                         SEQUENCE LISTING

<110>  Scharenberg, Andrew M.
       Rawlings, David J.
       Sommer, Karen
       West, Samuel
       Honaker, Yuchi Chiang
       Takeuchi, Ryo
 
<120>  Rapamycin Resistant Cells

<130>  SCRI.186WO

<150>  US 62/663,562
<151>  2018-04-27

<160>  58    

<170>  PatentIn version 3.5

<210>  1
<211>  90
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  naked FRB wild-type polypeptide

<400>  1

Met Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe 
1               5                   10                  15      


Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His 
            20                  25                  30          


Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn 
        35                  40                  45              


Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys 
    50                  55                  60                  


Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Thr Gln Ala Trp Asp Leu 
65                  70                  75                  80  


Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90  


<210>  2
<211>  90
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  naked FRB mutant polypeptide

<400>  2

Met Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe 
1               5                   10                  15      


Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His 
            20                  25                  30          


Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn 
        35                  40                  45              


Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys 
    50                  55                  60                  


Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala Trp Asp Leu 
65                  70                  75                  80  


Tyr Tyr His Val Phe Arg Arg Ile Ser Lys 
                85                  90  


<210>  3
<211>  4187
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  DISC vector DNA

<400>  3
gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc       60

cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt      120

aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc      180

tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc      240

ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc      300

tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgct agcaccggtg      360

ccgccaccat gcctctgggc ctgctgtggc tgggcctggc cctgctgggc gccctgcacg      420

cccaggccgg cgtgcaggtg gagacaatct ccccaggcga cggacgcaca ttccctaagc      480

ggggccagac ctgcgttgtg cactatacag gcatgctgga ggatggcaag aagtttgaca      540

gctcccggga tagaaacaag ccattcaagt ttatgctggg caagcaggaa gtgatcagag      600

gctgggagga gggcgtggcc cagatgtctg tgggccagag ggccaagctg accatcagcc      660

cagactacgc ctatggagca acaggccacc caggaatcat cccacctcac gccaccctgg      720

tgttcgatgt ggagctgctg aagctgggcg agggatccaa cacatcaaaa gagaacccct      780

ttctgttcgc attggaggcc gtagtcatat ctgttggatc catgggactt attatctccc      840

tgttgtgtgt gtacttctgg ctggaacgga ctatgcccag gatccccacg ctcaagaatc      900

tggaagatct cgtcacagaa taccatggta atttcagcgc ctggagcgga gtctctaagg      960

gtctggccga atccctccaa cccgattatt ctgaacggtt gtgcctcgta tccgaaatac     1020

caccaaaagg cggggctctg ggtgagggcc caggggcgag tccgtgcaat caacacagcc     1080

cgtattgggc ccctccttgt tatacgttga agcccgaaac tggaagcgga gctactaact     1140

tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg gcactgcccg     1200

tgaccgccct gctgctgcct ctggccctgc tgctgcacgc agcccggcct atcctgtggc     1260

acgagatgtg gcacgagggc ctggaggagg ccagcaggct gtattttggc gagcgcaacg     1320

tgaagggcat gttcgaggtg ctggagcctc tgcacgccat gatggagaga ggcccacaga     1380

ccctgaagga gacatccttt aaccaggcct atggacggga cctgatggag gcacaggagt     1440

ggtgcagaaa gtacatgaag tctggcaatg tgaaggacct gctgcaggcc tgggatctgt     1500

actatcacgt gtttcggaga atctccaagg gcaaagacac gattccgtgg cttgggcatc     1560

tgctcgttgg gctgagtggt gcgtttggtt tcatcatctt ggtctatctc ttgatcaatt     1620

gcagaaatac aggcccttgg ctgaaaaaag tgctcaagtg taataccccc gacccaagca     1680

agttcttctc ccagctttct tcagagcatg gaggcgatgt gcagaaatgg ctctcttcac     1740

cttttccctc ctcaagcttc tccccgggag ggctggcgcc cgagatttca cctcttgagg     1800

tacttgaacg agacaaggtt acccaacttc tccttcaaca ggataaggta cccgaacctg     1860

cgagccttag ctccaaccac tctcttacga gctgcttcac caatcaggga tacttctttt     1920

tccaccttcc cgatgcgctg gaaatcgaag cttgtcaagt ttactttacc tatgatccat     1980

atagcgagga agatcccgac gaaggagtcg ccggtgcgcc cacgggttcc tcaccccaac     2040

ctctccagcc tctctcagga gaagatgatg cttattgcac ttttcccagt agagacgatc     2100

tcctcctctt ttctccatct cttttggggg gaccttcccc cccttctacg gcacctggcg     2160

ggtctggtgc tggcgaggag cggatgccgc cgtccctcca ggagcgagta ccacgagatt     2220

gggatcccca gccacttgga ccccccaccc ccggcgtacc tgaccttgtc gattttcaac     2280

ctccccctga attggtgctg cgagaggctg gggaggaagt tccggacgct gggccgaggg     2340

agggcgtgtc ctttccatgg agtaggcctc caggtcaagg cgagtttagg gctctcaacg     2400

cgcggctgcc gttgaataca gacgcttatc tctcactgca ggaactgcaa ggtcaggacc     2460

caacacatct tgtaggatct ggtgctacta atttttctct tttgaagcaa gctggagatg     2520

ttgaagagaa ccccggtccg gagatgtggc atgagggtct ggaagaagcg tctcgactgt     2580

actttggtga gcgcaatgtg aagggcatgt ttgaagtcct cgaacccctt catgccatga     2640

tggaacgcgg accccagacc ttgaaggaga caagttttaa ccaagcttac ggaagagacc     2700

tgatggaagc ccaggaatgg tgcaggaaat acatgaaaag cgggaatgtg aaggacttgc     2760

tccaagcgtg ggacctgtac tatcatgtct ttaggcgcat tagtaagggc agcggcgcca     2820

ccaacttcag cctgctgaag caggccggcg acgtggagga gaaccccggc cccgtgagca     2880

agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg     2940

agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg     3000

agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg     3060

acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca     3120

tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact     3180

tcgaggacgg cggcgtggtg accgtgaccc aggactcctc tctgcaggac ggcgagttca     3240

tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga     3300

agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg     3360

gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga     3420

ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt     3480

tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg     3540

gccgccactc caccggcggc atggacgagc tgtacaagtg aactagtgtc gacaatcaac     3600

ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta     3660

cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt     3720

tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg     3780

ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg     3840

gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca     3900

cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca     3960

ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg     4020

ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag     4080

cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc     4140

gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgga                   4187


<210>  4
<211>  393
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  micro-DISC DNA (cytoplasmic tail only; codon diverged)

<400>  4
ccagcagctc tcggcaaaga cacgattccg tggcttgggc atctgctcgt tgggctgagc       60

ggtgcgtttg gtttcatcat cttggtctat ctcttgatca attgcagaaa tacaggccct      120

tggctgaaaa aagtgctcaa gtgtaatacc cccgacccaa gcaagttctt ctcccagctt      180

tcttcagagc atggaggcga tgtgcagaaa tggctctctt caccttttcc ctcctcaagc      240

ttctccccgg gagggctggc gcccgagatt tcacctcttg aggtacttga acgagacaag      300

gttacccaac ttctccttca acaggataag gtacccgaac ctgcgagcct tagcttgaat      360

acagacgctt atctctcact gcaggaactg caa                                   393


<210>  5
<211>  131
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  micro-DISC polypeptide (cytoplasmic tail only)

<400>  5

Pro Ala Ala Leu Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu Leu 
1               5                   10                  15      


Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu 
            20                  25                  30          


Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys 
        35                  40                  45              


Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His 
    50                  55                  60                  


Gly Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser 
65                  70                  75                  80  


Phe Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu 
                85                  90                  95      


Glu Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro 
            100                 105                 110         


Glu Pro Ala Ser Leu Ser Leu Asn Thr Asp Ala Tyr Leu Ser Leu Gln 
        115                 120                 125             


Glu Leu Gln 
    130     


<210>  6
<211>  108
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  FKBP CISC domain

<400>  6

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  


Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      


Leu Val Phe Asp Val Glu Leu Leu Lys Leu Gly Glu 
            100                 105             


<210>  7
<211>  245
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Entire micro-DISC polypeptide (FRB-truncated IL2Rbeta)

<400>  7

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly Leu 
            20                  25                  30          


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        35                  40                  45              


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    50                  55                  60                  


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
65                  70                  75                  80  


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys 
                85                  90                  95      


Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile 
            100                 105                 110         


Ser Lys Pro Ala Ala Leu Gly Lys Asp Thr Ile Pro Trp Leu Gly His 
        115                 120                 125             


Leu Leu Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr 
    130                 135                 140                 


Leu Leu Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu 
145                 150                 155                 160 


Lys Cys Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser 
                165                 170                 175     


Glu His Gly Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser 
            180                 185                 190         


Ser Ser Phe Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu 
        195                 200                 205             


Val Leu Glu Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys 
    210                 215                 220                 


Val Pro Glu Pro Ala Ser Leu Ser Leu Asn Thr Asp Ala Tyr Leu Ser 
225                 230                 235                 240 


Leu Gln Glu Leu Gln 
                245 


<210>  8
<211>  3623
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  micro-DISC vector DNA

<400>  8
gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc       60

cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt      120

aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc      180

tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc      240

ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc      300

tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgct agcaccggtg      360

ccgccaccat gcctctgggc ctgctgtggc tgggcctggc cctgctgggc gccctgcacg      420

cccaggccgg cgtgcaggtg gagacaatct ccccaggcga cggacgcaca ttccctaagc      480

ggggccagac ctgcgttgtg cactatacag gcatgctgga ggatggcaag aagtttgaca      540

gctcccggga tagaaacaag ccattcaagt ttatgctggg caagcaggaa gtgatcagag      600

gctgggagga gggcgtggcc cagatgtctg tgggccagag ggccaagctg accatcagcc      660

cagactacgc ctatggagca acaggccacc caggaatcat cccacctcac gccaccctgg      720

tgttcgatgt ggagctgctg aagctgggcg agggatccaa cacatcaaaa gagaacccct      780

ttctgttcgc attggaggcc gtagtcatat ctgttggatc catgggactt attatctccc      840

tgttgtgtgt gtacttctgg ctggaacgga ctatgcccag gatccccacg ctcaagaatc      900

tggaagatct cgtcacagaa taccatggta atttcagcgc ctggagcgga gtctctaagg      960

gtctggccga atccctccaa cccgattatt ctgaacggtt gtgcctcgta tccgaaatac     1020

caccaaaagg cggggctctg ggtgagggcc caggggcgag tccgtgcaat caacacagcc     1080

cgtattgggc ccctccttgt tatacgttga agcccgaaac tggaagcgga gctactaact     1140

tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg gcactgcccg     1200

tgaccgccct gctgctgcct ctggccctgc tgctgcacgc agcccggcct atcctgtggc     1260

acgagatgtg gcacgagggc ctggaggagg ccagcaggct gtattttggc gagcgcaacg     1320

tgaagggcat gttcgaggtg ctggagcctc tgcacgccat gatggagaga ggcccacaga     1380

ccctgaagga gacatccttt aaccaggcct atggacggga cctgatggag gcacaggagt     1440

ggtgcagaaa gtacatgaag tctggcaatg tgaaggacct gctgcaggcc tgggatctgt     1500

actatcacgt gtttcggaga atctccaagg gcaaagacac gattccgtgg cttgggcatc     1560

tgctcgttgg gctgagtggt gcgtttggtt tcatcatctt ggtctatctc ttgatcaatt     1620

gcagaaatac aggcccttgg ctgaaaaaag tgctcaagtg taataccccc gacccaagca     1680

agttcttctc ccagctttct tcagagcatg gaggcgatgt gcagaaatgg ctctcttcac     1740

cttttccctc ctcaagcttc tccccgggag ggctggcgcc cgagatttca cctcttgagg     1800

tacttgaacg agacaaggtt acccaacttc tccttcaaca ggataaggta cccgaacctg     1860

cgagccttag cttgaataca gacgcttatc tctcactgca ggaactgcaa ggatctggtg     1920

ctactaattt ttctcttttg aagcaagctg gagatgttga agagaacccc ggtccggaga     1980

tgtggcatga gggtctggaa gaagcgtctc gactgtactt tggtgagcgc aatgtgaagg     2040

gcatgtttga agtcctcgaa ccccttcatg ccatgatgga acgcggaccc cagaccttga     2100

aggagacaag ttttaaccaa gcttacggaa gagacctgat ggaagcccag gaatggtgca     2160

ggaaatacat gaaaagcggg aatgtgaagg acttgctcca agcgtgggac ctgtactatc     2220

atgtctttag gcgcattagt aagggcagcg gcgccaccaa cttcagcctg ctgaagcagg     2280

ccggcgacgt ggaggagaac cccggccccg tgagcaaggg cgaggaggat aacatggcca     2340

tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt     2400

tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga     2460

aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt     2520

acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct     2580

tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg     2640

tgacccagga ctcctctctg caggacggcg agttcatcta caaggtgaag ctgcgcggca     2700

ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct     2760

ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc     2820

tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg     2880

tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg     2940

actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg     3000

acgagctgta caagtgaact agtgtcgaca atcaacctct ggattacaaa atttgtgaaa     3060

gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa     3120

tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat     3180

cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt     3240

gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc     3300

tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc     3360

ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg     3420

ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga     3480

cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc     3540

tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc     3600

tttgggccgc ctccccgcct gga                                             3623


<210>  9
<211>  251
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rgamma-CISC polypeptide

<400>  9

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Gly Glu 
        115                 120                 125             


Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu Glu Ala 
    130                 135                 140                 


Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
145                 150                 155                 160 


Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys 
                165                 170                 175     


Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp 
            180                 185                 190         


Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser 
        195                 200                 205             


Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu 
    210                 215                 220                 


Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp 
225                 230                 235                 240 


Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr 
                245                 250     


<210>  10
<211>  352
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rgamma-CISC polypeptide

<400>  10

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        115                 120                 125             


Gly Gly Ser Gln Asn Leu Val Ile Pro Trp Ala Pro Glu Asn Leu Thr 
    130                 135                 140                 


Leu His Lys Leu Ser Glu Ser Gln Leu Glu Leu Asn Trp Asn Asn Arg 
145                 150                 155                 160 


Phe Leu Asn His Cys Leu Glu His Leu Val Gln Tyr Arg Thr Asp Trp 
                165                 170                 175     


Asp His Ser Trp Thr Glu Gln Ser Val Asp Tyr Arg His Lys Phe Ser 
            180                 185                 190         


Leu Pro Ser Val Asp Gly Gln Lys Arg Tyr Thr Phe Arg Val Arg Ser 
        195                 200                 205             


Arg Phe Asn Pro Leu Cys Gly Ser Ala Gln His Trp Ser Glu Trp Ser 
    210                 215                 220                 


His Pro Ile His Trp Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu 
225                 230                 235                 240 


Phe Ala Leu Glu Ala Val Val Ile Ser Val Gly Ser Met Gly Leu Ile 
                245                 250                 255     


Ile Ser Leu Leu Cys Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg 
            260                 265                 270         


Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly 
        275                 280                 285             


Asn Phe Ser Ala Trp Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu 
    290                 295                 300                 


Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro 
305                 310                 315                 320 


Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln 
                325                 330                 335     


His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr 
            340                 345                 350         


<210>  11
<211>  349
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rgamma-CISC polypeptide

<400>  11

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        115                 120                 125             


Gln Asn Leu Val Ile Pro Trp Ala Pro Glu Asn Leu Thr Leu His Lys 
    130                 135                 140                 


Leu Ser Glu Ser Gln Leu Glu Leu Asn Trp Asn Asn Arg Phe Leu Asn 
145                 150                 155                 160 


His Cys Leu Glu His Leu Val Gln Tyr Arg Thr Asp Trp Asp His Ser 
                165                 170                 175     


Trp Thr Glu Gln Ser Val Asp Tyr Arg His Lys Phe Ser Leu Pro Ser 
            180                 185                 190         


Val Asp Gly Gln Lys Arg Tyr Thr Phe Arg Val Arg Ser Arg Phe Asn 
        195                 200                 205             


Pro Leu Cys Gly Ser Ala Gln His Trp Ser Glu Trp Ser His Pro Ile 
    210                 215                 220                 


His Trp Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu 
225                 230                 235                 240 


Glu Ala Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu 
                245                 250                 255     


Leu Cys Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr 
            260                 265                 270         


Leu Lys Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser 
        275                 280                 285             


Ala Trp Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp 
    290                 295                 300                 


Tyr Ser Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly 
305                 310                 315                 320 


Ala Leu Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro 
                325                 330                 335     


Tyr Trp Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr 
            340                 345                 


<210>  12
<211>  251
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rgamma-CISC polypeptide

<400>  12

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        115                 120                 125             


Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu Glu Ala 
    130                 135                 140                 


Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
145                 150                 155                 160 


Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys 
                165                 170                 175     


Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp 
            180                 185                 190         


Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser 
        195                 200                 205             


Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu 
    210                 215                 220                 


Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp 
225                 230                 235                 240 


Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr 
                245                 250     


<210>  13
<211>  429
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rbeta-CISC polypeptide

<400>  13

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly Leu 
            20                  25                  30          


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        35                  40                  45              


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    50                  55                  60                  


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
65                  70                  75                  80  


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys 
                85                  90                  95      


Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile 
            100                 105                 110         


Ser Lys Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu Leu Val Gly 
        115                 120                 125             


Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn 
    130                 135                 140                 


Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr 
145                 150                 155                 160 


Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly Gly 
                165                 170                 175     


Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe Ser 
            180                 185                 190         


Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu Arg 
        195                 200                 205             


Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu Pro 
    210                 215                 220                 


Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn Gln 
225                 230                 235                 240 


Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala Cys 
                245                 250                 255     


Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp Glu 
            260                 265                 270         


Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln Pro 
        275                 280                 285             


Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp Asp 
    290                 295                 300                 


Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro Ser 
305                 310                 315                 320 


Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro Ser 
                325                 330                 335     


Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly Pro 
            340                 345                 350         


Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro Glu 
        355                 360                 365             


Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro Arg 
    370                 375                 380                 


Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu Phe 
385                 390                 395                 400 


Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu Ser 
                405                 410                 415     


Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
            420                 425                 


<210>  14
<211>  543
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rbeta-CISC polypeptide

<400>  14

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly Leu 
            20                  25                  30          


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        35                  40                  45              


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    50                  55                  60                  


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
65                  70                  75                  80  


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys 
                85                  90                  95      


Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile 
            100                 105                 110         


Ser Lys Gly Gly Ser Lys Pro Phe Glu Asn Leu Arg Leu Met Ala Pro 
        115                 120                 125             


Ile Ser Leu Gln Val Val His Val Glu Thr His Arg Cys Asn Ile Ser 
    130                 135                 140                 


Trp Glu Ile Ser Gln Ala Ser His Tyr Phe Glu Arg His Leu Glu Phe 
145                 150                 155                 160 


Glu Ala Arg Thr Leu Ser Pro Gly His Thr Trp Glu Glu Ala Pro Leu 
                165                 170                 175     


Leu Thr Leu Lys Gln Lys Gln Glu Trp Ile Cys Leu Glu Thr Leu Thr 
            180                 185                 190         


Pro Asp Thr Gln Tyr Glu Phe Gln Val Arg Val Lys Pro Leu Gln Gly 
        195                 200                 205             


Glu Phe Thr Thr Trp Ser Pro Trp Ser Gln Pro Leu Ala Phe Arg Thr 
    210                 215                 220                 


Lys Pro Ala Ala Leu Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu 
225                 230                 235                 240 


Leu Val Gly Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu 
                245                 250                 255     


Leu Ile Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys 
            260                 265                 270         


Cys Asn Thr Pro Asp Pro Ser Lys Phe Phe Gln Leu Ser Ser Glu His 
        275                 280                 285             


Gly Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser 
    290                 295                 300                 


Phe Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu 
305                 310                 315                 320 


Glu Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro 
                325                 330                 335     


Glu Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr 
            340                 345                 350         


Asn Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu 
        355                 360                 365             


Ala Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro 
    370                 375                 380                 


Asp Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu 
385                 390                 395                 400 


Gln Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg 
                405                 410                 415     


Asp Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro 
            420                 425                 430         


Pro Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro 
        435                 440                 445             


Pro Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu 
    450                 455                 460                 


Gly Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro 
465                 470                 475                 480 


Pro Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly 
                485                 490                 495     


Pro Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly 
            500                 505                 510         


Glu Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr 
        515                 520                 525             


Leu Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
    530                 535                 540             


<210>  15
<211>  541
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rbeta-CISC polypeptide

<400>  15

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly Leu 
            20                  25                  30          


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        35                  40                  45              


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    50                  55                  60                  


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
65                  70                  75                  80  


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys 
                85                  90                  95      


Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile 
            100                 105                 110         


Ser Lys Lys Pro Phe Glu Asn Leu Arg Leu Met Ala Pro Ile Ser Leu 
        115                 120                 125             


Gln Val Val His Val Glu Thr His Arg Cys Asn Ile Ser Trp Glu Ile 
    130                 135                 140                 


Ser Gln Ala Ser His Tyr Phe Glu Arg His Leu Glu Phe Glu Ala Arg 
145                 150                 155                 160 


Thr Leu Ser Pro Gly His Thr Trp Glu Glu Ala Pro Leu Leu Thr Leu 
                165                 170                 175     


Lys Gln Lys Gln Glu Trp Ile Cys Leu Glu Thr Leu Thr Pro Asp Thr 
            180                 185                 190         


Gln Tyr Glu Phe Gln Val Arg Val Lys Pro Leu Gln Gly Glu Phe Thr 
        195                 200                 205             


Thr Trp Ser Pro Trp Ser Gln Pro Leu Ala Phe Arg Thr Lys Pro Ala 
    210                 215                 220                 


Ala Leu Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu Leu Val Gly 
225                 230                 235                 240 


Leu Ser Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn 
                245                 250                 255     


Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr 
            260                 265                 270         


Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly Gly 
        275                 280                 285             


Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe Ser 
    290                 295                 300                 


Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu Arg 
305                 310                 315                 320 


Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu Pro 
                325                 330                 335     


Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn Gln 
            340                 345                 350         


Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala Cys 
        355                 360                 365             


Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp Glu 
    370                 375                 380                 


Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln Pro 
385                 390                 395                 400 


Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp Asp 
                405                 410                 415     


Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro Ser 
            420                 425                 430         


Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro Ser 
        435                 440                 445             


Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly Pro 
    450                 455                 460                 


Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro Glu 
465                 470                 475                 480 


Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro Arg 
                485                 490                 495     


Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu Phe 
            500                 505                 510         


Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu Ser 
        515                 520                 525             


Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
    530                 535                 540     


<210>  16
<211>  379
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL7Ralpha-CISC polypeptide

<400>  16

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly Leu 
            20                  25                  30          


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        35                  40                  45              


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    50                  55                  60                  


Thr Leu Lys Glu Thr Ser Trp Leu Gly His Leu Leu Val Gly Leu Ser 
65                  70                  75                  80  


Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn Cys Arg 
                85                  90                  95      


Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr Pro Asp 
            100                 105                 110         


Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly Gly Asp Val 
        115                 120                 125             


Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe Ser Pro Gly 
    130                 135                 140                 


Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu Arg Asp Lys 
145                 150                 155                 160 


Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu Pro Ala Ser 
                165                 170                 175     


Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn Gln Gly Tyr 
            180                 185                 190         


Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala Cys Gln Val 
        195                 200                 205             


Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp Glu Gly Val 
    210                 215                 220                 


Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln Pro Leu Ser 
225                 230                 235                 240 


Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp Asp Leu Leu 
                245                 250                 255     


Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro Ser Thr Ala 
            260                 265                 270         


Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro Ser Leu Gln 
        275                 280                 285             


Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly Pro Pro Thr 
    290                 295                 300                 


Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro Glu Leu Val 
305                 310                 315                 320 


Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro Arg Glu Gly 
                325                 330                 335     


Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu Phe Arg Ala 
            340                 345                 350         


Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu Ser Leu Gln 
        355                 360                 365             


Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
    370                 375                 


<210>  17
<211>  345
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL7Ralpha-CISC polypeptide

<400>  17

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ile Leu Trp His Glu Met Trp His Glu Gly Leu 
            20                  25                  30          


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        35                  40                  45              


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    50                  55                  60                  


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
65                  70                  75                  80  


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys 
                85                  90                  95      


Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile 
            100                 105                 110         


Ser Lys Gly Glu Ile Asn Asn Ser Ser Gly Glu Met Asp Pro Ile Leu 
        115                 120                 125             


Leu Thr Ile Ser Ile Leu Ser Phe Phe Ser Val Ala Leu Leu Val Ile 
    130                 135                 140                 


Leu Ala Cys Val Leu Trp Lys Lys Arg Ile Lys Pro Ile Val Trp Pro 
145                 150                 155                 160 


Ser Leu Pro Asp His Lys Lys Thr Leu Glu His Leu Cys Lys Lys Pro 
                165                 170                 175     


Arg Lys Asn Leu Asn Val Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys 
            180                 185                 190         


Gln Ile His Arg Val Asp Asp Ile Gln Ala Arg Asp Glu Val Glu Gly 
        195                 200                 205             


Phe Leu Gln Asp Thr Phe Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln 
    210                 215                 220                 


Arg Leu Gly Gly Asp Val Gln Ser Pro Asn Cys Pro Ser Glu Asp Val 
225                 230                 235                 240 


Val Ile Thr Pro Glu Ser Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu 
                245                 250                 255     


Ala Gly Asn Val Ser Ala Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg 
            260                 265                 270         


Ser Leu Asp Cys Arg Glu Ser Gly Lys Asn Gly Pro His Val Tyr Gln 
        275                 280                 285             


Asp Leu Leu Leu Ser Leu Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro 
    290                 295                 300                 


Phe Ser Leu Gln Ser Gly Ile Leu Thr Leu Asn Pro Val Ala Gln Gly 
305                 310                 315                 320 


Gln Pro Ile Leu Thr Ser Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val 
                325                 330                 335     


Thr Met Ser Ser Phe Tyr Gln Asn Gln 
            340                 345 


<210>  18
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker polypeptide

<400>  18

Gly Gly Gly Ser 
1               


<210>  19
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker polypeptide

<400>  19

Gly Gly Gly Ser Gly Gly Gly 
1               5           


<210>  20
<211>  3
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker polypeptide

<400>  20

Gly Gly Gly 
1           


<210>  21
<211>  3
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker polypeptide

<400>  21

Gly Gly Ser 
1           


<210>  22
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker polypeptide

<400>  22

Gly Gly Ser Pro 
1               


<210>  23
<211>  251
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rgamma-CISC polypeptide

<400>  23

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        115                 120                 125             


Gly Ser Asn Thr Ser Lys Glu Asn Pro Phe Leu Phe Ala Leu Glu Ala 
    130                 135                 140                 


Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
145                 150                 155                 160 


Val Tyr Phe Trp Leu Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys 
                165                 170                 175     


Asn Leu Glu Asp Leu Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp 
            180                 185                 190         


Ser Gly Val Ser Lys Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser 
        195                 200                 205             


Glu Arg Leu Cys Leu Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu 
    210                 215                 220                 


Gly Glu Gly Pro Gly Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp 
225                 230                 235                 240 


Ala Pro Pro Cys Tyr Thr Leu Lys Pro Glu Thr 
                245                 250     


<210>  24
<211>  443
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Rbeta-CISC polypeptide

<400>  24

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        115                 120                 125             


Gly Lys Asp Thr Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser 
    130                 135                 140                 


Gly Ala Phe Gly Phe Ile Ile Leu Val Tyr Leu Leu Ile Asn Cys Arg 
145                 150                 155                 160 


Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn Thr Pro Asp 
                165                 170                 175     


Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly Gly Asp Val 
            180                 185                 190         


Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe Ser Pro Gly 
        195                 200                 205             


Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu Arg Asp Lys 
    210                 215                 220                 


Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu Pro Ala Ser 
225                 230                 235                 240 


Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn Gln Gly Tyr 
                245                 250                 255     


Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala Cys Gln Val 
            260                 265                 270         


Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp Glu Gly Val 
        275                 280                 285             


Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln Pro Leu Ser 
    290                 295                 300                 


Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp Asp Leu Leu 
305                 310                 315                 320 


Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro Ser Thr Ala 
                325                 330                 335     


Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro Ser Leu Gln 
            340                 345                 350         


Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly Pro Pro Thr 
        355                 360                 365             


Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro Glu Leu Val 
    370                 375                 380                 


Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro Arg Glu Gly 
385                 390                 395                 400 


Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu Phe Arg Ala 
                405                 410                 415     


Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu Ser Leu Gln 
            420                 425                 430         


Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
        435                 440             


<210>  25
<211>  358
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL2Ralpha-CISC polypeptide

<400>  25

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        115                 120                 125             


Glu Ile Asn Asn Ser Ser Gly Glu Met Asp Pro Ile Leu Leu Thr Ile 
    130                 135                 140                 


Ser Ile Leu Ser Phe Phe Ser Val Ala Leu Leu Val Ile Leu Ala Cys 
145                 150                 155                 160 


Val Leu Trp Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro 
                165                 170                 175     


Asp His Lys Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn 
            180                 185                 190         


Leu Asn Val Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His 
        195                 200                 205             


Arg Val Asp Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln 
    210                 215                 220                 


Asp Thr Phe Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln Arg Leu Gly 
225                 230                 235                 240 


Gly Asp Val Gln Ser Pro Asn Cys Pro Ser Glu Asp Val Val Ile Thr 
                245                 250                 255     


Pro Glu Ser Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu Ala Gly Asn 
            260                 265                 270         


Val Ser Ala Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg Ser Leu Asp 
        275                 280                 285             


Cys Arg Glu Ser Gly Lys Asn Gly Pro His Val Tyr Gln Asp Leu Leu 
    290                 295                 300                 


Leu Ser Leu Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro Phe Ser Leu 
305                 310                 315                 320 


Gln Ser Gly Ile Leu Thr Leu Asn Pro Val Ala Gln Gly Gln Pro Ile 
                325                 330                 335     


Leu Thr Ser Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val Thr Met Ser 
            340                 345                 350         


Ser Phe Tyr Gln Asn Gln 
        355             


<210>  26
<211>  358
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  IL7Ralpha-CISC polypeptide

<400>  26

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
        115                 120                 125             


Glu Ile Asn Asn Ser Ser Gly Glu Met Asp Pro Ile Leu Leu Thr Ile 
    130                 135                 140                 


Ser Ile Leu Ser Phe Phe Ser Val Ala Leu Leu Val Ile Leu Ala Cys 
145                 150                 155                 160 


Val Leu Trp Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro 
                165                 170                 175     


Asp His Lys Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn 
            180                 185                 190         


Leu Asn Val Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His 
        195                 200                 205             


Arg Val Asp Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln 
    210                 215                 220                 


Asp Thr Phe Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln Arg Leu Gly 
225                 230                 235                 240 


Gly Asp Val Gln Ser Pro Asn Cys Pro Ser Glu Asp Val Val Ile Thr 
                245                 250                 255     


Pro Glu Ser Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu Ala Gly Asn 
            260                 265                 270         


Val Ser Ala Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg Ser Leu Asp 
        275                 280                 285             


Cys Arg Glu Ser Gly Lys Asn Gly Pro His Val Tyr Gln Asp Leu Leu 
    290                 295                 300                 


Leu Ser Leu Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro Phe Ser Leu 
305                 310                 315                 320 


Gln Ser Gly Ile Leu Thr Leu Asn Pro Val Ala Gln Gly Gln Pro Ile 
                325                 330                 335     


Leu Thr Ser Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val Thr Met Ser 
            340                 345                 350         


Ser Phe Tyr Gln Asn Gln 
        355             


<210>  27
<211>  276
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  MPL-CISC polypeptide

<400>  27

Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      


His Ala Gln Ala Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
            20                  25                  30          


Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
        35                  40                  45              


Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys 
    50                  55                  60                  


Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
65                  70                  75                  80  


Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
                85                  90                  95      


Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
            100                 105                 110         


Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Gly Glu 
        115                 120                 125             


Glu Thr Ala Trp Ile Ser Leu Val Thr Ala Leu His Leu Val Leu Gly 
    130                 135                 140                 


Leu Ser Ala Val Leu Gly Leu Leu Leu Leu Arg Trp Gln Phe Pro Ala 
145                 150                 155                 160 


His Tyr Arg Arg Leu Arg His Ala Leu Trp Pro Ser Leu Pro Asp Leu 
                165                 170                 175     


His Arg Val Leu Gly Gln Tyr Leu Arg Asp Thr Ala Ala Leu Ser Pro 
            180                 185                 190         


Pro Lys Ala Thr Val Ser Asp Thr Cys Glu Glu Val Glu Pro Ser Leu 
        195                 200                 205             


Leu Glu Ile Leu Pro Lys Ser Ser Glu Arg Thr Pro Leu Pro Leu Cys 
    210                 215                 220                 


Ser Ser Gln Ala Gln Met Asp Tyr Arg Arg Leu Gln Pro Ser Cys Leu 
225                 230                 235                 240 


Gly Thr Met Pro Leu Ser Val Cys Pro Pro Met Ala Glu Ser Gly Ser 
                245                 250                 255     


Cys Cys Thr Thr His Ile Ala Asn His Ser Tyr Leu Pro Leu Ser Tyr 
            260                 265                 270         


Trp Gln Gln Pro 
        275     


<210>  28
<211>  9405
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CISC vector DNA

<400>  28
agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag       60

caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg      120

tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact      180

gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc      240

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct      300

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga      360

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg      420

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact      480

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa      540

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg      600

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat      660

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc      720

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag      780

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat      840

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac      900

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat      960

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg     1020

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat     1080

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat     1140

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt     1200

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca     1260

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat     1320

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag     1380

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat     1440

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa     1500

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat     1560

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt     1620

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt     1680

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga     1740

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta     1800

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     1860

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca     1920

aaattttatc gatcacgaga ctagcctcga gaagcttgat atcgaattcc cacggggttg     1980

gacgcgtagg aacagagaaa caggagaata tgggccaaac aggatatctg tggtaagcag     2040

ttcctgcccc ggctcagggc caagaacagt tggaacagca gaatatgggc caaacaggat     2100

atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg     2160

gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct     2220

gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg     2280

cgcttctgct ccccgagctc tatataagca gagctcgttt agtgaaccgt cagatcgcta     2340

gcaccggtgc cgccaccatg cctctgggcc tgctgtggct gggcctggcc ctgctgggcg     2400

ccctgcacgc ccaggccggc gtgcaggtgg agacaatctc cccaggcgac ggacgcacat     2460

tccctaagcg gggccagacc tgcgtggtgc actatacagg catgctggag gatggcaaga     2520

agtttgacag ctcccgggat agaaacaagc cattcaagtt tatgctgggc aagcaggaag     2580

tgatcagagg ctgggaggag ggcgtggccc agatgtctgt gggccagagg gccaagctga     2640

ccatcagccc agactacgcc tatggagcaa caggccaccc aggaatcatc ccacctcacg     2700

ccaccctggt gttcgatgtg gagctgctga agctgggcga gggatccaac acatcaaaag     2760

agaacccctt tctgttcgca ttggaggccg tagtcatatc tgttggatcc atgggactta     2820

ttatctccct gttgtgtgtg tacttctggc tggaacggac tatgcccagg atccccacgc     2880

tcaagaatct ggaagatctc gtcacagaat accatggtaa tttcagcgcc tggagcggag     2940

tctctaaggg tctggccgaa tccctccaac ccgattattc tgaacggttg tgcctcgtat     3000

ccgaaatacc accaaaaggc ggggctctgg gtgagggccc aggggcgagt ccgtgcaatc     3060

aacacagccc gtattgggcc cctccttgtt atacgttgaa gcccgaaact ggaagcggag     3120

ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgg     3180

cactgcccgt gaccgccctg ctgctgcctc tggccctgct gctgcacgca gcccggccta     3240

tcctgtggca cgagatgtgg cacgagggcc tggaggaggc cagcaggctg tattttggcg     3300

agcgcaacgt gaagggcatg ttcgaggtgc tggagcctct gcacgccatg atggagagag     3360

gcccacagac cctgaaggag acatccttta accaggccta tggacgggac ctgatggagg     3420

cacaggagtg gtgcagaaag tacatgaagt ctggcaatgt gaaggacctg ctgcaggcct     3480

gggatctgta ctatcacgtg tttcggagaa tctccaaggg caaagacacg attccgtggc     3540

ttgggcatct gctcgttggg ctgagtggtg cgtttggttt catcatcttg gtctatctct     3600

tgatcaattg cagaaataca ggcccttggc tgaaaaaagt gctcaagtgt aatacccccg     3660

acccaagcaa gttcttctcc cagctttctt cagagcatgg aggcgatgtg cagaaatggc     3720

tctcttcacc ttttccctcc tcaagcttct ccccgggagg gctggcgccc gagatttcac     3780

ctcttgaggt acttgaacga gacaaggtta cccaacttct ccttcaacag gataaggtac     3840

ccgaacctgc gagccttagc tccaaccact ctcttacgag ctgcttcacc aatcagggat     3900

acttcttttt ccaccttccc gatgcgctgg aaatcgaagc ttgtcaagtt tactttacct     3960

atgatccata tagcgaggaa gatcccgacg aaggagtcgc cggtgcgccc acgggttcct     4020

caccccaacc tctccagcct ctctcaggag aagatgatgc ttattgcact tttcccagta     4080

gagacgatct cctcctcttt tctccatctc ttttgggggg accttccccc ccttctacgg     4140

cacctggcgg gtctggtgct ggcgaggagc ggatgccgcc gtccctccag gagcgagtac     4200

cacgagattg ggatccccag ccacttggac cccccacccc cggcgtacct gaccttgtcg     4260

attttcaacc tccccctgaa ttggtgctgc gagaggctgg ggaggaagtt ccggacgctg     4320

ggccgaggga gggcgtgtcc tttccatgga gtaggcctcc aggtcaaggc gagtttaggg     4380

ctctcaacgc gcggctgccg ttgaatacag acgcttatct ctcactgcag gaactgcaag     4440

gtcaggaccc aacacatctt gtaggatctg gtgctactaa tttttctctt ttgaagcaag     4500

ctggagatgt tgaagagaac cctggtccag tgagcaaggg cgaggagctg ttcaccgggg     4560

tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg     4620

gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg     4680

gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct     4740

tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag     4800

gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg     4860

aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca     4920

aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct     4980

atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca     5040

tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg     5100

gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc     5160

ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc     5220

tcggcatgga cgagctgtac aagtaaacta gtgtcgacaa tcaacctctg gattacaaaa     5280

tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg     5340

ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct     5400

tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg     5460

gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct     5520

gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg     5580

ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg     5640

tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc     5700

tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc     5760

gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc     5820

ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat     5880

gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg     5940

gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt     6000

agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca     6060

ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa     6120

ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg     6180

tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa     6240

cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa     6300

taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta     6360

tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc     6420

catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta     6480

ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcgtcga gacgtaccca     6540

attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg     6600

actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca     6660

gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga     6720

atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta     6780

cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc     6840

cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt     6900

tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg     6960

gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca     7020

cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct     7080

attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga     7140

tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccaggtgg     7200

cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa     7260

tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa     7320

gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct     7380

tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg     7440

tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg     7500

ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt     7560

atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga     7620

cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga     7680

attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac     7740

gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg     7800

ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac     7860

gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct     7920

agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct     7980

gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg     8040

gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat     8100

ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg     8160

tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat     8220

tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct     8280

catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa     8340

gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa     8400

aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc     8460

gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta     8520

gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct     8580

gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg     8640

atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag     8700

cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc     8760

cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg     8820

agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt     8880

tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg     8940

gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca     9000

catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg     9060

agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc     9120

ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag     9180

ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag     9240

ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg     9300

tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa     9360

gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgca                     9405


<210>  29
<211>  10053
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CISC vector DNA

<400>  29
agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag       60

caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg      120

tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact      180

gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc      240

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct      300

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga      360

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg      420

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact      480

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa      540

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg      600

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat      660

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc      720

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag      780

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat      840

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac      900

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat      960

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg     1020

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat     1080

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat     1140

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt     1200

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca     1260

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat     1320

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag     1380

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat     1440

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa     1500

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat     1560

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt     1620

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt     1680

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga     1740

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta     1800

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     1860

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca     1920

aaattttatc gatcacgaga ctagcctcga gaagcttgat atcgaattcc cacggggttg     1980

gacgcgtagg aacagagaaa caggagaata tgggccaaac aggatatctg tggtaagcag     2040

ttcctgcccc ggctcagggc caagaacagt tggaacagca gaatatgggc caaacaggat     2100

atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg     2160

gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct     2220

gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg     2280

cgcttctgct ccccgagctc tatataagca gagctcgttt agtgaaccgt cagatcgcta     2340

gcaccggtgc cgccaccatg cctctgggcc tgctgtggct gggcctggcc ctgctgggcg     2400

ccctgcacgc ccaggccggc gtgcaggtgg agacaatctc cccaggcgac ggacgcacat     2460

tccctaagcg gggccagacc tgcgtggtgc actatacagg catgctggag gatggcaaga     2520

agtttgacag ctcccgggat agaaacaagc cattcaagtt tatgctgggc aagcaggaag     2580

tgatcagagg ctgggaggag ggcgtggccc agatgtctgt gggccagagg gccaagctga     2640

ccatcagccc agactacgcc tatggagcaa caggccaccc aggaatcatc ccacctcacg     2700

ccaccctggt gttcgatgtg gagctgctga agctgggcga gggcggtagt cagaaccttg     2760

tgataccatg ggccccagaa aatctcacac ttcataaact ttccgaatca caactcgaac     2820

tcaactggaa taaccggttc ctgaatcact gtcttgaaca cctggtacaa tatcggaccg     2880

actgggatca ctcatggaca gaacaatctg tggactatag gcacaaattc tcactcccaa     2940

gcgtagacgg ccaaaaaaga tacacttttc gcgtacgatc ccgctttaat cctctctgcg     3000

gctctgctca gcactggagt gaatggtccc atcccattca ttggggatcc aacacatcaa     3060

aagagaaccc ctttctgttc gcattggagg ccgtagtcat atctgttgga tccatgggac     3120

ttattatctc cctgttgtgt gtgtacttct ggctggaacg gactatgccc aggatcccca     3180

cgctcaagaa tctggaagat ctcgtcacag aataccatgg taatttcagc gcctggagcg     3240

gagtctctaa gggtctggcc gaatccctcc aacccgatta ttctgaacgg ttgtgcctcg     3300

tatccgaaat accaccaaaa ggcggggctc tgggtgaggg cccaggggcg agtccgtgca     3360

atcaacacag cccgtattgg gcccctcctt gttatacgtt gaagcccgaa actggaagcg     3420

gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac cctggaccta     3480

tggcactgcc cgtgaccgcc ctgctgctgc ctctggccct gctgctgcac gcagcccggc     3540

ctatcctgtg gcacgagatg tggcacgagg gcctggagga ggccagcagg ctgtattttg     3600

gcgagcgcaa cgtgaagggc atgttcgagg tgctggagcc tctgcacgcc atgatggaga     3660

gaggcccaca gaccctgaag gagacatcct ttaaccaggc ctatggacgg gacctgatgg     3720

aggcacagga gtggtgcaga aagtacatga agtctggcaa tgtgaaggac ctgctgcagg     3780

cctgggatct gtactatcac gtgtttcgga gaatctccaa gggaggttca aaaccttttg     3840

agaaccttag actgatggcg cccatctctc tgcaggtagt tcacgttgag acccatagat     3900

gcaatataag ctgggaaatc tcacaagcca gccattactt tgaacggcat ttggaattcg     3960

aggcccgaac actttccccc ggtcatacgt gggaagaagc tcctctcttg acgctgaagc     4020

agaagcagga gtggatttgt ctggagactt tgactcctga tactcagtat gagttccaag     4080

ttcgggtgaa accactccaa ggcgagttca cgacgtggtc tccgtggagt caaccgttgg     4140

cgttccgcac gaagcccgct gcccttggca aagacacgat tccgtggctt gggcatctgc     4200

tcgttgggct gagtggtgcg tttggtttca tcatcttggt ctatctcttg atcaattgca     4260

gaaatacagg cccttggctg aaaaaagtgc tcaagtgtaa tacccccgac ccaagcaagt     4320

tcttctccca gctttcttca gagcatggag gcgatgtgca gaaatggctc tcttcacctt     4380

ttccctcctc aagcttctcc ccgggagggc tggcgcccga gatttcacct cttgaggtac     4440

ttgaacgaga caaggttacc caacttctcc ttcaacagga taaggtaccc gaacctgcga     4500

gccttagctc caaccactct cttacgagct gcttcaccaa tcagggatac ttctttttcc     4560

accttcccga tgcgctggaa atcgaagctt gtcaagttta ctttacctat gatccatata     4620

gcgaggaaga tcccgacgaa ggagtcgccg gtgcgcccac gggttcctca ccccaacctc     4680

tccagcctct ctcaggagaa gatgatgctt attgcacttt tcccagtaga gacgatctcc     4740

tcctcttttc tccatctctt ttggggggac cttccccccc ttctacggca cctggcgggt     4800

ctggtgctgg cgaggagcgg atgccgccgt ccctccagga gcgagtacca cgagattggg     4860

atccccagcc acttggaccc cccacccccg gcgtacctga ccttgtcgat tttcaacctc     4920

cccctgaatt ggtgctgcga gaggctgggg aggaagttcc ggacgctggg ccgagggagg     4980

gcgtgtcctt tccatggagt aggcctccag gtcaaggcga gtttagggct ctcaacgcgc     5040

ggctgccgtt gaatacagac gcttatctct cactgcagga actgcaaggt caggacccaa     5100

cacatcttgt aggatctggt gctactaatt tttctctttt gaagcaagct ggagatgttg     5160

aagagaaccc tggtccagtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc     5220

tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg     5280

gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg     5340

tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc     5400

ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg     5460

agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg     5520

agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca     5580

acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg     5640

acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca     5700

gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc     5760

tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc     5820

gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg     5880

agctgtacaa gtaaactagt gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat     5940

tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc     6000

ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct     6060

ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca     6120

ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt     6180

ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg     6240

cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga     6300

agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt     6360

ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc     6420

cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt     6480

gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc     6540

agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc     6600

ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg     6660

agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc     6720

ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct     6780

cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat     6840

tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc     6900

agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt     6960

ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct     7020

ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta     7080

atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag     7140

tgaggaggct tttttggagg cctaggcttt tgcgtcgaga cgtacccaat tcgccctata     7200

gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc     7260

ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata     7320

gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc     7380

gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga     7440

ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg     7500

ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat     7560

ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg     7620

ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata     7680

gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt     7740

tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat     7800

ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccaggtggca cttttcgggg     7860

aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct     7920

catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat     7980

tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc     8040

tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg     8100

ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg     8160

ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga     8220

cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta     8280

ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc     8340

tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc     8400

gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg     8460

ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc     8520

aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca     8580

acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct     8640

tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat     8700

cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg     8760

gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat     8820

taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact     8880

tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat     8940

cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc     9000

ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct     9060

accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg     9120

cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca     9180

cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc     9240

tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga     9300

taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac     9360

gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga     9420

agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag     9480

ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg     9540

acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag     9600

caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc     9660

tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc     9720

tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc     9780

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag     9840

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca     9900

ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag     9960

cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa    10020

ccctcactaa agggaacaaa agctggagct gca                                 10053


<210>  30
<211>  10035
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  CISC vector DNA

<400>  30
agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag       60

caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg      120

tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact      180

gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc      240

tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct      300

taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga      360

ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg      420

cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact      480

cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa      540

attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg      600

gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat      660

aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc      720

ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag      780

acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat      840

caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac      900

aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat      960

gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg     1020

agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat     1080

aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcctcaat     1140

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt     1200

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca     1260

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat     1320

ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag     1380

taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat     1440

taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa     1500

gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat     1560

aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt     1620

aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt     1680

atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga     1740

agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta     1800

tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     1860

agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca     1920

aaattttatc gatcacgaga ctagcctcga gaagcttgat atcgaattcc cacggggttg     1980

gacgcgtagg aacagagaaa caggagaata tgggccaaac aggatatctg tggtaagcag     2040

ttcctgcccc ggctcagggc caagaacagt tggaacagca gaatatgggc caaacaggat     2100

atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg     2160

gtcccgccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct     2220

gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg     2280

cgcttctgct ccccgagctc tatataagca gagctcgttt agtgaaccgt cagatcgcta     2340

gcaccggtgc cgccaccatg cctctgggcc tgctgtggct gggcctggcc ctgctgggcg     2400

ccctgcacgc ccaggccggc gtgcaggtgg agacaatctc cccaggcgac ggacgcacat     2460

tccctaagcg gggccagacc tgcgtggtgc actatacagg catgctggag gatggcaaga     2520

agtttgacag ctcccgggat agaaacaagc cattcaagtt tatgctgggc aagcaggaag     2580

tgatcagagg ctgggaggag ggcgtggccc agatgtctgt gggccagagg gccaagctga     2640

ccatcagccc agactacgcc tatggagcaa caggccaccc aggaatcatc ccacctcacg     2700

ccaccctggt gttcgatgtg gagctgctga agctgggcga gcaaaacttg gtgattcctt     2760

gggccccaga aaatctcacg cttcacaagt tgtccgaatc ccagctcgag ctcaactgga     2820

ataatagatt tcttaatcat tgtttggaac acctggttca atatagaacg gattgggacc     2880

actcatggac cgagcagtca gttgactacc gccacaaatt ttcacttccc agcgtagatg     2940

ggcagaagag gtacacattt agggtcagat ccaggtttaa tcctctgtgt ggttctgctc     3000

aacactggtc tgagtggagc catccgatcc actggggctc aaatacctct aaagaaaatc     3060

cgttcctctt tgcgctcgaa gccgttgtta tcagcgtcgg aagcatggga cttatcattt     3120

cccttctctg cgtgtacttc tggctggagc ggacgatgcc gcggattccg acgctcaaaa     3180

acctggagga ccttgtaaca gaatatcacg gtaatttctc cgcttggagt ggcgtatcaa     3240

aggggcttgc tgagtccctt caaccggatt actctgagcg cctctgcttg gtgtccgaga     3300

tacctcccaa aggaggtgca cttggggagg ggccaggcgc gtccccttgc aatcagcata     3360

gtccgtattg ggcgcccccc tgttataccc tcaaaccgga aacgggaagc ggagctacta     3420

acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct atggcactgc     3480

ccgtgaccgc cctgctgctg cctctggccc tgctgctgca cgcagcccgg cctatcctgt     3540

ggcacgagat gtggcacgag ggcctggagg aggccagcag gctgtatttt ggcgagcgca     3600

acgtgaaggg catgttcgag gtgctggagc ctctgcacgc catgatggag agaggcccac     3660

agaccctgaa ggagacatcc tttaaccagg cctatggacg ggacctgatg gaggcacagg     3720

agtggtgcag aaagtacatg aagtctggca atgtgaagga cctgctgcag gcctgggatc     3780

tgtactatca cgtgtttcgg agaatctcca agaaaccttt tgagaacctt agactgatgg     3840

cgcccatctc tctgcaggta gttcacgttg agacccatag atgcaatata agctgggaaa     3900

tctcacaagc cagccattac tttgaacggc atttggaatt cgaggcccga acactttccc     3960

ccggtcatac gtgggaagaa gctcctctct tgacgctgaa gcagaagcag gagtggattt     4020

gtctggagac tttgactcct gatactcagt atgagttcca agttcgggtg aaaccactcc     4080

aaggcgagtt cacgacgtgg tctccgtgga gtcaaccgtt ggcgttccgc acgaagcccg     4140

ctgcccttgg caaagacacg attccgtggc ttgggcatct gctcgttggg ctgagtggtg     4200

cgtttggttt catcatcttg gtctatctct tgatcaattg cagaaataca ggcccttggc     4260

tgaaaaaagt gctcaagtgt aatacccccg acccaagcaa gttcttctcc cagctttctt     4320

cagagcatgg aggcgatgtg cagaaatggc tctcttcacc ttttccctcc tcaagcttct     4380

ccccgggagg gctggcgccc gagatttcac ctcttgaggt acttgaacga gacaaggtta     4440

cccaacttct ccttcaacag gataaggtac ccgaacctgc gagccttagc tccaaccact     4500

ctcttacgag ctgcttcacc aatcagggat acttcttttt ccaccttccc gatgcgctgg     4560

aaatcgaagc ttgtcaagtt tactttacct atgatccata tagcgaggaa gatcccgacg     4620

aaggagtcgc cggtgcgccc acgggttcct caccccaacc tctccagcct ctctcaggag     4680

aagatgatgc ttattgcact tttcccagta gagacgatct cctcctcttt tctccatctc     4740

ttttgggggg accttccccc ccttctacgg cacctggcgg gtctggtgct ggcgaggagc     4800

ggatgccgcc gtccctccag gagcgagtac cacgagattg ggatccccag ccacttggac     4860

cccccacccc cggcgtacct gaccttgtcg attttcaacc tccccctgaa ttggtgctgc     4920

gagaggctgg ggaggaagtt ccggacgctg ggccgaggga gggcgtgtcc tttccatgga     4980

gtaggcctcc aggtcaaggc gagtttaggg ctctcaacgc gcggctgccg ttgaatacag     5040

acgcttatct ctcactgcag gaactgcaag gtcaggaccc aacacatctt gtaggatctg     5100

gtgctactaa tttttctctt ttgaagcaag ctggagatgt tgaagagaac cctggtccag     5160

tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg     5220

acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca     5280

agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg     5340

tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc     5400

acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca     5460

aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga     5520

accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc     5580

tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca     5640

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc     5700

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc     5760

tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc     5820

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaacta     5880

gtgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact     5940

atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg     6000

cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg     6060

aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa     6120

cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc     6180

ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg     6240

ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat     6300

ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt     6360

cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc     6420

cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg     6480

gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca     6540

ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct     6600

gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg     6660

ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt     6720

gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt     6780

gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc     6840

aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa     6900

ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg     6960

tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta     7020

actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca     7080

gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga     7140

ggcctaggct tttgcgtcga gacgtaccca attcgcccta tagtgagtcg tattacgcgc     7200

gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta     7260

atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg     7320

atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg     7380

gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg     7440

ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc     7500

cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc     7560

tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga     7620

cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa     7680

ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga     7740

tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca     7800

aaatattaac gtttacaatt tcccaggtgg cacttttcgg ggaaatgtgc gcggaacccc     7860

tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg     7920

ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc     7980

ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt     8040

gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct     8100

caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac     8160

ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact     8220

cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa     8280

gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga     8340

taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt     8400

tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga     8460

agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg     8520

caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat     8580

ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat     8640

tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc     8700

agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga     8760

tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc     8820

agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag     8880

gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc     8940

gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt     9000

tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt     9060

gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat     9120

accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc     9180

accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa     9240

gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg     9300

ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag     9360

atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag     9420

gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa     9480

cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt     9540

gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg     9600

gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc     9660

tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac     9720

cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct     9780

ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc     9840

gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt     9900

acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac     9960

aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca    10020

aaagctggag ctgca                                                     10035


<210>  31
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic polypeptide

<220>
<221>  misc_feature
<223>  Linker/spacer polypeptide

<400>  31

Pro Ala Ala Leu 
1               


<210>  32
<211>  267
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Naked FRB domain nucleic acid sequence

<400>  32
gagatgtggc atgagggtct ggaagaagcg tctcgactgt actttggtga gcgcaatgtg       60

aagggcatgt ttgaagtcct cgaacccctt catgccatga tggaacgcgg accccagacc      120

ttgaaggaga caagttttaa ccaagcttac ggaagagacc tgatggaagc ccaggaatgg      180

tgcaggaaat acatgaaaag cgggaatgtg aaggacttga cccaagcgtg ggacctgtac      240

tatcatgtct ttaggcgcat tagtaag                                          267


<210>  33
<211>  353
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  misc_feature
<223>  MND promoter

<400>  33
gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc       60

cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt      120

aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc      180

tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc      240

ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc      300

tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatcgct agc             353


<210>  34
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  DICS HDR FP

<400>  34
cggcgacgtg gaagagaatc                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  DISC HDR RP

<400>  35
ggctgtggtt cagcctgact                                                   20


<210>  36
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  DISC HDR probe (FAM)

<400>  36
aggctctccc cgacctccc                                                    19


<210>  37
<211>  2880
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Coding sequences for 3186 
       (Signal-FKBP-IL2Rg-P2A-signal-FRB-IL2Rb-P2A-FRB-P2A-GFP-FOXP3 
       (knock-in)

<400>  37
atgccactgg gactgctgtg gcttggactg gcccttcttg gagcactgca tgctcaggct       60

ggcgtgcagg tcgagacaat tagtcctggc gacggccgga cctttcctaa gcgaggacag      120

acatgcgtgg tgcactacac cggcatgctg gaagatggca agaagttcga cagcagccgg      180

gacagaaaca agcctttcaa gttcatgctg ggcaagcaag aagtgatcag aggctgggaa      240

gagggcgtcg cccagatgtc tgttggacag agagccaagc tgacaatcag ccccgattac      300

gcctatggcg ccacaggaca ccctgggatc attcctccac atgccacact ggtgttcgat      360

gtggaactgc tgaagctcgg cgaaggcagc aataccagca aagagaaccc cttcctgttc      420

gccctggaag ccgtggttat cagcgtggga tctatgggcc tgatcatctc cctgctgtgc      480

gtgtacttct ggctggaacg gaccatgcct cggatcccca cactgaagaa tctggaagat      540

ctggtcaccg agtaccacgg caatttcagc gcttggagtg gcgtgtccaa aggcctggct      600

gaaagcctgc agcctgacta ctctgagaga ctgtgcctgg tgtctgagat ccctcctaaa      660

ggcggcgctc tcggagaagg accaggcgct tctccatgca atcagcacag cccttattgg      720

gcccctcctt gctacaccct gaagcctgaa actggaagcg gagctactaa ctttagcctg      780

ctgaagcagg ctggagacgt ggaggagaac cctggaccta tggctctgcc agtgacagct      840

ctgctgcttc ctctggctct gttgctgcat gccgccagac ctattctgtg gcacgagatg      900

tggcatgaag gcctggaaga ggcctccaga ctgtacttcg gcgagagaaa cgtgaagggc      960

atgttcgagg tgctggaacc cctgcatgcc atgatggaaa gaggccctca gacactgaaa     1020

gagacaagct tcaaccaggc ctacggccgg gatctgatgg aagcccaaga gtggtgccgg     1080

aagtacatga agtccggcaa tgtgaaggac ctcctgcagg catgggacct gtactaccac     1140

gtgttccggc ggatctctaa gggcaaagac acaatccctt ggctgggcca tctgctcgtt     1200

ggactgtctg gcgccttcgg cttcatcatc ctggtgtacc tgctgatcaa ctgtcggaac     1260

acaggcccat ggctgaagaa agtgctgaag tgcaacaccc ctgatccgag caagttcttt     1320

agccagctgt ccagcgagca cggcggagat gttcagaagt ggctgagcag cccatttcct     1380

agcagcagct ttagccctgg cggactggct cctgagatca gcccactgga agtgctggaa     1440

agggacaaag tgacccagct gctcctgcaa caggacaagg tgccagaacc tgccagcctg     1500

tctctgaaca ccgatgccta tctgtccctg caagagctgc aaggatccgg cgccaccaac     1560

tttagtctgc tcaagcaagc cggggacgtc gaggaaaatc ctgggccaga aatgtggcac     1620

gaaggactcg aggaagccag tcggctgtat tttggcgagc ggaatgtgaa agggatgttt     1680

gaagtgctcg agcctctcca cgctatgatg gaacggggac cccagactct caaagaaacc     1740

agctttaatc aggcttacgg acgcgacctc atggaagctc aagaatggtg tagaaagtat     1800

atgaagagtg gcaacgtgaa agatctgctg caagcctggg atctctatta tcacgtgttc     1860

agacgcatca gcaaaggcag cggcgccaca aatttctccc tgctgaaaca ggccggcgac     1920

gtggaagaga atcccggacc tatgcctaat cctcggcctt ccaaaggcga ggaactgttt     1980

acaggcgtgg tgcccatcct ggtggaactg gacggggatg tgaacggcca caagtttagc     2040

gttagcggcg aaggcgaagg ggatgccaca tacggaaagc tgaccctgaa gttcatctgc     2100

accaccggca agctgcctgt gccttggcct acactggtca ccacactgac atacggcgtg     2160

cagtgcttca gcagataccc cgaccatatg aagcagcacg acttcttcaa gagcgccatg     2220

cctgagggct acgtgcaaga gcggaccatc ttctttaagg acgacggcaa ctacaagacc     2280

agggccgaag tgaagttcga gggcgacacc ctggtcaacc ggatcgagct gaagggcatc     2340

gacttcaaag aggacggcaa catcctgggc cacaagctcg agtacaacta caacagccac     2400

aacgtgtaca tcatggccga caagcagaaa aacggcatca aagtgaactt caagatccgg     2460

cacaacatcg aggacggctc tgtgcagctg gccgatcact accagcagaa cacacccatc     2520

ggagatggcc ctgtgctgct gcccgataac cactacctga gcacacagag cgccctgagc     2580

aaggacccca acgagaagag ggatcacatg gtgctgctgg aattcgtgac cgccgctggc     2640

atcacactcg gcatggatga gctgtacaag atgcccaatc ctagacctgg caagcccagc     2700

gctccttctc ttgctcttgg accttctcct ggtgcctcgc ccagctggag ggctgcaccc     2760

aaagcctcag acctgctggg ggcccggggc ccagggggaa ccttccaggg ccgagatctt     2820

cgaggcgggg cccatgcctc ctcttcttcc ttgaacccca tgccaccatc gcagctgcag     2880


<210>  38
<211>  2742
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Coding sequences for 3187 
       (Signal-FKBP-IL2Rg-P2A-signal-FRB-IL2Rb-P2A-FRB-P2A-HA-FOXP3 
       (knock-in)

<400>  38
atgccactgg gactgctgtg gcttggactg gcccttcttg gagcactgca tgctcaggct       60

ggcgtgcagg tcgagacaat tagtcctggc gacggccgga cctttcctaa gcgaggacag      120

acatgcgtgg tgcactacac cggcatgctg gaagatggca agaagttcga cagcagccgg      180

gacagaaaca agcctttcaa gttcatgctg ggcaagcaag aagtgatcag aggctgggaa      240

gagggcgtcg cccagatgtc tgttggacag agagccaagc tgacaatcag ccccgattac      300

gcctatggcg ccacaggaca ccctgggatc attcctccac atgccacact ggtgttcgat      360

gtggaactgc tgaagctcgg cgaaggcagc aataccagca aagagaaccc cttcctgttc      420

gccctggaag ccgtggttat cagcgtggga tctatgggcc tgatcatctc cctgctgtgc      480

gtgtacttct ggctggaacg gaccatgcct cggatcccca cactgaagaa tctggaagat      540

ctggtcaccg agtaccacgg caatttcagc gcttggagtg gcgtgtccaa aggcctggct      600

gaaagcctgc agcctgacta ctctgagaga ctgtgcctgg tgtctgagat ccctcctaaa      660

ggcggcgctc tcggagaagg accaggcgct tctccatgca atcagcacag cccttattgg      720

gcccctcctt gctacaccct gaagcctgaa actggaagcg gagctactaa ctttagcctg      780

ctgaagcagg ctggagacgt ggaggagaac cctggaccta tggctctgcc agtgacagct      840

ctgctgcttc ctctggctct gttgctgcat gccgccagac ctattctgtg gcacgagatg      900

tggcatgaag gcctggaaga ggcctccaga ctgtacttcg gcgagagaaa cgtgaagggc      960

atgttcgagg tgctggaacc cctgcatgcc atgatggaaa gaggccctca gacactgaaa     1020

gagacaagct tcaaccaggc ctacggccgg gatctgatgg aagcccaaga gtggtgccgg     1080

aagtacatga agtccggcaa tgtgaaggac ctcctgcagg catgggacct gtactaccac     1140

gtgttccggc ggatctctaa gggcaaagac acaatccctt ggctgggcca tctgctcgtt     1200

ggactgtctg gcgccttcgg cttcatcatc ctggtgtacc tgctgatcaa ctgtcggaac     1260

acaggcccat ggctgaagaa agtgctgaag tgcaacaccc ctgatccgag caagttcttt     1320

agccagctgt ccagcgagca cggcggagat gttcagaagt ggctgagcag cccatttcct     1380

agcagcagct ttagccctgg cggactggct cctgagatca gcccactgga agtgctggaa     1440

agggacaaag tgacccagct gctcctgcaa caggacaagg tgccagaacc tgccagcctg     1500

tctagcaatc acagcctgac cagctgcttt accaaccagg gctacttctt cttccatctg     1560

cctgacgctc tggaaatcga ggcctgccag gtgtacttca cctacgatcc ctacagcgaa     1620

gaggaccccg atgaaggtgt tgccggtgct cctaccggaa gctctcctca acctctgcaa     1680

ccactgagcg gcgaggatga cgcctactgc acattcccca gcagagatga cctgctgctg     1740

ttcagccctt ctctgctcgg cggaccttct ccaccatcta cagctccagg tggaagcgga     1800

gccggcgagg aaagaatgcc tccaagcctg caagagcggg tgcccagaga ttgggatcct     1860

caaccactgg gccctccaac acctggcgtg ccagatctcg tggatttcca gcctcctcca     1920

gagctggtgc tgagagaagc tggcgaagaa gtgccagacg ctggccctag agagggcgtt     1980

agctttcctt ggagcagacc tcctggacag ggcgagttca gagccctgaa tgctagactg     2040

cccctgaaca ccgatgccta tctgtccctg caagagctgc aaggacaaga ccccacacac     2100

ctggttggat ccggcgccac caactttagt ctgctcaagc aagccgggga cgtcgaggaa     2160

aatcctgggc cagaaatgtg gcacgaagga ctcgaggaag ccagtcggct gtattttggc     2220

gagcggaatg tgaaagggat gtttgaagtg ctcgagcctc tccacgctat gatggaacgg     2280

ggaccccaga ctctcaaaga aaccagcttt aatcaggctt acggacgcga cctcatggaa     2340

gctcaagaat ggtgtagaaa gtatatgaag agtggcaacg tgaaagatct gctgcaagcc     2400

tgggatctct attatcacgt gttcagacgc atcagcaaag gcagcggcgc cacaaatttc     2460

tccctgctga aacaggccgg cgacgtggaa gagaatcccg gacctatgta tccatacgat     2520

gtcccagatt atgcgcccaa tcctagacct ggcaagccca gcgctccttc tcttgctctt     2580

ggaccttctc ctggtgcctc gcccagctgg agggctgcac ccaaagcctc agacctgctg     2640

ggggcccggg gcccaggggg aaccttccag ggccgagatc ttcgaggcgg ggcccatgcc     2700

tcctcttctt ccttgaaccc catgccacca tcgcagctgc ag                        2742


<210>  39
<211>  2715
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  Coding sequences for 3195 
       (Signal-FKBP-IL2Rg-P2A-signal-FRB-IL2Rb-P2A-FRB-P2A-FOXP3 
       (knock-in)

<400>  39
atgccactgg gactgctgtg gcttggactg gcccttcttg gagcactgca tgctcaggct       60

ggcgtgcagg tcgagacaat tagtcctggc gacggccgga cctttcctaa gcgaggacag      120

acatgcgtgg tgcactacac cggcatgctg gaagatggca agaagttcga cagcagccgg      180

gacagaaaca agcctttcaa gttcatgctg ggcaagcaag aagtgatcag aggctgggaa      240

gagggcgtcg cccagatgtc tgttggacag agagccaagc tgacaatcag ccccgattac      300

gcctatggcg ccacaggaca ccctgggatc attcctccac atgccacact ggtgttcgat      360

gtggaactgc tgaagctcgg cgaaggcagc aataccagca aagagaaccc cttcctgttc      420

gccctggaag ccgtggttat cagcgtggga tctatgggcc tgatcatctc cctgctgtgc      480

gtgtacttct ggctggaacg gaccatgcct cggatcccca cactgaagaa tctggaagat      540

ctggtcaccg agtaccacgg caatttcagc gcttggagtg gcgtgtccaa aggcctggct      600

gaaagcctgc agcctgacta ctctgagaga ctgtgcctgg tgtctgagat ccctcctaaa      660

ggcggcgctc tcggagaagg accaggcgct tctccatgca atcagcacag cccttattgg      720

gcccctcctt gctacaccct gaagcctgaa actggaagcg gagctactaa ctttagcctg      780

ctgaagcagg ctggagacgt ggaggagaac cctggaccta tggctctgcc agtgacagct      840

ctgctgcttc ctctggctct gttgctgcat gccgccagac ctattctgtg gcacgagatg      900

tggcatgaag gcctggaaga ggcctccaga ctgtacttcg gcgagagaaa cgtgaagggc      960

atgttcgagg tgctggaacc cctgcatgcc atgatggaaa gaggccctca gacactgaaa     1020

gagacaagct tcaaccaggc ctacggccgg gatctgatgg aagcccaaga gtggtgccgg     1080

aagtacatga agtccggcaa tgtgaaggac ctcctgcagg catgggacct gtactaccac     1140

gtgttccggc ggatctctaa gggcaaagac acaatccctt ggctgggcca tctgctcgtt     1200

ggactgtctg gcgccttcgg cttcatcatc ctggtgtacc tgctgatcaa ctgtcggaac     1260

acaggcccat ggctgaagaa agtgctgaag tgcaacaccc ctgatccgag caagttcttt     1320

agccagctgt ccagcgagca cggcggagat gttcagaagt ggctgagcag cccatttcct     1380

agcagcagct ttagccctgg cggactggct cctgagatca gcccactgga agtgctggaa     1440

agggacaaag tgacccagct gctcctgcaa caggacaagg tgccagaacc tgccagcctg     1500

tctagcaatc acagcctgac cagctgcttt accaaccagg gctacttctt cttccatctg     1560

cctgacgctc tggaaatcga ggcctgccag gtgtacttca cctacgatcc ctacagcgaa     1620

gaggaccccg atgaaggtgt tgccggtgct cctaccggaa gctctcctca acctctgcaa     1680

ccactgagcg gcgaggatga cgcctactgc acattcccca gcagagatga cctgctgctg     1740

ttcagccctt ctctgctcgg cggaccttct ccaccatcta cagctccagg tggaagcgga     1800

gccggcgagg aaagaatgcc tccaagcctg caagagcggg tgcccagaga ttgggatcct     1860

caaccactgg gccctccaac acctggcgtg ccagatctcg tggatttcca gcctcctcca     1920

gagctggtgc tgagagaagc tggcgaagaa gtgccagacg ctggccctag agagggcgtt     1980

agctttcctt ggagcagacc tcctggacag ggcgagttca gagccctgaa tgctagactg     2040

cccctgaaca ccgatgccta tctgtccctg caagagctgc aaggacaaga ccccacacac     2100

ctggttggat ccggcgccac caactttagt ctgctcaagc aagccgggga cgtcgaggaa     2160

aatcctgggc cagaaatgtg gcacgaagga ctcgaggaag ccagtcggct gtattttggc     2220

gagcggaatg tgaaagggat gtttgaagtg ctcgagcctc tccacgctat gatggaacgg     2280

ggaccccaga ctctcaaaga aaccagcttt aatcaggctt acggacgcga cctcatggaa     2340

gctcaagaat ggtgtagaaa gtatatgaag agtggcaacg tgaaagatct gctgcaagcc     2400

tgggatctct attatcacgt gttcagacgc atcagcaaag gcagcggcgc cacaaatttc     2460

tccctgctga aacaggccgg cgacgtggaa gagaatcccg gacctatgcc caatcctaga     2520

cctggcaagc ccagcgctcc ttctcttgct cttggacctt ctcctggtgc ctcgcccagc     2580

tggagggctg cacccaaagc ctcagacctg ctgggggccc ggggcccagg gggaaccttc     2640

cagggccgag atcttcgagg cggggcccat gcctcctctt cttccttgaa ccccatgcca     2700

ccatcgcagc tgcag                                                      2715


<210>  40
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  T1 spacer targeting human FOXP3

<400>  40
ttccagggcc gagatcttcg                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  T3 spacer targeting human FOXP3

<400>  41
cgcctcgaag atctcggccc                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  T4 spacer targeting human FOXP3

<400>  42
tcgaagatct cggccctgga                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  T7 spacer targeting human FOXP3

<400>  43
ggccctggaa ggttccccct                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  T9 spacer targeting human FOXP3

<400>  44
tccagctggg cgaggctcct                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  T18 spacer targeting human FOXP3

<400>  45
tcagacctgc tgggggcccg                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  R1 spacer targeting human FOXP3

<400>  46
gagccccgcc tcgaagatct                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  P1 spacer targeting human AAVS1

<400>  47
attcccaggg ccggttaatg                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  P3 spacer targeting human AAVS1

<400>  48
gtcccctcca ccccacagtg                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  P4 spacer targeting human AAVS1

<400>  49
accccacagt ggggccacta                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  N1 spacer targeting human AAVS1

<400>  50
cctctaaggt ttgcttacga                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  N2 spacer targeting human AAVS1

<400>  51
tataaggtgg tcccagctcg                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  N3 spacer targeting human AAVS1

<400>  52
ccatcgtaag caaaccttag                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  mT20 spacer target murine FOXP3

<400>  53
gactcctggg gatgggccaa                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  mT22 spacer target murine FOXP3

<400>  54
ttggcccttg gcccatcccc                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  mT23 spacer target murine FOXP3

<400>  55
ccagcttggc aagactcctg                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  human TRAC spacer sequence G2 

<400>  56
acaaaactgt gctagacatg                                                   20


<210>  57
<211>  18
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  human TRAC spacer sequence G4

<400>  57
tcaagagcaa cagtgctg                                                     18


<210>  58
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic polynucleotide

<220>
<221>  misc_feature
<223>  spacer sequence

<400>  58
ccgatgccca  accccaggcc                                                   20