﻿                         序列表

<110>  中国农业科学院饲料研究所

<120>   具有高比活及热稳定性的淀粉酶突变体及其基因和应用

<160>  6

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  485
<212>  PRT
<213>  解淀粉芽孢杆菌(Bacillus amyloliquefaciens)

<400>  1
Ala Ala Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Val Pro 
1               5                   10                  15      
Asn Asp Gly Gln Gln Trp Asn Arg Leu Arg Thr Asp Ala Pro Tyr Leu 
            20                  25                  30          
Ser Ser Val Gly Ile Thr Ala Val Trp Thr Pro Pro Ala Tyr Lys Gly 
        35                  40                  45              
Thr Ser Gln Ala Asp Val Gly Tyr Gly Pro Tyr Asp Leu Tyr Asp Leu 
    50                  55                  60                  
Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys 
65                  70                  75                  80  
Gly Glu Leu Lys Ser Ala Val Asn Thr Leu His Ser Asn Gly Ile Gln 
                85                  90                  95      
Val Tyr Gly Asp Val Val Met Asn His Lys Ala Gly Ala Asp Tyr Thr 
            100                 105                 110         
Glu Asn Val Thr Ala Val Glu Val Asn Pro Ser Asn Arg Asn Gln Glu 
        115                 120                 125             
Thr Ser Gly Glu Tyr Asn Ile Gln Ala Trp Thr Gly Phe Asn Phe Pro 
    130                 135                 140                 
Gly Arg Gly Thr Thr Tyr Ser Asn Phe Lys Trp Gln Trp Phe His Phe 
145                 150                 155                 160 
Asp Gly Thr Asp Trp Asp Gln Ser Arg Ser Leu Ser Arg Ile Phe Lys 
                165                 170                 175     
Phe Arg Gly Thr Gly Lys Ala Trp Asp Trp Glu Val Ser Ser Glu Asn 
            180                 185                 190         
Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Tyr Asp His Pro 
        195                 200                 205             
Asp Val Val Asn Glu Met Lys Lys Trp Gly Val Trp Tyr Ala Asn Glu 
    210                 215                 220                 
Val Gly Leu Asp Gly Tyr Arg Leu Asp Ala Val Lys His Ile Lys Phe 
225                 230                 235                 240 
Ser Phe Leu Lys Asp Trp Val Asp Asn Ala Arg Ala Ala Thr Gly Lys 
                245                 250                 255     
Glu Met Phe Thr Val Gly Glu Tyr Trp Gln Asn Asp Leu Gly Ala Leu 
            260                 265                 270         
Asn Asn Tyr Leu Ala Lys Val Asn Tyr Asn Gln Ser Leu Phe Asp Ala 
        275                 280                 285             
Pro Leu His Tyr Asn Phe Tyr Ala Ala Ser Thr Gly Gly Gly Tyr Tyr 
    290                 295                 300                 
Asp Met Arg Asn Ile Leu Asn Asn Thr Leu Val Ala Ser Asn Pro Thr 
305                 310                 315                 320 
Lys Ala Val Thr Leu Val Glu Asn His Asp Thr Gln Pro Gly Gln Ser 
                325                 330                 335     
Leu Glu Ser Thr Val Gln Pro Trp Phe Lys Pro Leu Ala Tyr Ala Phe 
            340                 345                 350         
Ile Leu Thr Arg Ser Gly Gly Tyr Pro Ser Val Phe Tyr Gly Asp Met 
        355                 360                 365             
Tyr Gly Thr Lys Gly Thr Thr Thr Arg Glu Ile Pro Ala Leu Lys Ser 
    370                 375                 380                 
Lys Ile Glu Pro Leu Leu Lys Ala Arg Lys Asp Tyr Ala Tyr Gly Thr 
385                 390                 395                 400 
Gln Arg Asp Tyr Ile Asp Asn Pro Asp Val Ile Gly Trp Thr Arg Glu 
                405                 410                 415     
Gly Asp Ser Thr Lys Ala Lys Ser Gly Leu Ala Thr Val Ile Thr Asp 
            420                 425                 430         
Gly Pro Gly Gly Ser Lys Arg Met Tyr Val Gly Thr Ser Asn Ala Gly 
        435                 440                 445             
Glu Ile Trp Tyr Asp Leu Thr Gly Asn Arg Thr Asp Lys Ile Thr Ile 
    450                 455                 460                 
Gly Ser Asp Gly Tyr Ala Thr Phe Pro Val Asn Gly Gly Ser Val Ser 
465                 470                 475                 480 
Val Trp Val Gln Gln 
                485 

<210>  2
<211>  483
<212>  PRT
<213>  解淀粉芽孢杆菌(Bacillus amyloliquefaciens)

<400>  2
Ala Ala Thr Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Val Pro 
1               5                   10                  15      
Asn Asp Gly Gln Gln Trp Asn Arg Leu Arg Thr Asp Ala Pro Tyr Leu 
            20                  25                  30          
Ala Glu His Gly Ile Thr Ala Val Trp Thr Pro Pro Ala Tyr Lys Gly 
        35                  40                  45              
Thr Ser Gln Ala Asp Val Gly Tyr Gly Pro Tyr Asp Leu Tyr Asp Leu 
    50                  55                  60                  
Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr Lys 
65                  70                  75                  80  
Gly Glu Leu Lys Ser Ala Val Asn Thr Leu His Ser Asn Gly Ile Gln 
                85                  90                  95      
Val Tyr Gly Asp Val Val Met Asn His Lys Ala Gly Ala Asp Tyr Thr 
            100                 105                 110         
Glu Asn Val Thr Ala Val Glu Val Asn Pro Ser Asn Arg Asn Gln Glu 
        115                 120                 125             
Thr Ser Gly Glu Tyr Asn Ile Gln Ala Trp Thr Gly Phe Asn Phe Pro 
    130                 135                 140                 
Gly Arg Gly Thr Thr Tyr Ser Asn Phe Lys Trp Gln Trp Phe His Phe 
145                 150                 155                 160 
Asp Gly Thr Asp Trp Asp Gln Ser Arg Ser Leu Ser Arg Ile Phe Lys 
                165                 170                 175     
Phe Thr Gly Lys Ala Trp Asp Trp Glu Val Ser Ser Glu Asn Gly Asn 
            180                 185                 190         
Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Tyr Asp His Pro Asp Val 
        195                 200                 205             
Val Asn Glu Met Lys Lys Trp Gly Val Trp Tyr Ala Asn Glu Val Gly 
    210                 215                 220                 
Leu Asp Gly Tyr Arg Leu Asp Ala Val Lys His Ile Lys Phe Ser Phe 
225                 230                 235                 240 
Leu Lys Asp Trp Val Asp Asn Ala Arg Ala Ala Thr Gly Lys Glu Met 
                245                 250                 255     
Phe Thr Val Gly Glu Tyr Trp Gln Asn Asp Leu Gly Ala Leu Asn Asn 
            260                 265                 270         
Tyr Leu Ala Lys Val Asn Tyr Asn Gln Ser Leu Phe Asp Ala Pro Leu 
        275                 280                 285             
His Tyr Asn Phe Tyr Ala Ala Ser Thr Gly Gly Gly Tyr Tyr Asp Met 
    290                 295                 300                 
Arg Asn Ile Leu Asn Asn Thr Leu Val Ala Ser Asn Pro Thr Lys Ala 
305                 310                 315                 320 
Val Thr Leu Val Glu Asn His Asp Thr Gln Pro Gly Gln Ser Leu Glu 
                325                 330                 335     
Ser Thr Val Gln Pro Trp Phe Lys Pro Leu Ala Tyr Ala Phe Ile Leu 
            340                 345                 350         
Thr Arg Ser Gly Gly Tyr Pro Ser Val Phe Tyr Gly Asp Met Tyr Gly 
        355                 360                 365             
Thr Lys Gly Thr Thr Thr Arg Glu Ile Pro Ala Leu Lys Ser Lys Ile 
    370                 375                 380                 
Glu Pro Leu Leu Lys Ala Arg Lys Asp Tyr Ala Tyr Gly Thr Gln Arg 
385                 390                 395                 400 
Asp Tyr Ile Asp Asn Pro Asp Val Ile Gly Trp Thr Arg Glu Gly Asp 
                405                 410                 415     
Ser Thr Lys Ala Lys Ser Gly Leu Ala Thr Val Ile Thr Asp Gly Pro 
            420                 425                 430         
Gly Gly Ser Lys Arg Met Tyr Val Gly Thr Ser Asn Ala Gly Glu Ile 
        435                 440                 445             
Trp Tyr Asp Leu Thr Gly Asn Arg Thr Asp Lys Ile Thr Ile Gly Ser 
    450                 455                 460                 
Asp Gly Tyr Ala Thr Phe Pro Val Asn Gly Gly Ser Val Ser Val Trp 
465                 470                 475                 480 
Val Gln Gln 
            

<210>  3
<211>  50
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
ttacaaaaac atcagccgta ggatccgccg caacgaacgg aacaatgatg                   50

<210>  4
<211>  54
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
gggacgtcga cttagtggtg gtggtggtgg tgctgctgaa cccacactga gacg              54

<210>  5
<211>  51
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
gggttcagca gcaccaccac caccaccact aagtcgacgt ccccggggca g                 51

<210>  6
<211>  50
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  6
attgttccgt tcgttgcggc ggatcctacg gctgatgttt ttgtaatcgg                   50

