                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  STEVIOL GLYCOSIDE TRANSPORT

<130>  32869-WO-PCT

<150>  EP18170193.9
<151>  2018-04-30

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  4296
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  misc_feature
<223>  Polynucleotide sequence of the Open Reading Frame (ORF) belonging
       to the Yarrowia lipolytica transporter YALI0E25201

<400>  1
atgggtaaaa ccgaagtgac acaggagagt ctagaatgcg ggtcggtcac gtcctcgctg       60

gggaaaaagc ccttctccat catcacactc ttcaccggca gacgcattcc tccggtacct      120

actgaaaaac cagattcggc cgaagaacgg gccgggattc tgtcaaaatt gacctggcaa      180

tggcttagtc cattgttgaa agtgagtatg gctataggat aacttctgat gtcttcttca      240

atactaacac agactggtta cttacgaaac attgaacgtg aggatctgta taaagtgaga      300

gagagaaact cggcggctgt gatccagcag cgacttgaat ccaatctcga aaaacaatac      360

gccaagtacc acgccaaact gctcaagaaa ggactctcgg agcaagaggc gcatctcaag      420

ctgcaagatt cagccaaacc cctcgtcttg gctcttaacc agacgttttt ttggaagttc      480

tggctagccg gactgtttgc cctagtcaag gacctctgtg gaatcgcctc agctatggtg      540

tcacgtgttc tgatcgaata cattcaagac agatatctct acagggggac agaccgggaa      600

cctaaggtcg gccgaggagt cggcccctcg ataggcctat ttctactggc cgtaggagtc      660

actttcttct tcaaccacat gttctacaat gtcaagatgg ttggagctca ggctcgtgca      720

gctctggtgg ccgtcatcta cagcaagagt acccgtttga gcgccaaggg ccgagctcaa      780

tacaccacag gcaagatcac aaacttggca gctattgacg cacatcgagt tgatctcagt      840

tgtgaatctt tccactacat tactatcttt ttgcctgttg tgggttgtgc cattgctgta      900

ctcgtggtca acctcaaggt cgcagctcta gttggaattg cgaccatgat tgtcttgatc      960

tttgtcgtcg caggcatcac catcttctct atgaagctgc gagccatcat tgtcaagctc     1020

acggataagc gagtcacgta tatccgagaa gctctgcagt cgattagaat catcaagtac     1080

tacggctggg aggttcctta ctgtgacaag atcaagaagg tgcgtcttga cgagacccgt     1140

aactacgcca agatgggctc gattcgagga acagccattg gtatgtttca ggcactccct     1200

attttggcag gagcgttgtc tttcatcacc tacgctgctc taggtcatgg aactgatcct     1260

gctcgaatgt tctcttctct gacgcttttc aatttactcc tgcctgctct tgctgttctt     1320

ccccaggccc tccaggctgc tggagacgct cgagtggctc tcagacgtat ccagcggttc     1380

cttggggccg aggagtcgac tcccactaca gtttttgacg ctactcttga atctactgat     1440

gacgctgtga ttgtggaaga cgcctctttc atctggccag aagttgtcga tgataagagc     1500

gacaaagaga aggctaaaga tgcaaagaag gaggaaaagg ataagaagaa ggccgagaag     1560

aaggccaaga aggcggccaa gaaggcggcc aaggagatcg cggtggttgt ggaagaggag     1620

gtggaacacg aaaagaccga gggatccagt gagtctgaaa agggtactct taagtcgact     1680

ttcaagggct tcaacaacct gtctttcaaa atcaagcggg gtgaatttgt cgttgttacc     1740

ggtcccattg gttctggaaa gtcgtctctt cttgctgcca tcactggatc tatggttttg     1800

acaggcggtt ccgtgcgagt gtcgtccaca gagtggattg gatgtctgga gccgtggatt     1860

caaaacgcca cagttcgaga taacattgtg tttgggcgaa aattcgactc tgaatggtat     1920

agaactgtgg ttactgcctg tcagctgagc caggatctca aaataatgac tcacggagac     1980

aataccatga ttggagagcg aggcatcaca gtttcgggcg gtcaaaaagc tcgaatcaac     2040

ctcgcacgtg ctatatatgg aaaccccgag attctcatca tggacgacgt cctgtcggct     2100

gtggacgctc gagtaggtgc tggtattgtg gacgattgtc ttcgaggctt agccaagaac     2160

tccactcgaa ttctggccac ccatcagctg tctgtgctgc ctaaggctga tcatgtgatt     2220

ttcatggatg ccgaaggcca gtttcatatt ggtacgtacc aagagctgga ggctgacaat     2280

gagcagttca aggctctttt ggcggctggt tccatgtcca aggaggaggt ggttgctgtc     2340

gacgagactg aggttgttat tgaaggcgat cttgaagacg actgcgataa caaggaggag     2400

tatgaggatg cagctgagac catttccatt ttggcagatg ccactcaaga gctgcaaaag     2460

gtgaccacta cagtctcggc atttgaggag aacgataaca tgatggagga agaagagcga     2520

atgagagatg cagttggttt gcatgtgtac tggcagtatt ttcgtcaggc caaccccagt     2580

agggtcaagg taatgatgtt cattggcatg atcttcattt ccatgattgt gattgccttt     2640

ctgtttgtct tcacatctgt atggctctcg ttctggacag gtgaccgttt ccatgcctcc     2700

agaaacttct acaccggaat ttacatcatg ctgggtattc ttctgcttct tgctgtggca     2760

ggatacatga ttgtcaatga gatcaactct gccatggcag caagaaatct acacaatcat     2820

gctttggact cggtgttcgc tgcacgaact tctttcttcg ataccactcc tcagggtcgt     2880

atcatcaacc ggttcacccg agacacagac tctctggata acgagctggc tatgcgattg     2940

actatgttgt tctttggcgt ctccgcattc ttctccaact tcctgcttac ttgtgtctac     3000

gttccttatg tgactcttgt gcttgtccct gtcggttttg tcttctacgt ttctctaggt     3060

tactaccgaa agtcagctcg tgaagtcaag cgaattgact ccattgaacg gtcgcacatg     3120

atgagtgtct tcaacgagtc catttccggt atgcccgtca tcatcatgta caaggcccag     3180

catcggctca tgaacaagct tcaggctact ctcgatgata tggacagtgc ctacttcctc     3240

actgctgcaa accagcgatg gctgtctctc cgtctggatg gtctgggttc tttggtcgtt     3300

ctggtggcca ctattcttgt tgctgtcgga gtctttgatc tcaccccttc caacatgggt     3360

ctgatcattt ccgcggcctc ctttatcccc gaagtcatgt ctatggttgc ccaggccgtt     3420

gctgaactcg aaaactgcat gaacgccaca gagcgaattc tttactacaa ggacaacatt     3480

cctgctgagg ctgctcgaga agtggacggt acagagctcg accagcgacc caactggcct     3540

gagcagggag ccatcagctt caacaatgtg tccatgaagt accgagatgg acttccttac     3600

gtgctcaagt cattgtctgt cgactttcag ggaggacaca aggtgggtat ctgtggacga     3660

acaggagccg gtaagagtac catcttgcag actctgtatc gaattgtgga gcttgctgag     3720

ggttctatta ctattgatgg tgttgacatt tcgactattg gactgcatca gcttcggtct     3780

cagttgtcca tcattcccca ggagccagtt ttgttcctgg gcaccatccg gtctaatttg     3840

gatcctctgg agcaatactc tgatgctgag ctatggggtt ctctacgacg gtctggactt     3900

ctcgatgaag gagagactga gggtaagttt catctggatc aaaaggtgga ggctgacggc     3960

agcaacttct ctctaggtga gcgacagctg ctgactctag cccgagcact gcttagaaac     4020

accaaaattt tggtgctgga cgaagccaca tcaaatgtcg actacaagac ggacaagctg     4080

gttcaggaga ccatttcacg ggagtttggc cactgcacga ttctgtgtat cgcccatcga     4140

ctgcgaacca ttgccaagta tgatcgtatt ttggtgcttg agtccggcga gatcaaccag     4200

tacgacacgc cctggaactt gtacaacgac aaggagggta ttttccgagg tatgtgtgac     4260

acctccgggt tgaacgaggt agacttcaac aagtga                               4296


<210>  2
<211>  6595
<212>  DNA
<213>  Yarrowia lipolytica


<220>
<221>  misc_feature
<223>  Genomic sequence of the Yarrowia lipolytica transporter 
       YALI0E25201 comprising a 1000 bp flanking sequence upstream of 
       the ORF, the ORF and, downstream of the ORF, the terminator 
       sequence (300 bp) followed by a 999 bp flanking sequence

<400>  2
cacaaagtac aatacaagta catactgtac ctgtgagtca tagtgacatg agaaaccatc       60

gaaggggcac acactcactc acactgtgaa ttggctctgg tcgactgcct gatgctccta      120

taatcgatct acacaccaac tcctttctca tccgcatgga tatagtttca tatcagtgtg      180

catggtgcaa aacgccgttt gccacccaat agtgttctgt gcactaattt gacagcgaat      240

ttgccactgt ggggaacgtg gggaggaact ggaactacgg tactcgttcg aagttccgat      300

ccgaccggtg tgtgtcgagc aatggagtct gtgaaacctg tagtggtgct cgtggtggtg      360

gtggtggtgg ttgcacttgc agcatgtaag cagctgacgc gtcgcttaat agcacagtca      420

gttcgtatac tgtgatactt caatggtgtc aggaaggtga agaaacaacg ggtaataggg      480

catgttccat ttattttgaa cttgctgctt gaagtaggtt tggagactgc gttcggtgca      540

ttagcttctt gatatggagg cacggctctt cggggtccga ggtcgtggat agaagttgga      600

aagaggttct cggggttctt gtagatgaaa aaaacgacga agttgagata attaaacact      660

gtggagaaga gatatcgact gctgtgggtc acataacctc ttatatacgc gccaattctt      720

tcaccaaccc gcaagtcccc aaactcaatc cagaagacta tcaattttag acatgtatcg      780

gtcacatcaa tcgccgataa ccaaccagca agtggggaga cgccaccttt ttctcgcagc      840

ttttatactc ggcactgcgg cgttgctact taacctcctt gaatctcgat tggactgcta      900

gatcattgag atcagtcgac aaacagctca gatcagccac agatcagaca gctgatttca      960

gatcatcagt ttctcacacc acaccctcag tcttccacac atgggtaaaa ccgaagtgac     1020

acaggagagt ctagaatgcg ggtcggtcac gtcctcgctg gggaaaaagc ccttctccat     1080

catcacactc ttcaccggca gacgcattcc tccggtacct actgaaaaac cagattcggc     1140

cgaagaacgg gccgggattc tgtcaaaatt gacctggcaa tggcttagtc cattgttgaa     1200

agtgagtatg gctataggat aacttctgat gtcttcttca atactaacac agactggtta     1260

cttacgaaac attgaacgtg aggatctgta taaagtgaga gagagaaact cggcggctgt     1320

gatccagcag cgacttgaat ccaatctcga aaaacaatac gccaagtacc acgccaaact     1380

gctcaagaaa ggactctcgg agcaagaggc gcatctcaag ctgcaagatt cagccaaacc     1440

cctcgtcttg gctcttaacc agacgttttt ttggaagttc tggctagccg gactgtttgc     1500

cctagtcaag gacctctgtg gaatcgcctc agctatggtg tcacgtgttc tgatcgaata     1560

cattcaagac agatatctct acagggggac agaccgggaa cctaaggtcg gccgaggagt     1620

cggcccctcg ataggcctat ttctactggc cgtaggagtc actttcttct tcaaccacat     1680

gttctacaat gtcaagatgg ttggagctca ggctcgtgca gctctggtgg ccgtcatcta     1740

cagcaagagt acccgtttga gcgccaaggg ccgagctcaa tacaccacag gcaagatcac     1800

aaacttggca gctattgacg cacatcgagt tgatctcagt tgtgaatctt tccactacat     1860

tactatcttt ttgcctgttg tgggttgtgc cattgctgta ctcgtggtca acctcaaggt     1920

cgcagctcta gttggaattg cgaccatgat tgtcttgatc tttgtcgtcg caggcatcac     1980

catcttctct atgaagctgc gagccatcat tgtcaagctc acggataagc gagtcacgta     2040

tatccgagaa gctctgcagt cgattagaat catcaagtac tacggctggg aggttcctta     2100

ctgtgacaag atcaagaagg tgcgtcttga cgagacccgt aactacgcca agatgggctc     2160

gattcgagga acagccattg gtatgtttca ggcactccct attttggcag gagcgttgtc     2220

tttcatcacc tacgctgctc taggtcatgg aactgatcct gctcgaatgt tctcttctct     2280

gacgcttttc aatttactcc tgcctgctct tgctgttctt ccccaggccc tccaggctgc     2340

tggagacgct cgagtggctc tcagacgtat ccagcggttc cttggggccg aggagtcgac     2400

tcccactaca gtttttgacg ctactcttga atctactgat gacgctgtga ttgtggaaga     2460

cgcctctttc atctggccag aagttgtcga tgataagagc gacaaagaga aggctaaaga     2520

tgcaaagaag gaggaaaagg ataagaagaa ggccgagaag aaggccaaga aggcggccaa     2580

gaaggcggcc aaggagatcg cggtggttgt ggaagaggag gtggaacacg aaaagaccga     2640

gggatccagt gagtctgaaa agggtactct taagtcgact ttcaagggct tcaacaacct     2700

gtctttcaaa atcaagcggg gtgaatttgt cgttgttacc ggtcccattg gttctggaaa     2760

gtcgtctctt cttgctgcca tcactggatc tatggttttg acaggcggtt ccgtgcgagt     2820

gtcgtccaca gagtggattg gatgtctgga gccgtggatt caaaacgcca cagttcgaga     2880

taacattgtg tttgggcgaa aattcgactc tgaatggtat agaactgtgg ttactgcctg     2940

tcagctgagc caggatctca aaataatgac tcacggagac aataccatga ttggagagcg     3000

aggcatcaca gtttcgggcg gtcaaaaagc tcgaatcaac ctcgcacgtg ctatatatgg     3060

aaaccccgag attctcatca tggacgacgt cctgtcggct gtggacgctc gagtaggtgc     3120

tggtattgtg gacgattgtc ttcgaggctt agccaagaac tccactcgaa ttctggccac     3180

ccatcagctg tctgtgctgc ctaaggctga tcatgtgatt ttcatggatg ccgaaggcca     3240

gtttcatatt ggtacgtacc aagagctgga ggctgacaat gagcagttca aggctctttt     3300

ggcggctggt tccatgtcca aggaggaggt ggttgctgtc gacgagactg aggttgttat     3360

tgaaggcgat cttgaagacg actgcgataa caaggaggag tatgaggatg cagctgagac     3420

catttccatt ttggcagatg ccactcaaga gctgcaaaag gtgaccacta cagtctcggc     3480

atttgaggag aacgataaca tgatggagga agaagagcga atgagagatg cagttggttt     3540

gcatgtgtac tggcagtatt ttcgtcaggc caaccccagt agggtcaagg taatgatgtt     3600

cattggcatg atcttcattt ccatgattgt gattgccttt ctgtttgtct tcacatctgt     3660

atggctctcg ttctggacag gtgaccgttt ccatgcctcc agaaacttct acaccggaat     3720

ttacatcatg ctgggtattc ttctgcttct tgctgtggca ggatacatga ttgtcaatga     3780

gatcaactct gccatggcag caagaaatct acacaatcat gctttggact cggtgttcgc     3840

tgcacgaact tctttcttcg ataccactcc tcagggtcgt atcatcaacc ggttcacccg     3900

agacacagac tctctggata acgagctggc tatgcgattg actatgttgt tctttggcgt     3960

ctccgcattc ttctccaact tcctgcttac ttgtgtctac gttccttatg tgactcttgt     4020

gcttgtccct gtcggttttg tcttctacgt ttctctaggt tactaccgaa agtcagctcg     4080

tgaagtcaag cgaattgact ccattgaacg gtcgcacatg atgagtgtct tcaacgagtc     4140

catttccggt atgcccgtca tcatcatgta caaggcccag catcggctca tgaacaagct     4200

tcaggctact ctcgatgata tggacagtgc ctacttcctc actgctgcaa accagcgatg     4260

gctgtctctc cgtctggatg gtctgggttc tttggtcgtt ctggtggcca ctattcttgt     4320

tgctgtcgga gtctttgatc tcaccccttc caacatgggt ctgatcattt ccgcggcctc     4380

ctttatcccc gaagtcatgt ctatggttgc ccaggccgtt gctgaactcg aaaactgcat     4440

gaacgccaca gagcgaattc tttactacaa ggacaacatt cctgctgagg ctgctcgaga     4500

agtggacggt acagagctcg accagcgacc caactggcct gagcagggag ccatcagctt     4560

caacaatgtg tccatgaagt accgagatgg acttccttac gtgctcaagt cattgtctgt     4620

cgactttcag ggaggacaca aggtgggtat ctgtggacga acaggagccg gtaagagtac     4680

catcttgcag actctgtatc gaattgtgga gcttgctgag ggttctatta ctattgatgg     4740

tgttgacatt tcgactattg gactgcatca gcttcggtct cagttgtcca tcattcccca     4800

ggagccagtt ttgttcctgg gcaccatccg gtctaatttg gatcctctgg agcaatactc     4860

tgatgctgag ctatggggtt ctctacgacg gtctggactt ctcgatgaag gagagactga     4920

gggtaagttt catctggatc aaaaggtgga ggctgacggc agcaacttct ctctaggtga     4980

gcgacagctg ctgactctag cccgagcact gcttagaaac accaaaattt tggtgctgga     5040

cgaagccaca tcaaatgtcg actacaagac ggacaagctg gttcaggaga ccatttcacg     5100

ggagtttggc cactgcacga ttctgtgtat cgcccatcga ctgcgaacca ttgccaagta     5160

tgatcgtatt ttggtgcttg agtccggcga gatcaaccag tacgacacgc cctggaactt     5220

gtacaacgac aaggagggta ttttccgagg tatgtgtgac acctccgggt tgaacgaggt     5280

agacttcaac aagtgaaaaa ttacgatgag ggtagacagt ctgagatgag actgataaat     5340

tatagagaaa aaatgtttga ttatagcagt agtgaatgta ttgtatgtaa taagtggcca     5400

atcttggaat ggtggcctcc gtattaattc ttaccgaact cgtagtcacc tttgtaaagc     5460

gacagtgtct gttcacattg ggtcaaacat gtaaatattg tagctacgta gtctatagtc     5520

ctaagagaac gataccatag aaaatcgatt tccacagccg atcttttttg tcggtggcaa     5580

tcagtacttt tctttttctc aatattcgca ccgttcacaa ttacctcaaa ttgcttttcg     5640

gaagttgaga taggatcggg tcgggagtgt ttcggaagcg ggtcgggccg aaaaagccgt     5700

taccgggttt ccgaaactgc ctctgaaaag atgtggaatc acatggggga gtattagcag     5760

tgcctttgag atgtttcggt tgagctctgc ttagctttca ttaggcaaga gccgtgcttg     5820

atagatttct cgagcatgta gctcatacta gctcttgcac cattgaggtg aggtgaatga     5880

ggtgctgtat ccgtttttga tgacaccaat tgataataat tgtgagttat ggcaacgcaa     5940

agagtttgtt agaatgatag cagagaaatt ccatgcgaaa caccgctata tggcttgttt     6000

ggcagagtcc tacttcagct gtggtgttca tgccgaacta tagtatgtac gagaacaaag     6060

tagcccatta caatcacaag cggtcgtcga tactcaatca aaaataaacg tccagccaat     6120

gctacatcaa agacaccctt caaacagcat ctggacccgc agacttgcca ctatggatgc     6180

aggacgttaa ggggtggcat caagccgcag agagactata cgagtcttgg ctaactgtaa     6240

ttaggtgaca attgagagtg atgtccactg ataggctact tttacgagtg ataagtgaga     6300

gcgatagcct cggcgtctaa aaagggagca caatgagtgt cttattacga gtacggtgat     6360

atacattgtt gttcatctat agtttggatg tgtgtcaggt gcccgttttt atcagttcac     6420

cctacgagta catacagtat gtacttgtac agtacactta caagatctag tttgaccatg     6480

caactgaaca gaatacaact aaacagattt agttacacgc atacacacaa tagggtgtat     6540

aacctcagat atcagtccca tacttatagt actgtattgt tgtccttcac attct          6595


<210>  3
<211>  1414
<212>  PRT
<213>  Yarrowia lipolytica


<220>
<221>  MISC_FEATURE
<223>  Amino acid sequence of the Yarrowia lipolytica transporter 
       YALI0E25201

<400>  3

Met Gly Lys Thr Glu Val Thr Gln Glu Ser Leu Glu Cys Gly Ser Val 
1               5                   10                  15      


Thr Ser Ser Leu Gly Lys Lys Pro Phe Ser Ile Ile Thr Leu Phe Thr 
            20                  25                  30          


Gly Arg Arg Ile Pro Pro Val Pro Thr Glu Lys Pro Asp Ser Ala Glu 
        35                  40                  45              


Glu Arg Ala Gly Ile Leu Ser Lys Leu Thr Trp Gln Trp Leu Ser Pro 
    50                  55                  60                  


Leu Leu Lys Thr Gly Tyr Leu Arg Asn Ile Glu Arg Glu Asp Leu Tyr 
65                  70                  75                  80  


Lys Val Arg Glu Arg Asn Ser Ala Ala Val Ile Gln Gln Arg Leu Glu 
                85                  90                  95      


Ser Asn Leu Glu Lys Gln Tyr Ala Lys Tyr His Ala Lys Leu Leu Lys 
            100                 105                 110         


Lys Gly Leu Ser Glu Gln Glu Ala His Leu Lys Leu Gln Asp Ser Ala 
        115                 120                 125             


Lys Pro Leu Val Leu Ala Leu Asn Gln Thr Phe Phe Trp Lys Phe Trp 
    130                 135                 140                 


Leu Ala Gly Leu Phe Ala Leu Val Lys Asp Leu Cys Gly Ile Ala Ser 
145                 150                 155                 160 


Ala Met Val Ser Arg Val Leu Ile Glu Tyr Ile Gln Asp Arg Tyr Leu 
                165                 170                 175     


Tyr Arg Gly Thr Asp Arg Glu Pro Lys Val Gly Arg Gly Val Gly Pro 
            180                 185                 190         


Ser Ile Gly Leu Phe Leu Leu Ala Val Gly Val Thr Phe Phe Phe Asn 
        195                 200                 205             


His Met Phe Tyr Asn Val Lys Met Val Gly Ala Gln Ala Arg Ala Ala 
    210                 215                 220                 


Leu Val Ala Val Ile Tyr Ser Lys Ser Thr Arg Leu Ser Ala Lys Gly 
225                 230                 235                 240 


Arg Ala Gln Tyr Thr Thr Gly Lys Ile Thr Asn Leu Ala Ala Ile Asp 
                245                 250                 255     


Ala His Arg Val Asp Leu Ser Cys Glu Ser Phe His Tyr Ile Thr Ile 
            260                 265                 270         


Phe Leu Pro Val Val Gly Cys Ala Ile Ala Val Leu Val Val Asn Leu 
        275                 280                 285             


Lys Val Ala Ala Leu Val Gly Ile Ala Thr Met Ile Val Leu Ile Phe 
    290                 295                 300                 


Val Val Ala Gly Ile Thr Ile Phe Ser Met Lys Leu Arg Ala Ile Ile 
305                 310                 315                 320 


Val Lys Leu Thr Asp Lys Arg Val Thr Tyr Ile Arg Glu Ala Leu Gln 
                325                 330                 335     


Ser Ile Arg Ile Ile Lys Tyr Tyr Gly Trp Glu Val Pro Tyr Cys Asp 
            340                 345                 350         


Lys Ile Lys Lys Val Arg Leu Asp Glu Thr Arg Asn Tyr Ala Lys Met 
        355                 360                 365             


Gly Ser Ile Arg Gly Thr Ala Ile Gly Met Phe Gln Ala Leu Pro Ile 
    370                 375                 380                 


Leu Ala Gly Ala Leu Ser Phe Ile Thr Tyr Ala Ala Leu Gly His Gly 
385                 390                 395                 400 


Thr Asp Pro Ala Arg Met Phe Ser Ser Leu Thr Leu Phe Asn Leu Leu 
                405                 410                 415     


Leu Pro Ala Leu Ala Val Leu Pro Gln Ala Leu Gln Ala Ala Gly Asp 
            420                 425                 430         


Ala Arg Val Ala Leu Arg Arg Ile Gln Arg Phe Leu Gly Ala Glu Glu 
        435                 440                 445             


Ser Thr Pro Thr Thr Val Phe Asp Ala Thr Leu Glu Ser Thr Asp Asp 
    450                 455                 460                 


Ala Val Ile Val Glu Asp Ala Ser Phe Ile Trp Pro Glu Val Val Asp 
465                 470                 475                 480 


Asp Lys Ser Asp Lys Glu Lys Ala Lys Asp Ala Lys Lys Glu Glu Lys 
                485                 490                 495     


Asp Lys Lys Lys Ala Glu Lys Lys Ala Lys Lys Ala Ala Lys Lys Ala 
            500                 505                 510         


Ala Lys Glu Ile Ala Val Val Val Glu Glu Glu Val Glu His Glu Lys 
        515                 520                 525             


Thr Glu Gly Ser Ser Glu Ser Glu Lys Gly Thr Leu Lys Ser Thr Phe 
    530                 535                 540                 


Lys Gly Phe Asn Asn Leu Ser Phe Lys Ile Lys Arg Gly Glu Phe Val 
545                 550                 555                 560 


Val Val Thr Gly Pro Ile Gly Ser Gly Lys Ser Ser Leu Leu Ala Ala 
                565                 570                 575     


Ile Thr Gly Ser Met Val Leu Thr Gly Gly Ser Val Arg Val Ser Ser 
            580                 585                 590         


Thr Glu Trp Ile Gly Cys Leu Glu Pro Trp Ile Gln Asn Ala Thr Val 
        595                 600                 605             


Arg Asp Asn Ile Val Phe Gly Arg Lys Phe Asp Ser Glu Trp Tyr Arg 
    610                 615                 620                 


Thr Val Val Thr Ala Cys Gln Leu Ser Gln Asp Leu Lys Ile Met Thr 
625                 630                 635                 640 


His Gly Asp Asn Thr Met Ile Gly Glu Arg Gly Ile Thr Val Ser Gly 
                645                 650                 655     


Gly Gln Lys Ala Arg Ile Asn Leu Ala Arg Ala Ile Tyr Gly Asn Pro 
            660                 665                 670         


Glu Ile Leu Ile Met Asp Asp Val Leu Ser Ala Val Asp Ala Arg Val 
        675                 680                 685             


Gly Ala Gly Ile Val Asp Asp Cys Leu Arg Gly Leu Ala Lys Asn Ser 
    690                 695                 700                 


Thr Arg Ile Leu Ala Thr His Gln Leu Ser Val Leu Pro Lys Ala Asp 
705                 710                 715                 720 


His Val Ile Phe Met Asp Ala Glu Gly Gln Phe His Ile Gly Thr Tyr 
                725                 730                 735     


Gln Glu Leu Glu Ala Asp Asn Glu Gln Phe Lys Ala Leu Leu Ala Ala 
            740                 745                 750         


Gly Ser Met Ser Lys Glu Glu Val Val Ala Val Asp Glu Thr Glu Val 
        755                 760                 765             


Val Ile Glu Gly Asp Leu Glu Asp Asp Cys Asp Asn Lys Glu Glu Tyr 
    770                 775                 780                 


Glu Asp Ala Ala Glu Thr Ile Ser Ile Leu Ala Asp Ala Thr Gln Glu 
785                 790                 795                 800 


Leu Gln Lys Val Thr Thr Thr Val Ser Ala Phe Glu Glu Asn Asp Asn 
                805                 810                 815     


Met Met Glu Glu Glu Glu Arg Met Arg Asp Ala Val Gly Leu His Val 
            820                 825                 830         


Tyr Trp Gln Tyr Phe Arg Gln Ala Asn Pro Ser Arg Val Lys Val Met 
        835                 840                 845             


Met Phe Ile Gly Met Ile Phe Ile Ser Met Ile Val Ile Ala Phe Leu 
    850                 855                 860                 


Phe Val Phe Thr Ser Val Trp Leu Ser Phe Trp Thr Gly Asp Arg Phe 
865                 870                 875                 880 


His Ala Ser Arg Asn Phe Tyr Thr Gly Ile Tyr Ile Met Leu Gly Ile 
                885                 890                 895     


Leu Leu Leu Leu Ala Val Ala Gly Tyr Met Ile Val Asn Glu Ile Asn 
            900                 905                 910         


Ser Ala Met Ala Ala Arg Asn Leu His Asn His Ala Leu Asp Ser Val 
        915                 920                 925             


Phe Ala Ala Arg Thr Ser Phe Phe Asp Thr Thr Pro Gln Gly Arg Ile 
    930                 935                 940                 


Ile Asn Arg Phe Thr Arg Asp Thr Asp Ser Leu Asp Asn Glu Leu Ala 
945                 950                 955                 960 


Met Arg Leu Thr Met Leu Phe Phe Gly Val Ser Ala Phe Phe Ser Asn 
                965                 970                 975     


Phe Leu Leu Thr Cys Val Tyr Val Pro Tyr Val Thr Leu Val Leu Val 
            980                 985                 990         


Pro Val Gly Phe Val Phe Tyr Val  Ser Leu Gly Tyr Tyr  Arg Lys Ser 
        995                 1000                 1005             


Ala Arg  Glu Val Lys Arg Ile  Asp Ser Ile Glu Arg  Ser His Met 
    1010                 1015                 1020             


Met Ser  Val Phe Asn Glu Ser  Ile Ser Gly Met Pro  Val Ile Ile 
    1025                 1030                 1035             


Met Tyr  Lys Ala Gln His Arg  Leu Met Asn Lys Leu  Gln Ala Thr 
    1040                 1045                 1050             


Leu Asp  Asp Met Asp Ser Ala  Tyr Phe Leu Thr Ala  Ala Asn Gln 
    1055                 1060                 1065             


Arg Trp  Leu Ser Leu Arg Leu  Asp Gly Leu Gly Ser  Leu Val Val 
    1070                 1075                 1080             


Leu Val  Ala Thr Ile Leu Val  Ala Val Gly Val Phe  Asp Leu Thr 
    1085                 1090                 1095             


Pro Ser  Asn Met Gly Leu Ile  Ile Ser Ala Ala Ser  Phe Ile Pro 
    1100                 1105                 1110             


Glu Val  Met Ser Met Val Ala  Gln Ala Val Ala Glu  Leu Glu Asn 
    1115                 1120                 1125             


Cys Met  Asn Ala Thr Glu Arg  Ile Leu Tyr Tyr Lys  Asp Asn Ile 
    1130                 1135                 1140             


Pro Ala  Glu Ala Ala Arg Glu  Val Asp Gly Thr Glu  Leu Asp Gln 
    1145                 1150                 1155             


Arg Pro  Asn Trp Pro Glu Gln  Gly Ala Ile Ser Phe  Asn Asn Val 
    1160                 1165                 1170             


Ser Met  Lys Tyr Arg Asp Gly  Leu Pro Tyr Val Leu  Lys Ser Leu 
    1175                 1180                 1185             


Ser Val  Asp Phe Gln Gly Gly  His Lys Val Gly Ile  Cys Gly Arg 
    1190                 1195                 1200             


Thr Gly  Ala Gly Lys Ser Thr  Ile Leu Gln Thr Leu  Tyr Arg Ile 
    1205                 1210                 1215             


Val Glu  Leu Ala Glu Gly Ser  Ile Thr Ile Asp Gly  Val Asp Ile 
    1220                 1225                 1230             


Ser Thr  Ile Gly Leu His Gln  Leu Arg Ser Gln Leu  Ser Ile Ile 
    1235                 1240                 1245             


Pro Gln  Glu Pro Val Leu Phe  Leu Gly Thr Ile Arg  Ser Asn Leu 
    1250                 1255                 1260             


Asp Pro  Leu Glu Gln Tyr Ser  Asp Ala Glu Leu Trp  Gly Ser Leu 
    1265                 1270                 1275             


Arg Arg  Ser Gly Leu Leu Asp  Glu Gly Glu Thr Glu  Gly Lys Phe 
    1280                 1285                 1290             


His Leu  Asp Gln Lys Val Glu  Ala Asp Gly Ser Asn  Phe Ser Leu 
    1295                 1300                 1305             


Gly Glu  Arg Gln Leu Leu Thr  Leu Ala Arg Ala Leu  Leu Arg Asn 
    1310                 1315                 1320             


Thr Lys  Ile Leu Val Leu Asp  Glu Ala Thr Ser Asn  Val Asp Tyr 
    1325                 1330                 1335             


Lys Thr  Asp Lys Leu Val Gln  Glu Thr Ile Ser Arg  Glu Phe Gly 
    1340                 1345                 1350             


His Cys  Thr Ile Leu Cys Ile  Ala His Arg Leu Arg  Thr Ile Ala 
    1355                 1360                 1365             


Lys Tyr  Asp Arg Ile Leu Val  Leu Glu Ser Gly Glu  Ile Asn Gln 
    1370                 1375                 1380             


Tyr Asp  Thr Pro Trp Asn Leu  Tyr Asn Asp Lys Glu  Gly Ile Phe 
    1385                 1390                 1395             


Arg Gly  Met Cys Asp Thr Ser  Gly Leu Asn Glu Val  Asp Phe Asn 
    1400                 1405                 1410             


Lys 
    


<210>  4
<211>  500
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase

<400>  4

Met Thr Gln Ser Val Lys Val Val Glu Lys His Val Pro Ile Val Ile 
1               5                   10                  15      


Glu Lys Pro Ser Glu Lys Glu Glu Asp Thr Ser Ser Glu Asp Ser Ile 
            20                  25                  30          


Glu Leu Thr Val Gly Lys Gln Pro Lys Pro Val Thr Glu Thr Arg Ser 
        35                  40                  45              


Leu Asp Asp Leu Glu Ala Ile Met Lys Ala Gly Lys Thr Lys Leu Leu 
    50                  55                  60                  


Glu Asp His Glu Val Val Lys Leu Ser Leu Glu Gly Lys Leu Pro Leu 
65                  70                  75                  80  


Tyr Ala Leu Glu Lys Gln Leu Gly Asp Asn Thr Arg Ala Val Gly Ile 
                85                  90                  95      


Arg Arg Ser Ile Ile Ser Gln Gln Ser Asn Thr Lys Thr Leu Glu Thr 
            100                 105                 110         


Ser Lys Leu Pro Tyr Leu His Tyr Asp Tyr Asp Arg Val Phe Gly Ala 
        115                 120                 125             


Cys Cys Glu Asn Val Ile Gly Tyr Met Pro Leu Pro Val Gly Val Ala 
    130                 135                 140                 


Gly Pro Met Asn Ile Asp Gly Lys Asn Tyr His Ile Pro Met Ala Thr 
145                 150                 155                 160 


Thr Glu Gly Cys Leu Val Ala Ser Thr Met Arg Gly Cys Lys Ala Ile 
                165                 170                 175     


Asn Ala Gly Gly Gly Val Thr Thr Val Leu Thr Gln Asp Gly Met Thr 
            180                 185                 190         


Arg Gly Pro Cys Val Ser Phe Pro Ser Leu Lys Arg Ala Gly Ala Ala 
        195                 200                 205             


Lys Ile Trp Leu Asp Ser Glu Glu Gly Leu Lys Ser Met Arg Lys Ala 
    210                 215                 220                 


Phe Asn Ser Thr Ser Arg Phe Ala Arg Leu Gln Ser Leu His Ser Thr 
225                 230                 235                 240 


Leu Ala Gly Asn Leu Leu Phe Ile Arg Phe Arg Thr Thr Thr Gly Asp 
                245                 250                 255     


Ala Met Gly Met Asn Met Ile Ser Lys Gly Val Glu His Ser Leu Ala 
            260                 265                 270         


Val Met Val Lys Glu Tyr Gly Phe Pro Asp Met Asp Ile Val Ser Val 
        275                 280                 285             


Ser Gly Asn Tyr Cys Thr Asp Lys Lys Pro Ala Ala Ile Asn Trp Ile 
    290                 295                 300                 


Glu Gly Arg Gly Lys Ser Val Val Ala Glu Ala Thr Ile Pro Ala His 
305                 310                 315                 320 


Ile Val Lys Ser Val Leu Lys Ser Glu Val Asp Ala Leu Val Glu Leu 
                325                 330                 335     


Asn Ile Ser Lys Asn Leu Ile Gly Ser Ala Met Ala Gly Ser Val Gly 
            340                 345                 350         


Gly Phe Asn Ala His Ala Ala Asn Leu Val Thr Ala Ile Tyr Leu Ala 
        355                 360                 365             


Thr Gly Gln Asp Pro Ala Gln Asn Val Glu Ser Ser Asn Cys Ile Thr 
    370                 375                 380                 


Leu Met Ser Asn Val Asp Gly Asn Leu Leu Ile Ser Val Ser Met Pro 
385                 390                 395                 400 


Ser Ile Glu Val Gly Thr Ile Gly Gly Gly Thr Ile Leu Glu Pro Gln 
                405                 410                 415     


Gly Ala Met Leu Glu Met Leu Gly Val Arg Gly Pro His Ile Glu Thr 
            420                 425                 430         


Pro Gly Ala Asn Ala Gln Gln Leu Ala Arg Ile Ile Ala Ser Gly Val 
        435                 440                 445             


Leu Ala Ala Glu Leu Ser Leu Cys Ser Ala Leu Ala Ala Gly His Leu 
    450                 455                 460                 


Val Gln Ser His Met Thr His Asn Arg Ser Gln Ala Pro Thr Pro Ala 
465                 470                 475                 480 


Lys Gln Ser Gln Ala Asp Leu Gln Arg Leu Gln Asn Gly Ser Asn Ile 
                485                 490                 495     


Cys Ile Arg Ser 
            500 


<210>  5
<211>  327
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant geranylgeranyl-diphosphate synthase

<400>  5

Met Asp Tyr Asn Ser Ala Asp Phe Lys Glu Ile Trp Gly Lys Ala Ala 
1               5                   10                  15      


Asp Thr Ala Leu Leu Gly Pro Tyr Asn Tyr Leu Ala Asn Asn Arg Gly 
            20                  25                  30          


His Asn Ile Arg Glu His Leu Ile Ala Ala Phe Gly Ala Val Ile Lys 
        35                  40                  45              


Val Asp Lys Ser Asp Leu Glu Thr Ile Ser His Ile Thr Lys Ile Leu 
    50                  55                  60                  


His Asn Ser Ser Leu Leu Val Asp Asp Val Glu Asp Asn Ser Met Leu 
65                  70                  75                  80  


Arg Arg Gly Leu Pro Ala Ala His Cys Leu Phe Glu Val Pro Gln Thr 
                85                  90                  95      


Ile Asn Ser Val Asn Tyr Met Tyr Phe Val Ala Leu Gln Glu Val Leu 
            100                 105                 110         


Lys Leu Lys Ser Tyr Asp Ala Val Ser Ile Phe Thr Glu Glu Met Ile 
        115                 120                 125             


Asn Leu His Arg Gly Gln Gly Met Asp Leu Tyr Trp Arg Glu Thr Leu 
    130                 135                 140                 


Thr Cys Pro Ser Glu Asp Glu Tyr Leu Glu Met Val Val His Lys Thr 
145                 150                 155                 160 


Gly Gly Leu Phe Arg Leu Ala Leu Arg Leu Met Leu Ser Val Ala Ser 
                165                 170                 175     


Lys Gln Glu Asp His Glu Lys Ile Asn Phe Asp Leu Thr His Leu Thr 
            180                 185                 190         


Asp Thr Leu Gly Val Ile Tyr Gln Ile Leu Asp Asp Tyr Leu Asn Leu 
        195                 200                 205             


Gln Ser Thr Glu Leu Thr Glu Asn Lys Gly Phe Cys Glu Asp Ile Ser 
    210                 215                 220                 


Glu Gly Lys Phe Ser Phe Pro Leu Ile His Ser Ile Arg Thr Asn Pro 
225                 230                 235                 240 


Asp Asn His Glu Ile Leu Asn Ile Leu Lys Gln Arg Thr Ser Asp Ala 
                245                 250                 255     


Ser Leu Lys Lys Tyr Ala Val Asp Tyr Met Arg Thr Glu Thr Lys Ser 
            260                 265                 270         


Phe Asp Tyr Cys Leu Lys Arg Ile Gln Ala Met Ser Leu Lys Ala Ser 
        275                 280                 285             


Ser Tyr Ile Asp Asp Leu Ala Ala Ala Gly His Asp Val Ser Lys Leu 
    290                 295                 300                 


Arg Ala Ile Leu His Tyr Phe Val Ser Thr Ser Asp Cys Glu Glu Arg 
305                 310                 315                 320 


Lys Tyr Phe Glu Asp Ala Gln 
                325         


<210>  6
<211>  525
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant kaurenoic acid 13-hydroxylase

<400>  6

Met Glu Ser Leu Val Val His Thr Val Asn Ala Ile Trp Cys Ile Val 
1               5                   10                  15      


Ile Val Gly Ile Phe Ser Val Gly Tyr His Val Tyr Gly Arg Ala Val 
            20                  25                  30          


Val Glu Gln Trp Arg Met Arg Arg Ser Leu Lys Leu Gln Gly Val Lys 
        35                  40                  45              


Gly Pro Pro Pro Ser Ile Phe Asn Gly Asn Val Ser Glu Met Gln Arg 
    50                  55                  60                  


Ile Gln Ser Glu Ala Lys His Asn Ser Gly Asp Asn Ile Ile Ser His 
65                  70                  75                  80  


Asp Tyr Ser Ser Thr Leu Phe Pro His Phe Asp His Trp Arg Lys Gln 
                85                  90                  95      


Tyr Gly Arg Ile Tyr Thr Tyr Ser Thr Gly Leu Arg Gln His Leu Tyr 
            100                 105                 110         


Ile Asn His Pro Glu Met Val Lys Glu Leu Ser Gln Thr Asn Ser Leu 
        115                 120                 125             


Asp Leu Gly Arg Ile Thr His Val Thr Lys Arg Leu Ala Pro Ile Leu 
    130                 135                 140                 


Gly Asn Gly Ile Ile Thr Ser Asn Gly Pro His Trp Ala His Gln Arg 
145                 150                 155                 160 


Arg Ile Ile Ala Tyr Glu Phe Thr His Asp Lys Val Lys Gly Met Val 
                165                 170                 175     


Gly Leu Met Val Glu Ser Ala Met Pro Met Leu Asn Lys Trp Glu Glu 
            180                 185                 190         


Met Val Glu Ala Glu Gly Gly Met Gly Cys Asp Ile Arg Val Asp Glu 
        195                 200                 205             


Asp Leu Lys Asp Val Ser Ala Asp Val Ile Ala Lys Ala Cys Phe Gly 
    210                 215                 220                 


Ser Asn Phe Ser Lys Gly Lys Ala Ile Phe Ser Lys Ile Arg Asp Leu 
225                 230                 235                 240 


Leu Thr Ala Ile Thr Lys Arg Ser Val Leu Phe Arg Phe Asn Gly Phe 
                245                 250                 255     


Thr Asp Met Val Phe Gly Ser Lys Lys His Gly Asp Val Asp Ile Asp 
            260                 265                 270         


Ala Leu Glu Met Glu Leu Glu Ser Ser Ile Trp Glu Thr Val Lys Glu 
        275                 280                 285             


Arg Glu Arg Glu Cys Lys Asp Thr His Lys Lys Asp Leu Leu Gln Leu 
    290                 295                 300                 


Ile Leu Glu Gly Ala Met Arg Ser Cys Asp Gly Asn Leu Trp Asp Lys 
305                 310                 315                 320 


Ser Ala Tyr Arg Arg Phe Val Val Asp Asn Cys Lys Ser Ile Tyr Phe 
                325                 330                 335     


Ala Gly His Asp Ser Thr Ala Val Ser Val Ser Trp Cys Leu Met Leu 
            340                 345                 350         


Leu Ala Leu Asn Pro Ser Trp Gln Glu Lys Ile Arg Asp Glu Ile Leu 
        355                 360                 365             


Ser Ser Cys Lys Asn Gly Ile Pro Asp Ala Glu Ser Ile Pro Asn Leu 
    370                 375                 380                 


Lys Thr Val Thr Met Val Ile Gln Glu Thr Met Arg Leu Tyr Pro Pro 
385                 390                 395                 400 


Ala Pro Ile Val Gly Arg Glu Ala Ser Lys Asp Ile Arg Leu Gly Asp 
                405                 410                 415     


Leu Val Val Pro Lys Gly Val Cys Ile Trp Thr Leu Ile Pro Ala Leu 
            420                 425                 430         


His Arg Asp Pro Glu Ile Trp Gly Pro Asp Ala Asn Asp Phe Lys Pro 
        435                 440                 445             


Glu Arg Phe Ser Glu Gly Ile Ser Lys Ala Cys Lys Tyr Pro Gln Ala 
    450                 455                 460                 


Tyr Ile Pro Phe Gly Leu Gly Pro Arg Thr Cys Val Gly Lys Asn Phe 
465                 470                 475                 480 


Gly Met Met Glu Val Lys Val Leu Val Ser Leu Ile Val Ser Lys Phe 
                485                 490                 495     


Ser Phe Thr Leu Ser Pro Thr Tyr Gln His Ser Pro Ser His Lys Leu 
            500                 505                 510         


Leu Val Glu Pro Gln His Gly Val Val Ile Arg Val Val 
        515                 520                 525 


