                         SEQUENCE LISTING

<110>  The Trustees of Princeton University
       Chitrakar, Alisha
       Korennykh, Alexei
       
<120>  Compositions Comprising Immune System Activators and Method of Using Same

<130>  5391.1012-002

<140>  PCT/US2019/026446
<141>  2019-04-09

<150>  62/803,058
<151>  2019-02-08

<150>  62/654,923
<151>  2018-04-09

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  741
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Glu Ser Arg Asp His Asn Asn Pro Gln Glu Gly Pro Thr Ser Ser 
1               5                   10                  15      


Ser Gly Arg Arg Ala Ala Val Glu Asp Asn His Leu Leu Ile Lys Ala 
            20                  25                  30          


Val Gln Asn Glu Asp Val Asp Leu Val Gln Gln Leu Leu Glu Gly Gly 
        35                  40                  45              


Ala Asn Val Asn Phe Gln Glu Glu Glu Gly Gly Trp Thr Pro Leu His 
    50                  55                  60                  


Asn Ala Val Gln Met Ser Arg Glu Asp Ile Val Glu Leu Leu Leu Arg 
65                  70                  75                  80  


His Gly Ala Asp Pro Val Leu Arg Lys Lys Asn Gly Ala Thr Pro Phe 
                85                  90                  95      


Ile Leu Ala Ala Ile Ala Gly Ser Val Lys Leu Leu Lys Leu Phe Leu 
            100                 105                 110         


Ser Lys Gly Ala Asp Val Asn Glu Cys Asp Phe Tyr Gly Phe Thr Ala 
        115                 120                 125             


Phe Met Glu Ala Ala Val Tyr Gly Lys Val Lys Ala Leu Lys Phe Leu 
    130                 135                 140                 


Tyr Lys Arg Gly Ala Asn Val Asn Leu Arg Arg Lys Thr Lys Glu Asp 
145                 150                 155                 160 


Gln Glu Arg Leu Arg Lys Gly Gly Ala Thr Ala Leu Met Asp Ala Ala 
                165                 170                 175     


Glu Lys Gly His Val Glu Val Leu Lys Ile Leu Leu Asp Glu Met Gly 
            180                 185                 190         


Ala Asp Val Asn Ala Cys Asp Asn Met Gly Arg Asn Ala Leu Ile His 
        195                 200                 205             


Ala Leu Leu Ser Ser Asp Asp Ser Asp Val Glu Ala Ile Thr His Leu 
    210                 215                 220                 


Leu Leu Asp His Gly Ala Asp Val Asn Val Arg Gly Glu Arg Gly Lys 
225                 230                 235                 240 


Thr Pro Leu Ile Leu Ala Val Glu Lys Lys His Leu Gly Leu Val Gln 
                245                 250                 255     


Arg Leu Leu Glu Gln Glu His Ile Glu Ile Asn Asp Thr Asp Ser Asp 
            260                 265                 270         


Gly Lys Thr Ala Leu Leu Leu Ala Val Glu Leu Lys Leu Lys Lys Ile 
        275                 280                 285             


Ala Glu Leu Leu Cys Lys Arg Gly Ala Ser Thr Asp Cys Gly Asp Leu 
    290                 295                 300                 


Val Met Thr Ala Arg Arg Asn Tyr Asp His Ser Leu Val Lys Val Leu 
305                 310                 315                 320 


Leu Ser His Gly Ala Lys Glu Asp Phe His Pro Pro Ala Glu Asp Trp 
                325                 330                 335     


Lys Pro Gln Ser Ser His Trp Gly Ala Ala Leu Lys Asp Leu His Arg 
            340                 345                 350         


Ile Tyr Arg Pro Met Ile Gly Lys Leu Lys Phe Phe Ile Asp Glu Lys 
        355                 360                 365             


Tyr Lys Ile Ala Asp Thr Ser Glu Gly Gly Ile Tyr Leu Gly Phe Tyr 
    370                 375                 380                 


Glu Lys Gln Glu Val Ala Val Lys Thr Phe Cys Glu Gly Ser Pro Arg 
385                 390                 395                 400 


Ala Gln Arg Glu Val Ser Cys Leu Gln Ser Ser Arg Glu Asn Ser His 
                405                 410                 415     


Leu Val Thr Phe Tyr Gly Ser Glu Ser His Arg Gly His Leu Phe Val 
            420                 425                 430         


Cys Val Thr Leu Cys Glu Gln Thr Leu Glu Ala Cys Leu Asp Val His 
        435                 440                 445             


Arg Gly Glu Asp Val Glu Asn Glu Glu Asp Glu Phe Ala Arg Asn Val 
    450                 455                 460                 


Leu Ser Ser Ile Phe Lys Ala Val Gln Glu Leu His Leu Ser Cys Gly 
465                 470                 475                 480 


Tyr Thr His Gln Asp Leu Gln Pro Gln Asn Ile Leu Ile Asp Ser Lys 
                485                 490                 495     


Lys Ala Ala His Leu Ala Asp Phe Asp Lys Ser Ile Lys Trp Ala Gly 
            500                 505                 510         


Asp Pro Gln Glu Val Lys Arg Asp Leu Glu Asp Leu Gly Arg Leu Val 
        515                 520                 525             


Leu Tyr Val Val Lys Lys Gly Ser Ile Ser Phe Glu Asp Leu Lys Ala 
    530                 535                 540                 


Gln Ser Asn Glu Glu Val Val Gln Leu Ser Pro Asp Glu Glu Thr Lys 
545                 550                 555                 560 


Asp Leu Ile His Arg Leu Phe His Pro Gly Glu His Val Arg Asp Cys 
                565                 570                 575     


Leu Ser Asp Leu Leu Gly His Pro Phe Phe Trp Thr Trp Glu Ser Arg 
            580                 585                 590         


Tyr Arg Thr Leu Arg Asn Val Gly Asn Glu Ser Asp Ile Lys Thr Arg 
        595                 600                 605             


Lys Ser Glu Ser Glu Ile Leu Arg Leu Leu Gln Pro Gly Pro Ser Glu 
    610                 615                 620                 


His Ser Lys Ser Phe Asp Lys Trp Thr Thr Lys Ile Asn Glu Cys Val 
625                 630                 635                 640 


Met Lys Lys Met Asn Lys Phe Tyr Glu Lys Arg Gly Asn Phe Tyr Gln 
                645                 650                 655     


Asn Thr Val Gly Asp Leu Leu Lys Phe Ile Arg Asn Leu Gly Glu His 
            660                 665                 670         


Ile Asp Glu Glu Lys His Lys Lys Met Lys Leu Lys Ile Gly Asp Pro 
        675                 680                 685             


Ser Leu Tyr Phe Gln Lys Thr Phe Pro Asp Leu Val Ile Tyr Val Tyr 
    690                 695                 700                 


Thr Lys Leu Gln Asn Thr Glu Tyr Arg Lys His Phe Pro Gln Thr His 
705                 710                 715                 720 


Ser Pro Asn Lys Pro Gln Cys Asp Gly Ala Gly Gly Ala Ser Gly Leu 
                725                 730                 735     


Ala Ser Pro Gly Cys 
            740     


<210>  2
<211>  652
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Glu Ser Arg Asp His Asn Asn Pro Gln Glu Gly Pro Thr Ser Ser 
1               5                   10                  15      


Ser Gly Arg Arg Ala Ala Val Glu Asp Asn His Leu Leu Ile Lys Ala 
            20                  25                  30          


Val Gln Asn Glu Asp Val Asp Leu Val Gln Gln Leu Leu Glu Gly Gly 
        35                  40                  45              


Ala Asn Val Asn Phe Gln Glu Glu Glu Gly Gly Trp Thr Pro Leu His 
    50                  55                  60                  


Asn Ala Val Gln Met Ser Arg Glu Asp Ile Val Glu Leu Leu Leu Arg 
65                  70                  75                  80  


His Gly Ala Asp Pro Val Leu Arg Lys Lys Asn Gly Ala Thr Pro Phe 
                85                  90                  95      


Ile Leu Ala Ala Ile Ala Gly Ser Val Lys Leu Leu Lys Leu Phe Leu 
            100                 105                 110         


Ser Lys Gly Ala Asp Val Asn Glu Cys Asp Phe Tyr Gly Phe Thr Ala 
        115                 120                 125             


Phe Met Glu Ala Ala Val Tyr Gly Lys Val Lys Ala Leu Lys Phe Leu 
    130                 135                 140                 


Tyr Lys Arg Gly Ala Asn Val Asn Leu Arg Arg Lys Thr Lys Glu Asp 
145                 150                 155                 160 


Gln Glu Arg Leu Arg Lys Gly Gly Ala Thr Ala Leu Met Asp Ala Ala 
                165                 170                 175     


Glu Lys Gly His Val Glu Val Leu Lys Ile Leu Leu Asp Glu Met Gly 
            180                 185                 190         


Ala Asp Val Asn Ala Cys Asp Asn Met Gly Arg Asn Ala Leu Ile His 
        195                 200                 205             


Ala Leu Leu Ser Ser Asp Asp Ser Asp Val Glu Ala Ile Thr His Leu 
    210                 215                 220                 


Leu Leu Asp His Gly Ala Asp Val Asn Val Arg Gly Glu Arg Gly Lys 
225                 230                 235                 240 


Thr Pro Leu Ile Leu Ala Val Glu Lys Lys His Leu Gly Leu Val Gln 
                245                 250                 255     


Arg Leu Leu Glu Gln Glu His Ile Glu Ile Asn Asp Thr Asp Ser Asp 
            260                 265                 270         


Gly Lys Thr Ala Leu Leu Leu Ala Val Glu Leu Lys Leu Lys Lys Ile 
        275                 280                 285             


Ala Glu Leu Leu Cys Lys Arg Gly Ala Ser Thr Asp Cys Gly Asp Leu 
    290                 295                 300                 


Val Met Thr Ala Arg Arg Asn Tyr Asp His Ser Leu Val Lys Val Leu 
305                 310                 315                 320 


Leu Ser His Gly Ala Lys Glu Asp Phe His Pro Pro Ala Glu Asp Trp 
                325                 330                 335     


Lys Pro Gln Ser Ser His Trp Gly Ala Ala Leu Lys Asp Leu His Arg 
            340                 345                 350         


Ile Tyr Arg Pro Met Ile Gly Lys Leu Lys Phe Phe Ile Asp Glu Lys 
        355                 360                 365             


Tyr Lys Ile Ala Asp Thr Ser Glu Gly Gly Ile Tyr Leu Gly Phe Tyr 
    370                 375                 380                 


Glu Lys Gln Glu Val Ala Val Lys Thr Phe Cys Glu Gly Ser Pro Arg 
385                 390                 395                 400 


Ala Gln Arg Glu Val Ser Cys Leu Gln Ser Ser Arg Glu Asn Ser His 
                405                 410                 415     


Leu Val Thr Phe Tyr Gly Ser Glu Ser His Arg Gly His Leu Phe Val 
            420                 425                 430         


Cys Val Thr Leu Cys Glu Gln Thr Leu Glu Ala Cys Leu Asp Val His 
        435                 440                 445             


Arg Gly Glu Asp Val Glu Asn Glu Glu Asp Glu Phe Ala Arg Asn Val 
    450                 455                 460                 


Leu Ser Ser Ile Phe Lys Ala Val Gln Glu Leu His Leu Ser Cys Gly 
465                 470                 475                 480 


Tyr Thr His Gln Asp Leu Gln Pro Gln Asn Ile Leu Ile Asp Ser Lys 
                485                 490                 495     


Lys Ala Ala His Leu Ala Asp Phe Asp Lys Ser Ile Lys Trp Ala Gly 
            500                 505                 510         


Asp Pro Gln Glu Val Lys Arg Asp Leu Glu Asp Leu Gly Arg Leu Val 
        515                 520                 525             


Leu Tyr Val Val Lys Lys Gly Ser Ile Ser Phe Glu Asp Leu Lys Ala 
    530                 535                 540                 


Gln Ser Asn Glu Glu Val Val Gln Leu Ser Pro Asp Glu Glu Thr Lys 
545                 550                 555                 560 


Asp Leu Ile His Arg Leu Phe His Pro Gly Glu His Val Arg Asp Cys 
                565                 570                 575     


Leu Ser Asp Leu Leu Gly His Pro Phe Phe Trp Thr Trp Glu Ser Arg 
            580                 585                 590         


Tyr Arg Thr Leu Arg Asn Val Gly Asn Glu Ser Asp Ile Lys Thr Arg 
        595                 600                 605             


Lys Ser Glu Ser Glu Ile Leu Arg Leu Leu Gln Pro Gly Pro Ser Glu 
    610                 615                 620                 


His Ser Lys Ser Phe Asp Lys Trp Thr Thr Lys Met Ser Lys Leu Arg 
625                 630                 635                 640 


His Arg Gln Ile Ile Phe Pro Thr Thr Gln Asn Gln 
                645                 650         


<210>  3
<211>  19
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE

<400>  3
gccagatata cgcgttgac                                                    19


<210>  4
<211>  20
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE

<220>
<221>  misc_feature
<222>  (1)
<223>  n = mg

<220>
<221>  misc_feature
<222>  (2)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (3)
<223>  n = mu

<220>
<221>  misc_feature
<222>  (4)
<223>  n = mg

<220>
<221>  misc_feature
<222>  (5)
<223>  n = ma

<220>
<221>  misc_feature
<222>  (16)
<223>  n = mu

<220>
<221>  misc_feature
<222>  (17)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (18)
<223>  n = mu

<220>
<221>  misc_feature
<222>  (19)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (20)
<223>  n = ma

<400>  4
nnnnnccttt ttgctnnnnn                                          20


<210>  5
<211>  23
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE

<220>
<221>  misc_feature
<222>  (1)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (2)
<223>  n = ma

<220>
<221>  misc_feature
<222>  (3)
<223>  n = mu

<220>
<221>  misc_feature
<222>  (4)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (5)
<223>  n = mu

<220>
<221>  misc_feature
<222>  (19)
<223>  n = mg

<220>
<221>  misc_feature
<222>  (20)
<223>  n = mg

<220>
<221>  misc_feature
<222>  (21)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (22)
<223>  n = ma

<220>
<221>  misc_feature
<222>  (23)
<223>  n = mu

<400>  5
nnnnnccagc accgttaann nnn                                    23


<210>  6
<211>  20
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE

<400>  6
gctgaccttt ttgcttctca                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE

<220>
<221>  misc_feature
<222>  (1)
<223>  n = mg

<220>
<221>  misc_feature
<222>  (2)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (3)
<223>  n = mu

<220>
<221>  misc_feature
<222>  (4)
<223>  n = mg

<220>
<221>  misc_feature
<222>  (5)
<223>  n = ma

<220>
<221>  misc_feature
<222>  (16)
<223>  n = mu

<220>
<221>  misc_feature
<222>  (17)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (18)
<223>  n = mu

<220>
<221>  misc_feature
<222>  (19)
<223>  n = mc

<220>
<221>  misc_feature
<222>  (20)
<223>  n = ma

<400>  7
nnnnnccttt ttgctnnnnn                                         20


<210>  8
<211>  32
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE

<400>  8
acactctttc cctacacgac gctcttccga tc                                     32


<210>  9
<211>  23
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is gm, cm, um, or am

<220>
<221>  misc_feature
<222>  (6)..(18)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(23)
<223>  n is gm, cm, um, or am

<400>  9
nnnnnnnnnn nnnnnnnnnn nnn                                    23


<210>  10
<211>  10
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  n is a, c, g, or t

<400>  10
nnnnnnnnnn                                                              10


<210>  11
<211>  5
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  ANTISENSE OLIGONUCLEOTIDE


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is a, c, g, or t

<400>  11
nnnnn                                                                    5


<210>  12
<211>  23
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  MDA5 FORWARD PRIMER

<400>  12
gcttctagtt agagacgtct tgg                                               23


<210>  13
<211>  23
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  MDA5 REVERSE PRIMER

<400>  13
cttacacctg attcatttcc att                                               23


<210>  14
<211>  18
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  OASL FORWARD PRIMER

<400>  14
gagcagagag tccccgat                                                     18


<210>  15
<211>  20
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  OASL REVERSE PRIMER

<400>  15
ctgggagttg ggaagagaag                                                   20


<210>  16
<211>  22
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GAPDH FORWARD PRIMER

<400>  16
tgtagttgag gtcaatgaag gg                                                22


<210>  17
<211>  19
<212>  DNA
<213>  ARTIFICIAL SEQUENCE

<220>
<223>  GAPDH REVERSE PRIMER

<400>  17
acatcgctca gacaccatg                                                    19


