                         SEQUENCE LISTING

<110>  йѧԺо
 
<120>  һIFN뿹PD-L1ںϵ׼Ӧ

<130>  PCT190215

<160>  48    

<170>  PatentIn version 3.3

<210>  1
<211>  472
<212>  PRT
<213>  ԴAnti-PD-L1ScFvPD-L1-Fc-knobİ

<400>  1

Glu Leu Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser 
1               5                   10                  15      


Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser 
            20                  25                  30          


Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu 
65                  70                  75                  80  


Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His 
                85                  90                  95      


Pro Ala Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly 
            100                 105                 110         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln 
        115                 120                 125             


Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg 
    130                 135                 140                 


Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp Ile His 
145                 150                 155                 160 


Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Trp Ile 
                165                 170                 175     


Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg 
            180                 185                 190         


Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met 
        195                 200                 205             


Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg 
    210                 215                 220                 


His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ala Arg Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
                245                 250                 255     


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            260                 265                 270         


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        275                 280                 285             


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    290                 295                 300                 


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
305                 310                 315                 320 


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                325                 330                 335     


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            340                 345                 350         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        355                 360                 365             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Cys Arg Asp Glu Leu Thr 
    370                 375                 380                 


Lys Asn Gln Val Ser Leu Trp Cys Leu Val Lys Gly Phe Tyr Pro Ser 
385                 390                 395                 400 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                405                 410                 415     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            420                 425                 430         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        435                 440                 445             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    450                 455                 460                 


Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470         


<210>  2
<211>  413
<212>  PRT
<213>  ԴmIFNa4-Fc-holeİ

<400>  2

Cys Asp Leu Pro His Thr Tyr Asn Leu Gly Asn Lys Arg Ala Leu Thr 
1               5                   10                  15      


Val Leu Glu Glu Met Arg Arg Leu Pro Pro Leu Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys Val Asp Asn Gln Gln Ile 
        35                  40                  45              


Gln Lys Ala Gln Ala Ile Leu Val Leu Arg Asp Leu Thr Gln Gln Ile 
    50                  55                  60                  


Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser Ala Thr Trp Asn Ala Thr 
65                  70                  75                  80  


Leu Leu Asp Ser Phe Cys Asn Asp Leu His Gln Gln Leu Asn Asp Leu 
                85                  90                  95      


Lys Ala Cys Val Met Gln Glu Pro Pro Leu Thr Gln Glu Asp Ser Leu 
            100                 105                 110         


Leu Ala Val Arg Thr Tyr Phe His Arg Ile Thr Val Tyr Leu Arg Lys 
        115                 120                 125             


Lys Lys His Ser Leu Cys Ala Trp Glu Val Ile Arg Ala Glu Val Trp 
    130                 135                 140                 


Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu Ala Arg Leu Ser Glu Glu 
145                 150                 155                 160 


Lys Glu Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Gly Ser Gly Gly Gly Gly Arg Thr Asp Lys Thr His Thr Cys Pro Pro 
            180                 185                 190         


Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 
        195                 200                 205             


Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 
    210                 215                 220                 


Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 
225                 230                 235                 240 


Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 
                245                 250                 255     


Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 
            260                 265                 270         


Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 
        275                 280                 285             


Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
    290                 295                 300                 


Gly Gln Pro Arg Glu Pro Gln Val Cys Thr Leu Pro Pro Ser Arg Asp 
305                 310                 315                 320 


Glu Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala Val Lys Gly Phe 
                325                 330                 335     


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
            340                 345                 350         


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
        355                 360                 365             


Phe Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
    370                 375                 380                 


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
385                 390                 395                 400 


Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys His Val 
                405                 410             


<210>  3
<211>  660
<212>  PRT
<213>  ͬԴmIFNa4-anti-PD-L1-Fcİ

<400>  3

Cys Asp Leu Pro His Thr Tyr Asn Leu Gly Asn Lys Arg Ala Leu Thr 
1               5                   10                  15      


Val Leu Glu Glu Met Arg Arg Leu Pro Pro Leu Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys Val Asp Asn Gln Gln Ile 
        35                  40                  45              


Gln Lys Ala Gln Ala Ile Leu Val Leu Arg Asp Leu Thr Gln Gln Ile 
    50                  55                  60                  


Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser Ala Thr Trp Asn Ala Thr 
65                  70                  75                  80  


Leu Leu Asp Ser Phe Cys Asn Asp Leu His Gln Gln Leu Asn Asp Leu 
                85                  90                  95      


Lys Ala Cys Val Met Gln Glu Pro Pro Leu Thr Gln Glu Asp Ser Leu 
            100                 105                 110         


Leu Ala Val Arg Thr Tyr Phe His Arg Ile Thr Val Tyr Leu Arg Lys 
        115                 120                 125             


Lys Lys His Ser Leu Cys Ala Trp Glu Val Ile Arg Ala Glu Val Trp 
    130                 135                 140                 


Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu Ala Arg Leu Ser Glu Glu 
145                 150                 155                 160 


Lys Glu Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Gly Ser Gly Gly Gly Gly Arg Thr Asp Ile Gln Met Thr Gln Ser Pro 
            180                 185                 190         


Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg 
        195                 200                 205             


Ala Ser Gln Asp Val Ser Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro 
    210                 215                 220                 


Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser 
225                 230                 235                 240 


Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr 
                245                 250                 255     


Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys 
            260                 265                 270         


Gln Gln Tyr Leu Tyr His Pro Ala Thr Phe Gly Gln Gly Thr Lys Val 
        275                 280                 285             


Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    290                 295                 300                 


Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
305                 310                 315                 320 


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
                325                 330                 335     


Ser Asp Ser Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
            340                 345                 350         


Glu Trp Val Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala 
        355                 360                 365             


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn 
    370                 375                 380                 


Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
385                 390                 395                 400 


Tyr Tyr Cys Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly 
                405                 410                 415     


Gln Gly Thr Leu Val Thr Val Ser Ala Phe Glu Glu Pro Lys Ser Cys 
            420                 425                 430         


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
        435                 440                 445             


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Gln Leu Met 
    450                 455                 460                 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
465                 470                 475                 480 


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
                485                 490                 495     


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
            500                 505                 510         


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
        515                 520                 525             


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
    530                 535                 540                 


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
545                 550                 555                 560 


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
                565                 570                 575     


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
            580                 585                 590         


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
        595                 600                 605             


Val Leu Asp Ser Asp Gly Ser Phe Leu Tyr Ser Lys Leu Thr Val Asp 
    610                 615                 620                 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Leu His 
625                 630                 635                 640 


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
                645                 650                 655     


Gly Lys His Val 
            660 


<210>  4
<211>  1482
<212>  DNA
<213>  Anti-PD-L1-Fc-knob(ԴAnti-PD-L1ı)

<400>  4
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt     60

gacgagctcg acatccagat gacccagagc cccagcagcc tgagcgccag cgtgggcgac    120

agagtgacca tcacctgcag agccagccag gacgtgagca ccgccgtggc ctggtaccag    180

cagaagcccg gcaaggcccc caagctgctg atctacagcg ccagcttcct gtacagcggc    240

gtgcccagca gattcagcgg cagcggcagc ggcaccgact tcaccctgac catcagcagc    300

ctgcagcccg aggacttcgc cacctactac tgccagcagt acctgtacca ccccgccacc    360

ttcggccagg gcaccaaggt ggagatcaag agaggcggcg gcggcagcgg cggcggcggc    420

agcggcggcg gcggcagcga ggtgcagctg gtggagagcg gcggcggcct ggtgcagccc    480

ggcggcagcc tgagactgag ctgcgccgcc agcggcttca ccttcagcga cagctggatc    540

cactgggtga gacaggcccc cggcaagggc ctggagtggg tggcctggat cagcccctac    600

ggcggcagca cctactacgc cgacagcgtg aagggcagat tcaccatcag cgccgacacc    660

agcaagaaca ccgcctacct gcagatgaac agcctgagag ccgaggacac cgccgtgtac    720

tactgcgcca gaagacactg gcccggcggc ttcgactact ggggccaggg caccctggtg    780

accgtgagcg ccagatctga caagacccac acctgccccc cctgccccgc ccccgagctg    840

ctgggcggcc ccagcgtgtt cctgttcccc cccaagccca aggacaccct gatgatcagc    900

cgcacccccg aggtgacctg cgtggtggtg gacgtgagcc acgaggaccc cgaggtgaag    960

ttcaactggt acgtggacgg cgtggaggtg cacaacgcca agaccaagcc ccgcgaggag   1020

cagtacaaca gcacctaccg cgtggtgagc gtgctgaccg tgctgcacca ggactggctg   1080

aacggcaagg agtacaagtg caaggtgagc aacaaggccc tgcccgcccc catcgagaag   1140

accatcagca aggccaaggg ccagccccgc gagccccagg tgtacaccct gcccccctgc   1200

cgcgacgagc tgaccaagaa ccaggtgagc ctgtggtgcc tggtgaaggg cttctacccc   1260

agcgacatcg ccgtggagtg ggagagcaac ggccagcccg agaacaacta caagaccacc   1320

ccccccgtgc tggacagcga cggcagcttc ttcctgtaca gcaagctgac cgtggacaag   1380

agccgctggc agcagggcaa cgtgttcagc tgcagcgtga tgcacgaggc cctgcacaac   1440

cactacaccc agaagagcct gagcctgagc cccggcaagt aa                      1482


<210>  5
<211>  1311
<212>  DNA
<213>  mIFNa4-Fc-hole(ԴmIFNa4-Fcı)

<400>  5
gccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt     60

tccactggtt cgcgatgtga cctgcctcac acttataacc tcgggaacaa gagggccttg    120

acagtcctgg aagaaatgag aagactcccc cctctttcct gcctgaagga caggaaggat    180

tttggattcc ccttggagaa ggtggataac caacagatcc agaaggctca agccatcctt    240

gtgctaagag atcttaccca gcagattttg aacctcttca catcaaaaga cttgtctgct    300

acttggaatg caactctact agactcattc tgcaatgacc tccatcagca gctcaatgac    360

ctcaaagcct gtgtgatgca ggaacctcct ctgacccagg aagactccct gctggctgtg    420

aggacatact tccacaggat cactgtgtac ctgagaaaga agaaacacag cctctgtgcc    480

tgggaggtga tcagagcaga agtctggaga gccctctctt cctcaaccaa cttgctggca    540

agactgagtg aggagaagga gagtggcggt ggtggctccg gcggtggtgg ctccggcggt    600

ggtggctccg gcggtggtgg ccgtacggac aagacccaca cctgcccccc ctgccccgcc    660

cccgagctgc tgggcggccc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg    720

atgatcagcc gcacccccga ggtgacctgc gtggtggtgg acgtgagcca cgaggacccc    780

gaggtgaagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa gaccaagccc    840

cgcgaggagc agtacaacag cacctaccgc gtggtgagcg tgctgaccgt gctgcaccag    900

gactggctga acggcaagga gtacaagtgc aaggtgagca acaaggccct gcccgccccc    960

atcgagaaga ccatcagcaa ggccaagggc cagccccgcg agccccaggt gtgcaccctg   1020

ccccccagcc gcgacgagct gaccaagaac caggtgagcc tgagctgcgc cgtgaagggc   1080

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga gaacaactac   1140

aagaccaccc cccccgtgct ggacagcgac ggcagcttct tcctggtgag caagctgacc   1200

gtggacaaga gccgctggca gcagggcaac gtgttcagct gcagcgtgat gcacgaggcc   1260

ctgcacaacc actacaccca gaagagcctg agcctgagcc ccggcaagta a            1311


<210>  6
<211>  2052
<212>  DNA
<213>  mIFNa4-anti-PD-L1-Fc(ͬԴmIFNa4-anti-PD-L1-Fcı)

<400>  6
atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt     60

tcgcgatgtg acctgcctca cacttataac ctcgggaaca agagggcctt gacagtcctg    120

gaagaaatga gaagactccc ccctctttcc tgcctgaagg acaggaagga ttttggattc    180

cccttggaga aggtggataa ccaacagatc cagaaggctc aagccatcct tgtgctaaga    240

gatcttaccc agcagatttt gaacctcttc acatcaaaag acttgtctgc tacttggaat    300

gcaactctac tagactcatt ctgcaatgac ctccatcagc agctcaatga cctcaaagcc    360

tgtgtgatgc aggaacctcc tctgacccag gaagactccc tgctggctgt gaggacatac    420

ttccacagga tcactgtgta cctgagaaag aagaaacaca gcctctgtgc ctgggaggtg    480

atcagagcag aagtctggag agccctctct tcctcaacca acttgctggc aagactgagt    540

gaggagaagg agagtggcgg tggtggctcc ggcggtggtg gctccggcgg tggtggctcc    600

ggcggtggtg gccgtacgga catccagatg acccagagcc ccagcagcct gagcgccagc    660

gtgggcgaca gagtgaccat cacctgcaga gccagccagg acgtgagcac cgccgtggcc    720

tggtaccagc agaagcccgg caaggccccc aagctgctga tctacagcgc cagcttcctg    780

tacagcggcg tgcccagcag attcagcggc agcggcagcg gcaccgactt caccctgacc    840

atcagcagcc tgcagcccga ggacttcgcc acctactact gccagcagta cctgtaccac    900

cccgccacct tcggccaggg caccaaggtg gagatcaaga gaggcggcgg cggcagcggc    960

ggcggcggca gcggcggcgg cggcagcgag gtgcagctgg tggagagcgg cggcggcctg   1020

gtgcagcccg gcggcagcct gagactgagc tgcgccgcca gcggcttcac cttcagcgac   1080

agctggatcc actgggtgag acaggccccc ggcaagggcc tggagtgggt ggcctggatc   1140

agcccctacg gcggcagcac ctactacgcc gacagcgtga agggcagatt caccatcagc   1200

gccgacacca gcaagaacac cgcctacctg cagatgaaca gcctgagagc cgaggacacc   1260

gccgtgtact actgcgccag aagacactgg cccggcggct tcgactactg gggccagggc   1320

accctggtga ccgtgagcgc cttcgaagag cccaagagct gcgacaagac ccacacctgt   1380

cccccttgtc ctgcccctga gctgctgggc ggacccagcg tgttcctgtt ccccccaaag   1440

cccaaggacc agctgatgat cagccggacc cccgaagtga cctgcgtggt ggtggacgtg   1500

tcccacgagg accctgaagt gaagttcaat tggtacgtgg acggcgtgga ggtgcacaac   1560

gccaagacca agccccggga ggaacagtac aacagcacct accgggtggt gtccgtgctg   1620

accgtgctgc accaggactg gctgaacggc aaagagtaca agtgcaaggt ctccaacaag   1680

gccctgcctg cccccatcga aaagaccatc agcaaggcca agggccagcc cagagaaccc   1740

caggtgtaca ccctgccccc cagcagagat gagctgacca agaaccaggt gtccctgacc   1800

tgcctggtca agggcttcta ccccagcgat atcgccgtgg agtgggagag caacggccag   1860

cctgagaaca actacaagac caccccccct gtgctggaca gcgatggcag cttcctctac   1920

agcaaactga ccgtggacaa gagccggtgg cagcagggca acgtgttcag ctgcagcgtg   1980

ttgcacgagg ccctgcacaa ccactacacc cagaagtccc tgagcctgag ccccggcaaa   2040

cacgtgtagt aa                                                       2052


<210>  7
<211>  1314
<212>  DNA
<213>  mIFNb-Fc-hole(ԴmIFNb-Fcı)

<400>  7
aagcttgccg ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt     60

ccaggttcca ctggttcgcg aatcaactat aagcagctcc agctccaaga aaggacgaac    120

attcggaaat gtcaggagct cctggagcag ctgaatggaa agatcaacct cacctacagg    180

gcggacttca agatccctat ggagatgacg gagaagatgc agaagagtta cactgccttt    240

gccatccaag agatgctcca gaatgtcttt cttgtcttca gaaacaattt ctccagcact    300

gggtggaatg agactattgt tgtacgtctc ctggatgaac tccaccagca gacagtgttt    360

ctgaagacag tactagagga aaagcaagag gaaagattga cgtgggagat gtcctcaact    420

gctctccact tgaagagcta ttactggagg gtgcaaaggt accttaaact catgaagtac    480

aacagctacg cctggatggt ggtccgagca gagatcttca ggaactttct catcattcga    540

agacttacca gaaacttcca aaacagtggc ggtggtggct ccggcggtgg tggctccggc    600

ggtggtggct ccggcggtgg tggccgtacg gacaagaccc acacctgccc cccctgcccc    660

gcccccgagc tgctgggcgg ccccagcgtg ttcctgttcc cccccaagcc caaggacacc    720

ctgatgatca gccgcacccc cgaggtgacc tgcgtggtgg tggacgtgag ccacgaggac    780

cccgaggtga agttcaactg gtacgtggac ggcgtggagg tgcacaacgc caagaccaag    840

ccccgcgagg agcagtacaa cagcacctac cgcgtggtga gcgtgctgac cgtgctgcac    900

caggactggc tgaacggcaa ggagtacaag tgcaaggtga gcaacaaggc cctgcccgcc    960

cccatcgaga agaccatcag caaggccaag ggccagcccc gcgagcccca ggtgtgcacc   1020

ctgcccccca gccgcgacga gctgaccaag aaccaggtga gcctgagctg cgccgtgaag   1080

ggcttctacc ccagcgacat cgccgtggag tgggagagca acggccagcc cgagaacaac   1140

tacaagacca ccccccccgt gctggacagc gacggcagct tcttcctggt gagcaagctg   1200

accgtggaca agagccgctg gcagcagggc aacgtgttca gctgcagcgt gatgcacgag   1260

gccctgcaca accactacac ccagaagagc ctgagcctga gccccggcaa gtaa         1314


<210>  8
<211>  1326
<212>  DNA
<213>  hIFNa2-Fc-hole(ԴhIFN a2-Fcı)

<400>  8
aagcttgccg ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt     60

ccaggttcca ctggttcgcg atgtgatctg cctcaaaccc acagcctggg tagcaggagg    120

accttgatgc tcctggcaca gatgaggaga atctctcttt tctcctgctt gaaggacaga    180

catgactttg gatttcccca ggaggagttt ggcaaccagt tccaaaaggc tgaaaccatc    240

cctgtcctcc atgagatgat ccagcagatc ttcaatctct tcagcacaaa ggactcatct    300

gctgcttggg atgagaccct cctagacaaa ttctacactg aactctacca gcagctgaat    360

gacctggaag cctgtgtgat acagggggtg ggggtgacag agactcccct gatgaaggag    420

gactccattc tggctgtgag gaaatacttc caaagaatca ctctctatct gaaagagaag    480

aaatacagcc cttgtgcctg ggaggttgtc agagcagaaa tcatgagatc tttttctttg    540

tcaacaaact tgcaagaaag tttaagaagt aaggaaagtg gcggtggtgg ctccggcggt    600

ggtggctccg gcggtggtgg ctccggcggt ggtggccgta cggacaagac ccacacctgc    660

cccccctgcc ccgcccccga gctgctgggc ggccccagcg tgttcctgtt cccccccaag    720

cccaaggaca ccctgatgat cagccgcacc cccgaggtga cctgcgtggt ggtggacgtg    780

agccacgagg accccgaggt gaagttcaac tggtacgtgg acggcgtgga ggtgcacaac    840

gccaagacca agccccgcga ggagcagtac aacagcacct accgcgtggt gagcgtgctg    900

accgtgctgc accaggactg gctgaacggc aaggagtaca agtgcaaggt gagcaacaag    960

gccctgcccg cccccatcga gaagaccatc agcaaggcca agggccagcc ccgcgagccc   1020

caggtgtgca ccctgccccc cagccgcgac gagctgacca agaaccaggt gagcctgagc   1080

tgcgccgtga agggcttcta ccccagcgac atcgccgtgg agtgggagag caacggccag   1140

cccgagaaca actacaagac cacccccccc gtgctggaca gcgacggcag cttcttcctg   1200

gtgagcaagc tgaccgtgga caagagccgc tggcagcagg gcaacgtgtt cagctgcagc   1260

gtgatgcacg aggccctgca caaccactac acccagaaga gcctgagcct gagccccggc   1320

aagtaa                                                              1326


<210>  9
<211>  1329
<212>  DNA
<213>  hIFNb-Fc-hole(ԴhIFNb-Fcı)

<400>  9
aagcttgccg ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt     60

ccaggttcca ctggttcgcg aatgagctac aacttgcttg gattcctaca aagaagcagc    120

aattttcagt gtcagaagct cctgtggcaa ttgaatggga ggcttgaata ctgcctcaag    180

gacaggatga actttgacat ccctgaggag attaagcagc tgcagcagtt ccagaaggag    240

gacgccgcat tgaccatcta tgagatgctc cagaacatct ttgctatttt cagacaagat    300

tcatctagca ctggctggaa tgagactatt gttgagaacc tcctggctaa tgtctatcat    360

cagataaacc atctgaagac agtcctggaa gaaaaactgg agaaagaaga tttcaccagg    420

ggaaaactca tgagcagtct gcacctgaaa agatattatg ggaggattct gcattacctg    480

aaggccaagg agtacagtca ctgtgcctgg accatagtca gagtggaaat cctaaggaac    540

ttttacttca ttaacagact tacaggttac ctccgaaaca gtggcggtgg tggctccggc    600

ggtggtggct ccggcggtgg tggctccggc ggtggtggcc gtacggacaa gacccacacc    660

tgccccccct gccccgcccc cgagctgctg ggcggcccca gcgtgttcct gttccccccc    720

aagcccaagg acaccctgat gatcagccgc acccccgagg tgacctgcgt ggtggtggac    780

gtgagccacg aggaccccga ggtgaagttc aactggtacg tggacggcgt ggaggtgcac    840

aacgccaaga ccaagccccg cgaggagcag tacaacagca cctaccgcgt ggtgagcgtg    900

ctgaccgtgc tgcaccagga ctggctgaac ggcaaggagt acaagtgcaa ggtgagcaac    960

aaggccctgc ccgcccccat cgagaagacc atcagcaagg ccaagggcca gccccgcgag   1020

ccccaggtgt gcaccctgcc ccccagccgc gacgagctga ccaagaacca ggtgagcctg   1080

agctgcgccg tgaagggctt ctaccccagc gacatcgccg tggagtggga gagcaacggc   1140

cagcccgaga acaactacaa gaccaccccc cccgtgctgg acagcgacgg cagcttcttc   1200

ctggtgagca agctgaccgt ggacaagagc cgctggcagc agggcaacgt gttcagctgc   1260

agcgtgatgc acgaggccct gcacaaccac tacacccaga agagcctgag cctgagcccc   1320

ggcaagtaa                                                           1329


<210>  10
<211>  1224
<212>  DNA
<213>  mIFN-Fc-hole(ԴmIFN-Fcı)

<400>  10
aagcttgccg ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt     60

ccaggttcca ctggttcgcg acacggcaca gtcattgaaa gcctagaaag tctgaataac    120

tattttaact caagtggcat agatgtggaa gaaaagagtc tcttcttgga tatctggagg    180

aactggcaaa aggatggtga catgaaaatc ctgcagagcc agattatctc tttctacctc    240

agactctttg aagtcttgaa agacaatcag gccatcagca acaacataag cgtcattgaa    300

tcacacctga ttactacctt cttcagcaac agcaaggcga aaaaggatgc attcatgagt    360

attgccaagt ttgaggtcaa caacccacag gtccagcgcc aagcattcaa tgagctcatc    420

cgagtggtcc accagctgtt gccggaatcc agcctcagga agcggaaaag gagtcgctgc    480

agtggcggtg gtggctccgg cggtggtggc tccggcggtg gtggctccgg cggtggtggc    540

gacaagaccc acacctgccc cccctgcccc gcccccgagc tgctgggcgg ccccagcgtg    600

ttcctgttcc cccccaagcc caaggacacc ctgatgatca gccgcacccc cgaggtgacc    660

tgcgtggtgg tggacgtgag ccacgaggac cccgaggtga agttcaactg gtacgtggac    720

ggcgtggagg tgcacaacgc caagaccaag ccccgcgagg agcagtacaa cagcacctac    780

cgcgtggtga gcgtgctgac cgtgctgcac caggactggc tgaacggcaa ggagtacaag    840

tgcaaggtga gcaacaaggc cctgcccgcc cccatcgaga agaccatcag caaggccaag    900

ggccagcccc gcgagcccca ggtgtgcacc ctgcccccca gccgcgacga gctgaccaag    960

aaccaggtga gcctgagctg cgccgtgaag ggcttctacc ccagcgacat cgccgtggag   1020

tgggagagca acggccagcc cgagaacaac tacaagacca ccccccccgt gctggacagc   1080

gacggcagct tcttcctggt gagcaagctg accgtggaca agagccgctg gcagcagggc   1140

aacgtgttca gctgcagcgt gatgcacgag gccctgcaca accactacac ccagaagagc   1200

ctgagcctga gccccggcaa gtaa                                          1224


<210>  11
<211>  241
<212>  PRT
<213>  ScFvPD-L1

<400>  11

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Gly Gly Gly 
            100                 105                 110         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val 
        115                 120                 125             


Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser 
    130                 135                 140                 


Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser Trp Ile His Trp Val 
145                 150                 155                 160 


Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Trp Ile Ser Pro 
                165                 170                 175     


Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr 
            180                 185                 190         


Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn Ser 
        195                 200                 205             


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Arg His Trp 
    210                 215                 220                 


Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
225                 230                 235                 240 


Ala 
    


<210>  12
<211>  723
<212>  DNA
<213>  ScFvPD-L1

<400>  12
gacatccaga tgacccagag ccccagcagc ctgagcgcca gcgtgggcga cagagtgacc     60

atcacctgca gagccagcca ggacgtgagc accgccgtgg cctggtacca gcagaagccc    120

ggcaaggccc ccaagctgct gatctacagc gccagcttcc tgtacagcgg cgtgcccagc    180

agattcagcg gcagcggcag cggcaccgac ttcaccctga ccatcagcag cctgcagccc    240

gaggacttcg ccacctacta ctgccagcag tacctgtacc accccgccac cttcggccag    300

ggcaccaagg tggagatcaa gagaggcggc ggcggcagcg gcggcggcgg cagcggcggc    360

ggcggcagcg aggtgcagct ggtggagagc ggcggcggcc tggtgcagcc cggcggcagc    420

ctgagactga gctgcgccgc cagcggcttc accttcagcg acagctggat ccactgggtg    480

agacaggccc ccggcaaggg cctggagtgg gtggcctgga tcagccccta cggcggcagc    540

acctactacg ccgacagcgt gaagggcaga ttcaccatca gcgccgacac cagcaagaac    600

accgcctacc tgcagatgaa cagcctgaga gccgaggaca ccgccgtgta ctactgcgcc    660

agaagacact ggcccggcgg cttcgactac tggggccagg gcaccctggt gaccgtgagc    720

gcc                                                                  723


<210>  13
<211>  162
<212>  PRT
<213>  mIFN-4

<400>  13

Cys Asp Leu Pro His Thr Tyr Asn Leu Gly Asn Lys Arg Ala Leu Thr 
1               5                   10                  15      


Val Leu Glu Glu Met Arg Arg Leu Pro Pro Leu Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys Val Asp Asn Gln Gln Ile 
        35                  40                  45              


Gln Lys Ala Gln Ala Ile Leu Val Leu Arg Asp Leu Thr Gln Gln Ile 
    50                  55                  60                  


Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser Ala Thr Trp Asn Ala Thr 
65                  70                  75                  80  


Leu Leu Asp Ser Phe Cys Asn Asp Leu His Gln Gln Leu Asn Asp Leu 
                85                  90                  95      


Lys Ala Cys Val Met Gln Glu Pro Pro Leu Thr Gln Glu Asp Ser Leu 
            100                 105                 110         


Leu Ala Val Arg Thr Tyr Phe His Arg Ile Thr Val Tyr Leu Arg Lys 
        115                 120                 125             


Lys Lys His Ser Leu Cys Ala Trp Glu Val Ile Arg Ala Glu Val Trp 
    130                 135                 140                 


Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu Ala Arg Leu Ser Glu Glu 
145                 150                 155                 160 


Lys Glu 
        


<210>  14
<211>  486
<212>  DNA
<213>  mIFN-4

<400>  14
tgtgacctgc ctcacactta taacctcggg aacaagaggg ccttgacagt cctggaagaa     60

atgagaagac tcccccctct ttcctgcctg aaggacagga aggattttgg attccccttg    120

gagaaggtgg ataaccaaca gatccagaag gctcaagcca tccttgtgct aagagatctt    180

acccagcaga ttttgaacct cttcacatca aaagacttgt ctgctacttg gaatgcaact    240

ctactagact cattctgcaa tgacctccat cagcagctca atgacctcaa agcctgtgtg    300

atgcaggaac ctcctctgac ccaggaagac tccctgctgg ctgtgaggac atacttccac    360

aggatcactg tgtacctgag aaagaagaaa cacagcctct gtgcctggga ggtgatcaga    420

gcagaagtct ggagagccct ctcttcctca accaacttgc tggcaagact gagtgaggag    480

aaggag                                                               486


<210>  15
<211>  233
<212>  PRT
<213>  SEQ ID NO.15IgG1-Fc

<400>  15

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Gln Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Leu His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys His Val 
225                 230             


<210>  16
<211>  699
<212>  DNA
<213>  IgG1- Fc

<400>  16
gagcccaaga gctgcgacaa gacccacacc tgtccccctt gtcctgcccc tgagctgctg     60

ggcggaccca gcgtgttcct gttcccccca aagcccaagg accagctgat gatcagccgg    120

acccccgaag tgacctgcgt ggtggtggac gtgtcccacg aggaccctga agtgaagttc    180

aattggtacg tggacggcgt ggaggtgcac aacgccaaga ccaagccccg ggaggaacag    240

tacaacagca cctaccgggt ggtgtccgtg ctgaccgtgc tgcaccagga ctggctgaac    300

ggcaaagagt acaagtgcaa ggtctccaac aaggccctgc ctgcccccat cgaaaagacc    360

atcagcaagg ccaagggcca gcccagagaa ccccaggtgt acaccctgcc ccccagcaga    420

gatgagctga ccaagaacca ggtgtccctg acctgcctgg tcaagggctt ctaccccagc    480

gatatcgccg tggagtggga gagcaacggc cagcctgaga acaactacaa gaccaccccc    540

cctgtgctgg acagcgatgg cagcttcctc tacagcaaac tgaccgtgga caagagccgg    600

tggcagcagg gcaacgtgtt cagctgcagc gtgttgcacg aggccctgca caaccactac    660

acccagaagt ccctgagcct gagccccggc aaacacgtg                           699


<210>  17
<211>  161
<212>  PRT
<213>  mIFNb

<400>  17

Ile Asn Tyr Lys Gln Leu Gln Leu Gln Glu Arg Thr Asn Ile Arg Lys 
1               5                   10                  15      


Cys Gln Glu Leu Leu Glu Gln Leu Asn Gly Lys Ile Asn Leu Thr Tyr 
            20                  25                  30          


Arg Ala Asp Phe Lys Ile Pro Met Glu Met Thr Glu Lys Met Gln Lys 
        35                  40                  45              


Ser Tyr Thr Ala Phe Ala Ile Gln Glu Met Leu Gln Asn Val Phe Leu 
    50                  55                  60                  


Val Phe Arg Asn Asn Phe Ser Ser Thr Gly Trp Asn Glu Thr Ile Val 
65                  70                  75                  80  


Val Arg Leu Leu Asp Glu Leu His Gln Gln Thr Val Phe Leu Lys Thr 
                85                  90                  95      


Val Leu Glu Glu Lys Gln Glu Glu Arg Leu Thr Trp Glu Met Ser Ser 
            100                 105                 110         


Thr Ala Leu His Leu Lys Ser Tyr Tyr Trp Arg Val Gln Arg Tyr Leu 
        115                 120                 125             


Lys Leu Met Lys Tyr Asn Ser Tyr Ala Trp Met Val Val Arg Ala Glu 
    130                 135                 140                 


Ile Phe Arg Asn Phe Leu Ile Ile Arg Arg Leu Thr Arg Asn Phe Gln 
145                 150                 155                 160 


Asn 
    


<210>  18
<211>  483
<212>  DNA
<213>  mIFNb

<400>  18
atcaactata agcagctcca gctccaagaa aggacgaaca ttcggaaatg tcaggagctc     60

ctggagcagc tgaatggaaa gatcaacctc acctacaggg cggacttcaa gatccctatg    120

gagatgacgg agaagatgca gaagagttac actgcctttg ccatccaaga gatgctccag    180

aatgtctttc ttgtcttcag aaacaatttc tccagcactg ggtggaatga gactattgtt    240

gtacgtctcc tggatgaact ccaccagcag acagtgtttc tgaagacagt actagaggaa    300

aagcaagagg aaagattgac gtgggagatg tcctcaactg ctctccactt gaagagctat    360

tactggaggg tgcaaaggta ccttaaactc atgaagtaca acagctacgc ctggatggtg    420

gtccgagcag agatcttcag gaactttctc atcattcgaa gacttaccag aaacttccaa    480

aac                                                                  483


<210>  19
<211>  165
<212>  PRT
<213>  hIFNa2

<400>  19

Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met 
1               5                   10                  15      


Leu Leu Ala Gln Met Arg Arg Ile Ser Leu Phe Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln 
        35                  40                  45              


Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe 
    50                  55                  60                  


Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu 
65                  70                  75                  80  


Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu 
                85                  90                  95      


Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys 
            100                 105                 110         


Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe Gln Arg Ile Thr Leu 
        115                 120                 125             


Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg 
    130                 135                 140                 


Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser 
145                 150                 155                 160 


Leu Arg Ser Lys Glu 
                165 


<210>  20
<211>  495
<212>  DNA
<213>  hIFNa2

<400>  20
tgtgatctgc ctcaaaccca cagcctgggt agcaggagga ccttgatgct cctggcacag     60

atgaggagaa tctctctttt ctcctgcttg aaggacagac atgactttgg atttccccag    120

gaggagtttg gcaaccagtt ccaaaaggct gaaaccatcc ctgtcctcca tgagatgatc    180

cagcagatct tcaatctctt cagcacaaag gactcatctg ctgcttggga tgagaccctc    240

ctagacaaat tctacactga actctaccag cagctgaatg acctggaagc ctgtgtgata    300

cagggggtgg gggtgacaga gactcccctg atgaaggagg actccattct ggctgtgagg    360

aaatacttcc aaagaatcac tctctatctg aaagagaaga aatacagccc ttgtgcctgg    420

gaggttgtca gagcagaaat catgagatct ttttctttgt caacaaactt gcaagaaagt    480

ttaagaagta aggaa                                                     495


<210>  21
<211>  166
<212>  PRT
<213>  hIFNb

<400>  21

Met Ser Tyr Asn Leu Leu Gly Phe Leu Gln Arg Ser Ser Asn Phe Gln 
1               5                   10                  15      


Cys Gln Lys Leu Leu Trp Gln Leu Asn Gly Arg Leu Glu Tyr Cys Leu 
            20                  25                  30          


Lys Asp Arg Met Asn Phe Asp Ile Pro Glu Glu Ile Lys Gln Leu Gln 
        35                  40                  45              


Gln Phe Gln Lys Glu Asp Ala Ala Leu Thr Ile Tyr Glu Met Leu Gln 
    50                  55                  60                  


Asn Ile Phe Ala Ile Phe Arg Gln Asp Ser Ser Ser Thr Gly Trp Asn 
65                  70                  75                  80  


Glu Thr Ile Val Glu Asn Leu Leu Ala Asn Val Tyr His Gln Ile Asn 
                85                  90                  95      


His Leu Lys Thr Val Leu Glu Glu Lys Leu Glu Lys Glu Asp Phe Thr 
            100                 105                 110         


Arg Gly Lys Leu Met Ser Ser Leu His Leu Lys Arg Tyr Tyr Gly Arg 
        115                 120                 125             


Ile Leu His Tyr Leu Lys Ala Lys Glu Tyr Ser His Cys Ala Trp Thr 
    130                 135                 140                 


Ile Val Arg Val Glu Ile Leu Arg Asn Phe Tyr Phe Ile Asn Arg Leu 
145                 150                 155                 160 


Thr Gly Tyr Leu Arg Asn 
                165     


<210>  22
<211>  498
<212>  DNA
<213>  hIFNb

<400>  22
atgagctaca acttgcttgg attcctacaa agaagcagca attttcagtg tcagaagctc     60

ctgtggcaat tgaatgggag gcttgaatac tgcctcaagg acaggatgaa ctttgacatc    120

cctgaggaga ttaagcagct gcagcagttc cagaaggagg acgccgcatt gaccatctat    180

gagatgctcc agaacatctt tgctattttc agacaagatt catctagcac tggctggaat    240

gagactattg ttgagaacct cctggctaat gtctatcatc agataaacca tctgaagaca    300

gtcctggaag aaaaactgga gaaagaagat ttcaccaggg gaaaactcat gagcagtctg    360

cacctgaaaa gatattatgg gaggattctg cattacctga aggccaagga gtacagtcac    420

tgtgcctgga ccatagtcag agtggaaatc ctaaggaact tttacttcat taacagactt    480

acaggttacc tccgaaac                                                  498


<210>  23
<211>  133
<212>  PRT
<213>  mIFNð

<400>  23

His Gly Thr Val Ile Glu Ser Leu Glu Ser Leu Asn Asn Tyr Phe Asn 
1               5                   10                  15      


Ser Ser Gly Ile Asp Val Glu Glu Lys Ser Leu Phe Leu Asp Ile Trp 
            20                  25                  30          


Arg Asn Trp Gln Lys Asp Gly Asp Met Lys Ile Leu Gln Ser Gln Ile 
        35                  40                  45              


Ile Ser Phe Tyr Leu Arg Leu Phe Glu Val Leu Lys Asp Asn Gln Ala 
    50                  55                  60                  


Ile Ser Asn Asn Ile Ser Val Ile Glu Ser His Leu Ile Thr Thr Phe 
65                  70                  75                  80  


Phe Ser Asn Ser Lys Ala Lys Lys Asp Ala Phe Met Ser Ile Ala Lys 
                85                  90                  95      


Phe Glu Val Asn Asn Pro Gln Val Gln Arg Gln Ala Phe Asn Glu Leu 
            100                 105                 110         


Ile Arg Val Val His Gln Leu Leu Pro Glu Ser Ser Leu Arg Lys Arg 
        115                 120                 125             


Lys Arg Ser Arg Cys 
    130             


<210>  24
<211>  399
<212>  DNA
<213>  mIFNñ

<400>  24
cacggcacag tcattgaaag cctagaaagt ctgaataact attttaactc aagtggcata     60

gatgtggaag aaaagagtct cttcttggat atctggagga actggcaaaa ggatggtgac    120

atgaaaatcc tgcagagcca gattatctct ttctacctca gactctttga agtcttgaaa    180

gacaatcagg ccatcagcaa caacataagc gtcattgaat cacacctgat tactaccttc    240

ttcagcaaca gcaaggcgaa aaaggatgca ttcatgagta ttgccaagtt tgaggtcaac    300

aacccacagg tccagcgcca agcattcaat gagctcatcc gagtggtcca ccagctgttg    360

ccggaatcca gcctcaggaa gcggaaaagg agtcgctgc                           399


<210>  25
<211>  162
<212>  PRT
<213>  Mouse IFN4(L30A)

<400>  25

Cys Asp Leu Pro His Thr Tyr Asn Leu Gly Asn Lys Arg Ala Leu Thr 
1               5                   10                  15      


Val Leu Glu Glu Met Arg Arg Leu Pro Pro Leu Ser Cys Ala Lys Asp 
            20                  25                  30          


Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys Val Asp Asn Gln Gln Ile 
        35                  40                  45              


Gln Lys Ala Gln Ala Ile Leu Val Leu Arg Asp Leu Thr Gln Gln Ile 
    50                  55                  60                  


Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser Ala Thr Trp Asn Ala Thr 
65                  70                  75                  80  


Leu Leu Asp Ser Phe Cys Asn Asp Leu His Gln Gln Leu Asn Asp Leu 
                85                  90                  95      


Lys Ala Cys Val Met Gln Glu Pro Pro Leu Thr Gln Glu Asp Ser Leu 
            100                 105                 110         


Leu Ala Val Arg Thr Tyr Phe His Arg Ile Thr Val Tyr Leu Arg Lys 
        115                 120                 125             


Lys Lys His Ser Leu Cys Ala Trp Glu Val Ile Arg Ala Glu Val Trp 
    130                 135                 140                 


Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu Ala Arg Leu Ser Glu Glu 
145                 150                 155                 160 


Lys Glu 
        


<210>  26
<211>  486
<212>  DNA
<213>  Mouse IFN4(L30A)

<400>  26
tgtgacctgc ctcacactta taacctcggg aacaagaggg ccttgacagt cctggaagaa     60

atgagaagac tcccccctct ttcctgcgcg aaggacagga aggattttgg attccccttg    120

gagaaggtgg ataaccaaca gatccagaag gctcaagcca tccttgtgct aagagatctt    180

acccagcaga ttttgaacct cttcacatca aaagacttgt ctgctacttg gaatgcaact    240

ctcctagact cattctgcaa tgacctccat cagcagctca atgatctcaa agcctgtgtg    300

atgcaggaac ctcctctgac ccaggaagac tccctgctgg ctgtgaggac atacttccac    360

aggatcactg tgtacctgag aaagaagaaa cacagcctct gtgcctggga ggtgatcaga    420

gcagaagtct ggagagccct ctcttcctca accaacttgc tggcaagact gagtgaggag    480

aaggag                                                               486


<210>  27
<211>  162
<212>  PRT
<213>  Mouse IFN4(R144A)

<400>  27

Cys Asp Leu Pro His Thr Tyr Asn Leu Gly Asn Lys Arg Ala Leu Thr 
1               5                   10                  15      


Val Leu Glu Glu Met Arg Arg Leu Pro Pro Leu Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys Val Asp Asn Gln Gln Ile 
        35                  40                  45              


Gln Lys Ala Gln Ala Ile Leu Val Leu Arg Asp Leu Thr Gln Gln Ile 
    50                  55                  60                  


Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser Ala Thr Trp Asn Ala Thr 
65                  70                  75                  80  


Leu Leu Asp Ser Phe Cys Asn Asp Leu His Gln Gln Leu Asn Asp Leu 
                85                  90                  95      


Lys Ala Cys Val Met Gln Glu Pro Pro Leu Thr Gln Glu Asp Ser Leu 
            100                 105                 110         


Leu Ala Val Arg Thr Tyr Phe His Arg Ile Thr Val Tyr Leu Arg Lys 
        115                 120                 125             


Lys Lys His Ser Leu Cys Ala Trp Glu Val Ile Ala Ala Glu Val Trp 
    130                 135                 140                 


Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu Ala Arg Leu Ser Glu Glu 
145                 150                 155                 160 


Lys Glu 
        


<210>  28
<211>  486
<212>  DNA
<213>  Mouse IFN4(R144A)

<400>  28
tgtgacctgc ctcacactta taacctcggg aacaagaggg ccttgacagt cctggaagaa     60

atgagaagac tcccccctct ttcctgcctg aaggacagga aggattttgg attccccttg    120

gagaaggtgg ataaccaaca gatccagaag gctcaagcca tccttgtgct aagagatctt    180

acccagcaga ttttgaacct cttcacatca aaagacttgt ctgctacttg gaatgcaact    240

ctcctagact cattctgcaa tgacctccat cagcagctca atgatctcaa agcctgtgtg    300

atgcaggaac ctcctctgac ccaggaagac tccctgctgg ctgtgaggac atacttccac    360

aggatcactg tgtacctgag aaagaagaaa cacagcctct gtgcctggga ggtgatcgca    420

gcagaagtct ggagagccct ctcttcctca accaacttgc tggcaagact gagtgaggag    480

aaggag                                                               486


<210>  29
<211>  162
<212>  PRT
<213>  Mouse IFN4(A145G)

<400>  29

Cys Asp Leu Pro His Thr Tyr Asn Leu Gly Asn Lys Arg Ala Leu Thr 
1               5                   10                  15      


Val Leu Glu Glu Met Arg Arg Leu Pro Pro Leu Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys Val Asp Asn Gln Gln Ile 
        35                  40                  45              


Gln Lys Ala Gln Ala Ile Leu Val Leu Arg Asp Leu Thr Gln Gln Ile 
    50                  55                  60                  


Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser Ala Thr Trp Asn Ala Thr 
65                  70                  75                  80  


Leu Leu Asp Ser Phe Cys Asn Asp Leu His Gln Gln Leu Asn Asp Leu 
                85                  90                  95      


Lys Ala Cys Val Met Gln Glu Pro Pro Leu Thr Gln Glu Asp Ser Leu 
            100                 105                 110         


Leu Ala Val Arg Thr Tyr Phe His Arg Ile Thr Val Tyr Leu Arg Lys 
        115                 120                 125             


Lys Lys His Ser Leu Cys Ala Trp Glu Val Ile Arg Gly Glu Val Trp 
    130                 135                 140                 


Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu Ala Arg Leu Ser Glu Glu 
145                 150                 155                 160 


Lys Glu 
        


<210>  30
<211>  486
<212>  DNA
<213>  Mouse IFN4(A145G)

<400>  30
tgtgacctgc ctcacactta taacctcggg aacaagaggg ccttgacagt cctggaagaa     60

atgagaagac tcccccctct ttcctgcctg aaggacagga aggattttgg attccccttg    120

gagaaggtgg ataaccaaca gatccagaag gctcaagcca tccttgtgct aagagatctt    180

acccagcaga ttttgaacct cttcacatca aaagacttgt ctgctacttg gaatgcaact    240

ctcctagact cattctgcaa tgacctccat cagcagctca atgatctcaa agcctgtgtg    300

atgcaggaac ctcctctgac ccaggaagac tccctgctgg ctgtgaggac atacttccac    360

aggatcactg tgtacctgag aaagaagaaa cacagcctct gtgcctggga ggtgatcaga    420

ggagaagtct ggagagccct ctcttcctca accaacttgc tggcaagact gagtgaggag    480

aaggag                                                               486


<210>  31
<211>  162
<212>  PRT
<213>  Mouse IFN4(R149A)

<400>  31

Cys Asp Leu Pro His Thr Tyr Asn Leu Gly Asn Lys Arg Ala Leu Thr 
1               5                   10                  15      


Val Leu Glu Glu Met Arg Arg Leu Pro Pro Leu Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys Val Asp Asn Gln Gln Ile 
        35                  40                  45              


Gln Lys Ala Gln Ala Ile Leu Val Leu Arg Asp Leu Thr Gln Gln Ile 
    50                  55                  60                  


Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser Ala Thr Trp Asn Ala Thr 
65                  70                  75                  80  


Leu Leu Asp Ser Phe Cys Asn Asp Leu His Gln Gln Leu Asn Asp Leu 
                85                  90                  95      


Lys Ala Cys Val Met Gln Glu Pro Pro Leu Thr Gln Glu Asp Ser Leu 
            100                 105                 110         


Leu Ala Val Arg Thr Tyr Phe His Arg Ile Thr Val Tyr Leu Arg Lys 
        115                 120                 125             


Lys Lys His Ser Leu Cys Ala Trp Glu Val Ile Arg Ala Glu Val Trp 
    130                 135                 140                 


Ala Ala Leu Ser Ser Ser Thr Asn Leu Leu Ala Arg Leu Ser Glu Glu 
145                 150                 155                 160 


Lys Glu 
        


<210>  32
<211>  486
<212>  DNA
<213>  Mouse IFN4(R149A)

<400>  32
tgtgacctgc ctcacactta taacctcggg aacaagaggg ccttgacagt cctggaagaa     60

atgagaagac tcccccctct ttcctgcctg aaggacagga aggattttgg attccccttg    120

gagaaggtgg ataaccaaca gatccagaag gctcaagcca tccttgtgct aagagatctt    180

acccagcaga ttttgaacct cttcacatca aaagacttgt ctgctacttg gaatgcaact    240

ctcctagact cattctgcaa tgacctccat cagcagctca atgatctcaa agcctgtgtg    300

atgcaggaac ctcctctgac ccaggaagac tccctgctgg ctgtgaggac atacttccac    360

aggatcactg tgtacctgag aaagaagaaa cacagcctct gtgcctggga ggtgatcaga    420

gcagaagtct gggcagccct ctcttcctca accaacttgc tggcaagact gagtgaggag    480

aaggag                                                               486


<210>  33
<211>  162
<212>  PRT
<213>  Mouse IFN4(S152A)

<400>  33

Cys Asp Leu Pro His Thr Tyr Asn Leu Gly Asn Lys Arg Ala Leu Thr 
1               5                   10                  15      


Val Leu Glu Glu Met Arg Arg Leu Pro Pro Leu Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys Val Asp Asn Gln Gln Ile 
        35                  40                  45              


Gln Lys Ala Gln Ala Ile Leu Val Leu Arg Asp Leu Thr Gln Gln Ile 
    50                  55                  60                  


Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser Ala Thr Trp Asn Ala Thr 
65                  70                  75                  80  


Leu Leu Asp Ser Phe Cys Asn Asp Leu His Gln Gln Leu Asn Asp Leu 
                85                  90                  95      


Lys Ala Cys Val Met Gln Glu Pro Pro Leu Thr Gln Glu Asp Ser Leu 
            100                 105                 110         


Leu Ala Val Arg Thr Tyr Phe His Arg Ile Thr Val Tyr Leu Arg Lys 
        115                 120                 125             


Lys Lys His Ser Leu Cys Ala Trp Glu Val Ile Arg Ala Glu Val Trp 
    130                 135                 140                 


Arg Ala Leu Ala Ser Ser Thr Asn Leu Leu Ala Arg Leu Ser Glu Glu 
145                 150                 155                 160 


Lys Glu 
        


<210>  34
<211>  486
<212>  DNA
<213>  Mouse IFN4(S152A)

<400>  34
tgtgacctgc ctcacactta taacctcggg aacaagaggg ccttgacagt cctggaagaa     60

atgagaagac tcccccctct ttcctgcctg aaggacagga aggattttgg attccccttg    120

gagaaggtgg ataaccaaca gatccagaag gctcaagcca tccttgtgct aagagatctt    180

acccagcaga ttttgaacct cttcacatca aaagacttgt ctgctacttg gaatgcaact    240

ctcctagact cattctgcaa tgacctccat cagcagctca atgatctcaa agcctgtgtg    300

atgcaggaac ctcctctgac ccaggaagac tccctgctgg ctgtgaggac atacttccac    360

aggatcactg tgtacctgag aaagaagaaa cacagcctct gtgcctggga ggtgatcaga    420

gcagaagtct ggagagccct cgcatcctca accaacttgc tggcaagact gagtgaggag    480

aaggag                                                               486


<210>  35
<211>  165
<212>  PRT
<213>  Human IFN2(Q124R)

<400>  35

Cys Asp Leu Pro Gln Thr His Ser Leu Gly Ser Arg Arg Thr Leu Met 
1               5                   10                  15      


Leu Leu Ala Gln Met Arg Arg Ile Ser Leu Phe Ser Cys Leu Lys Asp 
            20                  25                  30          


Arg His Asp Phe Gly Phe Pro Gln Glu Glu Phe Gly Asn Gln Phe Gln 
        35                  40                  45              


Lys Ala Glu Thr Ile Pro Val Leu His Glu Met Ile Gln Gln Ile Phe 
    50                  55                  60                  


Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala Ala Trp Asp Glu Thr Leu 
65                  70                  75                  80  


Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln Gln Leu Asn Asp Leu Glu 
                85                  90                  95      


Ala Cys Val Ile Gln Gly Val Gly Val Thr Glu Thr Pro Leu Met Lys 
            100                 105                 110         


Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr Phe Arg Arg Ile Thr Leu 
        115                 120                 125             


Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys Ala Trp Glu Val Val Arg 
    130                 135                 140                 


Ala Glu Ile Met Arg Ser Phe Ser Leu Ser Thr Asn Leu Gln Glu Ser 
145                 150                 155                 160 


Leu Arg Ser Lys Glu 
                165 


<210>  36
<211>  495
<212>  DNA
<213>  Human IFN2(Q124R)

<400>  36
tgcgatttac cccagacaca ctctttaggc tctcgtagga ccctcatgct gctggcccag     60

atgaggagga tctctttatt ctcttgttta aaggacagac acgacttcgg cttcccccaa    120

gaagagttcg gcaaccagtt ccagaaggct gagaccatcc ccgtgctgca cgagatgatc    180

cagcagatct tcaatttatt tagcaccaag gactccagcg ccgcttggga tgagacttta    240

ctggacaagt tctacaccga actgtaccag cagctgaacg atttagaggc ttgtgtgatc    300

caaggtgtgg gagtgaccga gacccctctg atgaaggagg actccatttt agccgtgagg    360

aagtactttc gtaggatcac cctctacctc aaggagaaga agtacagccc ttgcgcttgg    420

gaggtggtga gggctgagat tatgaggagc ttctctttat ccaccaatct gcaagaatct    480

ttaaggtcca aggaa                                                     495


<210>  37
<211>  435
<212>  PRT
<213>  ԴmIFNa4(L30A)-Fc-holeİ

<400>  37

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Arg Cys Asp Leu Pro His Thr Tyr Asn Leu Gly 
            20                  25                  30          


Asn Lys Arg Ala Leu Thr Val Leu Glu Glu Met Arg Arg Leu Pro Pro 
        35                  40                  45              


Leu Ser Cys Ala Lys Asp Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys 
    50                  55                  60                  


Val Asp Asn Gln Gln Ile Gln Lys Ala Gln Ala Ile Leu Val Leu Arg 
65                  70                  75                  80  


Asp Leu Thr Gln Gln Ile Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser 
                85                  90                  95      


Ala Thr Trp Asn Ala Thr Leu Leu Asp Ser Phe Cys Asn Asp Leu His 
            100                 105                 110         


Gln Gln Leu Asn Asp Leu Lys Ala Cys Val Met Gln Glu Pro Pro Leu 
        115                 120                 125             


Thr Gln Glu Asp Ser Leu Leu Ala Val Arg Thr Tyr Phe His Arg Ile 
    130                 135                 140                 


Thr Val Tyr Leu Arg Lys Lys Lys His Ser Leu Cys Ala Trp Glu Val 
145                 150                 155                 160 


Ile Arg Ala Glu Val Trp Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu 
                165                 170                 175     


Ala Arg Leu Ser Glu Glu Lys Glu Ser Gly Gly Gly Gly Ser Gly Gly 
            180                 185                 190         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Arg Thr Asp Lys 
        195                 200                 205             


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
    210                 215                 220                 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
225                 230                 235                 240 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
                245                 250                 255     


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
            260                 265                 270         


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
        275                 280                 285             


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
    290                 295                 300                 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
305                 310                 315                 320 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr 
                325                 330                 335     


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser 
            340                 345                 350         


Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
        355                 360                 365             


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
    370                 375                 380                 


Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys 
385                 390                 395                 400 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
                405                 410                 415     


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
            420                 425                 430         


Lys His Val 
        435 


<210>  38
<211>  1311
<212>  DNA
<213>  ԴmIFNa4(L30A)-Fc-holeı

<400>  38
gccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt     60

tccactggtt cgcgatgtga cctgcctcac acttataacc tcgggaacaa gagggccttg    120

acagtcctgg aagaaatgag aagactcccc cctctttcct gcgcgaagga caggaaggat    180

tttggattcc ccttggagaa ggtggataac caacagatcc agaaggctca agccatcctt    240

gtgctaagag atcttaccca gcagattttg aacctcttca catcaaaaga cttgtctgct    300

acttggaatg caactctcct agactcattc tgcaatgacc tccatcagca gctcaatgat    360

ctcaaagcct gtgtgatgca ggaacctcct ctgacccagg aagactccct gctggctgtg    420

aggacatact tccacaggat cactgtgtac ctgagaaaga agaaacacag cctctgtgcc    480

tgggaggtga tcagagcaga agtctggaga gccctctctt cctcaaccaa cttgctggca    540

agactgagtg aggagaagga gagtggcggt ggtggctccg gcggtggtgg ctccggcggt    600

ggtggctccg gcggtggtgg ccgtacggac aagacccaca cctgcccccc ctgccccgcc    660

cccgagctgc tgggcggccc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg    720

atgatcagcc gcacccccga ggtgacctgc gtggtggtgg acgtgagcca cgaggacccc    780

gaggtgaagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa gaccaagccc    840

cgcgaggagc agtacaacag cacctaccgc gtggtgagcg tgctgaccgt gctgcaccag    900

gactggctga acggcaagga gtacaagtgc aaggtgagca acaaggccct gcccgccccc    960

atcgagaaga ccatcagcaa ggccaagggc cagccccgcg agccccaggt gtgcaccctg   1020

ccccccagcc gcgacgagct gaccaagaac caggtgagcc tgagctgcgc cgtgaagggc   1080

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga gaacaactac   1140

aagaccaccc cccccgtgct ggacagcgac ggcagcttct tcctggtgag caagctgacc   1200

gtggacaaga gccgctggca gcagggcaac gtgttcagct gcagcgtgat gcacgaggcc   1260

ctgcacaacc actacaccca gaagagcctg agcctgagcc ccggcaagta a            1311


<210>  39
<211>  435
<212>  PRT
<213>  ԴmIFN4 (R144A)-Fc-holeİ

<400>  39

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Arg Cys Asp Leu Pro His Thr Tyr Asn Leu Gly 
            20                  25                  30          


Asn Lys Arg Ala Leu Thr Val Leu Glu Glu Met Arg Arg Leu Pro Pro 
        35                  40                  45              


Leu Ser Cys Leu Lys Asp Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys 
    50                  55                  60                  


Val Asp Asn Gln Gln Ile Gln Lys Ala Gln Ala Ile Leu Val Leu Arg 
65                  70                  75                  80  


Asp Leu Thr Gln Gln Ile Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser 
                85                  90                  95      


Ala Thr Trp Asn Ala Thr Leu Leu Asp Ser Phe Cys Asn Asp Leu His 
            100                 105                 110         


Gln Gln Leu Asn Asp Leu Lys Ala Cys Val Met Gln Glu Pro Pro Leu 
        115                 120                 125             


Thr Gln Glu Asp Ser Leu Leu Ala Val Arg Thr Tyr Phe His Arg Ile 
    130                 135                 140                 


Thr Val Tyr Leu Arg Lys Lys Lys His Ser Leu Cys Ala Trp Glu Val 
145                 150                 155                 160 


Ile Ala Ala Glu Val Trp Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu 
                165                 170                 175     


Ala Arg Leu Ser Glu Glu Lys Glu Ser Gly Gly Gly Gly Ser Gly Gly 
            180                 185                 190         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Arg Thr Asp Lys 
        195                 200                 205             


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
    210                 215                 220                 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
225                 230                 235                 240 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
                245                 250                 255     


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
            260                 265                 270         


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
        275                 280                 285             


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
    290                 295                 300                 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
305                 310                 315                 320 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr 
                325                 330                 335     


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser 
            340                 345                 350         


Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
        355                 360                 365             


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
    370                 375                 380                 


Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys 
385                 390                 395                 400 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
                405                 410                 415     


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
            420                 425                 430         


Lys His Val 
        435 


<210>  40
<211>  1311
<212>  DNA
<213>  ԴmIFNa4(R144A)-Fc-holeı

<400>  40
gccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt     60

tccactggtt cgcgatgtga cctgcctcac acttataacc tcgggaacaa gagggccttg    120

acagtcctgg aagaaatgag aagactcccc cctctttcct gcctgaagga caggaaggat    180

tttggattcc ccttggagaa ggtggataac caacagatcc agaaggctca agccatcctt    240

gtgctaagag atcttaccca gcagattttg aacctcttca catcaaaaga cttgtctgct    300

acttggaatg caactctcct agactcattc tgcaatgacc tccatcagca gctcaatgat    360

ctcaaagcct gtgtgatgca ggaacctcct ctgacccagg aagactccct gctggctgtg    420

aggacatact tccacaggat cactgtgtac ctgagaaaga agaaacacag cctctgtgcc    480

tgggaggtga tcgcagcaga agtctggaga gccctctctt cctcaaccaa cttgctggca    540

agactgagtg aggagaagga gagtggcggt ggtggctccg gcggtggtgg ctccggcggt    600

ggtggctccg gcggtggtgg ccgtacggac aagacccaca cctgcccccc ctgccccgcc    660

cccgagctgc tgggcggccc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg    720

atgatcagcc gcacccccga ggtgacctgc gtggtggtgg acgtgagcca cgaggacccc    780

gaggtgaagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa gaccaagccc    840

cgcgaggagc agtacaacag cacctaccgc gtggtgagcg tgctgaccgt gctgcaccag    900

gactggctga acggcaagga gtacaagtgc aaggtgagca acaaggccct gcccgccccc    960

atcgagaaga ccatcagcaa ggccaagggc cagccccgcg agccccaggt gtgcaccctg   1020

ccccccagcc gcgacgagct gaccaagaac caggtgagcc tgagctgcgc cgtgaagggc   1080

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga gaacaactac   1140

aagaccaccc cccccgtgct ggacagcgac ggcagcttct tcctggtgag caagctgacc   1200

gtggacaaga gccgctggca gcagggcaac gtgttcagct gcagcgtgat gcacgaggcc   1260

ctgcacaacc actacaccca gaagagcctg agcctgagcc ccggcaagta a            1311


<210>  41
<211>  435
<212>  PRT
<213>  ԴmIFN4 (A145G)-Fc-holeİ

<400>  41

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Arg Cys Asp Leu Pro His Thr Tyr Asn Leu Gly 
            20                  25                  30          


Asn Lys Arg Ala Leu Thr Val Leu Glu Glu Met Arg Arg Leu Pro Pro 
        35                  40                  45              


Leu Ser Cys Leu Lys Asp Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys 
    50                  55                  60                  


Val Asp Asn Gln Gln Ile Gln Lys Ala Gln Ala Ile Leu Val Leu Arg 
65                  70                  75                  80  


Asp Leu Thr Gln Gln Ile Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser 
                85                  90                  95      


Ala Thr Trp Asn Ala Thr Leu Leu Asp Ser Phe Cys Asn Asp Leu His 
            100                 105                 110         


Gln Gln Leu Asn Asp Leu Lys Ala Cys Val Met Gln Glu Pro Pro Leu 
        115                 120                 125             


Thr Gln Glu Asp Ser Leu Leu Ala Val Arg Thr Tyr Phe His Arg Ile 
    130                 135                 140                 


Thr Val Tyr Leu Arg Lys Lys Lys His Ser Leu Cys Ala Trp Glu Val 
145                 150                 155                 160 


Ile Arg Gly Glu Val Trp Arg Ala Leu Ser Ser Ser Thr Asn Leu Leu 
                165                 170                 175     


Ala Arg Leu Ser Glu Glu Lys Glu Ser Gly Gly Gly Gly Ser Gly Gly 
            180                 185                 190         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Arg Thr Asp Lys 
        195                 200                 205             


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
    210                 215                 220                 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
225                 230                 235                 240 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
                245                 250                 255     


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
            260                 265                 270         


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
        275                 280                 285             


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
    290                 295                 300                 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
305                 310                 315                 320 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr 
                325                 330                 335     


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser 
            340                 345                 350         


Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
        355                 360                 365             


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
    370                 375                 380                 


Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys 
385                 390                 395                 400 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
                405                 410                 415     


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
            420                 425                 430         


Lys His Val 
        435 


<210>  42
<211>  1311
<212>  DNA
<213>  ԴmIFNa4(A145G)-Fc-holeı

<400>  42
gccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt     60

tccactggtt cgcgatgtga cctgcctcac acttataacc tcgggaacaa gagggccttg    120

acagtcctgg aagaaatgag aagactcccc cctctttcct gcctgaagga caggaaggat    180

tttggattcc ccttggagaa ggtggataac caacagatcc agaaggctca agccatcctt    240

gtgctaagag atcttaccca gcagattttg aacctcttca catcaaaaga cttgtctgct    300

acttggaatg caactctcct agactcattc tgcaatgacc tccatcagca gctcaatgat    360

ctcaaagcct gtgtgatgca ggaacctcct ctgacccagg aagactccct gctggctgtg    420

aggacatact tccacaggat cactgtgtac ctgagaaaga agaaacacag cctctgtgcc    480

tgggaggtga tcagaggaga agtctggaga gccctctctt cctcaaccaa cttgctggca    540

agactgagtg aggagaagga gagtggcggt ggtggctccg gcggtggtgg ctccggcggt    600

ggtggctccg gcggtggtgg ccgtacggac aagacccaca cctgcccccc ctgccccgcc    660

cccgagctgc tgggcggccc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg    720

atgatcagcc gcacccccga ggtgacctgc gtggtggtgg acgtgagcca cgaggacccc    780

gaggtgaagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa gaccaagccc    840

cgcgaggagc agtacaacag cacctaccgc gtggtgagcg tgctgaccgt gctgcaccag    900

gactggctga acggcaagga gtacaagtgc aaggtgagca acaaggccct gcccgccccc    960

atcgagaaga ccatcagcaa ggccaagggc cagccccgcg agccccaggt gtgcaccctg   1020

ccccccagcc gcgacgagct gaccaagaac caggtgagcc tgagctgcgc cgtgaagggc   1080

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga gaacaactac   1140

aagaccaccc cccccgtgct ggacagcgac ggcagcttct tcctggtgag caagctgacc   1200

gtggacaaga gccgctggca gcagggcaac gtgttcagct gcagcgtgat gcacgaggcc   1260

ctgcacaacc actacaccca gaagagcctg agcctgagcc ccggcaagta a            1311


<210>  43
<211>  436
<212>  PRT
<213>  ԴmIFN4 (R149A)-Fc-holeİ

<400>  43

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Arg Cys Asp Leu Pro His Thr Tyr Asn Leu Gly 
            20                  25                  30          


Asn Lys Arg Ala Leu Thr Val Leu Glu Glu Met Arg Arg Leu Pro Pro 
        35                  40                  45              


Leu Ser Cys Leu Lys Asp Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys 
    50                  55                  60                  


Val Asp Asn Gln Gln Ile Gln Lys Ala Gln Ala Ile Leu Val Leu Arg 
65                  70                  75                  80  


Asp Leu Thr Gln Gln Ile Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser 
                85                  90                  95      


Ala Thr Trp Asn Ala Thr Leu Leu Asp Ser Phe Cys Asn Asp Leu His 
            100                 105                 110         


Gln Gln Leu Asn Asp Leu Lys Ala Cys Val Met Gln Glu Pro Pro Leu 
        115                 120                 125             


Thr Gln Glu Asp Ser Leu Leu Ala Val Arg Thr Tyr Phe His Arg Ile 
    130                 135                 140                 


Thr Val Tyr Leu Arg Lys Lys Lys His Ser Leu Cys Ala Trp Glu Val 
145                 150                 155                 160 


Ile Arg Ala Glu Val Trp Ala Ala Leu Ser Ser Ser Thr Asn Leu Leu 
                165                 170                 175     


Ala Arg Leu Ser Glu Glu Lys Glu Ser Ser Gly Gly Gly Gly Ser Gly 
            180                 185                 190         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Arg Thr Asp 
        195                 200                 205             


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
    210                 215                 220                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
225                 230                 235                 240 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                245                 250                 255     


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            260                 265                 270         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
        275                 280                 285             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    290                 295                 300                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
305                 310                 315                 320 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys 
                325                 330                 335     


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
            340                 345                 350         


Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        355                 360                 365             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    370                 375                 380                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp 
385                 390                 395                 400 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                405                 410                 415     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            420                 425                 430         


Gly Lys His Val 
        435     


<210>  44
<211>  1311
<212>  DNA
<213>  ԴmIFNa4(R149A)-Fc-holeı

<400>  44
gccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt     60

tccactggtt cgcgatgtga cctgcctcac acttataacc tcgggaacaa gagggccttg    120

acagtcctgg aagaaatgag aagactcccc cctctttcct gcctgaagga caggaaggat    180

tttggattcc ccttggagaa ggtggataac caacagatcc agaaggctca agccatcctt    240

gtgctaagag atcttaccca gcagattttg aacctcttca catcaaaaga cttgtctgct    300

acttggaatg caactctcct agactcattc tgcaatgacc tccatcagca gctcaatgat    360

ctcaaagcct gtgtgatgca ggaacctcct ctgacccagg aagactccct gctggctgtg    420

aggacatact tccacaggat cactgtgtac ctgagaaaga agaaacacag cctctgtgcc    480

tgggaggtga tcagagcaga agtctgggca gccctctctt cctcaaccaa cttgctggca    540

agactgagtg aggagaagga gagtggcggt ggtggctccg gcggtggtgg ctccggcggt    600

ggtggctccg gcggtggtgg ccgtacggac aagacccaca cctgcccccc ctgccccgcc    660

cccgagctgc tgggcggccc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg    720

atgatcagcc gcacccccga ggtgacctgc gtggtggtgg acgtgagcca cgaggacccc    780

gaggtgaagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa gaccaagccc    840

cgcgaggagc agtacaacag cacctaccgc gtggtgagcg tgctgaccgt gctgcaccag    900

gactggctga acggcaagga gtacaagtgc aaggtgagca acaaggccct gcccgccccc    960

atcgagaaga ccatcagcaa ggccaagggc cagccccgcg agccccaggt gtgcaccctg   1020

ccccccagcc gcgacgagct gaccaagaac caggtgagcc tgagctgcgc cgtgaagggc   1080

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga gaacaactac   1140

aagaccaccc cccccgtgct ggacagcgac ggcagcttct tcctggtgag caagctgacc   1200

gtggacaaga gccgctggca gcagggcaac gtgttcagct gcagcgtgat gcacgaggcc   1260

ctgcacaacc actacaccca gaagagcctg agcctgagcc ccggcaagta a            1311


<210>  45
<211>  435
<212>  PRT
<213>  ԴmIFN4 (S152A)-Fc-holeİ

<400>  45

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Arg Cys Asp Leu Pro His Thr Tyr Asn Leu Gly 
            20                  25                  30          


Asn Lys Arg Ala Leu Thr Val Leu Glu Glu Met Arg Arg Leu Pro Pro 
        35                  40                  45              


Leu Ser Cys Leu Lys Asp Arg Lys Asp Phe Gly Phe Pro Leu Glu Lys 
    50                  55                  60                  


Val Asp Asn Gln Gln Ile Gln Lys Ala Gln Ala Ile Leu Val Leu Arg 
65                  70                  75                  80  


Asp Leu Thr Gln Gln Ile Leu Asn Leu Phe Thr Ser Lys Asp Leu Ser 
                85                  90                  95      


Ala Thr Trp Asn Ala Thr Leu Leu Asp Ser Phe Cys Asn Asp Leu His 
            100                 105                 110         


Gln Gln Leu Asn Asp Leu Lys Ala Cys Val Met Gln Glu Pro Pro Leu 
        115                 120                 125             


Thr Gln Glu Asp Ser Leu Leu Ala Val Arg Thr Tyr Phe His Arg Ile 
    130                 135                 140                 


Thr Val Tyr Leu Arg Lys Lys Lys His Ser Leu Cys Ala Trp Glu Val 
145                 150                 155                 160 


Ile Arg Ala Glu Val Trp Ala Ala Leu Ser Ser Ser Thr Asn Leu Leu 
                165                 170                 175     


Ala Arg Leu Ser Glu Glu Lys Glu Ser Gly Gly Gly Gly Ser Gly Gly 
            180                 185                 190         


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Arg Thr Asp Lys 
        195                 200                 205             


Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro 
    210                 215                 220                 


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser 
225                 230                 235                 240 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
                245                 250                 255     


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
            260                 265                 270         


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
        275                 280                 285             


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
    290                 295                 300                 


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
305                 310                 315                 320 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr 
                325                 330                 335     


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser 
            340                 345                 350         


Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
        355                 360                 365             


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
    370                 375                 380                 


Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys 
385                 390                 395                 400 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
                405                 410                 415     


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
            420                 425                 430         


Lys His Val 
        435 


<210>  46
<211>  1311
<212>  DNA
<213>  ԴmIFNa4(S152A)-Fc-holeı

<400>  46
gccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt     60

tccactggtt cgcgatgtga cctgcctcac acttataacc tcgggaacaa gagggccttg    120

acagtcctgg aagaaatgag aagactcccc cctctttcct gcctgaagga caggaaggat    180

tttggattcc ccttggagaa ggtggataac caacagatcc agaaggctca agccatcctt    240

gtgctaagag atcttaccca gcagattttg aacctcttca catcaaaaga cttgtctgct    300

acttggaatg caactctcct agactcattc tgcaatgacc tccatcagca gctcaatgat    360

ctcaaagcct gtgtgatgca ggaacctcct ctgacccagg aagactccct gctggctgtg    420

aggacatact tccacaggat cactgtgtac ctgagaaaga agaaacacag cctctgtgcc    480

tgggaggtga tcagagcaga agtctgggca gccctctctt cctcaaccaa cttgctggca    540

agactgagtg aggagaagga gagtggcggt ggtggctccg gcggtggtgg ctccggcggt    600

ggtggctccg gcggtggtgg ccgtacggac aagacccaca cctgcccccc ctgccccgcc    660

cccgagctgc tgggcggccc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg    720

atgatcagcc gcacccccga ggtgacctgc gtggtggtgg acgtgagcca cgaggacccc    780

gaggtgaagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa gaccaagccc    840

cgcgaggagc agtacaacag cacctaccgc gtggtgagcg tgctgaccgt gctgcaccag    900

gactggctga acggcaagga gtacaagtgc aaggtgagca acaaggccct gcccgccccc    960

atcgagaaga ccatcagcaa ggccaagggc cagccccgcg agccccaggt gtgcaccctg   1020

ccccccagcc gcgacgagct gaccaagaac caggtgagcc tgagctgcgc cgtgaagggc   1080

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga gaacaactac   1140

aagaccaccc cccccgtgct ggacagcgac ggcagcttct tcctggtgag caagctgacc   1200

gtggacaaga gccgctggca gcagggcaac gtgttcagct gcagcgtgat gcacgaggcc   1260

ctgcacaacc actacaccca gaagagcctg agcctgagcc ccggcaagta a            1311


<210>  47
<211>  438
<212>  PRT
<213>  ԴhIFN2 (Q124R)-Fc-holeİ

<400>  47

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Arg Cys Asp Leu Pro Gln Thr His Ser Leu Gly 
            20                  25                  30          


Ser Arg Arg Thr Leu Met Leu Leu Ala Gln Met Arg Arg Ile Ser Leu 
        35                  40                  45              


Phe Ser Cys Leu Lys Asp Arg His Asp Phe Gly Phe Pro Gln Glu Glu 
    50                  55                  60                  


Phe Gly Asn Gln Phe Gln Lys Ala Glu Thr Ile Pro Val Leu His Glu 
65                  70                  75                  80  


Met Ile Gln Gln Ile Phe Asn Leu Phe Ser Thr Lys Asp Ser Ser Ala 
                85                  90                  95      


Ala Trp Asp Glu Thr Leu Leu Asp Lys Phe Tyr Thr Glu Leu Tyr Gln 
            100                 105                 110         


Gln Leu Asn Asp Leu Glu Ala Cys Val Ile Gln Gly Val Gly Val Thr 
        115                 120                 125             


Glu Thr Pro Leu Met Lys Glu Asp Ser Ile Leu Ala Val Arg Lys Tyr 
    130                 135                 140                 


Phe Arg Arg Ile Thr Leu Tyr Leu Lys Glu Lys Lys Tyr Ser Pro Cys 
145                 150                 155                 160 


Ala Trp Glu Val Val Arg Ala Glu Ile Met Arg Ser Phe Ser Leu Ser 
                165                 170                 175     


Thr Asn Leu Gln Glu Ser Leu Arg Ser Lys Glu Ser Gly Gly Gly Gly 
            180                 185                 190         


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Arg 
        195                 200                 205             


Thr Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 
    210                 215                 220                 


Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
225                 230                 235                 240 


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
                245                 250                 255     


His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 
            260                 265                 270         


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 
        275                 280                 285             


Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
    290                 295                 300                 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 
305                 310                 315                 320 


Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
                325                 330                 335     


Val Cys Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 
            340                 345                 350         


Ser Leu Ser Cys Ala Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
        355                 360                 365             


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
    370                 375                 380                 


Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr 
385                 390                 395                 400 


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
                405                 410                 415     


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
            420                 425                 430         


Ser Pro Gly Lys His Val 
        435             


<210>  48
<211>  1320
<212>  DNA
<213>  ԴhIFNa2(Q124R)-Fc-holeı

<400>  48
gccgccacca tggagacaga cacactcctg ctatgggtac tgctgctctg ggttccaggt     60

tccactggtt cgcgatgcga tttaccccag acacactctt taggctctcg taggaccctc    120

atgctgctgg cccagatgag gaggatctct ttattctctt gtttaaagga cagacacgac    180

ttcggcttcc cccaagaaga gttcggcaac cagttccaga aggctgagac catccccgtg    240

ctgcacgaga tgatccagca gatcttcaat ttatttagca ccaaggactc cagcgccgct    300

tgggatgaga ctttactgga caagttctac accgaactgt accagcagct gaacgattta    360

gaggcttgtg tgatccaagg tgtgggagtg accgagaccc ctctgatgaa ggaggactcc    420

attttagccg tgaggaagta ctttcgtagg atcaccctct acctcaagga gaagaagtac    480

agcccttgcg cttgggaggt ggtgagggct gagattatga ggagcttctc tttatccacc    540

aatctgcaag aatctttaag gtccaaggaa agtggcggtg gtggctccgg cggtggtggc    600

tccggcggtg gtggctccgg cggtggtggc cgtacggaca agacccacac ctgccccccc    660

tgccccgccc ccgagctgct gggcggcccc agcgtgttcc tgttcccccc caagcccaag    720

gacaccctga tgatcagccg cacccccgag gtgacctgcg tggtggtgga cgtgagccac    780

gaggaccccg aggtgaagtt caactggtac gtggacggcg tggaggtgca caacgccaag    840

accaagcccc gcgaggagca gtacaacagc acctaccgcg tggtgagcgt gctgaccgtg    900

ctgcaccagg actggctgaa cggcaaggag tacaagtgca aggtgagcaa caaggccctg    960

cccgccccca tcgagaagac catcagcaag gccaagggcc agccccgcga gccccaggtg   1020

tgcaccctgc cccccagccg cgacgagctg accaagaacc aggtgagcct gagctgcgcc   1080

gtgaagggct tctaccccag cgacatcgcc gtggagtggg agagcaacgg ccagcccgag   1140

aacaactaca agaccacccc ccccgtgctg gacagcgacg gcagcttctt cctggtgagc   1200

aagctgaccg tggacaagag ccgctggcag cagggcaacg tgttcagctg cagcgtgatg   1260

cacgaggccc tgcacaacca ctacacccag aagagcctga gcctgagccc cggcaagtaa   1320


