caccggatct      240

ggcagcgcca tttgcagcgg cgtggccgtg tgtaaagtgc tgcacctgga aggcgaagtg      300

aacaagatca agtccgccct gctgtccacc aacaaggccg tggtgtccct gagcaacggc      360

gtgagcgtgc tgaccttcaa ggtgctggat ctgaagaact acatcgacaa gcagctgctg      420

cccatcctga acaagcagag ctgcagcatc agcaacatcg agacagtgat cgagttccag      480

cagaagaaca accggctgct ggaaatcacc cgggagttca gcgtgaacgc cggagtgacc      540

acccccgtgt ccacctacat gctgaccaac agcgagctgc tgtccctgat caatgacatg      600

cccatcacca acgaccagaa aaagctgatg agcaacaacg tgcagatcgt gcggcagcag      660

agctactcca tcatgtgcat catcaaagaa gaggtgctgg cctacgtggt gcagctgccc      720

ctgtacggcg tgatcgacac cccctgctgg aagctgcaca ccagccccct gtgcacaacc      780

aacaccaaag agggcagcaa catctgcctg acccggaccg accggggctg gtactgcgac      840

aacgccggca gcgtgtcctt ctttccacag gccgagacat gcaaggtgca gagcaaccgg      900

gtgttctgcg acaccatgaa cagccggacc ctgccctccg aagtgaacct gtgcaacgtg      960

gacatcttca accccaagta cgactgcaag atcatgacct ccaagaccga cgtgtccagc     1020

tccgtgatca cctccctggg cgccatcgtg tcctgctacg gcaagaccaa gtgcaccgcc     1080

agcaacaaga acagaggcat catcaagacc ttcagcaacg gctgcgacta cgtgtccaat     1140

aagggcgtgg acaccgtgtc cgtgggcaac acactgtact gcgtgaataa gcaggaaggc     1200

aagagcctgt acgtgaaggg cgagcccatc atcaacttct acgaccccct ggtgttcccc     1260

agcgacgagt tcgacgctag catcagccag gtgaacgaga agatcaacca gagcctggcc     1320

ttcatcagaa agagcgacga actgctgtcc gccatcggcg gctacatccc cgaggccccc     1380

agagatggcc aggcctacgt gcggaaggac ggcgagtggg tgctgctgtc tacatttctg     1440


<210>  50
<211>  528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RSV-F-SpyTag-Ctag


<220>
<221>  MISC_FEATURE
<222>  (1)..(25)
<223>  Signal peptide

<220>
<221>  MISC_FEATURE
<222>  (479)..(505)
<223>  Foldon domain

<220>
<221>  MISC_FEATURE
<222>  (506)..(511)
<223>  (GSG)2 linker

<220>
<221>  MISC_FEATURE
<222>  (512)..(524)
<223>  Spytag

<220>
<221>  MISC_FEATURE
<222>  (525)..(527)
<223>  Ctag

<400>  50

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Gly Ser Gly Ser Ala Ile Cys Ser Gly 
            100                 105                 110         


Val Ala Val Cys Lys Val Leu His Leu Glu Gly Glu Val Asn Lys Ile 
        115                 120                 125             


Lys Ser Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser Leu Ser Asn 
    130                 135                 140                 


Gly Val Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys Asn Tyr Ile 
145                 150                 155                 160 


Asp Lys Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys Ser Ile Ser 
                165                 170                 175     


Asn Ile Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu 
            180                 185                 190         


Glu Ile Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr Thr Pro Val 
        195                 200                 205             


Ser Thr Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp 
    210                 215                 220                 


Met Pro Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn Asn Val Gln 
225                 230                 235                 240 


Ile Val Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile Lys Glu Glu 
                245                 250                 255     


Val Leu Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr 
            260                 265                 270         


Pro Cys Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys 
        275                 280                 285             


Glu Gly Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys 
    290                 295                 300                 


Asp Asn Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu Thr Cys Lys 
305                 310                 315                 320 


Val Gln Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser Arg Thr Leu 
                325                 330                 335     


Pro Ser Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn Pro Lys Tyr 
            340                 345                 350         


Asp Cys Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser Ser Val Ile 
        355                 360                 365             


Thr Ser Leu Gly Ala Ile Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr 
    370                 375                 380                 


Ala Ser Asn Lys Asn Arg Gly Ile Ile Lys Thr Phe Ser Asn Gly Cys 
385                 390                 395                 400 


Asp Tyr Val Ser Asn Lys Gly Val Asp Thr Val Ser Val Gly Asn Thr 
                405                 410                 415     


Leu Tyr Cys Val Asn Lys Gln Glu Gly Lys Ser Leu Tyr Val Lys Gly 
            420                 425                 430         


Glu Pro Ile Ile Asn Phe Tyr Asp Pro Leu Val Phe Pro Ser Asp Glu 
        435                 440                 445             


Phe Asp Ala Ser Ile Ser Gln Val Asn Glu Lys Ile Asn Gln Ser Leu 
    450                 455                 460                 


Ala Phe Ile Arg Lys Ser Asp Glu Leu Leu Ser Ala Ile Gly Gly Tyr 
465                 470                 475                 480 


Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly 
                485                 490                 495     


Glu Trp Val Leu Leu Ser Thr Phe Leu Gly Ser Gly Gly Ser Gly Ala 
            500                 505                 510         


His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Glu Pro Glu Ala 
        515                 520                 525             


<210>  51
<211>  1010
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc-9-10 DS-Cav1 A149C Y458C (RSV-F)


<220>
<221>  MISC_FEATURE
<222>  (1)..(25)
<223>  Signal peptide

<220>
<221>  MISC_FEATURE
<222>  (479)..(505)
<223>  Foldon domain

<400>  51

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Gly Ser Gly Ser Ala Ile Cys Ser Gly 
            100                 105                 110         


Val Ala Val Cys Lys Val Leu His Leu Glu Gly Glu Val Asn Lys Ile 
        115                 120                 125             


Lys Ser Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser Leu Ser Asn 
    130                 135                 140                 


Gly Val Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys Asn Tyr Ile 
145                 150                 155                 160 


Asp Lys Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys Ser Ile Ser 
                165                 170                 175     


Asn Ile Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu 
            180                 185                 190         


Glu Ile Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr Thr Pro Val 
        195                 200                 205             


Ser Thr Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp 
    210                 215                 220                 


Met Pro Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn Asn Val Gln 
225                 230                 235                 240 


Ile Val Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile Lys Glu Glu 
                245                 250                 255     


Val Leu Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr 
            260                 265                 270         


Pro Cys Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys 
        275                 280                 285             


Glu Gly Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys 
    290                 295                 300                 


Asp Asn Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu Thr Cys Lys 
305                 310                 315                 320 


Val Gln Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser Arg Thr Leu 
                325                 330                 335     


Pro Ser Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn Pro Lys Tyr 
            340                 345                 350         


Asp Cys Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser Ser Val Ile 
        355                 360                 365             


Thr Ser Leu Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr 
    370                 375                 380                 


Ile Leu Thr Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr 
385                 390                 395                 400 


Glu Glu Phe Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu 
                405                 410                 415     


Ser Ala Leu Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu 
            420                 425                 430         


Ser Asn Ile Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys 
        435                 440                 445             


Leu Ile Lys Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu 
    450                 455                 460                 


Gln Leu Leu Met Gln Ser Thr Pro Ala Thr Gly Ser Gly Ser Ala Ile 
465                 470                 475                 480 


Cys Ser Gly Val Ala Val Cys Lys Val Leu His Leu Glu Gly Glu Val 
                485                 490                 495     


Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser 
            500                 505                 510         


Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys 
        515                 520                 525             


Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys 
    530                 535                 540                 


Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn 
545                 550                 555                 560 


Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr 
                565                 570                 575     


Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu 
            580                 585                 590         


Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn 
        595                 600                 605             


Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile 
    610                 615                 620                 


Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val 
625                 630                 635                 640 


Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr 
                645                 650                 655     


Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly 
            660                 665                 670         


Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu 
        675                 680                 685             


Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser 
    690                 695                 700                 


Arg Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn 
705                 710                 715                 720 


Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser 
                725                 730                 735     


Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys Tyr Gly Lys Thr 
            740                 745                 750         


Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile Lys Thr Phe Ser 
        755                 760                 765             


Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp Thr Val Ser Val 
    770                 775                 780                 


Gly Asn Thr Leu Tyr Cys Val Asn Lys Gln Glu Gly Lys Ser Leu Tyr 
785                 790                 795                 800 


Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro Leu Val Phe Pro 
                805                 810                 815     


Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn Glu Lys Ile Asn 
            820                 825                 830         


Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu Leu Ser Ala Ile 
        835                 840                 845             


Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg 
    850                 855                 860                 


Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Gly Ala Ile Val 
865                 870                 875                 880 


Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly 
                885                 890                 895     


Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly 
            900                 905                 910         


Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr Cys Val Asn Lys Gln 
        915                 920                 925             


Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr 
    930                 935                 940                 


Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln 
945                 950                 955                 960 


Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp 
                965                 970                 975     


Glu Leu Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp 
            980                 985                 990         


Gly Gln Ala Tyr Val Arg Lys Asp  Gly Glu Trp Val Leu  Leu Ser Thr 
        995                 1000                 1005             


Phe Leu  
    1010 



<210>  52
<211>  480
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sc9-10 DS-Cav1 A149C Y458C (RSV-F) without the signal peptide


<220>
<221>  MISC_FEATURE
<222>  (454)..(480)
<223>  Foldon domain

<400>  52

Gln Asn Ile Thr Glu Glu Phe Tyr Gln Ser Thr Cys Ser Ala Val Ser 
1               5                   10                  15      


Lys Gly Tyr Leu Ser Ala Leu Arg Thr Gly Trp Tyr Thr Ser Val Ile 
            20                  25                  30          


Thr Ile Glu Leu Ser Asn Ile Lys Glu Asn Lys Cys Asn Gly Thr Asp 
        35                  40                  45              


Ala Lys Val Lys Leu Ile Lys Gln Glu Leu Asp Lys Tyr Lys Asn Ala 
    50                  55                  60                  


Val Thr Glu Leu Gln Leu Leu Met Gln Ser Thr Pro Ala Thr Gly Ser 
65                  70                  75                  80  


Gly Ser Ala Ile Cys Ser Gly Val Ala Val Cys Lys Val Leu His Leu 
                85                  90                  95      


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
            100                 105                 110         


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val 
        115                 120                 125             


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn 
    130                 135                 140                 


Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 
145                 150                 155                 160 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
                165                 170                 175     


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
            180                 185                 190         


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
        195                 200                 205             


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
    210                 215                 220                 


Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
225                 230                 235                 240 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
                245                 250                 255     


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
            260                 265                 270         


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
        275                 280                 285             


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
    290                 295                 300                 


Thr Met Asn Ser Arg Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
305                 310                 315                 320 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
                325                 330                 335     


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
            340                 345                 350         


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
        355                 360                 365             


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
    370                 375                 380                 


Thr Val Ser Val Gly Asn Thr Leu Tyr Cys Val Asn Lys Gln Glu Gly 
385                 390                 395                 400 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
                405                 410                 415     


Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
            420                 425                 430         


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
        435                 440                 445             


Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
    450                 455                 460                 


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
465                 470                 475                 480 



<210>  53
<211>  1704
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RSV-F DS-Cav1-SpyTag-Ctag


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (1552)..(1632)
<223>  Foldon domain

<220>
<221>  misc_feature
<222>  (1633)..(1650)
<223>  (GSG)2 linker

<220>
<221>  misc_feature
<222>  (1651)..(1689)
<223>  Spytag

<220>
<221>  misc_feature
<222>  (1690)..(1701)
<223>  Ctag

<220>
<221>  misc_feature
<222>  (1702)..(1704)
<223>  Stop codon

<400>  53
atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc       60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg cagcgccgtg      120

agcaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag      180

ctgtccaaca tcaaagaaaa caagtgcaac ggcaccgacg ccaaagtgaa gctgatcaag      240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc      300

cccgccacca acaacagagc cagaagagag ctgccccggt tcatgaacta caccctgaac      360

aacgccaaga aaaccaacgt gaccctgagc aagaagagaa agagaagatt cctgggcttc      420

ctgctgggcg tgggcagcgc cattgccagc ggcgtggccg tgtgtaaagt gctgcacctg      480

gaaggcgaag tgaacaagat caagtccgcc ctgctgtcca ccaacaaggc cgtggtgtcc      540

ctgagcaacg gcgtgagcgt gctgaccttc aaggtgctgg atctgaagaa ctacatcgac      600

aagcagctgc tgcccatcct gaacaagcag agctgcagca tcagcaacat cgagacagtg      660

atcgagttcc agcagaagaa caaccggctg ctggaaatca cccgggagtt cagcgtgaac      720

gccggagtga ccacccccgt gtccacctac atgctgacca acagcgagct gctgtccctg      780

atcaatgaca tgcccatcac caacgaccag aaaaagctga tgagcaacaa cgtgcagatc      840

gtgcggcagc agagctactc catcatgtgc atcatcaaag aagaggtgct ggcctacgtg      900

gtgcagctgc ccctgtacgg cgtgatcgac accccctgct ggaagctgca caccagcccc      960

ctgtgcacaa ccaacaccaa agagggcagc aacatctgcc tgacccggac cgaccggggc     1020

tggtactgcg acaacgccgg cagcgtgtcc ttctttccac aggccgagac atgcaaggtg     1080

cagagcaacc gggtgttctg cgacaccatg aacagcctga ccctgccctc cgaagtgaac     1140

ctgtgcaacg tggacatctt caaccccaag tacgactgca agatcatgac ctccaagacc     1200

gacgtgtcca gctccgtgat cacctccctg ggcgccatcg tgtcctgcta cggcaagacc     1260

aagtgcaccg ccagcaacaa gaacagaggc atcatcaaga ccttcagcaa cggctgcgac     1320

tacgtgtcca ataagggcgt ggacaccgtg tccgtgggca acacactgta ctacgtgaat     1380

aagcaggaag gcaagagcct gtacgtgaag ggcgagccca tcatcaactt ctacgacccc     1440

ctggtgttcc ccagcgacga gttcgacgcc agcatcagcc aggtgaacga gaagatcaac     1500

cagagcctgg ccttcatcag aaagagcgac gaactgctgt ccgccatcgg cggctacatc     1560

cccgaggccc ccagagatgg ccaggcctac gtgcggaagg acggcgagtg ggtgctgctg     1620

tctacatttc tgggaagcgg aggctctggt gcccatatcg tgatggtgga cgcctacaag     1680

cctaccaaag agcccgaggc ctaa                                            1704


<210>  54
<211>  1632
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RSV-F DS-Cav1


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  Signal peptide

<220>
<221>  misc_feature
<222>  (1552)..(1632)
<223>  Foldon domain

<400>  54
atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc       60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg cagcgccgtg      120

agcaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag      180

ctgtccaaca tcaaagaaaa caagtgcaac ggcaccgacg ccaaagtgaa gctgatcaag      240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc      300

cccgccacca acaacagagc cagaagagag ctgccccggt tcatgaacta caccctgaac      360

aacgccaaga aaaccaacgt gaccctgagc aagaagagaa agagaagatt cctgggcttc      420

ctgctgggcg tgggcagcgc cattgccagc ggcgtggccg tgtgtaaagt gctgcacctg      480

gaaggcgaag tgaacaagat caagtccgcc ctgctgtcca ccaacaaggc cgtggtgtcc      540

ctgagcaacg gcgtgagcgt gctgaccttc aaggtgctgg atctgaagaa ctacatcgac      600

aagcagctgc tgcccatcct gaacaagcag agctgcagca tcagcaacat cgagacagtg      660

atcgagttcc agcagaagaa caaccggctg ctggaaatca cccgggagtt cagcgtgaac      720

gccggagtga ccacccccgt gtccacctac atgctgacca acagcgagct gctgtccctg      780

atcaatgaca tgcccatcac caacgaccag aaaaagctga tgagcaacaa cgtgcagatc      840

gtgcggcagc agagctactc catcatgtgc atcatcaaag aagaggtgct ggcctacgtg      900

gtgcagctgc ccctgtacgg cgtgatcgac accccctgct ggaagctgca caccagcccc      960

ctgtgcacaa ccaacaccaa agagggcagc aacatctgcc tgacccggac cgaccggggc     1020

tggtactgcg acaacgccgg cagcgtgtcc ttctttccac aggccgagac atgcaaggtg     1080

cagagcaacc gggtgttctg cgacaccatg aacagcctga ccctgccctc cgaagtgaac     1140

ctgtgcaacg tggacatctt caaccccaag tacgactgca agatcatgac ctccaagacc     1200

gacgtgtcca gctccgtgat cacctccctg ggcgccatcg tgtcctgcta cggcaagacc     1260

aagtgcaccg ccagcaacaa gaacagaggc atcatcaaga ccttcagcaa cggctgcgac     1320

tacgtgtcca ataagggcgt ggacaccgtg tccgtgggca acacactgta ctacgtgaat     1380

aagcaggaag gcaagagcct gtacgtgaag ggcgagccca tcatcaactt ctacgacccc     1440

ctggtgttcc ccagcgacga gttcgacgcc agcatcagcc aggtgaacga gaagatcaac     1500

cagagcctgg ccttcatcag aaagagcgac gaactgctgt ccgccatcgg cggctacatc     1560

cccgaggccc ccagagatgg ccaggcctac gtgcggaagg acggcgagtg ggtgctgctg     1620

tctacatttc tg                                                         1632


<210>  55
<211>  1557
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RSV-F DS-Cav1 without the signal peptide


<220>
<221>  misc_feature
<222>  (1477)..(1557)
<223>  Foldon domain

<400>  55
cagaacatca ccgaggaatt ctaccagagc acctgcagcg ccgtgagcaa gggctacctg       60

agcgccctgc ggaccggctg gtacaccagc gtgatcacca tcgagctgtc caacatcaaa      120

gaaaacaagt gcaacggcac cgacgccaaa gtgaagctga tcaagcagga actggacaag      180

tacaagaacg ccgtgaccga gctgcagctg ctgatgcaga gcacccccgc caccaacaac      240

agagccagaa gagagctgcc ccggttcatg aactacaccc tgaacaacgc caagaaaacc      300

aacgtgaccc tgagcaagaa gagaaagaga agattcctgg gcttcctgct gggcgtgggc      360

agcgccattg ccagcggcgt ggccgtgtgt aaagtgctgc acctggaagg cgaagtgaac      420

aagatcaagt ccgccctgct gtccaccaac aaggccgtgg tgtccctgag caacggcgtg      480

agcgtgctga ccttcaaggt gctggatctg aagaactaca tcgacaagca gctgctgccc      540

atcctgaaca agcagagctg cagcatcagc aacatcgaga cagtgatcga gttccagcag      600

aagaacaacc ggctgctgga aatcacccgg gagttcagcg tgaacgccgg agtgaccacc      660

cccgtgtcca cctacatgct gaccaacagc gagctgctgt ccctgatcaa tgacatgccc      720

atcaccaacg accagaaaaa gctgatgagc aacaacgtgc agatcgtgcg gcagcagagc      780

tactccatca tgtgcatcat caaagaagag gtgctggcct acgtggtgca gctgcccctg      840

tacggcgtga tcgacacccc ctgctggaag ctgcacacca gccccctgtg cacaaccaac      900

accaaagagg gcagcaacat ctgcctgacc cggaccgacc ggggctggta ctgcgacaac      960

gccggcagcg tgtccttctt tccacaggcc gagacatgca aggtgcagag caaccgggtg     1020

ttctgcgaca ccatgaacag cctgaccctg ccctccgaag tgaacctgtg caacgtggac     1080

atcttcaacc ccaagtacga ctgcaagatc atgacctcca agaccgacgt gtccagctcc     1140

gtgatcacct ccctgggcgc catcgtgtcc tgctacggca agaccaagtg caccgccagc     1200

aacaagaaca gaggcatcat caagaccttc agcaacggct gcgactacgt gtccaataag     1260

ggcgtggaca ccgtgtccgt gggcaacaca ctgtactacg tgaataagca ggaaggcaag     1320

agcctgtacg tgaagggcga gcccatcatc aacttctacg accccctggt gttccccagc     1380

gacgagttcg acgccagcat cagccaggtg aacgagaaga tcaaccagag cctggccttc     1440

atcagaaaga gcgacgaact gctgtccgcc atcggcggct acatccccga ggcccccaga     1500

gatggccagg cctacgtgcg gaaggacggc gagtgggtgc tgctgtctac atttctg        1557


<210>  56
<211>  567
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RSV-F DS-Cav1-SpyTag-Ctag


<220>
<221>  MISC_FEATURE
<222>  (1)..(25)
<223>  Signal peptide

<220>
<221>  MISC_FEATURE
<222>  (518)..(544)
<223>  Foldon domain

<220>
<221>  MISC_FEATURE
<222>  (545)..(550)
<223>  Linker

<220>
<221>  MISC_FEATURE
<222>  (551)..(563)
<223>  Spytag

<220>
<221>  MISC_FEATURE
<222>  (564)..(567)
<223>  Ctag

<400>  56

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 
            100                 105                 110         


Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 
        115                 120                 125             


Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 
    130                 135                 140                 


Gly Ser Ala Ile Ala Ser Gly Val Ala Val Cys Lys Val Leu His Leu 
145                 150                 155                 160 


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
                165                 170                 175     


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val 
            180                 185                 190         


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn 
        195                 200                 205             


Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 
    210                 215                 220                 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
225                 230                 235                 240 


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
                245                 250                 255     


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
            260                 265                 270         


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
        275                 280                 285             


Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
    290                 295                 300                 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
305                 310                 315                 320 


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
                325                 330                 335     


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
            340                 345                 350         


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
        355                 360                 365             


Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
    370                 375                 380                 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
385                 390                 395                 400 


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
                405                 410                 415     


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
            420                 425                 430         


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
        435                 440                 445             


Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 
    450                 455                 460                 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
465                 470                 475                 480 


Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
                485                 490                 495     


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
            500                 505                 510         


Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
        515                 520                 525             


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
    530                 535                 540                 


Gly Ser Gly Gly Ser Gly Ala His Ile Val Met Val Asp Ala Tyr Lys 
545                 550                 555                 560 


Pro Thr Lys Glu Pro Glu Ala 
                565         


<210>  57
<211>  544
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RSV-F DS-Cav1


<220>
<221>  MISC_FEATURE
<222>  (1)..(25)
<223>  Signal peptide

<220>
<221>  MISC_FEATURE
<222>  (518)..(544)
<223>  Foldon domain

<400>  57

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 
            100                 105                 110         


Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 
        115                 120                 125             


Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 
    130                 135                 140                 


Gly Ser Ala Ile Ala Ser Gly Val Ala Val Cys Lys Val Leu His Leu 
145                 150                 155                 160 


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
                165                 170                 175     


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Phe Lys Val 
            180                 185                 190         


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Leu Asn 
        195                 200                 205             


Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 
    210                 215                 220                 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
225                 230                 235                 240 


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
                245                 250                 255     


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
            260                 265                 270         


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
        275                 280                 285             


Met Cys Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
    290                 295                 300                 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
305                 310                 315                 320 


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
                325                 330                 335     


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
            340                 345                 350         


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
        355                 360                 365             


Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
    370                 375                 380                 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
385                 390                 395                 400 


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
                405                 410                 415     


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
            420                 425                 430         


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
        435                 440                 445             


Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 
    450                 455                 460                 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
465                 470                 475                 480 


Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
                485                 490                 495     


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
            500                 505                 510         


Leu Ser Ala Ile Gly Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln 
        515                 520                 525             


Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu 
    530                 535                 540                 


<210>  58
<211>  519
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RSV-F DS-Cav1 without the signal peptide


<220>
<221>  MISC_FEATURE
<222>  (493)..(519)
<223>  Foldon domain

<400>  58

Gln Asn Ile Thr Glu Glu Phe Tyr Gln Ser Thr Cys Ser Ala Val Ser 
1               5                   10                  15      


Lys Gly Tyr Leu Ser Ala Leu Arg Thr Gly Trp Tyr Thr Ser Val Ile 
            20                  25                  30          


Thr Ile Glu Leu Ser Asn Ile Lys Glu Asn Lys Cys Asn Gly Thr Asp 
        35                  40                  45              


Ala Lys Val Lys Leu Ile Lys Gln Glu Leu Asp Lys Tyr Lys Asn Ala 
    50                  55                  60                  


Val Thr Glu Leu Gln Leu Leu Met Gln Ser Thr Pro Ala Thr Asn Asn 
65                  70                  75                  80  


Arg Ala Arg Arg Glu Leu Pro Arg Phe Met Asn Tyr Thr Leu Asn Asn 
                85                  90                  95      


Ala Lys Lys Thr Asn Val Thr Leu Ser Lys Lys Arg Lys Arg Arg Phe 
            100                 105                 110         


Leu Gly Phe Leu Leu Gly Val Gly Ser Ala Ile Ala Ser Gly Val Ala 
        115                 120                 125             


Val Cys Lys Val Leu His Leu Glu Gly Glu Val Asn Lys Ile Lys Ser 
    130                 135                 140                 


Ala Leu Leu Ser Thr Asn Lys Ala Val Val Ser Leu Ser Asn Gly Val 
145                 150                 155                 160 


Ser Val Leu Thr Phe Lys Val Leu Asp Leu Lys Asn Tyr Ile Asp Lys 
                165                 170                 175     


Gln Leu Leu Pro Ile Leu Asn Lys Gln Ser Cys Ser Ile Ser Asn Ile 
            180                 185                 190         


Glu Thr Val Ile Glu Phe Gln Gln Lys Asn Asn Arg Leu Leu Glu Ile 
        195                 200                 205             


Thr Arg Glu Phe Ser Val Asn Ala Gly Val Thr Thr Pro Val Ser Thr 
    210                 215                 220                 


Tyr Met Leu Thr Asn Ser Glu Leu Leu Ser Leu Ile Asn Asp Met Pro 
225                 230                 235                 240 


Ile Thr Asn Asp Gln Lys Lys Leu Met Ser Asn Asn Val Gln Ile Val 
                245                 250                 255     


Arg Gln Gln Ser Tyr Ser Ile Met Cys Ile Ile Lys Glu Glu Val Leu 
            260                 265                 270         


Ala Tyr Val Val Gln Leu Pro Leu Tyr Gly Val Ile Asp Thr Pro Cys 
        275                 280                 285             


Trp Lys Leu His Thr Ser Pro Leu Cys Thr Thr Asn Thr Lys Glu Gly 
    290                 295                 300                 


Ser Asn Ile Cys Leu Thr Arg Thr Asp Arg Gly Trp Tyr Cys Asp Asn 
305                 310                 315                 320 


Ala Gly Ser Val Ser Phe Phe Pro Gln Ala Glu Thr Cys Lys Val Gln 
                325                 330                 335     


Ser Asn Arg Val Phe Cys Asp Thr Met Asn Ser Leu Thr Leu Pro Ser 
            340                 345                 350         


Glu Val Asn Leu Cys Asn Val Asp Ile Phe Asn Pro Lys Tyr Asp Cys 
        355                 360                 365             


Lys Ile Met Thr Ser Lys Thr Asp Val Ser Ser Ser Val Ile Thr Ser 
    370                 375                 380                 


Leu Gly Ala Ile Val Ser Cys Tyr Gly Lys Thr Lys Cys Thr Ala Ser 
385                 390                 395                 400 


Asn Lys Asn Arg Gly Ile Ile Lys Thr Phe Ser Asn Gly Cys Asp Tyr 
                405                 410                 415     


Val Ser Asn Lys Gly Val Asp Thr Val Ser Val Gly Asn Thr Leu Tyr 
            420                 425                 430         


Tyr Val Asn Lys Gln Glu Gly Lys Ser Leu Tyr Val Lys Gly Glu Pro 
        435                 440                 445             


Ile Ile Asn Phe Tyr Asp Pro Leu Val Phe Pro Ser Asp Glu Phe Asp 
    450                 455                 460                 


Ala Ser Ile Ser Gln Val Asn Glu Lys Ile Asn Gln Ser Leu Ala Phe 
465                 470                 475                 480 


Ile Arg Lys Ser Asp Glu Leu Leu Ser Ala Ile Gly Gly Tyr Ile Pro 
                485                 490                 495     


Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp 
            500                 505                 510         


Val Leu Leu Ser Thr Phe Leu 
        515                 


<210>  59
<211>  2088
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gH without the signal peptide

<220>
<221>  misc_feature
<222>  (1077)..(1077)
<223>  C>A mutation at position 1077

<220>
<221>  misc_feature
<222>  (1)..(2082)
<223>  Ectodomain


<220>
<221>  misc_feature
<222>  (2083)..(2088)
<223>  Transmembrane domain (truncated)

<400>  59
cgatatggcg cagaagccgt atccgaaccg ctggacaaag cgtttcacct actgctcaac       60

acctacggga gacccatccg cttcctgcgt gaaaatacca cccagtgtac ctacaacagc      120

agcctccgta acagcacggt cgtcagggaa aacgccatca gtttcaactt tttccaaagc      180

tataatcaat actatgtatt ccatatgcct cgatgtcttt ttgcgggtcc tctggcggag      240

cagtttctga accaggtaga tctgaccgaa accctggaaa gataccaaca gagacttaac      300

acttacgcgc tggtatccaa agacctggcc agctaccgat ctttttcgca gcagctaaag      360

gcacaagaca gcctaggtga acagcccacc actgtgccac cgcccattga cctgtcaata      420

cctcacgttt ggatgccacc gcaaaccact ccacacggct ggacagaatc acataccacc      480

tcaggactac accgaccaca ctttaaccag acctgtatcc tctttgatgg acacgatcta      540

ctattcagca ccgtcacacc ttgtttgcac caaggctttt acctcatcga cgaactacgt      600

tacgttaaaa taacactgac cgaggacttc ttcgtagtta cggtgtccat agacgacgac      660

acacccatgc tgcttatctt cggccatctt ccacgcgtac ttttcaaagc gccctatcaa      720

cgcgacaact ttatactacg acaaactgaa aaacacgagc tcctggtgct agttaagaaa      780

gatcaactga accgtcactc ttatctcaaa gacccggact ttcttgacgc cgcacttgac      840

ttcaactacc tagacctcag cgcactacta cgtaacagct ttcaccgtta cgccgtggat      900

gtactcaaga gcggtcgatg tcagatgctg gaccgccgca cggtagaaat ggccttcgcc      960

tacgcattag cactgttcgc agcagcccga caagaagagg ccggcgccca agtctccgtc     1020

ccacgggccc tagaccgcca ggccgcactc ttacaaatac aagaatttat gatcacatgc     1080

ctctcacaaa caccaccacg caccacgttg ctgctgtatc ccacggccgt ggacctggcc     1140

aaacgagccc tttggacacc gaatcagatc accgacatca ccagcctcgt acgcctggtc     1200

tacatactct ctaaacagaa tcagcaacat ctcatccccc aatgggcact acgacagatc     1260

gccgactttg ccctaaaact acacaaaacg cacctggcct cttttctttc agccttcgca     1320

cgccaagaac tctacctcat gggcagcctc gtccactcca tgctggtaca tacgacggag     1380

agacgcgaaa tcttcatcgt agaaacgggc ctctgttcat tggccgagct atcacacttt     1440

acgcagttgt tagctcatcc acaccacgaa tacctcagcg acctgtacac accctgttcc     1500

agtagcgggc gacgcgatca ctcgctcgaa cgcctcacgc gtctcttccc cgatgccacc     1560

gtccccgcta ccgttcccgc cgccctctcc atcctatcta ccatgcaacc aagcacgctg     1620

gaaaccttcc ccgacctgtt ttgcttgccg ctcggcgaat ccttctccgc gctgaccgtc     1680

tccgaacacg tcagttatat cgtaacaaac cagtacctga tcaaaggtat ctcctaccct     1740

gtctccacca ccgtcgtagg ccagagcctc atcatcaccc agacggacag tcaaactaaa     1800

tgcgaactga cgcgcaacat gcataccaca cacagcatca cagtggcgct caacatttcg     1860

ctagaaaact gcgccttttg ccaaagcgcc ctgctagaat acgacgacac gcaaggcgtc     1920

atcaacatca tgtacatgca cgactcggac gacgtccttt tcgccctgga tccctacaac     1980

gaagtggtgg tctcatctcc gcgaactcac tacctcatgc ttttgaaaaa cggtacggta     2040

ctagaagtaa ctgacgtcgt cgtggacgcc accgacagtc gtctcctc                  2088


<210>  60
<211>  696
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gH without the signal peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(694)
<223>  Ectodomain

<220>
<221>  MISC_FEATURE
<222>  (694)..(696)
<223>  Transmembrane domain (truncated)

<400>  60

Arg Tyr Gly Ala Glu Ala Val Ser Glu Pro Leu Asp Lys Ala Phe His 
1               5                   10                  15      


Leu Leu Leu Asn Thr Tyr Gly Arg Pro Ile Arg Phe Leu Arg Glu Asn 
            20                  25                  30          


Thr Thr Gln Cys Thr Tyr Asn Ser Ser Leu Arg Asn Ser Thr Val Val 
        35                  40                  45              


Arg Glu Asn Ala Ile Ser Phe Asn Phe Phe Gln Ser Tyr Asn Gln Tyr 
    50                  55                  60                  


Tyr Val Phe His Met Pro Arg Cys Leu Phe Ala Gly Pro Leu Ala Glu 
65                  70                  75                  80  


Gln Phe Leu Asn Gln Val Asp Leu Thr Glu Thr Leu Glu Arg Tyr Gln 
                85                  90                  95      


Gln Arg Leu Asn Thr Tyr Ala Leu Val Ser Lys Asp Leu Ala Ser Tyr 
            100                 105                 110         


Arg Ser Phe Ser Gln Gln Leu Lys Ala Gln Asp Ser Leu Gly Glu Gln 
        115                 120                 125             


Pro Thr Thr Val Pro Pro Pro Ile Asp Leu Ser Ile Pro His Val Trp 
    130                 135                 140                 


Met Pro Pro Gln Thr Thr Pro His Gly Trp Thr Glu Ser His Thr Thr 
145                 150                 155                 160 


Ser Gly Leu His Arg Pro His Phe Asn Gln Thr Cys Ile Leu Phe Asp 
                165                 170                 175     


Gly His Asp Leu Leu Phe Ser Thr Val Thr Pro Cys Leu His Gln Gly 
            180                 185                 190         


Phe Tyr Leu Ile Asp Glu Leu Arg Tyr Val Lys Ile Thr Leu Thr Glu 
        195                 200                 205             


Asp Phe Phe Val Val Thr Val Ser Ile Asp Asp Asp Thr Pro Met Leu 
    210                 215                 220                 


Leu Ile Phe Gly His Leu Pro Arg Val Leu Phe Lys Ala Pro Tyr Gln 
225                 230                 235                 240 


Arg Asp Asn Phe Ile Leu Arg Gln Thr Glu Lys His Glu Leu Leu Val 
                245                 250                 255     


Leu Val Lys Lys Asp Gln Leu Asn Arg His Ser Tyr Leu Lys Asp Pro 
            260                 265                 270         


Asp Phe Leu Asp Ala Ala Leu Asp Phe Asn Tyr Leu Asp Leu Ser Ala 
        275                 280                 285             


Leu Leu Arg Asn Ser Phe His Arg Tyr Ala Val Asp Val Leu Lys Ser 
    290                 295                 300                 


Gly Arg Cys Gln Met Leu Asp Arg Arg Thr Val Glu Met Ala Phe Ala 
305                 310                 315                 320 


Tyr Ala Leu Ala Leu Phe Ala Ala Ala Arg Gln Glu Glu Ala Gly Ala 
                325                 330                 335     


Gln Val Ser Val Pro Arg Ala Leu Asp Arg Gln Ala Ala Leu Leu Gln 
            340                 345                 350         


Ile Gln Glu Phe Met Ile Thr Cys Leu Ser Gln Thr Pro Pro Arg Thr 
        355                 360                 365             


Thr Leu Leu Leu Tyr Pro Thr Ala Val Asp Leu Ala Lys Arg Ala Leu 
    370                 375                 380                 


Trp Thr Pro Asn Gln Ile Thr Asp Ile Thr Ser Leu Val Arg Leu Val 
385                 390                 395                 400 


Tyr Ile Leu Ser Lys Gln Asn Gln Gln His Leu Ile Pro Gln Trp Ala 
                405                 410                 415     


Leu Arg Gln Ile Ala Asp Phe Ala Leu Lys Leu His Lys Thr His Leu 
            420                 425                 430         


Ala Ser Phe Leu Ser Ala Phe Ala Arg Gln Glu Leu Tyr Leu Met Gly 
        435                 440                 445             


Ser Leu Val His Ser Met Leu Val His Thr Thr Glu Arg Arg Glu Ile 
    450                 455                 460                 


Phe Ile Val Glu Thr Gly Leu Cys Ser Leu Ala Glu Leu Ser His Phe 
465                 470                 475                 480 


Thr Gln Leu Leu Ala His Pro His His Glu Tyr Leu Ser Asp Leu Tyr 
                485                 490                 495     


Thr Pro Cys Ser Ser Ser Gly Arg Arg Asp His Ser Leu Glu Arg Leu 
            500                 505                 510         


Thr Arg Leu Phe Pro Asp Ala Thr Val Pro Ala Thr Val Pro Ala Ala 
        515                 520                 525             


Leu Ser Ile Leu Ser Thr Met Gln Pro Ser Thr Leu Glu Thr Phe Pro 
    530                 535                 540                 


Asp Leu Phe Cys Leu Pro Leu Gly Glu Ser Phe Ser Ala Leu Thr Val 
545                 550                 555                 560 


Ser Glu His Val Ser Tyr Ile Val Thr Asn Gln Tyr Leu Ile Lys Gly 
                565                 570                 575     


Ile Ser Tyr Pro Val Ser Thr Thr Val Val Gly Gln Ser Leu Ile Ile 
            580                 585                 590         


Thr Gln Thr Asp Ser Gln Thr Lys Cys Glu Leu Thr Arg Asn Met His 
        595                 600                 605             


Thr Thr His Ser Ile Thr Val Ala Leu Asn Ile Ser Leu Glu Asn Cys 
    610                 615                 620                 


Ala Phe Cys Gln Ser Ala Leu Leu Glu Tyr Asp Asp Thr Gln Gly Val 
625                 630                 635                 640 


Ile Asn Ile Met Tyr Met His Asp Ser Asp Asp Val Leu Phe Ala Leu 
                645                 650                 655     


Asp Pro Tyr Asn Glu Val Val Val Ser Ser Pro Arg Thr His Tyr Leu 
            660                 665                 670         


Met Leu Leu Lys Asn Gly Thr Val Leu Glu Val Thr Asp Val Val Val 
        675                 680                 685             


Asp Ala Thr Asp Ser Arg Leu Leu 
    690                 695     







