                         SEQUENCE LISTING

<110>  CSL Limited
 
<120>  METHOD OF TREATING ASTHMA

<130>  527441PCT

<150>  AU 2018900961
<151>  2018-03-23

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  319
<212>  PRT
<213>  artificial

<220>
<223>  amino acids 25-335 of Homo sapiens G-CSFR (hG-CSFR) with a 
       C-terminal polyhistidine tag

<400>  1

Glu Cys Gly His Ile Ser Val Ser Ala Pro Ile Val His Leu Gly Asp 
1               5                   10                  15      


Pro Ile Thr Ala Ser Cys Ile Ile Lys Gln Asn Cys Ser His Leu Asp 
            20                  25                  30          


Pro Glu Pro Gln Ile Leu Trp Arg Leu Gly Ala Glu Leu Gln Pro Gly 
        35                  40                  45              


Gly Arg Gln Gln Arg Leu Ser Asp Gly Thr Gln Glu Ser Ile Ile Thr 
    50                  55                  60                  


Leu Pro His Leu Asn His Thr Gln Ala Phe Leu Ser Cys Ala Leu Asn 
65                  70                  75                  80  


Trp Gly Asn Ser Leu Gln Ile Leu Asp Gln Val Glu Leu Arg Ala Gly 
                85                  90                  95      


Tyr Pro Pro Ala Ile Pro His Asn Leu Ser Cys Leu Met Asn Leu Thr 
            100                 105                 110         


Thr Ser Ser Leu Ile Cys Gln Trp Glu Pro Gly Pro Glu Thr His Leu 
        115                 120                 125             


Pro Thr Ser Phe Thr Leu Lys Ser Phe Lys Ser Arg Gly Asn Cys Gln 
    130                 135                 140                 


Thr Gln Gly Asp Ser Ile Leu Asp Cys Val Pro Lys Asp Gly Gln Ser 
145                 150                 155                 160 


His Cys Ser Ile Pro Arg Lys His Leu Leu Leu Tyr Gln Asn Met Gly 
                165                 170                 175     


Ile Trp Val Gln Ala Glu Asn Ala Leu Gly Thr Ser Met Ser Pro Gln 
            180                 185                 190         


Leu Cys Leu Asp Pro Met Asp Val Val Lys Leu Glu Pro Pro Met Leu 
        195                 200                 205             


Arg Thr Met Asp Pro Ser Pro Glu Ala Ala Pro Pro Gln Ala Gly Cys 
    210                 215                 220                 


Leu Gln Leu Ser Trp Glu Pro Trp Gln Pro Gly Leu His Ile Asn Gln 
225                 230                 235                 240 


Lys Cys Glu Leu Arg His Lys Pro Gln Arg Gly Glu Ala Ser Trp Ala 
                245                 250                 255     


Leu Val Gly Pro Leu Pro Leu Glu Ala Leu Gln Tyr Glu Leu Cys Gly 
            260                 265                 270         


Leu Leu Pro Ala Thr Ala Tyr Thr Leu Gln Ile Arg Cys Ile Arg Trp 
        275                 280                 285             


Pro Leu Pro Gly His Trp Ser Asp Trp Ser Pro Ser Leu Glu Leu Arg 
    290                 295                 300                 


Thr Thr Glu Arg Ala Pro Thr His His His His His His His His 
305                 310                 315                 


<210>  2
<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  VH of C1.2

<400>  2

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Leu Tyr 
            20                  25                  30          


Trp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Met Leu Gly Glu Leu Gly Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  3
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  VL of C1.2

<400>  3

Asp Ile Gln Met Thr Gln Ser Pro Ser Ala Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Leu Gln Asn Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr His Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Asn Val Glu Ile Arg 
            100                 105         


<210>  4
<211>  118
<212>  PRT
<213>  artificial

<220>
<223>  VH of C1.2G

<400>  4

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Leu Tyr 
            20                  25                  30          


Trp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Gly Glu Leu Gly Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  5
<211>  107
<212>  PRT
<213>  artificial

<220>
<223>  VL  of C1.2G

<400>  5

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Leu Gln Asn Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  6
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  HCDR1 of C1.2

<400>  6

Leu Tyr Trp Met Gly 
1               5   


<210>  7
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 HCDR2

<400>  7

Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  8
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 HCDR3

<400>  8

Leu Gly Glu Leu Gly Trp Phe Asp Pro 
1               5                   


<210>  9
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 LCDR1

<400>  9

Arg Ala Ser Gln Gly Ile Ser Ser Tyr Leu Asn 
1               5                   10      


<210>  10
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 LCDR2

<400>  10

Ala Ser Asn Leu Gln Asn 
1               5       


<210>  11
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  C1.2 LCDR3

<400>  11

Gln Gln Ser Tyr Ser Thr Pro Leu Thr 
1               5                   


<210>  12
<211>  8
<212>  PRT
<213>  artificial

<220>
<223>  consensus sequence of HCDR3 of C1.2


<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is an amino acid selected from the group consisting of 
       tryptophan, glutamine, methionine, serine, phenylalanine, 
       glutamic acid and histidine

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X iis an amino acid selected from the group consisting of 
       phenylalanine, tyrosine, methionine, serine, glycine and 
       isoleucine

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  X is an amino acid selected from the group consisting of aspartic
       acid, methionine, glutamine, serine, leucine, valine, arginine 
       and histidine

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  X is an amino acid selected from the group consisting of proline 
       gltuamic acid, alanine, leucine, phenylalanine, tyronis, 
       threonine, asparagine, aspartic acid, serine , glycine, arginine,
       lysine

<400>  12

Leu Gly Glu Leu Xaa Xaa Xaa Xaa 
1               5               


<210>  13
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  consensus sequence of LCDR3 of C1.2


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  X is an amino acid selected from the group consisting of 
       glutamine, glutamic acid, histidine, alanine or serine

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is an amino acid selected from the group consisting of 
       glutamine, valine, phenylalanine, asparagine and glutamic acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  X ian amino acid selected from the group consisting of serine or 
       glycine

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  X is an amino acid selected from the group consisting of 
       tryptophan, methionine, phenylalanine, tyrosine, isoleucine and 
       leucine

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is an amino acid selected from the group consisting of glutamic
       acid, methionine, glutamine, tryptophan, serine, valine, 
       asparagine, glycine, alanine, arganine, histidine, tyrosine, 
       lysine or threonine

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is an amino acid selected from the group consisting of 
       tyrosine, methionine, isoleucine or threonine

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  X is an amino acid selected from the group consisting of proline,
       alanine, histidine, glycine and lysine

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  X is an amino acid selected from the group consisting of leucine,
       glutamine, methionine, alanine, phenylalanine, isoleucine, 
       lysine, histidine and glycine

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  X is an amino acid selected from the group consisting of 
       threonine, phenylalanine, tyrosine, methionine, lysine, serine, 
       histidine, proline, tryptophan, isoleucine, glutamine, glycine 
       and valine

<400>  13

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   


<210>  14
<211>  445
<212>  PRT
<213>  artificial

<220>
<223>  C1.2G heavy chain IgG4 with S241P mutation

<400>  14

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Leu Tyr 
            20                  25                  30          


Trp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Gly Glu Leu Gly Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
        435                 440                 445 


<210>  15
<211>  214
<212>  PRT
<213>  artificial

<220>
<223>  C1.2G with kappa light chain

<400>  15

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser Ser Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Ala Ser Asn Leu Gln Asn Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Tyr Ser Thr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  16
<211>  836
<212>  PRT
<213>  Homo sapiens

<400>  16

Met Ala Arg Leu Gly Asn Cys Ser Leu Thr Trp Ala Ala Leu Ile Ile 
1               5                   10                  15      


Leu Leu Leu Pro Gly Ser Leu Glu Glu Cys Gly His Ile Ser Val Ser 
            20                  25                  30          


Ala Pro Ile Val His Leu Gly Asp Pro Ile Thr Ala Ser Cys Ile Ile 
        35                  40                  45              


Lys Gln Asn Cys Ser His Leu Asp Pro Glu Pro Gln Ile Leu Trp Arg 
    50                  55                  60                  


Leu Gly Ala Glu Leu Gln Pro Gly Gly Arg Gln Gln Arg Leu Ser Asp 
65                  70                  75                  80  


Gly Thr Gln Glu Ser Ile Ile Thr Leu Pro His Leu Asn His Thr Gln 
                85                  90                  95      


Ala Phe Leu Ser Cys Cys Leu Asn Trp Gly Asn Ser Leu Gln Ile Leu 
            100                 105                 110         


Asp Gln Val Glu Leu Arg Ala Gly Tyr Pro Pro Ala Ile Pro His Asn 
        115                 120                 125             


Leu Ser Cys Leu Met Asn Leu Thr Thr Ser Ser Leu Ile Cys Gln Trp 
    130                 135                 140                 


Glu Pro Gly Pro Glu Thr His Leu Pro Thr Ser Phe Thr Leu Lys Ser 
145                 150                 155                 160 


Phe Lys Ser Arg Gly Asn Cys Gln Thr Gln Gly Asp Ser Ile Leu Asp 
                165                 170                 175     


Cys Val Pro Lys Asp Gly Gln Ser His Cys Cys Ile Pro Arg Lys His 
            180                 185                 190         


Leu Leu Leu Tyr Gln Asn Met Gly Ile Trp Val Gln Ala Glu Asn Ala 
        195                 200                 205             


Leu Gly Thr Ser Met Ser Pro Gln Leu Cys Leu Asp Pro Met Asp Val 
    210                 215                 220                 


Val Lys Leu Glu Pro Pro Met Leu Arg Thr Met Asp Pro Ser Pro Glu 
225                 230                 235                 240 


Ala Ala Pro Pro Gln Ala Gly Cys Leu Gln Leu Cys Trp Glu Pro Trp 
                245                 250                 255     


Gln Pro Gly Leu His Ile Asn Gln Lys Cys Glu Leu Arg His Lys Pro 
            260                 265                 270         


Gln Arg Gly Glu Ala Ser Trp Ala Leu Val Gly Pro Leu Pro Leu Glu 
        275                 280                 285             


Ala Leu Gln Tyr Glu Leu Cys Gly Leu Leu Pro Ala Thr Ala Tyr Thr 
    290                 295                 300                 


Leu Gln Ile Arg Cys Ile Arg Trp Pro Leu Pro Gly His Trp Ser Asp 
305                 310                 315                 320 


Trp Ser Pro Ser Leu Glu Leu Arg Thr Thr Glu Arg Ala Pro Thr Val 
                325                 330                 335     


Arg Leu Asp Thr Trp Trp Arg Gln Arg Gln Leu Asp Pro Arg Thr Val 
            340                 345                 350         


Gln Leu Phe Trp Lys Pro Val Pro Leu Glu Glu Asp Ser Gly Arg Ile 
        355                 360                 365             


Gln Gly Tyr Val Val Ser Trp Arg Pro Ser Gly Gln Ala Gly Ala Ile 
    370                 375                 380                 


Leu Pro Leu Cys Asn Thr Thr Glu Leu Ser Cys Thr Phe His Leu Pro 
385                 390                 395                 400 


Ser Glu Ala Gln Glu Val Ala Leu Val Ala Tyr Asn Ser Ala Gly Thr 
                405                 410                 415     


Ser Arg Pro Thr Pro Val Val Phe Ser Glu Ser Arg Gly Pro Ala Leu 
            420                 425                 430         


Thr Arg Leu His Ala Met Ala Arg Asp Pro His Ser Leu Trp Val Gly 
        435                 440                 445             


Trp Glu Pro Pro Asn Pro Trp Pro Gln Gly Tyr Val Ile Glu Trp Gly 
    450                 455                 460                 


Leu Gly Pro Pro Ser Ala Ser Asn Ser Asn Lys Thr Trp Arg Met Glu 
465                 470                 475                 480 


Gln Asn Gly Arg Ala Thr Gly Phe Leu Leu Lys Glu Asn Ile Arg Pro 
                485                 490                 495     


Phe Gln Leu Tyr Glu Ile Ile Val Thr Pro Leu Tyr Gln Asp Thr Met 
            500                 505                 510         


Gly Pro Ser Gln His Val Tyr Ala Tyr Ser Gln Glu Met Ala Pro Ser 
        515                 520                 525             


His Ala Pro Glu Leu His Leu Lys His Ile Gly Lys Thr Trp Ala Gln 
    530                 535                 540                 


Leu Glu Trp Val Pro Glu Pro Pro Glu Leu Gly Lys Ser Pro Leu Thr 
545                 550                 555                 560 


His Tyr Thr Ile Phe Trp Thr Asn Ala Gln Asn Gln Ser Phe Ser Ala 
                565                 570                 575     


Ile Leu Asn Ala Ser Ser Arg Gly Phe Val Leu His Gly Leu Glu Pro 
            580                 585                 590         


Ala Ser Leu Tyr His Ile His Leu Met Ala Ala Ser Gln Ala Gly Ala 
        595                 600                 605             


Thr Asn Ser Thr Val Leu Thr Leu Met Thr Leu Thr Pro Glu Gly Ser 
    610                 615                 620                 


Glu Leu His Ile Ile Leu Gly Leu Phe Gly Leu Leu Leu Leu Leu Thr 
625                 630                 635                 640 


Cys Leu Cys Gly Thr Ala Trp Leu Cys Cys Ser Pro Asn Arg Lys Asn 
                645                 650                 655     


Pro Leu Trp Pro Ser Val Pro Asp Pro Ala His Ser Ser Leu Gly Ser 
            660                 665                 670         


Trp Val Pro Thr Ile Met Glu Glu Asp Ala Phe Gln Leu Pro Gly Leu 
        675                 680                 685             


Gly Thr Pro Pro Ile Thr Lys Leu Thr Val Leu Glu Glu Asp Glu Lys 
    690                 695                 700                 


Lys Pro Val Pro Trp Glu Ser His Asn Ser Ser Glu Thr Cys Gly Leu 
705                 710                 715                 720 


Pro Thr Leu Val Gln Thr Tyr Val Leu Gln Gly Asp Pro Arg Ala Val 
                725                 730                 735     


Ser Thr Gln Pro Gln Ser Gln Ser Gly Thr Ser Asp Gln Val Leu Tyr 
            740                 745                 750         


Gly Gln Leu Leu Gly Ser Pro Thr Ser Pro Gly Pro Gly His Tyr Leu 
        755                 760                 765             


Arg Cys Asp Ser Thr Gln Pro Leu Leu Ala Gly Leu Thr Pro Ser Pro 
    770                 775                 780                 


Lys Ser Tyr Glu Asn Leu Trp Phe Gln Ala Ser Pro Leu Gly Thr Leu 
785                 790                 795                 800 


Val Thr Pro Ala Pro Ser Gln Glu Asp Asp Cys Val Phe Gly Pro Leu 
                805                 810                 815     


Leu Asn Phe Pro Leu Leu Gln Gly Ile Arg Val His Gly Met Glu Ala 
            820                 825                 830         


Leu Gly Ser Phe 
        835     


<210>  17
<211>  319
<212>  PRT
<213>  artificial

<220>
<223>  Ig and CRH domains of Macaca fascicularis G-CSFR (cynoG-CSFR) 
       with a C-terminal polyhistidine tag

<400>  17

Glu Cys Gly His Ile Ser Val Ser Ala Pro Ile Val His Leu Gly Asp 
1               5                   10                  15      


Pro Ile Thr Ala Ser Cys Ile Ile Lys Gln Asn Cys Ser His Leu Asp 
            20                  25                  30          


Leu Glu Pro Gln Ile Leu Trp Arg Leu Gly Ala Glu Leu Gln Pro Gly 
        35                  40                  45              


Gly Arg Gln Gln Arg Leu Ser Asp Gly Ser Gln Gln Ser Thr Ile Thr 
    50                  55                  60                  


Leu Pro His Leu Asn His Thr Arg Ala Phe Leu Ser Cys Ala Leu Asn 
65                  70                  75                  80  


Trp Gly Asn Ser Leu Gln Ile Leu Asp Gln Val Glu Leu Arg Ala Gly 
                85                  90                  95      


Tyr Pro Pro Ala Val Pro Arg Asn Leu Ser Cys Leu Met Asn Leu Thr 
            100                 105                 110         


Thr Ser Ser Leu Ile Cys Gln Trp Glu Pro Gly Pro Glu Thr His Leu 
        115                 120                 125             


Pro Thr Ser Phe Thr Leu Lys Ser Phe Lys Ser Arg Gly Asn Cys Gln 
    130                 135                 140                 


Thr Gln Gly Asp Ser Ile Met Asp Cys Val Pro Glu Asp Gly Gln Ser 
145                 150                 155                 160 


His Cys Ser Ile Pro Arg Arg His Leu Leu Leu Tyr Gln Asn Met Gly 
                165                 170                 175     


Ile Trp Val Gln Ala Glu Asn Ala Leu Gly Thr Ser Met Ser Pro Gln 
            180                 185                 190         


Leu Cys Leu Glu Pro Met Asp Val Val Lys Leu Glu Pro Pro Met Leu 
        195                 200                 205             


Arg Thr Met Asp Pro Ser Pro Glu Ala Ala Pro Pro Gln Ala Gly Cys 
    210                 215                 220                 


Leu Gln Leu Ser Trp Glu Pro Trp Gln Pro Ala Leu His Ile Asn Gln 
225                 230                 235                 240 


Lys Cys Glu Leu Arg His Lys Pro Gln Ser Gly Glu Ala Ser Trp Ala 
                245                 250                 255     


Leu Val Gly Pro Leu Pro Leu Glu Ala Leu Arg Tyr Glu Leu Cys Gly 
            260                 265                 270         


Leu Leu Pro Ala Thr Ala Tyr Thr Leu Gln Ile Arg Cys Ile Arg Trp 
        275                 280                 285             


Pro Leu Pro Gly His Trp Ser Asn Trp Ser Pro Ser Leu Glu Leu Arg 
    290                 295                 300                 


Thr Thr Glu Arg Ala Pro Thr His His His His His His His His 
305                 310                 315                 


<210>  18
<211>  444
<212>  PRT
<213>  artificial

<220>
<223>  C1.2G heavy chain IgG4 with S241P mutation and lacking C-terminal
       lysine residue

<400>  18

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Leu Tyr 
            20                  25                  30          


Trp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ser Ile Ser Ser Ser Gly Gly Val Thr Pro Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Leu Gly Glu Leu Gly Trp Phe Asp Pro Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
        115                 120                 125             


Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly 
    130                 135                 140                 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
145                 150                 155                 160 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
                165                 170                 175     


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
            180                 185                 190         


Ser Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser 
        195                 200                 205             


Asn Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys 
    210                 215                 220                 


Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu 
225                 230                 235                 240 


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
                245                 250                 255     


Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln 
            260                 265                 270         


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
        275                 280                 285             


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
    290                 295                 300                 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
305                 310                 315                 320 


Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys 
                325                 330                 335     


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
            340                 345                 350         


Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
        355                 360                 365             


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
    370                 375                 380                 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
385                 390                 395                 400 


Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln 
                405                 410                 415     


Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
            420                 425                 430         


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly 
        435                 440                 


