﻿                         序列表

<110>  成都优娃生物科技有限公司

<120>  应用慢病毒载体EF1α启动子优化表达ABCD1基因治疗肾上腺脑白质营养不良症

<160>  4

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  2238
<212>  DNA
<213>  人类(human being)

<400>  1
atgccggtgc tctccaggcc ccggccctgg cgggggaaca cgctgaagcg cacggccgtg        60
ctcctggccc tcgcggccta tggagcccac aaagtctacc ccttggtgcg ccagtgcctg       120
gccccggcca ggggtcttca ggcgcccgcc ggggagccca cgcaggaggc ctccggggtc       180
gcggcggcca aagctggcat gaaccgggta ttcctgcagc ggctcctgtg gctcctgcgg       240
ctgctgttcc cccgggtcct gtgccgggag acggggctgc tggccctgca ctcggccgcc       300
ttggtgagcc gcaccttcct gtcggtgtat gtggcccgcc tggacggaag gctggcccgc       360
tgcatcgtcc gcaaggaccc gcgggctttt ggctggcagc tgctgcagtg gctcctcatc       420
gccctccctg ctaccttcgt caacagtgcc atccgttacc tggagggcca actggccctg       480
tcgttccgca gccgtctggt ggcccacgcc taccgcctct acttctccca gcagacctac       540
taccgggtca gcaacatgga cgggcggctt cgcaaccctg accagtctct gacggaggac       600
gtggtggcct ttgcggcctc tgtggcccac ctctactcca acctgaccaa gccactcctg       660
gacgtggctg tgacttccta caccctgctt cgggcggccc gctcccgtgg agccggcaca       720
gcctggccct cggccatcgc cggcctcgtg gtgttcctca cggccaacgt gctgcgggcc       780
ttctcgccca agttcgggga gctggtggca gaggaggcgc ggcggaaggg ggagctgcgc       840
tacatgcact cgcgtgtggt ggccaactcg gaggagatcg ccttctatgg gggccatgag       900
gtggagctgg ccctgctaca gcgctcctac caggacctgg cctcgcagat caacctcatc       960
cttctggaac gcctgtggta tgttatgctg gagcagttcc tcatgaagta tgtgtggagc      1020
gcctcgggcc tgctcatggt ggctgtcccc atcatcactg ccactggcta ctcagagtca      1080
gatgcagagg ccgtgaagaa ggcagccttg gaaaagaagg aggaggagct ggtgagcgag      1140
cgcacagaag ccttcactat tgcccgcaac ctcctgacag cggctgcaga tgccattgag      1200
cggatcatgt cgtcgtacaa ggaggtgacg gagctggctg gctacacagc ccgggtgcac      1260
gagatgttcc aggtatttga agatgttcag cgctgtcact tcaagaggcc cagggagcta      1320
gaggacgctc aggcggggtc tgggaccata ggccggtctg gtgtccgtgt ggagggcccc      1380
ctgaagatcc gaggccaggt ggtggatgtg gaacagggga tcatctgcga gaacatcccc      1440
atcgtcacgc cctcaggaga ggtggtggtg gccagcctca acatcagggt ggaggaaggc      1500
atgcatctgc tcatcacagg ccccaatggc tgcggcaaga gctccctgtt ccggatcctg      1560
ggtgggctct ggcccacgta cggtggtgtg ctctacaagc ccccacccca gcgcatgttc      1620
tacatcccgc agaggcccta catgtctgtg ggctccctgc gtgaccaggt gatctacccg      1680
gactcagtgg aggacatgca aaggaagggc tactcggagc aggacctgga agccatcctg      1740
gacgtcgtgc acctgcacca catcctgcag cgggagggag gttgggaggc tatgtgtgac      1800
tggaaggacg tcctgtcggg tggcgagaag cagagaatcg gcatggcccg catgttctac      1860
cacaggccca agtacgccct cctggatgaa tgcaccagcg ccgtgagcat cgacgtggaa      1920
ggcaagatct tccaggcggc caaggacgcg ggcattgccc tgctctccat cacccaccgg      1980
ccctccctgt ggaaatacca cacacacttg ctacagttcg atggggaggg cggctggaag      2040
ttcgagaagc tggactcagc tgcccgcctg agcctgacgg aggagaagca gcggctggag      2100
cagcagctgg cgggcattcc caagatgcag cggcgcctcc aggagctctg ccagatcctg      2160
ggcgaggccg tggccccagc gcatgtgccg gcacctagcc cgcaaggccc tggtggcctc      2220
cagggtgcct ccacctga                                                    2238

<210>  2
<211>  745
<212>  PRT
<213>  人类(human being)

<400>  2
Met Pro Val Leu Ser Arg Pro Arg Pro Trp Arg Gly Asn Thr Leu Lys 
1               5                   10                  15      
Arg Thr Ala Val Leu Leu Ala Leu Ala Ala Tyr Gly Ala His Lys Val 
            20                  25                  30          
Tyr Pro Leu Val Arg Gln Cys Leu Ala Pro Ala Arg Gly Leu Gln Ala 
        35                  40                  45              
Pro Ala Gly Glu Pro Thr Gln Glu Ala Ser Gly Val Ala Ala Ala Lys 
    50                  55                  60                  
Ala Gly Met Asn Arg Val Phe Leu Gln Arg Leu Leu Trp Leu Leu Arg 
65                  70                  75                  80  
Leu Leu Phe Pro Arg Val Leu Cys Arg Glu Thr Gly Leu Leu Ala Leu 
                85                  90                  95      
His Ser Ala Ala Leu Val Ser Arg Thr Phe Leu Ser Val Tyr Val Ala 
            100                 105                 110         
Arg Leu Asp Gly Arg Leu Ala Arg Cys Ile Val Arg Lys Asp Pro Arg 
        115                 120                 125             
Ala Phe Gly Trp Gln Leu Leu Gln Trp Leu Leu Ile Ala Leu Pro Ala 
    130                 135                 140                 
Thr Phe Val Asn Ser Ala Ile Arg Tyr Leu Glu Gly Gln Leu Ala Leu 
145                 150                 155                 160 
Ser Phe Arg Ser Arg Leu Val Ala His Ala Tyr Arg Leu Tyr Phe Ser 
                165                 170                 175     
Gln Gln Thr Tyr Tyr Arg Val Ser Asn Met Asp Gly Arg Leu Arg Asn 
            180                 185                 190         
Pro Asp Gln Ser Leu Thr Glu Asp Val Val Ala Phe Ala Ala Ser Val 
        195                 200                 205             
Ala His Leu Tyr Ser Asn Leu Thr Lys Pro Leu Leu Asp Val Ala Val 
    210                 215                 220                 
Thr Ser Tyr Thr Leu Leu Arg Ala Ala Arg Ser Arg Gly Ala Gly Thr 
225                 230                 235                 240 
Ala Trp Pro Ser Ala Ile Ala Gly Leu Val Val Phe Leu Thr Ala Asn 
                245                 250                 255     
Val Leu Arg Ala Phe Ser Pro Lys Phe Gly Glu Leu Val Ala Glu Glu 
            260                 265                 270         
Ala Arg Arg Lys Gly Glu Leu Arg Tyr Met His Ser Arg Val Val Ala 
        275                 280                 285             
Asn Ser Glu Glu Ile Ala Phe Tyr Gly Gly His Glu Val Glu Leu Ala 
    290                 295                 300                 
Leu Leu Gln Arg Ser Tyr Gln Asp Leu Ala Ser Gln Ile Asn Leu Ile 
305                 310                 315                 320 
Leu Leu Glu Arg Leu Trp Tyr Val Met Leu Glu Gln Phe Leu Met Lys 
                325                 330                 335     
Tyr Val Trp Ser Ala Ser Gly Leu Leu Met Val Ala Val Pro Ile Ile 
            340                 345                 350         
Thr Ala Thr Gly Tyr Ser Glu Ser Asp Ala Glu Ala Val Lys Lys Ala 
        355                 360                 365             
Ala Leu Glu Lys Lys Glu Glu Glu Leu Val Ser Glu Arg Thr Glu Ala 
    370                 375                 380                 
Phe Thr Ile Ala Arg Asn Leu Leu Thr Ala Ala Ala Asp Ala Ile Glu 
385                 390                 395                 400 
Arg Ile Met Ser Ser Tyr Lys Glu Val Thr Glu Leu Ala Gly Tyr Thr 
                405                 410                 415     
Ala Arg Val His Glu Met Phe Gln Val Phe Glu Asp Val Gln Arg Cys 
            420                 425                 430         
His Phe Lys Arg Pro Arg Glu Leu Glu Asp Ala Gln Ala Gly Ser Gly 
        435                 440                 445             
Thr Ile Gly Arg Ser Gly Val Arg Val Glu Gly Pro Leu Lys Ile Arg 
    450                 455                 460                 
Gly Gln Val Val Asp Val Glu Gln Gly Ile Ile Cys Glu Asn Ile Pro 
465                 470                 475                 480 
Ile Val Thr Pro Ser Gly Glu Val Val Val Ala Ser Leu Asn Ile Arg 
                485                 490                 495     
Val Glu Glu Gly Met His Leu Leu Ile Thr Gly Pro Asn Gly Cys Gly 
            500                 505                 510         
Lys Ser Ser Leu Phe Arg Ile Leu Gly Gly Leu Trp Pro Thr Tyr Gly 
        515                 520                 525             
Gly Val Leu Tyr Lys Pro Pro Pro Gln Arg Met Phe Tyr Ile Pro Gln 
    530                 535                 540                 
Arg Pro Tyr Met Ser Val Gly Ser Leu Arg Asp Gln Val Ile Tyr Pro 
545                 550                 555                 560 
Asp Ser Val Glu Asp Met Gln Arg Lys Gly Tyr Ser Glu Gln Asp Leu 
                565                 570                 575     
Glu Ala Ile Leu Asp Val Val His Leu His His Ile Leu Gln Arg Glu 
            580                 585                 590         
Gly Gly Trp Glu Ala Met Cys Asp Trp Lys Asp Val Leu Ser Gly Gly 
        595                 600                 605             
Glu Lys Gln Arg Ile Gly Met Ala Arg Met Phe Tyr His Arg Pro Lys 
    610                 615                 620                 
Tyr Ala Leu Leu Asp Glu Cys Thr Ser Ala Val Ser Ile Asp Val Glu 
625                 630                 635                 640 
Gly Lys Ile Phe Gln Ala Ala Lys Asp Ala Gly Ile Ala Leu Leu Ser 
                645                 650                 655     
Ile Thr His Arg Pro Ser Leu Trp Lys Tyr His Thr His Leu Leu Gln 
            660                 665                 670         
Phe Asp Gly Glu Gly Gly Trp Lys Phe Glu Lys Leu Asp Ser Ala Ala 
        675                 680                 685             
Arg Leu Ser Leu Thr Glu Glu Lys Gln Arg Leu Glu Gln Gln Leu Ala 
    690                 695                 700                 
Gly Ile Pro Lys Met Gln Arg Arg Leu Gln Glu Leu Cys Gln Ile Leu 
705                 710                 715                 720 
Gly Glu Ala Val Ala Pro Ala His Val Pro Ala Pro Ser Pro Gln Gly 
                725                 730                 735     
Pro Gly Gly Leu Gln Gly Ala Ser Thr 
            740                 745 

<210>  3
<211>  1936
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
gctagcatgc ctaggtcgac caattctcat gtttgacagc ttatcatcga taagctttgg        60
agctaagcca gcaatggtag agggaagatt ctgcacgtcc cttccaggcg gcctccccgt       120
caccaccccc cccaacccgc cccgaccgga gctgagagta attcatacaa aaggactcgc       180
ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag aacccagaga       240
tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg gagaagagca       300
tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa       360
gttgggggga ggggtcggca attgaaccgg tgcctagaga aagtggcgcg gggtaaactg       420
ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat       480
aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt       540
aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc       600
ttgaattact tccacgcccc tggctgcagt acgtgattct tgatcccgag cttcgggttg       660
gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag       720
ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct       780
gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc       840
tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg       900
tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc       960
ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg      1020
ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc      1080
ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct      1140
caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa      1200
gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca      1260
ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt      1320
tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc      1380
acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca      1440
agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga aaactctaga      1500
gcggccgcgg aggccgaatt cccgggatat cgtcgacgcc aggctgcgga gcggacggac      1560
gcgcctggtg ccccggggag gggcgccacc gggggaggag gaggaggaga aggtggagag      1620
gaagagacgc cccctctgcc cgagacctct caaggccctg acctcagggg ccagggcact      1680
gacaggacag gagagccaag ttcctccact tgggctgccc gaagaggccg cgaccctgga      1740
gggccctgag cccaccgcac caggggcccc agcaccaccc cgggggccta aagcgacagt      1800
ctcaggggcc atcgcaaggt ttccagttgc ctagacaaca ggcccagggt cagagcaaca      1860
atccttccag ccacctgcct caactgctgc cccaggcacc agccccagtc cctacgcggc      1920
agccagccca ggtgac                                                      1936

<210>  4
<211>  4174
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
gctagcatgc ctaggtcgac caattctcat gtttgacagc ttatcatcga taagctttgg        60
agctaagcca gcaatggtag agggaagatt ctgcacgtcc cttccaggcg gcctccccgt       120
caccaccccc cccaacccgc cccgaccgga gctgagagta attcatacaa aaggactcgc       180
ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag aacccagaga       240
tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg gagaagagca       300
tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa       360
gttgggggga ggggtcggca attgaaccgg tgcctagaga aagtggcgcg gggtaaactg       420
ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat       480
aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt       540
aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc       600
ttgaattact tccacgcccc tggctgcagt acgtgattct tgatcccgag cttcgggttg       660
gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag       720
ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct       780
gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc       840
tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg       900
tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc       960
ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg      1020
ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc      1080
ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct      1140
caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa      1200
gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca      1260
ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt      1320
tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc      1380
acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca      1440
agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga aaactctaga      1500
gcggccgcgg aggccgaatt cccgggatat cgtcgacgcc aggctgcgga gcggacggac      1560
gcgcctggtg ccccggggag gggcgccacc gggggaggag gaggaggaga aggtggagag      1620
gaagagacgc cccctctgcc cgagacctct caaggccctg acctcagggg ccagggcact      1680
gacaggacag gagagccaag ttcctccact tgggctgccc gaagaggccg cgaccctgga      1740
gggccctgag cccaccgcac caggggcccc agcaccaccc cgggggccta aagcgacagt      1800
ctcaggggcc atcgcaaggt ttccagttgc ctagacaaca ggcccagggt cagagcaaca      1860
atccttccag ccacctgcct caactgctgc cccaggcacc agccccagtc cctacgcggc      1920
agccagccca ggtgacatgc cggtgctctc caggccccgg ccctggcggg ggaacacgct      1980
gaagcgcacg gccgtgctcc tggccctcgc ggcctatgga gcccacaaag tctacccctt      2040
ggtgcgccag tgcctggccc cggccagggg tcttcaggcg cccgccgggg agcccacgca      2100
ggaggcctcc ggggtcgcgg cggccaaagc tggcatgaac cgggtattcc tgcagcggct      2160
cctgtggctc ctgcggctgc tgttcccccg ggtcctgtgc cgggagacgg ggctgctggc      2220
cctgcactcg gccgccttgg tgagccgcac cttcctgtcg gtgtatgtgg cccgcctgga      2280
cggaaggctg gcccgctgca tcgtccgcaa ggacccgcgg gcttttggct ggcagctgct      2340
gcagtggctc ctcatcgccc tccctgctac cttcgtcaac agtgccatcc gttacctgga      2400
gggccaactg gccctgtcgt tccgcagccg tctggtggcc cacgcctacc gcctctactt      2460
ctcccagcag acctactacc gggtcagcaa catggacggg cggcttcgca accctgacca      2520
gtctctgacg gaggacgtgg tggcctttgc ggcctctgtg gcccacctct actccaacct      2580
gaccaagcca ctcctggacg tggctgtgac ttcctacacc ctgcttcggg cggcccgctc      2640
ccgtggagcc ggcacagcct ggccctcggc catcgccggc ctcgtggtgt tcctcacggc      2700
caacgtgctg cgggccttct cgcccaagtt cggggagctg gtggcagagg aggcgcggcg      2760
gaagggggag ctgcgctaca tgcactcgcg tgtggtggcc aactcggagg agatcgcctt      2820
ctatgggggc catgaggtgg agctggccct gctacagcgc tcctaccagg acctggcctc      2880
gcagatcaac ctcatccttc tggaacgcct gtggtatgtt atgctggagc agttcctcat      2940
gaagtatgtg tggagcgcct cgggcctgct catggtggct gtccccatca tcactgccac      3000
tggctactca gagtcagatg cagaggccgt gaagaaggca gccttggaaa agaaggagga      3060
ggagctggtg agcgagcgca cagaagcctt cactattgcc cgcaacctcc tgacagcggc      3120
tgcagatgcc attgagcgga tcatgtcgtc gtacaaggag gtgacggagc tggctggcta      3180
cacagcccgg gtgcacgaga tgttccaggt atttgaagat gttcagcgct gtcacttcaa      3240
gaggcccagg gagctagagg acgctcaggc ggggtctggg accataggcc ggtctggtgt      3300
ccgtgtggag ggccccctga agatccgagg ccaggtggtg gatgtggaac aggggatcat      3360
ctgcgagaac atccccatcg tcacgccctc aggagaggtg gtggtggcca gcctcaacat      3420
cagggtggag gaaggcatgc atctgctcat cacaggcccc aatggctgcg gcaagagctc      3480
cctgttccgg atcctgggtg ggctctggcc cacgtacggt ggtgtgctct acaagccccc      3540
accccagcgc atgttctaca tcccgcagag gccctacatg tctgtgggct ccctgcgtga      3600
ccaggtgatc tacccggact cagtggagga catgcaaagg aagggctact cggagcagga      3660
cctggaagcc atcctggacg tcgtgcacct gcaccacatc ctgcagcggg agggaggttg      3720
ggaggctatg tgtgactgga aggacgtcct gtcgggtggc gagaagcaga gaatcggcat      3780
ggcccgcatg ttctaccaca ggcccaagta cgccctcctg gatgaatgca ccagcgccgt      3840
gagcatcgac gtggaaggca agatcttcca ggcggccaag gacgcgggca ttgccctgct      3900
ctccatcacc caccggccct ccctgtggaa ataccacaca cacttgctac agttcgatgg      3960
ggagggcggc tggaagttcg agaagctgga ctcagctgcc cgcctgagcc tgacggagga      4020
gaagcagcgg ctggagcagc agctggcggg cattcccaag atgcagcggc gcctccagga      4080
gctctgccag atcctgggcg aggccgtggc cccagcgcat gtgccggcac ctagcccgca      4140
aggccctggt ggcctccagg gtgcctccac ctga                                  4174

