                         SEQUENCE LISTING

<110>  Ecole Polytechnique Fdrale de Lausanne (EPFL)
 
<120>  A METHOD FOR PREPARING PHFS-LIKE TAU AGGREGATES

<130>  51872/WO

<150>  EP18169898.6
<151>  2018-04-27

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  PRT
<213>  Homo sapiens

<400>  1

Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
1               5                   10                  15      


Cys Gly Ser Lys Asp Asn Ile Lys His Val 
            20                  25      


<210>  2
<211>  57
<212>  PRT
<213>  Homo sapiens

<400>  2

Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val Lys Ser Lys 
1               5                   10                  15      


Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly Gly Lys Val 
            20                  25                  30          


Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys Cys 
        35                  40                  45              


Gly Ser Lys Asp Asn Ile Lys His Val 
    50                  55          


<210>  3
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  3

Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
1               5                   10                  15      


Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly 
            20                  25                  30  


<210>  4
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  4

Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
1               5                   10                  15      


Cys Gly Ser Lys Asp Asn Ile Lys His Val Ser Gly Gly Gly 
            20                  25                  30  


<210>  5
<211>  441
<212>  PRT
<213>  Homo sapiens

<400>  5

Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      


Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          


Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Glu Ser Pro Leu 
        35                  40                  45              


Gln Thr Pro Thr Glu Asp Gly Ser Glu Glu Pro Gly Ser Glu Thr Ser 
    50                  55                  60                  


Asp Ala Lys Ser Thr Pro Thr Ala Glu Asp Val Thr Ala Pro Leu Val 
65                  70                  75                  80  


Asp Glu Gly Ala Pro Gly Lys Gln Ala Ala Ala Gln Pro His Thr Glu 
                85                  90                  95      


Ile Pro Glu Gly Thr Thr Ala Glu Glu Ala Gly Ile Gly Asp Thr Pro 
            100                 105                 110         


Ser Leu Glu Asp Glu Ala Ala Gly His Val Thr Gln Ala Arg Met Val 
        115                 120                 125             


Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp Asp Lys Lys Ala Lys Gly 
    130                 135                 140                 


Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro Arg Gly Ala Ala Pro Pro 
145                 150                 155                 160 


Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg Ile Pro Ala Lys Thr Pro 
                165                 170                 175     


Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly Glu Pro Pro Lys Ser Gly 
            180                 185                 190         


Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser Pro Gly Thr Pro Gly Ser 
        195                 200                 205             


Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro Pro Thr Arg Glu Pro Lys 
    210                 215                 220                 


Lys Val Ala Val Val Arg Thr Pro Pro Lys Ser Pro Ser Ser Ala Lys 
225                 230                 235                 240 


Ser Arg Leu Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val 
                245                 250                 255     


Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly 
            260                 265                 270         


Gly Lys Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln 
        275                 280                 285             


Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly 
    290                 295                 300                 


Ser Val Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser 
305                 310                 315                 320 


Lys Cys Gly Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln 
                325                 330                 335     


Val Glu Val Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser 
            340                 345                 350         


Lys Ile Gly Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn 
        355                 360                 365             


Lys Lys Ile Glu Thr His Lys Leu Thr Phe Arg Glu Asn Ala Lys Ala 
    370                 375                 380                 


Lys Thr Asp His Gly Ala Glu Ile Val Tyr Lys Ser Pro Val Val Ser 
385                 390                 395                 400 


Gly Asp Thr Ser Pro Arg His Leu Ser Asn Val Ser Ser Thr Gly Ser 
                405                 410                 415     


Ile Asp Met Val Asp Ser Pro Gln Leu Ala Thr Leu Ala Asp Glu Val 
            420                 425                 430         


Ser Ala Ser Leu Ala Lys Gln Gly Leu 
        435                 440     


<210>  6
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  6

Gln Thr Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val Lys Ser Lys 
1               5                   10                  15      


Ile Gly Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly Gly Lys Val 
            20                  25                  30          


Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys Cys 
        35                  40                  45              


Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly Ser Val Gln 
    50                  55                  60                  


Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser Lys Cys Gly 
65                  70                  75                  80  


Ser Leu Gly Asn Ile His His Lys Pro Gly Gly Gly Gln Val Glu Val 
                85                  90                  95      


Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser Lys Ile Gly 
            100                 105                 110         


Ser Leu Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn Lys Lys Ile 
        115                 120                 125             


Glu 
    


<210>  7
<211>  412
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      


Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          


Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Glu Ser Pro Leu 
        35                  40                  45              


Gln Thr Pro Thr Glu Asp Gly Ser Glu Glu Pro Gly Ser Glu Thr Ser 
    50                  55                  60                  


Asp Ala Lys Ser Thr Pro Thr Ala Glu Ala Glu Glu Ala Gly Ile Gly 
65                  70                  75                  80  


Asp Thr Pro Ser Leu Glu Asp Glu Ala Ala Gly His Val Thr Gln Ala 
                85                  90                  95      


Arg Met Val Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp Asp Lys Lys 
            100                 105                 110         


Ala Lys Gly Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro Arg Gly Ala 
        115                 120                 125             


Ala Pro Pro Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg Ile Pro Ala 
    130                 135                 140                 


Lys Thr Pro Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly Glu Pro Pro 
145                 150                 155                 160 


Lys Ser Gly Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser Pro Gly Thr 
                165                 170                 175     


Pro Gly Ser Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro Pro Thr Arg 
            180                 185                 190         


Glu Pro Lys Lys Val Ala Val Val Arg Thr Pro Pro Lys Ser Pro Ser 
        195                 200                 205             


Ser Ala Lys Ser Arg Leu Gln Thr Ala Pro Val Pro Met Pro Asp Leu 
    210                 215                 220                 


Lys Asn Val Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu Lys His Gln 
225                 230                 235                 240 


Pro Gly Gly Gly Lys Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser 
                245                 250                 255     


Asn Val Gln Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro 
            260                 265                 270         


Gly Gly Gly Ser Val Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys 
        275                 280                 285             


Val Thr Ser Lys Cys Gly Ser Leu Gly Asn Ile His His Lys Pro Gly 
    290                 295                 300                 


Gly Gly Gln Val Glu Val Lys Ser Glu Lys Leu Asp Phe Lys Asp Arg 
305                 310                 315                 320 


Val Gln Ser Lys Ile Gly Ser Leu Asp Asn Ile Thr His Val Pro Gly 
                325                 330                 335     


Gly Gly Asn Lys Lys Ile Glu Thr His Lys Leu Thr Phe Arg Glu Asn 
            340                 345                 350         


Ala Lys Ala Lys Thr Asp His Gly Ala Glu Ile Val Tyr Lys Ser Pro 
        355                 360                 365             


Val Val Ser Gly Asp Thr Ser Pro Arg His Leu Ser Asn Val Ser Ser 
    370                 375                 380                 


Thr Gly Ser Ile Asp Met Val Asp Ser Pro Gln Leu Ala Thr Leu Ala 
385                 390                 395                 400 


Asp Glu Val Ser Ala Ser Leu Ala Lys Gln Gly Leu 
                405                 410         


<210>  8
<211>  383
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Ala Glu Pro Arg Gln Glu Phe Glu Val Met Glu Asp His Ala Gly 
1               5                   10                  15      


Thr Tyr Gly Leu Gly Asp Arg Lys Asp Gln Gly Gly Tyr Thr Met His 
            20                  25                  30          


Gln Asp Gln Glu Gly Asp Thr Asp Ala Gly Leu Lys Ala Glu Glu Ala 
        35                  40                  45              


Gly Ile Gly Asp Thr Pro Ser Leu Glu Asp Glu Ala Ala Gly His Val 
    50                  55                  60                  


Thr Gln Ala Arg Met Val Ser Lys Ser Lys Asp Gly Thr Gly Ser Asp 
65                  70                  75                  80  


Asp Lys Lys Ala Lys Gly Ala Asp Gly Lys Thr Lys Ile Ala Thr Pro 
                85                  90                  95      


Arg Gly Ala Ala Pro Pro Gly Gln Lys Gly Gln Ala Asn Ala Thr Arg 
            100                 105                 110         


Ile Pro Ala Lys Thr Pro Pro Ala Pro Lys Thr Pro Pro Ser Ser Gly 
        115                 120                 125             


Glu Pro Pro Lys Ser Gly Asp Arg Ser Gly Tyr Ser Ser Pro Gly Ser 
    130                 135                 140                 


Pro Gly Thr Pro Gly Ser Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro 
145                 150                 155                 160 


Pro Thr Arg Glu Pro Lys Lys Val Ala Val Val Arg Thr Pro Pro Lys 
                165                 170                 175     


Ser Pro Ser Ser Ala Lys Ser Arg Leu Gln Thr Ala Pro Val Pro Met 
            180                 185                 190         


Pro Asp Leu Lys Asn Val Lys Ser Lys Ile Gly Ser Thr Glu Asn Leu 
        195                 200                 205             


Lys His Gln Pro Gly Gly Gly Lys Val Gln Ile Ile Asn Lys Lys Leu 
    210                 215                 220                 


Asp Leu Ser Asn Val Gln Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys 
225                 230                 235                 240 


His Val Pro Gly Gly Gly Ser Val Gln Ile Val Tyr Lys Pro Val Asp 
                245                 250                 255     


Leu Ser Lys Val Thr Ser Lys Cys Gly Ser Leu Gly Asn Ile His His 
            260                 265                 270         


Lys Pro Gly Gly Gly Gln Val Glu Val Lys Ser Glu Lys Leu Asp Phe 
        275                 280                 285             


Lys Asp Arg Val Gln Ser Lys Ile Gly Ser Leu Asp Asn Ile Thr His 
    290                 295                 300                 


Val Pro Gly Gly Gly Asn Lys Lys Ile Glu Thr His Lys Leu Thr Phe 
305                 310                 315                 320 


Arg Glu Asn Ala Lys Ala Lys Thr Asp His Gly Ala Glu Ile Val Tyr 
                325                 330                 335     


Lys Ser Pro Val Val Ser Gly Asp Thr Ser Pro Arg His Leu Ser Asn 
            340                 345                 350         


Val Ser Ser Thr Gly Ser Ile Asp Met Val Asp Ser Pro Gln Leu Ala 
        355                 360                 365             


Thr Leu Ala Asp Glu Val Ser Ala Ser Leu Ala Lys Gln Gly Leu 
    370                 375                 380             


<210>  9
<211>  430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  9

Met Ala Asp Pro Arg Gln Glu Phe Asp Thr Met Glu Asp His Ala Gly 
1               5                   10                  15      


Asp Tyr Thr Leu Leu Gln Asp Gln Glu Gly Asp Met Asp His Gly Leu 
            20                  25                  30          


Lys Glu Ser Pro Pro Gln Pro Pro Ala Asp Asp Gly Ala Glu Glu Pro 
        35                  40                  45              


Gly Ser Glu Thr Ser Asp Ala Lys Ser Thr Pro Thr Ala Glu Asp Val 
    50                  55                  60                  


Thr Ala Pro Leu Val Asp Glu Arg Ala Pro Asp Lys Gln Ala Ala Ala 
65                  70                  75                  80  


Gln Pro His Thr Glu Ile Pro Glu Gly Ile Thr Ala Glu Glu Ala Gly 
                85                  90                  95      


Ile Gly Asp Thr Pro Asn Gln Glu Asp Gln Ala Ala Gly His Val Thr 
            100                 105                 110         


Gln Ala Arg Val Ala Ser Lys Asp Arg Thr Gly Asn Asp Glu Lys Lys 
        115                 120                 125             


Ala Lys Gly Ala Asp Gly Lys Thr Gly Ala Lys Ile Ala Thr Pro Arg 
    130                 135                 140                 


Gly Ala Ala Ser Pro Ala Gln Lys Gly Thr Ser Asn Ala Thr Arg Ile 
145                 150                 155                 160 


Pro Ala Lys Thr Thr Pro Ser Pro Lys Thr Pro Pro Gly Ser Gly Glu 
                165                 170                 175     


Pro Pro Lys Ser Gly Glu Arg Ser Gly Tyr Ser Ser Pro Gly Ser Pro 
            180                 185                 190         


Gly Thr Pro Gly Ser Arg Ser Arg Thr Pro Ser Leu Pro Thr Pro Pro 
        195                 200                 205             


Thr Arg Glu Pro Lys Lys Val Ala Val Val Arg Thr Pro Pro Lys Ser 
    210                 215                 220                 


Pro Ser Ala Ser Lys Ser Arg Leu Gln Thr Ala Pro Val Pro Met Pro 
225                 230                 235                 240 


Asp Leu Lys Asn Val Arg Ser Lys Ile Gly Ser Thr Glu Asn Leu Lys 
                245                 250                 255     


His Gln Pro Gly Gly Gly Lys Val Gln Ile Ile Asn Lys Lys Leu Asp 
            260                 265                 270         


Leu Ser Asn Val Gln Ser Lys Cys Gly Ser Lys Asp Asn Ile Lys His 
        275                 280                 285             


Val Pro Gly Gly Gly Ser Val Gln Ile Val Tyr Lys Pro Val Asp Leu 
    290                 295                 300                 


Ser Lys Val Thr Ser Lys Cys Gly Ser Leu Gly Asn Ile His His Lys 
305                 310                 315                 320 


Pro Gly Gly Gly Gln Val Glu Val Lys Ser Glu Lys Leu Asp Phe Lys 
                325                 330                 335     


Asp Arg Val Gln Ser Lys Ile Gly Ser Leu Asp Asn Ile Thr His Val 
            340                 345                 350         


Pro Gly Gly Gly Asn Lys Lys Ile Glu Thr His Lys Leu Thr Phe Arg 
        355                 360                 365             


Glu Asn Ala Lys Ala Lys Thr Asp His Gly Ala Glu Ile Val Tyr Lys 
    370                 375                 380                 


Ser Pro Val Val Ser Gly Asp Thr Ser Pro Arg His Leu Ser Asn Val 
385                 390                 395                 400 


Ser Ser Thr Gly Ser Ile Asp Met Val Asp Ser Pro Gln Leu Ala Thr 
                405                 410                 415     


Leu Ala Asp Glu Val Ser Ala Ser Leu Ala Lys Gln Gly Leu 
            420                 425                 430 


<210>  10
<211>  372
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  10

Met Ala Asp Pro Arg Gln Glu Phe Asp Thr Met Glu Asp His Ala Gly 
1               5                   10                  15      


Asp Tyr Thr Leu Leu Gln Asp Gln Glu Gly Asp Met Asp His Gly Leu 
            20                  25                  30          


Lys Ala Glu Glu Ala Gly Ile Gly Asp Thr Pro Asn Gln Glu Asp Gln 
        35                  40                  45              


Ala Ala Gly His Val Thr Gln Ala Arg Val Ala Ser Lys Asp Arg Thr 
    50                  55                  60                  


Gly Asn Asp Glu Lys Lys Ala Lys Gly Ala Asp Gly Lys Thr Gly Ala 
65                  70                  75                  80  


Lys Ile Ala Thr Pro Arg Gly Ala Ala Ser Pro Ala Gln Lys Gly Thr 
                85                  90                  95      


Ser Asn Ala Thr Arg Ile Pro Ala Lys Thr Thr Pro Ser Pro Lys Thr 
            100                 105                 110         


Pro Pro Gly Ser Gly Glu Pro Pro Lys Ser Gly Glu Arg Ser Gly Tyr 
        115                 120                 125             


Ser Ser Pro Gly Ser Pro Gly Thr Pro Gly Ser Arg Ser Arg Thr Pro 
    130                 135                 140                 


Ser Leu Pro Thr Pro Pro Thr Arg Glu Pro Lys Lys Val Ala Val Val 
145                 150                 155                 160 


Arg Thr Pro Pro Lys Ser Pro Ser Ala Ser Lys Ser Arg Leu Gln Thr 
                165                 170                 175     


Ala Pro Val Pro Met Pro Asp Leu Lys Asn Val Arg Ser Lys Ile Gly 
            180                 185                 190         


Ser Thr Glu Asn Leu Lys His Gln Pro Gly Gly Gly Lys Val Gln Ile 
        195                 200                 205             


Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys Cys Gly Ser 
    210                 215                 220                 


Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly Ser Val Gln Ile Val 
225                 230                 235                 240 


Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser Lys Cys Gly Ser Leu 
                245                 250                 255     


Gly Asn Ile His His Lys Pro Gly Gly Gly Gln Val Glu Val Lys Ser 
            260                 265                 270         


Glu Lys Leu Asp Phe Lys Asp Arg Val Gln Ser Lys Ile Gly Ser Leu 
        275                 280                 285             


Asp Asn Ile Thr His Val Pro Gly Gly Gly Asn Lys Lys Ile Glu Thr 
    290                 295                 300                 


His Lys Leu Thr Phe Arg Glu Asn Ala Lys Ala Lys Thr Asp His Gly 
305                 310                 315                 320 


Ala Glu Ile Val Tyr Lys Ser Pro Val Val Ser Gly Asp Thr Ser Pro 
                325                 330                 335     


Arg His Leu Ser Asn Val Ser Ser Thr Gly Ser Ile Asp Met Val Asp 
            340                 345                 350         


Ser Pro Gln Leu Ala Thr Leu Ala Asp Glu Val Ser Ala Ser Leu Ala 
        355                 360                 365             


Lys Gln Gly Leu 
    370         


<210>  11
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  11

Val Lys Ile Ile Asn Lys 
1               5       


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  12

Pro Pro Gly Gln Lys 
1               5   


<210>  13
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  13

Lys Leu Asp Leu Ser Asn Val Gln Ser Lys Cys Gly Ser Lys Asp Asn 
1               5                   10                  15      


Ile Lys His Val 
            20  


<210>  14
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  14

Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
1               5                   10                  15      


Cys 
    


<210>  15
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  15

Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser 
1               5                   10      


<210>  16
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  16

Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
1               5                   10                  15      


<210>  17
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  17

Val Gln Ile Ile Asn Asn Lys Leu Asp Leu Ser 
1               5                   10      


<210>  18
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  18

Val Gln Ile Ile Asn Lys Asn Leu Asp Leu Ser 
1               5                   10      


<210>  19
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  19

Val Gln Ile Ile Asn Lys Lys Leu Asp Leu Ser Asn Val Gln Ser Lys 
1               5                   10                  15      


Cys Gly Ser Lys Asp Asn Ile Lys His Val Pro Gly Gly Gly Ser Val 
            20                  25                  30          


Gln Ile Val Tyr Lys Pro Val Asp Leu Ser Lys Val Thr Ser Lys Cys 
        35                  40                  45              


Gly Ser Leu Gly Asn Ile His His Lys 
    50                  55          


<210>  20
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic peptide

<400>  20

Val Gln Ile Ile Asn Lys Leu Asp Leu Ser Asn Val Gln Ser Lys Cys 
1               5                   10                  15      


Gly Ser Lys Asp Asn Ile Lys His Val 
            20                  25  


