                         SEQUENCE LISTING

<110>  йũҵѧ
 
<120>  һCRISPR/CasЧӦ׼ϵͳ

<130>  IEC180036PCT

<150>  CN 201810360287.7
<151>  2018-04-20

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  1093
<212>  PRT
<213>  ˹

<220>
<223>  Cas12i.1İ

<400>  1

Met Ser Asn Lys Glu Lys Asn Ala Ser Glu Thr Arg Lys Ala Tyr Thr 
1               5                   10                  15      


Thr Lys Met Ile Pro Arg Ser His Asp Arg Met Lys Leu Leu Gly Asn 
            20                  25                  30          


Phe Met Asp Tyr Leu Met Asp Gly Thr Pro Ile Phe Phe Glu Leu Trp 
        35                  40                  45              


Asn Gln Phe Gly Gly Gly Ile Asp Arg Asp Ile Ile Ser Gly Thr Ala 
    50                  55                  60                  


Asn Lys Asp Lys Ile Ser Asp Asp Leu Leu Leu Ala Val Asn Trp Phe 
65                  70                  75                  80  


Lys Val Met Pro Ile Asn Ser Lys Pro Gln Gly Val Ser Pro Ser Asn 
                85                  90                  95      


Leu Ala Asn Leu Phe Gln Gln Tyr Ser Gly Ser Glu Pro Asp Ile Gln 
            100                 105                 110         


Ala Gln Glu Tyr Phe Ala Ser Asn Phe Asp Thr Glu Lys His Gln Trp 
        115                 120                 125             


Lys Asp Met Arg Val Glu Tyr Glu Arg Leu Leu Ala Glu Leu Gln Leu 
    130                 135                 140                 


Ser Arg Ser Asp Met His His Asp Leu Lys Leu Met Tyr Lys Glu Lys 
145                 150                 155                 160 


Cys Ile Gly Leu Ser Leu Ser Thr Ala His Tyr Ile Thr Ser Val Met 
                165                 170                 175     


Phe Gly Thr Gly Ala Lys Asn Asn Arg Gln Thr Lys His Gln Phe Tyr 
            180                 185                 190         


Ser Lys Val Ile Gln Leu Leu Glu Glu Ser Thr Gln Ile Asn Ser Val 
        195                 200                 205             


Glu Gln Leu Ala Ser Ile Ile Leu Lys Ala Gly Asp Cys Asp Ser Tyr 
    210                 215                 220                 


Arg Lys Leu Arg Ile Arg Cys Ser Arg Lys Gly Ala Thr Pro Ser Ile 
225                 230                 235                 240 


Leu Lys Ile Val Gln Asp Tyr Glu Leu Gly Thr Asn His Asp Asp Glu 
                245                 250                 255     


Val Asn Val Pro Ser Leu Ile Ala Asn Leu Lys Glu Lys Leu Gly Arg 
            260                 265                 270         


Phe Glu Tyr Glu Cys Glu Trp Lys Cys Met Glu Lys Ile Lys Ala Phe 
        275                 280                 285             


Leu Ala Ser Lys Val Gly Pro Tyr Tyr Leu Gly Ser Tyr Ser Ala Met 
    290                 295                 300                 


Leu Glu Asn Ala Leu Ser Pro Ile Lys Gly Met Thr Thr Lys Asn Cys 
305                 310                 315                 320 


Lys Phe Val Leu Lys Gln Ile Asp Ala Lys Asn Asp Ile Lys Tyr Glu 
                325                 330                 335     


Asn Glu Pro Phe Gly Lys Ile Val Glu Gly Phe Phe Asp Ser Pro Tyr 
            340                 345                 350         


Phe Glu Ser Asp Thr Asn Val Lys Trp Val Leu His Pro His His Ile 
        355                 360                 365             


Gly Glu Ser Asn Ile Lys Thr Leu Trp Glu Asp Leu Asn Ala Ile His 
    370                 375                 380                 


Ser Lys Tyr Glu Glu Asp Ile Ala Ser Leu Ser Glu Asp Lys Lys Glu 
385                 390                 395                 400 


Lys Arg Ile Lys Val Tyr Gln Gly Asp Val Cys Gln Thr Ile Asn Thr 
                405                 410                 415     


Tyr Cys Glu Glu Val Gly Lys Glu Ala Lys Thr Pro Leu Val Gln Leu 
            420                 425                 430         


Leu Arg Tyr Leu Tyr Ser Arg Lys Asp Asp Ile Ala Val Asp Lys Ile 
        435                 440                 445             


Ile Asp Gly Ile Thr Phe Leu Ser Lys Lys His Lys Val Glu Lys Gln 
    450                 455                 460                 


Lys Ile Asn Pro Val Ile Gln Lys Tyr Pro Ser Phe Asn Phe Gly Asn 
465                 470                 475                 480 


Asn Ser Lys Leu Leu Gly Lys Ile Ile Ser Pro Lys Asp Lys Leu Lys 
                485                 490                 495     


His Asn Leu Lys Cys Asn Arg Asn Gln Val Asp Asn Tyr Ile Trp Ile 
            500                 505                 510         


Glu Ile Lys Val Leu Asn Thr Lys Thr Met Arg Trp Glu Lys His His 
        515                 520                 525             


Tyr Ala Leu Ser Ser Thr Arg Phe Leu Glu Glu Val Tyr Tyr Pro Ala 
    530                 535                 540                 


Thr Ser Glu Asn Pro Pro Asp Ala Leu Ala Ala Arg Phe Arg Thr Lys 
545                 550                 555                 560 


Thr Asn Gly Tyr Glu Gly Lys Pro Ala Leu Ser Ala Glu Gln Ile Glu 
                565                 570                 575     


Gln Ile Arg Ser Ala Pro Val Gly Leu Arg Lys Val Lys Lys Arg Gln 
            580                 585                 590         


Met Arg Leu Glu Ala Ala Arg Gln Gln Asn Leu Leu Pro Arg Tyr Thr 
        595                 600                 605             


Trp Gly Lys Asp Phe Asn Ile Asn Ile Cys Lys Arg Gly Asn Asn Phe 
    610                 615                 620                 


Glu Val Thr Leu Ala Thr Lys Val Lys Lys Lys Lys Glu Lys Asn Tyr 
625                 630                 635                 640 


Lys Val Val Leu Gly Tyr Asp Ala Asn Ile Val Arg Lys Asn Thr Tyr 
                645                 650                 655     


Ala Ala Ile Glu Ala His Ala Asn Gly Asp Gly Val Ile Asp Tyr Asn 
            660                 665                 670         


Asp Leu Pro Val Lys Pro Ile Glu Ser Gly Phe Val Thr Val Glu Ser 
        675                 680                 685             


Gln Val Arg Asp Lys Ser Tyr Asp Gln Leu Ser Tyr Asn Gly Val Lys 
    690                 695                 700                 


Leu Leu Tyr Cys Lys Pro His Val Glu Ser Arg Arg Ser Phe Leu Glu 
705                 710                 715                 720 


Lys Tyr Arg Asn Gly Thr Met Lys Asp Asn Arg Gly Asn Asn Ile Gln 
                725                 730                 735     


Ile Asp Phe Met Lys Asp Phe Glu Ala Ile Ala Asp Asp Glu Thr Ser 
            740                 745                 750         


Leu Tyr Tyr Phe Asn Met Lys Tyr Cys Lys Leu Leu Gln Ser Ser Ile 
        755                 760                 765             


Arg Asn His Ser Ser Gln Ala Lys Glu Tyr Arg Glu Glu Ile Phe Glu 
    770                 775                 780                 


Leu Leu Arg Asp Gly Lys Leu Ser Val Leu Lys Leu Ser Ser Leu Ser 
785                 790                 795                 800 


Asn Leu Ser Phe Val Met Phe Lys Val Ala Lys Ser Leu Ile Gly Thr 
                805                 810                 815     


Tyr Phe Gly His Leu Leu Lys Lys Pro Lys Asn Ser Lys Ser Asp Val 
            820                 825                 830         


Lys Ala Pro Pro Ile Thr Asp Glu Asp Lys Gln Lys Ala Asp Pro Glu 
        835                 840                 845             


Met Phe Ala Leu Arg Leu Ala Leu Glu Glu Lys Arg Leu Asn Lys Val 
    850                 855                 860                 


Lys Ser Lys Lys Glu Val Ile Ala Asn Lys Ile Val Ala Lys Ala Leu 
865                 870                 875                 880 


Glu Leu Arg Asp Lys Tyr Gly Pro Val Leu Ile Lys Gly Glu Asn Ile 
                885                 890                 895     


Ser Asp Thr Thr Lys Lys Gly Lys Lys Ser Ser Thr Asn Ser Phe Leu 
            900                 905                 910         


Met Asp Trp Leu Ala Arg Gly Val Ala Asn Lys Val Lys Glu Met Val 
        915                 920                 925             


Met Met His Gln Gly Leu Glu Phe Val Glu Val Asn Pro Asn Phe Thr 
    930                 935                 940                 


Ser His Gln Asp Pro Phe Val His Lys Asn Pro Glu Asn Thr Phe Arg 
945                 950                 955                 960 


Ala Arg Tyr Ser Arg Cys Thr Pro Ser Glu Leu Thr Glu Lys Asn Arg 
                965                 970                 975     


Lys Glu Ile Leu Ser Phe Leu Ser Asp Lys Pro Ser Lys Arg Pro Thr 
            980                 985                 990         


Asn Ala Tyr Tyr Asn Glu Gly Ala  Met Ala Phe Leu Ala  Thr Tyr Gly 
        995                 1000                 1005             


Leu Lys  Lys Asn Asp Val Leu  Gly Val Ser Leu Glu  Lys Phe Lys 
    1010                 1015                 1020             


Gln Ile  Met Ala Asn Ile Leu  His Gln Arg Ser Glu  Asp Gln Leu 
    1025                 1030                 1035             


Leu Phe  Pro Ser Arg Gly Gly  Met Phe Tyr Leu Ala  Thr Tyr Lys 
    1040                 1045                 1050             


Leu Asp  Ala Asp Ala Thr Ser  Val Asn Trp Asn Gly  Lys Gln Phe 
    1055                 1060                 1065             


Trp Val  Cys Asn Ala Asp Leu  Val Ala Ala Tyr Asn  Val Gly Leu 
    1070                 1075                 1080             


Val Asp  Ile Gln Lys Asp Phe  Lys Lys Lys 
    1085                 1090             


<210>  2
<211>  1046
<212>  PRT
<213>  ˹

<220>
<223>  Cas12i.2İ

<400>  2

Met Val Ser Glu Ser Thr Ile Arg Pro Tyr Thr Ser Lys Leu Ala Pro 
1               5                   10                  15      


Asn Asp Ser Lys Leu Lys Met Leu Asn Asp Thr Phe Asn Trp Leu Asp 
            20                  25                  30          


His Ala Tyr Lys Val Phe Phe Asp Val Ser Val Ala Leu Phe Gly Ala 
        35                  40                  45              


Ile Glu His Glu Thr Ala Gln Glu Leu Ile Gly Glu Lys Ser Lys Phe 
    50                  55                  60                  


Asp Ala Asp Leu Leu Cys Ala Ile Met Trp Phe Arg Leu Glu Glu Lys 
65                  70                  75                  80  


Ser Asp Asn Pro Gly Pro Leu Gln Thr Val Glu Gln Arg Met Arg Leu 
                85                  90                  95      


Phe Gln Lys Tyr Ser Gly His Glu Pro Ser Ser Phe Thr Gln Glu Tyr 
            100                 105                 110         


Ile Lys Gly Asn Ile Asp Ser Glu Lys Tyr Gln Trp Val Asp Cys Arg 
        115                 120                 125             


Leu Lys Phe Ile Asp Leu Ala Arg Asn Ile Asn Thr Thr Gln Glu Ser 
    130                 135                 140                 


Leu Lys Ile Asp Ala Tyr Thr Leu Phe Met Asn Lys Leu Ile Pro Val 
145                 150                 155                 160 


Ser Lys Asp Asp Glu Phe Asn Ala Tyr Gly Leu Ile Ser Gln Leu Phe 
                165                 170                 175     


Gly Thr Gly Lys Lys Glu Asp Arg Ser Ile Lys Ala Ser Met Leu Glu 
            180                 185                 190         


Glu Ile Ser Asn Ile Leu Ala Asp Lys Asn Pro Asn Thr Trp Glu Glu 
        195                 200                 205             


Tyr Gln Asp Leu Ile Lys Lys Thr Phe Asn Val Asp Asn Tyr Lys Glu 
    210                 215                 220                 


Leu Lys Glu Lys Leu Ser Ala Gly Ser Ser Gly Arg Asp Gly Ser Leu 
225                 230                 235                 240 


Val Ile Asp Leu Lys Glu Glu Lys Thr Gly Leu Leu Gln Pro Asn Phe 
                245                 250                 255     


Ile Lys Asn Arg Ile Val Lys Phe Arg Glu Asp Ala Asp Lys Lys Arg 
            260                 265                 270         


Thr Val Phe Leu Leu Pro Asn Arg Met Lys Leu Arg Glu Phe Ile Ala 
        275                 280                 285             


Ser Gln Ile Gly Pro Phe Glu Gln Asn Ser Trp Ser Ala Val Leu Asn 
    290                 295                 300                 


Arg Ser Met Ala Ala Ile Gln Ser Lys Asn Ser Ser Asn Ile Leu Tyr 
305                 310                 315                 320 


Thr Asn Glu Lys Glu Glu Arg Asn Asn Glu Ile Gln Glu Leu Leu Lys 
                325                 330                 335     


Lys Asp Ile Leu Ser Ala Ala Ser Ile Leu Gly Asp Phe Arg Arg Gly 
            340                 345                 350         


Glu Phe Asn Arg Ser Val Val Ser Lys Asn His Leu Gly Ala Arg Leu 
        355                 360                 365             


Asn Glu Leu Phe Glu Ile Trp Gln Glu Leu Thr Met Asp Asp Gly Ile 
    370                 375                 380                 


Lys Lys Tyr Val Asp Leu Cys Lys Asp Lys Phe Ser Arg Arg Pro Val 
385                 390                 395                 400 


Lys Ala Leu Leu Gln Tyr Ile Tyr Pro Tyr Phe Asp Lys Ile Asn Ala 
                405                 410                 415     


Lys Gln Phe Leu Asp Ala Ala Ser Tyr Asn Thr Leu Val Glu Thr Asn 
            420                 425                 430         


Asn Arg Lys Lys Ile His Pro Thr Val Thr Gly Pro Thr Val Cys Asn 
        435                 440                 445             


Trp Gly Pro Lys Ser Thr Ile Asn Gly Ser Ile Thr Pro Pro Asn Gln 
    450                 455                 460                 


Met Val Lys Gly Arg Pro Ala Gly Ser His Gly Met Ile Trp Val Thr 
465                 470                 475                 480 


Met Thr Val Ile Asp Asn Gly Arg Trp Ile Lys His His Leu Pro Phe 
                485                 490                 495     


His Asn Ser Arg Tyr Tyr Glu Glu His Tyr Cys Tyr Arg Glu Gly Leu 
            500                 505                 510         


Pro Thr Lys Asn Lys Pro Arg Thr Lys Gln Leu Gly Thr Gln Val Gly 
        515                 520                 525             


Ser Thr Ile Ser Ala Pro Ser Leu Ala Ile Leu Lys Ser Gln Glu Glu 
    530                 535                 540                 


Gln Asp Arg Arg Asn Asp Arg Lys Asn Arg Phe Lys Ala His Lys Ser 
545                 550                 555                 560 


Ile Ile Arg Ser Gln Glu Asn Ile Glu Tyr Asn Val Ala Phe Asp Lys 
                565                 570                 575     


Ser Thr Asn Phe Asp Val Thr Arg Lys Asn Gly Glu Phe Phe Ile Thr 
            580                 585                 590         


Ile Ser Ser Arg Val Ala Thr Pro Lys Tyr Ser Tyr Lys Leu Asn Ile 
        595                 600                 605             


Gly Asp Met Ile Met Gly Leu Asp Asn Asn Gln Thr Ala Pro Cys Thr 
    610                 615                 620                 


Tyr Ser Ile Trp Arg Val Val Glu Lys Asp Thr Glu Gly Ser Phe Phe 
625                 630                 635                 640 


His Asn Lys Ile Trp Leu Gln Leu Val Thr Asp Gly Lys Val Thr Ser 
                645                 650                 655     


Ile Val Asp Asn Asn Arg Gln Val Asp Gln Leu Ser Tyr Ala Gly Ile 
            660                 665                 670         


Glu Tyr Ser Asn Phe Ala Glu Trp Arg Lys Asp Arg Arg Gln Phe Leu 
        675                 680                 685             


Arg Ser Ile Asn Glu Asp Tyr Val Lys Lys Ser Asp Asn Trp Arg Asn 
    690                 695                 700                 


Met Asn Leu Tyr Gln Trp Asn Ala Glu Tyr Ser Arg Leu Leu Leu Asp 
705                 710                 715                 720 


Val Met Lys Glu Asn Lys Gly Lys Asn Ile Gln Asn Thr Phe Arg Ala 
                725                 730                 735     


Glu Ile Glu Glu Leu Ile Cys Gly Lys Phe Gly Ile Arg Leu Gly Ser 
            740                 745                 750         


Leu Phe His His Ser Leu Gln Phe Leu Thr Asn Cys Lys Ser Leu Ile 
        755                 760                 765             


Ser Ser Tyr Phe Met Leu Asn Asn Lys Lys Glu Glu Tyr Asp Gln Glu 
    770                 775                 780                 


Leu Phe Asp Ser Asp Phe Phe Arg Leu Met Lys Ser Ile Gly Asp Lys 
785                 790                 795                 800 


Arg Val Arg Lys Arg Lys Glu Lys Ser Ser Arg Ile Ser Ser Thr Val 
                805                 810                 815     


Leu Gln Ile Ala Arg Glu Asn Asn Val Lys Ser Leu Cys Val Glu Gly 
            820                 825                 830         


Tyr Leu Pro Thr Ser Thr Lys Lys Thr Lys Pro Lys Gln Asn Gln Lys 
        835                 840                 845             


Ser Ile Asp Trp Cys Ala Arg Ala Val Val Lys Lys Leu Asn Asp Gly 
    850                 855                 860                 


Cys Lys Val Leu Gly Ile Tyr Leu Gln Ala Ile Asp Pro Arg Asp Thr 
865                 870                 875                 880 


Ser His Leu Asp Pro Phe Val Tyr Tyr Gly Lys Lys Ser Thr Lys Val 
                885                 890                 895     


Gly Lys Glu Ala Arg His Thr Ile Val Glu Pro Ser Asn Ile Lys Glu 
            900                 905                 910         


Tyr Met Thr Asn Arg Phe Asp Asp Trp His Arg Gly Val Thr Lys Lys 
        915                 920                 925             


Ser Lys Lys Gly Asp Val Gln Thr Ser Thr Thr Val Leu Leu Tyr Gln 
    930                 935                 940                 


Glu Ala Leu Arg Gln Phe Ala Ser His Tyr Lys Leu Asp Phe Asp Ser 
945                 950                 955                 960 


Leu Pro Lys Met Lys Phe Tyr Glu Leu Ala Lys Ile Leu Gly Asp His 
                965                 970                 975     


Glu Lys Val Ile Ile Pro Cys Arg Gly Gly Arg Ala Tyr Leu Ser Thr 
            980                 985                 990         


Tyr Pro Val Thr Lys Asp Ser Ser  Lys Ile Thr Phe Asn  Gly Arg Glu 
        995                 1000                 1005             


Arg Trp  Tyr Asn Glu Ser Asp  Val Val Ala Ala Val  Asn Ile Val 
    1010                 1015                 1020             


Leu Arg  Gly Ile Ile Asp Glu  Asp Glu Gln Pro Asp  Gly Ala Lys 
    1025                 1030                 1035             


Lys Gln  Ala Thr Thr Arg Arg  Thr 
    1040                 1045     


<210>  3
<211>  1033
<212>  PRT
<213>  ˹

<220>
<223>  Cas12i.3İ

<400>  3

Met Met Ser Asp Asn Ile Ile Leu Pro Tyr Asn Ser Lys Leu Ala Pro 
1               5                   10                  15      


Asp Glu Arg Lys Gln Arg Leu Leu Asn Asp Thr Phe Asn Trp Phe Asp 
            20                  25                  30          


Met Cys Asn Glu Val Phe Phe Asp Phe Val Lys Asn Leu Tyr Gly Gly 
        35                  40                  45              


Val Lys His Glu His Leu Ile Leu Val Asn Phe Ala Glu Lys Pro Lys 
    50                  55                  60                  


Lys Val Ser Asn Ser Lys Lys Pro Lys Lys Lys Asp Gln Glu Val Asn 
65                  70                  75                  80  


Ile His Val Glu Pro Asn Gln Ala Glu Trp Val Asp Asn Ala Cys Ala 
                85                  90                  95      


Thr Phe Trp Phe Arg Leu Gln Ala Lys Ser Thr Val Gln Leu Asp Gln 
            100                 105                 110         


Ser Val Gln Thr Ala Glu Glu Arg Ile Arg Arg Phe Arg Asp Tyr Ala 
        115                 120                 125             


Gly His Glu Pro Ser Ser Phe Ala Lys Ser Tyr Leu Asn Gly Asn Tyr 
    130                 135                 140                 


Asp Pro Glu Lys Thr Glu Trp Val Asp Cys Arg Leu Leu Tyr Val Asn 
145                 150                 155                 160 


Phe Cys Arg Asn Leu Asn Val Asn Leu Asp Ala Asp Ile Arg Thr Met 
                165                 170                 175     


Val Glu His Asn Leu Leu Pro Val Leu Pro Gly Gln Asp Phe Lys Thr 
            180                 185                 190         


Asn Asn Val Phe Ser Asn Ile Phe Gly Val Gly Asn Lys Glu Asp Lys 
        195                 200                 205             


Gly Gln Lys Thr Asn Trp Leu Asn Thr Val Ser Glu Gly Leu Gln Ser 
    210                 215                 220                 


Lys Glu Ile Trp Asn Trp Asp Glu Tyr Arg Asp Leu Ile Ser Arg Ser 
225                 230                 235                 240 


Thr Gly Cys Ser Thr Ala Ala Glu Leu Arg Ser Glu Ser Ile Gly Arg 
                245                 250                 255     


Pro Ser Met Leu Ala Val Asp Phe Ala Ser Glu Lys Ser Gly Gln Ile 
            260                 265                 270         


Ser Gln Glu Trp Leu Ala Glu Arg Val Lys Ser Phe Arg Ala Ala Ala 
        275                 280                 285             


Ser Gln Lys Ser Lys Ile Tyr Asp Met Pro Asn Arg Leu Val Leu Lys 
    290                 295                 300                 


Glu Tyr Ile Ala Ser Lys Ile Gly Pro Phe Lys Leu Glu Arg Trp Ser 
305                 310                 315                 320 


Ala Ala Ala Val Ser Ala Tyr Lys Asp Val Arg Ser Lys Asn Ser Ile 
                325                 330                 335     


Asn Leu Leu Tyr Ser Lys Glu Arg Leu Trp Arg Cys Lys Glu Ile Ala 
            340                 345                 350         


Gln Ile Leu Val Asp Asn Thr Gln Val Ala Glu Ala Gln Gln Ile Leu 
        355                 360                 365             


Val Asn Tyr Ser Ser Gly Asp Thr Asn Ser Phe Thr Val Glu Asn Arg 
    370                 375                 380                 


His Met Gly Asp Leu Thr Val Leu Phe Lys Ile Trp Glu Lys Met Asp 
385                 390                 395                 400 


Met Asp Ser Gly Ile Glu Gln Tyr Ser Glu Ile Tyr Arg Asp Glu Tyr 
                405                 410                 415     


Ser Arg Asp Pro Ile Thr Glu Leu Leu Arg Tyr Leu Tyr Asn His Arg 
            420                 425                 430         


His Ile Ser Ala Lys Thr Phe Arg Ala Ala Ala Arg Leu Asn Ser Leu 
        435                 440                 445             


Leu Leu Lys Asn Asp Arg Lys Lys Ile His Pro Thr Ile Ser Gly Arg 
    450                 455                 460                 


Thr Ser Val Ser Phe Gly His Ser Thr Ile Lys Gly Cys Ile Thr Pro 
465                 470                 475                 480 


Pro Asp His Ile Val Lys Asn Arg Lys Glu Asn Ala Gly Ser Thr Gly 
                485                 490                 495     


Met Ile Trp Val Thr Met Gln Leu Ile Asp Asn Gly Arg Trp Ala Asp 
            500                 505                 510         


His His Ile Pro Phe His Asn Ser Arg Tyr Tyr Arg Asp Phe Tyr Ala 
        515                 520                 525             


Tyr Arg Ala Asp Leu Pro Thr Ile Ser Asp Pro Arg Arg Lys Ser Phe 
    530                 535                 540                 


Gly His Arg Ile Gly Asn Asn Ile Ser Asp Thr Arg Met Ile Asn His 
545                 550                 555                 560 


Asp Cys Lys Lys Ala Ser Lys Met Tyr Leu Arg Thr Ile Gln Asn Met 
                565                 570                 575     


Thr His Asn Val Ala Phe Asp Gln Gln Thr Gln Phe Ala Val Arg Arg 
            580                 585                 590         


Tyr Ala Asp Asn Asn Phe Thr Ile Thr Ile Gln Ala Arg Val Val Gly 
        595                 600                 605             


Arg Lys Tyr Lys Lys Glu Ile Ser Val Gly Asp Arg Val Met Gly Val 
    610                 615                 620                 


Asp Gln Asn Gln Thr Thr Ser Asn Thr Tyr Ser Val Trp Glu Val Val 
625                 630                 635                 640 


Ala Glu Gly Thr Glu Asn Ser Tyr Pro Tyr Lys Gly Asn Asn Tyr Arg 
                645                 650                 655     


Leu Val Glu Asp Gly Phe Ile Arg Ser Glu Cys Ser Gly Arg Asp Gln 
            660                 665                 670         


Leu Ser Tyr Asp Gly Leu Asp Phe Gln Asp Phe Ala Gln Trp Arg Arg 
        675                 680                 685             


Glu Arg Tyr Ala Phe Leu Ser Ser Val Gly Cys Ile Leu Asn Asp Glu 
    690                 695                 700                 


Ile Glu Pro Gln Ile Pro Val Ser Ala Glu Lys Ala Lys Lys Lys Lys 
705                 710                 715                 720 


Lys Phe Ser Lys Trp Arg Gly Cys Ser Leu Tyr Ser Trp Asn Leu Cys 
                725                 730                 735     


Tyr Ala Tyr Tyr Leu Lys Gly Leu Met His Glu Asn Leu Ala Asn Asn 
            740                 745                 750         


Pro Ala Gly Phe Arg Gln Glu Ile Leu Asn Phe Ile Gln Gly Ser Arg 
        755                 760                 765             


Gly Val Arg Leu Cys Ser Leu Asn His Thr Ser Phe Arg Leu Leu Ser 
    770                 775                 780                 


Lys Ala Lys Ser Leu Ile His Ser Phe Phe Gly Leu Asn Asn Ile Lys 
785                 790                 795                 800 


Asp Pro Glu Ser Gln Arg Asp Phe Asp Pro Glu Ile Tyr Asp Ile Met 
                805                 810                 815     


Val Asn Leu Thr Gln Arg Lys Thr Asn Lys Arg Lys Glu Lys Ala Asn 
            820                 825                 830         


Arg Ile Thr Ser Ser Ile Leu Gln Ile Ala Asn Arg Leu Asn Val Ser 
        835                 840                 845             


Arg Ile Val Ile Glu Asn Asp Leu Pro Asn Ala Ser Ser Lys Asn Lys 
    850                 855                 860                 


Ala Ser Ala Asn Gln Arg Ala Thr Asp Trp Cys Ala Arg Asn Val Ser 
865                 870                 875                 880 


Glu Lys Leu Glu Tyr Ala Cys Lys Met Leu Gly Ile Ser Leu Trp Gln 
                885                 890                 895     


Ile Asp Pro Arg Asp Thr Ser His Leu Asp Pro Phe Val Val Gly Lys 
            900                 905                 910         


Glu Ala Arg Phe Met Lys Ile Lys Val Ser Asp Ile Asn Glu Tyr Thr 
        915                 920                 925             


Ile Ser Asn Phe Lys Lys Trp His Ala Asn Ile Ala Thr Thr Ser Thr 
    930                 935                 940                 


Thr Ala Pro Leu Tyr His Asp Ala Leu Lys Ala Phe Ser Ser His Tyr 
945                 950                 955                 960 


Gly Ile Asp Trp Asp Asn Leu Pro Glu Met Lys Phe Trp Glu Leu Lys 
                965                 970                 975     


Asn Ala Leu Lys Asp His Lys Glu Val Phe Ile Pro Asn Arg Gly Gly 
            980                 985                 990         


Arg Cys Tyr Leu Ser Thr Leu Pro  Val Thr Ser Thr Ser  Glu Lys Ile 
        995                 1000                 1005             


Val Phe  Asn Gly Arg Glu Arg  Trp Leu Asn Ala Ser  Asp Ile Val 
    1010                 1015                 1020             


Ala Gly  Val Asn Ile Val Leu  Arg Ser Val 
    1025                 1030             


<210>  4
<211>  3282
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.1ı

<400>  4
atgtctaaca aagaaaaaaa tgcaagcgaa actcgcaaag cctacacaac aaaaatgatt       60

ccaagaagcc atgatcgcat gaaattgctt gggaatttca tggattattt gatggatgga      120

acgccaatat ttttcgaact ttggaatcag tttggcggcg ggattgaccg cgatatcatt      180

tctggcactg caaataaaga caagatatca gatgatttac ttttggcggt caattggttc      240

aaggtaatgc caattaattc taagcctcaa ggtgtatcgc catcaaatct tgccaacctc      300

tttcaacaat actctggatc agaaccagac attcaagctc aagagtattt tgcttcaaat      360

tttgacaccg aaaagcatca atggaaggac atgcgtgttg aatacgaacg actattagct      420

gaattgcagc tatcgagaag tgatatgcat catgacttga agctcatgta caaagaaaaa      480

tgcattggcc taagtctttc tacggctcac tacatcactt ctgtgatgtt tgggacagga      540

gctaaaaaca atcgccaaac caagcatcaa ttctatagca aggttatcca actacttgag      600

gaatcaactc aaatcaattc tgttgaacag ttggcatcta ttattttgaa agcaggagat      660

tgcgatagtt atcgaaagct tcgtattcga tgttctcgta agggagcaac acccagcatt      720

cttaagatcg ttcaagacta tgaactggga accaatcacg atgatgaagt gaatgtgcca      780

agtttgattg caaatttgaa agaaaaattg ggcagatttg aatatgaatg cgaatggaag      840

tgcatggaaa aaatcaaagc atttttagct agcaaagttg ggccttatta cctaggctct      900

tacagtgcga tgcttgaaaa tgcattgtcg cccatcaagg gaatgactac aaaaaattgc      960

aaatttgtgt taaagcaaat tgatgccaaa aacgacatca agtatgaaaa tgagccattt     1020

ggcaaaattg ttgaagggtt ttttgactct ccatattttg aaagcgacac caatgtgaaa     1080

tgggttttgc acccacatca tattggagaa agcaatatca aaacactctg ggaagacttg     1140

aatgcaattc attctaagta cgaagaagat attgcttctt tgagcgaaga caaaaaagag     1200

aaacgcatta aggtttatca aggagatgtt tgccaaacaa tcaatacgta ttgtgaagaa     1260

gtaggaaagg aagctaagac tcctttagtt cagcttttgc gttatcttta ctctagaaaa     1320

gatgatattg ctgttgataa gataattgat ggcattacct tccttagcaa gaaacacaag     1380

gttgaaaaac aaaaaatcaa tcctgtaatt caaaaatatc ccagtttcaa ctttgggaat     1440

aattctaagt tgttgggaaa gattatcagc cccaaagaca agttaaagca taatctcaaa     1500

tgcaacagga atcaggttga taattacatt tggattgaga ttaaagtact aaacaccaaa     1560

acgatgcgat gggaaaagca tcactatgct ttatcatcta cgcggttttt ggaagaggtc     1620

tattatccag ccacatccga aaatccgcca gacgctttgg cagcacgttt ccgaactaaa     1680

actaatgggt atgaaggcaa gcctgcgttg tctgctgagc aaattgaaca aattagatca     1740

gccccagtcg gtttgagaaa agtgaaaaaa cgtcaaatgc gactcgaagc tgcaagacag     1800

caaaatctct tgcctcgata cacttggggc aaagatttca acataaacat ttgtaagcgt     1860

ggcaacaatt ttgaagtcac tcttgcgacg aaggtgaaaa agaaaaaaga aaagaattat     1920

aaggttgttt tagggtacga tgctaatatc gttcgcaaaa acacttacgc agccatagaa     1980

gctcacgcta atggcgatgg tgtgattgac tacaatgact tgcccgtgaa gcctattgaa     2040

agtggatttg taaccgttga aagtcaagtg cgagacaaat cttacgatca actctcttac     2100

aatggcgtaa agctcttgta ttgcaagcct catgttgagt ctcgacgttc atttttggag     2160

aaataccgaa atggcaccat gaaggacaac agaggaaaca acattcaaat tgactttatg     2220

aaagactttg aagctattgc ggatgatgaa acttctttgt attacttcaa tatgaagtac     2280

tgcaagctgc ttcaatcgtc cattcgcaat cattcttcac aagcaaaaga atatcgtgaa     2340

gagatttttg aattgttaag agacggaaaa ctatcggttt tgaagttatc atctttgagc     2400

aatctttctt ttgtgatgtt caaagttgcc aaatctctga tcggtactta ctttggccac     2460

ttgcttaaga agccgaagaa ttctaagtca gatgttaagg caccgcctat aactgatgaa     2520

gataagcaaa aagctgatcc tgagatgttt gctttgaggt tggctttgga ggagaagcga     2580

ctaaacaaag tcaagtctaa gaaagaagta attgcgaaca agattgttgc taaggcactt     2640

gagcttcgcg acaagtacgg gcctgtgttg attaagggag aaaacatctc tgacacgacc     2700

aagaaaggca agaagtcaag caccaattct tttttgatgg actggctagc acgcggtgtg     2760

gctaataaag tcaaagaaat ggtaatgatg catcaaggac ttgaatttgt agaagtaaat     2820

cctaatttca catctcacca agatcctttt gttcacaaga accctgaaaa tacgtttaga     2880

gctaggtaca gtcggtgcac tccaagtgaa cttactgaga aaaatcgcaa ggaaattttg     2940

agctttttga gcgataagcc ttctaaacga ccgacaaatg cctattacaa tgaaggtgcg     3000

atggcctttc ttgcaactta tggcttgaag aagaatgatg tgctaggagt tagtcttgag     3060

aaattcaagc aaataatggc caacattcta catcagcgtt ccgaagatca attattgttt     3120

ccttctagag gtggcatgtt ttatcttgca acttacaagc ttgatgctga cgctacctct     3180

gtaaattgga atggcaaaca gttttgggtt tgtaacgcag atttagtagc ggcatacaat     3240

gtcggtttgg tcgatattca aaaagacttc aagaaaaagt aa                        3282


<210>  5
<211>  3141
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.2ı

<400>  5
atggtcagcg aaagtacgat ccgtccttat accagcaaat tggcaccaaa tgattcaaag       60

ctgaaaatgc ttaacgatac attcaattgg ctagaccatg catacaaggt attctttgat      120

gtatcagtag cactttttgg tgccattgaa catgaaactg ctcaagaact gataggtgaa      180

aaaagtaaat tcgacgcaga tctactctgt gctatcatgt ggtttcgcct agaagaaaaa      240

tcagataacc ccggacctct ccagacagta gaacaaagga tgagactatt ccagaaatac      300

tctggacacg aaccatcttc tttcacacaa gaatacatca aaggaaacat agattcagaa      360

aaataccaat gggtagattg tcgtctaaaa tttatagact tagctagaaa tattaacaca      420

actcaagaat cactcaaaat tgatgcatac actctcttca tgaataaatt aattcctgtg      480

agcaaagatg atgaattcaa cgcttatggc ttgatttcac aactttttgg aacaggaaag      540

aaagaagacc gatcaatcaa agcatcaatg cttgaagaaa tctcaaatat tctcgcagac      600

aaaaatccaa acacttggga agaatatcag gatttaatta aaaaaacttt caatgttgat      660

aattacaaag aacttaaaga aaaattaagc gcaggaagca gtggtcgtga tggatctcta      720

gtcattgacc tcaaagaaga aaaaacagga ttacttcaac ctaattttat caaaaatcgt      780

attgttaaat tcagagaaga tgctgacaag aagagaactg tatttctatt gcccaataga      840

atgaagttga gagaatttat tgcttcgcaa attggaccat ttgaacaaaa tagttggtcg      900

gctgttctaa acagatctat ggccgcaatc caatcaaaaa atagcagcaa cattctatac      960

actaatgaaa aagaagaacg caataatgaa attcaagaat tgttgaaaaa agacatcttg     1020

tcagcagcaa gtatattagg cgattttcgt cgtggagaat ttaacagatc agtggtttca     1080

aaaaatcact tgggagcaag actcaatgag ctttttgaaa tatggcaaga attaacaatg     1140

gatgatggaa tcaaaaaata tgttgatctt tgtaaagata agttttccag aagacctgta     1200

aaggcattgc ttcaatacat ttatccatat ttcgataaaa ttaatgcaaa gcaatttctt     1260

gatgcagcta gttacaacac acttgttgaa accaataatc gcaagaagat tcacccaact     1320

gtcacaggac caacagtttg taattgggga ccgaagtcta caattaatgg atcaataaca     1380

ccaccaaatc aaatggtcaa aggaagacca gcaggatctc atggaatgat ttgggtcaca     1440

atgacagtca tagataatgg acgttggatc aagcatcacc ttccattcca taactcacgt     1500

tattacgaag aacactattg ctacagagaa ggtttgccta caaaaaataa acctcgtact     1560

aaacaacttg gtactcaagt aggatcaaca atttccgctc caagtcttgc tattcttaaa     1620

tctcaagaag aacaagatag aaggaatgat cgtaaaaata gattcaaagc ccacaaatct     1680

atcatcagat cacaagagaa cattgaatac aatgttgcct ttgacaagtc aactaatttt     1740

gacgtaacac gaaaaaatgg tgagtttttc atcactatct cttctagagt tgctactcca     1800

aaatatagtt ataaattaaa tattggcgat atgatcatgg gactggacaa caaccaaaca     1860

gccccatgca catattcaat ttggcgtgta gtggagaaag atacagaagg tagtttcttt     1920

cataataaaa tttggctcca attggtaact gacggtaaag taacaagtat tgttgacaat     1980

aaccgtcaag tcgatcagct ttcttatgct ggtattgaat actccaattt tgctgaatgg     2040

agaaaagatc gtcgccaatt ccttcgatca attaacgaag attacgttaa aaaatcagat     2100

aattggcgta atatgaatct ttatcaatgg aatgctgaat attctcgttt gcttcttgat     2160

gtcatgaagg aaaataaggg caaaaatatt caaaatacat tccgtgcaga aatagaagaa     2220

ttaatttgtg gtaagttcgg tataagattg ggaagtcttt ttcatcattc ccttcaattt     2280

cttactaatt gtaagagtct tatatcatct tattttatgc ttaacaataa aaaagaagag     2340

tatgatcaag agttgtttga cagtgatttc tttaggttga tgaaaagtat tggggacaaa     2400

cgtgttagga aacgcaaaga gaaatcttca aggatttcat ctacagtatt gcaaattgcg     2460

agggaaaata atgtcaagtc tttgtgtgtc gagggttatt tgcctacatc cacaaagaag     2520

actaagccaa aacaaaatca aaaatcaata gattggtgtg ctcgtgctgt tgttaaaaaa     2580

ttgaatgatg gttgtaaggt tttgggtatt tatctacagg ctattgatcc aagagatacg     2640

agtcatttag atccatttgt ctattatgga aagaaatcta ctaaagttgg aaaagaagct     2700

cgacacacaa ttgttgagcc atccaatata aaggaataca tgacaaacag attcgatgac     2760

tggcatcgag gtgttaccaa aaagtcaaaa aagggtgatg ttcaaacaag cactactgtt     2820

cttctttatc aagaagcttt aaggcaattt gctagccatt acaaacttga ttttgactct     2880

ttgccaaaaa tgaaattcta tgaattagct aaaatattgg gagatcatga aaaagtgatt     2940

atcccttgtc gtggaggaag agcttatctt tctacttatc cagtaacaaa agattcctcg     3000

aaaataactt tcaatggtag agaaagatgg tataatgaat cagatgtggt agctgctgtt     3060

aacatcgtgc tgagaggcat aatagatgaa gacgagcagc ctgatggtgc caaaaaacag     3120

gcaaccactc gcagaacgta a                                               3141


<210>  6
<211>  3102
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.3ı

<400>  6
atgatgtctg ataatattat tctgccttac aactctaaac ttgcccccga tgagcgtaag       60

caaaggcttc tgaacgacac attcaattgg tttgatatgt gtaacgaagt tttttttgac      120

ttcgtgaaga atctgtatgg cggagtgaag catgaacatt tgattcttgt gaattttgcc      180

gagaaaccca agaaggttag caatagtaaa aagcctaaga aaaaagatca ggaagtcaac      240

attcatgttg agcccaatca agccgaatgg gttgacaatg cttgtgccac attctggttt      300

cgattgcagg caaaatcaac agtacaatta gaccaatcag tccagacagc agaagagcgt      360

attcgacgat ttcgggatta tgctggtcac gagccatcat catttgccaa atcttatcta      420

aatggtaatt atgatccaga gaagactgaa tgggttgatt gcaggcttct ttatgtcaat      480

ttttgccgta atctgaatgt caatcttgat gcggacattc gcacaatggt cgaacacaat      540

cttcttcctg ttctccccgg tcaggatttc aaaaccaaca atgtattctc caacattttt      600

ggagtaggca ataaggaaga caagggtcaa aaaacgaact ggcttaacac ggtctcagaa      660

ggccttcagt ccaaggaaat ttggaattgg gatgagtatc gtgatttgat atcaagatct      720

actggatgct ctacggcagc agaactgagg tctgagtcga ttggcaggcc aagcatgctt      780

gcagttgatt ttgcatctga gaaatcaggc caaatatcac aggaatggct tgcagaaagg      840

gttaagtctt ttagggcagc agcttctcaa aaaagcaaaa tctatgacat gcctaatcgt      900

cttgttttga aggaatacat tgcttcaaaa atcgggcctt tcaaacttga aaggtggtct      960

gctgctgccg tttctgctta taaggatgtc cgtagcaaga atagtatcaa tttgctttat     1020

tccaaggaaa gattgtggcg atgcaaggaa attgctcaga ttttggttga taatacgcag     1080

gttgctgaag cccaacagat tcttgttaat tattcttctg gtgataccaa ttcattcaca     1140

gttgaaaatc gtcacatggg cgatttgact gttcttttca agatttggga aaagatggat     1200

atggattctg gcatagaaca gtattccgaa atttatcgtg atgaatatag tcgtgatcca     1260

attacagagt tgctacgcta tctctacaat cataggcata tttcggcaaa aacttttagg     1320

gctgctgcaa ggttgaattc tcttttgctg aagaatgatc gcaagaaaat tcacccgact     1380

atcagtggta ggactagcgt ttctttcggc cattcaacaa tcaagggatg cattactcct     1440

cccgatcata ttgtcaaaaa tcgaaaagag aatgctggaa gcactggcat gatctgggtt     1500

acaatgcagc ttatcgataa tggtcgatgg gcggatcatc atattccttt ccacaattct     1560

cgctactatc gtgattttta tgcctatcgt gccgatctac cgactatttc tgatcctcgt     1620

cgtaaatctt ttggacatag gatcggcaat aatattagcg atacaaggat gattaatcat     1680

gattgcaaaa aagcatcaaa aatgtatctt cgcacaatcc agaatatgac gcacaatgtg     1740

gcattcgacc agcagactca gttcgctgtt cgtcgttatg ctgataataa tttcacgatt     1800

acgatccaag ctagggttgt agggaggaaa tataaaaagg aaatatcagt tggtgatcgt     1860

gtgatgggtg ttgaccagaa tcagaccaca agtaacacat attctgtttg ggaagtagtt     1920

gctgaaggga ctgaaaattc ttacccatat aagggcaata attatcgtct cgttgaggat     1980

ggatttattc gcagcgaatg cagtggtcga gatcagcttt cctatgatgg tcttgatttt     2040

caggactttg ctcaatggcg cagggaaaga tacgcatttt tatcttctgt tggatgtatt     2100

ctcaatgacg aaatcgagcc tcaaatccct gttagtgccg aaaaggcaaa aaagaagaag     2160

aagttttcta agtggcgtgg ttgttctctt tatagttgga acctctgtta tgcatattat     2220

cttaagggtt tgatgcatga gaacttggca aataatcctg ctggattccg acaggaaatt     2280

ctgaatttta ttcagggttc taggggcgtg aggctttgtt cccttaatca cactagcttc     2340

cgacttctct ctaaagccaa gtctctaatt cattcatttt ttggtctaaa caatatcaaa     2400

gatcctgaat ctcaaaggga ttttgatcca gaaatttatg acataatggt caatctgaca     2460

caaaggaaga ccaacaagag gaaggaaaaa gctaatcgca tcacttcttc aattttgcaa     2520

attgccaata ggttgaatgt cagtcgcatt gtgattgaga acgatttgcc gaatgcaagt     2580

tccaaaaaca aggcatcagc caatcaaagg gcaactgatt ggtgtgctag aaatgtatct     2640

gagaaattag aatatgcctg caagatgctt ggtatcagct tatggcagat tgatccaagg     2700

gacacatcgc accttgatcc atttgttgtg ggcaaggagg ctagatttat gaaaataaag     2760

gtttctgata ttaacgaata cactatcagt aatttcaaaa agtggcatgc aaacattgct     2820

acaacaagta ctacagcacc tctttatcat gatgctttga aggcattctc ttctcattat     2880

ggaattgatt gggacaattt gccagaaatg aagttttggg aattgaagaa tgccttgaaa     2940

gaccataaag aggtgtttat cccaaatcgt ggtggtcgct gctatttatc gacattgccg     3000

gtgacttcta catctgaaaa gattgttttc aatggaagag agagatggtt gaacgcaagt     3060

gatattgtgg caggagttaa cattgtgcta agatcagtat ga                        3102


<210>  7
<211>  64
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.1/ԭͬظ

<400>  7
cguuggaaug acuaauuuuu gugcccaccg uuggcacggu auaacaacuu cgacgagcuc       60

uaca                                                                    64


<210>  8
<211>  62
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.2/ԭͬظ

<400>  8
ccacaauacc ugagaaaucc guccuacguu gacgggguau aacaacuucg acgagcucua       60

ca                                                                      62


<210>  9
<211>  63
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.3/ԭͬظ

<400>  9
cucgcaaugc cuuagaaauc cguccuuggu ugacggggua uaacaacuuc gacgagcucu       60

aca                                                                     63


<210>  10
<211>  64
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.1/ԭͬظеı

<400>  10
cgttggaatg actaattttt gtgcccaccg ttggcacggt ataacaactt cgacgagctc       60

taca                                                                    64


<210>  11
<211>  62
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.2/ԭͬظеı

<400>  11
ccacaatacc tgagaaatcc gtcctacgtt gacggggtat aacaacttcg acgagctcta       60

ca                                                                      62


<210>  12
<211>  63
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.3/ԭͬظеı

<400>  12
ctcgcaatgc cttagaaatc cgtccttggt tgacggggta taacaacttc gacgagctct       60

aca                                                                     63


<210>  13
<211>  23
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.1/ͬظ

<400>  13
auuuuugugc ccaccguugg cac                                               23


<210>  14
<211>  23
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.2/ͬظ

<400>  14
agaaauccgu ccuacguuga cgg                                               23


<210>  15
<211>  23
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.3/ͬظ

<400>  15
agaaauccgu ccuugguuga cgg                                               23


<210>  16
<211>  23
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.1/ͬظеı

<400>  16
atttttgtgc ccaccgttgg cac                                               23


<210>  17
<211>  23
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.2/ͬظеı

<400>  17
agaaatccgt cctacgttga cgg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.3/ͬظеı

<400>  18
agaaatccgt ccttggttga cgg                                               23


<210>  19
<211>  11
<212>  PRT
<213>  ˹

<220>
<223>  NLS

<400>  19

Ser Arg Ala Asp Pro Lys Lys Lys Arg Lys Val 
1               5                   10      


<210>  20
<211>  1104
<212>  PRT
<213>  ˹

<220>
<223>  Cas12i.1-NLSںϵ׵İ

<400>  20

Met Ser Asn Lys Glu Lys Asn Ala Ser Glu Thr Arg Lys Ala Tyr Thr 
1               5                   10                  15      


Thr Lys Met Ile Pro Arg Ser His Asp Arg Met Lys Leu Leu Gly Asn 
            20                  25                  30          


Phe Met Asp Tyr Leu Met Asp Gly Thr Pro Ile Phe Phe Glu Leu Trp 
        35                  40                  45              


Asn Gln Phe Gly Gly Gly Ile Asp Arg Asp Ile Ile Ser Gly Thr Ala 
    50                  55                  60                  


Asn Lys Asp Lys Ile Ser Asp Asp Leu Leu Leu Ala Val Asn Trp Phe 
65                  70                  75                  80  


Lys Val Met Pro Ile Asn Ser Lys Pro Gln Gly Val Ser Pro Ser Asn 
                85                  90                  95      


Leu Ala Asn Leu Phe Gln Gln Tyr Ser Gly Ser Glu Pro Asp Ile Gln 
            100                 105                 110         


Ala Gln Glu Tyr Phe Ala Ser Asn Phe Asp Thr Glu Lys His Gln Trp 
        115                 120                 125             


Lys Asp Met Arg Val Glu Tyr Glu Arg Leu Leu Ala Glu Leu Gln Leu 
    130                 135                 140                 


Ser Arg Ser Asp Met His His Asp Leu Lys Leu Met Tyr Lys Glu Lys 
145                 150                 155                 160 


Cys Ile Gly Leu Ser Leu Ser Thr Ala His Tyr Ile Thr Ser Val Met 
                165                 170                 175     


Phe Gly Thr Gly Ala Lys Asn Asn Arg Gln Thr Lys His Gln Phe Tyr 
            180                 185                 190         


Ser Lys Val Ile Gln Leu Leu Glu Glu Ser Thr Gln Ile Asn Ser Val 
        195                 200                 205             


Glu Gln Leu Ala Ser Ile Ile Leu Lys Ala Gly Asp Cys Asp Ser Tyr 
    210                 215                 220                 


Arg Lys Leu Arg Ile Arg Cys Ser Arg Lys Gly Ala Thr Pro Ser Ile 
225                 230                 235                 240 


Leu Lys Ile Val Gln Asp Tyr Glu Leu Gly Thr Asn His Asp Asp Glu 
                245                 250                 255     


Val Asn Val Pro Ser Leu Ile Ala Asn Leu Lys Glu Lys Leu Gly Arg 
            260                 265                 270         


Phe Glu Tyr Glu Cys Glu Trp Lys Cys Met Glu Lys Ile Lys Ala Phe 
        275                 280                 285             


Leu Ala Ser Lys Val Gly Pro Tyr Tyr Leu Gly Ser Tyr Ser Ala Met 
    290                 295                 300                 


Leu Glu Asn Ala Leu Ser Pro Ile Lys Gly Met Thr Thr Lys Asn Cys 
305                 310                 315                 320 


Lys Phe Val Leu Lys Gln Ile Asp Ala Lys Asn Asp Ile Lys Tyr Glu 
                325                 330                 335     


Asn Glu Pro Phe Gly Lys Ile Val Glu Gly Phe Phe Asp Ser Pro Tyr 
            340                 345                 350         


Phe Glu Ser Asp Thr Asn Val Lys Trp Val Leu His Pro His His Ile 
        355                 360                 365             


Gly Glu Ser Asn Ile Lys Thr Leu Trp Glu Asp Leu Asn Ala Ile His 
    370                 375                 380                 


Ser Lys Tyr Glu Glu Asp Ile Ala Ser Leu Ser Glu Asp Lys Lys Glu 
385                 390                 395                 400 


Lys Arg Ile Lys Val Tyr Gln Gly Asp Val Cys Gln Thr Ile Asn Thr 
                405                 410                 415     


Tyr Cys Glu Glu Val Gly Lys Glu Ala Lys Thr Pro Leu Val Gln Leu 
            420                 425                 430         


Leu Arg Tyr Leu Tyr Ser Arg Lys Asp Asp Ile Ala Val Asp Lys Ile 
        435                 440                 445             


Ile Asp Gly Ile Thr Phe Leu Ser Lys Lys His Lys Val Glu Lys Gln 
    450                 455                 460                 


Lys Ile Asn Pro Val Ile Gln Lys Tyr Pro Ser Phe Asn Phe Gly Asn 
465                 470                 475                 480 


Asn Ser Lys Leu Leu Gly Lys Ile Ile Ser Pro Lys Asp Lys Leu Lys 
                485                 490                 495     


His Asn Leu Lys Cys Asn Arg Asn Gln Val Asp Asn Tyr Ile Trp Ile 
            500                 505                 510         


Glu Ile Lys Val Leu Asn Thr Lys Thr Met Arg Trp Glu Lys His His 
        515                 520                 525             


Tyr Ala Leu Ser Ser Thr Arg Phe Leu Glu Glu Val Tyr Tyr Pro Ala 
    530                 535                 540                 


Thr Ser Glu Asn Pro Pro Asp Ala Leu Ala Ala Arg Phe Arg Thr Lys 
545                 550                 555                 560 


Thr Asn Gly Tyr Glu Gly Lys Pro Ala Leu Ser Ala Glu Gln Ile Glu 
                565                 570                 575     


Gln Ile Arg Ser Ala Pro Val Gly Leu Arg Lys Val Lys Lys Arg Gln 
            580                 585                 590         


Met Arg Leu Glu Ala Ala Arg Gln Gln Asn Leu Leu Pro Arg Tyr Thr 
        595                 600                 605             


Trp Gly Lys Asp Phe Asn Ile Asn Ile Cys Lys Arg Gly Asn Asn Phe 
    610                 615                 620                 


Glu Val Thr Leu Ala Thr Lys Val Lys Lys Lys Lys Glu Lys Asn Tyr 
625                 630                 635                 640 


Lys Val Val Leu Gly Tyr Asp Ala Asn Ile Val Arg Lys Asn Thr Tyr 
                645                 650                 655     


Ala Ala Ile Glu Ala His Ala Asn Gly Asp Gly Val Ile Asp Tyr Asn 
            660                 665                 670         


Asp Leu Pro Val Lys Pro Ile Glu Ser Gly Phe Val Thr Val Glu Ser 
        675                 680                 685             


Gln Val Arg Asp Lys Ser Tyr Asp Gln Leu Ser Tyr Asn Gly Val Lys 
    690                 695                 700                 


Leu Leu Tyr Cys Lys Pro His Val Glu Ser Arg Arg Ser Phe Leu Glu 
705                 710                 715                 720 


Lys Tyr Arg Asn Gly Thr Met Lys Asp Asn Arg Gly Asn Asn Ile Gln 
                725                 730                 735     


Ile Asp Phe Met Lys Asp Phe Glu Ala Ile Ala Asp Asp Glu Thr Ser 
            740                 745                 750         


Leu Tyr Tyr Phe Asn Met Lys Tyr Cys Lys Leu Leu Gln Ser Ser Ile 
        755                 760                 765             


Arg Asn His Ser Ser Gln Ala Lys Glu Tyr Arg Glu Glu Ile Phe Glu 
    770                 775                 780                 


Leu Leu Arg Asp Gly Lys Leu Ser Val Leu Lys Leu Ser Ser Leu Ser 
785                 790                 795                 800 


Asn Leu Ser Phe Val Met Phe Lys Val Ala Lys Ser Leu Ile Gly Thr 
                805                 810                 815     


Tyr Phe Gly His Leu Leu Lys Lys Pro Lys Asn Ser Lys Ser Asp Val 
            820                 825                 830         


Lys Ala Pro Pro Ile Thr Asp Glu Asp Lys Gln Lys Ala Asp Pro Glu 
        835                 840                 845             


Met Phe Ala Leu Arg Leu Ala Leu Glu Glu Lys Arg Leu Asn Lys Val 
    850                 855                 860                 


Lys Ser Lys Lys Glu Val Ile Ala Asn Lys Ile Val Ala Lys Ala Leu 
865                 870                 875                 880 


Glu Leu Arg Asp Lys Tyr Gly Pro Val Leu Ile Lys Gly Glu Asn Ile 
                885                 890                 895     


Ser Asp Thr Thr Lys Lys Gly Lys Lys Ser Ser Thr Asn Ser Phe Leu 
            900                 905                 910         


Met Asp Trp Leu Ala Arg Gly Val Ala Asn Lys Val Lys Glu Met Val 
        915                 920                 925             


Met Met His Gln Gly Leu Glu Phe Val Glu Val Asn Pro Asn Phe Thr 
    930                 935                 940                 


Ser His Gln Asp Pro Phe Val His Lys Asn Pro Glu Asn Thr Phe Arg 
945                 950                 955                 960 


Ala Arg Tyr Ser Arg Cys Thr Pro Ser Glu Leu Thr Glu Lys Asn Arg 
                965                 970                 975     


Lys Glu Ile Leu Ser Phe Leu Ser Asp Lys Pro Ser Lys Arg Pro Thr 
            980                 985                 990         


Asn Ala Tyr Tyr Asn Glu Gly Ala  Met Ala Phe Leu Ala  Thr Tyr Gly 
        995                 1000                 1005             


Leu Lys  Lys Asn Asp Val Leu  Gly Val Ser Leu Glu  Lys Phe Lys 
    1010                 1015                 1020             


Gln Ile  Met Ala Asn Ile Leu  His Gln Arg Ser Glu  Asp Gln Leu 
    1025                 1030                 1035             


Leu Phe  Pro Ser Arg Gly Gly  Met Phe Tyr Leu Ala  Thr Tyr Lys 
    1040                 1045                 1050             


Leu Asp  Ala Asp Ala Thr Ser  Val Asn Trp Asn Gly  Lys Gln Phe 
    1055                 1060                 1065             


Trp Val  Cys Asn Ala Asp Leu  Val Ala Ala Tyr Asn  Val Gly Leu 
    1070                 1075                 1080             


Val Asp  Ile Gln Lys Asp Phe  Lys Lys Lys Ser Arg  Ala Asp Pro 
    1085                 1090                 1095             


Lys Lys  Lys Arg Lys Val 
    1100                 


<210>  21
<211>  1057
<212>  PRT
<213>  ˹

<220>
<223>  Cas12i.2-NLSںϵ׵İ

<400>  21

Met Val Ser Glu Ser Thr Ile Arg Pro Tyr Thr Ser Lys Leu Ala Pro 
1               5                   10                  15      


Asn Asp Ser Lys Leu Lys Met Leu Asn Asp Thr Phe Asn Trp Leu Asp 
            20                  25                  30          


His Ala Tyr Lys Val Phe Phe Asp Val Ser Val Ala Leu Phe Gly Ala 
        35                  40                  45              


Ile Glu His Glu Thr Ala Gln Glu Leu Ile Gly Glu Lys Ser Lys Phe 
    50                  55                  60                  


Asp Ala Asp Leu Leu Cys Ala Ile Met Trp Phe Arg Leu Glu Glu Lys 
65                  70                  75                  80  


Ser Asp Asn Pro Gly Pro Leu Gln Thr Val Glu Gln Arg Met Arg Leu 
                85                  90                  95      


Phe Gln Lys Tyr Ser Gly His Glu Pro Ser Ser Phe Thr Gln Glu Tyr 
            100                 105                 110         


Ile Lys Gly Asn Ile Asp Ser Glu Lys Tyr Gln Trp Val Asp Cys Arg 
        115                 120                 125             


Leu Lys Phe Ile Asp Leu Ala Arg Asn Ile Asn Thr Thr Gln Glu Ser 
    130                 135                 140                 


Leu Lys Ile Asp Ala Tyr Thr Leu Phe Met Asn Lys Leu Ile Pro Val 
145                 150                 155                 160 


Ser Lys Asp Asp Glu Phe Asn Ala Tyr Gly Leu Ile Ser Gln Leu Phe 
                165                 170                 175     


Gly Thr Gly Lys Lys Glu Asp Arg Ser Ile Lys Ala Ser Met Leu Glu 
            180                 185                 190         


Glu Ile Ser Asn Ile Leu Ala Asp Lys Asn Pro Asn Thr Trp Glu Glu 
        195                 200                 205             


Tyr Gln Asp Leu Ile Lys Lys Thr Phe Asn Val Asp Asn Tyr Lys Glu 
    210                 215                 220                 


Leu Lys Glu Lys Leu Ser Ala Gly Ser Ser Gly Arg Asp Gly Ser Leu 
225                 230                 235                 240 


Val Ile Asp Leu Lys Glu Glu Lys Thr Gly Leu Leu Gln Pro Asn Phe 
                245                 250                 255     


Ile Lys Asn Arg Ile Val Lys Phe Arg Glu Asp Ala Asp Lys Lys Arg 
            260                 265                 270         


Thr Val Phe Leu Leu Pro Asn Arg Met Lys Leu Arg Glu Phe Ile Ala 
        275                 280                 285             


Ser Gln Ile Gly Pro Phe Glu Gln Asn Ser Trp Ser Ala Val Leu Asn 
    290                 295                 300                 


Arg Ser Met Ala Ala Ile Gln Ser Lys Asn Ser Ser Asn Ile Leu Tyr 
305                 310                 315                 320 


Thr Asn Glu Lys Glu Glu Arg Asn Asn Glu Ile Gln Glu Leu Leu Lys 
                325                 330                 335     


Lys Asp Ile Leu Ser Ala Ala Ser Ile Leu Gly Asp Phe Arg Arg Gly 
            340                 345                 350         


Glu Phe Asn Arg Ser Val Val Ser Lys Asn His Leu Gly Ala Arg Leu 
        355                 360                 365             


Asn Glu Leu Phe Glu Ile Trp Gln Glu Leu Thr Met Asp Asp Gly Ile 
    370                 375                 380                 


Lys Lys Tyr Val Asp Leu Cys Lys Asp Lys Phe Ser Arg Arg Pro Val 
385                 390                 395                 400 


Lys Ala Leu Leu Gln Tyr Ile Tyr Pro Tyr Phe Asp Lys Ile Asn Ala 
                405                 410                 415     


Lys Gln Phe Leu Asp Ala Ala Ser Tyr Asn Thr Leu Val Glu Thr Asn 
            420                 425                 430         


Asn Arg Lys Lys Ile His Pro Thr Val Thr Gly Pro Thr Val Cys Asn 
        435                 440                 445             


Trp Gly Pro Lys Ser Thr Ile Asn Gly Ser Ile Thr Pro Pro Asn Gln 
    450                 455                 460                 


Met Val Lys Gly Arg Pro Ala Gly Ser His Gly Met Ile Trp Val Thr 
465                 470                 475                 480 


Met Thr Val Ile Asp Asn Gly Arg Trp Ile Lys His His Leu Pro Phe 
                485                 490                 495     


His Asn Ser Arg Tyr Tyr Glu Glu His Tyr Cys Tyr Arg Glu Gly Leu 
            500                 505                 510         


Pro Thr Lys Asn Lys Pro Arg Thr Lys Gln Leu Gly Thr Gln Val Gly 
        515                 520                 525             


Ser Thr Ile Ser Ala Pro Ser Leu Ala Ile Leu Lys Ser Gln Glu Glu 
    530                 535                 540                 


Gln Asp Arg Arg Asn Asp Arg Lys Asn Arg Phe Lys Ala His Lys Ser 
545                 550                 555                 560 


Ile Ile Arg Ser Gln Glu Asn Ile Glu Tyr Asn Val Ala Phe Asp Lys 
                565                 570                 575     


Ser Thr Asn Phe Asp Val Thr Arg Lys Asn Gly Glu Phe Phe Ile Thr 
            580                 585                 590         


Ile Ser Ser Arg Val Ala Thr Pro Lys Tyr Ser Tyr Lys Leu Asn Ile 
        595                 600                 605             


Gly Asp Met Ile Met Gly Leu Asp Asn Asn Gln Thr Ala Pro Cys Thr 
    610                 615                 620                 


Tyr Ser Ile Trp Arg Val Val Glu Lys Asp Thr Glu Gly Ser Phe Phe 
625                 630                 635                 640 


His Asn Lys Ile Trp Leu Gln Leu Val Thr Asp Gly Lys Val Thr Ser 
                645                 650                 655     


Ile Val Asp Asn Asn Arg Gln Val Asp Gln Leu Ser Tyr Ala Gly Ile 
            660                 665                 670         


Glu Tyr Ser Asn Phe Ala Glu Trp Arg Lys Asp Arg Arg Gln Phe Leu 
        675                 680                 685             


Arg Ser Ile Asn Glu Asp Tyr Val Lys Lys Ser Asp Asn Trp Arg Asn 
    690                 695                 700                 


Met Asn Leu Tyr Gln Trp Asn Ala Glu Tyr Ser Arg Leu Leu Leu Asp 
705                 710                 715                 720 


Val Met Lys Glu Asn Lys Gly Lys Asn Ile Gln Asn Thr Phe Arg Ala 
                725                 730                 735     


Glu Ile Glu Glu Leu Ile Cys Gly Lys Phe Gly Ile Arg Leu Gly Ser 
            740                 745                 750         


Leu Phe His His Ser Leu Gln Phe Leu Thr Asn Cys Lys Ser Leu Ile 
        755                 760                 765             


Ser Ser Tyr Phe Met Leu Asn Asn Lys Lys Glu Glu Tyr Asp Gln Glu 
    770                 775                 780                 


Leu Phe Asp Ser Asp Phe Phe Arg Leu Met Lys Ser Ile Gly Asp Lys 
785                 790                 795                 800 


Arg Val Arg Lys Arg Lys Glu Lys Ser Ser Arg Ile Ser Ser Thr Val 
                805                 810                 815     


Leu Gln Ile Ala Arg Glu Asn Asn Val Lys Ser Leu Cys Val Glu Gly 
            820                 825                 830         


Tyr Leu Pro Thr Ser Thr Lys Lys Thr Lys Pro Lys Gln Asn Gln Lys 
        835                 840                 845             


Ser Ile Asp Trp Cys Ala Arg Ala Val Val Lys Lys Leu Asn Asp Gly 
    850                 855                 860                 


Cys Lys Val Leu Gly Ile Tyr Leu Gln Ala Ile Asp Pro Arg Asp Thr 
865                 870                 875                 880 


Ser His Leu Asp Pro Phe Val Tyr Tyr Gly Lys Lys Ser Thr Lys Val 
                885                 890                 895     


Gly Lys Glu Ala Arg His Thr Ile Val Glu Pro Ser Asn Ile Lys Glu 
            900                 905                 910         


Tyr Met Thr Asn Arg Phe Asp Asp Trp His Arg Gly Val Thr Lys Lys 
        915                 920                 925             


Ser Lys Lys Gly Asp Val Gln Thr Ser Thr Thr Val Leu Leu Tyr Gln 
    930                 935                 940                 


Glu Ala Leu Arg Gln Phe Ala Ser His Tyr Lys Leu Asp Phe Asp Ser 
945                 950                 955                 960 


Leu Pro Lys Met Lys Phe Tyr Glu Leu Ala Lys Ile Leu Gly Asp His 
                965                 970                 975     


Glu Lys Val Ile Ile Pro Cys Arg Gly Gly Arg Ala Tyr Leu Ser Thr 
            980                 985                 990         


Tyr Pro Val Thr Lys Asp Ser Ser  Lys Ile Thr Phe Asn  Gly Arg Glu 
        995                 1000                 1005             


Arg Trp  Tyr Asn Glu Ser Asp  Val Val Ala Ala Val  Asn Ile Val 
    1010                 1015                 1020             


Leu Arg  Gly Ile Ile Asp Glu  Asp Glu Gln Pro Asp  Gly Ala Lys 
    1025                 1030                 1035             


Lys Gln  Ala Thr Thr Arg Arg  Thr Ser Arg Ala Asp  Pro Lys Lys 
    1040                 1045                 1050             


Lys Arg  Lys Val 
    1055         


<210>  22
<211>  1044
<212>  PRT
<213>  ˹

<220>
<223>  Cas12i.3-NLSںϵ׵İ

<400>  22

Met Met Ser Asp Asn Ile Ile Leu Pro Tyr Asn Ser Lys Leu Ala Pro 
1               5                   10                  15      


Asp Glu Arg Lys Gln Arg Leu Leu Asn Asp Thr Phe Asn Trp Phe Asp 
            20                  25                  30          


Met Cys Asn Glu Val Phe Phe Asp Phe Val Lys Asn Leu Tyr Gly Gly 
        35                  40                  45              


Val Lys His Glu His Leu Ile Leu Val Asn Phe Ala Glu Lys Pro Lys 
    50                  55                  60                  


Lys Val Ser Asn Ser Lys Lys Pro Lys Lys Lys Asp Gln Glu Val Asn 
65                  70                  75                  80  


Ile His Val Glu Pro Asn Gln Ala Glu Trp Val Asp Asn Ala Cys Ala 
                85                  90                  95      


Thr Phe Trp Phe Arg Leu Gln Ala Lys Ser Thr Val Gln Leu Asp Gln 
            100                 105                 110         


Ser Val Gln Thr Ala Glu Glu Arg Ile Arg Arg Phe Arg Asp Tyr Ala 
        115                 120                 125             


Gly His Glu Pro Ser Ser Phe Ala Lys Ser Tyr Leu Asn Gly Asn Tyr 
    130                 135                 140                 


Asp Pro Glu Lys Thr Glu Trp Val Asp Cys Arg Leu Leu Tyr Val Asn 
145                 150                 155                 160 


Phe Cys Arg Asn Leu Asn Val Asn Leu Asp Ala Asp Ile Arg Thr Met 
                165                 170                 175     


Val Glu His Asn Leu Leu Pro Val Leu Pro Gly Gln Asp Phe Lys Thr 
            180                 185                 190         


Asn Asn Val Phe Ser Asn Ile Phe Gly Val Gly Asn Lys Glu Asp Lys 
        195                 200                 205             


Gly Gln Lys Thr Asn Trp Leu Asn Thr Val Ser Glu Gly Leu Gln Ser 
    210                 215                 220                 


Lys Glu Ile Trp Asn Trp Asp Glu Tyr Arg Asp Leu Ile Ser Arg Ser 
225                 230                 235                 240 


Thr Gly Cys Ser Thr Ala Ala Glu Leu Arg Ser Glu Ser Ile Gly Arg 
                245                 250                 255     


Pro Ser Met Leu Ala Val Asp Phe Ala Ser Glu Lys Ser Gly Gln Ile 
            260                 265                 270         


Ser Gln Glu Trp Leu Ala Glu Arg Val Lys Ser Phe Arg Ala Ala Ala 
        275                 280                 285             


Ser Gln Lys Ser Lys Ile Tyr Asp Met Pro Asn Arg Leu Val Leu Lys 
    290                 295                 300                 


Glu Tyr Ile Ala Ser Lys Ile Gly Pro Phe Lys Leu Glu Arg Trp Ser 
305                 310                 315                 320 


Ala Ala Ala Val Ser Ala Tyr Lys Asp Val Arg Ser Lys Asn Ser Ile 
                325                 330                 335     


Asn Leu Leu Tyr Ser Lys Glu Arg Leu Trp Arg Cys Lys Glu Ile Ala 
            340                 345                 350         


Gln Ile Leu Val Asp Asn Thr Gln Val Ala Glu Ala Gln Gln Ile Leu 
        355                 360                 365             


Val Asn Tyr Ser Ser Gly Asp Thr Asn Ser Phe Thr Val Glu Asn Arg 
    370                 375                 380                 


His Met Gly Asp Leu Thr Val Leu Phe Lys Ile Trp Glu Lys Met Asp 
385                 390                 395                 400 


Met Asp Ser Gly Ile Glu Gln Tyr Ser Glu Ile Tyr Arg Asp Glu Tyr 
                405                 410                 415     


Ser Arg Asp Pro Ile Thr Glu Leu Leu Arg Tyr Leu Tyr Asn His Arg 
            420                 425                 430         


His Ile Ser Ala Lys Thr Phe Arg Ala Ala Ala Arg Leu Asn Ser Leu 
        435                 440                 445             


Leu Leu Lys Asn Asp Arg Lys Lys Ile His Pro Thr Ile Ser Gly Arg 
    450                 455                 460                 


Thr Ser Val Ser Phe Gly His Ser Thr Ile Lys Gly Cys Ile Thr Pro 
465                 470                 475                 480 


Pro Asp His Ile Val Lys Asn Arg Lys Glu Asn Ala Gly Ser Thr Gly 
                485                 490                 495     


Met Ile Trp Val Thr Met Gln Leu Ile Asp Asn Gly Arg Trp Ala Asp 
            500                 505                 510         


His His Ile Pro Phe His Asn Ser Arg Tyr Tyr Arg Asp Phe Tyr Ala 
        515                 520                 525             


Tyr Arg Ala Asp Leu Pro Thr Ile Ser Asp Pro Arg Arg Lys Ser Phe 
    530                 535                 540                 


Gly His Arg Ile Gly Asn Asn Ile Ser Asp Thr Arg Met Ile Asn His 
545                 550                 555                 560 


Asp Cys Lys Lys Ala Ser Lys Met Tyr Leu Arg Thr Ile Gln Asn Met 
                565                 570                 575     


Thr His Asn Val Ala Phe Asp Gln Gln Thr Gln Phe Ala Val Arg Arg 
            580                 585                 590         


Tyr Ala Asp Asn Asn Phe Thr Ile Thr Ile Gln Ala Arg Val Val Gly 
        595                 600                 605             


Arg Lys Tyr Lys Lys Glu Ile Ser Val Gly Asp Arg Val Met Gly Val 
    610                 615                 620                 


Asp Gln Asn Gln Thr Thr Ser Asn Thr Tyr Ser Val Trp Glu Val Val 
625                 630                 635                 640 


Ala Glu Gly Thr Glu Asn Ser Tyr Pro Tyr Lys Gly Asn Asn Tyr Arg 
                645                 650                 655     


Leu Val Glu Asp Gly Phe Ile Arg Ser Glu Cys Ser Gly Arg Asp Gln 
            660                 665                 670         


Leu Ser Tyr Asp Gly Leu Asp Phe Gln Asp Phe Ala Gln Trp Arg Arg 
        675                 680                 685             


Glu Arg Tyr Ala Phe Leu Ser Ser Val Gly Cys Ile Leu Asn Asp Glu 
    690                 695                 700                 


Ile Glu Pro Gln Ile Pro Val Ser Ala Glu Lys Ala Lys Lys Lys Lys 
705                 710                 715                 720 


Lys Phe Ser Lys Trp Arg Gly Cys Ser Leu Tyr Ser Trp Asn Leu Cys 
                725                 730                 735     


Tyr Ala Tyr Tyr Leu Lys Gly Leu Met His Glu Asn Leu Ala Asn Asn 
            740                 745                 750         


Pro Ala Gly Phe Arg Gln Glu Ile Leu Asn Phe Ile Gln Gly Ser Arg 
        755                 760                 765             


Gly Val Arg Leu Cys Ser Leu Asn His Thr Ser Phe Arg Leu Leu Ser 
    770                 775                 780                 


Lys Ala Lys Ser Leu Ile His Ser Phe Phe Gly Leu Asn Asn Ile Lys 
785                 790                 795                 800 


Asp Pro Glu Ser Gln Arg Asp Phe Asp Pro Glu Ile Tyr Asp Ile Met 
                805                 810                 815     


Val Asn Leu Thr Gln Arg Lys Thr Asn Lys Arg Lys Glu Lys Ala Asn 
            820                 825                 830         


Arg Ile Thr Ser Ser Ile Leu Gln Ile Ala Asn Arg Leu Asn Val Ser 
        835                 840                 845             


Arg Ile Val Ile Glu Asn Asp Leu Pro Asn Ala Ser Ser Lys Asn Lys 
    850                 855                 860                 


Ala Ser Ala Asn Gln Arg Ala Thr Asp Trp Cys Ala Arg Asn Val Ser 
865                 870                 875                 880 


Glu Lys Leu Glu Tyr Ala Cys Lys Met Leu Gly Ile Ser Leu Trp Gln 
                885                 890                 895     


Ile Asp Pro Arg Asp Thr Ser His Leu Asp Pro Phe Val Val Gly Lys 
            900                 905                 910         


Glu Ala Arg Phe Met Lys Ile Lys Val Ser Asp Ile Asn Glu Tyr Thr 
        915                 920                 925             


Ile Ser Asn Phe Lys Lys Trp His Ala Asn Ile Ala Thr Thr Ser Thr 
    930                 935                 940                 


Thr Ala Pro Leu Tyr His Asp Ala Leu Lys Ala Phe Ser Ser His Tyr 
945                 950                 955                 960 


Gly Ile Asp Trp Asp Asn Leu Pro Glu Met Lys Phe Trp Glu Leu Lys 
                965                 970                 975     


Asn Ala Leu Lys Asp His Lys Glu Val Phe Ile Pro Asn Arg Gly Gly 
            980                 985                 990         


Arg Cys Tyr Leu Ser Thr Leu Pro  Val Thr Ser Thr Ser  Glu Lys Ile 
        995                 1000                 1005             


Val Phe  Asn Gly Arg Glu Arg  Trp Leu Asn Ala Ser  Asp Ile Val 
    1010                 1015                 1020             


Ala Gly  Val Asn Ile Val Leu  Arg Ser Val Ser Arg  Ala Asp Pro 
    1025                 1030                 1035             


Lys Lys  Lys Arg Lys Val 
    1040                 


<210>  23
<211>  3713
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.1ϵͳеĺ

<400>  23
tttacacttt atgcttccgg ctcgtatgtt aggaggtctt tatcatgtct aacaaagaaa       60

aaaatgcaag cgaaactcgc aaagcctaca caacaaaaat gattccaaga agccatgatc      120

gcatgaaatt gcttgggaat ttcatggatt atttgatgga tggaacgcca atatttttcg      180

aactttggaa tcagtttggc ggcgggattg accgcgatat catttctggc actgcaaata      240

aagacaagat atcagatgat ttacttttgg cggtcaattg gttcaaggta atgccaatta      300

attctaagcc tcaaggtgta tcgccatcaa atcttgccaa cctctttcaa caatactctg      360

gatcagaacc agacattcaa gctcaagagt attttgcttc aaattttgac accgaaaagc      420

atcaatggaa ggacatgcgt gttgaatacg aacgactatt agctgaattg cagctatcga      480

gaagtgatat gcatcatgac ttgaagctca tgtacaaaga aaaatgcatt ggcctaagtc      540

tttctacggc tcactacatc acttctgtga tgtttgggac aggagctaaa aacaatcgcc      600

aaaccaagca tcaattctat agcaaggtta tccaactact tgaggaatca actcaaatca      660

attctgttga acagttggca tctattattt tgaaagcagg agattgcgat agttatcgaa      720

agcttcgtat tcgatgttct cgtaagggag caacacccag cattcttaag atcgttcaag      780

actatgaact gggaaccaat cacgatgatg aagtgaatgt gccaagtttg attgcaaatt      840

tgaaagaaaa attgggcaga tttgaatatg aatgcgaatg gaagtgcatg gaaaaaatca      900

aagcattttt agctagcaaa gttgggcctt attacctagg ctcttacagt gcgatgcttg      960

aaaatgcatt gtcgcccatc aagggaatga ctacaaaaaa ttgcaaattt gtgttaaagc     1020

aaattgatgc caaaaacgac atcaagtatg aaaatgagcc atttggcaaa attgttgaag     1080

ggttttttga ctctccatat tttgaaagcg acaccaatgt gaaatgggtt ttgcacccac     1140

atcatattgg agaaagcaat atcaaaacac tctgggaaga cttgaatgca attcattcta     1200

agtacgaaga agatattgct tctttgagcg aagacaaaaa agagaaacgc attaaggttt     1260

atcaaggaga tgtttgccaa acaatcaata cgtattgtga agaagtagga aaggaagcta     1320

agactccttt agttcagctt ttgcgttatc tttactctag aaaagatgat attgctgttg     1380

ataagataat tgatggcatt accttcctta gcaagaaaca caaggttgaa aaacaaaaaa     1440

tcaatcctgt aattcaaaaa tatcccagtt tcaactttgg gaataattct aagttgttgg     1500

gaaagattat cagccccaaa gacaagttaa agcataatct caaatgcaac aggaatcagg     1560

ttgataatta catttggatt gagattaaag tactaaacac caaaacgatg cgatgggaaa     1620

agcatcacta tgctttatca tctacgcggt ttttggaaga ggtctattat ccagccacat     1680

ccgaaaatcc gccagacgct ttggcagcac gtttccgaac taaaactaat gggtatgaag     1740

gcaagcctgc gttgtctgct gagcaaattg aacaaattag atcagcccca gtcggtttga     1800

gaaaagtgaa aaaacgtcaa atgcgactcg aagctgcaag acagcaaaat ctcttgcctc     1860

gatacacttg gggcaaagat ttcaacataa acatttgtaa gcgtggcaac aattttgaag     1920

tcactcttgc gacgaaggtg aaaaagaaaa aagaaaagaa ttataaggtt gttttagggt     1980

acgatgctaa tatcgttcgc aaaaacactt acgcagccat agaagctcac gctaatggcg     2040

atggtgtgat tgactacaat gacttgcccg tgaagcctat tgaaagtgga tttgtaaccg     2100

ttgaaagtca agtgcgagac aaatcttacg atcaactctc ttacaatggc gtaaagctct     2160

tgtattgcaa gcctcatgtt gagtctcgac gttcattttt ggagaaatac cgaaatggca     2220

ccatgaagga caacagagga aacaacattc aaattgactt tatgaaagac tttgaagcta     2280

ttgcggatga tgaaacttct ttgtattact tcaatatgaa gtactgcaag ctgcttcaat     2340

cgtccattcg caatcattct tcacaagcaa aagaatatcg tgaagagatt tttgaattgt     2400

taagagacgg aaaactatcg gttttgaagt tatcatcttt gagcaatctt tcttttgtga     2460

tgttcaaagt tgccaaatct ctgatcggta cttactttgg ccacttgctt aagaagccga     2520

agaattctaa gtcagatgtt aaggcaccgc ctataactga tgaagataag caaaaagctg     2580

atcctgagat gtttgctttg aggttggctt tggaggagaa gcgactaaac aaagtcaagt     2640

ctaagaaaga agtaattgcg aacaagattg ttgctaaggc acttgagctt cgcgacaagt     2700

acgggcctgt gttgattaag ggagaaaaca tctctgacac gaccaagaaa ggcaagaagt     2760

caagcaccaa ttcttttttg atggactggc tagcacgcgg tgtggctaat aaagtcaaag     2820

aaatggtaat gatgcatcaa ggacttgaat ttgtagaagt aaatcctaat ttcacatctc     2880

accaagatcc ttttgttcac aagaaccctg aaaatacgtt tagagctagg tacagtcggt     2940

gcactccaag tgaacttact gagaaaaatc gcaaggaaat tttgagcttt ttgagcgata     3000

agccttctaa acgaccgaca aatgcctatt acaatgaagg tgcgatggcc tttcttgcaa     3060

cttatggctt gaagaagaat gatgtgctag gagttagtct tgagaaattc aagcaaataa     3120

tggccaacat tctacatcag cgttccgaag atcaattatt gtttccttct agaggtggca     3180

tgttttatct tgcaacttac aagcttgatg ctgacgctac ctctgtaaat tggaatggca     3240

aacagttttg ggtttgtaac gcagatttag tagcggcata caatgtcggt ttggtcgata     3300

ttcaaaaaga cttcaagaaa aagtaaaaat aaaacgaaag gctcagtcga aagactgggc     3360

ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atttgacagc     3420

tagctcagtc ctaggtataa tgctagcgct gacgttggaa tgactaattt ttgtgcccac     3480

cgttggcacg gtataacaac ttcgacgagc tctacacgtt ggaatgacta atttttgtgc     3540

ccaccgttgg cacggatcgc tgagaccgca tcaaagcacg atgagcgtgg cgttggaatg     3600

actaattttt gtgcccaccg ttggcacaaa taaaacgaaa ggctcagtcg aaagactggg     3660

cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aat            3713


<210>  24
<211>  3566
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.2ϵͳеĺ

<400>  24
tttacacttt atgcttccgg ctcgtatgtt aggaggtctt tatcatggtc agcgaaagta       60

cgatccgtcc ttataccagc aaattggcac caaatgattc aaagctgaaa atgcttaacg      120

atacattcaa ttggctagac catgcataca aggtattctt tgatgtatca gtagcacttt      180

ttggtgccat tgaacatgaa actgctcaag aactgatagg tgaaaaaagt aaattcgacg      240

cagatctact ctgtgctatc atgtggtttc gcctagaaga aaaatcagat aaccccggac      300

ctctccagac agtagaacaa aggatgagac tattccagaa atactctgga cacgaaccat      360

cttctttcac acaagaatac atcaaaggaa acatagattc agaaaaatac caatgggtag      420

attgtcgtct aaaatttata gacttagcta gaaatattaa cacaactcaa gaatcactca      480

aaattgatgc atacactctc ttcatgaata aattaattcc tgtgagcaaa gatgatgaat      540

tcaacgctta tggcttgatt tcacaacttt ttggaacagg aaagaaagaa gaccgatcaa      600

tcaaagcatc aatgcttgaa gaaatctcaa atattctcgc agacaaaaat ccaaacactt      660

gggaagaata tcaggattta attaaaaaaa ctttcaatgt tgataattac aaagaactta      720

aagaaaaatt aagcgcagga agcagtggtc gtgatggatc tctagtcatt gacctcaaag      780

aagaaaaaac aggattactt caacctaatt ttatcaaaaa tcgtattgtt aaattcagag      840

aagatgctga caagaagaga actgtatttc tattgcccaa tagaatgaag ttgagagaat      900

ttattgcttc gcaaattgga ccatttgaac aaaatagttg gtcggctgtt ctaaacagat      960

ctatggccgc aatccaatca aaaaatagca gcaacattct atacactaat gaaaaagaag     1020

aacgcaataa tgaaattcaa gaattgttga aaaaagacat cttgtcagca gcaagtatat     1080

taggcgattt tcgtcgtgga gaatttaaca gatcagtggt ttcaaaaaat cacttgggag     1140

caagactcaa tgagcttttt gaaatatggc aagaattaac aatggatgat ggaatcaaaa     1200

aatatgttga tctttgtaaa gataagtttt ccagaagacc tgtaaaggca ttgcttcaat     1260

acatttatcc atatttcgat aaaattaatg caaagcaatt tcttgatgca gctagttaca     1320

acacacttgt tgaaaccaat aatcgcaaga agattcaccc aactgtcaca ggaccaacag     1380

tttgtaattg gggaccgaag tctacaatta atggatcaat aacaccacca aatcaaatgg     1440

tcaaaggaag accagcagga tctcatggaa tgatttgggt cacaatgaca gtcatagata     1500

atggacgttg gatcaagcat caccttccat tccataactc acgttattac gaagaacact     1560

attgctacag agaaggtttg cctacaaaaa ataaacctcg tactaaacaa cttggtactc     1620

aagtaggatc aacaatttcc gctccaagtc ttgctattct taaatctcaa gaagaacaag     1680

atagaaggaa tgatcgtaaa aatagattca aagcccacaa atctatcatc agatcacaag     1740

agaacattga atacaatgtt gcctttgaca agtcaactaa ttttgacgta acacgaaaaa     1800

atggtgagtt tttcatcact atctcttcta gagttgctac tccaaaatat agttataaat     1860

taaatattgg cgatatgatc atgggactgg acaacaacca aacagcccca tgcacatatt     1920

caatttggcg tgtagtggag aaagatacag aaggtagttt ctttcataat aaaatttggc     1980

tccaattggt aactgacggt aaagtaacaa gtattgttga caataaccgt caagtcgatc     2040

agctttctta tgctggtatt gaatactcca attttgctga atggagaaaa gatcgtcgcc     2100

aattccttcg atcaattaac gaagattacg ttaaaaaatc agataattgg cgtaatatga     2160

atctttatca atggaatgct gaatattctc gtttgcttct tgatgtcatg aaggaaaata     2220

agggcaaaaa tattcaaaat acattccgtg cagaaataga agaattaatt tgtggtaagt     2280

tcggtataag attgggaagt ctttttcatc attcccttca atttcttact aattgtaaga     2340

gtcttatatc atcttatttt atgcttaaca ataaaaaaga agagtatgat caagagttgt     2400

ttgacagtga tttctttagg ttgatgaaaa gtattgggga caaacgtgtt aggaaacgca     2460

aagagaaatc ttcaaggatt tcatctacag tattgcaaat tgcgagggaa aataatgtca     2520

agtctttgtg tgtcgagggt tatttgccta catccacaaa gaagactaag ccaaaacaaa     2580

atcaaaaatc aatagattgg tgtgctcgtg ctgttgttaa aaaattgaat gatggttgta     2640

aggttttggg tatttatcta caggctattg atccaagaga tacgagtcat ttagatccat     2700

ttgtctatta tggaaagaaa tctactaaag ttggaaaaga agctcgacac acaattgttg     2760

agccatccaa tataaaggaa tacatgacaa acagattcga tgactggcat cgaggtgtta     2820

ccaaaaagtc aaaaaagggt gatgttcaaa caagcactac tgttcttctt tatcaagaag     2880

ctttaaggca atttgctagc cattacaaac ttgattttga ctctttgcca aaaatgaaat     2940

tctatgaatt agctaaaata ttgggagatc atgaaaaagt gattatccct tgtcgtggag     3000

gaagagctta tctttctact tatccagtaa caaaagattc ctcgaaaata actttcaatg     3060

gtagagaaag atggtataat gaatcagatg tggtagctgc tgttaacatc gtgctgagag     3120

gcataataga tgaagacgag cagcctgatg gtgccaaaaa acaggcaacc actcgcagaa     3180

cgtaaaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt     3240

gtcggtgaac gctctcctga gtaggacaaa tttgacagct agctcagtcc taggtataat     3300

gctagcgctg accacaatac ctgagaaatc cgtcctacgt tgacggggta taacaacttc     3360

gacgagctct acaccacaat acctgagaaa tccgtcctac gttgacgggg atcgctgaga     3420

ccgcatcaaa gcacgatgag cgtggccaca atacctgaga aatccgtcct acgttgacgg     3480

aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg     3540

tgaacgctct cctgagtagg acaaat                                          3566


<210>  25
<211>  3530
<212>  DNA
<213>  ˹

<220>
<223>  Cas12i.3ϵͳеĺ

<400>  25
tttacacttt atgcttccgg ctcgtatgtt aggaggtctt tatcatgatg tctgataata       60

ttattctgcc ttacaactct aaacttgccc ccgatgagcg taagcaaagg cttctgaacg      120

acacattcaa ttggtttgat atgtgtaacg aagttttttt tgacttcgtg aagaatctgt      180

atggcggagt gaagcatgaa catttgattc ttgtgaattt tgccgagaaa cccaagaagg      240

ttagcaatag taaaaagcct aagaaaaaag atcaggaagt caacattcat gttgagccca      300

atcaagccga atgggttgac aatgcttgtg ccacattctg gtttcgattg caggcaaaat      360

caacagtaca attagaccaa tcagtccaga cagcagaaga gcgtattcga cgatttcggg      420

attatgctgg tcacgagcca tcatcatttg ccaaatctta tctaaatggt aattatgatc      480

cagagaagac tgaatgggtt gattgcaggc ttctttatgt caatttttgc cgtaatctga      540

atgtcaatct tgatgcggac attcgcacaa tggtcgaaca caatcttctt cctgttctcc      600

ccggtcagga tttcaaaacc aacaatgtat tctccaacat ttttggagta ggcaataagg      660

aagacaaggg tcaaaaaacg aactggctta acacggtctc agaaggcctt cagtccaagg      720

aaatttggaa ttgggatgag tatcgtgatt tgatatcaag atctactgga tgctctacgg      780

cagcagaact gaggtctgag tcgattggca ggccaagcat gcttgcagtt gattttgcat      840

ctgagaaatc aggccaaata tcacaggaat ggcttgcaga aagggttaag tcttttaggg      900

cagcagcttc tcaaaaaagc aaaatctatg acatgcctaa tcgtcttgtt ttgaaggaat      960

acattgcttc aaaaatcggg cctttcaaac ttgaaaggtg gtctgctgct gccgtttctg     1020

cttataagga tgtccgtagc aagaatagta tcaatttgct ttattccaag gaaagattgt     1080

ggcgatgcaa ggaaattgct cagattttgg ttgataatac gcaggttgct gaagcccaac     1140

agattcttgt taattattct tctggtgata ccaattcatt cacagttgaa aatcgtcaca     1200

tgggcgattt gactgttctt ttcaagattt gggaaaagat ggatatggat tctggcatag     1260

aacagtattc cgaaatttat cgtgatgaat atagtcgtga tccaattaca gagttgctac     1320

gctatctcta caatcatagg catatttcgg caaaaacttt tagggctgct gcaaggttga     1380

attctctttt gctgaagaat gatcgcaaga aaattcaccc gactatcagt ggtaggacta     1440

gcgtttcttt cggccattca acaatcaagg gatgcattac tcctcccgat catattgtca     1500

aaaatcgaaa agagaatgct ggaagcactg gcatgatctg ggttacaatg cagcttatcg     1560

ataatggtcg atgggcggat catcatattc ctttccacaa ttctcgctac tatcgtgatt     1620

tttatgccta tcgtgccgat ctaccgacta tttctgatcc tcgtcgtaaa tcttttggac     1680

ataggatcgg caataatatt agcgatacaa ggatgattaa tcatgattgc aaaaaagcat     1740

caaaaatgta tcttcgcaca atccagaata tgacgcacaa tgtggcattc gaccagcaga     1800

ctcagttcgc tgttcgtcgt tatgctgata ataatttcac gattacgatc caagctaggg     1860

ttgtagggag gaaatataaa aaggaaatat cagttggtga tcgtgtgatg ggtgttgacc     1920

agaatcagac cacaagtaac acatattctg tttgggaagt agttgctgaa gggactgaaa     1980

attcttaccc atataagggc aataattatc gtctcgttga ggatggattt attcgcagcg     2040

aatgcagtgg tcgagatcag ctttcctatg atggtcttga ttttcaggac tttgctcaat     2100

ggcgcaggga aagatacgca tttttatctt ctgttggatg tattctcaat gacgaaatcg     2160

agcctcaaat ccctgttagt gccgaaaagg caaaaaagaa gaagaagttt tctaagtggc     2220

gtggttgttc tctttatagt tggaacctct gttatgcata ttatcttaag ggtttgatgc     2280

atgagaactt ggcaaataat cctgctggat tccgacagga aattctgaat tttattcagg     2340

gttctagggg cgtgaggctt tgttccctta atcacactag cttccgactt ctctctaaag     2400

ccaagtctct aattcattca ttttttggtc taaacaatat caaagatcct gaatctcaaa     2460

gggattttga tccagaaatt tatgacataa tggtcaatct gacacaaagg aagaccaaca     2520

agaggaagga aaaagctaat cgcatcactt cttcaatttt gcaaattgcc aataggttga     2580

atgtcagtcg cattgtgatt gagaacgatt tgccgaatgc aagttccaaa aacaaggcat     2640

cagccaatca aagggcaact gattggtgtg ctagaaatgt atctgagaaa ttagaatatg     2700

cctgcaagat gcttggtatc agcttatggc agattgatcc aagggacaca tcgcaccttg     2760

atccatttgt tgtgggcaag gaggctagat ttatgaaaat aaaggtttct gatattaacg     2820

aatacactat cagtaatttc aaaaagtggc atgcaaacat tgctacaaca agtactacag     2880

cacctcttta tcatgatgct ttgaaggcat tctcttctca ttatggaatt gattgggaca     2940

atttgccaga aatgaagttt tgggaattga agaatgcctt gaaagaccat aaagaggtgt     3000

ttatcccaaa tcgtggtggt cgctgctatt tatcgacatt gccggtgact tctacatctg     3060

aaaagattgt tttcaatgga agagagagat ggttgaacgc aagtgatatt gtggcaggag     3120

ttaacattgt gctaagatca gtatgaaaat aaaacgaaag gctcagtcga aagactgggc     3180

ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atttgacagc     3240

tagctcagtc ctaggtataa tgctagcgct gactcgcaat gccttagaaa tccgtccttg     3300

gttgacgggg tataacaact tcgacgagct ctacactcgc aatgccttag aaatccgtcc     3360

ttggttgacg gggatcgctg agaccgcatc aaagcacgat gagcgtggct cgcaatgcct     3420

tagaaatccg tccttggttg acggaaataa aacgaaaggc tcagtcgaaa gactgggcct     3480

ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat                3530


<210>  26
<211>  35
<212>  DNA
<213>  ˹

<220>
<223>  PAMĿ


<220>
<221>  misc_feature
<222>  (1)..(8)

<220>
<221>  misc_feature
<222>  (1)..(8)
<223>  n = agct

<400>  26
nnnnnnnngg tataacaact tcgacgagct ctaca                                  35


<210>  27
<211>  45
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.1RNA

<400>  27
auuuuugugc ccaccguugg cacgguggug gucaccauau aucuc                       45


<210>  28
<211>  45
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.2RNA

<400>  28
agaaauccgu ccuacguuga cgggguggug gucaccauau aucuc                       45


<210>  29
<211>  45
<212>  RNA
<213>  ˹

<220>
<223>  Cas12i.3RNA

<400>  29
agaaauccgu ccuugguuga cgggguggug gucaccauau aucuc                       45


<210>  30
<211>  27
<212>  DNA
<213>  ˹

<220>
<223>  

<400>  30
ggtataacaa cttcgacgag ctctaca                                           27


