                         SEQUENCE LISTING

<110>  Biosion Inc.
 
<120>  ANTIBODIES BINDING PD-1 AND USES THEREOF

<130>  55559 00001

<150>  US62/657927
<151>  2018-04-15

<150>  US62/795573
<151>  2019-01-23

<160>  68    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 for mouse, chimeric and humanized D2H3, defined by IMGT 
       numbering scheme

<400>  1

Gly Tyr Thr Phe Thr Asn Tyr Trp 
1               5               


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 for mouse, chimeric and humanized D2H3, defined by IMGT 
       numbering scheme

<400>  2

Ile Phe Pro Arg Asn Ser Glu Thr 
1               5               


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 for mouse, chimeric and humanized D2H3,  defined by IMGT 
       numbering scheme

<400>  3

Thr Arg Asn Arg Tyr Gly Leu Asp Tyr 
1               5                   


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 for mouse, chimeric and humanized D2A4, defined by IMGT 
       numbering scheme

<400>  4

Gly Phe Thr Phe Ser Ser Tyr Thr 
1               5               


<210>  5
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 for mouse, chimeric and humanized D2A4, defined by IMGT 
       numbering scheme

<400>  5

Ile Ser Gly Gly Gly Ser Asn Thr 
1               5               


<210>  6
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 for mouse, chimeric and humanized D2A4, defined by IMGT 
       numbering scheme

<400>  6

Ala Arg Gln Ala Phe Tyr Ser Asn Tyr Trp Tyr Phe Asp Val 
1               5                   10                  


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 for mouse, chimeric and humanized D2H3,  defined by 
       Chothia or Kabat numbering scheme

<400>  7

Arg Ala Ser Glu Ser Val Ser Leu His Gly Thr Arg Leu Met His 
1               5                   10                  15  


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR2 for mouse, chimeric and humanized D2H3, defined by 
       Chothia or Kabat numbering scheme

<400>  8

Leu Gly Ser Asn Leu Glu Ser 
1               5           


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL -CDR3 for mouse, chimeric and humanized D2H3, defined by IMGT 
       numbering scheme, Chothia numbering scheme or Kabat numbering 
       scheme

<400>  9

Gln Gln Ser Ile Glu Asp Pro Trp Thr 
1               5                   


<210>  10
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 for mouse, chimeric and humanized D2A4, defined by 
       Chothia or Kabat numbering scheme

<400>  10

Leu Ala Ser Gln Thr Ile Gly Thr Trp Leu Ala 
1               5                   10      


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL -CDR2 for mouse, chimeric and humanized D2A4, defined by 
       Chothia or Kabat numbering scheme

<400>  11

Ala Ala Thr Ser Leu Ala Asp 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL -CDR3 for mouse, chimeric and humanized D2A4,  defined by IMGT
       numbering scheme, Chothia numbering scheme or Kabat numbering 
       scheme

<400>  12

Gln Gln Val Ser Ser Ile Pro Trp Thr 
1               5                   


<210>  13
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for mouse D2H3

<400>  13

Glu Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ser Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Lys Ala Lys Leu Thr Ala Val Thr Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Val Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Ser Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  14
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V1, huD2H3-V8- huD2H3-V11 and huD2H3-V13

<400>  14

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Arg Ala Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  15
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V2

<400>  15

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Arg Ala Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  16
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V3

<400>  16

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Arg Ala Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  17
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V4

<400>  17

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Arg Val Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  18
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V5

<400>  18

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Arg Ala Thr Ile Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  19
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V6

<400>  19

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Arg Ala Thr Leu Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  20
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V7

<400>  20

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ser Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Arg Ala Thr Leu Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  21
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V12 and huD2H3-V14

<400>  21

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Ala Arg Val Thr Ile Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Asn Arg Tyr Gly Leu Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  22
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for mouse D2A4

<400>  22

Glu Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Thr Met Ser Trp Val Arg Gln Thr Pro Ala Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Gly Gly Gly Ser Asn Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Ala Phe Tyr Ser Asn Tyr Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  23
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2A4-V1 and huD2A4-V4

<400>  23

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Thr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Gly Gly Gly Ser Asn Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Ala Phe Tyr Ser Asn Tyr Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  24
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2A4-V2

<400>  24

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Thr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Gly Gly Gly Ser Asn Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Ala Phe Tyr Ser Asn Tyr Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Ala Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  25
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2A4-V3

<400>  25

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Thr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Ser Gly Gly Gly Ser Asn Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Ala Phe Tyr Ser Asn Tyr Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Lys Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  26
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH for huD2A4-V5 and huD2A4-V6

<400>  26

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Thr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Ser Gly Gly Gly Ser Asn Thr Tyr Tyr Pro Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gln Ala Phe Tyr Ser Asn Tyr Trp Tyr Phe Asp Val Trp Gly 
            100                 105                 110         


Lys Gly Thr Thr Val Thr Val Ser Ser 
        115                 120     


<210>  27
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for mouse D2H3

<400>  27

Asp Ile Val Leu Thr Gln Ser Pro Gly Phe Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Ser Leu His 
            20                  25                  30          


Gly Thr Arg Leu Met His Trp Tyr His Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Ser Leu Gly Ser Asn Leu Glu Ser Gly Val Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Glu Thr Asp Phe Thr Leu Asn Ile His 
65                  70                  75                  80  


Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Phe Cys Gln Gln Ser Ile 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  28
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huD2H3-V1 - huD2H3-V7 and huD2H3-V12

<400>  28

Asp Ile Val Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Ser Leu His 
            20                  25                  30          


Gly Thr Arg Leu Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Ser Leu Gly Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Glu Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln Ser Ile 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  29
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huD2H3-V8

<400>  29

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Ser Leu His 
            20                  25                  30          


Gly Thr Arg Leu Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Ser Leu Gly Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Glu Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln Ser Ile 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  30
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huD2H3-V9

<400>  30

Asp Ile Val Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Ser Leu His 
            20                  25                  30          


Gly Thr Arg Leu Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Gly Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Glu Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln Ser Ile 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  31
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huD2H3-V10

<400>  31

Asp Ile Val Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Ser Leu His 
            20                  25                  30          


Gly Thr Arg Leu Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Ser Leu Gly Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Phe Cys Gln Gln Ser Ile 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  32
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huD2H3-V11

<400>  32

Asp Ile Val Leu Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Ser Leu His 
            20                  25                  30          


Gly Thr Arg Leu Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Ser Leu Gly Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Glu Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Ile 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  33
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huD2H3-V13 and huD2H3-V14

<400>  33

Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Glu Arg Ala Thr Ile Asn Cys Arg Ala Ser Glu Ser Val Ser Leu His 
            20                  25                  30          


Gly Thr Arg Leu Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Leu Gly Ser Asn Leu Glu Ser Gly Val Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln Ser Ile 
                85                  90                  95      


Glu Asp Pro Trp Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  34
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for mouse D2A4

<400>  34

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Gln Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Ser Val Thr Ile Thr Cys Leu Ala Ser Gln Thr Ile Gly Thr Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Thr Pro Gly Lys Ser Pro Gln Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Thr Ser Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Lys Phe Ser Phe Lys Ile Ser Ser Leu Gln Ala 
65                  70                  75                  80  


Glu Asp Phe Ala Ser Tyr Tyr Cys Gln Gln Val Ser Ser Ile Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Arg 
            100                 105         


<210>  35
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huD2A4-V1 - huD2A4-V3 and huD2A4-V5

<400>  35

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Leu Ala Ser Gln Thr Ile Gly Thr Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ser Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Thr Ser Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Val Ser Ser Ile Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  36
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL for huD2A4-V4 and huD2A4-V6

<400>  36

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Leu Ala Ser Gln Thr Ile Gly Thr Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ala Ala Thr Ser Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Val Ser Ser Ile Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 for mouse, chimeric and humanized D2H3, defined by 
       Chothia numbering scheme

<400>  37

Gly Tyr Thr Phe Thr Asn Tyr 
1               5           


<210>  38
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 for mouse, chimeric and humanized D2H3, defined by Kabat 
       numbering scheme

<400>  38

Asn Tyr Trp Met His 
1               5   


<210>  39
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 for mouse, chimeric and humanized D2H3, defined by 
       Chothia numbering scheme

<400>  39

Phe Pro Arg Asn Ser Glu 
1               5       


<210>  40
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 for mouse, chimeric and humanized D2H3, defined by Kabat 
       numbering scheme

<400>  40

Ala Ile Phe Pro Arg Asn Ser Glu Thr Asn Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Ala 
    


<210>  41
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 for mouse, chimeric and humanized D2H3, defined by 
       Chothia or Kabat numbering scheme

<400>  41

Asn Arg Tyr Gly Leu Asp Tyr 
1               5           


<210>  42
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 for mouse, chimeric and humanized D2H3,  defined by IMGT 
       numbering scheme

<400>  42

Glu Ser Val Ser Leu His Gly Thr Arg Leu 
1               5                   10  


<210>  43
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR2 for mouse, chimeric and humanized D2H3,  defined by IMGT 
       numbering scheme

<400>  43

Leu Gly Ser 
1               


<210>  44
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 for mouse, chimeric and humanized D2A4, defined by 
       Chothia numbering scheme

<400>  44

Gly Phe Thr Phe Ser Ser Tyr 
1               5           


<210>  45
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR1 for mouse, chimeric and humanized D2A4, defined by Kabat 
       numbering scheme

<400>  45

Ser Tyr Thr Met Ser 
1               5   


<210>  46
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 for mouse, chimeric and humanized D2A4,  defined by 
       Chothia numbering scheme

<400>  46

Ser Gly Gly Gly Ser Asn 
1               5       


<210>  47
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR2 for mouse, chimeric and humanized D2A4, defined by Kabat 
       numbering scheme

<400>  47

Thr Ile Ser Gly Gly Gly Ser Asn Thr Tyr Tyr Pro Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  48
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH-CDR3 for mouse, chimeric and humanized D2A4, defined by 
       Chothia or Kabat numbering scheme

<400>  48

Gln Ala Phe Tyr Ser Asn Tyr Trp Tyr Phe Asp Val 
1               5                   10          


<210>  49
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CDR1 for mouse, chimeric and humanized D2A4, defined by IMGT 
       numbering scheme

<400>  49

Gln Thr Ile Gly Thr Trp 
1               5       


<210>  50
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL -CDR2 for mouse, chimeric and humanized D2A4, defined by IMGT 
       numbering scheme

<400>  50

Ala Ala Thr 
1               


<210>  51
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1 heavy chain constant region

<400>  51

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  52
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human kappa light chain constant region

<400>  52

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  53
<211>  371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human PD-1-Fc

<400>  53

Leu Asp Ser Pro Asp Arg Pro Trp Asn Pro Pro Thr Phe Ser Pro Ala 
1               5                   10                  15      


Leu Leu Val Val Thr Glu Gly Asp Asn Ala Thr Phe Thr Cys Ser Phe 
            20                  25                  30          


Ser Asn Thr Ser Glu Ser Phe Val Leu Asn Trp Tyr Arg Met Ser Pro 
        35                  40                  45              


Ser Asn Gln Thr Asp Lys Leu Ala Ala Phe Pro Glu Asp Arg Ser Gln 
    50                  55                  60                  


Pro Gly Gln Asp Cys Arg Phe Arg Val Thr Gln Leu Pro Asn Gly Arg 
65                  70                  75                  80  


Asp Phe His Met Ser Val Val Arg Ala Arg Arg Asn Asp Ser Gly Thr 
                85                  90                  95      


Tyr Leu Cys Gly Ala Ile Ser Leu Ala Pro Lys Ala Gln Ile Lys Glu 
            100                 105                 110         


Ser Leu Arg Ala Glu Leu Arg Val Thr Glu Arg Arg Ala Glu Val Pro 
        115                 120                 125             


Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln Phe Gln Glu 
    130                 135                 140                 


Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
145                 150                 155                 160 


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
        195                 200                 205             


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
225                 230                 235                 240 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
                245                 250                 255     


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
            260                 265                 270         


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 
        275                 280                 285             


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
    290                 295                 300                 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
305                 310                 315                 320 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
                325                 330                 335     


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
            340                 345                 350         


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
        355                 360                 365             


Leu Ser Leu 
    370     


<210>  54
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human PD-L1-Fc

<400>  54

Phe Thr Val Thr Val Pro Lys Asp Leu Tyr Val Val Glu Tyr Gly Ser 
1               5                   10                  15      


Asn Met Thr Ile Glu Cys Lys Phe Pro Val Glu Lys Gln Leu Asp Leu 
            20                  25                  30          


Ala Ala Leu Ile Val Tyr Trp Glu Met Glu Asp Lys Asn Ile Ile Gln 
        35                  40                  45              


Phe Val His Gly Glu Glu Asp Leu Lys Val Gln His Ser Ser Tyr Arg 
    50                  55                  60                  


Gln Arg Ala Arg Leu Leu Lys Asp Gln Leu Ser Leu Gly Asn Ala Ala 
65                  70                  75                  80  


Leu Gln Ile Thr Asp Val Lys Leu Gln Asp Ala Gly Val Tyr Arg Cys 
                85                  90                  95      


Met Ile Ser Tyr Gly Gly Ala Asp Tyr Lys Arg Ile Thr Val Lys Val 
            100                 105                 110         


Asn Ala Pro Tyr Asn Lys Ile Asn Gln Arg Ile Leu Val Val Asp Pro 
        115                 120                 125             


Val Thr Ser Glu His Glu Leu Thr Cys Gln Ala Glu Gly Tyr Pro Lys 
    130                 135                 140                 


Ala Glu Val Ile Trp Thr Ser Ser Asp His Gln Val Leu Ser Gly Lys 
145                 150                 155                 160 


Thr Thr Thr Thr Asn Ser Lys Arg Glu Glu Lys Leu Phe Asn Val Thr 
                165                 170                 175     


Ser Thr Leu Arg Ile Asn Thr Thr Thr Asn Glu Ile Phe Tyr Cys Thr 
            180                 185                 190         


Phe Arg Arg Leu Asp Pro Glu Glu Asn His Thr Ala Glu Leu Val Ile 
        195                 200                 205             


Pro Glu Leu Pro Leu Ala His Pro Pro Asn Glu Arg Thr Glu Pro Lys 
    210                 215                 220                 


Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 
225                 230                 235                 240 


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
                245                 250                 255     


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
            260                 265                 270         


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
        275                 280                 285             


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
    290                 295                 300                 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
305                 310                 315                 320 


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
                325                 330                 335     


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
            340                 345                 350         


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
        355                 360                 365             


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
    370                 375                 380                 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
385                 390                 395                 400 


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
                405                 410                 415     


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
            420                 425                 430         


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
        435                 440                 445             


Leu Ser Pro Gly Lys 
    450             


<210>  55
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH for mouse D2H3

<400>  55
gaggttcagc tccagcagtc tgggactgtg ctggcaaggc ctggggcctc agtgaagatg       60

tcctgcaagt cttctggcta cacctttacc aactactgga tgcactgggt aaaacagagg      120

cctggacagg gtctggaatg gattggcgct atttttccta gaaatagtga gactaactac      180

aaccagaaat ttaaggccaa ggccaaactg actgcagtca catctgccag cactgcctac      240

atggaggtca gcagcctgac aagtgaggac tctgcggtct atttctgtac gaggaatagg      300

tatggtctgg actactgggg tcaaggaacc tcagtcaccg tctcctca                   348


<210>  56
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH for huD2H3-V12 and huD2H3-V14

<400>  56
gaggtgcagc tggtgcagag cggcgccgag gtgaagaagc ccggctccag cgtgaaggtg       60

agctgcaagg ccagcgggta caccttcaca aactactgga tgcactgggt gaggcaggcc      120

ccagggcagg gcctggagtg gatgggcgcc atcttcccca ggaacagcga gacaaactac      180

aaccagaagt tcaaggccag ggtgacaatc acagccgatg agagcaccag cacagcctac      240

atggagctga gcagcctgcg gagcgaggac accgccgtgt actactgcac ccggaaccgg      300

tacgggctgg attactgggg gcaggggacc ctggtgacag tgagcagc                   348


<210>  57
<211>  363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH for mouse D2A4

<400>  57
gaagtgaagc tggtggagtc tgggggaggc ttagtgaagc ctggagggtc cctgaaactc       60

tcctgtgcag cctctggatt cactttcagt agctatacca tgtcttgggt tcgccagact      120

ccggcgaaga ggctggagtg ggtcgcaacc attagtggag gtggtagtaa cacctactat      180

cctgacagtg tgaagggccg attcaccatc tccagagaca atgccaggaa caccctgtac      240

ctgcaaatga gcagtctgag gtctgaggac acggccatgt attactgtgc aagacaagcc      300

ttctatagta actactggta cttcgacgtc tggggcgcag ggaccacggt caccgtctcc      360

tca                                                                    363


<210>  58
<211>  363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH for huD2A4-V5 and huD2A4-V6

<400>  58
gaggtgcagc tggtggagag cgggggggga ctggtgcagc caggaggaag cctgagactg       60

agctgtgccg caagcgggtt cacatttagt agttacacaa tgagctgggt gagacaggcc      120

cccggaaaag gactggagtg ggtgtctact atttcaggag ggggaagcaa cacctattat      180

cccgatagtg tgaagggcag attcacaatc agtagagaca acagcaagaa caccctgtac      240

ctgcagatga acagcctgag agccgaggac accgccgtgt actactgcgc cagacaggcc      300

ttttacagta actattggta cttcgacgtg tggggaaagg gaaccacagt gactgtgagc      360

agc                                                                    363


<210>  59
<211>  333
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL for mouse D2H3

<400>  59
gacatcgtgc tgacccaatc tccaggtttt ttggctgtgt ctctagggca gagggccacc       60

atctcctgca gagccagtga aagtgtcagt cttcatggta ctcgtttaat gcactggtac      120

catcagaaac caggacagcc acccaaactc ctcatctctc ttggatccaa cctagagtct      180

ggagtccctg ccaggttcag tggcagtggg tctgagacag acttcaccct caacatccat      240

cctgtggagg aggaggatgc tgcaacctat ttctgtcagc aaagtattga ggatccgtgg      300

acgttcggtg gaggcaccaa gctggaaatc aaa                                   333


<210>  60
<211>  333
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL for huD2H3-V13 and huD2H3-V14

<400>  60
gatatcgtga tgacacagag ccccgatagc ctggccgtga gcctggggga gcgggccacc       60

atcaactgcc gggccagcga gagcgtgagc ctgcacggga caaggctgat gcactggtac      120

cagcagaagc ccggccagcc ccccaagctg ctgatctacc tggggagcaa cctggagagc      180

ggggtgcccg ataggttcag cggcagcggg agcggcacag atttcacact gaccatcagc      240

agcctgcagg ccgaggacgt ggccgtgtac tactgccagc agagcatcga ggacccctgg      300

accttcggcc agggcacaaa gctggagatc aag                                   333


<210>  61
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL for mouse D2A4

<400>  61
gacattcaga tgacccagtc tcctgcctcc cagtctgcat ctctgggaga aagtgtcacc       60

atcacatgcc tggcaagtca gaccattggt acatggttag catggtatca gcagacacca      120

gggaaatctc ctcagctcct gatttatgct gcaaccagct tggcagatgg ggtcccatca      180

aggttcagtg gtagtggatc tggcacaaag ttttctttca agatcagcag cctacaggct      240

gaagattttg caagttatta ctgtcaacaa gtttccagta ttccgtggac gttcggtgga      300

ggcaccaagc tggaaatcag a                                                321


<210>  62
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL for huD2A4-V4 and huD2A4-V6

<400>  62
gacattcaga tgacccagag ccccagcagc gtgagcgcca gcgtgggaga cagagtgacc       60

ataacctgcc tggccagcca aaccataggc acctggctgg cctggtacca gcagaaaccc      120

ggcaaagccc ccaaactgct catctacgcc gccaccagcc tggctgacgg agtgccaagc      180

agattctccg gtagcggcag cggcaccgac ttcaccctga ctatcagcag cctccaaccc      240

gaagacttcg ccacctacta ctgccaacag gtctcctcca ttccctggac cttcggaggc      300

ggcaccaagg tggagatcaa a                                                321


<210>  63
<211>  990
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human IgG1 heavy chain constant region

<400>  63
gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg       60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca      180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc      300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga      360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct      420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg      480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac      540

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag      600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc      660

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag      720

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc      780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg      840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg      900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg      960

cagaagagcc tctccctgtc tccgggtaaa                                       990


<210>  64
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human kappa light chain constant region

<400>  64
cgtacggtgg cggcgccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct       60

ggaactgcct ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag      120

tggaaggtgg ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac      180

agcaaggaca gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag      240

aaacacaaag tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag      300

agcttcaaca ggggagagtg t                                                321


<210>  65
<211>  324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse IgG1 heavy chain constant region

<400>  65

Ala Lys Thr Thr Pro Pro Ser Val Tyr Pro Leu Ala Pro Gly Ser Ala 
1               5                   10                  15      


Ala Gln Thr Asn Ser Met Val Thr Leu Gly Cys Leu Val Lys Gly Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ser Leu Ser Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Asp Leu Tyr Thr Leu 
    50                  55                  60                  


Ser Ser Ser Val Thr Val Pro Ser Ser Thr Trp Pro Ser Glu Thr Val 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys 
                85                  90                  95      


Ile Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys Thr Val Pro 
            100                 105                 110         


Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Val Leu 
        115                 120                 125             


Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Ile Ser 
    130                 135                 140                 


Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp Trp Leu Asn 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe Pro Ala Pro 
        195                 200                 205             


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys Ala Pro Gln 
    210                 215                 220                 


Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp Lys Val 
225                 230                 235                 240 


Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile Thr Val 
                245                 250                 255     


Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn Thr Gln 
            260                 265                 270         


Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys Leu Asn 
        275                 280                 285             


Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys Ser Val 
    290                 295                 300                 


Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu Ser His 
305                 310                 315                 320 


Ser Pro Gly Lys 
                


<210>  66
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse kappa light chain constant region

<400>  66

Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Ser Glu 
1               5                   10                  15      


Gln Leu Thr Ser Gly Gly Ala Ser Val Val Cys Phe Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Lys Asp Ile Asn Val Lys Trp Lys Ile Asp Gly Ser Glu Arg 
        35                  40                  45              


Gln Asn Gly Val Leu Asn Ser Trp Thr Asp Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Met Ser Ser Thr Leu Thr Leu Thr Lys Asp Glu Tyr Glu 
65                  70                  75                  80  


Arg His Asn Ser Tyr Thr Cys Glu Ala Thr His Lys Thr Ser Thr Ser 
                85                  90                  95      


Pro Ile Val Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  67
<211>  972
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse IgG1 heavy chain constant regio

<400>  67
gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac       60

tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc      120

tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac      180

ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc      240

acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg      300

gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc      360

cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg      420

gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag      480

gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc      540

agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc      600

aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg      660

aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc      720

agtctgacct gcatgataac agacttcttc cctgaagaca ttactgtgga gtggcagtgg      780

aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct      840

tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc      900

acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac      960

tctcctggta aa                                                          972


<210>  68
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse kappa light chain constant region

<400>  68
cgggctgatg ctgcaccaac tgtatccatc ttcccaccat ccagtgagca gttaacatct       60

ggaggtgcct cagtcgtgtg cttcttgaac aacttctacc ccaaagacat caatgtcaag      120

tggaagattg atggcagtga acgacaaaat ggcgtcctga acagttggac tgatcaggac      180

agcaaagaca gcacctacag catgagcagc accctcacgt tgactaagga cgagtatgaa      240

cgacataaca gctatacctg tgaggccact cacaagacat caacttcacc cattgtcaag      300

agcttcaaca ggggagagtg t                                                321

