                         SEQUENCE LISTING

<110>  GENETHON
       INSERM
       Universit d'Evry-val-d'Essone
       SORBONNE UNIVERSITE
       ASSOCIATION INSTITUT DE MYOLOGIE
 
<120>  HYBRID RECOMBINANT ADENO-ASSOCIATED VIRUS SEROTYPE BETWEEN AAV9 
       AND AAVrh74 WITH REDUCED LIVER TROPISM

<130>  BCT190089 QT

<150>  EP 18305399.0
<151>  2018-04-05

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  736
<212>  PRT
<213>  adeno-associated virus 9

<400>  1

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe Ser 
    450                 455                 460                 


Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile Pro 
465                 470                 475                 480 


Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln Asn 
                485                 490                 495     


Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu Asn 
            500                 505                 510         


Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His Lys 
        515                 520                 525             


Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe Gly 
    530                 535                 540                 


Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met Ile 
545                 550                 555                 560 


Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu Ser 
                565                 570                 575     


Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala Gln 
            580                 585                 590         


Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp Gln 
        595                 600                 605             


Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His 
    610                 615                 620                 


Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Met 
625                 630                 635                 640 


Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala 
                645                 650                 655     


Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr 
            660                 665                 670         


Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln 
        675                 680                 685             


Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn 
    690                 695                 700                 


Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly Val 
705                 710                 715                 720 


Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735     


<210>  2
<211>  738
<212>  PRT
<213>  adeno-associated virus rh74

<400>  2

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln Lys Gln Asp Asn Gly Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Ser Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile 
145                 150                 155                 160 


Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln 
                165                 170                 175     


Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro 
            180                 185                 190         


Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly 
        195                 200                 205             


Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser 
    210                 215                 220                 


Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val 
225                 230                 235                 240 


Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His 
                245                 250                 255     


Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp 
            260                 265                 270         


Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn 
        275                 280                 285             


Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn 
    290                 295                 300                 


Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn 
305                 310                 315                 320 


Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala 
                325                 330                 335     


Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln 
            340                 345                 350         


Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe 
        355                 360                 365             


Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn 
    370                 375                 380                 


Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr 
385                 390                 395                 400 


Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr 
                405                 410                 415     


Asn Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser 
            420                 425                 430         


Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu 
        435                 440                 445             


Ser Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu 
    450                 455                 460                 


Phe Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp 
465                 470                 475                 480 


Leu Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser 
                485                 490                 495     


Gln Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His 
            500                 505                 510         


Leu Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr 
        515                 520                 525             


His Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met 
    530                 535                 540                 


Phe Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val 
545                 550                 555                 560 


Met Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr 
                565                 570                 575     


Glu Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Ala Ala 
            580                 585                 590         


Pro Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val 
        595                 600                 605             


Trp Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile 
    610                 615                 620                 


Pro His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe 
625                 630                 635                 640 


Gly Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val 
                645                 650                 655     


Pro Ala Asp Pro Pro Thr Thr Phe Asn Gln Ala Lys Leu Ala Ser Phe 
            660                 665                 670         


Ile Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu 
        675                 680                 685             


Leu Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr 
    690                 695                 700                 


Ser Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu 
705                 710                 715                 720 


Gly Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg 
                725                 730                 735     


Asn Leu 
        


<210>  3
<211>  737
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide  AAV9-rh74 hybrid capsid

<400>  3

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe 
    450                 455                 460                 


Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu 
465                 470                 475                 480 


Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln 
                485                 490                 495     


Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His Leu 
            500                 505                 510         


Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr His 
        515                 520                 525             


Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe 
    530                 535                 540                 


Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met 
545                 550                 555                 560 


Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu 
                565                 570                 575     


Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Ala Ala Pro 
            580                 585                 590         


Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp 
        595                 600                 605             


Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro 
    610                 615                 620                 


His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly 
625                 630                 635                 640 


Met Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro 
                645                 650                 655     


Ala Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile 
            660                 665                 670         


Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu 
        675                 680                 685             


Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser 
    690                 695                 700                 


Asn Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly 
705                 710                 715                 720 


Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn 
                725                 730                 735     


Leu 
    


<210>  4
<211>  737
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide AAVrh74-9 hybrid capsid

<400>  4

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln Lys Gln Asp Asn Gly Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Ser Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile 
145                 150                 155                 160 


Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln 
                165                 170                 175     


Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro 
            180                 185                 190         


Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly 
        195                 200                 205             


Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser 
    210                 215                 220                 


Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val 
225                 230                 235                 240 


Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His 
                245                 250                 255     


Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp 
            260                 265                 270         


Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn 
        275                 280                 285             


Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn 
    290                 295                 300                 


Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn 
305                 310                 315                 320 


Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala 
                325                 330                 335     


Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln 
            340                 345                 350         


Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe 
        355                 360                 365             


Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn 
    370                 375                 380                 


Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr 
385                 390                 395                 400 


Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr 
                405                 410                 415     


Asn Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser 
            420                 425                 430         


Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu 
        435                 440                 445             


Ser Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe 
    450                 455                 460                 


Ser Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile 
465                 470                 475                 480 


Pro Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln 
                485                 490                 495     


Asn Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu 
            500                 505                 510         


Asn Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His 
        515                 520                 525             


Lys Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe 
    530                 535                 540                 


Gly Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met 
545                 550                 555                 560 


Ile Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu 
                565                 570                 575     


Ser Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala 
            580                 585                 590         


Gln Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp 
        595                 600                 605             


Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro 
    610                 615                 620                 


His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly 
625                 630                 635                 640 


Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro 
                645                 650                 655     


Ala Asp Pro Pro Thr Thr Phe Asn Gln Ala Lys Leu Ala Ser Phe Ile 
            660                 665                 670         


Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu 
        675                 680                 685             


Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser 
    690                 695                 700                 


Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly 
705                 710                 715                 720 


Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn 
                725                 730                 735     


Leu 
    


<210>  5
<211>  2214
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic polynucleotide encoding AAV9-rh74 hybrid capsid


<220>
<221>  CDS
<222>  (1)..(2211)

<400>  5
atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctt agt         48
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser           
1               5                   10                  15                

gaa gga att cgc gag tgg tgg gct ttg aaa cct gga gcc cct caa ccc         96
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro           
            20                  25                  30                    

aag gca aat caa caa cat caa gac aac gct cga ggt ctt gtg ctt ccg        144
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro           
        35                  40                  45                        

ggt tac aaa tac ctt gga ccc ggc aac gga ctc gac aag ggg gag ccg        192
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro           
    50                  55                  60                            

gtc aac gca gca gac gcg gcg gcc ctc gag cac gac aag gcc tac gac        240
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp           
65                  70                  75                  80            

cag cag ctc aag gcc gga gac aac ccg tac ctc aag tac aac cac gcc        288
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala           
                85                  90                  95                

gac gcc gag ttc cag gag cgg ctc aaa gaa gat acg tct ttt ggg ggc        336
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly           
            100                 105                 110                   

aac ctc ggg cga gca gtc ttc cag gcc aaa aag agg ctt ctt gaa cct        384
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro           
        115                 120                 125                       

ctt ggt ctg gtt gag gaa gcg gct aag acg gct cct gga aag aag agg        432
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg           
    130                 135                 140                           

cct gta gag cag tct cct cag gaa ccg gac tcc tcc gcg ggt att ggc        480
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly           
145                 150                 155                 160           

aaa tcg ggt gca cag ccc gct aaa aag aga ctc aat ttc ggt cag act        528
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr           
                165                 170                 175               

ggc gac aca gag tca gtc cca gac cct caa cca atc gga gaa cct ccc        576
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro           
            180                 185                 190                   

gca gcc ccc tca ggt gtg gga tct ctt aca atg gct tca ggt ggt ggc        624
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly           
        195                 200                 205                       

gca cca gtg gca gac aat aac gaa ggt gcc gat gga gtg ggt agt tcc        672
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser           
    210                 215                 220                           

tcg gga aat tgg cat tgc gat tcc caa tgg ctg ggg gac aga gtc atc        720
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile           
225                 230                 235                 240           

acc acc agc acc cga acc tgg gcc ctg ccc acc tac aac aat cac ctc        768
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu           
                245                 250                 255               

tac aag caa atc tcc aac agc aca tct gga gga tct tca aat gac aac        816
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn           
            260                 265                 270                   

gcc tac ttc ggc tac agc acc ccc tgg ggg tat ttt gac ttc aac aga        864
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg           
        275                 280                 285                       

ttc cac tgc cac ttc tca cca cgt gac tgg cag cga ctc atc aac aac        912
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn           
    290                 295                 300                           

aac tgg gga ttc cgg cct aag cga ctc aac ttc aag ctc ttc aac att        960
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile           
305                 310                 315                 320           

cag gtc aaa gag gtt acg gac aac aat gga gtc aag acc atc gcc aat       1008
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn           
                325                 330                 335               

aac ctt acc agc acg gtc cag gtc ttc acg gac tca gac tat cag ctc       1056
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu           
            340                 345                 350                   

ccg tac gtg ctc ggg tcg gct cac gag ggc tgc ctc ccg ccg ttc cca       1104
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro           
        355                 360                 365                       

gcg gac gtt ttc atg att cct cag tac ggg tat ctg acg ctt aat gat       1152
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp           
    370                 375                 380                           

gga agc cag gcc gtg ggt cgt tcg tcc ttt tac tgc ctg gaa tat ttc       1200
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe           
385                 390                 395                 400           

ccg tcg caa atg cta aga acg ggt aac aac ttc cag ttc agc tac gag       1248
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu           
                405                 410                 415               

ttt gag aac gta cct ttc cat agc agc tac gct cac agc caa agc ctg       1296
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu           
            420                 425                 430                   

gac cga cta atg aat cca ctc atc gac caa tac ttg tac tat ctc tca       1344
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser           
        435                 440                 445                       

cgg act caa agc acg ggc ggt act gca gga act cag cag ttg cta ttt       1392
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe           
    450                 455                 460                           

tct cag gcc ggg cct aac aac atg tcg gct cag gcc aag aac tgg cta       1440
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu           
465                 470                 475                 480           

ccc ggt ccc tgc tac cgg cag caa cgc gtc tcc acg aca ctg tcg cag       1488
Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln           
                485                 490                 495               

aac aac aac agc aac ttt gcc tgg acg ggt gcc acc aag tat cat ctg       1536
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His Leu           
            500                 505                 510                   

aat ggc aga gac tct ctg gtg aat cct ggc gtt gcc atg gct acc cac       1584
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr His           
        515                 520                 525                       

aag gac gac gaa gag cga ttt ttt cca tcc agc gga gtc tta atg ttt       1632
Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe           
    530                 535                 540                           

ggg aaa cag gga gct gga aaa gac aac gtg gac tat agc agc gtg atg       1680
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met           
545                 550                 555                 560           

cta acc agc gag gaa gaa ata aag acc acc aac cca gtg gcc aca gaa       1728
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu           
                565                 570                 575               

cag tac ggc gtg gtg gcc gat aac ctg caa cag caa aac gcc gct cct       1776
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Ala Ala Pro           
            580                 585                 590                   

att gta ggg gcc gtc aat agt caa gga gcc tta cct ggc atg gtg tgg       1824
Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp           
        595                 600                 605                       

cag aac aga gat gtg tac ctg caa gga ccc att tgg gcc aaa att cct       1872
Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro           
    610                 615                 620                           

cac acg gac ggc aac ttt cac cct tct ccg ctg atg gga ggg ttt gga       1920
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly           
625                 630                 635                 640           

atg aag cac ccg cct cct cag atc ctc atc aaa aac aca cct gta cct       1968
Met Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro           
                645                 650                 655               

gcg gat cct cca acg gcc ttc aac aag gac aag ctg aac tct ttc atc       2016
Ala Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile           
            660                 665                 670                   

acc cag tat tct act ggc caa gtc agc gtg gag atc gag tgg gag ctg       2064
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu           
        675                 680                 685                       

cag aag gaa aac agc aag cgc tgg aac ccg gag atc cag tac act tcc       2112
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser           
    690                 695                 700                           

aac tat tac aag tct aat aat gtt gaa ttt gct gtt aat act gaa ggt       2160
Asn Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly           
705                 710                 715                 720           

gta tat agt gaa ccc cgc ccc att ggc acc aga tac ctg act cgt aat       2208
Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn           
                725                 730                 735               

ctg taa                                                               2214
Leu                                                                       
                                                                          


<210>  6
<211>  737
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe 
    450                 455                 460                 


Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu 
465                 470                 475                 480 


Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln 
                485                 490                 495     


Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His Leu 
            500                 505                 510         


Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr His 
        515                 520                 525             


Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe 
    530                 535                 540                 


Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met 
545                 550                 555                 560 


Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu 
                565                 570                 575     


Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gln Gln Asn Ala Ala Pro 
            580                 585                 590         


Ile Val Gly Ala Val Asn Ser Gln Gly Ala Leu Pro Gly Met Val Trp 
        595                 600                 605             


Gln Asn Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro 
    610                 615                 620                 


His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly 
625                 630                 635                 640 


Met Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro 
                645                 650                 655     


Ala Asp Pro Pro Thr Ala Phe Asn Lys Asp Lys Leu Asn Ser Phe Ile 
            660                 665                 670         


Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu 
        675                 680                 685             


Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser 
    690                 695                 700                 


Asn Tyr Tyr Lys Ser Asn Asn Val Glu Phe Ala Val Asn Thr Glu Gly 
705                 710                 715                 720 


Val Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn 
                725                 730                 735     


Leu 
    


<210>  7
<211>  2214
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic polynucleotide encoding AAVrh74-9 hybrid capsid


<220>
<221>  CDS
<222>  (1)..(2211)

<400>  7
atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctc tct         48
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser           
1               5                   10                  15                

gag ggc att cgc gag tgg tgg gac ctg aaa cct gga gcc ccg aaa ccc         96
Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro           
            20                  25                  30                    

aaa gcc aac cag caa aag cag gac aac ggc cgg ggt ctg gtg ctt cct        144
Lys Ala Asn Gln Gln Lys Gln Asp Asn Gly Arg Gly Leu Val Leu Pro           
        35                  40                  45                        

ggc tac aag tac ctc gga ccc ttc aac gga ctc gac aag ggg gag ccc        192
Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro           
    50                  55                  60                            

gtc aac gcg gcg gac gca gcg gcc ctc gag cac gac aag gcc tac gac        240
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp           
65                  70                  75                  80            

cag cag ctc caa gcg ggt gac aat ccg tac ctg cgg tat aat cac gcc        288
Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala           
                85                  90                  95                

gac gcc gag ttt cag gag cgt ctg caa gaa gat acg tct ttt ggg ggc        336
Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly           
            100                 105                 110                   

aac ctc ggg cgc gca gtc ttc cag gcc aaa aag cgg gtt ctc gaa cct        384
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro           
        115                 120                 125                       

ctg ggc ctg gtt gaa tcg ccg gtt aag acg gct cct gga aag aag aga        432
Leu Gly Leu Val Glu Ser Pro Val Lys Thr Ala Pro Gly Lys Lys Arg           
    130                 135                 140                           

ccg gta gag cca tca ccc cag cgc tct cca gac tcc tct acg ggc atc        480
Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile           
145                 150                 155                 160           

ggc aag aaa ggc cag cag ccc gca aaa aag aga ctc aat ttt ggg cag        528
Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln           
                165                 170                 175               

act ggc gac tca gag tca gtc ccc gac cct caa cca atc gga gaa cca        576
Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro           
            180                 185                 190                   

cca gca ggc ccc tct ggt ctg gga tct ggt aca atg gct gca ggc ggt        624
Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly           
        195                 200                 205                       

ggc gct cca atg gca gac aat aac gaa ggc gcc gac gga gtg ggt agt        672
Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser           
    210                 215                 220                           

tcc tca gga aat tgg cat tgc gat tcc aca tgg ctg ggc gac aga gtc        720
Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val           
225                 230                 235                 240           

atc acc acc agc acc cgc acc tgg gcc ctg ccc acc tac aac aac cac        768
Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His           
                245                 250                 255               

ctc tac aag caa atc tcc aac ggg acc tcg gga gga agc acc aac gac        816
Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp           
            260                 265                 270                   

aac acc tac ttc ggc tac agc acc ccc tgg ggg tat ttt gac ttc aac        864
Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn           
        275                 280                 285                       

aga ttc cac tgc cac ttt tca cca cgt gac tgg cag cga ctc atc aac        912
Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn           
    290                 295                 300                           

aac aac tgg gga ttc cgg ccc aag agg ctc aac ttc aag ctc ttc aac        960
Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn           
305                 310                 315                 320           

atc caa gtc aag gag gtc acg cag aat gaa ggc acc aag acc atc gcc       1008
Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala           
                325                 330                 335               

aat aac ctt acc agc acg att cag gtc ttt acg gac tcg gaa tac cag       1056
Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln           
            340                 345                 350                   

ctc ccg tac gtg ctc ggc tcg gcg cac cag ggc tgc ctg cct ccg ttc       1104
Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe           
        355                 360                 365                       

ccg gcg gac gtc ttc atg att cct cag tac ggg tac ctg act ctg aac       1152
Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn           
    370                 375                 380                           

aat ggc agt cag gct gtg ggc cgg tcg tcc ttc tac tgc ctg gag tac       1200
Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr           
385                 390                 395                 400           

ttt cct tct caa atg ctg aga acg ggc aac aac ttt gaa ttc agc tac       1248
Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr           
                405                 410                 415               

aac ttc gag gac gtg ccc ttc cac agc agc tac gcg cac agc cag agc       1296
Asn Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser           
            420                 425                 430                   

ctg gac cgg ctg atg aac cct ctc atc gac cag tac ttg tac tac ctg       1344
Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu           
        435                 440                 445                       

tcc aag act att aac ggt tct gga cag aat caa caa acg cta aaa ttc       1392
Ser Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe           
    450                 455                 460                           

agt gtg gcc gga ccc agc aac atg gct gtc cag gga aga aac tac ata       1440
Ser Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile           
465                 470                 475                 480           

cct gga ccc agc tac cga caa caa cgt gtc tca acc act gtg act caa       1488
Pro Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln           
                485                 490                 495               

aac aac aac agc gaa ttt gct tgg cct gga gct tct tct tgg gct ctc       1536
Asn Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu           
            500                 505                 510                   

aat gga cgt aat agc ttg atg aat cct gga cct gct atg gcc agc cac       1584
Asn Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His           
        515                 520                 525                       

aaa gaa gga gag gac cgt ttc ttt cct ttg tct gga tct tta att ttt       1632
Lys Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe           
    530                 535                 540                           

ggc aaa caa gga act gga aga gac aac gtg gat gcg gac aaa gtc atg       1680
Gly Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met           
545                 550                 555                 560           

ata acc aac gaa gaa gaa att aaa act act aac ccg gta gca acg gag       1728
Ile Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu           
                565                 570                 575               

tcc tat gga caa gtg gcc aca aac cac cag agt gcc caa gca cag gcg       1776
Ser Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala           
            580                 585                 590                   

cag acc ggc tgg gtt caa aac caa gga ata ctt ccg ggt atg gtt tgg       1824
Gln Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp           
        595                 600                 605                       

cag gac cgg gac gtg tac ctg cag ggt ccc atc tgg gcc aag att cct       1872
Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro           
    610                 615                 620                           

cat acg gac ggc aac ttt cat ccc tcg ccg ctg atg gga ggc ttt gga       1920
His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly           
625                 630                 635                 640           

ctg aag cat ccg cct cct cag atc ctg att aaa aac aca cct gtt ccc       1968
Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro           
                645                 650                 655               

gcg gat cct ccg acc acc ttc aat cag gcc aag ctg gct tct ttc atc       2016
Ala Asp Pro Pro Thr Thr Phe Asn Gln Ala Lys Leu Ala Ser Phe Ile           
            660                 665                 670                   

acg cag tac agt acc ggc cag gtc agc gtg gag atc gag tgg gag ctg       2064
Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu           
        675                 680                 685                       

cag aag gag aac agc aaa cgc tgg aac cca gag att cag tac act tcc       2112
Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser           
    690                 695                 700                           

aac tac tac aaa tct aca aat gtg gac ttt gct gtc aat act gag ggt       2160
Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly           
705                 710                 715                 720           

act tat tcc gag cct cgc ccc att ggc acc cgt tac ctc acc cgt aat       2208
Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn           
                725                 730                 735               

ctg taa                                                               2214
Leu                                                                       
                                                                          


<210>  8
<211>  737
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Asp Leu Lys Pro Gly Ala Pro Lys Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln Lys Gln Asp Asn Gly Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Gln Ala Gly Asp Asn Pro Tyr Leu Arg Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Gln Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Ser Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Pro Ser Pro Gln Arg Ser Pro Asp Ser Ser Thr Gly Ile 
145                 150                 155                 160 


Gly Lys Lys Gly Gln Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln 
                165                 170                 175     


Thr Gly Asp Ser Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro 
            180                 185                 190         


Pro Ala Gly Pro Ser Gly Leu Gly Ser Gly Thr Met Ala Ala Gly Gly 
        195                 200                 205             


Gly Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser 
    210                 215                 220                 


Ser Ser Gly Asn Trp His Cys Asp Ser Thr Trp Leu Gly Asp Arg Val 
225                 230                 235                 240 


Ile Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His 
                245                 250                 255     


Leu Tyr Lys Gln Ile Ser Asn Gly Thr Ser Gly Gly Ser Thr Asn Asp 
            260                 265                 270         


Asn Thr Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn 
        275                 280                 285             


Arg Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn 
    290                 295                 300                 


Asn Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn 
305                 310                 315                 320 


Ile Gln Val Lys Glu Val Thr Gln Asn Glu Gly Thr Lys Thr Ile Ala 
                325                 330                 335     


Asn Asn Leu Thr Ser Thr Ile Gln Val Phe Thr Asp Ser Glu Tyr Gln 
            340                 345                 350         


Leu Pro Tyr Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe 
        355                 360                 365             


Pro Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn 
    370                 375                 380                 


Asn Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr 
385                 390                 395                 400 


Phe Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Glu Phe Ser Tyr 
                405                 410                 415     


Asn Phe Glu Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser 
            420                 425                 430         


Leu Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu 
        435                 440                 445             


Ser Lys Thr Ile Asn Gly Ser Gly Gln Asn Gln Gln Thr Leu Lys Phe 
    450                 455                 460                 


Ser Val Ala Gly Pro Ser Asn Met Ala Val Gln Gly Arg Asn Tyr Ile 
465                 470                 475                 480 


Pro Gly Pro Ser Tyr Arg Gln Gln Arg Val Ser Thr Thr Val Thr Gln 
                485                 490                 495     


Asn Asn Asn Ser Glu Phe Ala Trp Pro Gly Ala Ser Ser Trp Ala Leu 
            500                 505                 510         


Asn Gly Arg Asn Ser Leu Met Asn Pro Gly Pro Ala Met Ala Ser His 
        515                 520                 525             


Lys Glu Gly Glu Asp Arg Phe Phe Pro Leu Ser Gly Ser Leu Ile Phe 
    530                 535                 540                 


Gly Lys Gln Gly Thr Gly Arg Asp Asn Val Asp Ala Asp Lys Val Met 
545                 550                 555                 560 


Ile Thr Asn Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu 
                565                 570                 575     


Ser Tyr Gly Gln Val Ala Thr Asn His Gln Ser Ala Gln Ala Gln Ala 
            580                 585                 590         


Gln Thr Gly Trp Val Gln Asn Gln Gly Ile Leu Pro Gly Met Val Trp 
        595                 600                 605             


Gln Asp Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro 
    610                 615                 620                 


His Thr Asp Gly Asn Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly 
625                 630                 635                 640 


Leu Lys His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro 
                645                 650                 655     


Ala Asp Pro Pro Thr Thr Phe Asn Gln Ala Lys Leu Ala Ser Phe Ile 
            660                 665                 670         


Thr Gln Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu 
        675                 680                 685             


Gln Lys Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser 
    690                 695                 700                 


Asn Tyr Tyr Lys Ser Thr Asn Val Asp Phe Ala Val Asn Thr Glu Gly 
705                 710                 715                 720 


Thr Tyr Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn 
                725                 730                 735     


Leu 
    


<210>  9
<211>  746
<212>  PRT
<213>  artificial sequence

<220>
<223>  peptide-modified AAV9-rh74 hybrid capsid

<400>  9

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe 
    450                 455                 460                 


Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu 
465                 470                 475                 480 


Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln 
                485                 490                 495     


Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His Leu 
            500                 505                 510         


Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr His 
        515                 520                 525             


Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe 
    530                 535                 540                 


Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met 
545                 550                 555                 560 


Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu 
                565                 570                 575     


Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gly Gln Ser Gly Arg Gly 
            580                 585                 590         


Asp Leu Gly Leu Ser Ala Gln Ala Ala Ile Val Gly Ala Val Asn Ser 
        595                 600                 605             


Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu 
    610                 615                 620                 


Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His 
625                 630                 635                 640 


Pro Ser Pro Leu Met Gly Gly Phe Gly Met Lys His Pro Pro Pro Gln 
                645                 650                 655     


Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asp Pro Pro Thr Ala Phe 
            660                 665                 670         


Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln 
        675                 680                 685             


Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg 
    690                 695                 700                 


Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Tyr Lys Ser Asn Asn 
705                 710                 715                 720 


Val Glu Phe Ala Val Asn Thr Glu Gly Val Tyr Ser Glu Pro Arg Pro 
                725                 730                 735     


Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
            740                 745     


<210>  10
<211>  2241
<212>  DNA
<213>  artificial sequence

<220>
<223>  synthetic polynucleotide encoding peptide-modified  AAV9-rh74 
       hybrid capsid


<220>
<221>  CDS
<222>  (1)..(2241)

<400>  10
atg gct gcc gat ggt tat ctt cca gat tgg ctc gag gac aac ctt agt         48
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser           
1               5                   10                  15                

gaa gga att cgc gag tgg tgg gct ttg aaa cct gga gcc cct caa ccc         96
Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro           
            20                  25                  30                    

aag gca aat caa caa cat caa gac aac gct cga ggt ctt gtg ctt ccg        144
Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro           
        35                  40                  45                        

ggt tac aaa tac ctt gga ccc ggc aac gga ctc gac aag ggg gag ccg        192
Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro           
    50                  55                  60                            

gtc aac gca gca gac gcg gcg gcc ctc gag cac gac aag gcc tac gac        240
Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp           
65                  70                  75                  80            

cag cag ctc aag gcc gga gac aac ccg tac ctc aag tac aac cac gcc        288
Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala           
                85                  90                  95                

gac gcc gag ttc cag gag cgg ctc aaa gaa gat acg tct ttt ggg ggc        336
Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly           
            100                 105                 110                   

aac ctc ggg cga gca gtc ttc cag gcc aaa aag agg ctt ctt gaa cct        384
Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro           
        115                 120                 125                       

ctt ggt ctg gtt gag gaa gcg gct aag acg gct cct gga aag aag agg        432
Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg           
    130                 135                 140                           

cct gta gag cag tct cct cag gaa ccg gac tcc tcc gcg ggt att ggc        480
Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly           
145                 150                 155                 160           

aaa tcg ggt gca cag ccc gct aaa aag aga ctc aat ttc ggt cag act        528
Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr           
                165                 170                 175               

ggc gac aca gag tca gtc cca gac cct caa cca atc gga gaa cct ccc        576
Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro           
            180                 185                 190                   

gca gcc ccc tca ggt gtg gga tct ctt aca atg gct tca ggt ggt ggc        624
Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly           
        195                 200                 205                       

gca cca gtg gca gac aat aac gaa ggt gcc gat gga gtg ggt agt tcc        672
Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser           
    210                 215                 220                           

tcg gga aat tgg cat tgc gat tcc caa tgg ctg ggg gac aga gtc atc        720
Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile           
225                 230                 235                 240           

acc acc agc acc cga acc tgg gcc ctg ccc acc tac aac aat cac ctc        768
Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu           
                245                 250                 255               

tac aag caa atc tcc aac agc aca tct gga gga tct tca aat gac aac        816
Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn           
            260                 265                 270                   

gcc tac ttc ggc tac agc acc ccc tgg ggg tat ttt gac ttc aac aga        864
Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg           
        275                 280                 285                       

ttc cac tgc cac ttc tca cca cgt gac tgg cag cga ctc atc aac aac        912
Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn           
    290                 295                 300                           

aac tgg gga ttc cgg cct aag cga ctc aac ttc aag ctc ttc aac att        960
Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile           
305                 310                 315                 320           

cag gtc aaa gag gtt acg gac aac aat gga gtc aag acc atc gcc aat       1008
Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn           
                325                 330                 335               

aac ctt acc agc acg gtc cag gtc ttc acg gac tca gac tat cag ctc       1056
Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu           
            340                 345                 350                   

ccg tac gtg ctc ggg tcg gct cac gag ggc tgc ctc ccg ccg ttc cca       1104
Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro           
        355                 360                 365                       

gcg gac gtt ttc atg att cct cag tac ggg tat ctg acg ctt aat gat       1152
Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp           
    370                 375                 380                           

gga agc cag gcc gtg ggt cgt tcg tcc ttt tac tgc ctg gaa tat ttc       1200
Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe           
385                 390                 395                 400           

ccg tcg caa atg cta aga acg ggt aac aac ttc cag ttc agc tac gag       1248
Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu           
                405                 410                 415               

ttt gag aac gta cct ttc cat agc agc tac gct cac agc caa agc ctg       1296
Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu           
            420                 425                 430                   

gac cga cta atg aat cca ctc atc gac caa tac ttg tac tat ctc tca       1344
Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser           
        435                 440                 445                       

cgg act caa agc acg ggc ggt act gca gga act cag cag ttg cta ttt       1392
Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe           
    450                 455                 460                           

tct cag gcc ggg cct aac aac atg tcg gct cag gcc aag aac tgg cta       1440
Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu           
465                 470                 475                 480           

ccc ggt ccc tgc tac cgg cag caa cgc gtc tcc acg aca ctg tcg cag       1488
Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln           
                485                 490                 495               

aac aac aac agc aac ttt gcc tgg acg ggt gcc acc aag tat cat ctg       1536
Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His Leu           
            500                 505                 510                   

aat ggc aga gac tct ctg gtg aat cct ggc gtt gcc atg gct acc cac       1584
Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr His           
        515                 520                 525                       

aag gac gac gaa gag cga ttt ttt cca tcc agc gga gtc tta atg ttt       1632
Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe           
    530                 535                 540                           

ggg aaa cag gga gct gga aaa gac aac gtg gac tat agc agc gtg atg       1680
Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met           
545                 550                 555                 560           

cta acc agc gag gaa gaa ata aag acc acc aac cca gtg gcc aca gaa       1728
Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu           
                565                 570                 575               

cag tac ggc gtg gtg gcc gat aac ctg caa ggc cag agt ggc cgc ggc       1776
Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gly Gln Ser Gly Arg Gly           
            580                 585                 590                   

gat ctg ggc ctg agc gcc cag gcg gcc att gta ggg gcc gtc aat agt       1824
Asp Leu Gly Leu Ser Ala Gln Ala Ala Ile Val Gly Ala Val Asn Ser           
        595                 600                 605                       

caa gga gcc tta cct ggc atg gtg tgg cag aac aga gat gtg tac ctg       1872
Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu           
    610                 615                 620                           

caa gga ccc att tgg gcc aaa att cct cac acg gac ggc aac ttt cac       1920
Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His           
625                 630                 635                 640           

cct tct ccg ctg atg gga ggg ttt gga atg aag cac ccg cct cct cag       1968
Pro Ser Pro Leu Met Gly Gly Phe Gly Met Lys His Pro Pro Pro Gln           
                645                 650                 655               

atc ctc atc aaa aac aca cct gta cct gcg gat cct cca acg gcc ttc       2016
Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asp Pro Pro Thr Ala Phe           
            660                 665                 670                   

aac aag gac aag ctg aac tct ttc atc acc cag tat tct act ggc caa       2064
Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln           
        675                 680                 685                       

gtc agc gtg gag atc gag tgg gag ctg cag aag gaa aac agc aag cgc       2112
Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg           
    690                 695                 700                           

tgg aac ccg gag atc cag tac act tcc aac tat tac aag tct aat aat       2160
Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Tyr Lys Ser Asn Asn           
705                 710                 715                 720           

gtt gaa ttt gct gtt aat act gaa ggt gta tat agt gaa ccc cgc ccc       2208
Val Glu Phe Ala Val Asn Thr Glu Gly Val Tyr Ser Glu Pro Arg Pro           
                725                 730                 735               

att ggc acc aga tac ctg act cgt aat ctg taa                           2241
Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu                                   
            740                 745                                       


<210>  11
<211>  746
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  11

Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Asn Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Glu Trp Trp Ala Leu Lys Pro Gly Ala Pro Gln Pro 
            20                  25                  30          


Lys Ala Asn Gln Gln His Gln Asp Asn Ala Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Gly Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Ala Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Gln Gln Leu Lys Ala Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Leu Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Ala Ala Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu Gln Ser Pro Gln Glu Pro Asp Ser Ser Ala Gly Ile Gly 
145                 150                 155                 160 


Lys Ser Gly Ala Gln Pro Ala Lys Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Thr Glu Ser Val Pro Asp Pro Gln Pro Ile Gly Glu Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Val Gly Ser Leu Thr Met Ala Ser Gly Gly Gly 
        195                 200                 205             


Ala Pro Val Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Ser Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Gln Trp Leu Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Asn Ser Thr Ser Gly Gly Ser Ser Asn Asp Asn 
            260                 265                 270         


Ala Tyr Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg 
        275                 280                 285             


Phe His Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn 
    290                 295                 300                 


Asn Trp Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile 
305                 310                 315                 320 


Gln Val Lys Glu Val Thr Asp Asn Asn Gly Val Lys Thr Ile Ala Asn 
                325                 330                 335     


Asn Leu Thr Ser Thr Val Gln Val Phe Thr Asp Ser Asp Tyr Gln Leu 
            340                 345                 350         


Pro Tyr Val Leu Gly Ser Ala His Glu Gly Cys Leu Pro Pro Phe Pro 
        355                 360                 365             


Ala Asp Val Phe Met Ile Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asp 
    370                 375                 380                 


Gly Ser Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe 
385                 390                 395                 400 


Pro Ser Gln Met Leu Arg Thr Gly Asn Asn Phe Gln Phe Ser Tyr Glu 
                405                 410                 415     


Phe Glu Asn Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu 
            420                 425                 430         


Asp Arg Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser 
        435                 440                 445             


Arg Thr Gln Ser Thr Gly Gly Thr Ala Gly Thr Gln Gln Leu Leu Phe 
    450                 455                 460                 


Ser Gln Ala Gly Pro Asn Asn Met Ser Ala Gln Ala Lys Asn Trp Leu 
465                 470                 475                 480 


Pro Gly Pro Cys Tyr Arg Gln Gln Arg Val Ser Thr Thr Leu Ser Gln 
                485                 490                 495     


Asn Asn Asn Ser Asn Phe Ala Trp Thr Gly Ala Thr Lys Tyr His Leu 
            500                 505                 510         


Asn Gly Arg Asp Ser Leu Val Asn Pro Gly Val Ala Met Ala Thr His 
        515                 520                 525             


Lys Asp Asp Glu Glu Arg Phe Phe Pro Ser Ser Gly Val Leu Met Phe 
    530                 535                 540                 


Gly Lys Gln Gly Ala Gly Lys Asp Asn Val Asp Tyr Ser Ser Val Met 
545                 550                 555                 560 


Leu Thr Ser Glu Glu Glu Ile Lys Thr Thr Asn Pro Val Ala Thr Glu 
                565                 570                 575     


Gln Tyr Gly Val Val Ala Asp Asn Leu Gln Gly Gln Ser Gly Arg Gly 
            580                 585                 590         


Asp Leu Gly Leu Ser Ala Gln Ala Ala Ile Val Gly Ala Val Asn Ser 
        595                 600                 605             


Gln Gly Ala Leu Pro Gly Met Val Trp Gln Asn Arg Asp Val Tyr Leu 
    610                 615                 620                 


Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr Asp Gly Asn Phe His 
625                 630                 635                 640 


Pro Ser Pro Leu Met Gly Gly Phe Gly Met Lys His Pro Pro Pro Gln 
                645                 650                 655     


Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asp Pro Pro Thr Ala Phe 
            660                 665                 670         


Asn Lys Asp Lys Leu Asn Ser Phe Ile Thr Gln Tyr Ser Thr Gly Gln 
        675                 680                 685             


Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys Glu Asn Ser Lys Arg 
    690                 695                 700                 


Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr Tyr Lys Ser Asn Asn 
705                 710                 715                 720 


Val Glu Phe Ala Val Asn Thr Glu Gly Val Tyr Ser Glu Pro Arg Pro 
                725                 730                 735     


Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
            740                 745     


<210>  12
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  12

Arg Gly Asp Leu Gly Leu Ser 
1               5           


<210>  13
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  13

Gly Gly Leu Ser Gly Val Ser 
1               5           


<210>  14
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  14

Gly Gly Leu Ser Gly Asp Ser 
1               5           


<210>  15
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  syntehtic peptide

<400>  15

Gly Ser Val Ser Gly Ser Ala 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  16

Glu Tyr Arg Asp Ser Ser Gly 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  17

Gln Met Ser Gly Gly Val Ala 
1               5           


<210>  18
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  18

Glu Ser Gly Leu Ser Gln Ser 
1               5           


<210>  19
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  19

Glu Ser Gly Ile Trp Val Ala 
1               5           


<210>  20
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  20

Glu Glu Pro Ala Leu Arg Ala 
1               5           


<210>  21
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  21

Ala Pro Thr Leu Gly Ser Pro 
1               5           


<210>  22
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  22

Asp Leu Gly Ser Ala Arg Ala 
1               5           


<210>  23
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  23

Asp Gly Leu Gly Arg Leu Val 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  24

Asp Leu Arg Gly Leu Ala Ser 
1               5           


<210>  25
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  25

Asp Arg Ser Pro Leu Ser Leu 
1               5           


<210>  26
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  26

Ala Ile Ser Gly Val Gly Ser 
1               5           


<210>  27
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  27

Asp Arg Ser Gly Val Gly Ser 
1               5           


<210>  28
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  28

Ser Ile Ser Gly Val Gly Ser 
1               5           


<210>  29
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  29

Ser Glu Gly Arg Ser Gly Val 
1               5           


<210>  30
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  30

Gly Glu Ala Arg Ser Arg Ala 
1               5           


<210>  31
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  31

Gly Glu Ala Arg Ile Ser Ala 
1               5           


<210>  32
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  32

Ser Gly Asn Ser Gly Ala Ala 
1               5           


<210>  33
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  33

Ser Ser Gly Ser Gly Gly Ala 
1               5           


<210>  34
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  34

Glu Ser Gly Ile Trp Val Ala 
1               5           


<210>  35
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  35

Gly Gln Ser Gly 
1               


<210>  36
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  36

Ala Gln Ala Ala 
1               


<210>  37
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  37

Lys Thr Ile Asn Gly Ser 
1               5       


<210>  38
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  38

Pro Gly Met Val Trp Gln Asp 
1               5           


<210>  39
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  39

Arg Thr Gln Ser Thr Gly 
1               5       


<210>  40
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  40

Pro Gly Met Val Trp Gln Asn 
1               5           


<210>  41
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  41

Gln Gln Asn Ala Ala Pro 
1               5       


<210>  42
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic peptide

<400>  42

Gly Gln Ser Gly Ala Gln Ala Ala 
1               5               


