                               SEQUENCE LISTING

<110> SANGAMO THERAPEUTICS, INC.
      MEDICAL RESEARCH COUNSEL
 
<120> GENETIC MODIFICATION OF MITOCHONDRIAL GENOMES

<130> 8325-0171.40

<140> PCT/US2019/023359
<141> 2019-03-21

<150> 62/646,156
<151> 2018-03-21

<160> 53    

<170> PatentIn version 3.5

<210> 1
<211> 196
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      FokI cleavage half domain sequence

<400> 1
Gln Leu Val Lys Ser Glu Leu Glu Glu Lys Lys Ser Glu Leu Arg His 
1               5                   10                  15      


Lys Leu Lys Tyr Val Pro His Glu Tyr Ile Glu Leu Ile Glu Ile Ala 
            20                  25                  30          


Arg Asn Ser Thr Gln Asp Arg Ile Leu Glu Met Lys Val Met Glu Phe 
        35                  40                  45              


Phe Met Lys Val Tyr Gly Tyr Arg Gly Lys His Leu Gly Gly Ser Arg 
    50                  55                  60                  


Lys Pro Asp Gly Ala Ile Tyr Thr Val Gly Ser Pro Ile Asp Tyr Gly 
65                  70                  75                  80  


Val Ile Val Asp Thr Lys Ala Tyr Ser Gly Gly Tyr Asn Leu Pro Ile 
                85                  90                  95      


Gly Gln Ala Asp Glu Met Gln Arg Tyr Val Glu Glu Asn Gln Thr Arg 
            100                 105                 110         


Asn Lys His Ile Asn Pro Asn Glu Trp Trp Lys Val Tyr Pro Ser Ser 
        115                 120                 125             


Val Thr Glu Phe Lys Phe Leu Phe Val Ser Gly His Phe Lys Gly Asn 
    130                 135                 140                 


Tyr Lys Ala Gln Leu Thr Arg Leu Asn His Ile Thr Asn Cys Asn Gly 
145                 150                 155                 160 


Ala Val Leu Ser Val Glu Glu Leu Leu Ile Gly Gly Glu Met Ile Lys 
                165                 170                 175     


Ala Gly Thr Leu Thr Leu Glu Glu Val Arg Arg Lys Phe Asn Asn Gly 
            180                 185                 190         


Glu Ile Asn Phe 
        195     


<210> 2
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 2
Leu Pro His His Leu Glu Gln 
1               5           


<210> 3
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 3
Arg Asn Ala Ser Arg Thr Arg 
1               5           


<210> 4
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 4
Tyr Thr Tyr Ser Leu Ser Glu 
1               5           


<210> 5
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 5
Gln Ser Ala Asn Arg Thr Thr 
1               5           


<210> 6
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 6
His Arg Ser Ser Leu Arg Arg 
1               5           


<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 7
Gly Asn Thr Gly Leu Asn Cys 
1               5           


<210> 8
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 8
Asp Arg Ser Asn Leu Thr Arg 
1               5           


<210> 9
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Gln Ser Gly Ser Leu Thr Arg 
1               5           


<210> 10
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
His Lys Ser Ala Arg Ala Ala 
1               5           


<210> 11
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Arg Ser Asp His Leu Ser Ala 
1               5           


<210> 12
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 12
Gln His Gly Ala Leu Gln Thr 
1               5           


<210> 13
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Arg Ser Asp His Leu Ser Gln 
1               5           


<210> 14
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 14
Gln Ser Asn Gly Leu Thr Gln 
1               5           


<210> 15
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 15
Gln His Gly Ser Leu Ala Ser 
1               5           


<210> 16
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 16
Arg Ser Ala His Leu Ser Ala 
1               5           


<210> 17
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 17
Ser Ser Ser His Arg Cys Gln 
1               5           


<210> 18
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 18
Gln Arg Val Ala Leu Gln Ala 
1               5           


<210> 19
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Gln Ser Gly Ala Leu Ala Arg 
1               5           


<210> 20
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 20
Tyr Arg Trp Leu Arg Asn Ser 
1               5           


<210> 21
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Trp Tyr Thr Ala Arg Tyr Gln 
1               5           


<210> 22
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Gln Arg Thr His Leu Thr Gln 
1               5           


<210> 23
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 23
Asp Arg Ser Asn Leu Ser Arg 
1               5           


<210> 24
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 24
Gln Gln Ala Asn Arg Lys Lys 
1               5           


<210> 25
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Arg Pro Tyr Thr Leu Arg Leu 
1               5           


<210> 26
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 26
Gln Ser Gly His Leu Ala Arg 
1               5           


<210> 27
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Gln Ser Ser Asn Arg Gln Lys 
1               5           


<210> 28
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 28
Arg Ser Phe Ser Leu Gln Val 
1               5           


<210> 29
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 29
Arg Thr Tyr Ser Leu Ala Val 
1               5           


<210> 30
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 30
Arg Asn Phe Ser Leu Thr Met 
1               5           


<210> 31
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 31
Gln Trp Tyr Gly Arg Ser Asn 
1               5           


<210> 32
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 32
Gln Ser Ala Asn Arg Thr Lys 
1               5           


<210> 33
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 33
aagttaaact tgtgtgtttt cttagggc                                          28


<210> 34
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 34
tgataaggat tgtaagactt catcctac                                          28


<210> 35
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 35
gataaggatt gtaagacttc atcctaca                                          28


<210> 36
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 36
atactagtcc gcgagccttc aaag                                              24


<210> 37
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 37
gagggttccg atatctttgt gatt                                              24


<210> 38
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 38
aagtttaact tctgataagg                                                   20


<210> 39
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 39
tgctagccgc aggcattact                                                   20


<210> 40
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 40
cgggatcaaa gaaagttgtg ttt                                               23


<210> 41
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 41
gcctacactg gagtccgtgc tact                                              24


<210> 42
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 42
ctgaccacac acgagctggt agaa                                              24


<210> 43
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 43
taatacgact cactataggg agactaagga ctgtaagact tcatc                       45


<210> 44
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 44
gaggtcttag cttaattaaa g                                                 21


<210> 45
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 45
taatacgact cactataggg agacaagtct tagtagagat ttctc                       45


<210> 46
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 46
ggtcttaagg tgatattcat g                                                 21


<210> 47
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 47
aagcctacag cacccggtat tcccaggcgg tctcccatcc aagtactaac ca               52


<210> 48
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 48
Arg Ser Phe Thr Leu Met Gln 
1               5           


<210> 49
<211> 9
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      "LAGLIDADG" family peptide motif sequence

<400> 49
Leu Ala Gly Leu Ile Asp Ala Asp Gly 
1               5                   


<210> 50
<211> 47
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: wildtype mtDNA
      oligonucleotide

<400> 50
gaaaacacac aagtttaact tctgataagg actgtaagac ttcatcc                     47


<210> 51
<211> 47
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 51
gaaaacacac aagtttaact tctgataagg attgtaagac ttcatcc                     47


<210> 52
<211> 43
<212> DNA
<213> Mus sp.

<400> 52
aaacacacaa gtttaacttc tgataaggat tgtaagactt cat                         43


<210> 53
<211> 69
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 53
gaggucuuag cuuaauuaaa gcaauugauu ugcauucaau agauguagga ugaagucuua       60

caauccuua                                                               69


