                         SEQUENCE LISTING

<110>  Shanghai Veterinary Research Institute, 
       Chinese Academy of Agricultural Sciences (National Center For Animal Health, Shanghai)
 
<120>  A COMPOSITE MULTI-EPITOPE EXPRESSION CASSETTE, 
       A RECOMBINANT VIRUS COMPOSED THEREOF 
       AND APPLICATION THEREOF

<160>  27    

<170>  PatentIn version 3.3

<210>  1
<211>  8
<212>  PRT
<213>  Avian infectious bronchitis virus

<400>  1

Gly Ala Tyr Ala Val Val Asn Val 
1               5               


<210>  2
<211>  9
<212>  PRT
<213>  Avian infectious bronchitis virus

<400>  2

Ser Arg Ile Gln Thr Ala Thr Gln Pro 
1               5                   


<210>  3
<211>  9
<212>  PRT
<213>  Avian infectious bronchitis virus

<400>  3

Ser Arg Ile Gln Thr Ala Thr Asp Pro 
1               5                   


<210>  4
<211>  9
<212>  PRT
<213>  Avian infectious bronchitis virus

<400>  4

Ser Arg Asn Ala Thr Gly Ser Gln Pro 
1               5                   


<210>  5
<211>  37
<212>  PRT
<213>  Avian infectious bronchitis virus

<400>  5

Asn Tyr Val Tyr Tyr Tyr Gln Ser Ala Phe Arg Pro Ser Gly Gly Trp 
1               5                   10                  15      


His Leu His Gly Gly Ala Tyr Ala Val Val Asn Val Ser Gln Glu Thr 
            20                  25                  30          


Ser Asn Ala Gly Ser 
        35          


<210>  6
<211>  17
<212>  PRT
<213>  Avian infectious bronchitis virus

<400>  6

Arg Ile Ala Ala Met Lys Gln Gly Gly Asn Gly Pro Ser Asp Leu Phe 
1               5                   10                  15      


Tyr 
    


<210>  7
<211>  107
<212>  PRT
<213>  Avian infectious bronchitis virus

<400>  7

Gln Thr Tyr Gln Thr Gln Thr Ala Gln Ser Gly Tyr Tyr Asn Phe Asn 
1               5                   10                  15      


Phe Ser Phe Leu Ser Gly Phe Val Tyr Lys Glu Phe Asn Phe Met Tyr 
            20                  25                  30          


Gly Ser Tyr His Pro Lys Cys Asn Phe Arg Pro Glu Asn Ile Asn Asn 
        35                  40                  45              


Gly Leu Trp Phe Asn Ser Leu Ser Val Ser Leu Ala Tyr Gly Pro Leu 
    50                  55                  60                  


Gln Gly Gly Cys Lys Gln Ser Val Phe His Gly Arg Ala Thr Cys Cys 
65                  70                  75                  80  


Tyr Ala Tyr Ser Tyr Leu Gly Pro Arg Leu Cys Lys Gly Val Tyr Ser 
                85                  90                  95      


Gly Glu Leu Thr Gln Gln Phe Glu Cys Gly Leu 
            100                 105         


<210>  8
<211>  9
<212>  DNA
<213>  Artificial sequence

<220>
<223>  linker sequence

<400>  8
aaagctgct                                                              9


<210>  9
<211>  9
<212>  DNA
<213>  Artificial sequence

<220>
<223>  linker sequence

<400>  9
gccgcatac                                                              9


<210>  10
<211>  9
<212>  DNA
<213>  Artificial sequence

<220>
<223>  linker 

<400>  10
gctgccgcc                                                              9


<210>  11
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  linker sequence

<400>  11
ggcgcagcag cc                                                         12


<210>  12
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  linker sequence

<400>  12
aaagcagccg ca                                                         12


<210>  13
<211>  9
<212>  DNA
<213>  Artificial sequence

<220>
<223>  linker sequence

<400>  13
gccaccatg                                                              9


<210>  14
<211>  331
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of the expression cassette 
       of the present application

<400>  14

Met Gly Asn Tyr Val Tyr Tyr Tyr Gln Ser Ala Phe Arg Pro Ser Gly 
1               5                   10                  15      


Gly Trp His Leu His Gly Gly Ala Tyr Ala Val Val Asn Val Ser Gln 
            20                  25                  30          


Glu Thr Ser Asn Ala Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        35                  40                  45              


Ser Gly Gly Gly Gly Ser Arg Ile Ala Ala Met Lys Gln Gly Gly Asn 
    50                  55                  60                  


Gly Pro Ser Asp Leu Phe Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 
65                  70                  75                  80  


Ser Gly Gly Gly Gly Ser Gln Thr Tyr Gln Thr Gln Thr Ala Gln Ser 
                85                  90                  95      


Gly Tyr Tyr Asn Phe Asn Phe Ser Phe Leu Ser Gly Phe Val Tyr Lys 
            100                 105                 110         


Glu Phe Asn Phe Met Tyr Gly Ser Tyr His Pro Lys Cys Asn Phe Arg 
        115                 120                 125             


Pro Glu Asn Ile Asn Asn Gly Leu Trp Phe Asn Ser Leu Ser Val Ser 
    130                 135                 140                 


Leu Ala Tyr Gly Pro Leu Gln Gly Gly Cys Lys Gln Ser Val Phe His 
145                 150                 155                 160 


Gly Arg Ala Thr Cys Cys Tyr Ala Tyr Ser Tyr Leu Gly Pro Arg Leu 
                165                 170                 175     


Cys Lys Gly Val Tyr Ser Gly Glu Leu Thr Gln Gln Phe Glu Cys Gly 
            180                 185                 190         


Leu Thr Ser Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
        195                 200                 205             


Asn Pro Gly Pro Phe Phe Phe Met Gln Val Gln Ile Gln Ser Leu Phe 
    210                 215                 220                 


Leu Leu Leu Leu Trp Val Pro Gly Ser Arg Gly Lys Ala Ala Gly Ala 
225                 230                 235                 240 


Tyr Ala Val Val Asn Val Ala Ala Ala Ser Arg Ile Gln Thr Ala Thr 
                245                 250                 255     


Gln Pro Ala Ala Tyr Ser Arg Asn Glu Thr Asp Ser Gln Pro Gly Ala 
            260                 265                 270         


Ala Ala Ser Arg Asn Ala Thr Gly Ser Gln Pro Lys Ala Ala Gly Ala 
        275                 280                 285             


Tyr Ala Val Val Asn Val Ala Ala Ala Ser Arg Ile Gln Thr Ala Thr 
    290                 295                 300                 


Gln Pro Ala Ala Tyr Ser Arg Asn Glu Thr Asp Ser Gln Pro Gly Ala 
305                 310                 315                 320 


Ala Ala Ser Arg Asn Ala Thr Gly Ser Gln Pro 
                325                 330     


<210>  15
<211>  1032
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Nucleotide sequence of the expression cassette 
       of the present application

<400>  15
acgggtagaa agcttgccac catgggaaat tacgtttact actaccaaag tgccttcaga     60

ccatcaggtg gttggcattt acatggaggt gcttatgcag tagtaaatgt ttcgcaagaa    120

accagtaatg caggaagcgg aggcggaggc tccggaggag gaggctccgg aggcggaggg    180

tctcgtattg ctgccatgaa gcaaggcggt aatgggccta gtgatttatt ttatggaggc    240

ggaggctccg gaggaggagg ctccggaggc ggagggtctc aaacttatca aacacaaaca    300

gctcagagtg gttattataa ttttaacttc tcatttctga gtggttttgt gtataaggag    360

tttaatttta tgtatggttc ttatcaccca aagtgtaatt ttagaccaga aaacattaat    420

aatggcctct ggtttaattc actttcagtt tcgcttgcgt atggccctct tcaaggcggc    480

tgcaagcaat ctgtctttca tggtagagca acttgctgtt atgcctactc ctatttaggt    540

cctaggttat gtaaaggtgt ttatagtggt gagttaacac agcagtttga atgtggactg    600

actagtaact ttgacctgct caagttggca ggagacgtcg agtccaaccc tgggcctttc    660

ttcttcatgc aggtgcagat ccagagcctg tttctgctcc tcctgtgggt gcccggctcc    720

agaggaaaag ctgctggtgc atatgcagtc gtcaacgttg ctgccgccag taggattcag    780

acggctactc agccggccgc atacagtaga aatgagaccg atagtcagcc gggcgcagca    840

gccagtagaa acgctactgg tagtcaaccg aaagctgctg gtgcatatgc agtcgtcaac    900

gttgctgccg ccagtaggat tcagacggct actcagccgg ccgcatacag tagaaatgag    960

accgatagtc agccgggcgc agcagccagt agaaacgcta ctggtagtca accgtaataa   1020

ttaagaaaaa at                                                       1032


<210>  16
<211>  616
<212>  PRT
<213>  Newcastle disease virus TS09-C strain

<400>  16

Met Asp Arg Ala Val Ser Gln Val Ala Leu Glu Asn Asp Glu Arg Glu 
1               5                   10                  15      


Ala Lys Asn Thr Trp Arg Leu Val Phe Arg Ile Ala Ile Leu Leu Ser 
            20                  25                  30          


Thr Val Val Thr Leu Ala Ile Ser Ala Ala Ala Leu Ala Tyr Ser Met 
        35                  40                  45              


Glu Ala Ser Thr Pro Ser Asp Leu Val Gly Ile Pro Thr Ala Ile Ser 
    50                  55                  60                  


Arg Ala Glu Glu Lys Ile Thr Ser Ala Leu Gly Ser Asn Gln Asp Val 
65                  70                  75                  80  


Val Asp Arg Ile Tyr Lys Gln Val Ala Leu Glu Ser Pro Leu Ala Leu 
                85                  90                  95      


Leu Asn Thr Glu Ser Thr Ile Met Asn Ala Ile Thr Ser Leu Ser Tyr 
            100                 105                 110         


Gln Ile Ser Gly Ala Ala Ser Ser Ser Gly Cys Gly Ala Pro Ile His 
        115                 120                 125             


Asp Pro Asp Tyr Ile Gly Gly Ile Gly Lys Glu Leu Ile Val Asp Asp 
    130                 135                 140                 


Ala Ser Asp Val Thr Ser Tyr Tyr Pro Ser Ala Phe Gln Glu His Leu 
145                 150                 155                 160 


Asn Phe Ile Pro Ala Pro Thr Thr Gly Ser Gly Cys Thr Arg Met Pro 
                165                 170                 175     


Ser Phe Asp Met Ser Ala Thr His Tyr Cys Tyr Thr His Asn Val Ile 
            180                 185                 190         


Leu Ser Gly Cys Arg Asp His Ser His Ser His Gln Tyr Leu Ala Leu 
        195                 200                 205             


Gly Val Leu Arg Thr Ser Ala Thr Gly Arg Val Phe Phe Ser Thr Leu 
    210                 215                 220                 


Arg Ser Ile Asn Leu Asp Asp Thr Gln Asn Arg Lys Ser Cys Ser Val 
225                 230                 235                 240 


Ser Ala Thr Pro Leu Gly Cys Asp Met Leu Cys Ser Lys Val Thr Glu 
                245                 250                 255     


Thr Glu Glu Glu Asp Tyr Asn Ser Ala Ile Pro Thr Ser Met Val His 
            260                 265                 270         


Gly Arg Leu Gly Phe Asp Gly Gln Tyr His Glu Lys Asp Leu Asp Val 
        275                 280                 285             


Thr Thr Leu Phe Glu Asp Trp Val Ala Asn Tyr Pro Gly Val Gly Gly 
    290                 295                 300                 


Gly Ser Phe Ile Asp Asn Arg Val Trp Phe Pro Val Tyr Gly Gly Leu 
305                 310                 315                 320 


Lys Pro Asn Ser Pro Ser Asp Thr Ala Gln Glu Gly Lys Tyr Val Ile 
                325                 330                 335     


Tyr Lys Arg Tyr Asn Asp Thr Cys Pro Asp Glu Gln Asp Tyr Gln Ile 
            340                 345                 350         


Gln Met Ala Lys Ser Ser Tyr Lys Pro Gly Arg Phe Gly Gly Lys Arg 
        355                 360                 365             


Val Gln Gln Ala Val Leu Ser Ile Lys Val Ser Thr Ser Leu Gly Glu 
    370                 375                 380                 


Asp Pro Val Leu Thr Val Pro Pro Asn Thr Val Thr Leu Met Gly Ala 
385                 390                 395                 400 


Glu Gly Arg Val Leu Thr Val Gly Thr Ser His Phe Leu Tyr Gln Arg 
                405                 410                 415     


Gly Ser Ser Tyr Phe Ser Pro Ala Leu Leu Tyr Pro Met Ile Val Ser 
            420                 425                 430         


Asn Lys Thr Ala Thr Leu His Ser Pro Tyr Thr Phe Asn Ala Phe Thr 
        435                 440                 445             


Arg Pro Gly Ser Val Pro Cys Gln Ala Ser Ala Arg Cys Pro Asn Ser 
    450                 455                 460                 


Cys Val Thr Gly Val Tyr Thr Asp Pro Tyr Pro Leu Val Phe Tyr Arg 
465                 470                 475                 480 


Asn His Thr Leu Arg Gly Val Phe Gly Thr Met Leu Asp Asp Lys Gln 
                485                 490                 495     


Ala Arg Leu Asn Pro Val Ser Ala Val Phe Asp Ser Ile Ser Arg Ser 
            500                 505                 510         


Arg Ile Thr Arg Val Ser Ser Ser Ser Thr Lys Ala Ala Tyr Thr Thr 
        515                 520                 525             


Ser Thr Cys Phe Lys Val Val Lys Thr Asn Lys Thr Tyr Cys Leu Ser 
    530                 535                 540                 


Ile Ala Glu Ile Ser Asn Thr Leu Phe Gly Glu Phe Arg Ile Val Pro 
545                 550                 555                 560 


Leu Leu Val Glu Ile Leu Lys Asp Asp Gly Val Arg Glu Ala Arg Ser 
                565                 570                 575     


Ser Arg Leu Ser Gln Leu Arg Glu Gly Trp Lys Asp Asp Ile Val Ser 
            580                 585                 590         


Pro Ile Phe Cys Asp Ala Lys Asn Gln Thr Glu Tyr Arg His Glu Leu 
        595                 600                 605             


Glu Ser Tyr Ala Ala Ser Trp Pro 
    610                 615     


<210>  17
<211>  2002
<212>  DNA
<213>  Newcastle disease virus TS09-C strain

<400>  17
acgggtagaa cggtcgggga ggccgtccct caatcgggag ccgggcctca caacatccgt     60

tctaccgcat caccaatagc agttttcagt catggaccgc gcagttagcc aagttgcgct    120

agagaatgat gaaagagagg caaagaatac atggcgcttg gtattccgga tcgcaatcct    180

actctcaacg gtggtgacct tagccatctc tgcagccgcc cttgcatata gcatggaggc    240

cagcacacct agcgatcttg taggcatacc gactgcgatc tctagagcag aggaaaagat    300

tacatctgca ctcggttcca atcaagatgt agtagatagg atatataagc aggtggccct    360

cgaatctcca ctggcattgc taaacaccga atctacaatt atgaacgcaa taacgtctct    420

ctcttatcaa atcagtgggg ccgcaagtag cagcggatgt ggagcaccca ttcatgatcc    480

agattatatt ggaggaatag gtaaagaact tattgtagat gatgctagcg acgtcacatc    540

atactatccc tctgcgttcc aagaacacct gaactttatc ccggcgccta ctacaggatc    600

aggttgcact cggatgccct catttgacat gagcgctacc cactactgtt atactcacaa    660

tgtgatatta tctggctgca gagatcactc gcactcacat caatatttag cacttggtgt    720

gcttcggaca tctgcaacag ggagggtatt cttttccact ctgcgttcca tcaatctgga    780

tgacacccaa aatcggaagt cttgcagtgt gagtgcaacc cccttgggtt gtgatatgct    840

gtgctctaaa gtcacagaga ctgaagaaga ggattataac tcagctatcc ccacgtcgat    900

ggtacatgga aggttagggt tcgacggcca ataccacgag aaggacctag atgtcacaac    960

actattcgag gactgggtgg caaactaccc aggagtagga ggcgggtctt ttattgacaa   1020

ccgcgtatgg ttcccagttt acggagggct aaaacccaat tcgcccagtg acaccgcaca   1080

agaagggaaa tatgtaatat acaagcgata caatgacaca tgtccagatg agcaagatta   1140

tcagattcaa atggctaagt cttcatataa gcctgggcgg tttggaggga aacgcgtaca   1200

gcaggccgtc ttatctatca aagtgtcaac atccttgggc gaggacccgg tgctgactgt   1260

accgcccaac acagtaacac tcatgggggc cgaaggcaga gttctcacag tagggacatc   1320

tcatttcctt tatcagcgag ggtcatcata cttctcccct gccctactat atcctatgat   1380

agtcagcaac aaaacagcca ctcttcatag tccttataca ttcaatgcct tcactcgacc   1440

aggtagtgtc ccttgccagg cttcagcaag atgccctaac tcatgtgtta ccggagtcta   1500

tactgatcca tatcccttgg tcttctatag gaaccacacc ttgcgagggg tattcgggac   1560

gatgcttgat gataaacaag caagactcaa ccctgtatct gcagtatttg acagcatatc   1620

ccgcagtcgc ataacccggg tgagttcaag cagcaccaag gcagcataca caacatcaac   1680

atgttttaaa gttgtaaaga ccaataaaac ctattgtctc agcattgccg aaatatccaa   1740

taccctcttc ggggaattca gaatcgtccc tttactagtt gagattctca aggatgatgg   1800

ggttagagaa gccaggtcta gccggttgag tcaactgcga gagggttgga aagatgacat   1860

tgtatcacct atcttttgcg acgccaagaa tcaaactgaa taccggcacg agctcgagtc   1920

ctacgctgcc agttggccat aatcagctag tgctaatgtg attagattaa gtcttgtcgg   1980

tagtcacttg attaagaaaa aa                                            2002


<210>  18
<211>  48
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  18
taatacgact cactataggg agaaccaaac agagaatctg tgagttac                  48


<210>  19
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  19
aactcagtgc caacatgact cggac                                           25


<210>  20
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  20
tcccggtcgg cgccttcaag gtgca                                           25


<210>  21
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  21
tctgatgctc cgccctctcg ggacc                                           25


<210>  22
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  22
aaaaatgtgg gtggtagcgg gatat                                           25


<210>  23
<211>  113
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  23
accaaacaaa gatttggtga atgacaataa ctagcataac cccttggggc ctctaaacgg     60

gtcttgatgg ccggcatggt cccagcctcc tcgctggcgc cggctgggca aca           113


<210>  24
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  24
atggaccgcg cagttagcca agttg                                           25


<210>  25
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  25
ttatggccaa ctggcagcgt aggac                                           25


<210>  26
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  26
cactcggcat cacacggaat c                                               21


<210>  27
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer

<400>  27
gtccacaagt caaggcgctg                                                 20


