                         SEQUENCE LISTING

<110>  Seattle Children's Research Institute
       bluebird bio, Inc.
       Rawlings, David J.
       Wang, Yupeng J.
       Khan, Iram F.
       Gay, Joel
 
<120>  BRUTON'S TYROSINE KINASE HOMING ENDONUCLEASE VARIANTS, 
       COMPOSITIONS, AND METHODS OF USE

<130>  BLBD-098/02WO 315698-2865

<150>  US 62/671,948
<151>  2018-05-15

<150>  US 62/663,982
<151>  2018-04-27

<160>  83    

<170>  PatentIn version 3.5

<210>  1
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  1

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Met Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Cys Val Met Glu Asn Lys Glu His Leu Lys Ile Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Ile Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  2
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi

<400>  2

Met Ala Tyr Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  3
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(3)
<223>  Any amino acid or absent

<400>  3

Xaa Xaa Xaa Met Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Val Phe 
    290                 295                 300             


<210>  4
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(4)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  4

Xaa Xaa Xaa Xaa Ser Arg Arg Glu Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  5
<211>  303
<212>  PRT
<213>  Ophiostoma novo-ulmi


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  5

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Ser Phe Leu Leu Arg Ile Arg Asn Asn 
            20                  25                  30          


Asn Lys Ser Ser Val Gly Tyr Ser Thr Glu Leu Gly Phe Gln Ile Thr 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Val Ile Ala Asn Ser Gly Asp Asn Ala Val Ser Leu Lys 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Leu Asn Trp Gly Leu Thr Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Ser Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Phe Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Glu Gly Cys Phe Phe Val Asn Leu Ile Lys Ser Lys Ser Lys Leu 
            180                 185                 190         


Gly Val Gln Val Gln Leu Val Phe Ser Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Lys Glu Lys Asn Lys Ser Glu Phe Ser Trp Leu Asp Phe Val Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  6
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK L4 V25


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  6

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Leu Leu Val Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp His Tyr Val Arg Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Tyr Val Asn Leu Val Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Thr Val Gln Leu Val Phe Gln Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  7
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL4 V34


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  7

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Leu Leu Val Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp His Tyr Val Arg Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Arg Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Tyr Val Asn Leu Val Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Thr Val Gln Leu Val Phe Gln Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Lys Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  8
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL V42


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  8

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Leu Leu Asp Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Arg Asn Ser Gly Asp Arg Tyr Val Ser Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Ser Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Tyr Val Asn Leu Val Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Thr Val Gln Leu Val Phe Gln Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Tyr Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  9
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL4 V5


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  9

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Leu Leu Val Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp His Tyr Val Arg Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Val Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Thr Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Val Phe Gly Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  10
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL4 V4


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  10

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Leu Leu Val Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp His Tyr Val Arg Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Thr Val Gln Leu Val Phe Gln Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  11
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL5N V3


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  11

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Leu Leu Asp Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp His Tyr Val Arg Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Thr Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Val Phe Gly Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  12
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL5N V7


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  12

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Met Leu Asp Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Arg Asn Ser Gly Asp Arg Tyr Val Ser Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Glu Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Thr Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Val Phe Gly Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  13
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL5 V12


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  13

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Ser Leu Val Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp Arg Ala Val Arg Leu Thr 
65                  70                  75                  80  


Val Thr Arg Phe Gly Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Thr Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Val Phe Gly Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  14
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL5 V17


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  14

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Ser Leu Val Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp Arg Ala Val Arg Leu Thr 
65                  70                  75                  80  


Val Thr Arg Phe Gly Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Thr Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Val Phe Gly Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  15
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL5 V18


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  15

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Leu Leu Val Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp His Tyr Val Arg Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Thr Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Val Phe Gly Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  16
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL5 V21


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  16

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Ser Leu Val Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Arg Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp Arg Ala Val Arg Leu Thr 
65                  70                  75                  80  


Val Thr Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Ile Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Ser Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Val Phe Gly Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  17
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthesized I-OnuI variant BTK EL5N V44


<220>
<221>  MOD_RES
<222>  (1)..(8)
<223>  Any amino acid or absent

<220>
<221>  MOD_RES
<222>  (302)..(303)
<223>  Any amino acid or absent

<400>  17

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ser Ile Asn Pro Trp Ile Leu Thr 
1               5                   10                  15      


Gly Phe Ala Asp Ala Glu Gly Trp Phe Leu Leu Asp Ile Arg Asn Ser 
            20                  25                  30          


Asn Thr Val Lys Val Gly Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu 
        35                  40                  45              


Leu His Asn Lys Asp Lys Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp 
    50                  55                  60                  


Lys Val Gly Lys Ile Ser Asn Ser Gly Asp His Tyr Val Arg Leu Thr 
65                  70                  75                  80  


Val Ser Arg Phe Glu Asp Leu Lys Val Ile Ile Asp His Phe Glu Lys 
                85                  90                  95      


Tyr Pro Leu Ile Thr Gln Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln 
            100                 105                 110         


Ala Phe Ser Leu Met Glu Asn Lys Glu His Leu Lys Glu Asn Gly Ile 
        115                 120                 125             


Lys Glu Leu Val Arg Ile Lys Ala Lys Met Asn Trp Gly Leu Asn Asp 
    130                 135                 140                 


Glu Leu Lys Lys Ala Phe Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu 
145                 150                 155                 160 


Val Asn Lys Asn Ile Pro Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser 
                165                 170                 175     


Gly Asp Gly Thr Phe Tyr Val Asn Leu Ile Lys Gly Lys Asn Thr Thr 
            180                 185                 190         


Arg Val Tyr Val Gln Leu Val Phe Gly Ile Thr Gln His Ile Lys Asp 
        195                 200                 205             


Lys Asn Leu Met Asn Ser Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile 
    210                 215                 220                 


Leu Glu Lys Asn Val Ser Glu Arg Ser Phe Leu Gln Phe Arg Val Thr 
225                 230                 235                 240 


Lys Phe Ser Asp Ile Asn Asp Lys Ile Ile Pro Val Phe Gln Glu Asn 
                245                 250                 255     


Thr Leu Ile Gly Val Lys Leu Glu Asp Phe Glu Asp Trp Cys Lys Val 
            260                 265                 270         


Ala Lys Leu Ile Glu Glu Lys Lys His Leu Thr Glu Ser Gly Leu Asp 
        275                 280                 285             


Glu Ile Lys Lys Ile Lys Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
    290                 295                 300             


<210>  18
<211>  873
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL I-OnuI variant BTK L4 V25 construct


<220>
<221>  MOD_RES
<222>  (872)..(873)
<223>  Any amino acid or absent

<400>  18

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu 
            580                 585                 590         


Gly Trp Phe Leu Leu Val Ile Arg Asn Ser Asn Thr Val Lys Val Gly 
        595                 600                 605             


Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu Leu His Asn Lys Asp Lys 
    610                 615                 620                 


Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Ser 
625                 630                 635                 640 


Asn Ser Gly Asp His Tyr Val Arg Leu Thr Val Ser Arg Phe Glu Asp 
                645                 650                 655     


Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln 
            660                 665                 670         


Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Leu Met Glu 
        675                 680                 685             


Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile 
    690                 695                 700                 


Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe 
705                 710                 715                 720 


Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro 
                725                 730                 735     


Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Tyr 
            740                 745                 750         


Val Asn Leu Val Lys Gly Lys Asn Thr Thr Arg Val Thr Val Gln Leu 
        755                 760                 765             


Val Phe Gln Ile Thr Gln His Ile Lys Asp Lys Asn Leu Met Asn Ser 
    770                 775                 780                 


Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Leu Glu Lys Asn Val Ser 
785                 790                 795                 800 


Glu Arg Ser Phe Leu Gln Phe Arg Val Thr Lys Phe Ser Asp Ile Asn 
                805                 810                 815     


Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys 
            820                 825                 830         


Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu 
        835                 840                 845             


Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys 
    850                 855                 860                 


Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
865                 870             


<210>  19
<211>  873
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL I-OnuI variant BTK EL4 V34 construct


<220>
<221>  MOD_RES
<222>  (872)..(873)
<223>  Any amino acid or absent

<400>  19

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu 
            580                 585                 590         


Gly Trp Phe Leu Leu Val Ile Arg Asn Ser Asn Thr Val Lys Val Gly 
        595                 600                 605             


Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu Leu His Asn Lys Asp Lys 
    610                 615                 620                 


Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Ser 
625                 630                 635                 640 


Asn Ser Gly Asp His Tyr Val Arg Leu Thr Val Ser Arg Phe Glu Asp 
                645                 650                 655     


Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln 
            660                 665                 670         


Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Leu Met Glu 
        675                 680                 685             


Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile 
    690                 695                 700                 


Arg Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe 
705                 710                 715                 720 


Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro 
                725                 730                 735     


Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Tyr 
            740                 745                 750         


Val Asn Leu Val Lys Gly Lys Asn Thr Thr Arg Val Thr Val Gln Leu 
        755                 760                 765             


Val Phe Gln Ile Thr Gln His Ile Lys Asp Lys Asn Leu Met Asn Ser 
    770                 775                 780                 


Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Leu Glu Lys Asn Val Ser 
785                 790                 795                 800 


Glu Arg Ser Phe Leu Gln Phe Arg Val Thr Lys Phe Ser Asp Ile Lys 
                805                 810                 815     


Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys 
            820                 825                 830         


Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu 
        835                 840                 845             


Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys 
    850                 855                 860                 


Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
865                 870             


<210>  20
<211>  873
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL I-OnuI variant BTK EL V42 construct


<220>
<221>  MOD_RES
<222>  (872)..(873)
<223>  Any amino acid or absent

<400>  20

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu 
            580                 585                 590         


Gly Trp Phe Leu Leu Asp Ile Arg Asn Ser Asn Thr Val Lys Val Gly 
        595                 600                 605             


Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu Leu His Asn Lys Asp Lys 
    610                 615                 620                 


Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Arg 
625                 630                 635                 640 


Asn Ser Gly Asp Arg Tyr Val Ser Leu Thr Val Ser Arg Phe Glu Asp 
                645                 650                 655     


Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln 
            660                 665                 670         


Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Leu Met Glu 
        675                 680                 685             


Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile 
    690                 695                 700                 


Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe 
705                 710                 715                 720 


Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Ser Ile Pro 
                725                 730                 735     


Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Tyr 
            740                 745                 750         


Val Asn Leu Val Lys Gly Lys Asn Thr Thr Arg Val Thr Val Gln Leu 
        755                 760                 765             


Val Phe Gln Ile Thr Gln His Ile Lys Asp Lys Tyr Leu Met Asn Ser 
    770                 775                 780                 


Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Leu Glu Lys Asn Val Ser 
785                 790                 795                 800 


Glu Arg Ser Phe Leu Gln Phe Arg Val Thr Lys Phe Ser Asp Ile Asn 
                805                 810                 815     


Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys 
            820                 825                 830         


Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu 
        835                 840                 845             


Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys 
    850                 855                 860                 


Leu Asn Met Asn Lys Gly Arg Xaa Xaa 
865                 870             


<210>  21
<211>  1112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL I-OnuI variant BTK L4 V25 TREX2 fusion 
       construct

<400>  21

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu 
            580                 585                 590         


Gly Trp Phe Leu Leu Val Ile Arg Asn Ser Asn Thr Val Lys Val Gly 
        595                 600                 605             


Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu Leu His Asn Lys Asp Lys 
    610                 615                 620                 


Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Ser 
625                 630                 635                 640 


Asn Ser Gly Asp His Tyr Val Arg Leu Thr Val Ser Arg Phe Glu Asp 
                645                 650                 655     


Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln 
            660                 665                 670         


Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Leu Met Glu 
        675                 680                 685             


Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile 
    690                 695                 700                 


Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe 
705                 710                 715                 720 


Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro 
                725                 730                 735     


Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Tyr 
            740                 745                 750         


Val Asn Leu Val Lys Gly Lys Asn Thr Thr Arg Val Thr Val Gln Leu 
        755                 760                 765             


Val Phe Gln Ile Thr Gln His Ile Lys Asp Lys Asn Leu Met Asn Ser 
    770                 775                 780                 


Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Leu Glu Lys Asn Val Ser 
785                 790                 795                 800 


Glu Arg Ser Phe Leu Gln Phe Arg Val Thr Lys Phe Ser Asp Ile Asn 
                805                 810                 815     


Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys 
            820                 825                 830         


Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu 
        835                 840                 845             


Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys 
    850                 855                 860                 


Leu Asn Met Asn Lys Gly Arg Val Phe Ala Ser Thr Gly Ser Glu Pro 
865                 870                 875                 880 


Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu 
                885                 890                 895     


Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu Phe Ala Val His 
            900                 905                 910         


Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser Gly Ser Leu Val 
        915                 920                 925             


Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Arg 
    930                 935                 940                 


Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu Ser Ser Glu Ser 
945                 950                 955                 960 


Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala Val Val Arg Thr 
                965                 970                 975     


Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile Cys Leu Val Ala 
            980                 985                 990         


His Asn Gly Phe Asp Tyr Asp Phe  Pro Leu Leu Cys Thr  Glu Leu Gln 
        995                 1000                 1005             


Arg Leu  Gly Ala His Leu Pro  Gln Asp Thr Val Cys  Leu Asp Thr 
    1010                 1015                 1020             


Leu Pro  Ala Leu Arg Gly Leu  Asp Arg Ala His Ser  His Gly Thr 
    1025                 1030                 1035             


Arg Ala  Gln Gly Arg Lys Ser  Tyr Ser Leu Ala Ser  Leu Phe His 
    1040                 1045                 1050             


Arg Tyr  Phe Gln Ala Glu Pro  Ser Ala Ala His Ser  Ala Glu Gly 
    1055                 1060                 1065             


Asp Val  His Thr Leu Leu Leu  Ile Phe Leu His Arg  Ala Pro Glu 
    1070                 1075                 1080             


Leu Leu  Ala Trp Ala Asp Glu  Gln Ala Arg Ser Trp  Ala His Ile 
    1085                 1090                 1095             


Glu Pro  Met Tyr Val Pro Pro  Asp Gly Pro Ser Leu  Glu Ala 
    1100                 1105                 1110         


<210>  22
<211>  1112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL I-OnuI variant BTK EL4 V34 TREX2 fusion 
       construct

<400>  22

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu 
            580                 585                 590         


Gly Trp Phe Leu Leu Val Ile Arg Asn Ser Asn Thr Val Lys Val Gly 
        595                 600                 605             


Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu Leu His Asn Lys Asp Lys 
    610                 615                 620                 


Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Ser 
625                 630                 635                 640 


Asn Ser Gly Asp His Tyr Val Arg Leu Thr Val Ser Arg Phe Glu Asp 
                645                 650                 655     


Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln 
            660                 665                 670         


Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Leu Met Glu 
        675                 680                 685             


Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile 
    690                 695                 700                 


Arg Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe 
705                 710                 715                 720 


Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Asn Ile Pro 
                725                 730                 735     


Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Tyr 
            740                 745                 750         


Val Asn Leu Val Lys Gly Lys Asn Thr Thr Arg Val Thr Val Gln Leu 
        755                 760                 765             


Val Phe Gln Ile Thr Gln His Ile Lys Asp Lys Asn Leu Met Asn Ser 
    770                 775                 780                 


Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Leu Glu Lys Asn Val Ser 
785                 790                 795                 800 


Glu Arg Ser Phe Leu Gln Phe Arg Val Thr Lys Phe Ser Asp Ile Lys 
                805                 810                 815     


Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys 
            820                 825                 830         


Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu 
        835                 840                 845             


Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys 
    850                 855                 860                 


Leu Asn Met Asn Lys Gly Arg Val Phe Ala Ser Thr Gly Ser Glu Pro 
865                 870                 875                 880 


Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu 
                885                 890                 895     


Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu Phe Ala Val His 
            900                 905                 910         


Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser Gly Ser Leu Val 
        915                 920                 925             


Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Arg 
    930                 935                 940                 


Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu Ser Ser Glu Ser 
945                 950                 955                 960 


Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala Val Val Arg Thr 
                965                 970                 975     


Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile Cys Leu Val Ala 
            980                 985                 990         


His Asn Gly Phe Asp Tyr Asp Phe  Pro Leu Leu Cys Thr  Glu Leu Gln 
        995                 1000                 1005             


Arg Leu  Gly Ala His Leu Pro  Gln Asp Thr Val Cys  Leu Asp Thr 
    1010                 1015                 1020             


Leu Pro  Ala Leu Arg Gly Leu  Asp Arg Ala His Ser  His Gly Thr 
    1025                 1030                 1035             


Arg Ala  Gln Gly Arg Lys Ser  Tyr Ser Leu Ala Ser  Leu Phe His 
    1040                 1045                 1050             


Arg Tyr  Phe Gln Ala Glu Pro  Ser Ala Ala His Ser  Ala Glu Gly 
    1055                 1060                 1065             


Asp Val  His Thr Leu Leu Leu  Ile Phe Leu His Arg  Ala Pro Glu 
    1070                 1075                 1080             


Leu Leu  Ala Trp Ala Asp Glu  Gln Ala Arg Ser Trp  Ala His Ile 
    1085                 1090                 1095             


Glu Pro  Met Tyr Val Pro Pro  Asp Gly Pro Ser Leu  Glu Ala 
    1100                 1105                 1110         


<210>  23
<211>  1112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Made in Lab - megaTAL I-OnuI variant BTK EL V42 TREX2 fusion 
       construct

<400>  23

Met Gly Ser Ala Pro Pro Lys Lys Lys Arg Lys Val Val Asp Leu Arg 
1               5                   10                  15      


Thr Leu Gly Tyr Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val 
            20                  25                  30          


Arg Ser Thr Val Ala Gln His His Glu Ala Leu Val Gly His Gly Phe 
        35                  40                  45              


Thr His Ala His Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly 
    50                  55                  60                  


Thr Val Ala Val Thr Tyr Gln His Ile Ile Thr Ala Leu Pro Glu Ala 
65                  70                  75                  80  


Thr His Glu Asp Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg 
                85                  90                  95      


Ala Leu Glu Ala Leu Leu Thr Asp Ala Gly Glu Leu Arg Gly Pro Pro 
            100                 105                 110         


Leu Gln Leu Asp Thr Gly Gln Leu Val Lys Ile Ala Lys Arg Gly Gly 
        115                 120                 125             


Val Thr Ala Met Glu Ala Val His Ala Ser Arg Asn Ala Leu Thr Gly 
    130                 135                 140                 


Ala Pro Leu Asn Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
145                 150                 155                 160 


Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
                165                 170                 175     


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
            180                 185                 190         


Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
        195                 200                 205             


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
    210                 215                 220                 


Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg 
225                 230                 235                 240 


Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val 
                245                 250                 255     


Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu Thr Val 
            260                 265                 270         


Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp 
        275                 280                 285             


Gln Val Val Ala Ile Ala Ser Asn Ile Gly Gly Lys Gln Ala Leu Glu 
    290                 295                 300                 


Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly Leu Thr 
305                 310                 315                 320 


Pro Asp Gln Val Val Ala Ile Ala Ser His Asp Gly Gly Lys Gln Ala 
                325                 330                 335     


Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp His Gly 
            340                 345                 350         


Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Gly Gly Gly Lys 
        355                 360                 365             


Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys Gln Asp 
    370                 375                 380                 


His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn Asn Gly 
385                 390                 395                 400 


Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val Leu Cys 
                405                 410                 415     


Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala Ser Asn 
            420                 425                 430         


Ile Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu Pro Val 
        435                 440                 445             


Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala Ile Ala 
    450                 455                 460                 


Ser Asn Asn Gly Gly Lys Gln Ala Leu Glu Thr Val Gln Arg Leu Leu 
465                 470                 475                 480 


Pro Val Leu Cys Gln Asp His Gly Leu Thr Pro Asp Gln Val Val Ala 
                485                 490                 495     


Ile Ala Ser Asn Gly Gly Gly Lys Gln Ala Leu Glu Ser Ile Val Ala 
            500                 505                 510         


Gln Leu Ser Arg Pro Asp Pro Ala Leu Ala Ala Leu Thr Asn Asp His 
        515                 520                 525             


Leu Val Ala Leu Ala Cys Leu Gly Gly Arg Pro Ala Met Asp Ala Val 
    530                 535                 540                 


Lys Lys Gly Leu Pro His Ala Pro Glu Leu Ile Arg Arg Val Asn Arg 
545                 550                 555                 560 


Arg Ile Gly Glu Arg Thr Ser His Arg Val Ala Ile Ser Arg Val Gly 
                565                 570                 575     


Gly Ser Ser Ile Asn Pro Trp Ile Leu Thr Gly Phe Ala Asp Ala Glu 
            580                 585                 590         


Gly Trp Phe Leu Leu Asp Ile Arg Asn Ser Asn Thr Val Lys Val Gly 
        595                 600                 605             


Tyr Arg Thr Leu Leu Ser Phe Gly Ile Glu Leu His Asn Lys Asp Lys 
    610                 615                 620                 


Ser Ile Leu Glu Asn Ile Gln Ser Thr Trp Lys Val Gly Lys Ile Arg 
625                 630                 635                 640 


Asn Ser Gly Asp Arg Tyr Val Ser Leu Thr Val Ser Arg Phe Glu Asp 
                645                 650                 655     


Leu Lys Val Ile Ile Asp His Phe Glu Lys Tyr Pro Leu Ile Thr Gln 
            660                 665                 670         


Lys Leu Gly Asp Tyr Lys Leu Phe Lys Gln Ala Phe Ser Leu Met Glu 
        675                 680                 685             


Asn Lys Glu His Leu Lys Glu Asn Gly Ile Lys Glu Leu Val Arg Ile 
    690                 695                 700                 


Lys Ala Lys Met Asn Trp Gly Leu Asn Asp Glu Leu Lys Lys Ala Phe 
705                 710                 715                 720 


Pro Glu Asn Ile Ser Lys Glu Arg Pro Leu Ile Asn Lys Ser Ile Pro 
                725                 730                 735     


Asn Leu Lys Trp Leu Ala Gly Phe Thr Ser Gly Asp Gly Ser Phe Tyr 
            740                 745                 750         


Val Asn Leu Val Lys Gly Lys Asn Thr Thr Arg Val Thr Val Gln Leu 
        755                 760                 765             


Val Phe Gln Ile Thr Gln His Ile Lys Asp Lys Tyr Leu Met Asn Ser 
    770                 775                 780                 


Leu Ile Thr Tyr Leu Gly Cys Gly Tyr Ile Leu Glu Lys Asn Val Ser 
785                 790                 795                 800 


Glu Arg Ser Phe Leu Gln Phe Arg Val Thr Lys Phe Ser Asp Ile Asn 
                805                 810                 815     


Asp Lys Ile Ile Pro Val Phe Gln Glu Asn Thr Leu Ile Gly Val Lys 
            820                 825                 830         


Leu Glu Asp Phe Glu Asp Trp Cys Lys Val Ala Lys Leu Ile Glu Glu 
        835                 840                 845             


Lys Lys His Leu Thr Glu Ser Gly Leu Asp Glu Ile Lys Lys Ile Lys 
    850                 855                 860                 


Leu Asn Met Asn Lys Gly Arg Val Phe Ala Ser Thr Gly Ser Glu Pro 
865                 870                 875                 880 


Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu Ala Thr Gly Leu 
                885                 890                 895     


Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu Phe Ala Val His 
            900                 905                 910         


Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser Gly Ser Leu Val 
        915                 920                 925             


Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met Cys Pro Glu Arg 
    930                 935                 940                 


Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu Ser Ser Glu Ser 
945                 950                 955                 960 


Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala Val Val Arg Thr 
                965                 970                 975     


Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile Cys Leu Val Ala 
            980                 985                 990         


His Asn Gly Phe Asp Tyr Asp Phe  Pro Leu Leu Cys Thr  Glu Leu Gln 
        995                 1000                 1005             


Arg Leu  Gly Ala His Leu Pro  Gln Asp Thr Val Cys  Leu Asp Thr 
    1010                 1015                 1020             


Leu Pro  Ala Leu Arg Gly Leu  Asp Arg Ala His Ser  His Gly Thr 
    1025                 1030                 1035             


Arg Ala  Gln Gly Arg Lys Ser  Tyr Ser Leu Ala Ser  Leu Phe His 
    1040                 1045                 1050             


Arg Tyr  Phe Gln Ala Glu Pro  Ser Ala Ala His Ser  Ala Glu Gly 
    1055                 1060                 1065             


Asp Val  His Thr Leu Leu Leu  Ile Phe Leu His Arg  Ala Pro Glu 
    1070                 1075                 1080             


Leu Leu  Ala Trp Ala Asp Glu  Gln Ala Arg Ser Trp  Ala His Ile 
    1085                 1090                 1095             


Glu Pro  Met Tyr Val Pro Pro  Asp Gly Pro Ser Leu  Glu Ala 
    1100                 1105                 1110         


<210>  24
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  24
atatcaagga cttggcctta ga                                                22


<210>  25
<211>  11
<212>  DNA
<213>  Homo sapiens

<400>  25
gaaaactgag t                                                            11


<210>  26
<211>  39
<212>  DNA
<213>  Homo sapiens

<400>  26
gaaaactgag tttcaagata tcaaggactt ggccttaga                              39


<210>  27
<211>  2613
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - I-OnuI variant BTK L4 V25 mRNA

<400>  27
augggauccg cgccacctaa gaagaaacgc aaagtcgtgg atctacgcac gctcggctac       60

agtcagcagc agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac      120

gaggcactgg tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg      180

gcagcgttag ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt gccagaggcg      240

acacacgaag acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc      300

ttgctcacgg atgcggggga gttgagaggt ccgccgttac agttggacac aggccaactt      360

gtgaagattg caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat      420

gcactgacgg gtgcccccct gaacctgacc ccggaccaag tggtggctat cgccagcaac      480

aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac      540

catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg      600

ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac      660

caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg      720

ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc      780

agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc      840

caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag      900

caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc      960

ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg     1020

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct     1080

atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg     1140

ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc     1200

ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc     1260

ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctggaa     1320

acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg     1380

gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg     1440

ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac     1500

ggtggcggca agcaagcgct cgaaagcatt gtggcccagc tgagccggcc tgatccggcg     1560

ttggccgcgt tgaccaacga ccacctcgtc gccttggcct gcctcggcgg acgtcctgcc     1620

atggatgcag tgaaaaaggg attgccgcac gcgccggaat tgatcagaag agtcaatcgc     1680

cgtattggcg aacgcacgtc ccatcgcgtt gcgatatcta gagtgggagg aagctccatc     1740

aacccatgga ttctgactgg tttcgctgat gccgaaggat ggttcttgct agttatccgg     1800

aactcaaaca cagttaaggt ggggtacagg actttgctga gcttcggtat cgaactgcac     1860

aacaaggaca aatcgattct ggagaatatc cagtcgactt ggaaggtcgg caagatcagt     1920

aacagcggcg accactatgt ccgtctgaca gtctctcgtt tcgaagattt gaaagtgatt     1980

atcgaccact tcgagaaata tccgctgatt acccagaaat tgggcgatta caagctgttt     2040

aaacaggcat tcagcctcat ggagaacaaa gaacatctca aggagaatgg gattaaggag     2100

ctcgtacgaa tcaaagctaa gatgaattgg ggtctcaatg acgaattgaa aaaagcattt     2160

ccagagaaca ttagcaaaga gcgccccctt atcaataaga acattccgaa tctcaaatgg     2220

ctggctggat tcacatctgg tgacggctct ttctacgtga atctagttaa gggaaaaaac     2280

acgacgagag taactgtgca gctggttttc caaatcacgc agcacatcaa agacaagaac     2340

ctgatgaatt cattgataac atacctaggc tgtggttata tcctagagaa gaacgtatct     2400

gagagaagtt ttctccagtt cagagtaact aaattcagcg atatcaacga caagatcatt     2460

ccggtattcc aggaaaatac tctgattggc gtcaaactcg aggactttga agattggtgc     2520

aaggttgcca aattgatcga agagaagaaa cacctgaccg aatccggttt ggatgagatt     2580

aagaaaatca agctgaacat gaacaaaggt cgt                                  2613


<210>  28
<211>  2613
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - I-OnuI variant BTK EL4 V34 mRNA

<400>  28
augggauccg cgccacctaa gaagaaacgc aaagtcgtgg atctacgcac gctcggctac       60

agtcagcagc agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac      120

gaggcactgg tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg      180

gcagcgttag ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt gccagaggcg      240

acacacgaag acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc      300

ttgctcacgg atgcggggga gttgagaggt ccgccgttac agttggacac aggccaactt      360

gtgaagattg caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat      420

gcactgacgg gtgcccccct gaacctgacc ccggaccaag tggtggctat cgccagcaac      480

aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac      540

catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg      600

ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac      660

caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg      720

ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc      780

agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc      840

caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag      900

caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc      960

ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg     1020

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct     1080

atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg     1140

ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc     1200

ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc     1260

ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctggaa     1320

acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg     1380

gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg     1440

ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac     1500

ggtggcggca agcaagcgct cgaaagcatt gtggcccagc tgagccggcc tgatccggcg     1560

ttggccgcgt tgaccaacga ccacctcgtc gccttggcct gcctcggcgg acgtcctgcc     1620

atggatgcag tgaaaaaggg attgccgcac gcgccggaat tgatcagaag agtcaatcgc     1680

cgtattggcg aacgcacgtc ccatcgcgtt gcgatatcta gagtgggagg aagctccatc     1740

aacccatgga ttctgactgg tttcgctgat gccgaaggat ggttcttgct agttatccgg     1800

aactcaaaca cagttaaggt ggggtacagg actttgctga gcttcggtat cgaactgcac     1860

aacaaggaca aatcgattct ggagaatatc cagtcgactt ggaaggtcgg caagatcagt     1920

aacagcggcg accactatgt ccgtctgaca gtctctcgtt tcgaagattt gaaagtgatt     1980

atcgaccact tcgagaaata tccgctgatt acccagaaat tgggcgatta caagctgttt     2040

aaacaggcat tcagcctcat ggagaacaaa gaacatctca aggagaatgg gattaaggag     2100

ctcgtacgaa tcagagctaa gatgaattgg ggtctcaatg acgaattgaa aaaagcattt     2160

ccagagaaca ttagcaaaga gcgccccctt atcaataaga acattccgaa tctcaaatgg     2220

ctggctggat tcacatctgg tgacggctct ttctacgtga atctagttaa gggaaaaaac     2280

acgacgagag taactgtgca gctggttttc caaatcacgc agcacatcaa agacaagaac     2340

ctgatgaatt cattgataac atacctaggc tgtggttata tcctagagaa gaacgtatct     2400

gagagaagtt ttctccagtt cagagtaact aaattcagcg atatcaagga caagatcatt     2460

ccggtattcc aggaaaatac tctgattggc gtcaaactcg aggactttga agattggtgc     2520

aaggttgcca aattgatcga agagaagaaa cacctgaccg aatccggttt ggatgagatt     2580

aagaaaatca agctgaacat gaacaaaggt cgt                                  2613


<210>  29
<211>  2613
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - I-OnuI variant BTK EL V42 mRNA

<400>  29
augggauccg cgccacctaa gaagaaacgc aaagtcgtgg atctacgcac gctcggctac       60

agtcagcagc agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac      120

gaggcactgg tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg      180

gcagcgttag ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt gccagaggcg      240

acacacgaag acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc      300

ttgctcacgg atgcggggga gttgagaggt ccgccgttac agttggacac aggccaactt      360

gtgaagattg caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat      420

gcactgacgg gtgcccccct gaacctgacc ccggaccaag tggtggctat cgccagcaac      480

aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac      540

catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg      600

ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac      660

caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg      720

ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc      780

agcaacattg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc      840

caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag      900

caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc      960

ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg     1020

cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct     1080

atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg     1140

ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc     1200

ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc     1260

ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctggaa     1320

acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg     1380

gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg     1440

ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac     1500

ggtggcggca agcaagcgct cgaaagcatt gtggcccagc tgagccggcc tgatccggcg     1560

ttggccgcgt tgaccaacga ccacctcgtc gccttggcct gcctcggcgg acgtcctgcc     1620

atggatgcag tgaaaaaggg attgccgcac gcgccggaat tgatcagaag agtcaatcgc     1680

cgtattggcg aacgcacgtc ccatcgcgtt gcgatatcta gagtgggagg aagctccatc     1740

aacccatgga ttctgactgg tttcgctgat gccgaaggat ggttcctgct agatatccgg     1800

aactcaaaca cagttaaggt ggggtacagg actttgctga gcttcggtat cgaactgcac     1860

aacaaggaca aatcgattct ggagaatatc cagtcgactt ggaaggtcgg caagatcaga     1920

aacagcggcg accgctatgt cagtctgacc gtctctcgtt tcgaagattt gaaagtgatt     1980

atcgaccact tcgagaaata tccgctgatt acccagaaat tgggcgatta caagctgttt     2040

aaacaggcat tcagcctcat ggagaacaaa gaacatctca aggagaatgg gattaaggag     2100

ctcgtacgaa tcaaagctaa gatgaattgg ggtctcaatg acgaattgaa aaaagcattt     2160

ccagagaaca ttagcaaaga gcgccccctt atcaataaga gcattccgaa tctcaaatgg     2220

ctggctggat tcacatctgg tgacggctct ttctacgtga atctagttaa gggaaaaaac     2280

acgacgagag taactgtgca gctggttttc caaatcacgc agcacatcaa agacaagtac     2340

ctgatgaatt cattgataac atacctaggc tgtggttata tcctagagaa gaacgtatct     2400

gagagaagtt ttctccagtt cagagtaact aaattcagcg atatcaacga caagatcatt     2460

ccggtattcc aggaaaatac tctgattggc gtcaaactcg aggactttga agattggtgc     2520

aaggttgcca aattgatcga agagaagaaa cacctgaccg aatccggttt ggatgagatt     2580

aagaaaatca agctgaacat gaacaaaggt cgt                                  2613


<210>  30
<211>  711
<212>  RNA
<213>  Mus musculus

<400>  30
augucugagc caccucgggc ugagaccuuu guauuccugg accuagaagc cacugggcuc       60

ccaaacaugg acccugagau ugcagagaua ucccuuuuug cuguucaccg cucuucccug      120

gagaacccag aacgggauga uucugguucc uuggugcugc cccguguucu ggacaagcuc      180

acacugugca ugugcccgga gcgccccuuu acugccaagg ccagugagau uacugguuug      240

agcagcgaaa gccugaugca cugcgggaag gcugguuuca auggcgcugu gguaaggaca      300

cugcagggcu uccuaagccg ccaggagggc cccaucugcc uuguggccca caauggcuuc      360

gauuaugacu ucccacugcu gugcacggag cuacaacguc ugggugccca ucugccccaa      420

gacacugucu gccuggacac acugccugca uugcggggcc uggaccgugc ucacagccac      480

ggcaccaggg cucaaggccg caaaagcuac agccuggcca gucucuucca ccgcuacuuc      540

caggcugaac ccagugcugc ccauucagca gaaggugaug ugcacacccu gcuucugauc      600

uuccugcauc gugcuccuga gcugcucgcc ugggcagaug agcaggcccg cagcugggcu      660

cauauugagc ccauguacgu gccaccugau gguccaagcc ucgaagccug a               711


<210>  31
<211>  236
<212>  PRT
<213>  Mus musculus

<400>  31

Met Ser Glu Pro Pro Arg Ala Glu Thr Phe Val Phe Leu Asp Leu Glu 
1               5                   10                  15      


Ala Thr Gly Leu Pro Asn Met Asp Pro Glu Ile Ala Glu Ile Ser Leu 
            20                  25                  30          


Phe Ala Val His Arg Ser Ser Leu Glu Asn Pro Glu Arg Asp Asp Ser 
        35                  40                  45              


Gly Ser Leu Val Leu Pro Arg Val Leu Asp Lys Leu Thr Leu Cys Met 
    50                  55                  60                  


Cys Pro Glu Arg Pro Phe Thr Ala Lys Ala Ser Glu Ile Thr Gly Leu 
65                  70                  75                  80  


Ser Ser Glu Ser Leu Met His Cys Gly Lys Ala Gly Phe Asn Gly Ala 
                85                  90                  95      


Val Val Arg Thr Leu Gln Gly Phe Leu Ser Arg Gln Glu Gly Pro Ile 
            100                 105                 110         


Cys Leu Val Ala His Asn Gly Phe Asp Tyr Asp Phe Pro Leu Leu Cys 
        115                 120                 125             


Thr Glu Leu Gln Arg Leu Gly Ala His Leu Pro Gln Asp Thr Val Cys 
    130                 135                 140                 


Leu Asp Thr Leu Pro Ala Leu Arg Gly Leu Asp Arg Ala His Ser His 
145                 150                 155                 160 


Gly Thr Arg Ala Gln Gly Arg Lys Ser Tyr Ser Leu Ala Ser Leu Phe 
                165                 170                 175     


His Arg Tyr Phe Gln Ala Glu Pro Ser Ala Ala His Ser Ala Glu Gly 
            180                 185                 190         


Asp Val His Thr Leu Leu Leu Ile Phe Leu His Arg Ala Pro Glu Leu 
        195                 200                 205             


Leu Ala Trp Ala Asp Glu Gln Ala Arg Ser Trp Ala His Ile Glu Pro 
    210                 215                 220                 


Met Tyr Val Pro Pro Asp Gly Pro Ser Leu Glu Ala 
225                 230                 235     


<210>  32
<211>  659
<212>  PRT
<213>  Homo sapiens

<400>  32

Met Ala Ala Val Ile Leu Glu Ser Ile Phe Leu Lys Arg Ser Gln Gln 
1               5                   10                  15      


Lys Lys Lys Thr Ser Pro Leu Asn Phe Lys Lys Arg Leu Phe Leu Leu 
            20                  25                  30          


Thr Val His Lys Leu Ser Tyr Tyr Glu Tyr Asp Phe Glu Arg Gly Arg 
        35                  40                  45              


Arg Gly Ser Lys Lys Gly Ser Ile Asp Val Glu Lys Ile Thr Cys Val 
    50                  55                  60                  


Glu Thr Val Val Pro Glu Lys Asn Pro Pro Pro Glu Arg Gln Ile Pro 
65                  70                  75                  80  


Arg Arg Gly Glu Glu Ser Ser Glu Met Glu Gln Ile Ser Ile Ile Glu 
                85                  90                  95      


Arg Phe Pro Tyr Pro Phe Gln Val Val Tyr Asp Glu Gly Pro Leu Tyr 
            100                 105                 110         


Val Phe Ser Pro Thr Glu Glu Leu Arg Lys Arg Trp Ile His Gln Leu 
        115                 120                 125             


Lys Asn Val Ile Arg Tyr Asn Ser Asp Leu Val Gln Lys Tyr His Pro 
    130                 135                 140                 


Cys Phe Trp Ile Asp Gly Gln Tyr Leu Cys Cys Ser Gln Thr Ala Lys 
145                 150                 155                 160 


Asn Ala Met Gly Cys Gln Ile Leu Glu Asn Arg Asn Gly Ser Leu Lys 
                165                 170                 175     


Pro Gly Ser Ser His Arg Lys Thr Lys Lys Pro Leu Pro Pro Thr Pro 
            180                 185                 190         


Glu Glu Asp Gln Ile Leu Lys Lys Pro Leu Pro Pro Glu Pro Ala Ala 
        195                 200                 205             


Ala Pro Val Ser Thr Ser Glu Leu Lys Lys Val Val Ala Leu Tyr Asp 
    210                 215                 220                 


Tyr Met Pro Met Asn Ala Asn Asp Leu Gln Leu Arg Lys Gly Asp Glu 
225                 230                 235                 240 


Tyr Phe Ile Leu Glu Glu Ser Asn Leu Pro Trp Trp Arg Ala Arg Asp 
                245                 250                 255     


Lys Asn Gly Gln Glu Gly Tyr Ile Pro Ser Asn Tyr Val Thr Glu Ala 
            260                 265                 270         


Glu Asp Ser Ile Glu Met Tyr Glu Trp Tyr Ser Lys His Met Thr Arg 
        275                 280                 285             


Ser Gln Ala Glu Gln Leu Leu Lys Gln Glu Gly Lys Glu Gly Gly Phe 
    290                 295                 300                 


Ile Val Arg Asp Ser Ser Lys Ala Gly Lys Tyr Thr Val Ser Val Phe 
305                 310                 315                 320 


Ala Lys Ser Thr Gly Asp Pro Gln Gly Val Ile Arg His Tyr Val Val 
                325                 330                 335     


Cys Ser Thr Pro Gln Ser Gln Tyr Tyr Leu Ala Glu Lys His Leu Phe 
            340                 345                 350         


Ser Thr Ile Pro Glu Leu Ile Asn Tyr His Gln His Asn Ser Ala Gly 
        355                 360                 365             


Leu Ile Ser Arg Leu Lys Tyr Pro Val Ser Gln Gln Asn Lys Asn Ala 
    370                 375                 380                 


Pro Ser Thr Ala Gly Leu Gly Tyr Gly Ser Trp Glu Ile Asp Pro Lys 
385                 390                 395                 400 


Asp Leu Thr Phe Leu Lys Glu Leu Gly Thr Gly Gln Phe Gly Val Val 
                405                 410                 415     


Lys Tyr Gly Lys Trp Arg Gly Gln Tyr Asp Val Ala Ile Lys Met Ile 
            420                 425                 430         


Lys Glu Gly Ser Met Ser Glu Asp Glu Phe Ile Glu Glu Ala Lys Val 
        435                 440                 445             


Met Met Asn Leu Ser His Glu Lys Leu Val Gln Leu Tyr Gly Val Cys 
    450                 455                 460                 


Thr Lys Gln Arg Pro Ile Phe Ile Ile Thr Glu Tyr Met Ala Asn Gly 
465                 470                 475                 480 


Cys Leu Leu Asn Tyr Leu Arg Glu Met Arg His Arg Phe Gln Thr Gln 
                485                 490                 495     


Gln Leu Leu Glu Met Cys Lys Asp Val Cys Glu Ala Met Glu Tyr Leu 
            500                 505                 510         


Glu Ser Lys Gln Phe Leu His Arg Asp Leu Ala Ala Arg Asn Cys Leu 
        515                 520                 525             


Val Asn Asp Gln Gly Val Val Lys Val Ser Asp Phe Gly Leu Ser Arg 
    530                 535                 540                 


Tyr Val Leu Asp Asp Glu Tyr Thr Ser Ser Val Gly Ser Lys Phe Pro 
545                 550                 555                 560 


Val Arg Trp Ser Pro Pro Glu Val Leu Met Tyr Ser Lys Phe Ser Ser 
                565                 570                 575     


Lys Ser Asp Ile Trp Ala Phe Gly Val Leu Met Trp Glu Ile Tyr Ser 
            580                 585                 590         


Leu Gly Lys Met Pro Tyr Glu Arg Phe Thr Asn Ser Glu Thr Ala Glu 
        595                 600                 605             


His Ile Ala Gln Gly Leu Arg Leu Tyr Arg Pro His Leu Ala Ser Glu 
    610                 615                 620                 


Lys Val Tyr Thr Ile Met Tyr Ser Cys Trp His Glu Lys Ala Asp Glu 
625                 630                 635                 640 


Arg Pro Thr Phe Lys Ile Leu Leu Ser Asn Ile Leu Asp Val Met Asp 
                645                 650                 655     


Glu Glu Ser 
            


<210>  33
<211>  7174
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - AAV plasmid construct, pAAV-BTK mTAL MND.GFP.PA

<400>  33
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac acgcgtatga      180

acagaggtgg catctatatc agtaagacag ttgcatcact tttgcatgat gctgtctaaa      240

agaactaatt taagctaaat ggggaaaagg tcagaaaaca acaactaccc cccccccacc      300

aaaacccacc aaaaaaaatt atgttttcaa ctttagaaca aatcttctat cctttgtagc      360

tcagtcagtg ggtgtgggca aaatcagttg ggcagcagtt agtgtgtgtc cagaactgca      420

ggtgcagcct ccatatcctt attagttccc ttggttacag accccagtgg gacaatgttt      480

gaaaaattat attcaccgtc taggaaattg ggaactgaaa gtccaatatc tgcctcagtg      540

gagttctggc acctgcatta tcccttctgg gtatatcaag atcaacagct gcacagatac      600

ttttgctttt cacagattct acacatatca tataaaggtg aatagtgtaa agctacctct      660

acaccttacc aagcacacag gtgcgtgcca tttaacatct agagcattcc attgccttat      720

acaagaactc agtttatatg agctcacaac atcgaaccaa tcccccccca attcagtgtg      780

catccattat acctgaaacc tgacagagct gggggctgtg ggaggaggtt ggtaggaaga      840

aattattttg tgagctgtgc acatttttgt tccatttgaa actaggtagc taggctgagg      900

gggaaccaag agggatgagg attaatgtcc tgggtcctca ggaactttca ttatcaacag      960

cacacaggtg aactccagaa agaagaagct atggccgcag tgattctgga gagcatcttt     1020

ctgaagcgat cccaacagaa aaagaaaaca tcacctctaa acttcaagaa gcgcctgttt     1080

ctcttgaccg tgcacaaact ctcctactat gagtatgact ttgaacgtgg ggtaagtttc     1140

tcgactatga aaactgagtt tcaagatatc aaggacgaac agagaaacag gagaatatgg     1200

gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagttgg     1260

aacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg     1320

gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca     1380

gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa     1440

tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctctat ataagcagag     1500

ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacttccata     1560

gaaggatctc gaggccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc     1620

catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg     1680

cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct     1740

gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg     1800

ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt     1860

ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa     1920

gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga     1980

cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat     2040

ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga     2100

cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt     2160

gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga     2220

gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat     2280

ggacgagctg tacaagtaaa ctagtgtcga ctgctttatt tgtgaaattt gtgatgctat     2340

tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca     2400

ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaattggcc ttagatcttt     2460

cttggggaag aggtaaattt tcgttggtag gaggagggga gtagaatgga cctaagttct     2520

ttcaaattca gcaaaatatt tcctagccta taactagcta aagccggaaa gtcaaaggtc     2580

ctaagaagcc acaaggaaaa tattaccatg gaatcttgga attgatgagc actcattaaa     2640

tgattgttga aaatgaaatc gaagagttgg aaattgcttc cttacttcct atgaggaagg     2700

tacatacagt cattcactct tccatggtat ttgccctcca tttggtagtc atagatttat     2760

agatctggaa ggattttttt ttcttccccc acatgacagg tcctggtgcc acctcacttt     2820

gttgaatgat tagataacaa aatctaatca tctggttgct taatccctct taatctttct     2880

ccattttctt cctcattcta cttctcagag aagaggcagt aagaagggtt caatagatgt     2940

tgagaagatc acttgtgttg aaacagtggt tcctgaaaaa aatcctcctc cagaaagaca     3000

gattccggta agaagagacc aatgtctgag atggggaaca gcagatttga agaaatttgc     3060

aacatttaaa ttctctgtaa atagactggt gatgctgtgc aacgtggaac acggtcaagt     3120

ttcctttaaa aattcttcac tctaccatat tggttataaa gaatcttagc ttctttcctt     3180

catattcaga acatctcact aaacatggaa aatttgttaa cacaaacttt taaatgatgc     3240

tatatctagt tttcaaactg gtcagagatc attgatttta ttccctcagt tctctcagga     3300

tcagatttag aggcttaagt aagtctgaat gtcataatcc tagggctctg agtcacatga     3360

tatcctttaa taccttacta tttattctct tctcactttc cggagcgaga gatctagagt     3420

agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc     3480

cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg     3540

cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgccagctgg cgtaatagcg     3600

aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgat     3660

tccgttgcaa tggctggcgg taatattgtt ctggatatta ccagcaaggc cgatagtttg     3720

agttcttcta ctcaggcaag tgatgttatt actaatcaaa gaagtattgc gacaacggtt     3780

aatttgcgtg atggacagac tcttttactc ggtggcctca ctgattataa aaacacttct     3840

caggattctg gcgtaccgtt cctgtctaaa atccctttaa tcggcctcct gtttagctcc     3900

cgctctgatt ctaacgagga aagcacgtta tacgtgctcg tcaaagcaac catagtacgc     3960

gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac     4020

acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt     4080

cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc     4140

tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc     4200

gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact     4260

cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg     4320

gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc     4380

gaattttaac aaaatattaa cgtttacaat ttaaatattt gcttatacaa tcttcctgtt     4440

tttggggctt ttctgattat caaccggggt acatatgatt gacatgctag ttttacgatt     4500

accgttcatc gattctcttg tttgctccag actctcaggc aatgacctga tagcctttgt     4560

agagacctct caaaaatagc taccctctcc ggcatgaatt tatcagctag aacggttgaa     4620

tatcatattg atggtgattt gactgtctcc ggcctttctc acccgtttga atctttacct     4680

acacattact caggcattgc atttaaaata tatgagggtt ctaaaaattt ttatccttgc     4740

gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt tggtacaacc     4800

gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt gccttgcctg     4860

tatgatttat tggatgttgg aatcgcctga tgcggtattt tctccttacg catctgtgcg     4920

gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa     4980

gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg     5040

catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac     5100

cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta     5160

atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg     5220

gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat     5280

aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc     5340

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa     5400

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac     5460

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga     5520

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag     5580

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca     5640

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca     5700

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa     5760

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc     5820

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa     5880

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag     5940

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct     6000

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac     6060

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa     6120

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt     6180

aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat     6240

ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg     6300

agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc     6360

ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg     6420

tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag     6480

cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact     6540

ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg     6600

gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc     6660

ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg     6720

aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg     6780

cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag     6840

ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc     6900

gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct     6960

ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc     7020

ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc     7080

gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac     7140

cgcctctccc cgcgcgttgg ccgattcatt aatg                                 7174


<210>  34
<211>  7308
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - AAV plasmid construct, 
       pAAV.BTK.1.0.1183jxn.MND.GFP.SV40pA

<400>  34
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac acgcgttcta      180

aaagaactaa tttaagctaa atggggaaaa ggtcagaaaa caacaactac ccccccccca      240

ccaaaaccca ccaaaaaaaa ttatgttttc aagggaactt tatttgtctt tctgtgtttc      300

agttacctaa attgaatcct tctggagtat tgtaggtttg gggaggctaa ataagttgtg      360

tttcataaat gaacagaggt ggcatctata tcagtaagac agttgcatca cttttgcatg      420

atgctgtcta aaagaactaa tttaagctaa atggggaaaa ggtcagaaaa caacaactac      480

ccccccccca ccaaaaccca ccaaaaaaaa ttatgttttc aactttagaa caaatcttct      540

atcctttgta gctcagtcag tgggtgtggg caaaatcagt tgggcagcag ttagtgtgtg      600

tccagaactg caggtgcagc ctccatatcc ttattagttc ccttggttac agaccccagt      660

gggacaatgt ttgaaaaatt atattcaccg tctaggaaat tgggaactga aagtccaata      720

tctgcctcag tggagttctg gcacctgcat tatcccttct gggtatatca agatcaacag      780

ctgcacagat acttttgctt ttcacagatt ctacacatat catataaagg tgaatagtgt      840

aaagctacct ctacacctta ccaagcacac aggtgcgtgc catttaacat ctagagcatt      900

ccattgcctt atacaagaac tcagtttata tgagctcaca acatcgaacc aatccccccc      960

caattcagtg tgcatccatt atacctgaaa cctgacagag ctgggggctg tgggaggagg     1020

ttggtaggaa gaaattattt tgtgagctgt gcacattttt gttccatttg aaactaggta     1080

gctaggctga gggggaacca agagggatga ggattaatgt cctgggtcct caggaacttt     1140

cattatcaac agcacacagg tgaactccag aaagaagaag ctatggccgc agtgattctg     1200

gagagcatct ttctgaagcg atcccgaaca gagaaacagg agaatatggg ccaaacagga     1260

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat     1320

atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag     1380

atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag     1440

ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt     1500

ctcgcttctg ttcgcgcgct tctgctcccc gagctctata taagcagagc tcgtttagtg     1560

aaccgtcaga tcgcctggag acgccatcca cgctgttttg acttccatag aaggatctcg     1620

aggccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg     1680

agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg     1740

ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct     1800

ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc     1860

acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca     1920

ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg     1980

acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc     2040

tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc     2100

agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc     2160

agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg     2220

acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc     2280

acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt     2340

acaagtaaac tagtgtcgac tgctttattt gtgaaatttg tgatgctatt gctttatttg     2400

taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc     2460

aggttcaggg ggaggtgtgg gaggtttttt aaaagctaaa gccggaaagt caaaggtcct     2520

aagaagccac aaggaaaata ttaccatgga atcttggaat tgatgagcac tcattaaatg     2580

attgttgaaa atgaaatcga agagttggaa attgcttcct tacttcctat gaggaaggta     2640

catacagtca ttcactcttc catggtattt gccctccatt tggtagtcat agatttatag     2700

atctggaagg attttttttt cttcccccac atgacaggtc ctggtgccac ctcactttgt     2760

tgaatgatta gataacaaaa tctaatcatc tggttgctta atccctctta atctttctcc     2820

attttcttcc tcattctact tctcagagaa gaggcagtaa gaagggttca atagatgttg     2880

agaagatcac ttgtgttgaa acagtggttc ctgaaaaaaa tcctcctcca gaaagacaga     2940

ttccggtaag aagagaccaa tgtctgagat ggggaacagc agatttgaag aaatttgcaa     3000

catttaaatt ctctgtaaat agactggtga tgctgtgcaa cgtggaacac ggtcaagttt     3060

cctttaaaaa ttcttcactc taccatattg gttataaaga atcttagctt ctttccttca     3120

tattcagaac atctcactaa acatggaaaa tttgttaaca caaactttta aatgatgcta     3180

tatctagttt tcaaactggt cagagatcat tgattttatt ccctcagttc tctcaggatc     3240

agatttagag gcttaagtaa gtctgaatgt cataatccta gggctctgag tcacatgata     3300

tcctttaata ccttactatt tattctcttc tcactttccg gagcgagaga cataaaacct     3360

actgattttt gagttcactt ttaaaaaata tatatcaatt tcagtatttt ctttttttct     3420

tttttttttc tttttttaga cagagtctcg ctctgttgcc caggctggaa tgcactggtg     3480

ccatcttggc tcactgcaac cttcacctcc cgggttcaag caattctcat gcctcagcct     3540

cccaagtcta gagtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta     3600

gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca     3660

aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgccag     3720

ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa     3780

tggcgaatgg cgattccgtt gcaatggctg gcggtaatat tgttctggat attaccagca     3840

aggccgatag tttgagttct tctactcagg caagtgatgt tattactaat caaagaagta     3900

ttgcgacaac ggttaatttg cgtgatggac agactctttt actcggtggc ctcactgatt     3960

ataaaaacac ttctcaggat tctggcgtac cgttcctgtc taaaatccct ttaatcggcc     4020

tcctgtttag ctcccgctct gattctaacg aggaaagcac gttatacgtg ctcgtcaaag     4080

caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc     4140

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc     4200

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg     4260

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca     4320

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc     4380

tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct     4440

tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa     4500

caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaat atttgcttat     4560

acaatcttcc tgtttttggg gcttttctga ttatcaaccg gggtacatat gattgacatg     4620

ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc aggcaatgac     4680

ctgatagcct ttgtagagac ctctcaaaaa tagctaccct ctccggcatg aatttatcag     4740

ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt tctcacccgt     4800

ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag ggttctaaaa     4860

atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag ggtcataatg     4920

tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat tttgctaatt     4980

ctttgccttg cctgtatgat ttattggatg ttggaatcgc ctgatgcggt attttctcct     5040

tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga     5100

tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc     5160

ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg     5220

tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct     5280

atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg     5340

gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc     5400

gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag     5460

tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt     5520

tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt     5580

gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga     5640

acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat     5700

tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga     5760

gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag     5820

tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg     5880

accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg     5940

ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt     6000

agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg     6060

gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc     6120

ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg     6180

tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac     6240

ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact     6300

gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa     6360

acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa     6420

aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg     6480

atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc     6540

gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac     6600

tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca     6660

ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt     6720

ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc     6780

ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg     6840

aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc     6900

cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac     6960

gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct     7020

ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc     7080

cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt     7140

tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac     7200

cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg     7260

cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatg                  7308


<210>  35
<211>  5718
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Made in Lab - AAV plasmid construct, pAAV 
       BTK.mTAL.ATG.coBTKV2.WPRE3.SV40pA

<400>  35
cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc       60

tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc      120

actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac acgcgtattc      180

accgtctagg aaattgggaa ctgaaagtcc aatatctgcc tcagtggagt tctggcacct      240

gcattatccc ttctgggtat atcaagatca acagctgcac agatactttt gcttttcaca      300

gattctacac atatcatata aaggtgaata gtgtaaagct acctctacac cttaccaagc      360

acacaggtgc gtgccattta acatctagag cattccattg ccttatacaa gaactcagtt      420

tatatgagct cacaacatcg aaccaatccc cccccaattc agtgtgcatc cattatacct      480

gaaacctgac agagctgggg gctgtgggag gaggttggta ggaagaaatt attttgtgag      540

ctgtgcacat ttttgttcca tttgaaacta ggtagctagg ctgaggggga accaagaggg      600

atgaggatta atgtcctggg tcctcaggaa ctttcattat caacagcaca caggtgaact      660

ccagaaagaa gaagctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat      720

cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga      780

gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc      840

cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta      900

ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca      960

ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt     1020

cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg     1080

caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc     1140

cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg     1200

cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct     1260

gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa     1320

gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga     1380

cgagctgtac aagggatccg gtgagggcag aggaagtctt ctaacatgcg gtgacgtgga     1440

ggagaatccg ggccccagaa gaggcagtaa gaagggttca atagatgttg agaagatcac     1500

ttgtgttgaa acagtggttc ctgaaaaaaa tcctcctcca gaaagacaga ttccggtaag     1560

aagagaccaa tgtctgagat ggggaacagc agatttgaag aaatttgcaa catttaaatt     1620

ctctgtaaat agactggtga tgctgtgcaa cgtggaacac ggtcaagttt cctttaaaaa     1680

ttcttcactc taccatattg gttataaaga atcttagctt ctttccttca tattcagaac     1740

atctcactaa acatggaaaa tttgttaaca caaactttta aatgatgcta tatctagttt     1800

tcaaactggt cagagatcat tgattttatt ccctcagttc tctcaggatc agatttagag     1860

gcttaagtaa gtctgaatgt cataatccta gggctctgag tcacatgata tcctttaata     1920

ccttactatt tattctcttc tcactttccg gagcgatcta gagtagataa gtagcatggc     1980

gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc     2040

gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg     2100

gcggcctcag tgagcgagcg agcgcgccag ctggcgtaat agcgaagagg cccgcaccga     2160

tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgattccgtt gcaatggctg     2220

gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct tctactcagg     2280

caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg cgtgatggac     2340

agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat tctggcgtac     2400

cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct gattctaacg     2460

aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg tagcggcgca     2520

ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta     2580

gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt     2640

caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac     2700

cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt     2760

tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga     2820

acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg     2880

gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata     2940

ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga     3000

ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt catcgattct     3060

cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagagac ctctcaaaaa     3120

tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat attgatggtg     3180

atttgactgt ctccggcctt tctcacccgt ttgaatcttt acctacacat tactcaggca     3240

ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt     3300

ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta gctttatgct     3360

ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat ttattggatg     3420

ttggaatcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat     3480

atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc     3540

gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca     3600

agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg     3660

cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat     3720

ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt     3780

atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct     3840

tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc     3900

cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa     3960

agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg     4020

taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt     4080

tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg     4140

catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac     4200

ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc     4260

ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa     4320

catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc     4380

aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt     4440

aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga     4500

taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa     4560

atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa     4620

gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa     4680

tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt     4740

ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt     4800

gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg     4860

agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt     4920

aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca     4980

agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac     5040

tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac     5100

atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct     5160

taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg     5220

gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca     5280

gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt     5340

aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta     5400

tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc     5460

gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc     5520

cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa     5580

ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag     5640

cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg     5700

ttggccgatt cattaatg                                                   5718


<210>  36
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  36

Gly Gly Gly 
1           


<210>  37
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  37

Asp Gly Gly Gly Ser 
1               5   


<210>  38
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  38

Thr Gly Glu Lys Pro 
1               5   


<210>  39
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  39

Gly Gly Arg Arg 
1               


<210>  40
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  40

Gly Gly Gly Gly Ser 
1               5   


<210>  41
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  41

Glu Gly Lys Ser Ser Gly Ser Gly Ser Glu Ser Lys Val Asp 
1               5                   10                  


<210>  42
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  42

Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg Ser 
1               5                   10                  15      


Leu Asp 
        


<210>  43
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  43

Gly Gly Arg Arg Gly Gly Gly Ser 
1               5               


<210>  44
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  44

Leu Arg Gln Arg Asp Gly Glu Arg Pro 
1               5                   


<210>  45
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  45

Leu Arg Gln Lys Asp Gly Gly Gly Ser Glu Arg Pro 
1               5                   10          


<210>  46
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary linker sequence

<400>  46

Leu Arg Gln Lys Asp Gly Gly Gly Ser Gly Gly Gly Ser Glu Arg Pro 
1               5                   10                  15      


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease


<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Xaa is any amino acid

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa = Gly or Ser

<400>  47

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  48
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  48

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  49
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cleavage sequence by TEV protease

<400>  49

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  50
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  50

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  51
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  51

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  52
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  52

Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10                  


<210>  53
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  53

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  54
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  54

Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  55
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  55

Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
1               5                   10              


<210>  56
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  56

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  57
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  57

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  58
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  58

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  59
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  59

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  60
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  60

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  61
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  61

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  62
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  62

Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  63
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  63

Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  64
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  64

Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly Pro 
1               5                   10                  


<210>  65
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  65

Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
1               5                   10                  15      


Pro 
    


<210>  66
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  66

Gln Leu Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  67
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  67

Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      


Asp Val Glu Ser Asn Pro Gly Pro 
            20                  


<210>  68
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  68

Val Thr Glu Leu Leu Tyr Arg Met Lys Arg Ala Glu Thr Tyr Cys Pro 
1               5                   10                  15      


Arg Pro Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys 
            20                  25                  30          


Ile Val Ala Pro Val Lys Gln Thr 
        35                  40  


<210>  69
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  69

Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro 
1               5                   10                  15      


Gly Pro 
        


<210>  70
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  70

Leu Leu Ala Ile His Pro Thr Glu Ala Arg His Lys Gln Lys Ile Val 
1               5                   10                  15      


Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
            20                  25                  30          


Asp Val Glu Ser Asn Pro Gly Pro 
        35                  40  


<210>  71
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Self-cleaving polypeptide comprising 2A site

<400>  71

Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      


Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          


Pro 
    


<210>  72
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Consensus Kozak sequence

<400>  72
gccrccatgg                                                              10


<210>  73
<211>  42
<212>  DNA
<213>  Homo sapiens

<400>  73
tatgaaaact gagtttcaag atatcaagga cttggcctta ga                          42


<210>  74
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  74
ccgcatagga tttggcctta gg                                                22


<210>  75
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  75
atgacaggga tatggcctta gg                                                22


<210>  76
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  76
ctgcacagga tttggcctca ga                                                22


<210>  77
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  77
cagcatagga tatagcctta cc                                                22


<210>  78
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  78
aggaacagga tttggcctta gc                                                22


<210>  79
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  79
gagataagga tatagcctta ga                                                22


<210>  80
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  80
ctgctgtgga tttggcctta ta                                                22


<210>  81
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  81
atgaaaggga tttggcctta aa                                                22


<210>  82
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  82
gggcacagga cttggcctta tt                                                22


<210>  83
<211>  22
<212>  DNA
<213>  Homo sapiens

<400>  83
atgccaagga tatgacctca tc                                                22


