                         SEQUENCE LISTING

<110>  Pacific Northwest Research Institute
       Sirr , Amy
       Scott, Adrian  C.
       Dudley , Aimee  M.
 
<120>  METHODS OF DETECTING AMINO ACID DEFICIENCIES

<130>  PNDRI.008WO

<150>  62/659307
<151>  2018-04-18

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  1146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon optimized PSAT1

<400>  1
atggacgcgc caagacaggt tgtgaatttc ggacctgggc cagctaaatt gcctcactct       60

gttttgttgg aaatccagaa agagttatta gattataaag gtgtaggtat atcagtcttg      120

gaaatgtctc accgttcgtc tgatttcgcc aaaataatta ataacaccga gaacttagtt      180

agggaactgc tagccgttcc tgacaactat aaagtgattt ttttgcaagg tggtggctgc      240

ggtcaatttt ctgccgttcc tttaaatttg ataggtttaa aagcgggtag atgtgccgac      300

tatgtcgtca ccggtgcgtg gtctgctaaa gccgctgaag aggcaaagaa attcggtact      360

atcaacattg ttcatccaaa gttgggctct tacactaaaa ttccggatcc atccacatgg      420

aatttaaatc ccgatgcatc atacgtgtac tactgtgcta atgaaactgt tcatggtgtt      480

gaatttgact ttattccaga tgttaagggt gctgttttgg tttgcgacat gtccagcaac      540

ttcttgtcta agccagttga cgtttctaaa ttcggtgtca tctttgcagg cgcccaaaaa      600

aacgttggtt ctgcaggcgt aaccgtggtt attgtcagag atgacttact tggttttgcg      660

ttaagagaat gcccttctgt tttagagtat aaggtgcagg ccggtaactc atcactgtat      720

aatacaccac catgcttctc tatatatgtt atgggtctcg tgttagaatg gattaagaat      780

aatggtggtg cagctgcgat ggagaaattg tcttctatta agtctcagac gatatatgag      840

ataatagata actcacaggg tttttacgtt tgcccagtgg aaccacagaa tagatcaaaa      900

atgaatatcc cattccgtat aggtaacgcg aagggcgatg acgcccttga aaagaggttt      960

ctagataagg cacttgagtt aaatatgctg tctttgaaag gacatcgttc agttggtggg     1020

atcagagcct ccttgtataa cgcggtgacg atcgaagacg tacaaaagtt ggccgctttt     1080

atgaagaaat ttttggagat gcaccagtta taaatgaaga aatttttgga gatgcaccag     1140

ttataa                                                                1146


<210>  2
<211>  1146
<212>  DNA
<213>  Homo sapiens

<400>  2
atggacgccc ccaggcaggt ggtcaacttt gggcctggtc ccgccaagct gccgcactca       60

gtgttgttag agatacaaaa ggaattatta gactacaaag gagttggcat tagtgttctt      120

gaaatgagtc acaggtcatc agattttgcc aagattatta acaatacaga gaatcttgtg      180

cgggaattgc tagctgttcc agacaactat aaggtgattt ttctgcaagg aggtgggtgc      240

ggccagttca gtgctgtccc cttaaacctc attggcttga aagcaggaag gtgtgcggac      300

tatgtggtga caggagcttg gtcagctaag gccgcagaag aagccaagaa gtttgggact      360

ataaatatcg ttcaccctaa acttgggagt tatacaaaaa ttccagatcc aagcacctgg      420

aacctcaacc cagatgcctc ctacgtgtat tattgcgcaa atgagacggt gcatggtgtg      480

gagtttgact ttatacccga tgtcaaggga gcagtactgg tttgtgacat gtcctcaaac      540

ttcctgtcca agccagtgga tgtttccaag tttggtgtga tttttgctgg tgcccagaag      600

aatgttggct ctgctggggt caccgtggtg attgtccgtg atgacctgct ggggtttgcc      660

ctccgagagt gcccctcggt cctggaatac aaggtgcagg ctggaaacag ctccttgtac      720

aacacgcctc catgtttcag catctacgtc atgggcttgg ttctggagtg gattaaaaac      780

aatggaggtg ccgcggccat ggagaagctt agctccatca aatctcaaac aatttatgag      840

attattgata attctcaagg attctacgtt tgtccagtgg agccccaaaa tagaagcaag      900

atgaatattc cattccgcat tggcaatgcc aaaggagatg atgctttaga aaaaagattt      960

cttgataaag ctcttgaact caatatgttg tccttgaaag ggcataggtc tgtgggaggc     1020

atccgggcct ctctgtataa tgctgtcaca attgaagacg ttcagaagct ggccgccttc     1080

atgaaaaaat ttttggagat gcatcagcta tgaatgaaaa aatttttgga gatgcatcag     1140

ctatga                                                                1146


<210>  3
<211>  7210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid

<400>  3
acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc       60

gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag      120

ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca      180

tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa      240

ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga      300

tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata      360

attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca      420

agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg      480

ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg      540

ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg      600

cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag      660

gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac      720

tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca      780

tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag      840

tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta      900

tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc      960

agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc     1020

agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc     1080

agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa     1140

aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg     1200

tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa     1260

gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg     1320

agctcggtac ccggggctct tcgacaaaga cattaaggag cctttgaaat tacttaattg     1380

aacaacacag gtctaattag ttgatcaatc atcgattaac cattagtgat aagaaacaat     1440

ggacgcgcca agacaggttg tgaatttcgg acctgggcca gctaaattgc ctcactctgt     1500

tttgttggaa atccagaaag agttattaga ttataaaggt gtaggtatat cagtcttgga     1560

aatgtctcac cgttcgtctg atttcgccaa aataattaat aacaccgaga acttagttag     1620

ggaactgcta gccgttcctg acaactataa agtgattttt ttgcaaggtg gtggctgcgg     1680

tcaattttct gccgttcctt taaatttgat aggtttaaaa gcgggtagat gtgccgacta     1740

tgtcgtcacc ggtgcgtggt ctgctaaagc cgctgaagag gcaaagaaat tcggtactat     1800

caacattgtt catccaaagt tgggctctta cactaaaatt ccggatccat ccacatggaa     1860

tttaaatccc gatgcatcat acgtgtacta ctgtgctaat gaaactgttc atggtgttga     1920

atttgacttt attccagatg ttaagggtgc tgttttggtt tgcgacatgt ccagcaactt     1980

cttgtctaag ccagttgacg tttctaaatt cggtgtcatc tttgcaggcg cccaaaaaaa     2040

cgttggttct gcaggcgtaa ccgtggttat tgtcagagat gacttacttg gttttgcgtt     2100

aagagaatgc ccttctgttt tagagtataa ggtgcaggcc ggtaactcat cactgtataa     2160

tacaccacca tgcttctcta tatatgttat gggtctcgtg ttagaatgga ttaagaataa     2220

tggtggtgca gctgcgatgg agaaattgtc ttctattaag tctcagacga tatatgagat     2280

aatagataac tcacagggtt tttacgtttg cccagtggaa ccacagaata gatcaaaaat     2340

gaatatccca ttccgtatag gtaacgcgaa gggcgatgac gcccttgaaa agaggtttct     2400

agataaggca cttgagttaa atatgctgtc tttgaaagga catcgttcag ttggtgggat     2460

cagagcctcc ttgtataacg cggtgacgat cgaagacgta caaaagttgg ccgcttttat     2520

gaagaaattt ttggagatgc accagttata atatcagcgg aatatgcctg taatttggtt     2580

caaagaaaaa ctttttcaag tcatatgtaa gttttaaata aatcagttta cgaacatgca     2640

caagaataac ttctcttctt tttcttgaaa taaaggccga attatacgat atgatatgag     2700

acgttctaat tacaatacga taccgtgaga taaaaatagc gatatcctcg tactcaaaga     2760

agtctaattg ttttagcccg ttgatcaaaa aaataaaatg tatttttatt tttttgtcat     2820

ccagctcaag atcgtaatat tggcaattaa caaccgagcg aggcacaggg caaagagaat     2880

gtgggtgaga ctgaactatc tctcccaata tattatactt ttttttgtcg cgtctaccaa     2940

ttaaatgaaa acataattgg gaatactttc ctcttcttat attttatctt aactcgtttc     3000

cgatcttctg atatcgtacg ctgcaggtcg acggatcccc gggttaatta aggcgcgcca     3060

gatctgttta gcttgccttg tccccgccgg gtcacccggc cagcgacatg gaggcccaga     3120

ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt     3180

acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca     3240

cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc     3300

tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg     3360

atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt     3420

ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc     3480

gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt     3540

cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt     3600

tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa     3660

ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga     3720

tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata     3780

tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc     3840

gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca     3900

atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg     3960

gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt     4020

cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg     4080

ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg     4140

gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat     4200

tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc     4260

agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat     4320

attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac     4380

atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg     4440

tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa     4500

acgagctcga attcatcgat aagaagtatc tcttttcaag aggattactt ttcattgaaa     4560

acttttggac gggcaaaaaa aaaaaaagcg ccagcaggaa gaaaaaagga atctagtaaa     4620

aaataaaaat taggtttata aagtagtaag tgaagtgcaa gagggagcgt tattggacga     4680

tcatgttgtg atcggatccc gtttattggt ctttgattca atttaaaaga aaagaaatgt     4740

acaggttaaa cttcaacata cgtatgcagg catgcaagct tggcgtaatc atggtcatag     4800

ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc     4860

ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc     4920

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa     4980

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg     5040

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     5100

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     5160

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     5220

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     5280

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     5340

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc     5400

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     5460

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     5520

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     5580

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca     5640

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     5700

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     5760

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     5820

cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     5880

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     5940

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     6000

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     6060

ttaccatctg gccccagtgc tgcaatgata ccgcgagatg gacgcgccaa gacaggttgt     6120

gaatttcgga cctgggccag ctaaattgcc tcactctgtt ttgttggaaa tccagaaaga     6180

gttattagat tataaaggtg taggtatatc agtcttggaa atgtctcacc gttcgtctga     6240

tttcgccaaa ataattaata acaccgagaa cttagttagg gaactgctag ccgttcctga     6300

caactataaa gtgatttttt tgcaaggtgg tggctgcggt caattttctg ccgttccttt     6360

aaatttgata ggtttaaaag cgggtagatg tgccgactat gtcgtcaccg gtgcgtggtc     6420

tgctaaagcc gctgaagagg caaagaaatt cggtactatc aacattgttc atccaaagtt     6480

gggctcttac actaaaattc cggatccatc cacatggaat ttaaatcccg atgcatcata     6540

cgtgtactac tgtgctaatg aaactgttca tggtgttgaa tttgacttta ttccagatgt     6600

taagggtgct gttttggttt gcgacatgtc cagcaacttc ttgtctaagc cagttgacgt     6660

ttctaaattc ggtgtcatct ttgcaggcgc ccaaaaaaac gttggttctg caggcgtaac     6720

cgtggttatt gtcagagatg acttacttgg ttttgcgtta agagaatgcc cttctgtttt     6780

agagtataag gtgcaggccg gtaactcatc actgtataat acaccaccat gcttctctat     6840

atatgttatg ggtctcgtgt tagaatggat taagaataat ggtggtgcag ctgcgatgga     6900

gaaattgtct tctattaagt ctcagacgat atatgagata atagataact cacagggttt     6960

ttacgtttgc ccagtggaac cacagaatag atcaaaaatg aatatcccat tccgtatagg     7020

taacgcgaag ggcgatgacg cccttgaaaa gaggtttcta gataaggcac ttgagttaaa     7080

tatgctgtct ttgaaaggac atcgttcagt tggtgggatc agagcctcct tgtataacgc     7140

ggtgacgatc gaagacgtac aaaagttggc cgcttttatg aagaaatttt tggagatgca     7200

ccagttataa                                                            7210


