                         SEQUENCE LISTING

<110>  UNIVERSIDADE DE SANTIAGO DE COMPOSTELA
 
<120>  Mtodo de produccin de la protena glucosa-6-fosfatasa 2

<130>  P15641PC00

<150>  ES P201830351
<151>  2018-04-09

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  66
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragmento 477-542 de la protena muNS del Orthoreovirus aviar

<400>  1

Glu Asp His Leu Leu Ala Tyr Leu Asn Glu His Val Cys Val Asn Ala 
1               5                   10                  15      


Lys Asp His Glu Lys Gly Leu Leu Ala Arg Cys Asn Val Ser Gly Asp 
            20                  25                  30          


Ser Ile Ser Ser Ile Leu Gly Gln Arg Met Lys Asn Arg Glu Arg Phe 
        35                  40                  45              


Glu Thr Arg Leu Arg His Glu Ala Ser Ala Glu Trp Glu Pro Arg Val 
    50                  55                  60                  


Glu Ala 
65      


<210>  2
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragmento 448-635 de la protena muNS del Orthoreovirus aviar

<400>  2

Pro Ala Ala Leu Leu Ser Lys Ile Ala Asp Leu Gln Arg Ala Asn Arg 
1               5                   10                  15      


Glu Leu Ser Leu Lys Leu Val Asp Val Gln Pro Ala Arg Glu Asp His 
            20                  25                  30          


Leu Leu Ala Tyr Leu Asn Glu His Val Cys Val Asn Ala Lys Asp His 
        35                  40                  45              


Glu Lys Gly Leu Leu Ala Arg Cys Asn Val Ser Gly Asp Ser Ile Ser 
    50                  55                  60                  


Ser Ile Leu Gly Gln Arg Met Lys Asn Arg Glu Arg Phe Glu Thr Arg 
65                  70                  75                  80  


Leu Arg His Glu Ala Ser Ala Glu Trp Glu Pro Arg Val Glu Ala Leu 
                85                  90                  95      


Asn Gln Glu Leu Ala Lys Ala Arg Val Glu Gln Gln Asp Met Met Thr 
            100                 105                 110         


Gln Ser Leu Gln Tyr Leu Asn Glu Arg Asp Glu Leu Leu Gln Glu Val 
        115                 120                 125             


Asp Glu Leu Lys Arg Glu Leu Thr Thr Leu Arg Ser Ala Asn Val Arg 
    130                 135                 140                 


Leu Asn Ala Asp Asn His Arg Met Ser Arg Ala Thr Arg Val Gly Asp 
145                 150                 155                 160 


Ala Phe Val Ser Asp Val Glu Pro Leu Pro Ser Gly Ile Pro Gly Glu 
                165                 170                 175     


Ser Lys Pro Ser Met Glu Glu Leu Val Asp Asp Leu 
            180                 185             


<210>  3
<211>  635
<212>  PRT
<213>  Ortoreovirus aviar

<400>  3

Met Ala Ser Thr Lys Trp Gly Asp Lys Pro Met Ser Val Ser Met Ser 
1               5                   10                  15      


His Asp Gly Ser Ser Ile Arg Ser Ala Ala Ser Gln Phe Leu Ser Gly 
            20                  25                  30          


Pro Leu Phe His Ser Thr Pro Ile Pro Pro Gln Arg Lys Thr Val Leu 
        35                  40                  45              


Leu Lys Phe Met Ile Gly Asp Glu Leu Val Thr Val Gln Gly Ala Leu 
    50                  55                  60                  


Ala Pro Phe Asp Glu Tyr Trp Tyr Asp Asn Gln Pro Leu Leu Ala Gln 
65                  70                  75                  80  


Ala Val Glu Met Leu Ala Ser Ala Asp Arg Leu Arg Gln Phe Glu His 
                85                  90                  95      


Tyr Glu Lys Phe Leu Leu Lys Lys Gly His Gln Ile Thr Glu Ile Met 
            100                 105                 110         


Asn Arg Leu Arg Leu Phe Phe Thr Asp Val Leu Lys Val Lys Met Glu 
        115                 120                 125             


Ala Asp Ala Leu Pro Ala Leu Ala Gln Tyr Leu Met Val Gly Thr Leu 
    130                 135                 140                 


Glu Ala Val Ser Thr Ala Asp Ser Pro Asp Ala Cys Val Pro Val Thr 
145                 150                 155                 160 


Ser Lys Ile Leu Ala Lys Gln Gln Thr Ile Ala Lys Ser Pro Gly Arg 
                165                 170                 175     


Leu Asp Glu Glu Glu Tyr Asn Val Ile Arg Ser Arg Phe Leu Thr His 
            180                 185                 190         


Glu Val Phe Asp Leu Thr Ser Asp Leu Pro Gly Val Gln Pro Phe Met 
        195                 200                 205             


Asp Met Tyr Tyr Ala Thr Val Pro Arg Ala Asp Ser Thr Gly Trp Cys 
    210                 215                 220                 


Val Tyr Arg Arg Lys Gly Leu Leu Ile His Ala Pro Asp Glu Gln Phe 
225                 230                 235                 240 


Ser Asp Leu Thr Ile Phe Ser Thr Arg Leu Thr Ala Ser Arg Glu Leu 
                245                 250                 255     


Gln Leu Val Ala Gly Asp Val Ala Val Ala Cys Phe Asp Leu Met Asp 
            260                 265                 270         


Val Ser Asp Ile Ala Pro Ser His His Ala Ser Val Gln Glu Glu Arg 
        275                 280                 285             


Thr Leu Gly Thr Ser Arg Tyr Ser Asn Val Thr Ala Asn Asp His Pro 
    290                 295                 300                 


Leu Val Phe Phe Ser Pro Ser Ala Leu Arg Trp Ala Ile Asp His Ala 
305                 310                 315                 320 


Cys Thr Asp Ser Leu Val Ser Thr Arg Asn Ile Arg Val Cys Val Gly 
                325                 330                 335     


Ile Asp Pro Leu Val Thr Arg Trp Thr Arg Asp Gly Val Gln Glu Ala 
            340                 345                 350         


Ala Ile Leu Met Asp Asp Lys Leu Pro Ser Ala Gly Arg Ala Arg Met 
        355                 360                 365             


Ala Leu Arg Thr Leu Leu Leu Ala Arg Arg Ser Pro Met Thr Ser Phe 
    370                 375                 380                 


Leu Leu Gly Ala Leu Lys Gln Ser Gly Gly Gln Leu Met Glu His Tyr 
385                 390                 395                 400 


Arg Cys Asp Ala Ala Asn Arg Tyr Gly Ser Pro Thr Val Pro Val Ser 
                405                 410                 415     


His Ser Pro Pro Cys Ser Lys Cys Pro Glu Leu Lys Glu Gln Ile Thr 
            420                 425                 430         


Lys Leu Ser Ser Ser Pro Leu Pro Lys Ile Asp Ser Asn Val Gly Pro 
        435                 440                 445             


Ala Ala Leu Leu Ser Lys Ile Ala Asp Leu Gln Arg Ala Asn Arg Glu 
    450                 455                 460                 


Leu Ser Leu Lys Leu Val Asp Val Gln Pro Ala Arg Glu Asp His Leu 
465                 470                 475                 480 


Leu Ala Tyr Leu Asn Glu His Val Cys Val Asn Ala Lys Asp His Glu 
                485                 490                 495     


Lys Gly Leu Leu Ser Arg Cys Asn Val Ser Gly Asp Ser Ile Ser Ser 
            500                 505                 510         


Ile Leu Gly Gln Arg Val Lys Asn Arg Glu Arg Phe Glu Thr Arg Leu 
        515                 520                 525             


Arg His Glu Ala Ser Ala Glu Trp Glu Pro Arg Val Glu Ala Leu Asn 
    530                 535                 540                 


Gln Glu Leu Ala Lys Ala Arg Val Glu Gln Gln Asp Met Met Thr Gln 
545                 550                 555                 560 


Ser Leu Gln Tyr Leu Asn Glu Arg Asp Glu Leu Leu His Glu Val Asp 
                565                 570                 575     


Glu Leu Lys Arg Glu Leu Thr Thr Leu Arg Ser Ala Asn Val Arg Leu 
            580                 585                 590         


Asn Ala Asp Asn His Arg Met Ser Arg Ala Thr Arg Val Gly Asp Ala 
        595                 600                 605             


Phe Val Ser Asp Val Glu Pro Leu Pro Ser Gly Ile Pro Gly Glu Ser 
    610                 615                 620                 


Lys Pro Ser Met Glu Glu Leu Val Asp Asp Leu 
625                 630                 635 


<210>  4
<211>  721
<212>  PRT
<213>  Ortoreovirus de mamfero

<400>  4

Met Ala Ser Phe Lys Gly Phe Ser Ala Asn Thr Val Pro Val Ser Lys 
1               5                   10                  15      


Ala Lys Arg Asp Ile Ser Ser Leu Ala Ala Thr Pro Gly Leu Arg Ser 
            20                  25                  30          


Gln Ser Phe Thr Pro Ser Val Asp Met Ser Gln Ser Arg Glu Phe Leu 
        35                  40                  45              


Thr Lys Ala Ile Glu Gln Gly Ser Met Ser Ile Pro Tyr Gln His Val 
    50                  55                  60                  


Asn Val Pro Lys Val Asp Arg Lys Val Val Ser Leu Val Val Arg Pro 
65                  70                  75                  80  


Phe Ser Ser Gly Ala Phe Ser Ile Ser Gly Val Ile Ser Pro Ala His 
                85                  90                  95      


Ala Tyr Leu Leu Glu Cys Leu Pro Gln Leu Glu Gln Ala Met Ala Phe 
            100                 105                 110         


Val Ala Ser Pro Glu Ser Phe Gln Ala Ser Asp Val Ala Lys Arg Phe 
        115                 120                 125             


Ala Ile Lys Pro Gly Met Ser Leu Gln Asp Ala Ile Thr Ala Phe Ile 
    130                 135                 140                 


Asn Phe Val Ser Ala Met Leu Lys Met Thr Val Thr Arg Gln Asn Phe 
145                 150                 155                 160 


Asp Val Ile Val Ala Glu Ile Glu Arg Leu Ala Ser Thr Ser Val Ser 
                165                 170                 175     


Val Arg Thr Lys Glu Ala Lys Val Ala Asp Glu Glu Leu Met Leu Phe 
            180                 185                 190         


Gly Leu Asp His Arg Gly Pro Gln Gln Leu Asp Val Ser Asp Ala Lys 
        195                 200                 205             


Gly Ile Met Lys Ala Ala Asp Ile Gln Thr Thr His Asp Val His Leu 
    210                 215                 220                 


Ala Pro Gly Val Gly Asn Ile Asp Pro Glu Ile Tyr Asn Glu Gly Arg 
225                 230                 235                 240 


Phe Met Phe Met Gln His Lys Pro Leu Ala Ala Asp Gln Ser Tyr Phe 
                245                 250                 255     


Thr Leu Glu Thr Ala Asp Tyr Phe Lys Ile Tyr Pro Thr Tyr Asp Glu 
            260                 265                 270         


His Asp Gly Arg Met Ala Asp Gln Lys Gln Ser Gly Leu Ile Leu Cys 
        275                 280                 285             


Thr Lys Asp Glu Val Leu Ala Glu Gln Thr Ile Phe Lys Leu Asp Ala 
    290                 295                 300                 


Pro Asp Asp Lys Thr Val His Leu Leu Asp Arg Asp Asp Asp His Val 
305                 310                 315                 320 


Val Ala Arg Phe Thr Lys Val Phe Ile Glu Asp Val Ala Pro Gly His 
                325                 330                 335     


His Ala Ala Gln Arg Ser Gly Gln Arg Ser Val Leu Asp Asp Leu Tyr 
            340                 345                 350         


Ala Asn Thr Gln Val Ile Ser Ile Thr Ser Ala Ala Leu Lys Trp Val 
        355                 360                 365             


Val Lys His Gly Val Ser Asp Gly Ile Val Asn Arg Lys Asn Val Lys 
    370                 375                 380                 


Val Cys Val Gly Phe Asp Pro Leu Tyr Thr Leu Ser Thr His Asn Gly 
385                 390                 395                 400 


Val Ser Leu Cys Ala Leu Leu Met Asp Glu Lys Leu Ser Val Leu Asn 
                405                 410                 415     


Ser Ala Cys Arg Met Thr Leu Arg Ser Leu Met Lys Thr Gly Arg Asp 
            420                 425                 430         


Val Asp Ala His Arg Ala Phe Gln Arg Val Leu Ser Gln Gly Tyr Thr 
        435                 440                 445             


Ser Leu Met Cys Tyr Tyr His Pro Ser Arg Lys Leu Ala Tyr Gly Glu 
    450                 455                 460                 


Val Leu Phe Leu Glu Arg Ser Asn Asp Val Thr Asp Gly Ile Lys Leu 
465                 470                 475                 480 


Gln Leu Asp Ala Ser Arg Gln Cys His Glu Cys Pro Val Leu Gln Gln 
                485                 490                 495     


Lys Val Val Glu Leu Glu Lys Gln Ile Ile Met Gln Lys Ser Ile Gln 
            500                 505                 510         


Ser Asp Pro Thr Pro Val Ala Leu Gln Pro Leu Leu Ser Gln Leu Arg 
        515                 520                 525             


Glu Leu Ser Ser Glu Val Thr Arg Leu Gln Met Glu Leu Ser Arg Ala 
    530                 535                 540                 


Gln Ser Leu Asn Ala Gln Leu Glu Ala Asp Val Lys Ser Ala Gln Ser 
545                 550                 555                 560 


Cys Ser Leu Asp Met Tyr Leu Arg His His Thr Cys Ile Asn Gly His 
                565                 570                 575     


Ala Lys Glu Asp Glu Leu Leu Asp Ala Val Arg Val Ala Pro Asp Val 
            580                 585                 590         


Arg Arg Glu Ile Met Glu Lys Arg Ser Glu Val Arg Gln Gly Trp Cys 
        595                 600                 605             


Glu Arg Ile Ser Lys Glu Ala Ala Ala Lys Cys Gln Thr Val Ile Asp 
    610                 615                 620                 


Asp Leu Thr Leu Met Asn Gly Lys Gln Ala Gln Glu Ile Thr Glu Leu 
625                 630                 635                 640 


Arg Asp Ser Ala Glu Lys Tyr Glu Lys Gln Ile Ala Glu Leu Val Ser 
                645                 650                 655     


Thr Ile Thr Gln Asn Gln Ile Thr Tyr Gln Gln Glu Leu Gln Ala Leu 
            660                 665                 670         


Val Ala Lys Asn Val Glu Leu Asp Ala Leu Asn Gln Arg Gln Ala Lys 
        675                 680                 685             


Ser Leu Arg Ile Thr Pro Ser Leu Leu Ser Ala Thr Pro Ile Asp Ser 
    690                 695                 700                 


Ala Asp Gly Val Ala Asp Leu Ile Asp Phe Ser Val Pro Thr Asp Glu 
705                 710                 715                 720 


Leu 
    


<210>  5
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragmento 561-622 de la protena muNS de Orthoreovirus de 
       mamfero

<400>  5

Cys Ser Leu Asp Met Tyr Leu Arg His His Thr Cys Ile Asn Gly His 
1               5                   10                  15      


Ala Lys Glu Asp Glu Leu Leu Asp Ala Val Arg Val Ala Pro Asp Val 
            20                  25                  30          


Arg Arg Glu Ile Met Glu Lys Arg Ser Glu Val Arg Gln Gly Trp Cys 
        35                  40                  45              


Glu Arg Ile Ser Lys Glu Ala Ala Ala Lys Cys Gln Thr Val 
    50                  55                  60          


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sitio de corte de enteroquinasa

<400>  6

Asp Asp Asp Asp Lys 
1               5   


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sitio de corte de factor Xa

<400>  7

Ile Glu Asp Gly Arg 
1               5   


<210>  8
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sitio de corte de trombina

<400>  8

Leu Val Pro Arg Gly Ser 
1               5       


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sitio de corte de proteasa TEV

<400>  9

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sitio de corte de proteasa PreScission

<400>  10

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  11
<211>  204
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fragmento 518 -721 de la protena muNS de Orthoreovirus de 
       mamfero .

<400>  11

Val Ala Leu Gln Pro Leu Leu Ser Gln Leu Arg Glu Leu Ser Ser Glu 
1               5                   10                  15      


Val Thr Arg Leu Gln Met Glu Leu Ser Arg Ala Gln Ser Leu Asn Ala 
            20                  25                  30          


Gln Leu Glu Ala Asp Val Lys Ser Ala Gln Ser Cys Ser Leu Asp Met 
        35                  40                  45              


Tyr Leu Arg His His Thr Cys Ile Asn Gly His Ala Lys Glu Asp Glu 
    50                  55                  60                  


Leu Leu Asp Ala Val Arg Val Ala Pro Asp Val Arg Arg Glu Ile Met 
65                  70                  75                  80  


Glu Lys Arg Ser Glu Val Arg Gln Gly Trp Cys Glu Arg Ile Ser Lys 
                85                  90                  95      


Glu Ala Ala Ala Lys Cys Gln Thr Val Ile Asp Asp Leu Thr Leu Met 
            100                 105                 110         


Asn Gly Lys Gln Ala Gln Glu Ile Thr Glu Leu Arg Asp Ser Ala Glu 
        115                 120                 125             


Lys Tyr Glu Lys Gln Ile Ala Glu Leu Val Ser Thr Ile Thr Gln Asn 
    130                 135                 140                 


Gln Ile Thr Tyr Gln Gln Glu Leu Gln Ala Leu Val Ala Lys Asn Val 
145                 150                 155                 160 


Glu Leu Asp Ala Leu Asn Gln Arg Gln Ala Lys Ser Leu Arg Ile Thr 
                165                 170                 175     


Pro Ser Leu Leu Ser Ala Thr Pro Ile Asp Ser Ala Asp Gly Val Ala 
            180                 185                 190         


Asp Leu Ile Asp Phe Ser Val Pro Thr Asp Glu Leu 
        195                 200                 


<210>  12
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador sentido IGRP

<400>  12
gcgaagcttg gcatggattt ccttcacagg aatgg                                  35


<210>  13
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador antisentido IGRP

<400>  13
gcgaagcttc tactgactct tctttccgct ttgtttc                                37


<210>  14
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador sentido IC

<400>  14
gcataagaat gcggccgcta tcatggcgga agatcacttg ttggcttatc                  50


<210>  15
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador antisentido IC

<400>  15
gcgtctagac gcttccacac ggggttccca ctcag                                  35


<210>  16
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador sentido muNS-Mi

<400>  16
catgccatgg caccagccgt actgctgtc                                         29


<210>  17
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador antisentido muNS-Mi

<400>  17
ttgcggccgc aatcacagat catccacc                                          28


<210>  18
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador sentido IC-IGRP

<400>  18
ggagatctcg cggaagatca cttgttggc                                         29


<210>  19
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cebador antisentido IC-IGRP

<400>  19
gggatatcct actgactctt ctttccgc                                          28


