               SEQUENCE LISTING

<110> Bayer Pharma Aktiengesellschaft
      Bayer Aktiengesellschaft

<120> 2-METHYL-AZA-QUINAZOLINES

<130> BHC 16 3 102

<160> 7

<170> BiSSAP 1.3.6

<210> 1
<211> 414
<212> PRT
<213> synthetic organisms


<220> 
<223> GST-hK-RasG12C (G12C mutation according to numbering in P01116-2)

<400> 1
Met Ser Pro Ile Leu Gly Tyr Trp Lys Ile Lys Gly Leu Val Gln Pro 
1               5                   10                  15      
Thr Arg Leu Leu Leu Glu Tyr Leu Glu Glu Lys Tyr Glu Glu His Leu 
            20                  25                  30          
Tyr Glu Arg Asp Glu Gly Asp Lys Trp Arg Asn Lys Lys Phe Glu Leu 
        35                  40                  45              
Gly Leu Glu Phe Pro Asn Leu Pro Tyr Tyr Ile Asp Gly Asp Val Lys 
    50                  55                  60                  
Leu Thr Gln Ser Met Ala Ile Ile Arg Tyr Ile Ala Asp Lys His Asn 
65                  70                  75                  80  
Met Leu Gly Gly Cys Pro Lys Glu Arg Ala Glu Ile Ser Met Leu Glu 
                85                  90                  95      
Gly Ala Val Leu Asp Ile Arg Tyr Gly Val Ser Arg Ile Ala Tyr Ser 
            100                 105                 110         
Lys Asp Phe Glu Thr Leu Lys Val Asp Phe Leu Ser Lys Leu Pro Glu 
        115                 120                 125             
Met Leu Lys Met Phe Glu Asp Arg Leu Cys His Lys Thr Tyr Leu Asn 
    130                 135                 140                 
Gly Asp His Val Thr His Pro Asp Phe Met Leu Tyr Asp Ala Leu Asp 
145                 150                 155                 160 
Val Val Leu Tyr Met Asp Pro Met Cys Leu Asp Ala Phe Pro Lys Leu 
                165                 170                 175     
Val Cys Phe Lys Lys Arg Ile Glu Ala Ile Pro Gln Ile Asp Lys Tyr 
            180                 185                 190         
Leu Lys Ser Ser Lys Tyr Ile Ala Trp Pro Leu Gln Gly Trp Gln Ala 
        195                 200                 205             
Thr Phe Gly Gly Gly Asp His Pro Pro Lys Ser Asp Pro Ile Thr Ser 
    210                 215                 220                 
Leu Tyr Lys Lys Ala Gly Ser Asp Tyr Asp Ile Pro Thr Thr Glu Asn 
225                 230                 235                 240 
Leu Tyr Phe Gln Gly Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala 
                245                 250                 255     
Cys Gly Val Gly Lys Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His 
            260                 265                 270         
Phe Val Asp Glu Tyr Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln 
        275                 280                 285             
Val Val Ile Asp Gly Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala 
    290                 295                 300                 
Gly Gln Glu Glu Tyr Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly 
305                 310                 315                 320 
Glu Gly Phe Leu Cys Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu 
                325                 330                 335     
Asp Ile His His Tyr Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Glu 
            340                 345                 350         
Asp Val Pro Met Val Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg 
        355                 360                 365             
Thr Val Asp Thr Lys Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile 
    370                 375                 380                 
Pro Phe Ile Glu Thr Ser Ala Lys Thr Arg Gln Gly Val Asp Asp Ala 
385                 390                 395                 400 
Phe Tyr Thr Leu Val Arg Glu Ile Arg Lys His Lys Glu Lys 
                405                 410                 

<210> 2
<211> 533
<212> PRT
<213> synthetic organisms


<220> 
<223> His10-hSOS1

<400> 2
Met Gly His His His His His His His His His His Ser Ser Gly His 
1               5                   10                  15      
Ile Glu Gly Arg His Met Leu Glu Thr Ser Leu Tyr Lys Lys Ala Gly 
            20                  25                  30          
Ser Asp Tyr Asp Ile Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Glu 
        35                  40                  45              
Glu Gln Met Arg Leu Pro Ser Ala Asp Val Tyr Arg Phe Ala Glu Pro 
    50                  55                  60                  
Asp Ser Glu Glu Asn Ile Ile Phe Glu Glu Asn Met Gln Pro Lys Ala 
65                  70                  75                  80  
Gly Ile Pro Ile Ile Lys Ala Gly Thr Val Ile Lys Leu Ile Glu Arg 
                85                  90                  95      
Leu Thr Tyr His Met Tyr Ala Asp Pro Asn Phe Val Arg Thr Phe Leu 
            100                 105                 110         
Thr Thr Tyr Arg Ser Phe Cys Lys Pro Gln Glu Leu Leu Ser Leu Ile 
        115                 120                 125             
Ile Glu Arg Phe Glu Ile Pro Glu Pro Glu Pro Thr Glu Ala Asp Arg 
    130                 135                 140                 
Ile Ala Ile Glu Asn Gly Asp Gln Pro Leu Ser Ala Glu Leu Lys Arg 
145                 150                 155                 160 
Phe Arg Lys Glu Tyr Ile Gln Pro Val Gln Leu Arg Val Leu Asn Val 
                165                 170                 175     
Cys Arg His Trp Val Glu His His Phe Tyr Asp Phe Glu Arg Asp Ala 
            180                 185                 190         
Tyr Leu Leu Gln Arg Met Glu Glu Phe Ile Gly Thr Val Arg Gly Lys 
        195                 200                 205             
Ala Met Lys Lys Trp Val Glu Ser Ile Thr Lys Ile Ile Gln Arg Lys 
    210                 215                 220                 
Lys Ile Ala Arg Asp Asn Gly Pro Gly His Asn Ile Thr Phe Gln Ser 
225                 230                 235                 240 
Ser Pro Pro Thr Val Glu Trp His Ile Ser Arg Pro Gly His Ile Glu 
                245                 250                 255     
Thr Phe Asp Leu Leu Thr Leu His Pro Ile Glu Ile Ala Arg Gln Leu 
            260                 265                 270         
Thr Leu Leu Glu Ser Asp Leu Tyr Arg Ala Val Gln Pro Ser Glu Leu 
        275                 280                 285             
Val Gly Ser Val Trp Thr Lys Glu Asp Lys Glu Ile Asn Ser Pro Asn 
    290                 295                 300                 
Leu Leu Lys Met Ile Arg His Thr Thr Asn Leu Thr Leu Trp Phe Glu 
305                 310                 315                 320 
Lys Cys Ile Val Glu Thr Glu Asn Leu Glu Glu Arg Val Ala Val Val 
                325                 330                 335     
Ser Arg Ile Ile Glu Ile Leu Gln Val Phe Gln Glu Leu Asn Asn Phe 
            340                 345                 350         
Asn Gly Val Leu Glu Val Val Ser Ala Met Asn Ser Ser Pro Val Tyr 
        355                 360                 365             
Arg Leu Asp His Thr Phe Glu Gln Ile Pro Ser Arg Gln Lys Lys Ile 
    370                 375                 380                 
Leu Glu Glu Ala His Glu Leu Ser Glu Asp His Tyr Lys Lys Tyr Leu 
385                 390                 395                 400 
Ala Lys Leu Arg Ser Ile Asn Pro Pro Cys Val Pro Phe Phe Gly Ile 
                405                 410                 415     
Tyr Leu Thr Asn Ile Leu Lys Thr Glu Glu Gly Asn Pro Glu Val Leu 
            420                 425                 430         
Lys Arg His Gly Lys Glu Leu Ile Asn Phe Ser Lys Arg Arg Lys Val 
        435                 440                 445             
Ala Glu Ile Thr Gly Glu Ile Gln Gln Tyr Gln Asn Gln Pro Tyr Cys 
    450                 455                 460                 
Leu Arg Val Glu Ser Asp Ile Lys Arg Phe Phe Glu Asn Leu Asn Pro 
465                 470                 475                 480 
Met Gly Asn Ser Met Glu Lys Glu Phe Thr Asp Tyr Leu Phe Asn Lys 
                485                 490                 495     
Ser Leu Glu Ile Glu Pro Arg Asn Pro Lys Pro Leu Pro Arg Phe Pro 
            500                 505                 510         
Lys Lys Tyr Ser Tyr Pro Leu Lys Ser Pro Gly Val Arg Pro Ser Asn 
        515                 520                 525             
Pro Arg Pro Gly Thr 
    530             

<210> 3
<211> 536
<212> PRT
<213> synthetic organisms


<220> 
<223> His10-hSOS1_12

<400> 3
Met Gly His His His His His His His His His His Ser Ser Gly His 
1               5                   10                  15      
Ile Glu Gly Arg His Met Leu Glu Thr Ser Leu Tyr Lys Lys Ala Gly 
            20                  25                  30          
Ser Asp Tyr Asp Ile Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Ala 
        35                  40                  45              
Met Ala Glu Glu Gln Met Arg Leu Pro Ser Ala Asp Val Tyr Arg Phe 
    50                  55                  60                  
Ala Glu Pro Asp Ser Glu Glu Asn Ile Ile Phe Glu Glu Asn Met Gln 
65                  70                  75                  80  
Pro Lys Ala Gly Ile Pro Ile Ile Lys Ala Gly Thr Val Ile Lys Leu 
                85                  90                  95      
Ile Glu Arg Leu Thr Tyr His Met Tyr Ala Asp Pro Asn Phe Val Arg 
            100                 105                 110         
Thr Phe Leu Thr Thr Tyr Arg Ser Phe Cys Lys Pro Gln Glu Leu Leu 
        115                 120                 125             
Ser Leu Ile Ile Glu Arg Phe Glu Ile Pro Glu Pro Glu Pro Thr Glu 
    130                 135                 140                 
Ala Asp Arg Ile Ala Ile Glu Asn Gly Asp Gln Pro Leu Ser Ala Glu 
145                 150                 155                 160 
Leu Lys Arg Phe Arg Lys Glu Tyr Ile Gln Pro Val Gln Leu Arg Val 
                165                 170                 175     
Leu Asn Val Cys Arg His Trp Val Glu His His Phe Tyr Asp Phe Glu 
            180                 185                 190         
Arg Asp Ala Tyr Leu Leu Gln Arg Met Glu Glu Phe Ile Gly Thr Val 
        195                 200                 205             
Arg Gly Lys Ala Met Lys Lys Trp Val Glu Ser Ile Thr Lys Ile Ile 
    210                 215                 220                 
Gln Arg Lys Lys Ile Ala Arg Asp Asn Gly Pro Gly His Asn Ile Thr 
225                 230                 235                 240 
Phe Gln Ser Ser Pro Pro Thr Val Glu Trp His Ile Ser Arg Pro Gly 
                245                 250                 255     
His Ile Glu Thr Phe Asp Leu Leu Thr Leu His Pro Ile Glu Ile Ala 
            260                 265                 270         
Arg Gln Leu Thr Leu Leu Glu Ser Asp Leu Tyr Arg Ala Val Gln Pro 
        275                 280                 285             
Ser Glu Leu Val Gly Ser Val Trp Thr Lys Glu Asp Lys Glu Ile Asn 
    290                 295                 300                 
Ser Pro Asn Leu Leu Lys Met Ile Arg His Thr Thr Asn Leu Thr Leu 
305                 310                 315                 320 
Trp Phe Glu Lys Cys Ile Val Glu Thr Glu Asn Leu Glu Glu Arg Val 
                325                 330                 335     
Ala Val Val Ser Arg Ile Ile Glu Ile Leu Gln Val Phe Gln Glu Leu 
            340                 345                 350         
Asn Asn Phe Asn Gly Val Leu Glu Val Val Ser Ala Met Asn Ser Ser 
        355                 360                 365             
Pro Val Tyr Arg Leu Asp His Thr Phe Glu Gln Ile Pro Ser Arg Gln 
    370                 375                 380                 
Lys Lys Ile Leu Glu Glu Ala His Glu Leu Ser Glu Asp His Tyr Lys 
385                 390                 395                 400 
Lys Tyr Leu Ala Lys Leu Arg Ser Ile Asn Pro Pro Cys Val Pro Phe 
                405                 410                 415     
Phe Gly Ile Tyr Leu Thr Asn Ile Leu Lys Thr Glu Glu Gly Asn Pro 
            420                 425                 430         
Glu Val Leu Lys Arg His Gly Lys Glu Leu Ile Asn Phe Ser Lys Arg 
        435                 440                 445             
Arg Lys Val Ala Glu Ile Thr Gly Glu Ile Gln Gln Tyr Gln Asn Gln 
    450                 455                 460                 
Pro Tyr Cys Leu Arg Val Glu Ser Asp Ile Lys Arg Phe Phe Glu Asn 
465                 470                 475                 480 
Leu Asn Pro Met Gly Asn Ser Met Glu Lys Glu Phe Thr Asp Tyr Leu 
                485                 490                 495     
Phe Asn Lys Ser Leu Glu Ile Glu Pro Arg Asn Pro Lys Pro Leu Pro 
            500                 505                 510         
Arg Phe Pro Lys Lys Tyr Ser Tyr Pro Leu Lys Ser Pro Gly Val Arg 
        515                 520                 525             
Pro Ser Asn Pro Arg Pro Gly Thr 
    530                 535     

<210> 4
<211> 490
<212> PRT
<213> synthetic organisms


<220> 
<223> hSOS1_12 (tag-free)

<400> 4
Gly Ala Met Ala Glu Glu Gln Met Arg Leu Pro Ser Ala Asp Val Tyr 
1               5                   10                  15      
Arg Phe Ala Glu Pro Asp Ser Glu Glu Asn Ile Ile Phe Glu Glu Asn 
            20                  25                  30          
Met Gln Pro Lys Ala Gly Ile Pro Ile Ile Lys Ala Gly Thr Val Ile 
        35                  40                  45              
Lys Leu Ile Glu Arg Leu Thr Tyr His Met Tyr Ala Asp Pro Asn Phe 
    50                  55                  60                  
Val Arg Thr Phe Leu Thr Thr Tyr Arg Ser Phe Cys Lys Pro Gln Glu 
65                  70                  75                  80  
Leu Leu Ser Leu Ile Ile Glu Arg Phe Glu Ile Pro Glu Pro Glu Pro 
                85                  90                  95      
Thr Glu Ala Asp Arg Ile Ala Ile Glu Asn Gly Asp Gln Pro Leu Ser 
            100                 105                 110         
Ala Glu Leu Lys Arg Phe Arg Lys Glu Tyr Ile Gln Pro Val Gln Leu 
        115                 120                 125             
Arg Val Leu Asn Val Cys Arg His Trp Val Glu His His Phe Tyr Asp 
    130                 135                 140                 
Phe Glu Arg Asp Ala Tyr Leu Leu Gln Arg Met Glu Glu Phe Ile Gly 
145                 150                 155                 160 
Thr Val Arg Gly Lys Ala Met Lys Lys Trp Val Glu Ser Ile Thr Lys 
                165                 170                 175     
Ile Ile Gln Arg Lys Lys Ile Ala Arg Asp Asn Gly Pro Gly His Asn 
            180                 185                 190         
Ile Thr Phe Gln Ser Ser Pro Pro Thr Val Glu Trp His Ile Ser Arg 
        195                 200                 205             
Pro Gly His Ile Glu Thr Phe Asp Leu Leu Thr Leu His Pro Ile Glu 
    210                 215                 220                 
Ile Ala Arg Gln Leu Thr Leu Leu Glu Ser Asp Leu Tyr Arg Ala Val 
225                 230                 235                 240 
Gln Pro Ser Glu Leu Val Gly Ser Val Trp Thr Lys Glu Asp Lys Glu 
                245                 250                 255     
Ile Asn Ser Pro Asn Leu Leu Lys Met Ile Arg His Thr Thr Asn Leu 
            260                 265                 270         
Thr Leu Trp Phe Glu Lys Cys Ile Val Glu Thr Glu Asn Leu Glu Glu 
        275                 280                 285             
Arg Val Ala Val Val Ser Arg Ile Ile Glu Ile Leu Gln Val Phe Gln 
    290                 295                 300                 
Glu Leu Asn Asn Phe Asn Gly Val Leu Glu Val Val Ser Ala Met Asn 
305                 310                 315                 320 
Ser Ser Pro Val Tyr Arg Leu Asp His Thr Phe Glu Gln Ile Pro Ser 
                325                 330                 335     
Arg Gln Lys Lys Ile Leu Glu Glu Ala His Glu Leu Ser Glu Asp His 
            340                 345                 350         
Tyr Lys Lys Tyr Leu Ala Lys Leu Arg Ser Ile Asn Pro Pro Cys Val 
        355                 360                 365             
Pro Phe Phe Gly Ile Tyr Leu Thr Asn Ile Leu Lys Thr Glu Glu Gly 
    370                 375                 380                 
Asn Pro Glu Val Leu Lys Arg His Gly Lys Glu Leu Ile Asn Phe Ser 
385                 390                 395                 400 
Lys Arg Arg Lys Val Ala Glu Ile Thr Gly Glu Ile Gln Gln Tyr Gln 
                405                 410                 415     
Asn Gln Pro Tyr Cys Leu Arg Val Glu Ser Asp Ile Lys Arg Phe Phe 
            420                 425                 430         
Glu Asn Leu Asn Pro Met Gly Asn Ser Met Glu Lys Glu Phe Thr Asp 
        435                 440                 445             
Tyr Leu Phe Asn Lys Ser Leu Glu Ile Glu Pro Arg Asn Pro Lys Pro 
    450                 455                 460                 
Leu Pro Arg Phe Pro Lys Lys Tyr Ser Tyr Pro Leu Lys Ser Pro Gly 
465                 470                 475                 480 
Val Arg Pro Ser Asn Pro Arg Pro Gly Thr 
                485                 490 

<210> 5
<211> 527
<212> PRT
<213> synthetic organisms


<220> 
<223> His10-hSOS2

<400> 5
Met Gly His His His His His His His His His His Ser Ser Gly His 
1               5                   10                  15      
Ile Glu Gly Arg His Met Leu Glu Thr Ser Leu Tyr Lys Lys Ala Gly 
            20                  25                  30          
Ser Asp Tyr Asp Ile Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Pro 
        35                  40                  45              
Leu Arg Leu Pro Ser Pro Glu Val Tyr Arg Phe Val Val Lys Asp Ser 
    50                  55                  60                  
Glu Glu Asn Ile Val Phe Glu Asp Asn Leu Gln Ser Arg Ser Gly Ile 
65                  70                  75                  80  
Pro Ile Ile Lys Gly Gly Thr Val Val Lys Leu Ile Glu Arg Leu Thr 
                85                  90                  95      
Tyr His Met Tyr Ala Asp Pro Asn Phe Val Arg Thr Phe Leu Thr Thr 
            100                 105                 110         
Tyr Arg Ser Phe Cys Lys Pro Gln Glu Leu Leu Ser Leu Leu Ile Glu 
        115                 120                 125             
Arg Phe Glu Ile Pro Glu Pro Glu Pro Thr Asp Ala Asp Lys Leu Ala 
    130                 135                 140                 
Ile Glu Lys Gly Glu Gln Pro Ile Ser Ala Asp Leu Lys Arg Phe Arg 
145                 150                 155                 160 
Lys Glu Tyr Val Gln Pro Val Gln Leu Arg Ile Leu Asn Val Phe Arg 
                165                 170                 175     
His Trp Val Glu His His Phe Tyr Asp Phe Glu Arg Asp Leu Glu Leu 
            180                 185                 190         
Leu Glu Arg Leu Glu Ser Phe Ile Ser Ser Val Arg Gly Lys Ala Met 
        195                 200                 205             
Lys Lys Trp Val Glu Ser Ile Ala Lys Ile Ile Arg Arg Lys Lys Gln 
    210                 215                 220                 
Ala Gln Ala Asn Gly Val Ser His Asn Ile Thr Phe Glu Ser Pro Pro 
225                 230                 235                 240 
Pro Pro Ile Glu Trp His Ile Ser Lys Pro Gly Gln Phe Glu Thr Phe 
                245                 250                 255     
Asp Leu Met Thr Leu His Pro Ile Glu Ile Ala Arg Gln Leu Thr Leu 
            260                 265                 270         
Leu Glu Ser Asp Leu Tyr Arg Lys Val Gln Pro Ser Glu Leu Val Gly 
        275                 280                 285             
Ser Val Trp Thr Lys Glu Asp Lys Glu Ile Asn Ser Pro Asn Leu Leu 
    290                 295                 300                 
Lys Met Ile Arg His Thr Thr Asn Leu Thr Leu Trp Phe Glu Lys Cys 
305                 310                 315                 320 
Ile Val Glu Ala Glu Asn Phe Glu Glu Arg Val Ala Val Leu Ser Arg 
                325                 330                 335     
Ile Ile Glu Ile Leu Gln Val Phe Gln Asp Leu Asn Asn Phe Asn Gly 
            340                 345                 350         
Val Leu Glu Ile Val Ser Ala Val Asn Ser Val Ser Val Tyr Arg Leu 
        355                 360                 365             
Asp His Thr Phe Glu Ala Leu Gln Glu Arg Lys Arg Lys Ile Leu Asp 
    370                 375                 380                 
Glu Ala Val Glu Leu Ser Gln Asp His Phe Lys Lys Tyr Leu Val Lys 
385                 390                 395                 400 
Leu Lys Ser Ile Asn Pro Pro Cys Val Pro Phe Phe Gly Ile Tyr Leu 
                405                 410                 415     
Thr Asn Ile Leu Lys Thr Glu Glu Gly Asn Asn Asp Phe Leu Lys Lys 
            420                 425                 430         
Lys Gly Lys Asp Leu Ile Asn Phe Ser Lys Arg Arg Lys Val Ala Glu 
        435                 440                 445             
Ile Thr Gly Glu Ile Gln Gln Tyr Gln Asn Gln Pro Tyr Cys Leu Arg 
    450                 455                 460                 
Ile Glu Pro Asp Met Arg Arg Phe Phe Glu Asn Leu Asn Pro Met Gly 
465                 470                 475                 480 
Ser Ala Ser Glu Lys Glu Phe Thr Asp Tyr Leu Phe Asn Lys Ser Leu 
                485                 490                 495     
Glu Ile Glu Pro Arg Asn Cys Lys Gln Pro Pro Arg Phe Pro Arg Lys 
            500                 505                 510         
Ser Thr Phe Ser Leu Lys Ser Pro Gly Ile Arg Pro Asn Thr Gly 
        515                 520                 525         

<210> 6
<211> 536
<212> PRT
<213> synthetic organisms


<220> 
<223> hSOS1_12 with N-terminal His tag (His10-hSOS1_12) before cleavage
      by TEV protease

<400> 6
Met Gly His His His His His His His His His His Ser Ser Gly His 
1               5                   10                  15      
Ile Glu Gly Arg His Met Leu Glu Thr Ser Leu Tyr Lys Lys Ala Gly 
            20                  25                  30          
Ser Asp Tyr Asp Ile Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Ala 
        35                  40                  45              
Met Ala Glu Glu Gln Met Arg Leu Pro Ser Ala Asp Val Tyr Arg Phe 
    50                  55                  60                  
Ala Glu Pro Asp Ser Glu Glu Asn Ile Ile Phe Glu Glu Asn Met Gln 
65                  70                  75                  80  
Pro Lys Ala Gly Ile Pro Ile Ile Lys Ala Gly Thr Val Ile Lys Leu 
                85                  90                  95      
Ile Glu Arg Leu Thr Tyr His Met Tyr Ala Asp Pro Asn Phe Val Arg 
            100                 105                 110         
Thr Phe Leu Thr Thr Tyr Arg Ser Phe Cys Lys Pro Gln Glu Leu Leu 
        115                 120                 125             
Ser Leu Ile Ile Glu Arg Phe Glu Ile Pro Glu Pro Glu Pro Thr Glu 
    130                 135                 140                 
Ala Asp Arg Ile Ala Ile Glu Asn Gly Asp Gln Pro Leu Ser Ala Glu 
145                 150                 155                 160 
Leu Lys Arg Phe Arg Lys Glu Tyr Ile Gln Pro Val Gln Leu Arg Val 
                165                 170                 175     
Leu Asn Val Cys Arg His Trp Val Glu His His Phe Tyr Asp Phe Glu 
            180                 185                 190         
Arg Asp Ala Tyr Leu Leu Gln Arg Met Glu Glu Phe Ile Gly Thr Val 
        195                 200                 205             
Arg Gly Lys Ala Met Lys Lys Trp Val Glu Ser Ile Thr Lys Ile Ile 
    210                 215                 220                 
Gln Arg Lys Lys Ile Ala Arg Asp Asn Gly Pro Gly His Asn Ile Thr 
225                 230                 235                 240 
Phe Gln Ser Ser Pro Pro Thr Val Glu Trp His Ile Ser Arg Pro Gly 
                245                 250                 255     
His Ile Glu Thr Phe Asp Leu Leu Thr Leu His Pro Ile Glu Ile Ala 
            260                 265                 270         
Arg Gln Leu Thr Leu Leu Glu Ser Asp Leu Tyr Arg Ala Val Gln Pro 
        275                 280                 285             
Ser Glu Leu Val Gly Ser Val Trp Thr Lys Glu Asp Lys Glu Ile Asn 
    290                 295                 300                 
Ser Pro Asn Leu Leu Lys Met Ile Arg His Thr Thr Asn Leu Thr Leu 
305                 310                 315                 320 
Trp Phe Glu Lys Cys Ile Val Glu Thr Glu Asn Leu Glu Glu Arg Val 
                325                 330                 335     
Ala Val Val Ser Arg Ile Ile Glu Ile Leu Gln Val Phe Gln Glu Leu 
            340                 345                 350         
Asn Asn Phe Asn Gly Val Leu Glu Val Val Ser Ala Met Asn Ser Ser 
        355                 360                 365             
Pro Val Tyr Arg Leu Asp His Thr Phe Glu Gln Ile Pro Ser Arg Gln 
    370                 375                 380                 
Lys Lys Ile Leu Glu Glu Ala His Glu Leu Ser Glu Asp His Tyr Lys 
385                 390                 395                 400 
Lys Tyr Leu Ala Lys Leu Arg Ser Ile Asn Pro Pro Cys Val Pro Phe 
                405                 410                 415     
Phe Gly Ile Tyr Leu Thr Asn Ile Leu Lys Thr Glu Glu Gly Asn Pro 
            420                 425                 430         
Glu Val Leu Lys Arg His Gly Lys Glu Leu Ile Asn Phe Ser Lys Arg 
        435                 440                 445             
Arg Lys Val Ala Glu Ile Thr Gly Glu Ile Gln Gln Tyr Gln Asn Gln 
    450                 455                 460                 
Pro Tyr Cys Leu Arg Val Glu Ser Asp Ile Lys Arg Phe Phe Glu Asn 
465                 470                 475                 480 
Leu Asn Pro Met Gly Asn Ser Met Glu Lys Glu Phe Thr Asp Tyr Leu 
                485                 490                 495     
Phe Asn Lys Ser Leu Glu Ile Glu Pro Arg Asn Pro Lys Pro Leu Pro 
            500                 505                 510         
Arg Phe Pro Lys Lys Tyr Ser Tyr Pro Leu Lys Ser Pro Gly Val Arg 
        515                 520                 525             
Pro Ser Asn Pro Arg Pro Gly Thr 
    530                 535     

<210> 7
<211> 490
<212> PRT
<213> synthetic organisms


<220> 
<223> hSOS1_12 after cleavage by TEV protease

<400> 7
Gly Ala Met Ala Glu Glu Gln Met Arg Leu Pro Ser Ala Asp Val Tyr 
1               5                   10                  15      
Arg Phe Ala Glu Pro Asp Ser Glu Glu Asn Ile Ile Phe Glu Glu Asn 
            20                  25                  30          
Met Gln Pro Lys Ala Gly Ile Pro Ile Ile Lys Ala Gly Thr Val Ile 
        35                  40                  45              
Lys Leu Ile Glu Arg Leu Thr Tyr His Met Tyr Ala Asp Pro Asn Phe 
    50                  55                  60                  
Val Arg Thr Phe Leu Thr Thr Tyr Arg Ser Phe Cys Lys Pro Gln Glu 
65                  70                  75                  80  
Leu Leu Ser Leu Ile Ile Glu Arg Phe Glu Ile Pro Glu Pro Glu Pro 
                85                  90                  95      
Thr Glu Ala Asp Arg Ile Ala Ile Glu Asn Gly Asp Gln Pro Leu Ser 
            100                 105                 110         
Ala Glu Leu Lys Arg Phe Arg Lys Glu Tyr Ile Gln Pro Val Gln Leu 
        115                 120                 125             
Arg Val Leu Asn Val Cys Arg His Trp Val Glu His His Phe Tyr Asp 
    130                 135                 140                 
Phe Glu Arg Asp Ala Tyr Leu Leu Gln Arg Met Glu Glu Phe Ile Gly 
145                 150                 155                 160 
Thr Val Arg Gly Lys Ala Met Lys Lys Trp Val Glu Ser Ile Thr Lys 
                165                 170                 175     
Ile Ile Gln Arg Lys Lys Ile Ala Arg Asp Asn Gly Pro Gly His Asn 
            180                 185                 190         
Ile Thr Phe Gln Ser Ser Pro Pro Thr Val Glu Trp His Ile Ser Arg 
        195                 200                 205             
Pro Gly His Ile Glu Thr Phe Asp Leu Leu Thr Leu His Pro Ile Glu 
    210                 215                 220                 
Ile Ala Arg Gln Leu Thr Leu Leu Glu Ser Asp Leu Tyr Arg Ala Val 
225                 230                 235                 240 
Gln Pro Ser Glu Leu Val Gly Ser Val Trp Thr Lys Glu Asp Lys Glu 
                245                 250                 255     
Ile Asn Ser Pro Asn Leu Leu Lys Met Ile Arg His Thr Thr Asn Leu 
            260                 265                 270         
Thr Leu Trp Phe Glu Lys Cys Ile Val Glu Thr Glu Asn Leu Glu Glu 
        275                 280                 285             
Arg Val Ala Val Val Ser Arg Ile Ile Glu Ile Leu Gln Val Phe Gln 
    290                 295                 300                 
Glu Leu Asn Asn Phe Asn Gly Val Leu Glu Val Val Ser Ala Met Asn 
305                 310                 315                 320 
Ser Ser Pro Val Tyr Arg Leu Asp His Thr Phe Glu Gln Ile Pro Ser 
                325                 330                 335     
Arg Gln Lys Lys Ile Leu Glu Glu Ala His Glu Leu Ser Glu Asp His 
            340                 345                 350         
Tyr Lys Lys Tyr Leu Ala Lys Leu Arg Ser Ile Asn Pro Pro Cys Val 
        355                 360                 365             
Pro Phe Phe Gly Ile Tyr Leu Thr Asn Ile Leu Lys Thr Glu Glu Gly 
    370                 375                 380                 
Asn Pro Glu Val Leu Lys Arg His Gly Lys Glu Leu Ile Asn Phe Ser 
385                 390                 395                 400 
Lys Arg Arg Lys Val Ala Glu Ile Thr Gly Glu Ile Gln Gln Tyr Gln 
                405                 410                 415     
Asn Gln Pro Tyr Cys Leu Arg Val Glu Ser Asp Ile Lys Arg Phe Phe 
            420                 425                 430         
Glu Asn Leu Asn Pro Met Gly Asn Ser Met Glu Lys Glu Phe Thr Asp 
        435                 440                 445             
Tyr Leu Phe Asn Lys Ser Leu Glu Ile Glu Pro Arg Asn Pro Lys Pro 
    450                 455                 460                 
Leu Pro Arg Phe Pro Lys Lys Tyr Ser Tyr Pro Leu Lys Ser Pro Gly 
465                 470                 475                 480 
Val Arg Pro Ser Asn Pro Arg Pro Gly Thr 
                485                 490 

