
                                SEQUENCE LISTING

<110> JUNO THERAPEUTICS, INC.
      EDITAS MEDICINE, INC.
      SATHER, Blythe D.
      BORGES, Christopher
      BURLEIGH, Stephen Michael
      NYE, Christopher Heath
      VONG, Queenie
      WELSTEAD, G. Grant

<120> METHODS OF PRODUCING CELLS EXPRESSING A
  RECOMBINANT RECEPTOR AND RELATED COMPOSITIONS
  

<130> 735042012740

<140> Not Yet Assigned    
<141> Concurrently Herewith  

<150> 62/653,522          
<151> 2018-04-05  

<160> 256

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 4627
<212> DNA
<213> Homo sapiens

<220> 
<223> Human TCR alpha constant (TRAC)

<300> 
<308> NG_001332.3
<309> 2019-02-26  

<400> 1
atatccagaa ccctgaccct gccgtgtacc agctgagaga ctctaaatcc agtgacaagt 60
ctgtctgcct attcaccgat tttgattctc aaacaaatgt gtcacaaagt aaggattctg 120
atgtgtatat cacagacaaa actgtgctag acatgaggtc tatggacttc aagagcaaca 180
gtgctgtggc ctggagcaac aaatctgact ttgcatgtgc aaacgccttc aacaacagca 240
ttattccaga agacaccttc ttccccagcc caggtaaggg cagctttggt gccttcgcag 300
gctgtttcct tgcttcagga atggccaggt tctgcccaga gctctggtca atgatgtcta 360
aaactcctct gattggtggt ctcggcctta tccattgcca ccaaaaccct ctttttacta 420
agaaacagtg agccttgttc tggcagtcca gagaatgaca cgggaaaaaa gcagatgaag 480
agaaggtggc aggagagggc acgtggccca gcctcagtct ctccaactga gttcctgcct 540
gcctgccttt gctcagactg tttgcccctt actgctcttc taggcctcat tctaagcccc 600
ttctccaagt tgcctctcct tatttctccc tgtctgccaa aaaatctttc ccagctcact 660
aagtcagtct cacgcagtca ctcattaacc caccaatcac tgattgtgcc ggcacatgaa 720
tgcaccaggt gttgaagtgg aggaattaaa aagtcagatg aggggtgtgc ccagaggaag 780
caccattcta gttgggggag cccatctgtc agctgggaaa agtccaaata acttcagatt 840
ggaatgtgtt ttaactcagg gttgagaaaa cagctacctt caggacaaaa gtcagggaag 900
ggctctctga agaaatgcta cttgaagata ccagccctac caagggcagg gagaggaccc 960
tatagaggcc tgggacagga gctcaatgag aaaggagaag agcagcaggc atgagttgaa 1020
tgaaggaggc agggccgggt cacagggcct tctaggccat gagagggtag acagtattct 1080
aaggacgcca gaaagctgtt gatcggcttc aagcagggga gggacaccta atttgctttt 1140
cttttttttt tttttttttt tttttttttt tgagatggag ttttgctctt gttgcccagg 1200
ctggagtgca atggtgcatc ttggctcact gcaacctccg cctcccaggt tcaagtgatt 1260
ctcctgcctc agcctcccga gtagctgaga ttacaggcac ccgccaccat gcctggctaa 1320
ttttttgtat ttttagtaga gacagggttt cactatgttg gccaggctgg tctcgaactc 1380
ctgacctcag gtgatccacc cgcttcagcc tcccaaagtg ctgggattac aggcgtgagc 1440
caccacaccc ggcctgcttt tcttaaagat caatctgagt gctgtacgga gagtgggttg 1500
taagccaaga gtagaagcag aaagggagca gttgcagcag agagatgatg gaggcctggg 1560
cagggtggtg gcagggaggt aaccaacacc attcaggttt caaaggtaga accatgcagg 1620
gatgagaaag caaagagggg atcaaggaag gcagctggat tttggcctga gcagctgagt 1680
caatgatagt gccgtttact aagaagaaac caaggaaaaa atttggggtg cagggatcaa 1740
aactttttgg aacatatgaa agtacgtgtt tatactcttt atggcccttg tcactatgta 1800
tgcctcgctg cctccattgg actctagaat gaagccaggc aagagcaggg tctatgtgtg 1860
atggcacatg tggccagggt catgcaacat gtactttgta caaacagtgt atattgagta 1920
aatagaaatg gtgtccagga gccgaggtat cggtcctgcc agggccaggg gctctcccta 1980
gcaggtgctc atatgctgta agttccctcc agatctctcc acaaggaggc atggaaaggc 2040
tgtagttgtt cacctgccca agaactagga ggtctggggt gggagagtca gcctgctctg 2100
gatgctgaaa gaatgtctgt ttttcctttt agaaagttcc tgtgatgtca agctggtcga 2160
gaaaagcttt gaaacaggta agacaggggt ctagcctggg tttgcacagg attgcggaag 2220
tgatgaaccc gcaataaccc tgcctggatg agggagtggg aagaaattag tagatgtggg 2280
aatgaatgat gaggaatgga aacagcggtt caagacctgc ccagagctgg gtggggtctc 2340
tcctgaatcc ctctcaccat ctctgacttt ccattctaag cactttgagg atgagtttct 2400
agcttcaata gaccaaggac tctctcctag gcctctgtat tcctttcaac agctccactg 2460
tcaagagagc cagagagagc ttctgggtgg cccagctgtg aaatttctga gtcccttagg 2520
gatagcccta aacgaaccag atcatcctga ggacagccaa gaggttttgc cttctttcaa 2580
gacaagcaac agtactcaca taggctgtgg gcaatggtcc tgtctctcaa gaatcccctg 2640
ccactcctca cacccaccct gggcccatat tcatttccat ttgagttgtt cttattgagt 2700
catccttcct gtggtagcgg aactcactaa ggggcccatc tggacccgag gtattgtgat 2760
gataaattct gagcacctac cccatcccca gaagggctca gaaataaaat aagagccaag 2820
tctagtcggt gtttcctgtc ttgaaacaca atactgttgg ccctggaaga atgcacagaa 2880
tctgtttgta aggggatatg cacagaagct gcaagggaca ggaggtgcag gagctgcagg 2940
cctcccccac ccagcctgct ctgccttggg gaaaaccgtg ggtgtgtcct gcaggccatg 3000
caggcctggg acatgcaagc ccataaccgc tgtggcctct tggttttaca gatacgaacc 3060
taaactttca aaacctgtca gtgattgggt tccgaatcct cctcctgaaa gtggccgggt 3120
ttaatctgct catgacgctg cggctgtggt ccagctgagg tgaggggcct tgaagctggg 3180
agtggggttt agggacgcgg gtctctgggt gcatcctaag ctctgagagc aaacctccct 3240
gcagggtctt gcttttaagt ccaaagcctg agcccaccaa actctcctac ttcttcctgt 3300
tacaaattcc tcttgtgcaa taataatggc ctgaaacgct gtaaaatatc ctcatttcag 3360
ccgcctcagt tgcacttctc ccctatgagg taggaagaac agttgtttag aaacgaagaa 3420
actgaggccc cacagctaat gagtggagga agagagacac ttgtgtacac cacatgcctt 3480
gtgttgtact tctctcaccg tgtaacctcc tcatgtcctc tctccccagt acggctctct 3540
tagctcagta gaaagaagac attacactca tattacaccc caatcctggc tagagtctcc 3600
gcaccctcct cccccagggt ccccagtcgt cttgctgaca actgcatcct gttccatcac 3660
catcaaaaaa aaactccagg ctgggtgcgg gggctcacac ctgtaatccc agcactttgg 3720
gaggcagagg caggaggagc acaggagctg gagaccagcc tgggcaacac agggagaccc 3780
cgcctctaca aaaagtgaaa aaattaacca ggtgtggtgc tgcacacctg tagtcccagc 3840
tacttaagag gctgagatgg gaggatcgct tgagccctgg aatgttgagg ctacaatgag 3900
ctgtgattgc gtcactgcac tccagcctgg aagacaaagc aagatcctgt ctcaaataat 3960
aaaaaaaata agaactccag ggtacatttg ctcctagaac tctaccacat agccccaaac 4020
agagccatca ccatcacatc cctaacagtc ctgggtcttc ctcagtgtcc agcctgactt 4080
ctgttcttcc tcattccaga tctgcaagat tgtaagacag cctgtgctcc ctcgctcctt 4140
cctctgcatt gcccctcttc tccctctcca aacagaggga actctcctac ccccaaggag 4200
gtgaaagctg ctaccacctc tgtgcccccc cggcaatgcc accaactgga tcctacccga 4260
atttatgatt aagattgctg aagagctgcc aaacactgct gccaccccct ctgttccctt 4320
attgctgctt gtcactgcct gacattcacg gcagaggcaa ggctgctgca gcctcccctg 4380
gctgtgcaca ttccctcctg ctccccagag actgcctccg ccatcccaca gatgatggat 4440
cttcagtggg ttctcttggg ctctaggtcc tgcagaatgt tgtgaggggt ttattttttt 4500
ttaatagtgt tcataaagaa atacatagta ttcttcttct caagacgtgg ggggaaatta 4560
tctcattatc gaggccctgc tatgctgtgt atctgggcgt gttgtatgtc ctgctgccga 4620
tgccttc                                                           4627

<210> 2
<211> 1448
<212> DNA
<213> Homo sapiens

<220> 
<223> Human TCR beta constant 1 (TRBC1)
      

<300> 
<308> NG_001333.2
<309> 2018-09-23  

<400> 2
aggacctgaa caaggtgttc ccacccgagg tcgctgtgtt tgagccatca gaagcagaga 60
tctcccacac ccaaaaggcc acactggtgt gcctggccac aggcttcttc cccgaccacg 120
tggagctgag ctggtgggtg aatgggaagg aggtgcacag tggggtcagc acagacccgc 180
agcccctcaa ggagcagccc gccctcaatg actccagata ctgcctgagc agccgcctga 240
gggtctcggc caccttctgg cagaaccccc gcaaccactt ccgctgtcaa gtccagttct 300
acgggctctc ggagaatgac gagtggaccc aggatagggc caaacccgtc acccagatcg 360
tcagcgccga ggcctggggt agagcaggtg agtggggcct ggggagatgc ctggaggaga 420
ttaggtgaga ccagctacca gggaaaatgg aaagatccag gtagcagaca agactagatc 480
caaaaagaaa ggaaccagcg cacaccatga aggagaattg ggcacctgtg gttcattctt 540
ctcccagatt ctcagcccaa cagagccaag cagctgggtc ccctttctat gtggcctgtg 600
taactctcat ctgggtggtg ccccccatcc ccctcagtgc tgccacatgc catggattgc 660
aaggacaatg tggctgacat ctgcatggca gaagaaagga ggtgctgggc tgtcagagga 720
agctggtctg ggcctgggag tctgtgccaa ctgcaaatct gactttactt ttaattgcct 780
atgaaaataa ggtctctcat ttattttcct ctccctgctt tctttcagac tgtggcttta 840
cctcgggtaa gtaagccctt ccttttcctc tccctctctc atggttcttg acctagaacc 900
aaggcatgaa gaactcacag acactggagg gtggagggtg ggagagacca gagctacctg 960
tgcacaggta cccacctgtc cttcctccgt gccaacagtg tcctaccagc aaggggtcct 1020
gtctgccacc atcctctatg agatcctgct agggaaggcc accctgtatg ctgtgctggt 1080
cagcgccctt gtgttgatgg ccatggtaag caggagggca ggatggggcc agcaggctgg 1140
aggtgacaca ctgacaccaa gcacccagaa gtatagagtc cctgccagga ttggagctgg 1200
gcagtaggga gggaagagat ttcattcagg tgcctcagaa gataacttgc acctctgtag 1260
gatcacagtg gaagggtcat gctgggaagg agaagctgga gtcaccagaa aacccaatgg 1320
atgttgtgat gagccttact atttgtgtgg tcaatgggcc ctactacttt ctctcaatcc 1380
tcacaactcc tggctcttaa taacccccaa aactttctct tctgcaggtc aagagaaagg 1440
atttctga                                                          1448

<210> 3
<211> 1489
<212> DNA
<213> Homo sapiens

<220> 
<223> Human TCR beta constant 2 (TRBC2)

<300> 
<308> NG_001333.2
<309> 2018-09-23  

<400> 3
aggacctgaa aaacgtgttc ccacccgagg tcgctgtgtt tgagccatca gaagcagaga 60
tctcccacac ccaaaaggcc acactggtat gcctggccac aggcttctac cccgaccacg 120
tggagctgag ctggtgggtg aatgggaagg aggtgcacag tggggtcagc acagacccgc 180
agcccctcaa ggagcagccc gccctcaatg actccagata ctgcctgagc agccgcctga 240
gggtctcggc caccttctgg cagaaccccc gcaaccactt ccgctgtcaa gtccagttct 300
acgggctctc ggagaatgac gagtggaccc aggatagggc caaacccgtc acccagatcg 360
tcagcgccga ggcctggggt agagcaggtg agtggggcct ggggagatgc ctggaggaga 420
ttaggtgaga ccagctacca gggaaaatgg aaagatccag gtagcggaca agactagatc 480
cagaagaaag ccagagtgga caaggtggga tgatcaaggt tcacagggtc agcaaagcac 540
ggtgtgcact tcccccacca agaagcatag aggctgaatg gagcacctca agctcattct 600
tccttcagat cctgacacct tagagctaag ctttcaagtc tccctgagga ccagccatac 660
agctcagcat ctgagtggtg tgcatcccat tctcttctgg ggtcctggtt tcctaagatc 720
atagtgacca cttcgctggc actggagcag catgagggag acagaaccag ggctatcaaa 780
ggaggctgac tttgtactat ctgatatgca tgtgtttgtg gcctgtgagt ctgtgatgta 840
aggctcaatg tccttacaaa gcagcattct ctcatccatt tttcttcccc tgttttcttt 900
cagactgtgg cttcacctcc ggtaagtgag tctctccttt ttctctctat ctttcgccgt 960
ctctgctctc gaaccagggc atggagaatc cacggacaca ggggcgtgag ggaggccaga 1020
gccacctgtg cacaggtgcc tacatgctct gttcttgtca acagagtctt accagcaagg 1080
ggtcctgtct gccaccatcc tctatgagat cttgctaggg aaggccacct tgtatgccgt 1140
gctggtcagt gccctcgtgc tgatggccat ggtaaggagg agggtgggat agggcagatg 1200
atgggggcag gggatggaac atcacacatg ggcataaagg aatctcagag ccagagcaca 1260
gcctaatata tcctatcacc tcaatgaaac cataatgaag ccagactggg gagaaaatgc 1320
agggaatatc acagaatgca tcatgggagg atggagacaa ccagcgagcc ctactcaaat 1380
taggcctcag agcccgcctc ccctgcccta ctcctgctgt gccatagccc ctgaaaccct 1440
gaaaatgttc tctcttccac aggtcaagag aaaggattcc agaggctag             1489

<210> 4
<211> 1189
<212> DNA
<213> Artificial Sequence

<220> 
<223> EF1alpha promoter

<300> 
<308> GenBank: J04617.1
<309> 1994-11-07  

<400> 4
cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 60
tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg 120
aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 180
gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa 240
gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt 300
gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 360
agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 420
gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 480
ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt 540
tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 600
tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 660
ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 720
ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 780
tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 840
aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 900
gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 960
cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 1020
tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 1080
ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 1140
cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaa             1189

<210> 5
<211> 1205
<212> DNA
<213> Artificial Sequence

<220> 
<223> EF1alpha promoter

<400> 5
cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt 60
tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg 120
aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa 180
gtgcactagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa 240
gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt 300
gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg 360
ggtgggagag ttcgtggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgtgg 420
cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg 480
ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt 540
tctggcaaga tagtcttgta aatgcgggcc aagatcagca cactggtatt tcggtttttg 600
gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc 660
tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctgcccgg cctgctctgg 720
tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg 780
caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agcacaaaat 840
ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct 900
ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc 960
tcgattagtt ctccagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg 1020
cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga 1080
tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc 1140
agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaaaacta cccctaaaag 1200
ccaaa                                                             1205

<210> 6
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> T2A

<400> 6
Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp
 1               5                  10                  15      
Val Glu Glu Asn Pro Gly Pro Arg
            20                  


<210> 7
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> T2A

<400> 7
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro
 1               5                  10                  15      
Gly Pro
        


<210> 8
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> P2A

<400> 8
Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val
 1               5                  10                  15      
Glu Glu Asn Pro Gly Pro
            20          


<210> 9
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> P2A

<400> 9
Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn
 1               5                  10                  15      
Pro Gly Pro
            


<210> 10
<211> 20
<212> PRT
<213> Artificial Sequence

<220> 
<223> E2A

<400> 10
Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser
 1               5                  10                  15      
Asn Pro Gly Pro
            20  


<210> 11
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> F2A

<400> 11
Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val
 1               5                  10                  15      
Glu Ser Asn Pro Gly Pro
            20          


<210> 12
<211> 357
<212> PRT
<213> Artificial Sequence

<220> 
<223> EGFRt

<400> 12
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly Ile Gly Ile Gly
            20                  25                  30          
Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn Ile Lys His Phe
        35                  40                  45              
Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile Leu Pro Val Ala
    50                  55                  60                  
Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu Asp Pro Gln Glu
65                  70                  75                  80  
Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly Phe Leu Leu Ile
                85                  90                  95      
Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala Phe Glu Asn Leu
            100                 105                 110         
Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln Phe Ser Leu Ala
        115                 120                 125             
Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu
    130                 135                 140                 
Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr
145                 150                 155                 160 
Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys
                165                 170                 175     
Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly
            180                 185                 190         
Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu
        195                 200                 205             
Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg Gly Arg Glu Cys
    210                 215                 220                 
Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg Glu Phe Val Glu
225                 230                 235                 240 
Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu Pro Gln Ala Met
                245                 250                 255     
Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala
            260                 265                 270         
His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys Pro Ala Gly Val
        275                 280                 285             
Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala Asp Ala Gly His
    290                 295                 300                 
Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro
305                 310                 315                 320 
Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala
                325                 330                 335     
Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val Val Ala Leu Gly
            340                 345                 350         
Ile Gly Leu Phe Met
        355         


<210> 13
<211> 335
<212> PRT
<213> Artificial Sequence

<220> 
<223> EGFRt

<400> 13
Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu
 1               5                  10                  15      
Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile
            20                  25                  30          
Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe
        35                  40                  45              
Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr
    50                  55                  60                  
Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn
65                  70                  75                  80  
Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg
                85                  90                  95      
Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile
            100                 105                 110         
Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val
        115                 120                 125             
Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp
    130                 135                 140                 
Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn
145                 150                 155                 160 
Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu
                165                 170                 175     
Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser
            180                 185                 190         
Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu
        195                 200                 205             
Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln
    210                 215                 220                 
Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly
225                 230                 235                 240 
Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro
                245                 250                 255     
His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr
            260                 265                 270         
Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His
        275                 280                 285             
Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro
    290                 295                 300                 
Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala
305                 310                 315                 320 
Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met
                325                 330                 335 


<210> 14
<211> 137
<212> PRT
<213> Mus musculus

<220> 
<223> Mouse TCR alpha constant

<400> 14
Asp Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg
 1               5                  10                  15      
Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile
            20                  25                  30          
Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Thr
        35                  40                  45              
Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala
    50                  55                  60                  
Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr
65                  70                  75                  80  
Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr
                85                  90                  95      
Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser
            100                 105                 110         
Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu
        115                 120                 125             
Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135         


<210> 15
<211> 137
<212> PRT
<213> Mus musculus

<220> 
<223> Mouse TCR alpha constant

<400> 15
Asn Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg
 1               5                  10                  15      
Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile
            20                  25                  30          
Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Thr
        35                  40                  45              
Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala
    50                  55                  60                  
Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr
65                  70                  75                  80  
Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr
                85                  90                  95      
Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser
            100                 105                 110         
Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu
        115                 120                 125             
Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135         


<210> 16
<211> 173
<212> PRT
<213> Mus musculus

<220> 
<223> Mouse TCR beta constant

<300> 
<308> Uniprot P01852
<309> 1986-07-21  

<400> 16
Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser Leu Phe Glu Pro
 1               5                  10                  15      
Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu Val Cys Leu
            20                  25                  30          
Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Ala Tyr Lys
    50                  55                  60                  
Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala
65                  70                  75                  80  
Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe
                85                  90                  95      
His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser Pro Lys Pro
            100                 105                 110         
Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly
        115                 120                 125             
Ile Thr Ser Ala Ser Tyr Gln Gln Gly Val Leu Ser Ala Thr Ile Leu
    130                 135                 140                 
Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val Ser
145                 150                 155                 160 
Thr Leu Val Val Met Ala Met Val Lys Arg Lys Asn Ser
                165                 170             


<210> 17
<211> 172
<212> PRT
<213> Mus musculus

<220> 
<223> Mouse TCR beta constant

<400> 17
Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser Leu Phe Glu Pro Ser
 1               5                  10                  15      
Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu Val Cys Leu Ala
            20                  25                  30          
Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn Gly
        35                  40                  45              
Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Ala Tyr Lys Glu
    50                  55                  60                  
Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala Thr
65                  70                  75                  80  
Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe His
                85                  90                  95      
Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser Pro Lys Pro Val
            100                 105                 110         
Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly Ile
        115                 120                 125             
Thr Ser Ala Ser Tyr His Gln Gly Val Leu Ser Ala Thr Ile Leu Tyr
    130                 135                 140                 
Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val Ser Gly
145                 150                 155                 160 
Leu Val Leu Met Ala Met Val Lys Arg Lys Asn Ser
                165                 170         


<210> 18
<211> 181
<212> DNA
<213> Artificial Sequence

<220> 
<223> MND promoter

<400> 18
gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 60
ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 120
tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 180
a                                                                 181

<210> 19
<211> 142
<212> PRT
<213> Homo sapiens

<220> 
<223> Human TCR alpha constant

<300> 
<308> Uniprot P01848
<309> 2018-07-18  

<400> 19
Pro Asn Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser
 1               5                  10                  15      
Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln
            20                  25                  30          
Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys
        35                  40                  45              
Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val
    50                  55                  60                  
Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn
65                  70                  75                  80  
Ser Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys
                85                  90                  95      
Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn
            100                 105                 110         
Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val
        115                 120                 125             
Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140         


<210> 20
<211> 177
<212> PRT
<213> Homo sapiens

<220> 
<223> Human TCR beta constant 1

<300> 
<308> Uniprot P01850
<309> 2018-07-18  

<400> 20
Glu Asp Leu Asn Lys Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro
 1               5                  10                  15      
Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu
            20                  25                  30          
Ala Thr Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys
    50                  55                  60                  
Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu
65                  70                  75                  80  
Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys
                85                  90                  95      
Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp
            100                 105                 110         
Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg
        115                 120                 125             
Ala Asp Cys Gly Phe Thr Ser Val Ser Tyr Gln Gln Gly Val Leu Ser
    130                 135                 140                 
Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala
145                 150                 155                 160 
Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp
                165                 170                 175     
Phe
    


<210> 21
<211> 178
<212> PRT
<213> Homo sapiens

<220> 
<223> Human TCR beta constant 2

<300> 
<308> Uniprot A0A5B9
<309> 2018-07-18  

<400> 21
Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro Ser
 1               5                  10                  15      
Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu Ala
            20                  25                  30          
Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn Gly
        35                  40                  45              
Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys Glu
    50                  55                  60                  
Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu Arg
65                  70                  75                  80  
Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys Gln
                85                  90                  95      
Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp Arg
            100                 105                 110         
Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg Ala
        115                 120                 125             
Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser Ala
    130                 135                 140                 
Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val
145                 150                 155                 160 
Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp Ser
                165                 170                 175     
Arg Gly
        


<210> 22
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker

<220> 
<221> REPEAT         
<222> (0)...(0)
<223> SGGGG is repeated 5 or 6 times

<400> 22
Pro Gly Gly Gly Ser Gly Gly Gly Gly Pro
 1               5                  10  


<210> 23
<211> 17
<212> PRT
<213> Artificial Sequence

<220> 
<223> Linker

<400> 23
Gly Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala Lys Lys Asp Gly Lys
 1               5                  10                  15      
Ser
    


<210> 24
<211> 141
<212> PRT
<213> Homo sapiens

<220> 
<223> human TCR alpha constant

<300> 
<308> CAA26636.1
<309> 2016-07-25  

<400> 24
Asn Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 25
<211> 179
<212> PRT
<213> Homo sapiens

<220> 
<223> human TCR beta constant

<300> 
<308> A0A0G2JNG9

<400> 25
Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro
 1               5                  10                  15      
Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu
            20                  25                  30          
Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys
    50                  55                  60                  
Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu
65                  70                  75                  80  
Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys
                85                  90                  95      
Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp
            100                 105                 110         
Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg
        115                 120                 125             
Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser
    130                 135                 140                 
Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala
145                 150                 155                 160 
Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp
                165                 170                 175     
Ser Arg Gly
            


<210> 26
<211> 149
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC gRNA transcription sequence

<400> 26
agcgctctcg tacagagttg gcattataat acgactcact ataggggaga atcaaaatcg 60
gtgaatgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga 120
aaaagtggca ccgagtcggt gcttttttt                                   149

<210> 27
<211> 100
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC gRNA sequence

<400> 27
gagaaucaaa aucggugaau guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                       100

<210> 28
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-10 gRNA targeting domain

<400> 28
ucucucagcu gguacacggc                                             20

<210> 29
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-110 gRNA targeting domain

<400> 29
uggauuuaga gucucucagc                                             20

<210> 30
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-116 gRNA targeting domain

<400> 30
acacggcagg gucaggguuc                                             20

<210> 31
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-16 gRNA targeting domain

<400> 31
gagaaucaaa aucggugaau                                             20

<210> 32
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-4 gRNA targeting domain

<400> 32
gcugguacac ggcaggguca                                             20

<210> 33
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-49 gRNA targeting domain

<400> 33
cucagcuggu acacggc                                                17

<210> 34
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-2 gRNA targeting domain

<400> 34
ugguacacgg caggguc                                                17

<210> 35
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-30 gRNA targeting domain

<400> 35
gcuagacaug aggucua                                                17

<210> 36
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-43 gRNA targeting domain

<400> 36
gucagauuug uugcucc                                                17

<210> 37
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-23 gRNA targeting domain

<400> 37
ucagcuggua cacggca                                                17

<210> 38
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-34 gRNA targeting domain

<400> 38
gcagacagac uugucac                                                17

<210> 39
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-25 gRNA targeting domain

<400> 39
gguacacggc aggguca                                                17

<210> 40
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-128 gRNA targeting domain

<400> 40
cuucaagagc aacagugcug                                             20

<210> 41
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-105 gRNA targeting domain

<400> 41
agagcaacag ugcuguggcc                                             20

<210> 42
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-106 gRNA targeting domain

<400> 42
aaagucagau uuguugcucc                                             20

<210> 43
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-123 gRNA targeting domain

<400> 43
acaaaacugu gcuagacaug                                             20

<210> 44
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-64 gRNA targeting domain

<400> 44
aaacugugcu agacaug                                                17

<210> 45
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-97 gRNA targeting domain

<400> 45
ugugcuagac augaggucua                                             20

<210> 46
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-148 gRNA targeting domain

<400> 46
ggcuggggaa gaaggugucu uc                                          22

<210> 47
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-147 gRNA targeting domain

<400> 47
gcuggggaag aaggugucuu c                                           21

<210> 48
<211> 18
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-234 gRNA targeting domain

<400> 48
ggggaagaag gugucuuc                                               18

<210> 49
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-167 gRNA targeting domain

<400> 49
guuuugucug ugauauacac au                                          22

<210> 50
<211> 24
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-177 gRNA targeting domain

<400> 50
ggcagacaga cuugucacug gauu                                        24

<210> 51
<211> 23
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-176 gRNA targeting domain

<400> 51
gcagacagac uugucacugg auu                                         23

<210> 52
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-257 gRNA targeting domain

<400> 52
gacagacuug ucacuggauu                                             20

<210> 53
<211> 24
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-233 gRNA targeting domain

<400> 53
gugaauaggc agacagacuu guca                                        24

<210> 54
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-231 gRNA targeting domain

<400> 54
gaauaggcag acagacuugu ca                                          22

<210> 55
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-163 gRNA targeting domain

<400> 55
gagucucuca gcugguacac gg                                          22

<210> 56
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-241 gRNA targeting domain

<400> 56
gucucucagc ugguacacgg                                             20

<210> 57
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-179 gRNA targeting domain

<400> 57
gguacacggc agggucaggg uu                                          22

<210> 58
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRAC-178 gRNA targeting domain

<400> 58
guacacggca gggucagggu u                                           21

<210> 59
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-40 gRNA targeting domain

<400> 59
cacccagauc gucagcgccg                                             20

<210> 60
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-52 gRNA targeting domain

<400> 60
caaacacagc gaccucgggu                                             20

<210> 61
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-25 gRNA targeting domain

<400> 61
ugacgagugg acccaggaua                                             20

<210> 62
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-35 gRNA targeting domain

<400> 62
ggcucucgga gaaugacgag                                             20

<210> 63
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-50 gRNA targeting domain

<400> 63
ggccucggcg cugacgaucu                                             20

<210> 64
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-39 gRNA targeting domain

<400> 64
gaaaaacgug uucccacccg                                             20

<210> 65
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-49 gRNA targeting domain

<400> 65
augacgagug gacccaggau                                             20

<210> 66
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-51 gRNA targeting domain

<400> 66
aguccaguuc uacgggcucu                                             20

<210> 67
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-26 gRNA targeting domain

<400> 67
cgcugucaag uccaguucua                                             20

<210> 68
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-47 gRNA targeting domain

<400> 68
aucgucagcg ccgaggccug                                             20

<210> 69
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-45 gRNA targeting domain

<400> 69
ucaaacacag cgaccucggg                                             20

<210> 70
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-34 gRNA targeting domain

<400> 70
cguagaacug gacuugacag                                             20

<210> 71
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-227 gRNA targeting domain

<400> 71
aggccucggc gcugacgauc                                             20

<210> 72
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-41 gRNA targeting domain

<400> 72
ugacagcgga agugguugcg                                             20

<210> 73
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-30 gRNA targeting domain

<400> 73
uugacagcgg aagugguugc                                             20

<210> 74
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-206 gRNA targeting domain

<400> 74
ucuccgagag cccguagaac                                             20

<210> 75
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-32 gRNA targeting domain

<400> 75
cgggugggaa cacguuuuuc                                             20

<210> 76
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-276 gRNA targeting domain

<400> 76
gacagguuug gcccuauccu                                             20

<210> 77
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-274 gRNA targeting domain

<400> 77
gaucgucagc gccgaggccu                                             20

<210> 78
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-230 gRNA targeting domain

<400> 78
ggcucaaaca cagcgaccuc                                             20

<210> 79
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-235 gRNA targeting domain

<400> 79
ugagggucuc ggccaccuuc                                             20

<210> 80
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-38 gRNA targeting domain

<400> 80
aggcuucuac cccgaccacg                                             20

<210> 81
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-223 gRNA targeting domain

<400> 81
ccgaccacgu ggagcugagc                                             20

<210> 82
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-221 gRNA targeting domain

<400> 82
ugacagguuu ggcccuaucc                                             20

<210> 83
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-48 gRNA targeting domain

<400> 83
cuugacagcg gaagugguug                                             20

<210> 84
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-216 gRNA targeting domain

<400> 84
agaucgucag cgccgaggcc                                             20

<210> 85
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-210 gRNA targeting domain

<400> 85
gcgcugacga ucugggugac                                             20

<210> 86
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-268 gRNA targeting domain

<400> 86
ugagggcggg cugcuccuug                                             20

<210> 87
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-193 gRNA targeting domain

<400> 87
guugcggggg uucugccaga                                             20

<210> 88
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-246 gRNA targeting domain

<400> 88
agcucagcuc cacguggucg                                             20

<210> 89
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-228 gRNA targeting domain

<400> 89
gcggcugcuc aggcaguauc                                             20

<210> 90
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-43 gRNA targeting domain

<400> 90
gcggggguuc ugccagaagg                                             20

<210> 91
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-272 gRNA targeting domain

<400> 91
uggcucaaac acagcgaccu                                             20

<210> 92
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-33 gRNA targeting domain

<400> 92
acuggacuug acagcggaag                                             20

<210> 93
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-44 gRNA targeting domain

<400> 93
gacagcggaa gugguugcgg                                             20

<210> 94
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-211 gRNA targeting domain

<400> 94
gcugucaagu ccaguucuac                                             20

<210> 95
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-253 gRNA targeting domain

<400> 95
guaucuggag ucauugaggg                                             20

<210> 96
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-18 gRNA targeting domain

<400> 96
cucggcgcug acgaucu                                                17

<210> 97
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-6 gRNA targeting domain

<400> 97
ccucggcgcu gacgauc                                                17

<210> 98
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-85 gRNA targeting domain

<400> 98
ccgagagccc guagaac                                                17

<210> 99
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-129 gRNA targeting domain

<400> 99
ccagaucguc agcgccg                                                17

<210> 100
<211> 17
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-93 gRNA targeting domain

<400> 100
gaaugacgag uggaccc                                                17

<210> 101
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-415 gRNA targeting domain

<400> 101
gggugacagg uuuggcccua uc                                          22

<210> 102
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-414 gRNA targeting domain

<400> 102
ggugacaggu uuggcccuau c                                           21

<210> 103
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-310 gRNA targeting domain

<400> 103
gugacagguu uggcccuauc                                             20

<210> 104
<211> 18
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-308 gRNA targeting domain

<400> 104
gacagguuug gcccuauc                                               18

<210> 105
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-401 gRNA targeting domain

<400> 105
gauacugccu gagcagccgc cu                                          22

<210> 106
<211> 24
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-468 gRNA targeting domain

<400> 106
gaccacgugg agcugagcug gugg                                        24

<210> 107
<211> 18
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-462 gRNA targeting domain

<400> 107
guggagcuga gcuggugg                                               18

<210> 108
<211> 24
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-424 gRNA targeting domain

<400> 108
gggcgggcug cuccuugagg ggcu                                        24

<210> 109
<211> 23
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-423 gRNA targeting domain

<400> 109
ggcgggcugc uccuugaggg gcu                                         23

<210> 110
<211> 22
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-422 gRNA targeting domain

<400> 110
gcgggcugcu ccuugagggg cu                                          22

<210> 111
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-420 gRNA targeting domain

<400> 111
gggcugcucc uugaggggcu                                             20

<210> 112
<211> 19
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-419 gRNA targeting domain

<400> 112
ggcugcuccu ugaggggcu                                              19

<210> 113
<211> 18
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-418 gRNA targeting domain

<400> 113
gcugcuccuu gaggggcu                                               18

<210> 114
<211> 24
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-445 gRNA targeting domain

<400> 114
ggugaauggg aaggaggugc acag                                        24

<210> 115
<211> 23
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-444 gRNA targeting domain

<400> 115
gugaauggga aggaggugca cag                                         23

<210> 116
<211> 21
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC-442 gRNA targeting domain

<400> 116
gaaugggaag gaggugcaca g                                           21

<210> 117
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence 1

<400> 117
attcaccgat tttgattctc                                             20

<210> 118
<211> 20
<212> RNA
<213> Artificial Sequence

<220> 
<223> TRBC target sequence 2

<400> 118
agatcgtcag cgccgaggcc                                             20

<210> 119
<211> 25
<212> DNA
<213> Artificial Sequence

<220> 
<223> HBB splice site acceptor

<400> 119
ctgacctctt ctcttcctcc cacag                                       25

<210> 120
<211> 13
<212> DNA
<213> Artificial Sequence

<220> 
<223> IgG splice site acceptor

<400> 120
tttctctcca cag                                                    13

<210> 121
<211> 138
<212> PRT
<213> mus musculus

<220> 
<223> Mouse TCR alpha constant

<300> 
<308> Uniprot P01849
<309> 1986-07-21  

<400> 121
Pro Tyr Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro
 1               5                  10                  15      
Arg Ser Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln
            20                  25                  30          
Ile Asn Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys
        35                  40                  45              
Thr Val Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile
    50                  55                  60                  
Ala Trp Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu
65                  70                  75                  80  
Thr Asn Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu
                85                  90                  95      
Thr Glu Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu
            100                 105                 110         
Ser Val Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn
        115                 120                 125             
Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135             


<210> 122
<211> 136
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified mouse TCR alpha constant

<400> 122
Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser
 1               5                  10                  15      
Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn
            20                  25                  30          
Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Cys Val
        35                  40                  45              
Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp
    50                  55                  60                  
Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn
65                  70                  75                  80  
Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu
                85                  90                  95      
Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Leu Val
            100                 105                 110         
Ile Val Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu
        115                 120                 125             
Met Thr Leu Arg Leu Trp Ser Ser
    130                 135     


<210> 123
<211> 173
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified mouse TCR beta constant

<400> 123
Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser Leu Phe Glu Pro
 1               5                  10                  15      
Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu Val Cys Leu
            20                  25                  30          
Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Ala Tyr Lys
    50                  55                  60                  
Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala
65                  70                  75                  80  
Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe
                85                  90                  95      
His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser Pro Lys Pro
            100                 105                 110         
Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly
        115                 120                 125             
Ile Thr Ser Ala Ser Tyr Gln Gln Gly Val Leu Ser Ala Thr Ile Leu
    130                 135                 140                 
Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val Ser
145                 150                 155                 160 
Thr Leu Val Val Met Ala Met Val Lys Arg Lys Asn Ser
                165                 170             


<210> 124
<211> 611
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 5' homology arm

<400> 124
ctctatcaat gagagagcaa tctcctggta atgtgataga tttcccaact taatgccaac 60
ataccataaa cctcccattc tgctaatgcc cagcctaagt tggggagacc actccagatt 120
ccaagatgta cagtttgctt tgctgggcct ttttcccatg cctgccttta ctctgccaga 180
gttatattgc tggggttttg aagaagatcc tattaaataa aagaataagc agtattatta 240
agtagccctg catttcaggt ttccttgagt ggcaggccag gcctggccgt gaacgttcac 300
tgaaatcatg gcctcttggc caagattgat agcttgtgcc tgtccctgag tcccagtcca 360
tcacgagcag ctggtttcta agatgctatt tcccgtataa agcatgagac cgtgacttgc 420
cagccccaca gagccccgcc cttgtccatc actggcatct ggactccagc ctgggttggg 480
gcaaagaggg aaatgagatc atgtcctaac cctgatcctc ttgtcccaca gatatccaga 540
accctgaccc tgccgtgtac cagctgagag actctaaatc cagtgacaag tctgtctgcc 600
tattcaccga t                                                      611

<210> 125
<211> 628
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 3' homology arm

<400> 125
tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa 60
actgtgctag acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac 120
aaatctgact ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc 180
ttccccagcc caggtaaggg cagctttggt gccttcgcag gctgtttcct tgcttcagga 240
atggccaggt tctgcccaga gctctggtca atgatgtcta aaactcctct gattggtggt 300
ctcggcctta tccattgcca ccaaaaccct ctttttacta agaaacagtg agccttgttc 360
tggcagtcca gagaatgaca cgggaaaaaa gcagatgaag agaaggtggc aggagagggc 420
acgtggccca gcctcagtct ctccaactga gttcctgcct gcctgccttt gctcagactg 480
tttgcccctt actgctcttc taggcctcat tctaagcccc ttctccaagt tgcctctcct 540
tatttctccc tgtctgccaa aaaatctttc ccagctcact aagtcagtct cacgcagtca 600
ctcattaacc caccaatcac tgattgtg                                    628

<210> 126
<211> 347
<212> DNA
<213> Artificial Sequence

<220> 
<223> MND promoter

<400> 126
gaacagagaa acaggagaat atgggccaaa caggatatct gtggtaagca gttcctgccc 60
cggctcaggg ccaagaacag ttggaacagc agaatatggg ccaaacagga tatctgtggt 120
aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc 180
tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc 240
ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc 300
tccccgagct ctatataagc agagctcgtt tagtgaaccg tcagatc               347

<210> 127
<211> 544
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ef1alpha promoter with HTLV1 enhancer

<400> 127
ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 60
agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa 120
actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 180
atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 240
agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc 300
gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg 360
cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc 420
cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac 480
tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc 540
ctac                                                              544

<210> 128
<211> 66
<212> DNA
<213> Artificial Sequence

<220> 
<223> P2A nucleotide sequence

<400> 128
ggatctggag cgacgaattt tagtctactg aaacaagcgg gagacgtgga ggaaaaccct 60
ggacct                                                            66

<210> 129
<211> 112
<212> PRT
<213> homo sapiens

<220> 
<223> CD3 zeta

<400> 129
Arg Val Lys Phe Ser Arg Ser Ala Glu Pro Pro Ala Tyr Gln Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         


<210> 130
<211> 112
<212> PRT
<213> homo sapiens

<220> 
<223> CD3 zeta

<400> 130
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         


<210> 131
<211> 12
<212> PRT
<213> homo sapiens

<220> 
<223> spacer (IgG4hinge)

<400> 131
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10          


<210> 132
<211> 36
<212> DNA
<213> homo sapiens

<220> 
<223> spacer (IgG4hinge)

<400> 132
gaatctaagt acggaccgcc ctgcccccct tgccct                           36

<210> 133
<211> 119
<212> PRT
<213> homo sapiens

<220> 
<223> Hinge-CH3 spacer

<400> 133
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg
 1               5                  10                  15      
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
            20                  25                  30          
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
        35                  40                  45              
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
    50                  55                  60                  
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
65                  70                  75                  80  
Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
                85                  90                  95      
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
            100                 105                 110         
Leu Ser Leu Ser Leu Gly Lys
        115                 


<210> 134
<211> 229
<212> PRT
<213> homo sapiens

<220> 
<223> Hinge-CH2-CH3 spacer

<400> 134
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
 1               5                  10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220                 
Leu Ser Leu Gly Lys
225                 


<210> 135
<211> 282
<212> PRT
<213> homo sapiens

<220> 
<223> IgD-hinge-Fc

<400> 135
Arg Trp Pro Glu Ser Pro Lys Ala Gln Ala Ser Ser Val Pro Thr Ala
 1               5                  10                  15      
Gln Pro Gln Ala Glu Gly Ser Leu Ala Lys Ala Thr Thr Ala Pro Ala
            20                  25                  30          
Thr Thr Arg Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys
        35                  40                  45              
Glu Lys Glu Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro Glu Cys Pro
    50                  55                  60                  
Ser His Thr Gln Pro Leu Gly Val Tyr Leu Leu Thr Pro Ala Val Gln
65                  70                  75                  80  
Asp Leu Trp Leu Arg Asp Lys Ala Thr Phe Thr Cys Phe Val Val Gly
                85                  90                  95      
Ser Asp Leu Lys Asp Ala His Leu Thr Trp Glu Val Ala Gly Lys Val
            100                 105                 110         
Pro Thr Gly Gly Val Glu Glu Gly Leu Leu Glu Arg His Ser Asn Gly
        115                 120                 125             
Ser Gln Ser Gln His Ser Arg Leu Thr Leu Pro Arg Ser Leu Trp Asn
    130                 135                 140                 
Ala Gly Thr Ser Val Thr Cys Thr Leu Asn His Pro Ser Leu Pro Pro
145                 150                 155                 160 
Gln Arg Leu Met Ala Leu Arg Glu Pro Ala Ala Gln Ala Pro Val Lys
                165                 170                 175     
Leu Ser Leu Asn Leu Leu Ala Ser Ser Asp Pro Pro Glu Ala Ala Ser
            180                 185                 190         
Trp Leu Leu Cys Glu Val Ser Gly Phe Ser Pro Pro Asn Ile Leu Leu
        195                 200                 205             
Met Trp Leu Glu Asp Gln Arg Glu Val Asn Thr Ser Gly Phe Ala Pro
    210                 215                 220                 
Ala Arg Pro Pro Pro Gln Pro Gly Ser Thr Thr Phe Trp Ala Trp Ser
225                 230                 235                 240 
Val Leu Arg Val Pro Ala Pro Pro Ser Pro Gln Pro Ala Thr Tyr Thr
                245                 250                 255     
Cys Val Val Ser His Glu Asp Ser Arg Thr Leu Leu Asn Ala Ser Arg
            260                 265                 270         
Ser Leu Glu Val Ser Tyr Val Thr Asp His
        275                 280         


<210> 136
<211> 27
<212> PRT
<213> homo sapiens

<220> 
<223> CD28

<300> 
<308> No. P10747
<309> 1989-07-01  

<400> 136
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
 1               5                  10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val
            20                  25          


<210> 137
<211> 66
<212> PRT
<213> homo sapiens

<220> 
<223> CD28

<300> 
<308> No. P10747
<309> 1989-07-01  

<400> 137
Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn
 1               5                  10                  15      
Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu
            20                  25                  30          
Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly
        35                  40                  45              
Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe
    50                  55                  60                  
Trp Val
65      


<210> 138
<211> 41
<212> PRT
<213> homo sapiens

<220> 
<223> CD28

<300> 
<308> P10747
<309> 1989-07-01  

<400> 138
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr
 1               5                  10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser
        35                  40      


<210> 139
<211> 41
<212> PRT
<213> homo sapiens

<220> 
<223> CD28 (LL to GG)

<400> 139
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr
 1               5                  10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser
        35                  40      


<210> 140
<211> 42
<212> PRT
<213> homo sapiens

<220> 
<223> 4-1BB

<300> 
<308> Q07011.1
<309> 1995-02-01  

<400> 140
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met
 1               5                  10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
        35                  40          


<210> 141
<211> 112
<212> PRT
<213> homo sapiens

<220> 
<223> CD3 zeta

<400> 141
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly
 1               5                  10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg
            100                 105                 110         


<210> 142
<211> 96
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary gRNA complementary domain

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223>  n is a, c, g, or u

<400> 142
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugc                           96

<210> 143
<211> 104
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary gRNA complementary domain

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223> n is a, c, g, or u

<400> 143
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcugaaa agcauagcaa guuaaaauaa 60
ggcuaguccg uuaucaacuu gaaaaagugg caccgagucg gugc                  104

<210> 144
<211> 106
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary gRNA complementary domain

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223> n is a, c, g, or u

<400> 144
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuggaa acagcauagc aaguuaaaau 60
aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc                106

<210> 145
<211> 116
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary gRNA complementary domain

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223> n is a, c, g, or u

<400> 145
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuguuu uggaaacaaa acagcauagc 60
aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc     116

<210> 146
<211> 96
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary gRNA

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223> n is a, c, g, or u

<400> 146
nnnnnnnnnn nnnnnnnnnn guauuagagc uagaaauagc aaguuaauau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugc                           96

<210> 147
<211> 96
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary gRNA

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223> n is a, c, g, or u

<400> 147
nnnnnnnnnn nnnnnnnnnn guuuaagagc uagaaauagc aaguuuaaau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugc                           96

<210> 148
<211> 116
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary gRNA

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223> n is a, c, g, or u

<400> 148
nnnnnnnnnn nnnnnnnnnn guauuagagc uaugcuguau uggaaacaau acagcauagc 60
aaguuaauau aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc     116

<210> 149
<211> 47
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary proximal and tail domain

<400> 149
aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcu               47

<210> 150
<211> 49
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary proximal and tail domain

<400> 150
aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cgguggugc             49

<210> 151
<211> 51
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary proximal and tail domain

<400> 151
aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcggau c          51

<210> 152
<211> 31
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary proximal and tail domain

<400> 152
aaggcuaguc cguuaucaac uugaaaaagu g                                31

<210> 153
<211> 18
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary proximal and tail domain

<400> 153
aaggcuaguc cguuauca                                               18

<210> 154
<211> 12
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary proximal and tail domain

<400> 154
aaggcuaguc cg                                                     12

<210> 155
<211> 102
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary chimeric gRNA

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223> n is a, c, g, or u

<400> 155
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc 60
cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu                    102

<210> 156
<211> 102
<212> RNA
<213> Artificial Sequence

<220> 
<223> exemplary chimeric gRNA

<220> 
<221> modified_base  
<222> (1)...(20)
<223> a, c, u, g, unknown or other

<220> 
<221> misc_feature   
<222> (1)...(20)
<223> n is a, c, g, or u

<400> 156
nnnnnnnnnn nnnnnnnnnn guuuuaguac ucuggaaaca gaaucuacua aaacaaggca 60
aaaugccgug uuuaucucgu caacuuguug gcgagauuuu uu                    102

<210> 157
<211> 1344
<212> PRT
<213> Artificial Sequence

<220> 
<223> Streptococcus mutans Cas9

<400> 157
Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly
 1               5                  10                  15      
Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met Lys
            20                  25                  30          
Val Leu Gly Asn Thr Asp Lys Ser His Ile Glu Lys Asn Leu Leu Gly
        35                  40                  45              
Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu Lys
    50                  55                  60                  
Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu Tyr
65                  70                  75                  80  
Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser Phe
                85                  90                  95      
Phe His Arg Leu Glu Asp Ser Phe Leu Val Thr Glu Asp Lys Arg Gly
            100                 105                 110         
Glu Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Lys Tyr His
        115                 120                 125             
Glu Asn Phe Pro Thr Ile Tyr His Leu Arg Gln Tyr Leu Ala Asp Asn
    130                 135                 140                 
Pro Glu Lys Val Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His Ile
145                 150                 155                 160 
Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Lys Phe Asp Thr Arg
                165                 170                 175     
Asn Asn Asp Val Gln Arg Leu Phe Gln Glu Phe Leu Ala Val Tyr Asp
            180                 185                 190         
Asn Thr Phe Glu Asn Ser Ser Leu Gln Glu Gln Asn Val Gln Val Glu
        195                 200                 205             
Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg Val
    210                 215                 220                 
Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Arg Phe Ala Glu Phe
225                 230                 235                 240 
Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe Glu
                245                 250                 255     
Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Glu Glu
            260                 265                 270         
Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu Leu
        275                 280                 285             
Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly Ile
    290                 295                 300                 
Leu Thr Val Thr Asp Val Gly Thr Lys Ala Pro Leu Ser Ala Ser Met
305                 310                 315                 320 
Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys Gln
                325                 330                 335     
Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser Asp
            340                 345                 350         
Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn Gln
        355                 360                 365             
Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu Gly
    370                 375                 380                 
Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg Lys
385                 390                 395                 400 
Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gln
                405                 410                 415     
Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe Leu
            420                 425                 430         
Ala Asp Asn Gln Asp Arg Ile Glu Lys Leu Leu Thr Phe Arg Ile Pro
        435                 440                 445             
Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp Leu
    450                 455                 460                 
Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu Ile
465                 470                 475                 480 
Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr Asn
                485                 490                 495     
Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser Leu
            500                 505                 510         
Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr
        515                 520                 525             
Lys Thr Glu Gln Gly Lys Thr Ala Phe Phe Asp Ala Asn Met Lys Gln
    530                 535                 540                 
Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr Lys Asp
545                 550                 555                 560 
Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile Val
                565                 570                 575     
Asp Leu Thr Gly Leu Asp Lys Glu Asn Lys Val Phe Asn Ala Ser Tyr
            580                 585                 590         
Gly Thr Tyr His Asp Leu Cys Lys Ile Leu Asp Lys Asp Phe Leu Asp
        595                 600                 605             
Asn Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu Thr
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr Ser
625                 630                 635                 640 
Asp Leu Leu Thr Lys Glu Gln Val Lys Lys Leu Glu Arg Arg His Tyr
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg Asn
            660                 665                 670         
Lys Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Asn
        675                 680                 685             
Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser Phe
    690                 695                 700                 
Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn Leu
705                 710                 715                 720 
Asn Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys Gly
                725                 730                 735     
Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met Gly
            740                 745                 750         
His Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln Phe
        755                 760                 765             
Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu Thr
    770                 775                 780                 
Asp Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro Val
785                 790                 795                 800 
Glu Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu Gln
                805                 810                 815     
Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr Leu
            820                 825                 830         
Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp
        835                 840                 845             
Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg Gly
    850                 855                 860                 
Lys Ser Asp Asp Val Pro Ser Lys Asp Val Val Arg Lys Met Lys Ser
865                 870                 875                 880 
Tyr Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys Phe
                885                 890                 895     
Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp Lys
            900                 905                 910         
Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys
        915                 920                 925             
His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Thr Asp Glu
    930                 935                 940                 
Asn Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser Asn
945                 950                 955                 960 
Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg Glu
                965                 970                 975     
Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Ile
            980                 985                 990         
Gly Lys Ala Leu Leu Gly Val Tyr Pro Gln Leu Glu Pro Glu Phe Val
        995                 1000                1005            
Tyr Gly Asp Tyr Pro His Phe His Gly His Lys Glu Asn Lys Ala Thr
    1010                1015                1020                
Ala Lys Lys Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Lys Asp
1025                1030                1035               1040 
Asp Val Arg Thr Asp Lys Asn Gly Glu Ile Ile Trp Lys Lys Asp Glu
                1045                1050                1055    
His Ile Ser Asn Ile Lys Lys Val Leu Ser Tyr Pro Gln Val Asn Ile
            1060                1065                1070        
Val Lys Lys Val Glu Glu Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile
        1075                1080                1085            
Leu Pro Lys Gly Asn Ser Asp Lys Leu Ile Pro Arg Lys Thr Lys Lys
    1090                1095                1100                
Phe Tyr Trp Asp Thr Lys Lys Tyr Gly Gly Phe Asp Ser Pro Ile Val
1105                1110                1115               1120 
Ala Tyr Ser Ile Leu Val Ile Ala Asp Ile Glu Lys Gly Lys Ser Lys
                1125                1130                1135    
Lys Leu Lys Thr Val Lys Ala Leu Val Gly Val Thr Ile Met Glu Lys
            1140                1145                1150        
Met Thr Phe Glu Arg Asp Pro Val Ala Phe Leu Glu Arg Lys Gly Tyr
        1155                1160                1165            
Arg Asn Val Gln Glu Glu Asn Ile Ile Lys Leu Pro Lys Tyr Ser Leu
    1170                1175                1180                
Phe Lys Leu Glu Asn Gly Arg Lys Arg Leu Leu Ala Ser Ala Arg Glu
1185                1190                1195               1200 
Leu Gln Lys Gly Asn Glu Ile Val Leu Pro Asn His Leu Gly Thr Leu
                1205                1210                1215    
Leu Tyr His Ala Lys Asn Ile His Lys Val Asp Glu Pro Lys His Leu
            1220                1225                1230        
Asp Tyr Val Asp Lys His Lys Asp Glu Phe Lys Glu Leu Leu Asp Val
        1235                1240                1245            
Val Ser Asn Phe Ser Lys Lys Tyr Thr Leu Ala Glu Gly Asn Leu Glu
    1250                1255                1260                
Lys Ile Lys Glu Leu Tyr Ala Gln Asn Asn Gly Glu Asp Leu Lys Glu
1265                1270                1275               1280 
Leu Ala Ser Ser Phe Ile Asn Leu Leu Thr Phe Thr Ala Ile Gly Ala
                1285                1290                1295    
Pro Ala Thr Phe Lys Phe Phe Asp Lys Asn Ile Asp Arg Lys Arg Tyr
            1300                1305                1310        
Thr Ser Thr Thr Glu Ile Leu Asn Ala Thr Leu Ile His Gln Ser Ile
        1315                1320                1325            
Thr Gly Leu Tyr Glu Thr Arg Ile Asp Leu Asn Lys Leu Gly Gly Asp
    1330                1335                1340                


<210> 158
<211> 1367
<212> PRT
<213> Artificial Sequence

<220> 
<223> Streptococcus pyogenes Cas9

<400> 158
Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly
 1               5                  10                  15      
Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys
            20                  25                  30          
Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly
        35                  40                  45              
Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys
    50                  55                  60                  
Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr
65                  70                  75                  80  
Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe
                85                  90                  95      
Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His
            100                 105                 110         
Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His
        115                 120                 125             
Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser
    130                 135                 140                 
Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met
145                 150                 155                 160 
Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp
                165                 170                 175     
Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn
            180                 185                 190         
Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys
        195                 200                 205             
Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu
    210                 215                 220                 
Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu
225                 230                 235                 240 
Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp
                245                 250                 255     
Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp
            260                 265                 270         
Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu
        275                 280                 285             
Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile
    290                 295                 300                 
Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met
305                 310                 315                 320 
Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala
                325                 330                 335     
Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp
            340                 345                 350         
Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln
        355                 360                 365             
Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly
    370                 375                 380                 
Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys
385                 390                 395                 400 
Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly
                405                 410                 415     
Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu
            420                 425                 430         
Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro
        435                 440                 445             
Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met
    450                 455                 460                 
Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val
465                 470                 475                 480 
Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn
                485                 490                 495     
Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu
            500                 505                 510         
Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr
        515                 520                 525             
Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys
    530                 535                 540                 
Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val
545                 550                 555                 560 
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser
                565                 570                 575     
Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr
            580                 585                 590         
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn
        595                 600                 605             
Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu
    610                 615                 620                 
Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His
625                 630                 635                 640 
Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr
                645                 650                 655     
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys
            660                 665                 670         
Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala
        675                 680                 685             
Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys
    690                 695                 700                 
Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His
705                 710                 715                 720 
Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile
                725                 730                 735     
Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg
            740                 745                 750         
His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr
        755                 760                 765             
Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu
    770                 775                 780                 
Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val
785                 790                 795                 800 
Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln
                805                 810                 815     
Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu
            820                 825                 830         
Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp
        835                 840                 845             
Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly
    850                 855                 860                 
Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn
865                 870                 875                 880 
Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe
                885                 890                 895     
Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys
            900                 905                 910         
Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys
        915                 920                 925             
His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu
    930                 935                 940                 
Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys
945                 950                 955                 960 
Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu
                965                 970                 975     
Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val
            980                 985                 990         
Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val
        995                 1000                1005            
Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys Ser
    1010                1015                1020                
Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn
1025                1030                1035               1040 
Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu Ile
                1045                1050                1055    
Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile Val
            1060                1065                1070        
Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser Met
        1075                1080                1085            
Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly Phe
    1090                1095                1100                
Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala
1105                1110                1115               1120 
Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro
                1125                1130                1135    
Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly Lys
            1140                1145                1150        
Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile Met
        1155                1160                1165            
Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys
    1170                1175                1180                
Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr
1185                1190                1195               1200 
Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala
                1205                1210                1215    
Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val
            1220                1225                1230        
Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro
        1235                1240                1245            
Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His Tyr
    1250                1255                1260                
Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile
1265                1270                1275               1280 
Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys His
                1285                1290                1295    
Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu Phe
            1300                1305                1310        
Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp Thr
        1315                1320                1325            
Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp Ala
    1330                1335                1340                
Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile Asp
1345                1350                1355               1360 
Leu Ser Gln Leu Gly Gly Asp
                1365        


<210> 159
<211> 1387
<212> PRT
<213> Artificial Sequence

<220> 
<223> Streptococcus thermophilus Cas9

<400> 159
Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly
 1               5                  10                  15      
Trp Ala Val Thr Thr Asp Asn Tyr Lys Val Pro Ser Lys Lys Met Lys
            20                  25                  30          
Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu Gly
        35                  40                  45              
Val Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu Lys
    50                  55                  60                  
Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu Tyr
65                  70                  75                  80  
Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala Phe
                85                  90                  95      
Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg Asp
            100                 105                 110         
Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr His
        115                 120                 125             
Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp Ser
    130                 135                 140                 
Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His Met
145                 150                 155                 160 
Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser Lys
                165                 170                 175     
Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr Asn
            180                 185                 190         
Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu Glu
        195                 200                 205             
Glu Ile Val Lys Asp Lys Ile Ser Lys Leu Glu Lys Lys Asp Arg Ile
    210                 215                 220                 
Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu Phe
225                 230                 235                 240 
Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Arg Lys Cys Phe Asn
                245                 250                 255     
Leu Asp Glu Lys Ala Ser Leu His Phe Ser Lys Glu Ser Tyr Asp Glu
            260                 265                 270         
Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp Val
        275                 280                 285             
Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly Phe
    290                 295                 300                 
Leu Thr Val Thr Asp Asn Glu Thr Glu Ala Pro Leu Ser Ser Ala Met
305                 310                 315                 320 
Ile Lys Arg Tyr Asn Glu His Lys Glu Asp Leu Ala Leu Leu Lys Glu
                325                 330                 335     
Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr Asn Glu Val Phe Lys Asp
            340                 345                 350         
Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn Gln
        355                 360                 365             
Glu Asp Phe Tyr Val Tyr Leu Lys Lys Leu Leu Ala Glu Phe Glu Gly
    370                 375                 380                 
Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg Lys
385                 390                 395                 400 
Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu Gln
                405                 410                 415     
Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe Leu
            420                 425                 430         
Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro
        435                 440                 445             
Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp Ser
    450                 455                 460                 
Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp Val
465                 470                 475                 480 
Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr Ser
                485                 490                 495     
Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser Leu
            500                 505                 510         
Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg Phe
        515                 520                 525             
Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln Lys
    530                 535                 540                 
Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr Asp
545                 550                 555                 560 
Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Tyr Gly Tyr Asp Gly Ile
                565                 570                 575     
Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr Tyr
            580                 585                 590         
His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp Ser
        595                 600                 605             
Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile Phe
    610                 615                 620                 
Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn Ile
625                 630                 635                 640 
Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr Gly
                645                 650                 655     
Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu Lys
            660                 665                 670         
Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser Asn
        675                 680                 685             
Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys Lys
    690                 695                 700                 
Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Glu Asp Lys Gly Asn Ile
705                 710                 715                 720 
Lys Glu Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys Gly
                725                 730                 735     
Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met Gly
            740                 745                 750         
Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn Gln
        755                 760                 765             
Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg Leu
    770                 775                 780                 
Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn Ile
785                 790                 795                 800 
Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp Arg
                805                 810                 815     
Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Asp
            820                 825                 830         
Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile Ile
        835                 840                 845             
Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu Val
    850                 855                 860                 
Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu Glu
865                 870                 875                 880 
Val Val Lys Lys Arg Lys Thr Phe Trp Tyr Gln Leu Leu Lys Ser Lys
                885                 890                 895     
Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly
            900                 905                 910         
Gly Leu Ser Pro Glu Asp Lys Ala Gly Phe Ile Gln Arg Gln Leu Val
        915                 920                 925             
Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu Lys
    930                 935                 940                 
Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg Ala Val Arg Thr Val Lys
945                 950                 955                 960 
Ile Ile Thr Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Asp Phe
                965                 970                 975     
Glu Leu Tyr Lys Val Arg Glu Ile Asn Asp Phe His His Ala His Asp
            980                 985                 990         
Ala Tyr Leu Asn Ala Val Val Ala Ser Ala Leu Leu Lys Lys Tyr Pro
        995                 1000                1005            
Lys Leu Glu Pro Glu Phe Val Tyr Gly Asp Tyr Pro Lys Tyr Asn Ser
    1010                1015                1020                
Phe Arg Glu Arg Lys Ser Ala Thr Glu Lys Val Tyr Phe Tyr Ser Asn
1025                1030                1035               1040 
Ile Met Asn Ile Phe Lys Lys Ser Ile Ser Leu Ala Asp Gly Arg Val
                1045                1050                1055    
Ile Glu Arg Pro Leu Ile Glu Val Asn Glu Glu Thr Gly Glu Ser Val
            1060                1065                1070        
Trp Asn Lys Glu Ser Asp Leu Ala Thr Val Arg Arg Val Leu Ser Tyr
        1075                1080                1085            
Pro Gln Val Asn Val Val Lys Lys Val Glu Glu Gln Asn His Gly Leu
    1090                1095                1100                
Asp Arg Gly Lys Pro Lys Gly Leu Phe Asn Ala Asn Leu Ser Ser Lys
1105                1110                1115               1120 
Pro Lys Pro Asn Ser Asn Glu Asn Leu Val Gly Ala Lys Glu Tyr Leu
                1125                1130                1135    
Asp Pro Lys Lys Tyr Gly Gly Tyr Ala Gly Ile Ser Asn Ser Phe Thr
            1140                1145                1150        
Val Leu Val Lys Gly Thr Ile Glu Lys Gly Ala Lys Lys Lys Ile Thr
        1155                1160                1165            
Asn Val Leu Glu Phe Gln Gly Ile Ser Ile Leu Asp Arg Ile Asn Tyr
    1170                1175                1180                
Arg Lys Asp Lys Leu Asn Phe Leu Leu Glu Lys Gly Tyr Lys Asp Ile
1185                1190                1195               1200 
Glu Leu Ile Ile Glu Leu Pro Lys Tyr Ser Leu Phe Glu Leu Ser Asp
                1205                1210                1215    
Gly Ser Arg Arg Met Leu Ala Ser Ile Leu Ser Thr Asn Asn Lys Arg
            1220                1225                1230        
Gly Glu Ile His Lys Gly Asn Gln Ile Phe Leu Ser Gln Lys Phe Val
        1235                1240                1245            
Lys Leu Leu Tyr His Ala Lys Arg Ile Ser Asn Thr Ile Asn Glu Asn
    1250                1255                1260                
His Arg Lys Tyr Val Glu Asn His Lys Lys Glu Phe Glu Glu Leu Phe
1265                1270                1275               1280 
Tyr Tyr Ile Leu Glu Phe Asn Glu Asn Tyr Val Gly Ala Lys Lys Asn
                1285                1290                1295    
Gly Lys Leu Leu Asn Ser Ala Phe Gln Ser Trp Gln Asn His Ser Ile
            1300                1305                1310        
Asp Glu Leu Cys Ser Ser Phe Ile Gly Pro Thr Gly Ser Glu Arg Lys
        1315                1320                1325            
Gly Leu Phe Glu Leu Thr Ser Arg Gly Ser Ala Ala Asp Phe Glu Phe
    1330                1335                1340                
Leu Gly Val Lys Ile Pro Arg Tyr Arg Asp Tyr Thr Pro Ser Ser Leu
1345                1350                1355               1360 
Leu Lys Asp Ala Thr Leu Ile His Gln Ser Val Thr Gly Leu Tyr Glu
                1365                1370                1375    
Thr Arg Ile Asp Leu Ala Lys Leu Gly Glu Gly
            1380                1385        


<210> 160
<211> 1333
<212> PRT
<213> Artificial Sequence

<220> 
<223> Listeria innocua Cas9

<400> 160
Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly
 1               5                  10                  15      
Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met Lys
            20                  25                  30          
Ile Ala Gly Asp Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp Gly
        35                  40                  45              
Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Ala Asp Arg Arg Met Ala
    50                  55                  60                  
Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser Tyr
65                  70                  75                  80  
Leu Gln Gly Ile Phe Ala Glu Glu Met Ser Lys Thr Asp Ala Asn Phe
                85                  90                  95      
Phe Cys Arg Leu Ser Asp Ser Phe Tyr Val Asp Asn Glu Lys Arg Asn
            100                 105                 110         
Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Glu Tyr His
        115                 120                 125             
Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn Ser
    130                 135                 140                 
Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His Ile
145                 150                 155                 160 
Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr Gln
                165                 170                 175     
Asn Thr Ser Val Asp Gly Ile Tyr Lys Gln Phe Ile Gln Thr Tyr Asn
            180                 185                 190         
Gln Val Phe Ala Ser Gly Ile Glu Asp Gly Ser Leu Lys Lys Leu Glu
        195                 200                 205             
Asp Asn Lys Asp Val Ala Lys Ile Leu Val Glu Lys Val Thr Arg Lys
    210                 215                 220                 
Glu Lys Leu Glu Arg Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser Ala
225                 230                 235                 240 
Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly Asn
                245                 250                 255     
Phe Gln Lys Pro Phe Asp Leu Ile Glu Lys Ser Asp Ile Glu Cys Ala
            260                 265                 270         
Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ser Leu Leu Ala Leu Ile Gly
        275                 280                 285             
Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Ala Tyr Ser Ala
    290                 295                 300                 
Val Val Leu Ser Ser Ile Ile Thr Val Ala Glu Thr Glu Thr Asn Ala
305                 310                 315                 320 
Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Thr His Glu Glu Asp
                325                 330                 335     
Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys His Tyr
            340                 345                 350         
Glu Glu Ile Phe Ser Asn Thr Glu Lys His Gly Tyr Ala Gly Tyr Ile
        355                 360                 365             
Asp Gly Lys Thr Lys Gln Ala Asp Phe Tyr Lys Tyr Met Lys Met Thr
    370                 375                 380                 
Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu Lys
385                 390                 395                 400 
Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile Pro
                405                 410                 415     
His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln Ala
            420                 425                 430         
Lys Tyr Tyr Pro Phe Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser Leu
        435                 440                 445             
Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly Gln
    450                 455                 460                 
Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg Pro
465                 470                 475                 480 
Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp Phe
                485                 490                 495     
Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn Val
            500                 505                 510         
Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Leu Val Tyr Asn Glu
        515                 520                 525             
Leu Thr Lys Val Arg Tyr Ile Asn Asp Gln Gly Lys Thr Ser Tyr Phe
    530                 535                 540                 
Ser Gly Gln Glu Lys Glu Gln Ile Phe Asn Asp Leu Phe Lys Gln Lys
545                 550                 555                 560 
Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Met Ser
                565                 570                 575     
His Val Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn Ser
            580                 585                 590         
Ser Tyr Ser Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln Glu
        595                 600                 605             
Ile Leu Asp Asn Pro Val Asn Thr Glu Met Leu Glu Asn Ile Val Lys
    610                 615                 620                 
Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu Gln
625                 630                 635                 640 
Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys Lys Leu Glu Arg
                645                 650                 655     
Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Met Gly
            660                 665                 670         
Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met Asn
        675                 680                 685             
Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser Asn
    690                 695                 700                 
Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Thr Thr Ala Asp
705                 710                 715                 720 
Lys Asp Ile Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala Ile
                725                 730                 735     
Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Ser
            740                 745                 750         
Val Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg Glu
        755                 760                 765             
Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr Lys
    770                 775                 780                 
Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu
785                 790                 795                 800 
His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu Tyr
                805                 810                 815     
Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp Ile
            820                 825                 830         
His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser Phe
        835                 840                 845             
Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala Gly
    850                 855                 860                 
Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg Lys
865                 870                 875                 880 
Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser Lys
                885                 890                 895     
Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Glu
            900                 905                 910         
Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg Gln
        915                 920                 925             
Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr Glu
    930                 935                 940                 
Lys Asp Asp His Gly Asn Thr Met Lys Gln Val Arg Ile Val Thr Leu
945                 950                 955                 960 
Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr Lys
                965                 970                 975     
Val Arg Asp Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn
            980                 985                 990         
Gly Val Val Ala Asn Thr Leu Leu Lys Val Tyr Pro Gln Leu Glu Pro
        995                 1000                1005            
Glu Phe Val Tyr Gly Asp Tyr His Gln Phe Asp Trp Phe Lys Ala Asn
    1010                1015                1020                
Lys Ala Thr Ala Lys Lys Gln Phe Tyr Thr Asn Ile Met Leu Phe Phe
1025                1030                1035               1040 
Ala Gln Lys Asp Arg Ile Ile Asp Glu Asn Gly Glu Ile Leu Trp Asp
                1045                1050                1055    
Lys Lys Tyr Leu Asp Thr Val Lys Lys Val Met Ser Tyr Arg Gln Met
            1060                1065                1070        
Asn Ile Val Lys Lys Thr Glu Ile Gln Lys Gly Glu Phe Ser Lys Ala
        1075                1080                1085            
Thr Ile Lys Pro Lys Gly Asn Ser Ser Lys Leu Ile Pro Arg Lys Thr
    1090                1095                1100                
Asn Trp Asp Pro Met Lys Tyr Gly Gly Leu Asp Ser Pro Asn Met Ala
1105                1110                1115               1120 
Tyr Ala Val Val Ile Glu Tyr Ala Lys Gly Lys Asn Lys Leu Val Phe
                1125                1130                1135    
Glu Lys Lys Ile Ile Arg Val Thr Ile Met Glu Arg Lys Ala Phe Glu
            1140                1145                1150        
Lys Asp Glu Lys Ala Phe Leu Glu Glu Gln Gly Tyr Arg Gln Pro Lys
        1155                1160                1165            
Val Leu Ala Lys Leu Pro Lys Tyr Thr Leu Tyr Glu Cys Glu Glu Gly
    1170                1175                1180                
Arg Arg Arg Met Leu Ala Ser Ala Asn Glu Ala Gln Lys Gly Asn Gln
1185                1190                1195               1200 
Gln Val Leu Pro Asn His Leu Val Thr Leu Leu His His Ala Ala Asn
                1205                1210                1215    
Cys Glu Val Ser Asp Gly Lys Ser Leu Asp Tyr Ile Glu Ser Asn Arg
            1220                1225                1230        
Glu Met Phe Ala Glu Leu Leu Ala His Val Ser Glu Phe Ala Lys Arg
        1235                1240                1245            
Tyr Thr Leu Ala Glu Ala Asn Leu Asn Lys Ile Asn Gln Leu Phe Glu
    1250                1255                1260                
Gln Asn Lys Glu Gly Asp Ile Lys Ala Ile Ala Gln Ser Phe Val Asp
1265                1270                1275               1280 
Leu Met Ala Phe Asn Ala Met Gly Ala Pro Ala Ser Phe Lys Phe Phe
                1285                1290                1295    
Glu Thr Thr Ile Glu Arg Lys Arg Tyr Asn Asn Leu Lys Glu Leu Leu
            1300                1305                1310        
Asn Ser Thr Ile Ile Tyr Gln Ser Ile Thr Gly Leu Tyr Glu Ser Arg
        1315                1320                1325            
Lys Arg Leu Asp Asp
    1330            


<210> 161
<211> 1082
<212> PRT
<213> Artificial Sequence

<220> 
<223> Neisseria meningitidis Cas9

<400> 161
Met Ala Ala Phe Lys Pro Asn Ser Ile Asn Tyr Ile Leu Gly Leu Asp
 1               5                  10                  15      
Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Glu
            20                  25                  30          
Glu Asn Pro Ile Arg Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg
        35                  40                  45              
Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu
    50                  55                  60                  
Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu
65                  70                  75                  80  
Arg Thr Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asn
                85                  90                  95      
Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln
            100                 105                 110         
Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser
        115                 120                 125             
Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg
    130                 135                 140                 
Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys
145                 150                 155                 160 
Gly Val Ala Gly Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr
                165                 170                 175     
Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile
            180                 185                 190         
Arg Asn Gln Arg Ser Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu
        195                 200                 205             
Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn
    210                 215                 220                 
Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met
225                 230                 235                 240 
Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly
                245                 250                 255     
His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr
            260                 265                 270         
Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile
        275                 280                 285             
Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr
    290                 295                 300                 
Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala
305                 310                 315                 320 
Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg
                325                 330                 335     
Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala
            340                 345                 350         
Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys
        355                 360                 365             
Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr
    370                 375                 380                 
Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys
385                 390                 395                 400 
Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser
                405                 410                 415     
Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val
            420                 425                 430         
Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile
        435                 440                 445             
Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu
    450                 455                 460                 
Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala
465                 470                 475                 480 
Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly
                485                 490                 495     
Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser
            500                 505                 510         
Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys
        515                 520                 525             
Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe
    530                 535                 540                 
Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu
545                 550                 555                 560 
Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly
                565                 570                 575     
Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe
            580                 585                 590         
Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly
        595                 600                 605             
Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn
    610                 615                 620                 
Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu
625                 630                 635                 640 
Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys
                645                 650                 655     
Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr
            660                 665                 670         
Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr
        675                 680                 685             
Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn
    690                 695                 700                 
Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp
705                 710                 715                 720 
Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala
                725                 730                 735     
Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala
            740                 745                 750         
Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln
        755                 760                 765             
Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met
    770                 775                 780                 
Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala
785                 790                 795                 800 
Asp Thr Leu Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser
                805                 810                 815     
Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg
            820                 825                 830         
Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys
        835                 840                 845             
Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu
    850                 855                 860                 
Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg
865                 870                 875                 880 
Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys
                885                 890                 895     
Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys
            900                 905                 910         
Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val
        915                 920                 925             
Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn
    930                 935                 940                 
Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr
945                 950                 955                 960 
Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp
                965                 970                 975     
Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp
            980                 985                 990         
Asp Ser Phe Asn Phe Lys Phe Ser Leu His Pro Asn Asp Leu Val Glu
        995                 1000                1005            
Val Ile Thr Lys Lys Ala Arg Met Phe Gly Tyr Phe Ala Ser Cys His
    1010                1015                1020                
Arg Gly Thr Gly Asn Ile Asn Ile Arg Ile His Asp Leu Asp His Lys
1025                1030                1035               1040 
Ile Gly Lys Asn Gly Ile Leu Glu Gly Ile Gly Val Lys Thr Ala Leu
                1045                1050                1055    
Ser Phe Gln Lys Tyr Gln Ile Asp Glu Leu Gly Lys Glu Ile Arg Pro
            1060                1065                1070        
Cys Arg Leu Lys Lys Arg Pro Pro Val Arg
        1075                1080        


<210> 162
<211> 1368
<212> PRT
<213> Artificial Sequence

<220> 
<223> Streptococcus pyogenes Cas9

<400> 162
Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val
 1               5                  10                  15      
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe
            20                  25                  30          
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile
        35                  40                  45              
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu
    50                  55                  60                  
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys
65                  70                  75                  80  
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser
                85                  90                  95      
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys
            100                 105                 110         
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr
        115                 120                 125             
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp
    130                 135                 140                 
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His
145                 150                 155                 160 
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro
                165                 170                 175     
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr
            180                 185                 190         
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala
        195                 200                 205             
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn
    210                 215                 220                 
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn
225                 230                 235                 240 
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe
                245                 250                 255     
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp
            260                 265                 270         
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp
        275                 280                 285             
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp
    290                 295                 300                 
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser
305                 310                 315                 320 
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys
                325                 330                 335     
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe
            340                 345                 350         
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser
        355                 360                 365             
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp
    370                 375                 380                 
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg
385                 390                 395                 400 
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu
                405                 410                 415     
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe
            420                 425                 430         
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile
        435                 440                 445             
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp
    450                 455                 460                 
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu
465                 470                 475                 480 
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr
                485                 490                 495     
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser
            500                 505                 510         
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys
        515                 520                 525             
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln
    530                 535                 540                 
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr
545                 550                 555                 560 
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp
                565                 570                 575     
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly
            580                 585                 590         
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp
        595                 600                 605             
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr
    610                 615                 620                 
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala
625                 630                 635                 640 
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr
                645                 650                 655     
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp
            660                 665                 670         
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe
        675                 680                 685             
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe
    690                 695                 700                 
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu
705                 710                 715                 720 
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly
                725                 730                 735     
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly
            740                 745                 750         
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln
        755                 760                 765             
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile
    770                 775                 780                 
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro
785                 790                 795                 800 
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu
                805                 810                 815     
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg
            820                 825                 830         
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys
        835                 840                 845             
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg
    850                 855                 860                 
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys
865                 870                 875                 880 
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys
                885                 890                 895     
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp
            900                 905                 910         
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr
        915                 920                 925             
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp
    930                 935                 940                 
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser
945                 950                 955                 960 
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg
                965                 970                 975     
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val
            980                 985                 990         
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe
        995                 1000                1005            
Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys
    1010                1015                1020                
Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser
1025                1030                1035               1040 
Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu
                1045                1050                1055    
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile
            1060                1065                1070        
Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser
        1075                1080                1085            
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly
    1090                1095                1100                
Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys Leu Ile
1105                1110                1115               1120 
Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Asp Ser
                1125                1130                1135    
Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly
            1140                1145                1150        
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile
        1155                1160                1165            
Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala
    1170                1175                1180                
Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys
1185                1190                1195               1200 
Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser
                1205                1210                1215    
Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr
            1220                1225                1230        
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser
        1235                1240                1245            
Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His
    1250                1255                1260                
Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val
1265                1270                1275               1280 
Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys
                1285                1290                1295    
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu
            1300                1305                1310        
Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp
        1315                1320                1325            
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu Asp
    1330                1335                1340                
Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile
1345                1350                1355               1360 
Asp Leu Ser Gln Leu Gly Gly Asp
                1365            


<210> 163
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence 2

<400> 163
gagaatcaaa atcggtgaat                                             20

<210> 164
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRBC target sequence 2

<400> 164
ggcctcggcg ctgacgatct                                             20

<210> 165
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 3

<400> 165
gagaatcaaa atcggtgaat agg                                         23

<210> 166
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 4

<400> 166
ttcaaaacct gtcagtgatt ggg                                         23

<210> 167
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 5

<400> 167
tgtgctagac atgaggtcta tgg                                         23

<210> 168
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 6

<400> 168
cgtcatgagc agattaaacc cgg                                         23

<210> 169
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 7

<400> 169
tcagggttct ggatatctgt ggg                                         23

<210> 170
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 8

<400> 170
gtcagggttc tggatatctg tgg                                         23

<210> 171
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 9

<400> 171
ttcggaaccc aatcactgac agg                                         23

<210> 172
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 10

<400> 172
taaacccggc cactttcagg agg                                         23

<210> 173
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 11

<400> 173
aaagtcagat ttgttgctcc agg                                         23

<210> 174
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 12

<400> 174
aacaaatgtg tcacaaagta agg                                         23

<210> 175
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 13

<400> 175
tggatttaga gtctctcagc tgg                                         23

<210> 176
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 14

<400> 176
taggcagaca gacttgtcac tgg                                         23

<210> 177
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 15

<400> 177
agctggtaca cggcagggtc agg                                         23

<210> 178
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 16

<400> 178
gctggtacac ggcagggtca ggg                                         23

<210> 179
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 17

<400> 179
tctctcagct ggtacacggc agg                                         23

<210> 180
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 18

<400> 180
tttcaaaacc tgtcagtgat tgg                                         23

<210> 181
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 19

<400> 181
gattaaaccc ggccactttc agg                                         23

<210> 182
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 20

<400> 182
ctcgaccagc ttgacatcac agg                                         23

<210> 183
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 21

<400> 183
agagtctctc agctggtaca cgg                                         23

<210> 184
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 22

<400> 184
ctctcagctg gtacacggca ggg                                         23

<210> 185
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 23

<400> 185
aagttcctgt gatgtcaagc tgg                                         23

<210> 186
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 24

<400> 186
atcctcctcc tgaaagtggc cgg                                         23

<210> 187
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 25

<400> 187
tgctcatgac gctgcggctg tgg                                         23

<210> 188
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 26

<400> 188
acaaaactgt gctagacatg agg                                         23

<210> 189
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 27

<400> 189
atttgtttga gaatcaaaat cgg                                         23

<210> 190
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 28

<400> 190
catcacagga actttctaaa agg                                         23

<210> 191
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 29

<400> 191
gtcgagaaaa gctttgaaac agg                                         23

<210> 192
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 30

<400> 192
ccactttcag gaggaggatt cgg                                         23

<210> 193
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 31

<400> 193
ctgacaggtt ttgaaagttt agg                                         23

<210> 194
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 32

<400> 194
agctttgaaa caggtaagac agg                                         23

<210> 195
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 33

<400> 195
tggaataatg ctgttgttga agg                                         23

<210> 196
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 34

<400> 196
agagcaacag tgctgtggcc tgg                                         23

<210> 197
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 35

<400> 197
ctgtggtcca gctgaggtga ggg                                         23

<210> 198
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 36

<400> 198
ctgcggctgt ggtccagctg agg                                         23

<210> 199
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 37

<400> 199
tgtggtccag ctgaggtgag ggg                                         23

<210> 200
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 38

<400> 200
cttcttcccc agcccaggta agg                                         23

<210> 201
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 39

<400> 201
acacggcagg gtcagggttc tgg                                         23

<210> 202
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 40

<400> 202
cttcaagagc aacagtgctg tgg                                         23

<210> 203
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 41

<400> 203
ctggggaaga aggtgtcttc tgg                                         23

<210> 204
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 42

<400> 204
tcctcctcct gaaagtggcc ggg                                         23

<210> 205
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 43

<400> 205
ttaatctgct catgacgctg cgg                                         23

<210> 206
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 44

<400> 206
acccggccac tttcaggagg agg                                         23

<210> 207
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 45

<400> 207
ttcttcccca gcccaggtaa ggg                                         23

<210> 208
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 46

<400> 208
cttacctggg ctggggaaga agg                                         23

<210> 209
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 47

<400> 209
gacaccttct tccccagccc agg                                         23

<210> 210
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 48

<400> 210
gctgtggtcc agctgaggtg agg                                         23

<210> 211
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC target sequence with PAM 49

<400> 211
ccgaatcctc ctcctgaaag tgg                                         23

<210> 212
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRBC target sequence with PAM 3

<400> 212
gctgtcaagt ccagttctac ggg                                         23

<210> 213
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 1

<400> 213
ctatggactt caagagcaac agtgctgt                                    28

<210> 214
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 2

<400> 214
ctcatgtcta gcacagtttt gtctgtga                                    28

<210> 215
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 3

<400> 215
gtgctgtggc ctggagcaac aaatctga                                    28

<210> 216
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 4

<400> 216
ttgctcttga agtccataga cctcatgt                                    28

<210> 217
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 5

<400> 217
ctgtggcctg gagcaacaaa tctgact                                     27

<210> 218
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 6

<400> 218
ctgttgctct tgaagtccat agacctca                                    28

<210> 219
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 7

<400> 219
ctgtggcctg gagcaacaaa tctgactt                                    28

<210> 220
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 8

<400> 220
ctgactttgc atgtgcaaac gccttcaa                                    28

<210> 221
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 9

<400> 221
ttgttgctcc aggccacagc actgttgc                                    28

<210> 222
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 10

<400> 222
tgaaagtggc cgggtttaat ctgctcat                                    28

<210> 223
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 11

<400> 223
aggaggattc ggaacccaat cactgaca                                    28

<210> 224
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC ZFN target sequence 12

<400> 224
gaggaggatt cggaacccaa tcactgac                                    28

<210> 225
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRBC ZFN target sequence 1

<400> 225
ccgtagaact ggacttgaca gcggaagt                                    28

<210> 226
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRBC ZFN target sequence 2

<400> 226
tctcggagaa tgacgagtgg acccagga                                    28

<210> 227
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 50 bp 5' Homology Arm

<400> 227
agctgagaga ctctaaatcc agtgacaagt ctgtctgcct attcaccgat            50

<210> 228
<211> 100
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 100 bp 5' Homology Arm

<400> 228
ctgatcctct tgtcccacag atatccagaa ccctgaccct gccgtgtacc agctgagaga 60
ctctaaatcc agtgacaagt ctgtctgcct attcaccgat                       100

<210> 229
<211> 200
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 200 bp 5' Homology Arm

<400> 229
gtgacttgcc agccccacag agccccgccc ttgtccatca ctggcatctg gactccagcc 60
tgggttgggg caaagaggga aatgagatca tgtcctaacc ctgatcctct tgtcccacag 120
atatccagaa ccctgaccct gccgtgtacc agctgagaga ctctaaatcc agtgacaagt 180
ctgtctgcct attcaccgat                                             200

<210> 230
<211> 300
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 300 bp 5' Homology Arm

<400> 230
cctcttggcc aagattgata gcttgtgcct gtccctgagt cccagtccat cacgagcagc 60
tggtttctaa gatgctattt cccgtataaa gcatgagacc gtgacttgcc agccccacag 120
agccccgccc ttgtccatca ctggcatctg gactccagcc tgggttgggg caaagaggga 180
aatgagatca tgtcctaacc ctgatcctct tgtcccacag atatccagaa ccctgaccct 240
gccgtgtacc agctgagaga ctctaaatcc agtgacaagt ctgtctgcct attcaccgat 300


<210> 231
<211> 400
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 400 bp 5' Homology Arm

<400> 231
attaaataaa agaataagca gtattattaa gtagccctgc atttcaggtt tccttgagtg 60
gcaggccagg cctggccgtg aacgttcact gaaatcatgg cctcttggcc aagattgata 120
gcttgtgcct gtccctgagt cccagtccat cacgagcagc tggtttctaa gatgctattt 180
cccgtataaa gcatgagacc gtgacttgcc agccccacag agccccgccc ttgtccatca 240
ctggcatctg gactccagcc tgggttgggg caaagaggga aatgagatca tgtcctaacc 300
ctgatcctct tgtcccacag atatccagaa ccctgaccct gccgtgtacc agctgagaga 360
ctctaaatcc agtgacaagt ctgtctgcct attcaccgat                       400

<210> 232
<211> 500
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 500 bp 5' Homology Arm

<400> 232
ctccagattc caagatgtac agtttgcttt gctgggcctt tttcccatgc ctgcctttac 60
tctgccagag ttatattgct ggggttttga agaagatcct attaaataaa agaataagca 120
gtattattaa gtagccctgc atttcaggtt tccttgagtg gcaggccagg cctggccgtg 180
aacgttcact gaaatcatgg cctcttggcc aagattgata gcttgtgcct gtccctgagt 240
cccagtccat cacgagcagc tggtttctaa gatgctattt cccgtataaa gcatgagacc 300
gtgacttgcc agccccacag agccccgccc ttgtccatca ctggcatctg gactccagcc 360
tgggttgggg caaagaggga aatgagatca tgtcctaacc ctgatcctct tgtcccacag 420
atatccagaa ccctgaccct gccgtgtacc agctgagaga ctctaaatcc agtgacaagt 480
ctgtctgcct attcaccgat                                             500

<210> 233
<211> 600
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 600 bp 5' Homology Arm

<400> 233
agagagcaat ctcctggtaa tgtgatagat ttcccaactt aatgccaaca taccataaac 60
ctcccattct gctaatgccc agcctaagtt ggggagacca ctccagattc caagatgtac 120
agtttgcttt gctgggcctt tttcccatgc ctgcctttac tctgccagag ttatattgct 180
ggggttttga agaagatcct attaaataaa agaataagca gtattattaa gtagccctgc 240
atttcaggtt tccttgagtg gcaggccagg cctggccgtg aacgttcact gaaatcatgg 300
cctcttggcc aagattgata gcttgtgcct gtccctgagt cccagtccat cacgagcagc 360
tggtttctaa gatgctattt cccgtataaa gcatgagacc gtgacttgcc agccccacag 420
agccccgccc ttgtccatca ctggcatctg gactccagcc tgggttgggg caaagaggga 480
aatgagatca tgtcctaacc ctgatcctct tgtcccacag atatccagaa ccctgaccct 540
gccgtgtacc agctgagaga ctctaaatcc agtgacaagt ctgtctgcct attcaccgat 600


<210> 234
<211> 50
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 50 bp 3' Homology Arm

<400> 234
tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat            50

<210> 235
<211> 100
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 100 bp 3' Homology Arm

<400> 235
tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa 60
actgtgctag acatgaggtc tatggacttc aagagcaaca                       100

<210> 236
<211> 200
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 200 bp 3' Homology Arm

<400> 236
tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa 60
actgtgctag acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac 120
aaatctgact ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc 180
ttccccagcc caggtaaggg                                             200

<210> 237
<211> 300
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 300 bp 3' Homology Arm

<400> 237
tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa 60
actgtgctag acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac 120
aaatctgact ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc 180
ttccccagcc caggtaaggg cagctttggt gccttcgcag gctgtttcct tgcttcagga 240
atggccaggt tctgcccaga gctctggtca atgatgtcta aaactcctct gattggtggt 300


<210> 238
<211> 400
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 400 bp 3' Homology Arm

<400> 238
tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa 60
actgtgctag acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac 120
aaatctgact ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc 180
ttccccagcc caggtaaggg cagctttggt gccttcgcag gctgtttcct tgcttcagga 240
atggccaggt tctgcccaga gctctggtca atgatgtcta aaactcctct gattggtggt 300
ctcggcctta tccattgcca ccaaaaccct ctttttacta agaaacagtg agccttgttc 360
tggcagtcca gagaatgaca cgggaaaaaa gcagatgaag                       400

<210> 239
<211> 500
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 500 bp 3' Homology Arm

<400> 239
tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa 60
actgtgctag acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac 120
aaatctgact ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc 180
ttccccagcc caggtaaggg cagctttggt gccttcgcag gctgtttcct tgcttcagga 240
atggccaggt tctgcccaga gctctggtca atgatgtcta aaactcctct gattggtggt 300
ctcggcctta tccattgcca ccaaaaccct ctttttacta agaaacagtg agccttgttc 360
tggcagtcca gagaatgaca cgggaaaaaa gcagatgaag agaaggtggc aggagagggc 420
acgtggccca gcctcagtct ctccaactga gttcctgcct gcctgccttt gctcagactg 480
tttgcccctt actgctcttc                                             500

<210> 240
<211> 600
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRAC 600 bp 3' Homology Arm

<400> 240
tttgattctc aaacaaatgt gtcacaaagt aaggattctg atgtgtatat cacagacaaa 60
actgtgctag acatgaggtc tatggacttc aagagcaaca gtgctgtggc ctggagcaac 120
aaatctgact ttgcatgtgc aaacgccttc aacaacagca ttattccaga agacaccttc 180
ttccccagcc caggtaaggg cagctttggt gccttcgcag gctgtttcct tgcttcagga 240
atggccaggt tctgcccaga gctctggtca atgatgtcta aaactcctct gattggtggt 300
ctcggcctta tccattgcca ccaaaaccct ctttttacta agaaacagtg agccttgttc 360
tggcagtcca gagaatgaca cgggaaaaaa gcagatgaag agaaggtggc aggagagggc 420
acgtggccca gcctcagtct ctccaactga gttcctgcct gcctgccttt gctcagactg 480
tttgcccctt actgctcttc taggcctcat tctaagcccc ttctccaagt tgcctctcct 540
tatttctccc tgtctgccaa aaaatctttc ccagctcact aagtcagtct cacgcagtca 600


<210> 241
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 241
Asn Ile Gln Lys Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Ala Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 242
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 242
His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 243
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 243
Tyr Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 244
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 244
Asp Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 245
<211> 142
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 245
Pro Asn Ile Gln Lys Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser
 1               5                  10                  15      
Lys Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln
            20                  25                  30          
Thr Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys
        35                  40                  45              
Thr Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val
    50                  55                  60                  
Ala Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn
65                  70                  75                  80  
Ser Ile Ile Pro Ala Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys
                85                  90                  95      
Asp Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn
            100                 105                 110         
Phe Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val
        115                 120                 125             
Ala Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140         


<210> 246
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 246
Asn Ile Gln Lys Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Ala Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 247
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 247
His Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 248
<211> 140
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 248
Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser
 1               5                  10                  15      
Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn
            20                  25                  30          
Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys Val
        35                  40                  45              
Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp
    50                  55                  60                  
Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile
65                  70                  75                  80  
Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp Val
                85                  90                  95      
Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln
            100                 105                 110         
Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly
        115                 120                 125             
Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140 


<210> 249
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 249
Asn Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 250
<211> 141
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR alpha constant

<400> 250
Asp Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys
 1               5                  10                  15      
Ser Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr
            20                  25                  30          
Asn Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Cys
        35                  40                  45              
Val Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala
    50                  55                  60                  
Trp Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser
65                  70                  75                  80  
Ile Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp
                85                  90                  95      
Val Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe
            100                 105                 110         
Gln Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala
        115                 120                 125             
Gly Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser
    130                 135                 140     


<210> 251
<211> 177
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR beta constant

<400> 251
Glu Asp Leu Asn Lys Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro
 1               5                  10                  15      
Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu
            20                  25                  30          
Ala Thr Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu Lys
    50                  55                  60                  
Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu
65                  70                  75                  80  
Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys
                85                  90                  95      
Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp
            100                 105                 110         
Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg
        115                 120                 125             
Ala Asp Cys Gly Phe Thr Ser Val Ser Tyr Gln Gln Gly Val Leu Ser
    130                 135                 140                 
Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala
145                 150                 155                 160 
Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp
                165                 170                 175     
Phe
    


<210> 252
<211> 180
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR beta constant

<400> 252
Thr Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu
 1               5                  10                  15      
Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys
            20                  25                  30          
Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val
        35                  40                  45              
Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu
    50                  55                  60                  
Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg
65                  70                  75                  80  
Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg
                85                  90                  95      
Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln
            100                 105                 110         
Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly
        115                 120                 125             
Arg Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu
    130                 135                 140                 
Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr
145                 150                 155                 160 
Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys
                165                 170                 175     
Asp Ser Arg Gly
            180 


<210> 253
<211> 180
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR beta constant

<400> 253
Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu
 1               5                  10                  15      
Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys
            20                  25                  30          
Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val
        35                  40                  45              
Asn Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu
    50                  55                  60                  
Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg
65                  70                  75                  80  
Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg
                85                  90                  95      
Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln
            100                 105                 110         
Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly
        115                 120                 125             
Arg Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu
    130                 135                 140                 
Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr
145                 150                 155                 160 
Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys
                165                 170                 175     
Asp Ser Arg Gly
            180 


<210> 254
<211> 179
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR beta constant

<400> 254
Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu Pro
 1               5                  10                  15      
Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu
            20                  25                  30          
Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu Lys
    50                  55                  60                  
Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu
65                  70                  75                  80  
Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys
                85                  90                  95      
Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp
            100                 105                 110         
Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg
        115                 120                 125             
Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser
    130                 135                 140                 
Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala
145                 150                 155                 160 
Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp
                165                 170                 175     
Ser Arg Gly
            


<210> 255
<211> 180
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR beta constant

<400> 255
Thr Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu
 1               5                  10                  15      
Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys
            20                  25                  30          
Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val
        35                  40                  45              
Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu
    50                  55                  60                  
Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg
65                  70                  75                  80  
Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg
                85                  90                  95      
Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln
            100                 105                 110         
Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly
        115                 120                 125             
Arg Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu
    130                 135                 140                 
Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr
145                 150                 155                 160 
Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys
                165                 170                 175     
Asp Ser Arg Gly
            180 


<210> 256
<211> 180
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human TCR beta constant

<400> 256
Leu Glu Asp Leu Lys Asn Val Phe Pro Pro Glu Val Ala Val Phe Glu
 1               5                  10                  15      
Pro Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys
            20                  25                  30          
Leu Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val
        35                  40                  45              
Asn Gly Lys Glu Val His Ser Gly Val Cys Thr Asp Pro Gln Pro Leu
    50                  55                  60                  
Lys Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg
65                  70                  75                  80  
Leu Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg
                85                  90                  95      
Cys Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln
            100                 105                 110         
Asp Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly
        115                 120                 125             
Arg Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu
    130                 135                 140                 
Ser Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr
145                 150                 155                 160 
Ala Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys
                165                 170                 175     
Asp Ser Arg Gly
            180 

