                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  MANNANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

<130>  14589-WO-PCT

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  325
<212>  PRT
<213>  Paenibacillus sp


<220>
<221>  SIGNAL
<222>  (1)..(28)

<220>
<221>  mat_peptide
<222>  (29)..(357)

<400>  1

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
            -25                 -20                 -15         


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Met Ala Thr Gly 
        -10                 -5              -1  1               


Phe Tyr Val Ser Gly Asn Lys Leu Tyr Asp Ser Thr Gly Lys Pro Phe 
5                   10                  15                  20  


Val Met Arg Gly Val Asn His Gly His Ser Trp Phe Lys Asn Asp Leu 
                25                  30                  35      


Asn Thr Ala Ile Pro Ala Ile Ala Lys Thr Gly Ala Asn Thr Val Arg 
            40                  45                  50          


Ile Val Leu Ser Asn Gly Ser Leu Tyr Thr Lys Asp Asp Leu Asn Ala 
        55                  60                  65              


Val Lys Asn Ile Ile Asn Val Val Asn Gln Asn Lys Met Ile Ala Val 
    70                  75                  80                  


Leu Glu Val His Asp Ala Thr Gly Lys Asp Asp Tyr Asn Ser Leu Asp 
85                  90                  95                  100 


Ala Ala Val Asn Tyr Trp Ile Ser Ile Lys Glu Ala Leu Ile Gly Lys 
                105                 110                 115     


Glu Asp Arg Val Ile Val Asn Ile Ala Asn Glu Trp Tyr Gly Thr Trp 
            120                 125                 130         


Asn Gly Ser Ala Trp Ala Asp Gly Tyr Lys Lys Ala Ile Pro Lys Leu 
        135                 140                 145             


Arg Asn Ala Gly Ile Lys Asn Thr Leu Ile Val Asp Ala Ala Gly Trp 
    150                 155                 160                 


Gly Gln Phe Pro Gln Ser Ile Val Asp Tyr Gly Gln Ser Val Phe Ala 
165                 170                 175                 180 


Ala Asp Ser Gln Lys Asn Thr Val Phe Ser Ile His Met Tyr Glu Tyr 
                185                 190                 195     


Ala Gly Lys Asp Ala Ala Thr Val Lys Ala Asn Met Glu Asn Val Leu 
            200                 205                 210         


Asn Lys Gly Leu Ala Leu Ile Ile Gly Glu Phe Gly Gly Tyr His Thr 
        215                 220                 225             


Asn Gly Asp Val Asp Glu Tyr Ala Ile Met Arg Tyr Gly Gln Glu Lys 
    230                 235                 240                 


Gly Val Gly Trp Leu Ala Trp Ser Trp Tyr Gly Asn Ser Ser Gly Leu 
245                 250                 255                 260 


Asn Tyr Leu Asp Met Ala Thr Gly Pro Asn Gly Ser Leu Thr Ser Phe 
                265                 270                 275     


Gly Asn Thr Val Val Asn Asp Thr Tyr Gly Ile Lys Asn Thr Ser Gln 
            280                 285                 290         


Lys Ala Gly Ile Phe 
        295         


<210>  2
<211>  297
<212>  PRT
<213>  Paenibacillus sp

<400>  2

Met Ala Thr Gly Phe Tyr Val Ser Gly Asn Lys Leu Tyr Asp Ser Thr 
1               5                   10                  15      


Gly Lys Pro Phe Val Met Arg Gly Val Asn His Gly His Ser Trp Phe 
            20                  25                  30          


Lys Asn Asp Leu Asn Thr Ala Ile Pro Ala Ile Ala Lys Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asn Gly Ser Leu Tyr Thr Lys Asp 
    50                  55                  60                  


Asp Leu Asn Ala Val Lys Asn Ile Ile Asn Val Val Asn Gln Asn Lys 
65                  70                  75                  80  


Met Ile Ala Val Leu Glu Val His Asp Ala Thr Gly Lys Asp Asp Tyr 
                85                  90                  95      


Asn Ser Leu Asp Ala Ala Val Asn Tyr Trp Ile Ser Ile Lys Glu Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Arg Val Ile Val Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Tyr Gly Thr Trp Asn Gly Ser Ala Trp Ala Asp Gly Tyr Lys Lys Ala 
    130                 135                 140                 


Ile Pro Lys Leu Arg Asn Ala Gly Ile Lys Asn Thr Leu Ile Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile Val Asp Tyr Gly Gln 
                165                 170                 175     


Ser Val Phe Ala Ala Asp Ser Gln Lys Asn Thr Val Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Lys Asp Ala Ala Thr Val Lys Ala Asn Met 
        195                 200                 205             


Glu Asn Val Leu Asn Lys Gly Leu Ala Leu Ile Ile Gly Glu Phe Gly 
    210                 215                 220                 


Gly Tyr His Thr Asn Gly Asp Val Asp Glu Tyr Ala Ile Met Arg Tyr 
225                 230                 235                 240 


Gly Gln Glu Lys Gly Val Gly Trp Leu Ala Trp Ser Trp Tyr Gly Asn 
                245                 250                 255     


Ser Ser Gly Leu Asn Tyr Leu Asp Met Ala Thr Gly Pro Asn Gly Ser 
            260                 265                 270         


Leu Thr Ser Phe Gly Asn Thr Val Val Asn Asp Thr Tyr Gly Ile Lys 
        275                 280                 285             


Asn Thr Ser Gln Lys Ala Gly Ile Phe 
    290                 295         


<210>  3
<211>  326
<212>  PRT
<213>  Bacillus bogoriensis


<220>
<221>  SIGNAL
<222>  (1)..(28)

<220>
<221>  mat_peptide
<222>  (29)..(408)

<400>  3

Met Lys Gln Gln Lys Arg Leu Tyr Ala Arg Leu Leu Thr Leu Leu Phe 
            -25                 -20                 -15         


Ala Leu Ile Phe Leu Leu Pro His Ser Ala Ala Ala Ala Asn Ser Gly 
        -10                 -5              -1  1               


Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn Gly Asn Pro Phe 
5                   10                  15                  20  


Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr Lys Asp Gln Ala 
                25                  30                  35      


Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala Asn Thr Val Arg 
            40                  45                  50          


Ile Val Leu Ser Asp Gly Gly Gln Trp Thr Lys Asp Asp Ile His Thr 
        55                  60                  65              


Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His Leu Val Ala Val 
    70                  75                  80                  


Leu Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile Ala Ser Leu Asn 
85                  90                  95                  100 


Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala Leu Ile Gly Lys 
                105                 110                 115     


Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp Phe Gly Ser Trp 
            120                 125                 130         


Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gln Ala Ile Pro Arg Leu 
        135                 140                 145             


Arg Asn Ala Gly Leu Asn His Thr Leu Met Val Asp Ala Ala Gly Trp 
    150                 155                 160                 


Gly Gln Phe Pro Gln Ser Ile His Asp Tyr Gly Arg Glu Val Phe Asn 
165                 170                 175                 180 


Ala Asp Pro Gln Arg Asn Thr Met Phe Ser Ile His Met Tyr Glu Tyr 
                185                 190                 195     


Ala Gly Gly Asn Ala Ser Gln Val Arg Thr Asn Ile Asp Arg Val Leu 
            200                 205                 210         


Asn Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly His Arg His Thr 
        215                 220                 225             


Asn Gly Asp Val Asp Glu Ala Thr Ile Met Ser Tyr Ser Glu Gln Arg 
    230                 235                 240                 


Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn Gly Pro Glu Trp 
245                 250                 255                 260 


Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn Asn Leu Thr Ala 
                265                 270                 275     


Trp Gly Asn Thr Ile Val Asn Gly Pro Tyr Gly Leu Arg Glu Thr Ser 
            280                 285                 290         


Arg Leu Ser Thr Val Phe 
        295             


<210>  4
<211>  298
<212>  PRT
<213>  Bacillus bogoriensis

<400>  4

Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn 
1               5                   10                  15      


Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr 
            20                  25                  30          


Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp Thr Lys Asp 
    50                  55                  60                  


Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His 
65                  70                  75                  80  


Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile 
                85                  90                  95      


Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gln Ala 
    130                 135                 140                 


Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu Met Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp Tyr Gly Arg 
                165                 170                 175     


Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg Thr Asn Ile 
        195                 200                 205             


Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly 
    210                 215                 220                 


His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile Met Ser Tyr 
225                 230                 235                 240 


Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn 
                245                 250                 255     


Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn 
            260                 265                 270         


Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro Tyr Gly Leu 
        275                 280                 285             


Arg Glu Thr Ser Arg Leu Ser Thr Val Phe 
    290                 295             


<210>  5
<211>  269
<212>  PRT
<213>  Bacillus subtilis

<400>  5

Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


