                         SEQUENCE LISTING

<110>  Editas Medicine
 
<120>  SYSTEMS AND METHODS FOR THE TREATMENT OF HEMOGLOBINOPATHIES

<130>  118945.8013.WO02

<150>  US 62/643,168
<151>  2018-03-14

<150>  US 62/767,488
<151>  2018-11-14

<150>  US 62/773,073
<151>  2018-11-29

<160>  1265  

<170>  PatentIn version 3.5

<210>  1
<211>  1345
<212>  PRT
<213>  Streptococcus mutans


<220>
<221>  misc_feature
<222>  (10)..(21)
<223>  N-terminal RuvC-like domain

<220>
<221>  misc_feature
<222>  (759)..(766)
<223>  RuvC-like domain

<220>
<221>  misc_feature
<222>  (837)..(863)
<223>  HNH-like domain

<220>
<221>  misc_feature
<222>  (982)..(989)
<223>  RuvC-like domain

<400>  1

Met Lys Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Val Thr Asp Asp Tyr Lys Val Pro Ala Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Lys Ser His Ile Glu Lys Asn Leu Leu 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Asn Thr Ala Glu Asp Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Glu Glu Met Gly Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Asp Ser Phe Leu Val Thr Glu Asp Lys Arg 
            100                 105                 110         


Gly Glu Arg His Pro Ile Phe Gly Asn Leu Glu Glu Glu Val Lys Tyr 
        115                 120                 125             


His Glu Asn Phe Pro Thr Ile Tyr His Leu Arg Gln Tyr Leu Ala Asp 
    130                 135                 140                 


Asn Pro Glu Lys Val Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Lys Phe Asp Thr 
                165                 170                 175     


Arg Asn Asn Asp Val Gln Arg Leu Phe Gln Glu Phe Leu Ala Val Tyr 
            180                 185                 190         


Asp Asn Thr Phe Glu Asn Ser Ser Leu Gln Glu Gln Asn Val Gln Val 
        195                 200                 205             


Glu Glu Ile Leu Thr Asp Lys Ile Ser Lys Ser Ala Lys Lys Asp Arg 
    210                 215                 220                 


Val Leu Lys Leu Phe Pro Asn Glu Lys Ser Asn Gly Arg Phe Ala Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Lys Lys His Phe 
                245                 250                 255     


Glu Leu Glu Glu Lys Ala Pro Leu Gln Phe Ser Lys Asp Thr Tyr Glu 
            260                 265                 270         


Glu Glu Leu Glu Val Leu Leu Ala Gln Ile Gly Asp Asn Tyr Ala Glu 
        275                 280                 285             


Leu Phe Leu Ser Ala Lys Lys Leu Tyr Asp Ser Ile Leu Leu Ser Gly 
    290                 295                 300                 


Ile Leu Thr Val Thr Asp Val Gly Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Gln Arg Tyr Asn Glu His Gln Met Asp Leu Ala Gln Leu Lys 
                325                 330                 335     


Gln Phe Ile Arg Gln Lys Leu Ser Asp Lys Tyr Asn Glu Val Phe Ser 
            340                 345                 350         


Asp Val Ser Lys Asp Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Ala Phe Tyr Lys Tyr Leu Lys Gly Leu Leu Asn Lys Ile Glu 
    370                 375                 380                 


Gly Ser Gly Tyr Phe Leu Asp Lys Ile Glu Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Ile Arg Arg Gln Ala Glu Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Asp Asn Gln Asp Arg Ile Glu Lys Leu Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Lys Ser Asp Phe Ala Trp 
    450                 455                 460                 


Leu Ser Arg Lys Ser Ala Asp Lys Ile Thr Pro Trp Asn Phe Asp Glu 
465                 470                 475                 480 


Ile Val Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Asn Tyr Asp Leu Tyr Leu Pro Asn Gln Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Lys Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Lys Thr Glu Gln Gly Lys Thr Ala Phe Phe Asp Ala Asn Met Lys 
    530                 535                 540                 


Gln Glu Ile Phe Asp Gly Val Phe Lys Val Tyr Arg Lys Val Thr Lys 
545                 550                 555                 560 


Asp Lys Leu Met Asp Phe Leu Glu Lys Glu Phe Asp Glu Phe Arg Ile 
                565                 570                 575     


Val Asp Leu Thr Gly Leu Asp Lys Glu Asn Lys Val Phe Asn Ala Ser 
            580                 585                 590         


Tyr Gly Thr Tyr His Asp Leu Cys Lys Ile Leu Asp Lys Asp Phe Leu 
        595                 600                 605             


Asp Asn Ser Lys Asn Glu Lys Ile Leu Glu Asp Ile Val Leu Thr Leu 
    610                 615                 620                 


Thr Leu Phe Glu Asp Arg Glu Met Ile Arg Lys Arg Leu Glu Asn Tyr 
625                 630                 635                 640 


Ser Asp Leu Leu Thr Lys Glu Gln Val Lys Lys Leu Glu Arg Arg His 
                645                 650                 655     


Tyr Thr Gly Trp Gly Arg Leu Ser Ala Glu Leu Ile His Gly Ile Arg 
            660                 665                 670         


Asn Lys Glu Ser Arg Lys Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly 
        675                 680                 685             


Asn Ser Asn Arg Asn Phe Met Gln Leu Ile Asn Asp Asp Ala Leu Ser 
    690                 695                 700                 


Phe Lys Glu Glu Ile Ala Lys Ala Gln Val Ile Gly Glu Thr Asp Asn 
705                 710                 715                 720 


Leu Asn Gln Val Val Ser Asp Ile Ala Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val Lys Ile Met 
            740                 745                 750         


Gly His Gln Pro Glu Asn Ile Val Val Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Phe Thr Asn Gln Gly Arg Arg Asn Ser Gln Gln Arg Leu Lys Gly Leu 
    770                 775                 780                 


Thr Asp Ser Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Ser Gln Leu Gln Asn Asp Arg Leu Phe Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Thr Gly Glu Glu Leu Asp Ile Asp Tyr 
            820                 825                 830         


Leu Ser Gln Tyr Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys 
        835                 840                 845             


Asp Asn Ser Ile Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asp Val Pro Ser Lys Asp Val Val Arg Lys Met Lys 
865                 870                 875                 880 


Ser Tyr Trp Ser Lys Leu Leu Ser Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr Asp Asp Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Arg Ile Leu Asp Glu Arg Phe Asn Thr Glu Thr Asp 
    930                 935                 940                 


Glu Asn Asn Lys Lys Ile Arg Gln Val Lys Ile Val Thr Leu Lys Ser 
945                 950                 955                 960 


Asn Leu Val Ser Asn Phe Arg Lys Glu Phe Glu Leu Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asp Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Ile Gly Lys Ala Leu Leu Gly Val  Tyr Pro Gln Leu Glu  Pro Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Pro His  Phe His Gly His Lys  Glu Asn Lys 
    1010                 1015                 1020             


Ala Thr  Ala Lys Lys Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe 
    1025                 1030                 1035             


Lys Lys  Asp Asp Val Arg Thr  Asp Lys Asn Gly Glu  Ile Ile Trp 
    1040                 1045                 1050             


Lys Lys  Asp Glu His Ile Ser  Asn Ile Lys Lys Val  Leu Ser Tyr 
    1055                 1060                 1065             


Pro Gln  Val Asn Ile Val Lys  Lys Val Glu Glu Gln  Thr Gly Gly 
    1070                 1075                 1080             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Gly Asn Ser  Asp Lys Leu 
    1085                 1090                 1095             


Ile Pro  Arg Lys Thr Lys Lys  Phe Tyr Trp Asp Thr  Lys Lys Tyr 
    1100                 1105                 1110             


Gly Gly  Phe Asp Ser Pro Ile  Val Ala Tyr Ser Ile  Leu Val Ile 
    1115                 1120                 1125             


Ala Asp  Ile Glu Lys Gly Lys  Ser Lys Lys Leu Lys  Thr Val Lys 
    1130                 1135                 1140             


Ala Leu  Val Gly Val Thr Ile  Met Glu Lys Met Thr  Phe Glu Arg 
    1145                 1150                 1155             


Asp Pro  Val Ala Phe Leu Glu  Arg Lys Gly Tyr Arg  Asn Val Gln 
    1160                 1165                 1170             


Glu Glu  Asn Ile Ile Lys Leu  Pro Lys Tyr Ser Leu  Phe Lys Leu 
    1175                 1180                 1185             


Glu Asn  Gly Arg Lys Arg Leu  Leu Ala Ser Ala Arg  Glu Leu Gln 
    1190                 1195                 1200             


Lys Gly  Asn Glu Ile Val Leu  Pro Asn His Leu Gly  Thr Leu Leu 
    1205                 1210                 1215             


Tyr His  Ala Lys Asn Ile His  Lys Val Asp Glu Pro  Lys His Leu 
    1220                 1225                 1230             


Asp Tyr  Val Asp Lys His Lys  Asp Glu Phe Lys Glu  Leu Leu Asp 
    1235                 1240                 1245             


Val Val  Ser Asn Phe Ser Lys  Lys Tyr Thr Leu Ala  Glu Gly Asn 
    1250                 1255                 1260             


Leu Glu  Lys Ile Lys Glu Leu  Tyr Ala Gln Asn Asn  Gly Glu Asp 
    1265                 1270                 1275             


Leu Lys  Glu Leu Ala Ser Ser  Phe Ile Asn Leu Leu  Thr Phe Thr 
    1280                 1285                 1290             


Ala Ile  Gly Ala Pro Ala Thr  Phe Lys Phe Phe Asp  Lys Asn Ile 
    1295                 1300                 1305             


Asp Arg  Lys Arg Tyr Thr Ser  Thr Thr Glu Ile Leu  Asn Ala Thr 
    1310                 1315                 1320             


Leu Ile  His Gln Ser Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp 
    1325                 1330                 1335             


Leu Asn  Lys Leu Gly Gly Asp  
    1340                 1345 


<210>  2
<211>  1368
<212>  PRT
<213>  Streptococcus pyogenes


<220>
<221>  misc_feature
<222>  (10)..(21)
<223>  N-terminal RuvC-like domain

<220>
<221>  misc_feature
<222>  (759)..(766)
<223>  RuvC-like domain

<220>
<221>  misc_feature
<222>  (837)..(863)
<223>  HNH-like domain

<220>
<221>  misc_feature
<222>  (982)..(989)
<223>  RuvC-like domain

<400>  2

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  3
<211>  4107
<212>  DNA
<213>  Streptococcus pyogenes

<400>  3
atggataaaa agtacagcat cgggctggac atcggtacaa actcagtggg gtgggccgtg       60

attacggacg agtacaaggt accctccaaa aaatttaaag tgctgggtaa cacggacaga      120

cactctataa agaaaaatct tattggagcc ttgctgttcg actcaggcga gacagccgaa      180

gccacaaggt tgaagcggac cgccaggagg cggtatacca ggagaaagaa ccgcatatgc      240

tacctgcaag aaatcttcag taacgagatg gcaaaggttg acgatagctt tttccatcgc      300

ctggaagaat cctttcttgt tgaggaagac aagaagcacg aacggcaccc catctttggc      360

aatattgtcg acgaagtggc atatcacgaa aagtacccga ctatctacca cctcaggaag      420

aagctggtgg actctaccga taaggcggac ctcagactta tttatttggc actcgcccac      480

atgattaaat ttagaggaca tttcttgatc gagggcgacc tgaacccgga caacagtgac      540

gtcgataagc tgttcatcca acttgtgcag acctacaatc aactgttcga agaaaaccct      600

ataaatgctt caggagtcga cgctaaagca atcctgtccg cgcgcctctc aaaatctaga      660

agacttgaga atctgattgc tcagttgccc ggggaaaaga aaaatggatt gtttggcaac      720

ctgatcgccc tcagtctcgg actgacccca aatttcaaaa gtaacttcga cctggccgaa      780

gacgctaagc tccagctgtc caaggacaca tacgatgacg acctcgacaa tctgctggcc      840

cagattgggg atcagtacgc cgatctcttt ttggcagcaa agaacctgtc cgacgccatc      900

ctgttgagcg atatcttgag agtgaacacc gaaattacta aagcacccct tagcgcatct      960

atgatcaagc ggtacgacga gcatcatcag gatctgaccc tgctgaaggc tcttgtgagg     1020

caacagctcc ccgaaaaata caaggaaatc ttctttgacc agagcaaaaa cggctacgct     1080

ggctatatag atggtggggc cagtcaggag gaattctata aattcatcaa gcccattctc     1140

gagaaaatgg acggcacaga ggagttgctg gtcaaactta acagggagga cctgctgcgg     1200

aagcagcgga cctttgacaa cgggtctatc ccccaccaga ttcatctggg cgaactgcac     1260

gcaatcctga ggaggcagga ggatttttat ccttttctta aagataaccg cgagaaaata     1320

gaaaagattc ttacattcag gatcccgtac tacgtgggac ctctcgcccg gggcaattca     1380

cggtttgcct ggatgacaag gaagtcagag gagactatta caccttggaa cttcgaagaa     1440

gtggtggaca agggtgcatc tgcccagtct ttcatcgagc ggatgacaaa ttttgacaag     1500

aacctcccta atgagaaggt gctgcccaaa cattctctgc tctacgagta ctttaccgtc     1560

tacaatgaac tgactaaagt caagtacgtc accgagggaa tgaggaagcc ggcattcctt     1620

agtggagaac agaagaaggc gattgtagac ctgttgttca agaccaacag gaaggtgact     1680

gtgaagcaac ttaaagaaga ctactttaag aagatcgaat gttttgacag tgtggaaatt     1740

tcaggggttg aagaccgctt caatgcgtca ttggggactt accatgatct tctcaagatc     1800

ataaaggaca aagacttcct ggacaacgaa gaaaatgagg atattctcga agacatcgtc     1860

ctcaccctga ccctgttcga agacagggaa atgatagaag agcgcttgaa aacctatgcc     1920

cacctcttcg acgataaagt tatgaagcag ctgaagcgca ggagatacac aggatgggga     1980

agattgtcaa ggaagctgat caatggaatt agggataaac agagtggcaa gaccatactg     2040

gatttcctca aatctgatgg cttcgccaat aggaacttca tgcaactgat tcacgatgac     2100

tctcttacct tcaaggagga cattcaaaag gctcaggtga gcgggcaggg agactccctt     2160

catgaacaca tcgcgaattt ggcaggttcc cccgctatta aaaagggcat ccttcaaact     2220

gtcaaggtgg tggatgaatt ggtcaaggta atgggcagac ataagccaga aaatattgtg     2280

atcgagatgg cccgcgaaaa ccagaccaca cagaagggcc agaaaaatag tagagagcgg     2340

atgaagagga tcgaggaggg catcaaagag ctgggatctc agattctcaa agaacacccc     2400

gtagaaaaca cacagctgca gaacgaaaaa ttgtacttgt actatctgca gaacggcaga     2460

gacatgtacg tcgaccaaga acttgatatt aatagactgt ccgactatga cgtagaccat     2520

atcgtgcccc agtccttcct gaaggacgac tccattgata acaaagtctt gacaagaagc     2580

gacaagaaca ggggtaaaag tgataatgtg cctagcgagg aggtggtgaa aaaaatgaag     2640

aactactggc gacagctgct taatgcaaag ctcattacac aacggaagtt cgataatctg     2700

acgaaagcag agagaggtgg cttgtctgag ttggacaagg cagggtttat taagcggcag     2760

ctggtggaaa ctaggcagat cacaaagcac gtggcgcaga ttttggacag ccggatgaac     2820

acaaaatacg acgaaaatga taaactgata cgagaggtca aagttatcac gctgaaaagc     2880

aagctggtgt ccgattttcg gaaagacttc cagttctaca aagttcgcga gattaataac     2940

taccatcatg ctcacgatgc gtacctgaac gctgttgtcg ggaccgcctt gataaagaag     3000

tacccaaagc tggaatccga gttcgtatac ggggattaca aagtgtacga tgtgaggaaa     3060

atgatagcca agtccgagca ggagattgga aaggccacag ctaagtactt cttttattct     3120

aacatcatga atttttttaa gacggaaatt accctggcca acggagagat cagaaagcgg     3180

ccccttatag agacaaatgg tgaaacaggt gaaatcgtct gggataaggg cagggatttc     3240

gctactgtga ggaaggtgct gagtatgcca caggtaaata tcgtgaaaaa aaccgaagta     3300

cagaccggag gattttccaa ggaaagcatt ttgcctaaaa gaaactcaga caagctcatc     3360

gcccgcaaga aagattggga ccctaagaaa tacgggggat ttgactcacc caccgtagcc     3420

tattctgtgc tggtggtagc taaggtggaa aaaggaaagt ctaagaagct gaagtccgtg     3480

aaggaactct tgggaatcac tatcatggaa agatcatcct ttgaaaagaa ccctatcgat     3540

ttcctggagg ctaagggtta caaggaggtc aagaaagacc tcatcattaa actgccaaaa     3600

tactctctct tcgagctgga aaatggcagg aagagaatgt tggccagcgc cggagagctg     3660

caaaagggaa acgagcttgc tctgccctcc aaatatgtta attttctcta tctcgcttcc     3720

cactatgaaa agctgaaagg gtctcccgaa gataacgagc agaagcagct gttcgtcgaa     3780

cagcacaagc actatctgga tgaaataatc gaacaaataa gcgagttcag caaaagggtt     3840

atcctggcgg atgctaattt ggacaaagta ctgtctgctt ataacaagca ccgggataag     3900

cctattaggg aacaagccga gaatataatt cacctcttta cactcacgaa tctcggagcc     3960

cccgccgcct tcaaatactt tgatacgact atcgaccgga aacggtatac cagtaccaaa     4020

gaggtcctcg atgccaccct catccaccag tcaattactg gcctgtacga aacacggatc     4080

gacctctctc aactgggcgg cgactag                                         4107


<210>  4
<211>  1388
<212>  PRT
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (10)..(21)
<223>  N-terminal RuvC-like domain

<220>
<221>  misc_feature
<222>  (760)..(767)
<223>  RuvC-like domain

<220>
<221>  misc_feature
<222>  (844)..(870)
<223>  HNH-like domain

<220>
<221>  misc_feature
<222>  (989)..(996)
<223>  RuvC-like domain

<400>  4

Met Thr Lys Pro Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Thr Thr Asp Asn Tyr Lys Val Pro Ser Lys Lys Met 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Ser Lys Lys Tyr Ile Lys Lys Asn Leu Leu 
        35                  40                  45              


Gly Val Leu Leu Phe Asp Ser Gly Ile Thr Ala Glu Gly Arg Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Arg Asn Arg Ile Leu 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Thr Glu Met Ala Thr Leu Asp Asp Ala 
                85                  90                  95      


Phe Phe Gln Arg Leu Asp Asp Ser Phe Leu Val Pro Asp Asp Lys Arg 
            100                 105                 110         


Asp Ser Lys Tyr Pro Ile Phe Gly Asn Leu Val Glu Glu Lys Ala Tyr 
        115                 120                 125             


His Asp Glu Phe Pro Thr Ile Tyr His Leu Arg Lys Tyr Leu Ala Asp 
    130                 135                 140                 


Ser Thr Lys Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Tyr Arg Gly His Phe Leu Ile Glu Gly Glu Phe Asn Ser 
                165                 170                 175     


Lys Asn Asn Asp Ile Gln Lys Asn Phe Gln Asp Phe Leu Asp Thr Tyr 
            180                 185                 190         


Asn Ala Ile Phe Glu Ser Asp Leu Ser Leu Glu Asn Ser Lys Gln Leu 
        195                 200                 205             


Glu Glu Ile Val Lys Asp Lys Ile Ser Lys Leu Glu Lys Lys Asp Arg 
    210                 215                 220                 


Ile Leu Lys Leu Phe Pro Gly Glu Lys Asn Ser Gly Ile Phe Ser Glu 
225                 230                 235                 240 


Phe Leu Lys Leu Ile Val Gly Asn Gln Ala Asp Phe Arg Lys Cys Phe 
                245                 250                 255     


Asn Leu Asp Glu Lys Ala Ser Leu His Phe Ser Lys Glu Ser Tyr Asp 
            260                 265                 270         


Glu Asp Leu Glu Thr Leu Leu Gly Tyr Ile Gly Asp Asp Tyr Ser Asp 
        275                 280                 285             


Val Phe Leu Lys Ala Lys Lys Leu Tyr Asp Ala Ile Leu Leu Ser Gly 
    290                 295                 300                 


Phe Leu Thr Val Thr Asp Asn Glu Thr Glu Ala Pro Leu Ser Ser Ala 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asn Glu His Lys Glu Asp Leu Ala Leu Leu Lys 
                325                 330                 335     


Glu Tyr Ile Arg Asn Ile Ser Leu Lys Thr Tyr Asn Glu Val Phe Lys 
            340                 345                 350         


Asp Asp Thr Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Lys Thr Asn 
        355                 360                 365             


Gln Glu Asp Phe Tyr Val Tyr Leu Lys Lys Leu Leu Ala Glu Phe Glu 
    370                 375                 380                 


Gly Ala Asp Tyr Phe Leu Glu Lys Ile Asp Arg Glu Asp Phe Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro Tyr Gln Ile His Leu 
                405                 410                 415     


Gln Glu Met Arg Ala Ile Leu Asp Lys Gln Ala Lys Phe Tyr Pro Phe 
            420                 425                 430         


Leu Ala Lys Asn Lys Glu Arg Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Asp Phe Ala Trp 
    450                 455                 460                 


Ser Ile Arg Lys Arg Asn Glu Lys Ile Thr Pro Trp Asn Phe Glu Asp 
465                 470                 475                 480 


Val Ile Asp Lys Glu Ser Ser Ala Glu Ala Phe Ile Asn Arg Met Thr 
                485                 490                 495     


Ser Phe Asp Leu Tyr Leu Pro Glu Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Thr Phe Asn Val Tyr Asn Glu Leu Thr Lys Val Arg 
        515                 520                 525             


Phe Ile Ala Glu Ser Met Arg Asp Tyr Gln Phe Leu Asp Ser Lys Gln 
    530                 535                 540                 


Lys Lys Asp Ile Val Arg Leu Tyr Phe Lys Asp Lys Arg Lys Val Thr 
545                 550                 555                 560 


Asp Lys Asp Ile Ile Glu Tyr Leu His Ala Ile Tyr Gly Tyr Asp Gly 
                565                 570                 575     


Ile Glu Leu Lys Gly Ile Glu Lys Gln Phe Asn Ser Ser Leu Ser Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Asn Ile Ile Asn Asp Lys Glu Phe Leu Asp Asp 
        595                 600                 605             


Ser Ser Asn Glu Ala Ile Ile Glu Glu Ile Ile His Thr Leu Thr Ile 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Lys Gln Arg Leu Ser Lys Phe Glu Asn 
625                 630                 635                 640 


Ile Phe Asp Lys Ser Val Leu Lys Lys Leu Ser Arg Arg His Tyr Thr 
                645                 650                 655     


Gly Trp Gly Lys Leu Ser Ala Lys Leu Ile Asn Gly Ile Arg Asp Glu 
            660                 665                 670         


Lys Ser Gly Asn Thr Ile Leu Asp Tyr Leu Ile Asp Asp Gly Ile Ser 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ala Leu Ser Phe Lys 
    690                 695                 700                 


Lys Lys Ile Gln Lys Ala Gln Ile Ile Gly Asp Glu Asp Lys Gly Asn 
705                 710                 715                 720 


Ile Lys Glu Val Val Lys Ser Leu Pro Gly Ser Pro Ala Ile Lys Lys 
                725                 730                 735     


Gly Ile Leu Gln Ser Ile Lys Ile Val Asp Glu Leu Val Lys Val Met 
            740                 745                 750         


Gly Gly Arg Lys Pro Glu Ser Ile Val Val Glu Met Ala Arg Glu Asn 
        755                 760                 765             


Gln Tyr Thr Asn Gln Gly Lys Ser Asn Ser Gln Gln Arg Leu Lys Arg 
    770                 775                 780                 


Leu Glu Lys Ser Leu Lys Glu Leu Gly Ser Lys Ile Leu Lys Glu Asn 
785                 790                 795                 800 


Ile Pro Ala Lys Leu Ser Lys Ile Asp Asn Asn Ala Leu Gln Asn Asp 
                805                 810                 815     


Arg Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly 
            820                 825                 830         


Asp Asp Leu Asp Ile Asp Arg Leu Ser Asn Tyr Asp Ile Asp His Ile 
        835                 840                 845             


Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile Asp Asn Lys Val Leu 
    850                 855                 860                 


Val Ser Ser Ala Ser Asn Arg Gly Lys Ser Asp Asp Val Pro Ser Leu 
865                 870                 875                 880 


Glu Val Val Lys Lys Arg Lys Thr Phe Trp Tyr Gln Leu Leu Lys Ser 
                885                 890                 895     


Lys Leu Ile Ser Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg 
            900                 905                 910         


Gly Gly Leu Ser Pro Glu Asp Lys Ala Gly Phe Ile Gln Arg Gln Leu 
        915                 920                 925             


Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Arg Leu Leu Asp Glu 
    930                 935                 940                 


Lys Phe Asn Asn Lys Lys Asp Glu Asn Asn Arg Ala Val Arg Thr Val 
945                 950                 955                 960 


Lys Ile Ile Thr Leu Lys Ser Thr Leu Val Ser Gln Phe Arg Lys Asp 
                965                 970                 975     


Phe Glu Leu Tyr Lys Val Arg Glu Ile Asn Asp Phe His His Ala His 
            980                 985                 990         


Asp Ala Tyr Leu Asn Ala Val Val  Ala Ser Ala Leu Leu  Lys Lys Tyr 
        995                 1000                 1005             


Pro Lys  Leu Glu Pro Glu Phe  Val Tyr Gly Asp Tyr  Pro Lys Tyr 
    1010                 1015                 1020             


Asn Ser  Phe Arg Glu Arg Lys  Ser Ala Thr Glu Lys  Val Tyr Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Ile  Phe Lys Lys Ser Ile  Ser Leu Ala 
    1040                 1045                 1050             


Asp Gly  Arg Val Ile Glu Arg  Pro Leu Ile Glu Val  Asn Glu Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ser Val Trp Asn  Lys Glu Ser Asp Leu  Ala Thr Val 
    1070                 1075                 1080             


Arg Arg  Val Leu Ser Tyr Pro  Gln Val Asn Val Val  Lys Lys Val 
    1085                 1090                 1095             


Glu Glu  Gln Asn His Gly Leu  Asp Arg Gly Lys Pro  Lys Gly Leu 
    1100                 1105                 1110             


Phe Asn  Ala Asn Leu Ser Ser  Lys Pro Lys Pro Asn  Ser Asn Glu 
    1115                 1120                 1125             


Asn Leu  Val Gly Ala Lys Glu  Tyr Leu Asp Pro Lys  Lys Tyr Gly 
    1130                 1135                 1140             


Gly Tyr  Ala Gly Ile Ser Asn  Ser Phe Thr Val Leu  Val Lys Gly 
    1145                 1150                 1155             


Thr Ile  Glu Lys Gly Ala Lys  Lys Lys Ile Thr Asn  Val Leu Glu 
    1160                 1165                 1170             


Phe Gln  Gly Ile Ser Ile Leu  Asp Arg Ile Asn Tyr  Arg Lys Asp 
    1175                 1180                 1185             


Lys Leu  Asn Phe Leu Leu Glu  Lys Gly Tyr Lys Asp  Ile Glu Leu 
    1190                 1195                 1200             


Ile Ile  Glu Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Ser Asp Gly 
    1205                 1210                 1215             


Ser Arg  Arg Met Leu Ala Ser  Ile Leu Ser Thr Asn  Asn Lys Arg 
    1220                 1225                 1230             


Gly Glu  Ile His Lys Gly Asn  Gln Ile Phe Leu Ser  Gln Lys Phe 
    1235                 1240                 1245             


Val Lys  Leu Leu Tyr His Ala  Lys Arg Ile Ser Asn  Thr Ile Asn 
    1250                 1255                 1260             


Glu Asn  His Arg Lys Tyr Val  Glu Asn His Lys Lys  Glu Phe Glu 
    1265                 1270                 1275             


Glu Leu  Phe Tyr Tyr Ile Leu  Glu Phe Asn Glu Asn  Tyr Val Gly 
    1280                 1285                 1290             


Ala Lys  Lys Asn Gly Lys Leu  Leu Asn Ser Ala Phe  Gln Ser Trp 
    1295                 1300                 1305             


Gln Asn  His Ser Ile Asp Glu  Leu Cys Ser Ser Phe  Ile Gly Pro 
    1310                 1315                 1320             


Thr Gly  Ser Glu Arg Lys Gly  Leu Phe Glu Leu Thr  Ser Arg Gly 
    1325                 1330                 1335             


Ser Ala  Ala Asp Phe Glu Phe  Leu Gly Val Lys Ile  Pro Arg Tyr 
    1340                 1345                 1350             


Arg Asp  Tyr Thr Pro Ser Ser  Leu Leu Lys Asp Ala  Thr Leu Ile 
    1355                 1360                 1365             


His Gln  Ser Val Thr Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ala 
    1370                 1375                 1380             


Lys Leu  Gly Glu Gly 
    1385             


<210>  5
<211>  1334
<212>  PRT
<213>  Listeria innocua


<220>
<221>  misc_feature
<222>  (10)..(21)
<223>  N-terminal RuvC-like domain

<220>
<221>  misc_feature
<222>  (762)..(769)
<223>  RuvC-like domain

<220>
<221>  misc_feature
<222>  (840)..(866)
<223>  HNH-like domain

<220>
<221>  misc_feature
<222>  (985)..(992)
<223>  RuvC-like domain

<400>  5

Met Lys Lys Pro Tyr Thr Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Leu Thr Asp Gln Tyr Asp Leu Val Lys Arg Lys Met 
            20                  25                  30          


Lys Ile Ala Gly Asp Ser Glu Lys Lys Gln Ile Lys Lys Asn Phe Trp 
        35                  40                  45              


Gly Val Arg Leu Phe Asp Glu Gly Gln Thr Ala Ala Asp Arg Arg Met 
    50                  55                  60                  


Ala Arg Thr Ala Arg Arg Arg Ile Glu Arg Arg Arg Asn Arg Ile Ser 
65                  70                  75                  80  


Tyr Leu Gln Gly Ile Phe Ala Glu Glu Met Ser Lys Thr Asp Ala Asn 
                85                  90                  95      


Phe Phe Cys Arg Leu Ser Asp Ser Phe Tyr Val Asp Asn Glu Lys Arg 
            100                 105                 110         


Asn Ser Arg His Pro Phe Phe Ala Thr Ile Glu Glu Glu Val Glu Tyr 
        115                 120                 125             


His Lys Asn Tyr Pro Thr Ile Tyr His Leu Arg Glu Glu Leu Val Asn 
    130                 135                 140                 


Ser Ser Glu Lys Ala Asp Leu Arg Leu Val Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Ile Ile Lys Tyr Arg Gly Asn Phe Leu Ile Glu Gly Ala Leu Asp Thr 
                165                 170                 175     


Gln Asn Thr Ser Val Asp Gly Ile Tyr Lys Gln Phe Ile Gln Thr Tyr 
            180                 185                 190         


Asn Gln Val Phe Ala Ser Gly Ile Glu Asp Gly Ser Leu Lys Lys Leu 
        195                 200                 205             


Glu Asp Asn Lys Asp Val Ala Lys Ile Leu Val Glu Lys Val Thr Arg 
    210                 215                 220                 


Lys Glu Lys Leu Glu Arg Ile Leu Lys Leu Tyr Pro Gly Glu Lys Ser 
225                 230                 235                 240 


Ala Gly Met Phe Ala Gln Phe Ile Ser Leu Ile Val Gly Ser Lys Gly 
                245                 250                 255     


Asn Phe Gln Lys Pro Phe Asp Leu Ile Glu Lys Ser Asp Ile Glu Cys 
            260                 265                 270         


Ala Lys Asp Ser Tyr Glu Glu Asp Leu Glu Ser Leu Leu Ala Leu Ile 
        275                 280                 285             


Gly Asp Glu Tyr Ala Glu Leu Phe Val Ala Ala Lys Asn Ala Tyr Ser 
    290                 295                 300                 


Ala Val Val Leu Ser Ser Ile Ile Thr Val Ala Glu Thr Glu Thr Asn 
305                 310                 315                 320 


Ala Lys Leu Ser Ala Ser Met Ile Glu Arg Phe Asp Thr His Glu Glu 
                325                 330                 335     


Asp Leu Gly Glu Leu Lys Ala Phe Ile Lys Leu His Leu Pro Lys His 
            340                 345                 350         


Tyr Glu Glu Ile Phe Ser Asn Thr Glu Lys His Gly Tyr Ala Gly Tyr 
        355                 360                 365             


Ile Asp Gly Lys Thr Lys Gln Ala Asp Phe Tyr Lys Tyr Met Lys Met 
    370                 375                 380                 


Thr Leu Glu Asn Ile Glu Gly Ala Asp Tyr Phe Ile Ala Lys Ile Glu 
385                 390                 395                 400 


Lys Glu Asn Phe Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ala Ile 
                405                 410                 415     


Pro His Gln Leu His Leu Glu Glu Leu Glu Ala Ile Leu His Gln Gln 
            420                 425                 430         


Ala Lys Tyr Tyr Pro Phe Leu Lys Glu Asn Tyr Asp Lys Ile Lys Ser 
        435                 440                 445             


Leu Val Thr Phe Arg Ile Pro Tyr Phe Val Gly Pro Leu Ala Asn Gly 
    450                 455                 460                 


Gln Ser Glu Phe Ala Trp Leu Thr Arg Lys Ala Asp Gly Glu Ile Arg 
465                 470                 475                 480 


Pro Trp Asn Ile Glu Glu Lys Val Asp Phe Gly Lys Ser Ala Val Asp 
                485                 490                 495     


Phe Ile Glu Lys Met Thr Asn Lys Asp Thr Tyr Leu Pro Lys Glu Asn 
            500                 505                 510         


Val Leu Pro Lys His Ser Leu Cys Tyr Gln Lys Tyr Leu Val Tyr Asn 
        515                 520                 525             


Glu Leu Thr Lys Val Arg Tyr Ile Asn Asp Gln Gly Lys Thr Ser Tyr 
    530                 535                 540                 


Phe Ser Gly Gln Glu Lys Glu Gln Ile Phe Asn Asp Leu Phe Lys Gln 
545                 550                 555                 560 


Lys Arg Lys Val Lys Lys Lys Asp Leu Glu Leu Phe Leu Arg Asn Met 
                565                 570                 575     


Ser His Val Glu Ser Pro Thr Ile Glu Gly Leu Glu Asp Ser Phe Asn 
            580                 585                 590         


Ser Ser Tyr Ser Thr Tyr His Asp Leu Leu Lys Val Gly Ile Lys Gln 
        595                 600                 605             


Glu Ile Leu Asp Asn Pro Val Asn Thr Glu Met Leu Glu Asn Ile Val 
    610                 615                 620                 


Lys Ile Leu Thr Val Phe Glu Asp Lys Arg Met Ile Lys Glu Gln Leu 
625                 630                 635                 640 


Gln Gln Phe Ser Asp Val Leu Asp Gly Val Val Leu Lys Lys Leu Glu 
                645                 650                 655     


Arg Arg His Tyr Thr Gly Trp Gly Arg Leu Ser Ala Lys Leu Leu Met 
            660                 665                 670         


Gly Ile Arg Asp Lys Gln Ser His Leu Thr Ile Leu Asp Tyr Leu Met 
        675                 680                 685             


Asn Asp Asp Gly Leu Asn Arg Asn Leu Met Gln Leu Ile Asn Asp Ser 
    690                 695                 700                 


Asn Leu Ser Phe Lys Ser Ile Ile Glu Lys Glu Gln Val Thr Thr Ala 
705                 710                 715                 720 


Asp Lys Asp Ile Gln Ser Ile Val Ala Asp Leu Ala Gly Ser Pro Ala 
                725                 730                 735     


Ile Lys Lys Gly Ile Leu Gln Ser Leu Lys Ile Val Asp Glu Leu Val 
            740                 745                 750         


Ser Val Met Gly Tyr Pro Pro Gln Thr Ile Val Val Glu Met Ala Arg 
        755                 760                 765             


Glu Asn Gln Thr Thr Gly Lys Gly Lys Asn Asn Ser Arg Pro Arg Tyr 
    770                 775                 780                 


Lys Ser Leu Glu Lys Ala Ile Lys Glu Phe Gly Ser Gln Ile Leu Lys 
785                 790                 795                 800 


Glu His Pro Thr Asp Asn Gln Glu Leu Arg Asn Asn Arg Leu Tyr Leu 
                805                 810                 815     


Tyr Tyr Leu Gln Asn Gly Lys Asp Met Tyr Thr Gly Gln Asp Leu Asp 
            820                 825                 830         


Ile His Asn Leu Ser Asn Tyr Asp Ile Asp His Ile Val Pro Gln Ser 
        835                 840                 845             


Phe Ile Thr Asp Asn Ser Ile Asp Asn Leu Val Leu Thr Ser Ser Ala 
    850                 855                 860                 


Gly Asn Arg Glu Lys Gly Asp Asp Val Pro Pro Leu Glu Ile Val Arg 
865                 870                 875                 880 


Lys Arg Lys Val Phe Trp Glu Lys Leu Tyr Gln Gly Asn Leu Met Ser 
                885                 890                 895     


Lys Arg Lys Phe Asp Tyr Leu Thr Lys Ala Glu Arg Gly Gly Leu Thr 
            900                 905                 910         


Glu Ala Asp Lys Ala Arg Phe Ile His Arg Gln Leu Val Glu Thr Arg 
        915                 920                 925             


Gln Ile Thr Lys Asn Val Ala Asn Ile Leu His Gln Arg Phe Asn Tyr 
    930                 935                 940                 


Glu Lys Asp Asp His Gly Asn Thr Met Lys Gln Val Arg Ile Val Thr 
945                 950                 955                 960 


Leu Lys Ser Ala Leu Val Ser Gln Phe Arg Lys Gln Phe Gln Leu Tyr 
                965                 970                 975     


Lys Val Arg Asp Val Asn Asp Tyr His His Ala His Asp Ala Tyr Leu 
            980                 985                 990         


Asn Gly Val Val Ala Asn Thr Leu  Leu Lys Val Tyr Pro  Gln Leu Glu 
        995                 1000                 1005             


Pro Glu  Phe Val Tyr Gly Asp  Tyr His Gln Phe Asp  Trp Phe Lys 
    1010                 1015                 1020             


Ala Asn  Lys Ala Thr Ala Lys  Lys Gln Phe Tyr Thr  Asn Ile Met 
    1025                 1030                 1035             


Leu Phe  Phe Ala Gln Lys Asp  Arg Ile Ile Asp Glu  Asn Gly Glu 
    1040                 1045                 1050             


Ile Leu  Trp Asp Lys Lys Tyr  Leu Asp Thr Val Lys  Lys Val Met 
    1055                 1060                 1065             


Ser Tyr  Arg Gln Met Asn Ile  Val Lys Lys Thr Glu  Ile Gln Lys 
    1070                 1075                 1080             


Gly Glu  Phe Ser Lys Ala Thr  Ile Lys Pro Lys Gly  Asn Ser Ser 
    1085                 1090                 1095             


Lys Leu  Ile Pro Arg Lys Thr  Asn Trp Asp Pro Met  Lys Tyr Gly 
    1100                 1105                 1110             


Gly Leu  Asp Ser Pro Asn Met  Ala Tyr Ala Val Val  Ile Glu Tyr 
    1115                 1120                 1125             


Ala Lys  Gly Lys Asn Lys Leu  Val Phe Glu Lys Lys  Ile Ile Arg 
    1130                 1135                 1140             


Val Thr  Ile Met Glu Arg Lys  Ala Phe Glu Lys Asp  Glu Lys Ala 
    1145                 1150                 1155             


Phe Leu  Glu Glu Gln Gly Tyr  Arg Gln Pro Lys Val  Leu Ala Lys 
    1160                 1165                 1170             


Leu Pro  Lys Tyr Thr Leu Tyr  Glu Cys Glu Glu Gly  Arg Arg Arg 
    1175                 1180                 1185             


Met Leu  Ala Ser Ala Asn Glu  Ala Gln Lys Gly Asn  Gln Gln Val 
    1190                 1195                 1200             


Leu Pro  Asn His Leu Val Thr  Leu Leu His His Ala  Ala Asn Cys 
    1205                 1210                 1215             


Glu Val  Ser Asp Gly Lys Ser  Leu Asp Tyr Ile Glu  Ser Asn Arg 
    1220                 1225                 1230             


Glu Met  Phe Ala Glu Leu Leu  Ala His Val Ser Glu  Phe Ala Lys 
    1235                 1240                 1245             


Arg Tyr  Thr Leu Ala Glu Ala  Asn Leu Asn Lys Ile  Asn Gln Leu 
    1250                 1255                 1260             


Phe Glu  Gln Asn Lys Glu Gly  Asp Ile Lys Ala Ile  Ala Gln Ser 
    1265                 1270                 1275             


Phe Val  Asp Leu Met Ala Phe  Asn Ala Met Gly Ala  Pro Ala Ser 
    1280                 1285                 1290             


Phe Lys  Phe Phe Glu Thr Thr  Ile Glu Arg Lys Arg  Tyr Asn Asn 
    1295                 1300                 1305             


Leu Lys  Glu Leu Leu Asn Ser  Thr Ile Ile Tyr Gln  Ser Ile Thr 
    1310                 1315                 1320             


Gly Leu  Tyr Glu Ser Arg Lys  Arg Leu Asp Asp 
    1325                 1330                 


<210>  6
<211>  1053
<212>  PRT
<213>  Staphylococcus aureus

<400>  6

Met Lys Arg Asn Tyr Ile Leu Gly Leu Asp Ile Gly Ile Thr Ser Val 
1               5                   10                  15      


Gly Tyr Gly Ile Ile Asp Tyr Glu Thr Arg Asp Val Ile Asp Ala Gly 
            20                  25                  30          


Val Arg Leu Phe Lys Glu Ala Asn Val Glu Asn Asn Glu Gly Arg Arg 
        35                  40                  45              


Ser Lys Arg Gly Ala Arg Arg Leu Lys Arg Arg Arg Arg His Arg Ile 
    50                  55                  60                  


Gln Arg Val Lys Lys Leu Leu Phe Asp Tyr Asn Leu Leu Thr Asp His 
65                  70                  75                  80  


Ser Glu Leu Ser Gly Ile Asn Pro Tyr Glu Ala Arg Val Lys Gly Leu 
                85                  90                  95      


Ser Gln Lys Leu Ser Glu Glu Glu Phe Ser Ala Ala Leu Leu His Leu 
            100                 105                 110         


Ala Lys Arg Arg Gly Val His Asn Val Asn Glu Val Glu Glu Asp Thr 
        115                 120                 125             


Gly Asn Glu Leu Ser Thr Lys Glu Gln Ile Ser Arg Asn Ser Lys Ala 
    130                 135                 140                 


Leu Glu Glu Lys Tyr Val Ala Glu Leu Gln Leu Glu Arg Leu Lys Lys 
145                 150                 155                 160 


Asp Gly Glu Val Arg Gly Ser Ile Asn Arg Phe Lys Thr Ser Asp Tyr 
                165                 170                 175     


Val Lys Glu Ala Lys Gln Leu Leu Lys Val Gln Lys Ala Tyr His Gln 
            180                 185                 190         


Leu Asp Gln Ser Phe Ile Asp Thr Tyr Ile Asp Leu Leu Glu Thr Arg 
        195                 200                 205             


Arg Thr Tyr Tyr Glu Gly Pro Gly Glu Gly Ser Pro Phe Gly Trp Lys 
    210                 215                 220                 


Asp Ile Lys Glu Trp Tyr Glu Met Leu Met Gly His Cys Thr Tyr Phe 
225                 230                 235                 240 


Pro Glu Glu Leu Arg Ser Val Lys Tyr Ala Tyr Asn Ala Asp Leu Tyr 
                245                 250                 255     


Asn Ala Leu Asn Asp Leu Asn Asn Leu Val Ile Thr Arg Asp Glu Asn 
            260                 265                 270         


Glu Lys Leu Glu Tyr Tyr Glu Lys Phe Gln Ile Ile Glu Asn Val Phe 
        275                 280                 285             


Lys Gln Lys Lys Lys Pro Thr Leu Lys Gln Ile Ala Lys Glu Ile Leu 
    290                 295                 300                 


Val Asn Glu Glu Asp Ile Lys Gly Tyr Arg Val Thr Ser Thr Gly Lys 
305                 310                 315                 320 


Pro Glu Phe Thr Asn Leu Lys Val Tyr His Asp Ile Lys Asp Ile Thr 
                325                 330                 335     


Ala Arg Lys Glu Ile Ile Glu Asn Ala Glu Leu Leu Asp Gln Ile Ala 
            340                 345                 350         


Lys Ile Leu Thr Ile Tyr Gln Ser Ser Glu Asp Ile Gln Glu Glu Leu 
        355                 360                 365             


Thr Asn Leu Asn Ser Glu Leu Thr Gln Glu Glu Ile Glu Gln Ile Ser 
    370                 375                 380                 


Asn Leu Lys Gly Tyr Thr Gly Thr His Asn Leu Ser Leu Lys Ala Ile 
385                 390                 395                 400 


Asn Leu Ile Leu Asp Glu Leu Trp His Thr Asn Asp Asn Gln Ile Ala 
                405                 410                 415     


Ile Phe Asn Arg Leu Lys Leu Val Pro Lys Lys Val Asp Leu Ser Gln 
            420                 425                 430         


Gln Lys Glu Ile Pro Thr Thr Leu Val Asp Asp Phe Ile Leu Ser Pro 
        435                 440                 445             


Val Val Lys Arg Ser Phe Ile Gln Ser Ile Lys Val Ile Asn Ala Ile 
    450                 455                 460                 


Ile Lys Lys Tyr Gly Leu Pro Asn Asp Ile Ile Ile Glu Leu Ala Arg 
465                 470                 475                 480 


Glu Lys Asn Ser Lys Asp Ala Gln Lys Met Ile Asn Glu Met Gln Lys 
                485                 490                 495     


Arg Asn Arg Gln Thr Asn Glu Arg Ile Glu Glu Ile Ile Arg Thr Thr 
            500                 505                 510         


Gly Lys Glu Asn Ala Lys Tyr Leu Ile Glu Lys Ile Lys Leu His Asp 
        515                 520                 525             


Met Gln Glu Gly Lys Cys Leu Tyr Ser Leu Glu Ala Ile Pro Leu Glu 
    530                 535                 540                 


Asp Leu Leu Asn Asn Pro Phe Asn Tyr Glu Val Asp His Ile Ile Pro 
545                 550                 555                 560 


Arg Ser Val Ser Phe Asp Asn Ser Phe Asn Asn Lys Val Leu Val Lys 
                565                 570                 575     


Gln Glu Glu Asn Ser Lys Lys Gly Asn Arg Thr Pro Phe Gln Tyr Leu 
            580                 585                 590         


Ser Ser Ser Asp Ser Lys Ile Ser Tyr Glu Thr Phe Lys Lys His Ile 
        595                 600                 605             


Leu Asn Leu Ala Lys Gly Lys Gly Arg Ile Ser Lys Thr Lys Lys Glu 
    610                 615                 620                 


Tyr Leu Leu Glu Glu Arg Asp Ile Asn Arg Phe Ser Val Gln Lys Asp 
625                 630                 635                 640 


Phe Ile Asn Arg Asn Leu Val Asp Thr Arg Tyr Ala Thr Arg Gly Leu 
                645                 650                 655     


Met Asn Leu Leu Arg Ser Tyr Phe Arg Val Asn Asn Leu Asp Val Lys 
            660                 665                 670         


Val Lys Ser Ile Asn Gly Gly Phe Thr Ser Phe Leu Arg Arg Lys Trp 
        675                 680                 685             


Lys Phe Lys Lys Glu Arg Asn Lys Gly Tyr Lys His His Ala Glu Asp 
    690                 695                 700                 


Ala Leu Ile Ile Ala Asn Ala Asp Phe Ile Phe Lys Glu Trp Lys Lys 
705                 710                 715                 720 


Leu Asp Lys Ala Lys Lys Val Met Glu Asn Gln Met Phe Glu Glu Lys 
                725                 730                 735     


Gln Ala Glu Ser Met Pro Glu Ile Glu Thr Glu Gln Glu Tyr Lys Glu 
            740                 745                 750         


Ile Phe Ile Thr Pro His Gln Ile Lys His Ile Lys Asp Phe Lys Asp 
        755                 760                 765             


Tyr Lys Tyr Ser His Arg Val Asp Lys Lys Pro Asn Arg Glu Leu Ile 
    770                 775                 780                 


Asn Asp Thr Leu Tyr Ser Thr Arg Lys Asp Asp Lys Gly Asn Thr Leu 
785                 790                 795                 800 


Ile Val Asn Asn Leu Asn Gly Leu Tyr Asp Lys Asp Asn Asp Lys Leu 
                805                 810                 815     


Lys Lys Leu Ile Asn Lys Ser Pro Glu Lys Leu Leu Met Tyr His His 
            820                 825                 830         


Asp Pro Gln Thr Tyr Gln Lys Leu Lys Leu Ile Met Glu Gln Tyr Gly 
        835                 840                 845             


Asp Glu Lys Asn Pro Leu Tyr Lys Tyr Tyr Glu Glu Thr Gly Asn Tyr 
    850                 855                 860                 


Leu Thr Lys Tyr Ser Lys Lys Asp Asn Gly Pro Val Ile Lys Lys Ile 
865                 870                 875                 880 


Lys Tyr Tyr Gly Asn Lys Leu Asn Ala His Leu Asp Ile Thr Asp Asp 
                885                 890                 895     


Tyr Pro Asn Ser Arg Asn Lys Val Val Lys Leu Ser Leu Lys Pro Tyr 
            900                 905                 910         


Arg Phe Asp Val Tyr Leu Asp Asn Gly Val Tyr Lys Phe Val Thr Val 
        915                 920                 925             


Lys Asn Leu Asp Val Ile Lys Lys Glu Asn Tyr Tyr Glu Val Asn Ser 
    930                 935                 940                 


Lys Cys Tyr Glu Glu Ala Lys Lys Leu Lys Lys Ile Ser Asn Gln Ala 
945                 950                 955                 960 


Glu Phe Ile Ala Ser Phe Tyr Asn Asn Asp Leu Ile Lys Ile Asn Gly 
                965                 970                 975     


Glu Leu Tyr Arg Val Ile Gly Val Asn Asn Asp Leu Leu Asn Arg Ile 
            980                 985                 990         


Glu Val Asn Met Ile Asp Ile Thr  Tyr Arg Glu Tyr Leu  Glu Asn Met 
        995                 1000                 1005             


Asn Asp  Lys Arg Pro Pro Arg  Ile Ile Lys Thr Ile  Ala Ser Lys 
    1010                 1015                 1020             


Thr Gln  Ser Ile Lys Lys Tyr  Ser Thr Asp Ile Leu  Gly Asn Leu 
    1025                 1030                 1035             


Tyr Glu  Val Lys Ser Lys Lys  His Pro Gln Ile Ile  Lys Lys Gly 
    1040                 1045                 1050             


<210>  7
<211>  3159
<212>  DNA
<213>  Staphylococcus aureus

<400>  7
atgaaaagga actacattct ggggctggac atcgggatta caagcgtggg gtatgggatt       60

attgactatg aaacaaggga cgtgatcgac gcaggcgtca gactgttcaa ggaggccaac      120

gtggaaaaca atgagggacg gagaagcaag aggggagcca ggcgcctgaa acgacggaga      180

aggcacagaa tccagagggt gaagaaactg ctgttcgatt acaacctgct gaccgaccat      240

tctgagctga gtggaattaa tccttatgaa gccagggtga aaggcctgag tcagaagctg      300

tcagaggaag agttttccgc agctctgctg cacctggcta agcgccgagg agtgcataac      360

gtcaatgagg tggaagagga caccggcaac gagctgtcta caaaggaaca gatctcacgc      420

aatagcaaag ctctggaaga gaagtatgtc gcagagctgc agctggaacg gctgaagaaa      480

gatggcgagg tgagagggtc aattaatagg ttcaagacaa gcgactacgt caaagaagcc      540

aagcagctgc tgaaagtgca gaaggcttac caccagctgg atcagagctt catcgatact      600

tatatcgacc tgctggagac tcggagaacc tactatgagg gaccaggaga agggagcccc      660

ttcggatgga aagacatcaa ggaatggtac gagatgctga tgggacattg cacctatttt      720

ccagaagagc tgagaagcgt caagtacgct tataacgcag atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gaaaacgaga aactggaata ctatgagaag      840

ttccagatca tcgaaaacgt gtttaagcag aagaaaaagc ctacactgaa acagattgct      900

aaggagatcc tggtcaacga agaggacatc aagggctacc gggtgacaag cactggaaaa      960

ccagagttca ccaatctgaa agtgtatcac gatattaagg acatcacagc acggaaagaa     1020

atcattgaga acgccgaact gctggatcag attgctaaga tcctgactat ctaccagagc     1080

tccgaggaca tccaggaaga gctgactaac ctgaacagcg agctgaccca ggaagagatc     1140

gaacagatta gtaatctgaa ggggtacacc ggaacacaca acctgtccct gaaagctatc     1200

aatctgattc tggatgagct gtggcataca aacgacaatc agattgcaat ctttaaccgg     1260

ctgaagctgg tcccaaaaaa ggtggacctg agtcagcaga aagagatccc aaccacactg     1320

gtggacgatt tcattctgtc acccgtggtc aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcattatcga gctggctagg     1440

gagaagaaca gcaaggacgc acagaagatg atcaatgaga tgcagaaacg aaaccggcag     1500

accaatgaac gcattgaaga gattatccga actaccggga aagagaacgc aaagtacctg     1560

attgaaaaaa tcaagctgca cgatatgcag gagggaaagt gtctgtattc tctggaggcc     1620

atccccctgg aggacctgct gaacaatcca ttcaactacg aggtcgatca tattatcccc     1680

agaagcgtgt ccttcgacaa ttcctttaac aacaaggtgc tggtcaagca ggaagagaac     1740

tctaaaaagg gcaataggac tcctttccag tacctgtcta gttcagattc caagatctct     1800

tacgaaacct ttaaaaagca cattctgaat ctggccaaag gaaagggccg catcagcaag     1860

accaaaaagg agtacctgct ggaagagcgg gacatcaaca gattctccgt ccagaaggat     1920

tttattaacc ggaatctggt ggacacaaga tacgctactc gcggcctgat gaatctgctg     1980

cgatcctatt tccgggtgaa caatctggat gtgaaagtca agtccatcaa cggcgggttc     2040

acatcttttc tgaggcgcaa atggaagttt aaaaaggagc gcaacaaagg gtacaagcac     2100

catgccgaag atgctctgat tatcgcaaat gccgacttca tctttaagga gtggaaaaag     2160

ctggacaaag ccaagaaagt gatggagaac cagatgttcg aagagaagca ggccgaatct     2220

atgcccgaaa tcgagacaga acaggagtac aaggagattt tcatcactcc tcaccagatc     2280

aagcatatca aggatttcaa ggactacaag tactctcacc gggtggataa aaagcccaac     2340

agagagctga tcaatgacac cctgtatagt acaagaaaag acgataaggg gaataccctg     2400

attgtgaaca atctgaacgg actgtacgac aaagataatg acaagctgaa aaagctgatc     2460

aacaaaagtc ccgagaagct gctgatgtac caccatgatc ctcagacata tcagaaactg     2520

aagctgatta tggagcagta cggcgacgag aagaacccac tgtataagta ctatgaagag     2580

actgggaact acctgaccaa gtatagcaaa aaggataatg gccccgtgat caagaagatc     2640

aagtactatg ggaacaagct gaatgcccat ctggacatca cagacgatta ccctaacagt     2700

cgcaacaagg tggtcaagct gtcactgaag ccatacagat tcgatgtcta tctggacaac     2760

ggcgtgtata aatttgtgac tgtcaagaat ctggatgtca tcaaaaagga gaactactat     2820

gaagtgaata gcaagtgcta cgaagaggct aaaaagctga aaaagattag caaccaggca     2880

gagttcatcg cctcctttta caacaacgac ctgattaaga tcaatggcga actgtatagg     2940

gtcatcgggg tgaacaatga tctgctgaac cgcattgaag tgaatatgat tgacatcact     3000

taccgagagt atctggaaaa catgaatgat aagcgccccc ctcgaattat caaaacaatt     3060

gcctctaaga ctcagagtat caaaaagtac tcaaccgaca ttctgggaaa cctgtatgag     3120

gtgaagagca aaaagcaccc tcagattatc aaaaagggc                            3159


<210>  8
<211>  3159
<212>  DNA
<213>  Staphylococcus aureus

<400>  8
atgaagcgga actacatcct gggcctggac atcggcatca ccagcgtggg ctacggcatc       60

atcgactacg agacacggga cgtgatcgat gccggcgtgc ggctgttcaa agaggccaac      120

gtggaaaaca acgagggcag gcggagcaag agaggcgcca gaaggctgaa gcggcggagg      180

cggcatagaa tccagagagt gaagaagctg ctgttcgact acaacctgct gaccgaccac      240

agcgagctga gcggcatcaa cccctacgag gccagagtga agggcctgag ccagaagctg      300

agcgaggaag agttctctgc cgccctgctg cacctggcca agagaagagg cgtgcacaac      360

gtgaacgagg tggaagagga caccggcaac gagctgtcca ccaaagagca gatcagccgg      420

aacagcaagg ccctggaaga gaaatacgtg gccgaactgc agctggaacg gctgaagaaa      480

gacggcgaag tgcggggcag catcaacaga ttcaagacca gcgactacgt gaaagaagcc      540

aaacagctgc tgaaggtgca gaaggcctac caccagctgg accagagctt catcgacacc      600

tacatcgacc tgctggaaac ccggcggacc tactatgagg gacctggcga gggcagcccc      660

ttcggctgga aggacatcaa agaatggtac gagatgctga tgggccactg cacctacttc      720

cccgaggaac tgcggagcgt gaagtacgcc tacaacgccg acctgtacaa cgccctgaac      780

gacctgaaca atctcgtgat caccagggac gagaacgaga agctggaata ttacgagaag      840

ttccagatca tcgagaacgt gttcaagcag aagaagaagc ccaccctgaa gcagatcgcc      900

aaagaaatcc tcgtgaacga agaggatatt aagggctaca gagtgaccag caccggcaag      960

cccgagttca ccaacctgaa ggtgtaccac gacatcaagg acattaccgc ccggaaagag     1020

attattgaga acgccgagct gctggatcag attgccaaga tcctgaccat ctaccagagc     1080

agcgaggaca tccaggaaga actgaccaat ctgaactccg agctgaccca ggaagagatc     1140

gagcagatct ctaatctgaa gggctatacc ggcacccaca acctgagcct gaaggccatc     1200

aacctgatcc tggacgagct gtggcacacc aacgacaacc agatcgctat cttcaaccgg     1260

ctgaagctgg tgcccaagaa ggtggacctg tcccagcaga aagagatccc caccaccctg     1320

gtggacgact tcatcctgag ccccgtcgtg aagagaagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaacgaca tcattatcga gctggcccgc     1440

gagaagaact ccaaggacgc ccagaaaatg atcaacgaga tgcagaagcg gaaccggcag     1500

accaacgagc ggatcgagga aatcatccgg accaccggca aagagaacgc caagtacctg     1560

atcgagaaga tcaagctgca cgacatgcag gaaggcaagt gcctgtacag cctggaagcc     1620

atccctctgg aagatctgct gaacaacccc ttcaactatg aggtggacca catcatcccc     1680

agaagcgtgt ccttcgacaa cagcttcaac aacaaggtgc tcgtgaagca ggaagaaaac     1740

agcaagaagg gcaaccggac cccattccag tacctgagca gcagcgacag caagatcagc     1800

tacgaaacct tcaagaagca catcctgaat ctggccaagg gcaagggcag aatcagcaag     1860

accaagaaag agtatctgct ggaagaacgg gacatcaaca ggttctccgt gcagaaagac     1920

ttcatcaacc ggaacctggt ggataccaga tacgccacca gaggcctgat gaacctgctg     1980

cggagctact tcagagtgaa caacctggac gtgaaagtga agtccatcaa tggcggcttc     2040

accagctttc tgcggcggaa gtggaagttt aagaaagagc ggaacaaggg gtacaagcac     2100

cacgccgagg acgccctgat cattgccaac gccgatttca tcttcaaaga gtggaagaaa     2160

ctggacaagg ccaaaaaagt gatggaaaac cagatgttcg aggaaaagca ggccgagagc     2220

atgcccgaga tcgaaaccga gcaggagtac aaagagatct tcatcacccc ccaccagatc     2280

aagcacatta aggacttcaa ggactacaag tacagccacc gggtggacaa gaagcctaat     2340

agagagctga ttaacgacac cctgtactcc acccggaagg acgacaaggg caacaccctg     2400

atcgtgaaca atctgaacgg cctgtacgac aaggacaatg acaagctgaa aaagctgatc     2460

aacaagagcc ccgaaaagct gctgatgtac caccacgacc cccagaccta ccagaaactg     2520

aagctgatta tggaacagta cggcgacgag aagaatcccc tgtacaagta ctacgaggaa     2580

accgggaact acctgaccaa gtactccaaa aaggacaacg gccccgtgat caagaagatt     2640

aagtattacg gcaacaaact gaacgcccat ctggacatca ccgacgacta ccccaacagc     2700

agaaacaagg tcgtgaagct gtccctgaag ccctacagat tcgacgtgta cctggacaat     2760

ggcgtgtaca agttcgtgac cgtgaagaat ctggatgtga tcaaaaaaga aaactactac     2820

gaagtgaata gcaagtgcta tgaggaagct aagaagctga agaagatcag caaccaggcc     2880

gagtttatcg cctccttcta caacaacgat ctgatcaaga tcaacggcga gctgtataga     2940

gtgatcggcg tgaacaacga cctgctgaac cggatcgaag tgaacatgat cgacatcacc     3000

taccgcgagt acctggaaaa catgaacgac aagaggcccc ccaggatcat taagacaatc     3060

gcctccaaga cccagagcat taagaagtac agcacagaca ttctgggcaa cctgtatgaa     3120

gtgaaatcta agaagcaccc tcagatcatc aaaaagggc                            3159


<210>  9
<211>  3159
<212>  DNA
<213>  Staphylococcus aureus

<400>  9
atgaagcgca actacatcct cggactggac atcggcatta cctccgtggg atacggcatc       60

atcgattacg aaactaggga tgtgatcgac gctggagtca ggctgttcaa agaggcgaac      120

gtggagaaca acgaggggcg gcgctcaaag aggggggccc gccggctgaa gcgccgccgc      180

agacatagaa tccagcgcgt gaagaagctg ctgttcgact acaaccttct gaccgaccac      240

tccgaacttt ccggcatcaa cccatatgag gctagagtga agggattgtc ccaaaagctg      300

tccgaggaag agttctccgc cgcgttgctc cacctcgcca agcgcagggg agtgcacaat      360

gtgaacgaag tggaagaaga taccggaaac gagctgtcca ccaaggagca gatcagccgg      420

aactccaagg ccctggaaga gaaatacgtg gcggaactgc aactggagcg gctgaagaaa      480

gacggagaag tgcgcggctc gatcaaccgc ttcaagacct cggactacgt gaaggaggcc      540

aagcagctcc tgaaagtgca aaaggcctat caccaacttg accagtcctt tatcgatacc      600

tacatcgatc tgctcgagac tcggcggact tactacgagg gtccagggga gggctcccca      660

tttggttgga aggatattaa ggagtggtac gaaatgctga tgggacactg cacatacttc      720

cctgaggagc tgcggagcgt gaaatacgca tacaacgcag acctgtacaa cgcgctgaac      780

gacctgaaca atctcgtgat cacccgggac gagaacgaaa agctcgagta ttacgaaaag      840

ttccagatta ttgagaacgt gttcaaacag aagaagaagc cgacactgaa gcagattgcc      900

aaggaaatcc tcgtgaacga agaggacatc aagggctatc gagtgacctc aacgggaaag      960

ccggagttca ccaatctgaa ggtctaccac gacatcaaag acattaccgc ccggaaggag     1020

atcattgaga acgcggagct gttggaccag attgcgaaga ttctgaccat ctaccaatcc     1080

tccgaggata ttcaggaaga actcaccaac ctcaacagcg aactgaccca ggaggagata     1140

gagcaaatct ccaacctgaa gggctacacc ggaactcata acctgagcct gaaggccatc     1200

aacttgatcc tggacgagct gtggcacacc aacgataacc agatcgctat tttcaatcgg     1260

ctgaagctgg tccccaagaa agtggacctc tcacaacaaa aggagatccc tactaccctt     1320

gtggacgatt tcattctgtc ccccgtggtc aagagaagct tcatacagtc aatcaaagtg     1380

atcaatgcca ttatcaagaa atacggtctg cccaacgaca ttatcattga gctcgcccgc     1440

gagaagaact cgaaggacgc ccagaagatg attaacgaaa tgcagaagag gaaccgacag     1500

actaacgaac ggatcgaaga aatcatccgg accaccggga aggaaaacgc gaagtacctg     1560

atcgaaaaga tcaagctcca tgacatgcag gaaggaaagt gtctgtactc gctggaggcc     1620

attccgctgg aggacttgct gaacaaccct tttaactacg aagtggatca tatcattccg     1680

aggagcgtgt cattcgacaa ttccttcaac aacaaggtcc tcgtgaagca ggaggaaaac     1740

tcgaagaagg gaaaccgcac gccgttccag tacctgagca gcagcgactc caagatttcc     1800

tacgaaacct tcaagaagca catcctcaac ctggcaaagg ggaagggtcg catctccaag     1860

accaagaagg aatatctgct ggaagaaaga gacatcaaca gattctccgt gcaaaaggac     1920

ttcatcaacc gcaacctcgt ggatactaga tacgctactc ggggtctgat gaacctcctg     1980

agaagctact ttagagtgaa caatctggac gtgaaggtca agtcgattaa cggaggtttc     2040

acctccttcc tgcggcgcaa gtggaagttc aagaaggaac ggaacaaggg ctacaagcac     2100

cacgccgagg acgccctgat cattgccaac gccgacttca tcttcaaaga atggaagaaa     2160

cttgacaagg ctaagaaggt catggaaaac cagatgttcg aagaaaagca ggccgagtct     2220

atgcctgaaa tcgagactga acaggagtac aaggaaatct ttattacgcc acaccagatc     2280

aaacacatca aggatttcaa ggattacaag tactcacatc gcgtggacaa aaagccgaac     2340

agggaactga tcaacgacac cctctactcc acccggaagg atgacaaagg gaataccctc     2400

atcgtcaaca accttaacgg cctgtacgac aaggacaacg ataagctgaa gaagctcatt     2460

aacaagtcgc ccgaaaagtt gctgatgtac caccacgacc ctcagactta ccagaagctc     2520

aagctgatca tggagcagta tggggacgag aaaaacccgt tgtacaagta ctacgaagaa     2580

actgggaatt atctgactaa gtactccaag aaagataacg gccccgtgat taagaagatt     2640

aagtactacg gcaacaagct gaacgcccat ctggacatca ccgatgacta ccctaattcc     2700

cgcaacaagg tcgtcaagct gagcctcaag ccctaccggt ttgatgtgta ccttgacaat     2760

ggagtgtaca agttcgtgac tgtgaagaac cttgacgtga tcaagaagga gaactactac     2820

gaagtcaact ccaagtgcta cgaggaagca aagaagttga agaagatctc gaaccaggcc     2880

gagttcattg cctccttcta taacaacgac ctgattaaga tcaacggcga actgtaccgc     2940

gtcattggcg tgaacaacga tctcctgaac cgcatcgaag tgaacatgat cgacatcact     3000

taccgggaat acctggagaa tatgaacgac aagcgcccgc cccggatcat taagactatc     3060

gcctcaaaga cccagtcgat caagaagtac agcaccgaca tcctgggcaa cctgtacgag     3120

gtcaaatcga agaagcaccc ccagatcatc aagaaggga                            3159


<210>  10
<211>  3159
<212>  DNA
<213>  Staphylococcus aureus

<400>  10
atgaaaagga actacattct ggggctggcc atcgggatta caagcgtggg gtatgggatt       60

attgactatg aaacaaggga cgtgatcgac gcaggcgtca gactgttcaa ggaggccaac      120

gtggaaaaca atgagggacg gagaagcaag aggggagcca ggcgcctgaa acgacggaga      180

aggcacagaa tccagagggt gaagaaactg ctgttcgatt acaacctgct gaccgaccat      240

tctgagctga gtggaattaa tccttatgaa gccagggtga aaggcctgag tcagaagctg      300

tcagaggaag agttttccgc agctctgctg cacctggcta agcgccgagg agtgcataac      360

gtcaatgagg tggaagagga caccggcaac gagctgtcta caaaggaaca gatctcacgc      420

aatagcaaag ctctggaaga gaagtatgtc gcagagctgc agctggaacg gctgaagaaa      480

gatggcgagg tgagagggtc aattaatagg ttcaagacaa gcgactacgt caaagaagcc      540

aagcagctgc tgaaagtgca gaaggcttac caccagctgg atcagagctt catcgatact      600

tatatcgacc tgctggagac tcggagaacc tactatgagg gaccaggaga agggagcccc      660

ttcggatgga aagacatcaa ggaatggtac gagatgctga tgggacattg cacctatttt      720

ccagaagagc tgagaagcgt caagtacgct tataacgcag atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gaaaacgaga aactggaata ctatgagaag      840

ttccagatca tcgaaaacgt gtttaagcag aagaaaaagc ctacactgaa acagattgct      900

aaggagatcc tggtcaacga agaggacatc aagggctacc gggtgacaag cactggaaaa      960

ccagagttca ccaatctgaa agtgtatcac gatattaagg acatcacagc acggaaagaa     1020

atcattgaga acgccgaact gctggatcag attgctaaga tcctgactat ctaccagagc     1080

tccgaggaca tccaggaaga gctgactaac ctgaacagcg agctgaccca ggaagagatc     1140

gaacagatta gtaatctgaa ggggtacacc ggaacacaca acctgtccct gaaagctatc     1200

aatctgattc tggatgagct gtggcataca aacgacaatc agattgcaat ctttaaccgg     1260

ctgaagctgg tcccaaaaaa ggtggacctg agtcagcaga aagagatccc aaccacactg     1320

gtggacgatt tcattctgtc acccgtggtc aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcattatcga gctggctagg     1440

gagaagaaca gcaaggacgc acagaagatg atcaatgaga tgcagaaacg aaaccggcag     1500

accaatgaac gcattgaaga gattatccga actaccggga aagagaacgc aaagtacctg     1560

attgaaaaaa tcaagctgca cgatatgcag gagggaaagt gtctgtattc tctggaggcc     1620

atccccctgg aggacctgct gaacaatcca ttcaactacg aggtcgatca tattatcccc     1680

agaagcgtgt ccttcgacaa ttcctttaac aacaaggtgc tggtcaagca ggaagagaac     1740

tctaaaaagg gcaataggac tcctttccag tacctgtcta gttcagattc caagatctct     1800

tacgaaacct ttaaaaagca cattctgaat ctggccaaag gaaagggccg catcagcaag     1860

accaaaaagg agtacctgct ggaagagcgg gacatcaaca gattctccgt ccagaaggat     1920

tttattaacc ggaatctggt ggacacaaga tacgctactc gcggcctgat gaatctgctg     1980

cgatcctatt tccgggtgaa caatctggat gtgaaagtca agtccatcaa cggcgggttc     2040

acatcttttc tgaggcgcaa atggaagttt aaaaaggagc gcaacaaagg gtacaagcac     2100

catgccgaag atgctctgat tatcgcaaat gccgacttca tctttaagga gtggaaaaag     2160

ctggacaaag ccaagaaagt gatggagaac cagatgttcg aagagaagca ggccgaatct     2220

atgcccgaaa tcgagacaga acaggagtac aaggagattt tcatcactcc tcaccagatc     2280

aagcatatca aggatttcaa ggactacaag tactctcacc gggtggataa aaagcccaac     2340

agagagctga tcaatgacac cctgtatagt acaagaaaag acgataaggg gaataccctg     2400

attgtgaaca atctgaacgg actgtacgac aaagataatg acaagctgaa aaagctgatc     2460

aacaaaagtc ccgagaagct gctgatgtac caccatgatc ctcagacata tcagaaactg     2520

aagctgatta tggagcagta cggcgacgag aagaacccac tgtataagta ctatgaagag     2580

actgggaact acctgaccaa gtatagcaaa aaggataatg gccccgtgat caagaagatc     2640

aagtactatg ggaacaagct gaatgcccat ctggacatca cagacgatta ccctaacagt     2700

cgcaacaagg tggtcaagct gtcactgaag ccatacagat tcgatgtcta tctggacaac     2760

ggcgtgtata aatttgtgac tgtcaagaat ctggatgtca tcaaaaagga gaactactat     2820

gaagtgaata gcaagtgcta cgaagaggct aaaaagctga aaaagattag caaccaggca     2880

gagttcatcg cctcctttta caacaacgac ctgattaaga tcaatggcga actgtatagg     2940

gtcatcgggg tgaacaatga tctgctgaac cgcattgaag tgaatatgat tgacatcact     3000

taccgagagt atctggaaaa catgaatgat aagcgccccc ctcgaattat caaaacaatt     3060

gcctctaaga ctcagagtat caaaaagtac tcaaccgaca ttctgggaaa cctgtatgag     3120

gtgaagagca aaaagcaccc tcagattatc aaaaagggc                            3159


<210>  11
<211>  3159
<212>  DNA
<213>  Staphylococcus aureus

<400>  11
atgaaaagga actacattct ggggctggac atcgggatta caagcgtggg gtatgggatt       60

attgactatg aaacaaggga cgtgatcgac gcaggcgtca gactgttcaa ggaggccaac      120

gtggaaaaca atgagggacg gagaagcaag aggggagcca ggcgcctgaa acgacggaga      180

aggcacagaa tccagagggt gaagaaactg ctgttcgatt acaacctgct gaccgaccat      240

tctgagctga gtggaattaa tccttatgaa gccagggtga aaggcctgag tcagaagctg      300

tcagaggaag agttttccgc agctctgctg cacctggcta agcgccgagg agtgcataac      360

gtcaatgagg tggaagagga caccggcaac gagctgtcta caaaggaaca gatctcacgc      420

aatagcaaag ctctggaaga gaagtatgtc gcagagctgc agctggaacg gctgaagaaa      480

gatggcgagg tgagagggtc aattaatagg ttcaagacaa gcgactacgt caaagaagcc      540

aagcagctgc tgaaagtgca gaaggcttac caccagctgg atcagagctt catcgatact      600

tatatcgacc tgctggagac tcggagaacc tactatgagg gaccaggaga agggagcccc      660

ttcggatgga aagacatcaa ggaatggtac gagatgctga tgggacattg cacctatttt      720

ccagaagagc tgagaagcgt caagtacgct tataacgcag atctgtacaa cgccctgaat      780

gacctgaaca acctggtcat caccagggat gaaaacgaga aactggaata ctatgagaag      840

ttccagatca tcgaaaacgt gtttaagcag aagaaaaagc ctacactgaa acagattgct      900

aaggagatcc tggtcaacga agaggacatc aagggctacc gggtgacaag cactggaaaa      960

ccagagttca ccaatctgaa agtgtatcac gatattaagg acatcacagc acggaaagaa     1020

atcattgaga acgccgaact gctggatcag attgctaaga tcctgactat ctaccagagc     1080

tccgaggaca tccaggaaga gctgactaac ctgaacagcg agctgaccca ggaagagatc     1140

gaacagatta gtaatctgaa ggggtacacc ggaacacaca acctgtccct gaaagctatc     1200

aatctgattc tggatgagct gtggcataca aacgacaatc agattgcaat ctttaaccgg     1260

ctgaagctgg tcccaaaaaa ggtggacctg agtcagcaga aagagatccc aaccacactg     1320

gtggacgatt tcattctgtc acccgtggtc aagcggagct tcatccagag catcaaagtg     1380

atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcattatcga gctggctagg     1440

gagaagaaca gcaaggacgc acagaagatg atcaatgaga tgcagaaacg aaaccggcag     1500

accaatgaac gcattgaaga gattatccga actaccggga aagagaacgc aaagtacctg     1560

attgaaaaaa tcaagctgca cgatatgcag gagggaaagt gtctgtattc tctggaggcc     1620

atccccctgg aggacctgct gaacaatcca ttcaactacg aggtcgatca tattatcccc     1680

agaagcgtgt ccttcgacaa ttcctttaac aacaaggtgc tggtcaagca ggaagaggcc     1740

tctaaaaagg gcaataggac tcctttccag tacctgtcta gttcagattc caagatctct     1800

tacgaaacct ttaaaaagca cattctgaat ctggccaaag gaaagggccg catcagcaag     1860

accaaaaagg agtacctgct ggaagagcgg gacatcaaca gattctccgt ccagaaggat     1920

tttattaacc ggaatctggt ggacacaaga tacgctactc gcggcctgat gaatctgctg     1980

cgatcctatt tccgggtgaa caatctggat gtgaaagtca agtccatcaa cggcgggttc     2040

acatcttttc tgaggcgcaa atggaagttt aaaaaggagc gcaacaaagg gtacaagcac     2100

catgccgaag atgctctgat tatcgcaaat gccgacttca tctttaagga gtggaaaaag     2160

ctggacaaag ccaagaaagt gatggagaac cagatgttcg aagagaagca ggccgaatct     2220

atgcccgaaa tcgagacaga acaggagtac aaggagattt tcatcactcc tcaccagatc     2280

aagcatatca aggatttcaa ggactacaag tactctcacc gggtggataa aaagcccaac     2340

agagagctga tcaatgacac cctgtatagt acaagaaaag acgataaggg gaataccctg     2400

attgtgaaca atctgaacgg actgtacgac aaagataatg acaagctgaa aaagctgatc     2460

aacaaaagtc ccgagaagct gctgatgtac caccatgatc ctcagacata tcagaaactg     2520

aagctgatta tggagcagta cggcgacgag aagaacccac tgtataagta ctatgaagag     2580

actgggaact acctgaccaa gtatagcaaa aaggataatg gccccgtgat caagaagatc     2640

aagtactatg ggaacaagct gaatgcccat ctggacatca cagacgatta ccctaacagt     2700

cgcaacaagg tggtcaagct gtcactgaag ccatacagat tcgatgtcta tctggacaac     2760

ggcgtgtata aatttgtgac tgtcaagaat ctggatgtca tcaaaaagga gaactactat     2820

gaagtgaata gcaagtgcta cgaagaggct aaaaagctga aaaagattag caaccaggca     2880

gagttcatcg cctcctttta caacaacgac ctgattaaga tcaatggcga actgtatagg     2940

gtcatcgggg tgaacaatga tctgctgaac cgcattgaag tgaatatgat tgacatcact     3000

taccgagagt atctggaaaa catgaatgat aagcgccccc ctcgaattat caaaacaatt     3060

gcctctaaga ctcagagtat caaaaagtac tcaaccgaca ttctgggaaa cctgtatgag     3120

gtgaagagca aaaagcaccc tcagattatc aaaaagggc                            3159


<210>  12
<211>  1082
<212>  PRT
<213>  Neisseria meningitidis

<400>  12

Met Ala Ala Phe Lys Pro Asn Pro Ile Asn Tyr Ile Leu Gly Leu Asp 
1               5                   10                  15      


Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val Glu Ile Asp Glu Asp 
            20                  25                  30          


Glu Asn Pro Ile Cys Leu Ile Asp Leu Gly Val Arg Val Phe Glu Arg 
        35                  40                  45              


Ala Glu Val Pro Lys Thr Gly Asp Ser Leu Ala Met Ala Arg Arg Leu 
    50                  55                  60                  


Ala Arg Ser Val Arg Arg Leu Thr Arg Arg Arg Ala His Arg Leu Leu 
65                  70                  75                  80  


Arg Ala Arg Arg Leu Leu Lys Arg Glu Gly Val Leu Gln Ala Ala Asp 
                85                  90                  95      


Phe Asp Glu Asn Gly Leu Ile Lys Ser Leu Pro Asn Thr Pro Trp Gln 
            100                 105                 110         


Leu Arg Ala Ala Ala Leu Asp Arg Lys Leu Thr Pro Leu Glu Trp Ser 
        115                 120                 125             


Ala Val Leu Leu His Leu Ile Lys His Arg Gly Tyr Leu Ser Gln Arg 
    130                 135                 140                 


Lys Asn Glu Gly Glu Thr Ala Asp Lys Glu Leu Gly Ala Leu Leu Lys 
145                 150                 155                 160 


Gly Val Ala Asp Asn Ala His Ala Leu Gln Thr Gly Asp Phe Arg Thr 
                165                 170                 175     


Pro Ala Glu Leu Ala Leu Asn Lys Phe Glu Lys Glu Ser Gly His Ile 
            180                 185                 190         


Arg Asn Gln Arg Gly Asp Tyr Ser His Thr Phe Ser Arg Lys Asp Leu 
        195                 200                 205             


Gln Ala Glu Leu Ile Leu Leu Phe Glu Lys Gln Lys Glu Phe Gly Asn 
    210                 215                 220                 


Pro His Val Ser Gly Gly Leu Lys Glu Gly Ile Glu Thr Leu Leu Met 
225                 230                 235                 240 


Thr Gln Arg Pro Ala Leu Ser Gly Asp Ala Val Gln Lys Met Leu Gly 
                245                 250                 255     


His Cys Thr Phe Glu Pro Ala Glu Pro Lys Ala Ala Lys Asn Thr Tyr 
            260                 265                 270         


Thr Ala Glu Arg Phe Ile Trp Leu Thr Lys Leu Asn Asn Leu Arg Ile 
        275                 280                 285             


Leu Glu Gln Gly Ser Glu Arg Pro Leu Thr Asp Thr Glu Arg Ala Thr 
    290                 295                 300                 


Leu Met Asp Glu Pro Tyr Arg Lys Ser Lys Leu Thr Tyr Ala Gln Ala 
305                 310                 315                 320 


Arg Lys Leu Leu Gly Leu Glu Asp Thr Ala Phe Phe Lys Gly Leu Arg 
                325                 330                 335     


Tyr Gly Lys Asp Asn Ala Glu Ala Ser Thr Leu Met Glu Met Lys Ala 
            340                 345                 350         


Tyr His Ala Ile Ser Arg Ala Leu Glu Lys Glu Gly Leu Lys Asp Lys 
        355                 360                 365             


Lys Ser Pro Leu Asn Leu Ser Pro Glu Leu Gln Asp Glu Ile Gly Thr 
    370                 375                 380                 


Ala Phe Ser Leu Phe Lys Thr Asp Glu Asp Ile Thr Gly Arg Leu Lys 
385                 390                 395                 400 


Asp Arg Ile Gln Pro Glu Ile Leu Glu Ala Leu Leu Lys His Ile Ser 
                405                 410                 415     


Phe Asp Lys Phe Val Gln Ile Ser Leu Lys Ala Leu Arg Arg Ile Val 
            420                 425                 430         


Pro Leu Met Glu Gln Gly Lys Arg Tyr Asp Glu Ala Cys Ala Glu Ile 
        435                 440                 445             


Tyr Gly Asp His Tyr Gly Lys Lys Asn Thr Glu Glu Lys Ile Tyr Leu 
    450                 455                 460                 


Pro Pro Ile Pro Ala Asp Glu Ile Arg Asn Pro Val Val Leu Arg Ala 
465                 470                 475                 480 


Leu Ser Gln Ala Arg Lys Val Ile Asn Gly Val Val Arg Arg Tyr Gly 
                485                 490                 495     


Ser Pro Ala Arg Ile His Ile Glu Thr Ala Arg Glu Val Gly Lys Ser 
            500                 505                 510         


Phe Lys Asp Arg Lys Glu Ile Glu Lys Arg Gln Glu Glu Asn Arg Lys 
        515                 520                 525             


Asp Arg Glu Lys Ala Ala Ala Lys Phe Arg Glu Tyr Phe Pro Asn Phe 
    530                 535                 540                 


Val Gly Glu Pro Lys Ser Lys Asp Ile Leu Lys Leu Arg Leu Tyr Glu 
545                 550                 555                 560 


Gln Gln His Gly Lys Cys Leu Tyr Ser Gly Lys Glu Ile Asn Leu Gly 
                565                 570                 575     


Arg Leu Asn Glu Lys Gly Tyr Val Glu Ile Asp His Ala Leu Pro Phe 
            580                 585                 590         


Ser Arg Thr Trp Asp Asp Ser Phe Asn Asn Lys Val Leu Val Leu Gly 
        595                 600                 605             


Ser Glu Asn Gln Asn Lys Gly Asn Gln Thr Pro Tyr Glu Tyr Phe Asn 
    610                 615                 620                 


Gly Lys Asp Asn Ser Arg Glu Trp Gln Glu Phe Lys Ala Arg Val Glu 
625                 630                 635                 640 


Thr Ser Arg Phe Pro Arg Ser Lys Lys Gln Arg Ile Leu Leu Gln Lys 
                645                 650                 655     


Phe Asp Glu Asp Gly Phe Lys Glu Arg Asn Leu Asn Asp Thr Arg Tyr 
            660                 665                 670         


Val Asn Arg Phe Leu Cys Gln Phe Val Ala Asp Arg Met Arg Leu Thr 
        675                 680                 685             


Gly Lys Gly Lys Lys Arg Val Phe Ala Ser Asn Gly Gln Ile Thr Asn 
    690                 695                 700                 


Leu Leu Arg Gly Phe Trp Gly Leu Arg Lys Val Arg Ala Glu Asn Asp 
705                 710                 715                 720 


Arg His His Ala Leu Asp Ala Val Val Val Ala Cys Ser Thr Val Ala 
                725                 730                 735     


Met Gln Gln Lys Ile Thr Arg Phe Val Arg Tyr Lys Glu Met Asn Ala 
            740                 745                 750         


Phe Asp Gly Lys Thr Ile Asp Lys Glu Thr Gly Glu Val Leu His Gln 
        755                 760                 765             


Lys Thr His Phe Pro Gln Pro Trp Glu Phe Phe Ala Gln Glu Val Met 
    770                 775                 780                 


Ile Arg Val Phe Gly Lys Pro Asp Gly Lys Pro Glu Phe Glu Glu Ala 
785                 790                 795                 800 


Asp Thr Pro Glu Lys Leu Arg Thr Leu Leu Ala Glu Lys Leu Ser Ser 
                805                 810                 815     


Arg Pro Glu Ala Val His Glu Tyr Val Thr Pro Leu Phe Val Ser Arg 
            820                 825                 830         


Ala Pro Asn Arg Lys Met Ser Gly Gln Gly His Met Glu Thr Val Lys 
        835                 840                 845             


Ser Ala Lys Arg Leu Asp Glu Gly Val Ser Val Leu Arg Val Pro Leu 
    850                 855                 860                 


Thr Gln Leu Lys Leu Lys Asp Leu Glu Lys Met Val Asn Arg Glu Arg 
865                 870                 875                 880 


Glu Pro Lys Leu Tyr Glu Ala Leu Lys Ala Arg Leu Glu Ala His Lys 
                885                 890                 895     


Asp Asp Pro Ala Lys Ala Phe Ala Glu Pro Phe Tyr Lys Tyr Asp Lys 
            900                 905                 910         


Ala Gly Asn Arg Thr Gln Gln Val Lys Ala Val Arg Val Glu Gln Val 
        915                 920                 925             


Gln Lys Thr Gly Val Trp Val Arg Asn His Asn Gly Ile Ala Asp Asn 
    930                 935                 940                 


Ala Thr Met Val Arg Val Asp Val Phe Glu Lys Gly Asp Lys Tyr Tyr 
945                 950                 955                 960 


Leu Val Pro Ile Tyr Ser Trp Gln Val Ala Lys Gly Ile Leu Pro Asp 
                965                 970                 975     


Arg Ala Val Val Gln Gly Lys Asp Glu Glu Asp Trp Gln Leu Ile Asp 
            980                 985                 990         


Asp Ser Phe Asn Phe Lys Phe Ser  Leu His Pro Asn Asp  Leu Val Glu 
        995                 1000                 1005             


Val Ile  Thr Lys Lys Ala Arg  Met Phe Gly Tyr Phe  Ala Ser Cys 
    1010                 1015                 1020             


His Arg  Gly Thr Gly Asn Ile  Asn Ile Arg Ile His  Asp Leu Asp 
    1025                 1030                 1035             


His Lys  Ile Gly Lys Asn Gly  Ile Leu Glu Gly Ile  Gly Val Lys 
    1040                 1045                 1050             


Thr Ala  Leu Ser Phe Gln Lys  Tyr Gln Ile Asp Glu  Leu Gly Lys 
    1055                 1060                 1065             


Glu Ile  Arg Pro Cys Arg Leu  Lys Lys Arg Pro Pro  Val Arg 
    1070                 1075                 1080         


<210>  13
<211>  3249
<212>  DNA
<213>  Neisseria meningitidis


<220>
<221>  misc_feature
<222>  (1)..(3249)
<223>  Exemplary codon optimized Cas9

<400>  13
atggccgcct tcaagcccaa ccccatcaac tacatcctgg gcctggacat cggcatcgcc       60

agcgtgggct gggccatggt ggagatcgac gaggacgaga accccatctg cctgatcgac      120

ctgggtgtgc gcgtgttcga gcgcgctgag gtgcccaaga ctggtgacag tctggctatg      180

gctcgccggc ttgctcgctc tgttcggcgc cttactcgcc ggcgcgctca ccgccttctg      240

cgcgctcgcc gcctgctgaa gcgcgagggt gtgctgcagg ctgccgactt cgacgagaac      300

ggcctgatca agagcctgcc caacactcct tggcagctgc gcgctgccgc tctggaccgc      360

aagctgactc ctctggagtg gagcgccgtg ctgctgcacc tgatcaagca ccgcggctac      420

ctgagccagc gcaagaacga gggcgagacc gccgacaagg agctgggtgc tctgctgaag      480

ggcgtggccg acaacgccca cgccctgcag actggtgact tccgcactcc tgctgagctg      540

gccctgaaca agttcgagaa ggagagcggc cacatccgca accagcgcgg cgactacagc      600

cacaccttca gccgcaagga cctgcaggcc gagctgatcc tgctgttcga gaagcagaag      660

gagttcggca acccccacgt gagcggcggc ctgaaggagg gcatcgagac cctgctgatg      720

acccagcgcc ccgccctgag cggcgacgcc gtgcagaaga tgctgggcca ctgcaccttc      780

gagccagccg agcccaaggc cgccaagaac acctacaccg ccgagcgctt catctggctg      840

accaagctga acaacctgcg catcctggag cagggcagcg agcgccccct gaccgacacc      900

gagcgcgcca ccctgatgga cgagccctac cgcaagagca agctgaccta cgcccaggcc      960

cgcaagctgc tgggtctgga ggacaccgcc ttcttcaagg gcctgcgcta cggcaaggac     1020

aacgccgagg ccagcaccct gatggagatg aaggcctacc acgccatcag ccgcgccctg     1080

gagaaggagg gcctgaagga caagaagagt cctctgaacc tgagccccga gctgcaggac     1140

gagatcggca ccgccttcag cctgttcaag accgacgagg acatcaccgg ccgcctgaag     1200

gaccgcatcc agcccgagat cctggaggcc ctgctgaagc acatcagctt cgacaagttc     1260

gtgcagatca gcctgaaggc cctgcgccgc atcgtgcccc tgatggagca gggcaagcgc     1320

tacgacgagg cctgcgccga gatctacggc gaccactacg gcaagaagaa caccgaggag     1380

aagatctacc tgcctcctat ccccgccgac gagatccgca accccgtggt gctgcgcgcc     1440

ctgagccagg cccgcaaggt gatcaacggc gtggtgcgcc gctacggcag ccccgcccgc     1500

atccacatcg agaccgcccg cgaggtgggc aagagcttca aggaccgcaa ggagatcgag     1560

aagcgccagg aggagaaccg caaggaccgc gagaaggccg ccgccaagtt ccgcgagtac     1620

ttccccaact tcgtgggcga gcccaagagc aaggacatcc tgaagctgcg cctgtacgag     1680

cagcagcacg gcaagtgcct gtacagcggc aaggagatca acctgggccg cctgaacgag     1740

aagggctacg tggagatcga ccacgccctg cccttcagcc gcacctggga cgacagcttc     1800

aacaacaagg tgctggtgct gggcagcgag aaccagaaca agggcaacca gaccccctac     1860

gagtacttca acggcaagga caacagccgc gagtggcagg agttcaaggc ccgcgtggag     1920

accagccgct tcccccgcag caagaagcag cgcatcctgc tgcagaagtt cgacgaggac     1980

ggcttcaagg agcgcaacct gaacgacacc cgctacgtga accgcttcct gtgccagttc     2040

gtggccgacc gcatgcgcct gaccggcaag ggcaagaagc gcgtgttcgc cagcaacggc     2100

cagatcacca acctgctgcg cggcttctgg ggcctgcgca aggtgcgcgc cgagaacgac     2160

cgccaccacg ccctggacgc cgtggtggtg gcctgcagca ccgtggccat gcagcagaag     2220

atcacccgct tcgtgcgcta caaggagatg aacgccttcg acggtaaaac catcgacaag     2280

gagaccggcg aggtgctgca ccagaagacc cacttccccc agccctggga gttcttcgcc     2340

caggaggtga tgatccgcgt gttcggcaag cccgacggca agcccgagtt cgaggaggcc     2400

gacacccccg agaagctgcg caccctgctg gccgagaagc tgagcagccg ccctgaggcc     2460

gtgcacgagt acgtgactcc tctgttcgtg agccgcgccc ccaaccgcaa gatgagcggt     2520

cagggtcaca tggagaccgt gaagagcgcc aagcgcctgg acgagggcgt gagcgtgctg     2580

cgcgtgcccc tgacccagct gaagctgaag gacctggaga agatggtgaa ccgcgagcgc     2640

gagcccaagc tgtacgaggc cctgaaggcc cgcctggagg cccacaagga cgaccccgcc     2700

aaggccttcg ccgagccctt ctacaagtac gacaaggccg gcaaccgcac ccagcaggtg     2760

aaggccgtgc gcgtggagca ggtgcagaag accggcgtgt gggtgcgcaa ccacaacggc     2820

atcgccgaca acgccaccat ggtgcgcgtg gacgtgttcg agaagggcga caagtactac     2880

ctggtgccca tctacagctg gcaggtggcc aagggcatcc tgcccgaccg cgccgtggtg     2940

cagggcaagg acgaggagga ctggcagctg atcgacgaca gcttcaactt caagttcagc     3000

ctgcacccca acgacctggt ggaggtgatc accaagaagg cccgcatgtt cggctacttc     3060

gccagctgcc accgcggcac cggcaacatc aacatccgca tccacgacct ggaccacaag     3120

atcggcaaga acggcatcct ggagggcatc ggcgtgaaga ccgccctgag cttccagaag     3180

taccagatcg acgagctggg caaggagatc cgcccctgcc gcctgaagaa gcgccctcct     3240

gtgcgctaa                                                             3249


<210>  14
<211>  859
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Cas9 consensus sequence derived from Sm, Sp, St, and Li
       Cas9 sequences


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (8)..(18)
<223>  N-terminal RuvC-like domain

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (29)..(31)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (36)..(36)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (54)..(54)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (63)..(63)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (71)..(71)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (75)..(75)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (76)..(76)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (78)..(80)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (84)..(84)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (85)..(85)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (89)..(89)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (90)..(90)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (98)..(98)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (100)..(100)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (106)..(106)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (113)..(113)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (116)..(116)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (125)..(125)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (126)..(126)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (128)..(133)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (135)..(135)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (137)..(137)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (139)..(147)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (153)..(155)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (157)..(157)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (159)..(159)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (161)..(161)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (163)..(163)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (166)..(168)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (170)..(170)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (171)..(171)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (173)..(175)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (177)..(177)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (183)..(183)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (185)..(187)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (189)..(189)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (192)..(195)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (198)..(198)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (199)..(199)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (202)..(202)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (206)..(206)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (207)..(207)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (210)..(210)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (212)..(212)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (213)..(213)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (219)..(219)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (220)..(220)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (222)..(222)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (224)..(224)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (226)..(226)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (236)..(236)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (240)..(240)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (241)..(241)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (244)..(246)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (248)..(248)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (249)..(249)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (250)..(250)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (252)..(254)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (256)..(256)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (257)..(257)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (268)..(268)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (271)..(271)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (273)..(273)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (277)..(277)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (280)..(280)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (281)..(281)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (283)..(283)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (289)..(289)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (290)..(290)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (292)..(294)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (301)..(301)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (308)..(308)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (319)..(322)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (328)..(328)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (329)..(329)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (335)..(337)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (346)..(346)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (347)..(347)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (356)..(361)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (363)..(363)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (370)..(373)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (375)..(375)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (376)..(376)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (379)..(379)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (386)..(390)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (393)..(393)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (395)..(395)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (396)..(396)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (398)..(398)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (400)..(400)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (403)..(403)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (407)..(407)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (410)..(410)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (411)..(411)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (413)..(416)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (418)..(418)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (422)..(422)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (428)..(428)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (431)..(431)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (433)..(433)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (437)..(439)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (445)..(445)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (451)..(451)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (456)..(456)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (459)..(459)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (465)..(469)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (481)..(481)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (482)..(482)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (484)..(484)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (490)..(490)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (494)..(494)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (495)..(502)
<223>  RuvC-like domain

<220>
<221>  misc_feature
<222>  (497)..(497)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (506)..(506)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (510)..(510)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (513)..(513)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (514)..(514)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (517)..(517)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (520)..(520)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (525)..(525)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (526)..(526)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (529)..(529)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (531)..(531)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (532)..(532)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (534)..(534)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (542)..(542)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (546)..(546)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (547)..(547)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (553)..(553)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (555)..(575)
<223>  HNH-like domain

<220>
<221>  misc_feature
<222>  (556)..(556)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (560)..(560)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (563)..(563)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (565)..(565)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (567)..(567)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (579)..(579)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (582)..(582)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (583)..(583)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (585)..(585)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (588)..(588)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (590)..(590)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (592)..(592)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (594)..(596)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (610)..(610)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (616)..(616)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (628)..(628)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (631)..(631)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (633)..(633)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (634)..(634)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (636)..(636)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (638)..(641)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (643)..(645)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (653)..(653)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (657)..(657)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (659)..(659)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (660)..(660)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (665)..(665)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (666)..(666)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (668)..(668)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (669)..(669)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (670)..(677)
<223>  RuvC-like domain

<220>
<221>  misc_feature
<222>  (680)..(680)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (681)..(681)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (683)..(683)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (686)..(686)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (696)..(696)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (697)..(697)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (704)..(704)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (708)..(708)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (710)..(710)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (711)..(711)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (714)..(714)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (717)..(720)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (722)..(722)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (725)..(725)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (727)..(727)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (733)..(735)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (740)..(740)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (742)..(742)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (749)..(754)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (758)..(761)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (763)..(768)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (771)..(771)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (774)..(777)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (782)..(782)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (784)..(786)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (788)..(788)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (790)..(790)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (795)..(795)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (799)..(799)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (801)..(801)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (802)..(802)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (804)..(804)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (806)..(813)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (815)..(818)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (820)..(820)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (823)..(827)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (829)..(829)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (830)..(830)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (832)..(832)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (835)..(837)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (842)..(844)
<223>  Each Xaa can independently be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (846)..(846)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (848)..(848)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (851)..(851)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (857)..(857)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (857)..(857)
<223>  Xaa can be any naturally occurring amino acid

<400>  14

Met Lys Tyr Xaa Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly Trp 
1               5                   10                  15      


Ala Val Thr Asp Xaa Tyr Xaa Xaa Lys Xaa Lys Gly Xaa Xaa Xaa Ile 
            20                  25                  30          


Xaa Lys Asn Xaa Gly Leu Phe Asp Gly Thr Ala Arg Xaa Arg Thr Ala 
        35                  40                  45              


Arg Arg Arg Arg Arg Xaa Asn Arg Ile Tyr Leu Gln Ile Phe Xaa Glu 
    50                  55                  60                  


Met Asp Phe Phe Arg Leu Xaa Ser Phe Val Xaa Xaa Lys Xaa Xaa Xaa 
65                  70                  75                  80  


Pro Xaa Phe Xaa Xaa Glu Tyr His Xaa Xaa Pro Thr Ile Tyr His Leu 
                85                  90                  95      


Arg Xaa Leu Xaa Lys Asp Leu Arg Leu Xaa Tyr Leu Ala Leu Ala His 
            100                 105                 110         


Xaa Ile Lys Xaa Arg Gly Asn Phe Leu Ile Glu Gly Xaa Xaa Asn Xaa 
        115                 120                 125             


Xaa Xaa Xaa Xaa Xaa Tyr Xaa Phe Xaa Ile Xaa Xaa Xaa Xaa Xaa Xaa 
    130                 135                 140                 


Xaa Xaa Xaa Pro Glu Lys Gly Phe Xaa Xaa Xaa Leu Xaa Gly Xaa Phe 
145                 150                 155                 160 


Xaa Phe Xaa Leu Glu Xaa Xaa Xaa Lys Xaa Xaa Tyr Xaa Xaa Xaa Leu 
                165                 170                 175     


Xaa Leu Leu Ile Gly Asp Xaa Tyr Xaa Xaa Xaa Phe Xaa Ala Lys Xaa 
            180                 185                 190         


Xaa Xaa Xaa Leu Ser Xaa Xaa Val Thr Xaa Ala Leu Ser Xaa Xaa Met 
        195                 200                 205             


Ile Xaa Arg Xaa Xaa His Asp Leu Leu Lys Xaa Xaa Tyr Xaa Glu Xaa 
    210                 215                 220                 


Phe Xaa Lys Gly Tyr Ala Gly Tyr Ile Asp Gly Xaa Gln Phe Tyr Xaa 
225                 230                 235                 240 


Xaa Lys Leu Xaa Xaa Xaa Gly Xaa Xaa Xaa Lys Xaa Xaa Xaa Glu Xaa 
                245                 250                 255     


Xaa Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Xaa Ile Pro Xaa Gln 
            260                 265                 270         


Xaa His Leu Glu Xaa Ala Ile Xaa Xaa Gln Xaa Tyr Pro Phe Leu Asn 
        275                 280                 285             


Xaa Xaa Ile Xaa Xaa Xaa Thr Phe Arg Ile Pro Tyr Xaa Val Gly Pro 
    290                 295                 300                 


Leu Ala Gly Xaa Ser Phe Ala Trp Arg Lys Ile Pro Trp Asn Xaa Xaa 
305                 310                 315                 320 


Xaa Xaa Asp Ser Ala Phe Ile Xaa Xaa Met Thr Asp Leu Pro Xaa Xaa 
                325                 330                 335     


Xaa Val Leu Pro Lys His Ser Leu Tyr Xaa Xaa Val Tyr Asn Glu Leu 
            340                 345                 350         


Thr Lys Val Xaa Xaa Xaa Xaa Xaa Xaa Lys Xaa Ile Phe Lys Arg Lys 
        355                 360                 365             


Val Xaa Xaa Xaa Xaa Gly Xaa Xaa Phe Asn Xaa Ser Thr Tyr His Asp 
    370                 375                 380                 


Leu Xaa Xaa Xaa Xaa Xaa Leu Asp Xaa Asn Xaa Xaa Glu Xaa Ile Xaa 
385                 390                 395                 400 


Leu Thr Xaa Phe Glu Asp Xaa Met Ile Xaa Xaa Leu Xaa Xaa Xaa Xaa 
                405                 410                 415     


Lys Xaa Leu Arg Arg Xaa Tyr Thr Gly Trp Gly Xaa Leu Ser Xaa Leu 
            420                 425                 430         


Xaa Gly Ile Arg Xaa Xaa Xaa Ser Thr Ile Leu Asp Xaa Leu Asp Asn 
        435                 440                 445             


Arg Asn Xaa Met Gln Leu Ile Xaa Asp Leu Xaa Phe Lys Ile Lys Gln 
    450                 455                 460                 


Xaa Xaa Xaa Xaa Xaa Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln 
465                 470                 475                 480 


Xaa Xaa Lys Xaa Val Asp Glu Leu Val Xaa Met Gly Pro Xaa Ile Val 
                485                 490                 495     


Xaa Glu Met Ala Arg Glu Asn Gln Thr Xaa Gly Asn Ser Xaa Arg Lys 
            500                 505                 510         


Xaa Xaa Lys Glu Xaa Gly Ser Xaa Ile Leu Lys Glu Xaa Xaa Asn Leu 
        515                 520                 525             


Xaa Asn Xaa Xaa Leu Xaa Leu Tyr Tyr Leu Gln Asn Gly Xaa Asp Met 
    530                 535                 540                 


Tyr Xaa Xaa Leu Asp Ile Leu Ser Xaa Tyr Asp Xaa Asp His Ile Xaa 
545                 550                 555                 560 


Pro Gln Xaa Phe Xaa Asp Xaa Ser Ile Asp Asn Val Leu Ser Asn Arg 
                565                 570                 575     


Lys Asp Xaa Val Pro Xaa Xaa Val Xaa Lys Lys Xaa Trp Xaa Leu Xaa 
            580                 585                 590         


Leu Xaa Xaa Xaa Arg Lys Phe Asp Leu Thr Lys Ala Glu Arg Gly Gly 
        595                 600                 605             


Leu Xaa Asp Lys Ala Phe Ile Xaa Arg Gln Leu Val Glu Thr Arg Gln 
    610                 615                 620                 


Ile Thr Lys Xaa Val Ala Xaa Leu Xaa Xaa Asn Xaa Asp Xaa Xaa Xaa 
625                 630                 635                 640 


Xaa Val Xaa Xaa Xaa Thr Leu Lys Ser Leu Val Ser Xaa Phe Arg Lys 
                645                 650                 655     


Xaa Phe Xaa Xaa Leu Tyr Lys Val Xaa Xaa Asn Xaa Xaa His His Ala 
            660                 665                 670         


His Asp Ala Tyr Leu Asn Val Xaa Xaa Leu Xaa Tyr Pro Xaa Leu Glu 
        675                 680                 685             


Glu Phe Val Tyr Gly Asp Tyr Xaa Xaa Lys Ala Thr Lys Phe Tyr Xaa 
    690                 695                 700                 


Asn Ile Met Xaa Phe Xaa Xaa Gly Glu Xaa Trp Lys Xaa Xaa Xaa Xaa 
705                 710                 715                 720 


Val Xaa Met Gln Xaa Asn Xaa Val Lys Lys Glu Gln Xaa Xaa Xaa Pro 
                725                 730                 735     


Lys Asn Ser Xaa Leu Xaa Lys Asp Lys Tyr Gly Gly Xaa Xaa Xaa Xaa 
            740                 745                 750         


Xaa Xaa Lys Gly Lys Xaa Xaa Xaa Xaa Ile Xaa Xaa Xaa Xaa Xaa Xaa 
        755                 760                 765             


Phe Leu Xaa Gly Tyr Xaa Xaa Xaa Xaa Leu Pro Lys Tyr Xaa Leu Xaa 
    770                 775                 780                 


Xaa Xaa Gly Xaa Arg Xaa Leu Ala Ser Glu Xaa Lys Gly Asn Xaa Leu 
785                 790                 795                 800 


Xaa Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Glu Xaa Xaa 
                805                 810                 815     


Xaa Xaa Phe Xaa Ala Asn Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa Gly Xaa 
            820                 825                 830         


Ala Phe Xaa Xaa Xaa Ile Arg Arg Tyr Xaa Xaa Xaa Thr Xaa Ile Xaa 
        835                 840                 845             


Gln Ser Xaa Thr Gly Leu Tyr Glu Xaa Arg Leu 
    850                 855                 


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RuvC-like domain


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is Val or His

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa is Ile, Leu, or Val

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Xaa is Met or Thr

<400>  15

Ile Xaa Xaa Glu Xaa Ala Arg Glu 
1               5               


<210>  16
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RuvC-like domain


<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa is Ile, Leu, or Val

<400>  16

Ile Val Xaa Glu Met Ala Arg Glu 
1               5               


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RuvC-like domain


<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is His or Leu

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Xaa is Arg or Val

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa is Glu or Val

<400>  17

His His Ala Xaa Asp Ala Xaa Xaa 
1               5               


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RuvC-like domain

<400>  18

His His Ala His Asp Ala Tyr Leu 
1               5               


<210>  19
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal RuvC-like domain


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is Lys or Pro

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is Val, Leu, Ile, or Phe

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Xaa is Gly, Ala, or Ser

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Xaa is Leu, Ile, Val, or Phe

<220>
<221>  MOD_RES
<222>  (7)..(26)
<223>  N-terminal RuvC-like domain, each Xaa can be any amino acid or 
       absent, region may encompass 5-20 residues

<220>
<221>  VARIANT
<222>  (29)..(29)
<223>  Xaa is Asp, Glu, Asn, or Gln

<400>  19

Lys Xaa Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Thr Asp Xaa Tyr 
            20                  25                  30  


<210>  20
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal RuvC-like domain


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is Ile, Val, Met, Leu, or Thr

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is Thr, Ile, Val, Ser, Asn, Tyr, Glu, or Leu

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Xaa is Asn, Ser, Gly, Ala, Asp, Thr, Arg, Met, or Phe

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Xaa is Ser, Tyr, Asn, or Phe

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Xaa is Val, Ile, Leu, Cys, Thr, or Phe

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa is Trp, Phe, Val, Tyr, Ser, or Leu

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  Xaa is Ala, Ser, Cys, Val, or Gly

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa is Val, Ile, Leu, Ala, Met, or His

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Any amino acid or absent

<400>  20

Asp Xaa Gly Xaa Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa 
1               5                   10          


<210>  21
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal RuvC-like domain


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is Ile, Val, Met, Leu, or Thr

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is Thr, Ile, Val, Ser, Asn, Tyr, Glu, or Leu

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Xaa is Asn, Ser, Gly, Ala, Asp, Thr, Arg, Met, or Phe

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Xaa is Val, Ile, Leu, Cys, Thr, or Phe

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa is Trp, Phe, Val, Tyr, Ser, or Leu

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  Xaa is Ala, Ser, Cys, Val, or Gly

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa is Val, Ile, Leu, Ala, Met, or His

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Any amino acid or absent

<400>  21

Asp Xaa Gly Xaa Xaa Ser Xaa Gly Xaa Xaa Xaa Xaa 
1               5                   10          


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal RuvC-like domain


<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa is Thr, Ile, Val, Ser, Asn, Tyr, Glu, or Leu

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Xaa is Asn, Ser, Gly, Ala, Asp, Thr, Arg, Met, or Phe

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa is Val, Ile, Leu, Ala, Met, or His

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Any amino acid or absent

<400>  22

Asp Ile Gly Xaa Xaa Ser Val Gly Trp Ala Xaa Xaa 
1               5                   10          


<210>  23
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal RuvC-like domain


<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Any non-polar alkyl amino acid or a hydroxyl amino acid

<400>  23

Asp Ile Gly Thr Asn Ser Val Gly Trp Ala Val Xaa 
1               5                   10          


<210>  24
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HNH-like domain


<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa is Lys or Arg

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Xaa is Val or Thr

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  Xaa is Gly or Asp

<220>
<221>  VARIANT
<222>  (14)..(14)
<223>  Xaa is Glu, Gln, or Asp

<220>
<221>  VARIANT
<222>  (15)..(15)
<223>  Xaa is Glu or Asp

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  Xaa is Asp, Asn, or His

<220>
<221>  VARIANT
<222>  (20)..(20)
<223>  Xaa is Tyr, Arg, or Asn

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Xaa is Gln, Asp, or Asn

<220>
<221>  MOD_RES
<222>  (25)..(64)
<223>  HNH-like domain, each Xaa can be any amino acid or absent, region
       may encompass 15-40 residues

<220>
<221>  VARIANT
<222>  (67)..(67)
<223>  Xaa is Gly or Glu

<220>
<221>  VARIANT
<222>  (69)..(69)
<223>  Xaa is Ser or Gly

<220>
<221>  VARIANT
<222>  (71)..(71)
<223>  Xaa is Asp or Asn

<400>  24

Leu Tyr Tyr Leu Gln Asn Gly Xaa Asp Met Tyr Xaa Xaa Xaa Xaa Leu 
1               5                   10                  15      


Asp Ile Xaa Xaa Leu Ser Xaa Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Asn Arg Xaa Lys Xaa Asp Xaa Val Pro 
65                  70              


<210>  25
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HNH-like domain


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa is Asp, Glu, Gln, or Asn

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is Leu, Ile, Arg, Gln, Val, Met, or Lys

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa is Asp or Glu

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Xaa is Ile, Val, Thr, Ala, or Leu

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Xaa is Val, Tyr, Ile, Leu, Phe, or Trp

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa is Gln, His, Arg, Lys, Tyr, Ile, Leu, Phe, or Trp

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa is Ser, Ala, Asp, Thr, or Lys

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  Xaa is Phe, Leu, Val, Lys, Tyr, Met, Ile, Arg, Ala, Glu, Asp, or 
       Gln

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa is Leu, Arg, Thr, Ile, Val, Ser, Cys, Tyr, Lys, Phe, or Gly

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Xaa is Lys, Gln, Tyr, Thr, Phe, Leu, Trp, Met, Ala, Glu, Gly, or 
       Ser

<220>
<221>  VARIANT
<222>  (13)..(13)
<223>  Xaa is Asp, Ser, Asn, Arg, Leu, or Thr

<220>
<221>  VARIANT
<222>  (14)..(14)
<223>  Xaa is Asp, Asn, or Ser

<220>
<221>  VARIANT
<222>  (15)..(15)
<223>  Xaa is Ser, Ala, Thr, Gly, or Arg

<220>
<221>  VARIANT
<222>  (16)..(16)
<223>  Xaa is Ile, Leu, Phe, Ser, Arg, Tyr, Gln, Trp, Asp, Lys, or His

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Xaa is Asp, Ser, Ile, Asn, Glu, Ala, His, Phe, Leu, Gln, Met, 
       Gly, Tyr, or Val

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  Xaa is Lys, Leu, Arg, Met, Thr, or Phe

<220>
<221>  VARIANT
<222>  (20)..(20)
<223>  Xaa is Val, Leu, Ile, Ala, or Thr

<220>
<221>  VARIANT
<222>  (21)..(21)
<223>  Xaa is Leu, Ile, Val, or Ala

<220>
<221>  VARIANT
<222>  (22)..(22)
<223>  Xaa is Thr, Val, Cys, Glu, Ser, or Ala

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Xaa is Arg, Phe, Thr, Trp, Glu, Leu, Asn, Cys, Lys, Val, Ser, 
       Gln, Ile, Tyr, His, or Ala

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Xaa is Ser, Pro, Arg, Lys, Asn, Ala, His, Gln, Gly, or Leu

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  Xaa is Asp, Gly, Thr, Asn, Ser, Lys, Ala, Ile, Glu, Leu, Gln, 
       Arg, or Tyr

<220>
<221>  VARIANT
<222>  (26)..(26)
<223>  Xaa is Lys, Val, Ala, Glu, Tyr, Ile, Cys, Leu, Ser, Thr, Gly, 
       Lys, Met, Asp, or Phe

<400>  25

Xaa Xaa Xaa His Xaa Xaa Pro Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asn 
            20                  25          


<210>  26
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HNH-like domain


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa is Asp or Glu

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is Leu, Ile, Arg, Gln, Val, Met, or Lys

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa is Asp or Glu

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  Xaa is Ile, Val, Thr, Ala, or Leu

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Xaa is Val, Tyr, Ile, Leu, Phe, or Trp

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa is Gln, His, Arg, Lys, Tyr, Ile, Leu, Phe, or Trp

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  Xaa is Phe, Leu, Val, Lys, Tyr, Met, Ile, Arg, Ala, Glu, Asp, or 
       Gln

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa is Leu, Arg, Thr, Ile, Val, Ser, Cys, Tyr, Lys, Phe, or Gly

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Xaa is Lys, Gln, Tyr, Thr, Phe, Leu, Trp, Met, Ala, Glu, Gly, or 
       Ser

<220>
<221>  VARIANT
<222>  (16)..(16)
<223>  Xaa is Ile, Leu, Phe, Ser, Arg, Tyr, Gln, Trp, Asp, Lys, or His

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Xaa is Asp, Ser, Ile, Asn, Glu, Ala, His, Phe, Leu, Gln, Met, 
       Gly, Tyr, or Val

<220>
<221>  VARIANT
<222>  (22)..(22)
<223>  Xaa is Thr, Val, Cys, Glu, Ser, or Ala

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Xaa is Arg, Phe, Thr, Trp, Glu, Leu, Asn, Cys, Lys, Val, Ser, 
       Gln, Ile, Tyr, His, or Ala

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Xaa is Ser, Pro, Arg, Lys, Asn, Ala, His, Gln, Gly, or Leu

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  Xaa is Asp, Gly, Thr, Asn, Ser, Lys, Ala, Ile, Glu, Leu, Gln, 
       Arg, or Tyr

<220>
<221>  VARIANT
<222>  (26)..(26)
<223>  Xaa is Lys, Val, Ala, Glu, Tyr, Ile, Cys, Leu, Ser, Thr, Gly, 
       Lys, Met, Asp, or Phe

<400>  26

Xaa Xaa Xaa His Xaa Xaa Pro Xaa Ser Xaa Xaa Xaa Asp Asp Ser Xaa 
1               5                   10                  15      


Xaa Asn Lys Val Leu Xaa Xaa Xaa Xaa Xaa Asn 
            20                  25          


<210>  27
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HNH-like domain


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  Xaa is Asp or Glu

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Xaa is Asp or Glu

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Xaa is Gln, His, Arg, Lys, Tyr, Ile, Leu, or Trp

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  Xaa is Phe, Leu, Val, Lys, Tyr, Met, Ile, Arg, Ala, Glu, Asp, or 
       Gln

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa is Leu, Arg, Thr, Ile, Val, Ser, Cys, Tyr, Lys, Phe, or Gly

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Xaa is Lys, Gln, Tyr, Thr, Phe, Leu, Trp, Met, Ala, Glu, Gly, or 
       Ser

<220>
<221>  VARIANT
<222>  (16)..(16)
<223>  Xaa is Ile, Leu, Phe, Ser, Arg, Tyr, Gln, Trp, Asp, Lys, or His

<220>
<221>  VARIANT
<222>  (17)..(17)
<223>  Xaa is Asp, Ser, Ile, Asn, Glu, Ala, His, Phe, Leu, Gln, Met, 
       Gly, Tyr, or Val

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Xaa is Arg, Phe, Thr, Trp, Glu, Leu, Asn, Cys, Lys, Val, Ser, 
       Gln, Ile, Tyr, His, or Ala

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Xaa is Ser, Pro, Arg, Lys, Asn, Ala, His, Gln, Gly, or Leu

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  Xaa is Asp, Gly, Thr, Asn, Ser, Lys, Ala, Ile, Glu, Leu, Gln, 
       Arg, or Tyr

<220>
<221>  VARIANT
<222>  (26)..(26)
<223>  Xaa is Lys, Val, Ala, Glu, Tyr, Ile, Cys, Leu, Ser, Thr, Gly, 
       Lys, Met, Asp, or Phe

<400>  27

Xaa Val Xaa His Ile Val Pro Xaa Ser Xaa Xaa Xaa Asp Asp Ser Xaa 
1               5                   10                  15      


Xaa Asn Lys Val Leu Thr Xaa Xaa Xaa Xaa Asn 
            20                  25          


<210>  28
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HNH-like domain


<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  Xaa is Ile or Val

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  Xaa is Ile or Val

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Xaa is Ala or Ser

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa is Ile or Leu

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Xaa is Lys or Thr

<220>
<221>  VARIANT
<222>  (14)..(14)
<223>  Xaa is Asp or Asn

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  Xaa is Arg, Lys, or Leu

<220>
<221>  VARIANT
<222>  (22)..(22)
<223>  Xaa is Thr or Val

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Xaa is Ser or Arg

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  Xaa is Lys, Asp, or Ala

<220>
<221>  VARIANT
<222>  (26)..(26)
<223>  Xaa is Glu, Lys, Gly, or Asn

<400>  28

Asp Xaa Asp His Ile Xaa Pro Gln Xaa Phe Xaa Xaa Asp Xaa Ser Ile 
1               5                   10                  15      


Asp Asn Xaa Val Leu Xaa Xaa Ser Xaa Xaa Asn 
            20                  25          


<210>  29
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  targeting region

<220>
<221>  misc_feature
<222>  (21)..(42)
<223>  first complementarity domain

<220>
<221>  misc_feature
<222>  (43)..(46)
<223>  linking domain

<220>
<221>  misc_feature
<222>  (47)..(70)
<223>  second complementarity domain

<400>  29
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuguuu uggaaacaaa acagcauagc       60

aaguuaaaau aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc          116


<210>  30
<211>  116
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  targeting region

<220>
<221>  misc_feature
<222>  (21)..(42)
<223>  first complementarity domain

<220>
<221>  misc_feature
<222>  (43)..(46)
<223>  linking domain

<220>
<221>  misc_feature
<222>  (47)..(70)
<223>  second complementarity domain

<400>  30
nnnnnnnnnn nnnnnnnnnn guauuagagc uaugcuguau uggaaacaau acagcauagc       60

aaguuaauau aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc          116


<210>  31
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  targeting domain

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  first complementarity domain

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  linking domain

<220>
<221>  misc_feature
<222>  (37)..(50)
<223>  second complementarity domain

<220>
<221>  misc_feature
<222>  (51)..(62)
<223>  proximal domain

<400>  31
nnnnnnnnnn nnnnnnnnnn guuuaagagc uagaaauagc aaguuuaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  32
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA proximal and tail domains derived from S. pyogenes

<400>  32
aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcu                     47


<210>  33
<211>  49
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA proximal and tail domains

<400>  33
aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cgguggugc                   49


<210>  34
<211>  51
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA proximal and tail domains

<400>  34
aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugcggau c                51


<210>  35
<211>  31
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA proximal and tail domains

<400>  35
aaggcuaguc cguuaucaac uugaaaaagu g                                      31


<210>  36
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA proximal and tail domains

<400>  36
aaggcuaguc cguuauca                                                     18


<210>  37
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA proximal and tail domains

<400>  37
aaggcuaguc cg                                                           12


<210>  38
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Unimolecular gRNA derived from S. aureus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<400>  38
nnnnnnnnnn nnnnnnnnnn guuuuaguac ucuggaaaca gaaucuacua aaacaaggca       60

aaaugccgug uuuaucucgu caacuuguug gcgagauuuu uu                         102


<210>  39
<211>  42
<212>  RNA
<213>  Artificial

<220>
<223>  Modular gRNA derived from S. pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (21)..(42)
<223>  First complementarity domain

<400>  39
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuguuu ug                          42


<210>  40
<211>  85
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Modular gRNA derived from S. pyogenes


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  5' extension domain

<220>
<221>  misc_feature
<222>  (10)..(33)
<223>  Second complementarity domain

<220>
<221>  misc_feature
<222>  (34)..(45)
<223>  Proximal domain

<220>
<221>  misc_feature
<222>  (46)..(85)
<223>  Tail domain

<400>  40
ggaaccauuc aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa       60

aguggcaccg agucggugcu uuuuu                                             85


<210>  41
<211>  62
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Unimolecular gRNA derived from S. pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  First complementarity domain

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  Linking domain

<220>
<221>  misc_feature
<222>  (37)..(50)
<223>  Second complementarity domain

<220>
<221>  misc_feature
<222>  (51)..(62)
<223>  Proximal domain

<400>  41
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cg                                                                      62


<210>  42
<211>  102
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Unimolecular gRNA derived from S. pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  First complementarity domain

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  Linking domain

<220>
<221>  misc_feature
<222>  (37)..(50)
<223>  Second complementarity domain

<220>
<221>  misc_feature
<222>  (51)..(62)
<223>  Proximal domain

<220>
<221>  misc_feature
<222>  (63)..(102)
<223>  Tail domain

<400>  42
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu uu                         102


<210>  43
<211>  75
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Unimolecular gRNA derived from S. pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (21)..(36)
<223>  First complementarity domain

<220>
<221>  misc_feature
<222>  (37)..(40)
<223>  Linking domain

<220>
<221>  misc_feature
<222>  (41)..(58)
<223>  Second complementarity domain

<220>
<221>  misc_feature
<222>  (59)..(70)
<223>  Proximal domain

<220>
<221>  misc_feature
<222>  (71)..(75)
<223>  Tail domain

<400>  43
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcugaaa agcauagcaa guuaaaauaa       60

ggcuaguccg uuauc                                                        75


<210>  44
<211>  87
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Unimolecular gRNA derived from S. pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  First complementarity domain

<220>
<221>  misc_feature
<222>  (43)..(46)
<223>  Linking domain

<220>
<221>  misc_feature
<222>  (57)..(70)
<223>  Second complementarity domain

<220>
<221>  misc_feature
<222>  (71)..(82)
<223>  Proximal domain

<220>
<221>  misc_feature
<222>  (83)..(87)
<223>  Tail domain

<400>  44
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuguuu uggaaacaaa acagcauagc       60

aaguuaaaau aaggcuaguc cguuauc                                           87


<210>  45
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Modular gRNA derived from S. thermophilus


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  a, c, u, g, unknown or other

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (21)..(42)
<223>  First complementarity domain

<400>  45
nnnnnnnnnn nnnnnnnnnn guuuuagagc uguguuguuu cg                          42


<210>  46
<211>  78
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Modular gRNA derived from S. thermophilus


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  5' extension domain

<220>
<221>  misc_feature
<222>  (4)..(27)
<223>  Second complementarity domain

<220>
<221>  misc_feature
<222>  (28)..(40)
<223>  Proximal domain

<220>
<221>  misc_feature
<222>  (41)..(78)
<223>  Tail domain

<400>  46
gggcgaaaca acacagcgag uuaaaauaag gcuuaguccg uacucaacuu gaaaaggugg       60

caccgauucg guguuuuu                                                     78


<210>  47
<211>  85
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Modular gRNA derived from S. pyogenes

<400>  47
gaaccauuca aaacagcaua gcaaguuaaa auaaggcuag uccguuauca acuugaaaaa       60

guggcaccga gucggugcuu uuuuu                                             85


<210>  48
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA from S. pyogenes


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<400>  48
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  49
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  First complementarity domain

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  Linking domain

<220>
<221>  misc_feature
<222>  (37)..(50)
<223>  Second complementarity domain

<220>
<221>  misc_feature
<222>  (51)..(62)
<223>  Proximal domain

<400>  49
nnnnnnnnnn nnnnnnnnnn guauuagagc uagaaauagc aaguuaauau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  50
<211>  104
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (21)..(36)
<223>  First complementarity domain

<220>
<221>  misc_feature
<222>  (37)..(40)
<223>  Linking domain

<220>
<221>  misc_feature
<222>  (41)..(58)
<223>  Second complementarity domain

<400>  50
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcugaaa agcauagcaa guuaaaauaa       60

ggcuaguccg uuaucaacuu gaaaaagugg caccgagucg gugc                       104


<210>  51
<211>  106
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n is a, c, g, or u

<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Targeting domain

<220>
<221>  misc_feature
<222>  (21)..(37)
<223>  First complementarity domain

<220>
<221>  misc_feature
<222>  (38)..(41)
<223>  Linking domain

<220>
<221>  misc_feature
<222>  (42)..(60)
<223>  Second complementarity domain

<400>  51
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuggaa acagcauagc aaguuaaaau       60

aaggcuaguc cguuaucaac uugaaaaagu ggcaccgagu cggugc                     106


<210>  52
<211>  12
<212>  PRT
<213>  Peptoniphilus duerdenii

<400>  52

Asp Ile Gly Thr Ala Ser Val Gly Trp Ala Val Thr 
1               5                   10          


<210>  53
<211>  12
<212>  PRT
<213>  Treponema denticola

<400>  53

Asp Val Gly Thr Gly Ser Val Gly Trp Ala Val Thr 
1               5                   10          


<210>  54
<211>  12
<212>  PRT
<213>  S. mutans

<400>  54

Asp Ile Gly Thr Asn Ser Val Gly Trp Ala Val Val 
1               5                   10          


<210>  55
<211>  12
<212>  PRT
<213>  S. pyogenes

<400>  55

Asp Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile 
1               5                   10          


<210>  56
<211>  12
<212>  PRT
<213>  L. innocua

<400>  56

Asp Ile Gly Thr Asn Ser Val Gly Trp Ala Val Leu 
1               5                   10          


<210>  57
<211>  12
<212>  PRT
<213>  Flavobacterium branchiophilum FL-15

<400>  57

Asp Leu Gly Thr Asn Ser Ile Gly Trp Ala Val Val 
1               5                   10          


<210>  58
<211>  11
<212>  PRT
<213>  Pedobacter glucosidilyticus

<400>  58

Asp Leu Gly Thr Asn Ser Ile Gly Trp Ala Ile 
1               5                   10      


<210>  59
<211>  12
<212>  PRT
<213>  Bacteroides fragilis, NCTC 9343

<400>  59

Asp Leu Gly Thr Asn Ser Ile Gly Trp Ala Leu Val 
1               5                   10          


<210>  60
<211>  12
<212>  PRT
<213>  Fusobacterium nucleatum

<400>  60

Asp Ile Gly Thr Asn Ser Val Gly Trp Cys Val Thr 
1               5                   10          


<210>  61
<211>  12
<212>  PRT
<213>  Acidaminococcus sp. D21

<400>  61

Asp Ile Gly Thr Asn Ser Val Gly Tyr Ala Val Thr 
1               5                   10          


<210>  62
<211>  12
<212>  PRT
<213>  Coprococcus catus GD-7

<400>  62

Asp Met Gly Thr Gly Ser Leu Gly Trp Ala Val Thr 
1               5                   10          


<210>  63
<211>  12
<212>  PRT
<213>  Oenococcus kitaharae DSM 17330

<400>  63

Asp Ile Gly Thr Ser Ser Val Gly Trp Ala Ala Ile 
1               5                   10          


<210>  64
<211>  12
<212>  PRT
<213>  Catenibacterium mitsuokai DSM 15897

<400>  64

Asp Leu Gly Thr Gly Ser Val Gly Trp Ala Val Val 
1               5                   10          


<210>  65
<211>  12
<212>  PRT
<213>  Mycoplasma gallisepticum str. F

<400>  65

Asp Leu Gly Val Gly Ser Val Gly Trp Ala Ile Val 
1               5                   10          


<210>  66
<211>  12
<212>  PRT
<213>  Mycoplasma ovipneumoniae SC01

<400>  66

Asp Leu Gly Ile Ala Ser Ile Gly Trp Ala Ile Ile 
1               5                   10          


<210>  67
<211>  12
<212>  PRT
<213>  Mycoplasma canis PG 14

<400>  67

Asp Leu Gly Ile Ala Ser Val Gly Trp Ala Ile Val 
1               5                   10          


<210>  68
<211>  12
<212>  PRT
<213>  Mycoplasma synoviae 53

<400>  68

Asp Leu Gly Val Ala Ser Val Gly Trp Ser Ile Val 
1               5                   10          


<210>  69
<211>  12
<212>  PRT
<213>  Eubacterium rectale

<400>  69

Asp Ile Gly Ile Ala Ser Val Gly Trp Ala Ile Leu 
1               5                   10          


<210>  70
<211>  12
<212>  PRT
<213>  Enterococcus faecalis TX0012

<400>  70

Asp Leu Gly Ile Ser Ser Val Gly Trp Ser Val Ile 
1               5                   10          


<210>  71
<211>  12
<212>  PRT
<213>  Ilyobacter polytropus DSM 2926

<400>  71

Asp Ile Gly Ile Ala Ser Val Gly Trp Ser Val Ile 
1               5                   10          


<210>  72
<211>  12
<212>  PRT
<213>  Ruminococcus albus 8

<400>  72

Asp Val Gly Ile Gly Ser Ile Gly Trp Ala Val Ile 
1               5                   10          


<210>  73
<211>  12
<212>  PRT
<213>  Elusimicrobium minutum Pei191

<400>  73

Asp Leu Gly Val Gly Ser Ile Gly Phe Ala Ile Val 
1               5                   10          


<210>  74
<211>  12
<212>  PRT
<213>  Akkermansia muciniphila

<400>  74

Asp Ile Gly Tyr Ala Ser Ile Gly Trp Ala Val Ile 
1               5                   10          


<210>  75
<211>  12
<212>  PRT
<213>  Prevotella ruminicola

<400>  75

Asp Thr Gly Thr Asn Ser Leu Gly Trp Ala Ile Val 
1               5                   10          


<210>  76
<211>  12
<212>  PRT
<213>  Cand. Puniceispirillum marinum

<400>  76

Asp Leu Gly Thr Asn Ser Ile Gly Trp Cys Leu Leu 
1               5                   10          


<210>  77
<211>  12
<212>  PRT
<213>  Rhodospirillum rubrum

<400>  77

Asp Ile Gly Thr Asp Ser Leu Gly Trp Ala Val Phe 
1               5                   10          


<210>  78
<211>  12
<212>  PRT
<213>  Lactobacillus rhamnosus GG

<400>  78

Asp Ile Gly Ser Asn Ser Ile Gly Phe Ala Val Val 
1               5                   10          


<210>  79
<211>  12
<212>  PRT
<213>  Sphaerochaeta globus str. Buddy

<400>  79

Asp Leu Gly Val Gly Ser Ile Gly Val Ala Val Ala 
1               5                   10          


<210>  80
<211>  12
<212>  PRT
<213>  Rhodopseudomonas palustris

<400>  80

Asp Leu Gly Ile Ala Ser Cys Gly Trp Gly Val Val 
1               5                   10          


<210>  81
<211>  12
<212>  PRT
<213>  Mycoplasma mobile 163K

<400>  81

Asp Leu Gly Ile Ala Ser Val Gly Trp Cys Leu Thr 
1               5                   10          


<210>  82
<211>  12
<212>  PRT
<213>  Streptococcus thermophilus LMD-9

<400>  82

Asp Ile Gly Ile Gly Ser Val Gly Val Gly Ile Leu 
1               5                   10          


<210>  83
<211>  12
<212>  PRT
<213>  Staphylococcus lugdunensis M23590

<400>  83

Asp Ile Gly Ile Thr Ser Val Gly Tyr Gly Leu Ile 
1               5                   10          


<210>  84
<211>  12
<212>  PRT
<213>  Eubacterium dolichum DSM 3991

<400>  84

Asp Ile Gly Ile Thr Ser Val Gly Phe Gly Ile Ile 
1               5                   10          


<210>  85
<211>  12
<212>  PRT
<213>  Lactobacillus coryniformis KCTC 3535

<400>  85

Asp Val Gly Ile Thr Ser Thr Gly Tyr Ala Val Leu 
1               5                   10          


<210>  86
<211>  12
<212>  PRT
<213>  Nitratifractor salsuginis DSM 16511

<400>  86

Asp Leu Gly Ile Thr Ser Phe Gly Tyr Ala Ile Leu 
1               5                   10          


<210>  87
<211>  12
<212>  PRT
<213>  Bifidobacterium bifidum S17

<400>  87

Asp Ile Gly Asn Ala Ser Val Gly Trp Ser Ala Phe 
1               5                   10          


<210>  88
<211>  12
<212>  PRT
<213>  Lactobacillus gasseri

<400>  88

Asp Val Gly Thr Asn Ser Cys Gly Trp Val Ala Met 
1               5                   10          


<210>  89
<211>  12
<212>  PRT
<213>  Acidothermus cellulolyticus 11B

<400>  89

Asp Val Gly Glu Arg Ser Ile Gly Leu Ala Ala Val 
1               5                   10          


<210>  90
<211>  12
<212>  PRT
<213>  Bifidobacterium longum DJO10A

<400>  90

Asp Val Gly Leu Asn Ser Val Gly Leu Ala Ala Val 
1               5                   10          


<210>  91
<211>  12
<212>  PRT
<213>  Bifidobacterium dentium

<400>  91

Asp Val Gly Leu Met Ser Val Gly Leu Ala Ala Ile 
1               5                   10          


<210>  92
<211>  12
<212>  PRT
<213>  Corynebacterium diphtheriae

<400>  92

Asp Val Gly Thr Phe Ser Val Gly Leu Ala Ala Ile 
1               5                   10          


<210>  93
<211>  12
<212>  PRT
<213>  Staphylococcus pseudintermedius ED99

<400>  93

Asp Ile Gly Thr Gly Ser Val Gly Tyr Ala Cys Met 
1               5                   10          


<210>  94
<211>  12
<212>  PRT
<213>  Capnocytophaga ochracea

<400>  94

Asp Leu Gly Thr Thr Ser Ile Gly Phe Ala His Ile 
1               5                   10          


<210>  95
<211>  12
<212>  PRT
<213>  Prevotella denticola

<400>  95

Asp Leu Gly Thr Asn Ser Ile Gly Ser Ser Val Arg 
1               5                   10          


<210>  96
<211>  12
<212>  PRT
<213>  Ralstonia solanacearum

<400>  96

Asp Ile Gly Thr Asn Ser Ile Gly Trp Ala Val Ile 
1               5                   10          


<210>  97
<211>  12
<212>  PRT
<213>  Pasteurella multocida str. Pm70

<400>  97

Asp Leu Gly Ile Ala Ser Val Gly Trp Ala Val Val 
1               5                   10          


<210>  98
<211>  12
<212>  PRT
<213>  Comamonas granuli

<400>  98

Asp Ile Gly Ile Ala Ser Val Gly Trp Ala Val Leu 
1               5                   10          


<210>  99
<211>  12
<212>  PRT
<213>  Helicobacter mustelae 12198

<400>  99

Asp Ile Gly Ile Ala Ser Ile Gly Trp Ala Val Ile 
1               5                   10          


<210>  100
<211>  12
<212>  PRT
<213>  Agathobacter rectalis

<400>  100

Asp Ile Gly Ile Ala Ser Val Gly Trp Ala Ile Ile 
1               5                   10          


<210>  101
<211>  12
<212>  PRT
<213>  Clostridium cellulolyticum H10

<400>  101

Asp Val Gly Ile Ala Ser Val Gly Trp Ala Val Ile 
1               5                   10          


<210>  102
<211>  11
<212>  PRT
<213>  Methylophilus sp. OH31

<400>  102

Asp Ile Gly Ile Ala Ser Val Gly Trp Ala Leu 
1               5                   10      


<210>  103
<211>  12
<212>  PRT
<213>  Neisseria meningitidis

<400>  103

Asp Ile Gly Ile Ala Ser Val Gly Trp Ala Met Val 
1               5                   10          


<210>  104
<211>  12
<212>  PRT
<213>  Clostridium perfringens

<400>  104

Asp Ile Gly Ile Thr Ser Val Gly Trp Ala Val Ile 
1               5                   10          


<210>  105
<211>  12
<212>  PRT
<213>  Wolinella succinogenes DSM 1740

<400>  105

Asp Leu Gly Ile Ser Ser Leu Gly Trp Ala Ile Val 
1               5                   10          


<210>  106
<211>  12
<212>  PRT
<213>  Azospirillum sp. B510

<400>  106

Asp Leu Gly Thr Asn Ser Ile Gly Trp Gly Leu Leu 
1               5                   10          


<210>  107
<211>  12
<212>  PRT
<213>  Verminephrobacter eiseniae

<400>  107

Asp Leu Gly Ser Thr Ser Leu Gly Trp Ala Ile Phe 
1               5                   10          


<210>  108
<211>  12
<212>  PRT
<213>  Campylobacter jejuni, NCTC 11168

<400>  108

Asp Ile Gly Ile Ser Ser Ile Gly Trp Ala Phe Ser 
1               5                   10          


<210>  109
<211>  12
<212>  PRT
<213>  Parvibaculum lavamentivorans DS-1

<400>  109

Asp Ile Gly Thr Thr Ser Ile Gly Phe Ser Val Ile 
1               5                   10          


<210>  110
<211>  12
<212>  PRT
<213>  Dinoroseobacter shibae DFL 12

<400>  110

Asp Ile Gly Thr Ser Ser Ile Gly Trp Trp Leu Tyr 
1               5                   10          


<210>  111
<211>  12
<212>  PRT
<213>  Nitrobacter hamburgensis X14

<400>  111

Asp Leu Gly Ser Asn Ser Leu Gly Trp Phe Val Thr 
1               5                   10          


<210>  112
<211>  12
<212>  PRT
<213>  Bradyrhizobium sp. BTAi1

<400>  112

Asp Leu Gly Ala Asn Ser Leu Gly Trp Phe Val Val 
1               5                   10          


<210>  113
<211>  15
<212>  PRT
<213>  Bacillus cereus

<400>  113

Asp Ile Gly Leu Arg Ile Gly Ile Thr Ser Cys Gly Trp Ser Ile 
1               5                   10                  15  


<210>  114
<211>  12
<212>  PRT
<213>  Sutterella wadsworthensis

<400>  114

Asp Met Gly Ala Lys Tyr Thr Gly Val Phe Tyr Ala 
1               5                   10          


<210>  115
<211>  12
<212>  PRT
<213>  Wolinella succinogenes DSM 1740

<400>  115

Asp Leu Gly Gly Lys Asn Thr Gly Phe Phe Ser Phe 
1               5                   10          


<210>  116
<211>  12
<212>  PRT
<213>  Francisella tularensis

<400>  116

Asp Leu Gly Val Lys Asn Thr Gly Val Phe Ser Ala 
1               5                   10          


<210>  117
<211>  12
<212>  PRT
<213>  Gamma proteobacterium HTCC5015

<400>  117

Asp Leu Gly Ala Lys Phe Thr Gly Val Ala Leu Tyr 
1               5                   10          


<210>  118
<211>  12
<212>  PRT
<213>  Legionella pneumophila str. Paris

<400>  118

Asp Leu Gly Gly Lys Phe Thr Gly Val Cys Leu Ser 
1               5                   10          


<210>  119
<211>  12
<212>  PRT
<213>  Parasutterella excrementihominis

<400>  119

Asp Leu Gly Gly Thr Tyr Thr Gly Thr Phe Ile Thr 
1               5                   10          


<210>  120
<211>  12
<212>  PRT
<213>  S. thermophilus

<400>  120

Asp Ile Gly Thr Asn Ser Val Gly Trp Ala Val Thr 
1               5                   10          


<210>  121
<211>  12
<212>  PRT
<213>  Eubacterium yurii

<400>  121

Asp Val Gly Thr Asn Ser Val Gly Trp Ala Val Thr 
1               5                   10          


<210>  122
<211>  12
<212>  PRT
<213>  Butyrivibrio hungatei

<400>  122

Asp Met Gly Thr Asn Ser Val Gly Trp Ala Val Thr 
1               5                   10          


<210>  123
<211>  12
<212>  PRT
<213>  Solobacterium moorei F0204

<400>  123

Asp Val Gly Thr Ser Ser Val Gly Trp Ala Val Thr 
1               5                   10          


<210>  124
<211>  27
<212>  PRT
<213>  Treponema denticola

<400>  124

Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile 
1               5                   10                  15      


Ser Asn Arg Val Leu Val Cys Ser Ser Cys Asn 
            20                  25          


<210>  125
<211>  27
<212>  PRT
<213>  Coprococcus catus GD-7

<400>  125

Asp Ile Asp His Ile Tyr Pro Gln Ser Lys Thr Met Asp Asp Ser Leu 
1               5                   10                  15      


Asn Asn Arg Val Leu Val Lys Lys Asn Tyr Asn 
            20                  25          


<210>  126
<211>  27
<212>  PRT
<213>  Peptoniphilus duerdenii

<400>  126

Asp Gln Asp His Ile Tyr Pro Lys Ser Lys Ile Tyr Asp Asp Ser Leu 
1               5                   10                  15      


Glu Asn Arg Val Leu Val Lys Lys Asn Leu Asn 
            20                  25          


<210>  127
<211>  27
<212>  PRT
<213>  Catenibacterium mitsuokai DSM 15897

<400>  127

Gln Ile Asp His Ile Val Pro Gln Ser Leu Val Lys Asp Asp Ser Phe 
1               5                   10                  15      


Asp Asn Arg Val Leu Val Val Pro Ser Glu Asn 
            20                  25          


<210>  128
<211>  27
<212>  PRT
<213>  S. mutans

<400>  128

Asp Ile Asp His Ile Ile Pro Gln Ala Phe Ile Lys Asp Asn Ser Ile 
1               5                   10                  15      


Asp Asn Arg Val Leu Thr Ser Ser Lys Glu Asn 
            20                  25          


<210>  129
<211>  27
<212>  PRT
<213>  S. thermophilus

<400>  129

Asp Ile Asp His Ile Ile Pro Gln Ala Phe Leu Lys Asp Asn Ser Ile 
1               5                   10                  15      


Asp Asn Lys Val Leu Val Ser Ser Ala Ser Asn 
            20                  25          


<210>  130
<211>  27
<212>  PRT
<213>  Oenococcus kitaharae DSM 17330

<400>  130

Asp Ile Asp His Ile Ile Pro Gln Ala Tyr Thr Lys Asp Asn Ser Leu 
1               5                   10                  15      


Asp Asn Arg Val Leu Val Ser Asn Ile Thr Asn 
            20                  25          


<210>  131
<211>  27
<212>  PRT
<213>  L. inocua

<400>  131

Asp Ile Asp His Ile Val Pro Gln Ser Phe Ile Thr Asp Asn Ser Ile 
1               5                   10                  15      


Asp Asn Leu Val Leu Thr Ser Ser Ala Gly Asn 
            20                  25          


<210>  132
<211>  27
<212>  PRT
<213>  S. pyogenes

<400>  132

Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile 
1               5                   10                  15      


Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn 
            20                  25          


<210>  133
<211>  27
<212>  PRT
<213>  Acidaminococcus sp. D21

<400>  133

Asn Ile Asp His Ile Tyr Pro Gln Ser Met Val Lys Asp Asp Ser Leu 
1               5                   10                  15      


Asp Asn Lys Val Leu Val Gln Ser Glu Ile Asn 
            20                  25          


<210>  134
<211>  27
<212>  PRT
<213>  Lactobacillus rhamnosus GG

<400>  134

Asp Ile Asp His Ile Leu Pro Gln Ser Leu Ile Lys Asp Asp Ser Leu 
1               5                   10                  15      


Asp Asn Arg Val Leu Val Asn Ala Thr Ile Asn 
            20                  25          


<210>  135
<211>  27
<212>  PRT
<213>  Lactobacillus gasseri

<400>  135

Asp Ile Asp His Ile Leu Pro Gln Ser Phe Ile Lys Asp Asp Ser Leu 
1               5                   10                  15      


Glu Asn Arg Val Leu Val Lys Lys Ala Val Asn 
            20                  25          


<210>  136
<211>  27
<212>  PRT
<213>  Staphylococcus pseudintermedius ED99

<400>  136

Glu Val Asp His Ile Phe Pro Arg Ser Phe Ile Lys Asp Asp Ser Ile 
1               5                   10                  15      


Asp Asn Lys Val Leu Val Ile Lys Lys Met Asn 
            20                  25          


<210>  137
<211>  27
<212>  PRT
<213>  Olsenella uli

<400>  137

Glu Val Asp His Ile Ile Pro Arg Ser Tyr Ile Lys Asp Asp Ser Phe 
1               5                   10                  15      


Glu Asn Lys Val Leu Val Tyr Arg Glu Glu Asn 
            20                  25          


<210>  138
<211>  27
<212>  PRT
<213>  Bifidobacterium bifidum S17

<400>  138

Asp Ile Asp His Ile Ile Pro Gln Ala Val Thr Gln Asn Asp Ser Ile 
1               5                   10                  15      


Asp Asn Arg Val Leu Val Ala Arg Ala Glu Asn 
            20                  25          


<210>  139
<211>  27
<212>  PRT
<213>  Mycoplasma gallisepticum str. F

<400>  139

Glu Ile Asp His Ile Ile Pro Tyr Ser Ile Ser Phe Asp Asp Ser Ser 
1               5                   10                  15      


Ser Asn Lys Leu Leu Val Leu Ala Glu Ser Asn 
            20                  25          


<210>  140
<211>  27
<212>  PRT
<213>  Mycoplasma canis PG 14

<400>  140

Glu Ile Asp His Ile Ile Pro Tyr Ser Leu Cys Phe Asp Asp Ser Ser 
1               5                   10                  15      


Ala Asn Lys Val Leu Val His Lys Gln Ser Asn 
            20                  25          


<210>  141
<211>  27
<212>  PRT
<213>  Ilyobacter polytropus DSM 2926

<400>  141

Asp Ile Asp His Ile Ile Pro Tyr Ser Arg Ser Met Asp Asp Ser Tyr 
1               5                   10                  15      


Ser Asn Lys Val Leu Val Leu Ser Gly Glu Asn 
            20                  25          


<210>  142
<211>  27
<212>  PRT
<213>  Uncultured Termite group 1 bacterium

<400>  142

Asp Ile Asp His Ile Ile Pro Tyr Ser Lys Ser Met Asp Asp Ser Phe 
1               5                   10                  15      


Asn Asn Lys Val Leu Cys Leu Ala Glu Glu Asn 
            20                  25          


<210>  143
<211>  27
<212>  PRT
<213>  Campylobacter jejuni

<400>  143

Glu Ile Asp His Ile Tyr Pro Tyr Ser Arg Ser Phe Asp Asp Ser Tyr 
1               5                   10                  15      


Met Asn Lys Val Leu Val Phe Thr Lys Gln Asn 
            20                  25          


<210>  144
<211>  27
<212>  PRT
<213>  Clostridium cellulolyticum H10

<400>  144

Gln Ile Asp His Ile Tyr Pro Tyr Ser Arg Ser Met Asp Asp Ser Tyr 
1               5                   10                  15      


Met Asn Lys Val Leu Val Leu Thr Asp Glu Asn 
            20                  25          


<210>  145
<211>  27
<212>  PRT
<213>  Clostridium perfringens

<400>  145

Glu Ile Asp His Ile Ile Pro Phe Ser Arg Ser Phe Asp Asp Ser Leu 
1               5                   10                  15      


Ser Asn Lys Ile Leu Val Leu Gly Ser Glu Asn 
            20                  25          


<210>  146
<211>  27
<212>  PRT
<213>  N. meningitides

<400>  146

Glu Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe 
1               5                   10                  15      


Asn Asn Lys Val Leu Val Leu Gly Ser Glu Asn 
            20                  25          


<210>  147
<211>  27
<212>  PRT
<213>  Pasteurella multocida str. Pm70

<400>  147

Glu Ile Asp His Ala Leu Pro Phe Ser Arg Thr Trp Asp Asp Ser Phe 
1               5                   10                  15      


Asn Asn Lys Val Leu Val Leu Ala Ser Glu Asn 
            20                  25          


<210>  148
<211>  27
<212>  PRT
<213>  Enterococcus faecalis TX0012

<400>  148

Glu Ile Asp His Ile Ile Pro Ile Ser Ile Ser Leu Asp Asp Ser Ile 
1               5                   10                  15      


Asn Asn Lys Val Leu Val Leu Ser Lys Ala Asn 
            20                  25          


<210>  149
<211>  27
<212>  PRT
<213>  Eubacterium dolichum DSM 3991

<400>  149

Glu Val Asp His Ile Ile Pro Ile Ser Ile Ser Leu Asp Asp Ser Ile 
1               5                   10                  15      


Thr Asn Lys Val Leu Val Thr His Arg Glu Asn 
            20                  25          


<210>  150
<211>  27
<212>  PRT
<213>  Acidovorax ebreus

<400>  150

Gln Val Asp His Ala Leu Pro Tyr Ser Arg Ser Tyr Asp Asp Ser Lys 
1               5                   10                  15      


Asn Asn Lys Val Leu Val Leu Thr His Glu Asn 
            20                  25          


<210>  151
<211>  27
<212>  PRT
<213>  Streptococcus thermophilus LMD-9

<400>  151

Glu Val Asp His Ile Leu Pro Leu Ser Ile Thr Phe Asp Asp Ser Leu 
1               5                   10                  15      


Ala Asn Lys Val Leu Val Tyr Ala Thr Ala Asn 
            20                  25          


<210>  152
<211>  27
<212>  PRT
<213>  Eubacterium rectale

<400>  152

Glu Ile Asp His Ile Ile Pro Arg Ser Ile Ser Phe Asp Asp Ala Arg 
1               5                   10                  15      


Ser Asn Lys Val Leu Val Tyr Arg Ser Glu Asn 
            20                  25          


<210>  153
<211>  27
<212>  PRT
<213>  Staphylococcus lugdunensis M23590

<400>  153

Glu Val Asp His Ile Ile Pro Arg Ser Val Ser Phe Asp Asn Ser Tyr 
1               5                   10                  15      


His Asn Lys Val Leu Val Lys Gln Ser Glu Asn 
            20                  25          


<210>  154
<211>  27
<212>  PRT
<213>  Roseburia intestinalis

<400>  154

Asp Ile Asp His Ile Leu Pro Tyr Ser Ile Thr Phe Asp Asp Ser Phe 
1               5                   10                  15      


Arg Asn Lys Val Leu Val Thr Ser Gln Glu Asn 
            20                  25          


<210>  155
<211>  27
<212>  PRT
<213>  Wolinella succinogenes DSM 1740

<400>  155

Glu Ile Asp His Ile Leu Pro Arg Ser Arg Ser Ala Asp Asp Ser Phe 
1               5                   10                  15      


Ala Asn Lys Val Leu Cys Leu Ala Arg Ala Asn 
            20                  25          


<210>  156
<211>  27
<212>  PRT
<213>  Cand. Puniceispirillum marinum

<400>  156

Glu Ile Glu His Leu Leu Pro Phe Ser Leu Thr Leu Asp Asp Ser Met 
1               5                   10                  15      


Ala Asn Lys Thr Val Cys Phe Arg Gln Ala Asn 
            20                  25          


<210>  157
<211>  27
<212>  PRT
<213>  Azospirillum sp. B510

<400>  157

Asp Ile Asp His Ile Leu Pro Phe Ser Val Ser Leu Asp Asp Ser Ala 
1               5                   10                  15      


Ala Asn Lys Val Val Cys Leu Arg Glu Ala Asn 
            20                  25          


<210>  158
<211>  27
<212>  PRT
<213>  Bradyrhizobium sp. BTAi1

<400>  158

Asp Ile Asp His Leu Ile Pro Phe Ser Ile Ser Trp Asp Asp Ser Ala 
1               5                   10                  15      


Ala Asn Lys Val Val Cys Met Arg Tyr Ala Asn 
            20                  25          


<210>  159
<211>  27
<212>  PRT
<213>  Nitrobacter hamburgensis X14

<400>  159

Asp Ile Asp His Ile Leu Pro Val Ala Met Thr Leu Asp Asp Ser Pro 
1               5                   10                  15      


Ala Asn Lys Ile Ile Cys Met Arg Tyr Ala Asn 
            20                  25          


<210>  160
<211>  27
<212>  PRT
<213>  Dinoroseobacter shibae

<400>  160

Asp Val Asp His Ile Leu Pro Tyr Ser Arg Thr Leu Asp Asp Ser Phe 
1               5                   10                  15      


Pro Asn Arg Thr Leu Cys Leu Arg Glu Ala Asn 
            20                  25          


<210>  161
<211>  27
<212>  PRT
<213>  Verminephrobacter eiseniae

<400>  161

Glu Ile Glu His Ile Leu Pro Phe Ser Arg Thr Leu Asp Asp Ser Leu 
1               5                   10                  15      


Asn Asn Arg Thr Val Ala Met Arg Arg Ala Asn 
            20                  25          


<210>  162
<211>  27
<212>  PRT
<213>  Lactobacillus coryniformis KCTC 3535

<400>  162

Glu Val Asp His Ile Ile Pro Tyr Ser Ile Ser Trp Asp Asp Ser Tyr 
1               5                   10                  15      


Thr Asn Lys Val Leu Thr Ser Ala Lys Cys Asn 
            20                  25          


<210>  163
<211>  27
<212>  PRT
<213>  Rhodopseudomonas palustris

<400>  163

Gln Val Asp His Ile Leu Pro Trp Ser Arg Phe Gly Asp Asp Ser Tyr 
1               5                   10                  15      


Leu Asn Lys Thr Leu Cys Thr Ala Arg Ser Asn 
            20                  25          


<210>  164
<211>  27
<212>  PRT
<213>  Ralstonia syzygii R24

<400>  164

Gln Val Asp His Ile Leu Pro Phe Ser Lys Thr Leu Asp Asp Ser Phe 
1               5                   10                  15      


Ala Asn Lys Val Leu Ala Gln His Asp Ala Asn 
            20                  25          


<210>  165
<211>  27
<212>  PRT
<213>  Helicobacter mustelae 12198

<400>  165

Gln Ile Asp His Ala Phe Pro Leu Ser Arg Ser Leu Asp Asp Ser Gln 
1               5                   10                  15      


Ser Asn Lys Val Leu Cys Leu Thr Ser Ser Asn 
            20                  25          


<210>  166
<211>  27
<212>  PRT
<213>  Mycoplasma mobile 163K

<400>  166

Asp Ile Asp His Ile Val Pro Arg Ser Ile Ser Phe Asp Asp Ser Phe 
1               5                   10                  15      


Ser Asn Leu Val Ile Val Asn Lys Leu Asp Asn 
            20                  25          


<210>  167
<211>  27
<212>  PRT
<213>  Mycoplasma ovipneumoniae SC01

<400>  167

Glu Ile Glu His Ile Ile Pro Tyr Ser Met Ser Tyr Asp Asn Ser Gln 
1               5                   10                  15      


Ala Asn Lys Ile Leu Thr Glu Lys Ala Glu Asn 
            20                  25          


<210>  168
<211>  27
<212>  PRT
<213>  Mycoplasma synoviae 53

<400>  168

Glu Ile Asp His Val Ile Pro Tyr Ser Lys Ser Ala Asp Asp Ser Trp 
1               5                   10                  15      


Phe Asn Lys Leu Leu Val Lys Lys Ser Thr Asn 
            20                  25          


<210>  169
<211>  27
<212>  PRT
<213>  Aminomonas paucivorans DSM 12260

<400>  169

Glu Met Asp His Ile Leu Pro Tyr Ser Arg Ser Leu Asp Asn Gly Trp 
1               5                   10                  15      


His Asn Arg Val Leu Val His Gly Lys Asp Asn 
            20                  25          


<210>  170
<211>  27
<212>  PRT
<213>  Ruminococcus albus 8

<400>  170

Glu Val Asp His Ile Val Pro Tyr Ser Leu Ile Leu Asp Asn Thr Ile 
1               5                   10                  15      


Asn Asn Lys Ala Leu Val Tyr Ala Glu Glu Asn 
            20                  25          


<210>  171
<211>  27
<212>  PRT
<213>  Fibrobacter succinogenes

<400>  171

Glu Ile Glu His Val Ile Pro Gln Ser Leu Tyr Phe Asp Asp Ser Phe 
1               5                   10                  15      


Ser Asn Lys Val Ile Cys Glu Ala Glu Val Asn 
            20                  25          


<210>  172
<211>  27
<212>  PRT
<213>  Bacteroides fragilis, NCTC 9343

<400>  172

Asp Ile Glu His Ile Ile Pro Gln Ala Arg Leu Phe Asp Asp Ser Phe 
1               5                   10                  15      


Ser Asn Lys Thr Leu Glu Ala Arg Ser Val Asn 
            20                  25          


<210>  173
<211>  27
<212>  PRT
<213>  Capnocytophaga sputigena

<400>  173

Glu Ile Glu His Ile Val Pro Lys Ala Arg Val Phe Asp Asp Ser Phe 
1               5                   10                  15      


Ser Asn Lys Thr Leu Thr Phe His Arg Ile Asn 
            20                  25          


<210>  174
<211>  28
<212>  PRT
<213>  Finegoldia magna

<400>  174

Asp Lys Asp His Ile Ile Pro Gln Ser Met Lys Lys Asp Asp Ser Ile 
1               5                   10                  15      


Ile Asn Asn Leu Val Leu Val Asn Lys Asn Ala Asn 
            20                  25              


<210>  175
<211>  27
<212>  PRT
<213>  Parvibaculum lavamentivorans DS-1

<400>  175

Glu Val Glu His Ile Trp Pro Arg Ser Arg Ser Phe Asp Asn Ser Pro 
1               5                   10                  15      


Arg Asn Lys Thr Leu Cys Arg Lys Asp Val Asn 
            20                  25          


<210>  176
<211>  27
<212>  PRT
<213>  Bacillus cereus

<400>  176

Ile Val Asn His Ile Ile Pro Tyr Asn Arg Ser Phe Asp Asp Thr Tyr 
1               5                   10                  15      


His Asn Arg Val Leu Thr Leu Thr Glu Thr Lys 
            20                  25          


<210>  177
<211>  27
<212>  PRT
<213>  Prevotella micans

<400>  177

Asp Met Glu His Thr Ile Pro Lys Ser Ile Ser Phe Asp Asn Ser Asp 
1               5                   10                  15      


Gln Asn Leu Thr Leu Cys Glu Ser Tyr Tyr Asn 
            20                  25          


<210>  178
<211>  27
<212>  PRT
<213>  Prevotella ruminicola

<400>  178

Asp Ile Glu His Thr Ile Pro Arg Ser Ala Gly Gly Asp Ser Thr Lys 
1               5                   10                  15      


Met Asn Leu Thr Leu Cys Ser Ser Arg Phe Asn 
            20                  25          


<210>  179
<211>  27
<212>  PRT
<213>  Flavobacterium columnare

<400>  179

Asp Ile Glu His Thr Ile Pro Arg Ser Ile Ser Gln Asp Asn Ser Gln 
1               5                   10                  15      


Met Asn Lys Thr Leu Cys Ser Leu Lys Phe Asn 
            20                  25          


<210>  180
<211>  27
<212>  PRT
<213>  Rhodospirillum rubrum

<400>  180

Asp Ile Asp His Val Ile Pro Leu Ala Arg Gly Gly Arg Asp Ser Leu 
1               5                   10                  15      


Asp Asn Met Val Leu Cys Gln Ser Asp Ala Asn 
            20                  25          


<210>  181
<211>  27
<212>  PRT
<213>  Elusimicrobium minutum Pei191

<400>  181

Asp Ile Glu His Leu Phe Pro Ile Ala Glu Ser Glu Asp Asn Gly Arg 
1               5                   10                  15      


Asn Asn Leu Val Ile Ser His Ser Ala Cys Asn 
            20                  25          


<210>  182
<211>  27
<212>  PRT
<213>  Sphaerochaeta globus str. Buddy

<400>  182

Asp Val Asp His Ile Phe Pro Arg Asp Asp Thr Ala Asp Asn Ser Tyr 
1               5                   10                  15      


Gly Asn Lys Val Val Ala His Arg Gln Cys Asn 
            20                  25          


<210>  183
<211>  27
<212>  PRT
<213>  Nitratifractor salsuginis DSM 16511

<400>  183

Asp Ile Glu His Ile Val Pro Gln Ser Leu Gly Gly Leu Ser Thr Asp 
1               5                   10                  15      


Tyr Asn Thr Ile Val Thr Leu Lys Ser Val Asn 
            20                  25          


<210>  184
<211>  27
<212>  PRT
<213>  Acidothermus cellulolyticus 11B

<400>  184

Glu Leu Asp His Ile Val Pro Arg Thr Asp Gly Gly Ser Asn Arg His 
1               5                   10                  15      


Glu Asn Leu Ala Ile Thr Cys Gly Ala Cys Asn 
            20                  25          


<210>  185
<211>  28
<212>  PRT
<213>  Bifidobacterium longum DJO10A

<400>  185

Glu Met Asp His Ile Val Pro Arg Lys Gly Val Gly Ser Thr Asn Thr 
1               5                   10                  15      


Arg Thr Asn Phe Ala Ala Val Cys Ala Glu Cys Asn 
            20                  25              


<210>  186
<211>  28
<212>  PRT
<213>  Bifidobacterium dentium

<400>  186

Glu Met Asp His Ile Val Pro Arg Lys Gly Val Gly Ser Thr Asn Thr 
1               5                   10                  15      


Arg Val Asn Leu Ala Ala Ala Cys Ala Ala Cys Asn 
            20                  25              


<210>  187
<211>  28
<212>  PRT
<213>  Corynebacterium diphtheriae

<400>  187

Glu Met Asp His Ile Val Pro Arg Ala Gly Gln Gly Ser Thr Asn Thr 
1               5                   10                  15      


Arg Glu Asn Leu Val Ala Val Cys His Arg Cys Asn 
            20                  25              


<210>  188
<211>  33
<212>  PRT
<213>  Sutterella wadsworthensis

<400>  188

Glu Ile Asp His Ile Leu Pro Arg Ser Leu Ile Lys Asp Ala Arg Gly 
1               5                   10                  15      


Ile Val Phe Asn Ala Glu Pro Asn Leu Ile Tyr Ala Ser Ser Arg Gly 
            20                  25                  30          


Asn 
    


<210>  189
<211>  33
<212>  PRT
<213>  Gamma proteobacterium HTCC5015

<400>  189

Glu Ile Asp His Ile Ile Pro Arg Ser Leu Thr Gly Arg Thr Lys Lys 
1               5                   10                  15      


Thr Val Phe Asn Ser Glu Ala Asn Leu Ile Tyr Cys Ser Ser Lys Gly 
            20                  25                  30          


Asn 
    


<210>  190
<211>  33
<212>  PRT
<213>  Parasutterella excrementihominis

<400>  190

Glu Ile Asp His Ile Ile Pro Arg Ser Leu Thr Leu Lys Lys Ser Glu 
1               5                   10                  15      


Ser Ile Tyr Asn Ser Glu Val Asn Leu Ile Phe Val Ser Ala Gln Gly 
            20                  25                  30          


Asn 
    


<210>  191
<211>  33
<212>  PRT
<213>  Legionella pneumophila str. Paris

<400>  191

Glu Ile Asp His Ile Tyr Pro Arg Ser Leu Ser Lys Lys His Phe Gly 
1               5                   10                  15      


Val Ile Phe Asn Ser Glu Val Asn Leu Ile Tyr Cys Ser Ser Gln Gly 
            20                  25                  30          


Asn 
    


<210>  192
<211>  33
<212>  PRT
<213>  Wolinella succinogenes DSM 1740

<400>  192

Glu Ile Asp His Ile Leu Pro Arg Ser His Thr Leu Lys Ile Tyr Gly 
1               5                   10                  15      


Thr Val Phe Asn Pro Glu Gly Asn Leu Ile Tyr Val His Gln Lys Cys 
            20                  25                  30          


Asn 
    


<210>  193
<211>  30
<212>  PRT
<213>  Francisella tularensis

<400>  193

Glu Leu Asp His Ile Ile Pro Arg Ser His Lys Lys Tyr Gly Thr Leu 
1               5                   10                  15      


Asn Asp Glu Ala Asn Leu Ile Cys Val Thr Arg Gly Asp Asn 
            20                  25                  30  


<210>  194
<211>  27
<212>  PRT
<213>  Akkermansia muciniphila

<400>  194

Glu Leu Glu His Ile Val Pro His Ser Phe Arg Gln Ser Asn Ala Leu 
1               5                   10                  15      


Ser Ser Leu Val Leu Thr Trp Pro Gly Val Asn 
            20                  25          


<210>  195
<211>  27
<212>  PRT
<213>  Solobacterium moorei F0204

<400>  195

Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Ile Lys Asp Asp Ser Ile 
1               5                   10                  15      


Thr Asn Arg Val Leu Val Glu Lys Asp Ile Asn 
            20                  25          


<210>  196
<211>  28
<212>  PRT
<213>  Veillonella atypica ACS-134-V-Col7a

<400>  196

Tyr Asp Ile Asp His Ile Tyr Pro Arg Ser Leu Thr Lys Asp Asp Ser 
1               5                   10                  15      


Phe Asp Asn Leu Val Leu Cys Glu Arg Thr Ala Asn 
            20                  25              


<210>  197
<211>  28
<212>  PRT
<213>  Fusobacterium nucleatum

<400>  197

Asp Ile Asp His Ile Tyr Pro Arg Ser Lys Val Ile Lys Asp Asp Ser 
1               5                   10                  15      


Phe Asp Asn Leu Val Leu Val Leu Lys Asn Glu Asn 
            20                  25              


<210>  198
<211>  27
<212>  PRT
<213>  Filifactor alocis

<400>  198

Asp Arg Asp His Ile Tyr Pro Gln Ser Lys Ile Lys Asp Asp Ser Ile 
1               5                   10                  15      


Asp Asn Leu Val Leu Val Asn Lys Thr Tyr Asn 
            20                  25          


<210>  199
<211>  5
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Any nucleotide (e.g., A, G, C, or T)

<400>  199
nggng                                                                    5


<210>  200
<211>  7
<212>  DNA
<213>  Streptococcus thermophilus


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  A or T

<400>  200
nnagaaw                                                                  7


<210>  201
<211>  4
<212>  DNA
<213>  Streptococcus mutans


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  A or G

<400>  201
naar                                                                     4


<210>  202
<211>  5
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  A or G

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  A or G

<400>  202
nngrr                                                                    5


<210>  203
<211>  6
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  A or G

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  A or G

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  Any nucleotide (e.g., A, G, C, or T)

<400>  203
nngrrn                                                                   6


<210>  204
<211>  6
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  A or G

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  A or G

<400>  204
nngrrt                                                                   6


<210>  205
<211>  6
<212>  DNA
<213>  Staphylococcus aureus


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Any nucleotide (e.g., A, G, C, or T)

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  A or G

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  A or G

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  A, G, or C

<400>  205
nngrrv                                                                   6


<210>  206

<400>  206
000

<210>  207

<400>  207
000

<210>  208

<400>  208
000

<210>  209

<400>  209
000

<210>  210

<400>  210
000

<210>  211

<400>  211
000

<210>  212

<400>  212
000

<210>  213

<400>  213
000

<210>  214

<400>  214
000

<210>  215

<400>  215
000

<210>  216

<400>  216
000

<210>  217

<400>  217
000

<210>  218

<400>  218
000

<210>  219

<400>  219
000

<210>  220

<400>  220
000

<210>  221

<400>  221
000

<210>  222

<400>  222
000

<210>  223

<400>  223
000

<210>  224

<400>  224
000

<210>  225

<400>  225
000

<210>  226

<400>  226
000

<210>  227

<400>  227
000

<210>  228

<400>  228
000

<210>  229

<400>  229
000

<210>  230

<400>  230
000

<210>  231

<400>  231
000

<210>  232

<400>  232
000

<210>  233

<400>  233
000

<210>  234

<400>  234
000

<210>  235

<400>  235
000

<210>  236

<400>  236
000

<210>  237

<400>  237
000

<210>  238

<400>  238
000

<210>  239

<400>  239
000

<210>  240

<400>  240
000

<210>  241

<400>  241
000

<210>  242

<400>  242
000

<210>  243

<400>  243
000

<210>  244

<400>  244
000

<210>  245

<400>  245
000

<210>  246

<400>  246
000

<210>  247

<400>  247
000

<210>  248

<400>  248
000

<210>  249

<400>  249
000

<210>  250

<400>  250
000

<210>  251
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  251
gaaggaaacu agcuaaa                                                      17


<210>  252
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  252
ggagaaggaa acuagcuaaa                                                   20


<210>  253
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  253
gggagaagga aacuagcuaa                                                   20


<210>  254
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  254
guauccucua ugaugggaga                                                   20


<210>  255
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  255
guuuccuucu cccaucauag                                                   20


<210>  256
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  256
guccugguau ccucuaugau                                                   20


<210>  257
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  257
agaaggaaac uagcuaa                                                      17


<210>  258
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  258
uccucuauga ugggaga                                                      17


<210>  259
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  259
ccugguaucc ucuauga                                                      17


<210>  260
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  260
ccaucauaga ggauacc                                                      17


<210>  261
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  261
uccuucuccc aucauag                                                      17


<210>  262
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  262
uagcaguauc cucuugg                                                      17


<210>  263
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  263
uuagcaguau ccucuug                                                      17


<210>  264
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  264
aacuggaaug acugaau                                                      17


<210>  265
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  265
cugguauccu cuaugau                                                      17


<210>  266
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  266
aauuagcagu auccucu                                                      17


<210>  267
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  267
auuagcagua uccucuu                                                      17


<210>  268
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  268
aguccuggua uccucuauga                                                   20


<210>  269
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  269
cucccaucau agaggauacc                                                   20


<210>  270
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  270
aauuagcagu auccucuugg                                                   20


<210>  271
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  271
aaauuagcag uauccucuug                                                   20


<210>  272
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  272
aaaaacugga augacugaau                                                   20


<210>  273
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  273
aaaaauuagc aguauccucu                                                   20


<210>  274
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  274
aaaauuagca guauccucuu                                                   20


<210>  275
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  275
gaaucggaac aaggcaa                                                      17


<210>  276
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  276
gaccaauagc cuugaca                                                      17


<210>  277
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  277
ggcuauuggu caaggca                                                      17


<210>  278
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  278
gucaaggcua uugguca                                                      17


<210>  279
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  279
guguguggaa cugcuga                                                      17


<210>  280
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  280
gggccggcgg cuggcua                                                      17


<210>  281
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  281
gaguauccag ugaggcc                                                      17


<210>  282
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  282
gcugacaaaa gaagucc                                                      17


<210>  283
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  283
ggccaggggc cggcggc                                                      17


<210>  284
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  284
gggaaggggc ccccaag                                                      17


<210>  285
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  285
gagauagugu ggggaag                                                      17


<210>  286
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  286
guauccagug aggccag                                                      17


<210>  287
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  287
gugaggccag gggccgg                                                      17


<210>  288
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  288
gcuggccaac ccauggg                                                      17


<210>  289
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  289
ggcuaaacuc cacccau                                                      17


<210>  290
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  290
ggauacucua agacuau                                                      17


<210>  291
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  291
ggggccggcg gcuggcu                                                      17


<210>  292
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  292
ggcuagggau gaagaauaaa                                                   20


<210>  293
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  293
gagugugugg aacugcugaa                                                   20


<210>  294
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  294
ggaaugacug aaucggaaca                                                   20


<210>  295
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  295
gcauugagau agugugggga                                                   20


<210>  296
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  296
gcuauugguc aaggcaaggc                                                   20


<210>  297
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  297
guggggaagg ggcccccaag                                                   20


<210>  298
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  298
ggcaaggcug gccaacccau                                                   20


<210>  299
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  299
guuugccuug ucaaggcuau                                                   20


<210>  300
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  300
gcuaaacucc acccaugggu                                                   20


<210>  301
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  301
caaauaucug ucugaaa                                                      17


<210>  302
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  302
uagggaugaa gaauaaa                                                      17


<210>  303
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  303
ugagauagug uggggaa                                                      17


<210>  304
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  304
uguguggaac ugcugaa                                                      17


<210>  305
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  305
augacugaau cggaaca                                                      17


<210>  306
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  306
caaggcuggc caaccca                                                      17


<210>  307
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  307
uggcuaaacu ccaccca                                                      17


<210>  308
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  308
uggguggagu uuagcca                                                      17


<210>  309
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  309
aguauccagu gaggcca                                                      17


<210>  310
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  310
ucaaguuugc cuuguca                                                      17


<210>  311
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  311
uugagauagu gugggga                                                      17


<210>  312
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  312
auaaauuaga gaaaaac                                                      17


<210>  313
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  313
ccggccccug gccucac                                                      17


<210>  314
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  314
agccagccgc cggcccc                                                      17


<210>  315
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  315
cugucugaaa cgguccc                                                      17


<210>  316
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  316
auggguggag uuuagcc                                                      17


<210>  317
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  317
caucccuagc cagccgc                                                      17


<210>  318
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  318
auuggucaag gcaaggc                                                      17


<210>  319
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  319
ccagugaggc caggggc                                                      17


<210>  320
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  320
uuccacacac ucgcuuc                                                      17


<210>  321
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  321
cgcuucugga acgucug                                                      17


<210>  322
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  322
ucuuagagua uccagug                                                      17


<210>  323
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  323
uuugcauuga gauagug                                                      17


<210>  324
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  324
uuccagaagc gagugug                                                      17


<210>  325
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  325
ugcauugaga uagugug                                                      17


<210>  326
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  326
aaggcuggcc aacccau                                                      17


<210>  327
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  327
ugccuuguca aggcuau                                                      17


<210>  328
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  328
aaacuccacc caugggu                                                      17


<210>  329
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  329
uugcauugag auagugu                                                      17


<210>  330
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  330
augcaaauau cugucugaaa                                                   20


<210>  331
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  331
acugaaucgg aacaaggcaa                                                   20


<210>  332
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  332
cauugagaua guguggggaa                                                   20


<210>  333
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  333
cuugaccaau agccuugaca                                                   20


<210>  334
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  334
aggcaaggcu ggccaaccca                                                   20


<210>  335
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  335
cccuggcuaa acuccaccca                                                   20


<210>  336
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  336
ccaugggugg aguuuagcca                                                   20


<210>  337
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  337
uagaguaucc agugaggcca                                                   20


<210>  338
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  338
caaggcuauu ggucaaggca                                                   20


<210>  339
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  339
cuugucaagg cuauugguca                                                   20


<210>  340
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  340
uggucaaguu ugccuuguca                                                   20


<210>  341
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  341
cgagugugug gaacugcuga                                                   20


<210>  342
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  342
caggggccgg cggcuggcua                                                   20


<210>  343
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  343
agaauaaauu agagaaaaac                                                   20


<210>  344
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  344
ccgccggccc cuggccucac                                                   20


<210>  345
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  345
ccuagccagc cgccggcccc                                                   20


<210>  346
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  346
uaucugucug aaacgguccc                                                   20


<210>  347
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  347
cccaugggug gaguuuagcc                                                   20


<210>  348
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  348
uuagaguauc cagugaggcc                                                   20


<210>  349
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  349
acggcugaca aaagaagucc                                                   20


<210>  350
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  350
cuucaucccu agccagccgc                                                   20


<210>  351
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  351
ugaggccagg ggccggcggc                                                   20


<210>  352
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  352
uauccaguga ggccaggggc                                                   20


<210>  353
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  353
caguuccaca cacucgcuuc                                                   20


<210>  354
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  354
auugagauag uguggggaag                                                   20


<210>  355
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  355
agaguaucca gugaggccag                                                   20


<210>  356
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  356
ccagugaggc caggggccgg                                                   20


<210>  357
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  357
aaggcuggcc aacccauggg                                                   20


<210>  358
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  358
acucgcuucu ggaacgucug                                                   20


<210>  359
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  359
uagucuuaga guauccagug                                                   20


<210>  360
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  360
auauuugcau ugagauagug                                                   20


<210>  361
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  361
acguuccaga agcgagugug                                                   20


<210>  362
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  362
auuugcauug agauagugug                                                   20


<210>  363
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  363
ccuggcuaaa cuccacccau                                                   20


<210>  364
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  364
acuggauacu cuaagacuau                                                   20


<210>  365
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  365
ccaggggccg gcggcuggcu                                                   20


<210>  366
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  366
uauuugcauu gagauagugu                                                   20


<210>  367
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  367
guuuccuucu cccaucaua                                                    19


<210>  368
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  368
gcuaguuucc uucucccauc aua                                               23


<210>  369
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  369
gaauaaauua gagaaaaac                                                    19


<210>  370
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  370
gaagaauaaa uuagagaaaa ac                                                22


<210>  371
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  371
ggaagaauaa auuagagaaa aac                                               23


<210>  372
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  372
gggaagaaua aauuagagaa aaac                                              24


<210>  373
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  373
gaaggaaacu agcuaaaggg                                                   20


<210>  374
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  374
gagaaggaaa cuagcuaaag gg                                                22


<210>  375
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  375
ggagaaggaa acuagcuaaa ggg                                               23


<210>  376
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  376
gggagaagga aacuagcuaa aggg                                              24


<210>  377
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  377
uuuccuucuc ccaucaua                                                     18


<210>  378
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  378
aguuuccuuc ucccaucaua                                                   20


<210>  379
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  379
uaguuuccuu cucccaucau a                                                 21


<210>  380
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  380
cuaguuuccu ucucccauca ua                                                22


<210>  381
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  381
agcuaguuuc cuucucccau caua                                              24


<210>  382
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  382
agagaaaaac uggaauga                                                     18


<210>  383
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  383
uagagaaaaa cuggaauga                                                    19


<210>  384
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  384
uuagagaaaa acuggaauga                                                   20


<210>  385
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  385
auuagagaaa aacuggaaug a                                                 21


<210>  386
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  386
aauuagagaa aaacuggaau ga                                                22


<210>  387
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  387
aaauuagaga aaaacuggaa uga                                               23


<210>  388
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  388
uaaauuagag aaaaacugga auga                                              24


<210>  389
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  389
aauaaauuag agaaaaac                                                     18


<210>  390
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  390
aagaauaaau uagagaaaaa c                                                 21


<210>  391
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  391
aggaaacuag cuaaaggg                                                     18


<210>  392
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  392
aaggaaacua gcuaaaggg                                                    19


<210>  393
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  393
agaaggaaac uagcuaaagg g                                                 21


<210>  394
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  394
uggggaaggg gcccccaa                                                     18


<210>  395
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  395
guggggaagg ggcccccaa                                                    19


<210>  396
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  396
uguggggaag gggcccccaa                                                   20


<210>  397
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  397
guguggggaa ggggccccca a                                                 21


<210>  398
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  398
agugugggga aggggccccc aa                                                22


<210>  399
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  399
uagugugggg aaggggcccc caa                                               23


<210>  400
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  400
auaguguggg gaaggggccc ccaa                                              24


<210>  401
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  401
accucagacg uuccagaa                                                     18


<210>  402
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  402
aaccucagac guuccagaa                                                    19


<210>  403
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  403
uaaccucaga cguuccagaa                                                   20


<210>  404
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  404
auaaccucag acguuccaga a                                                 21


<210>  405
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  405
gauaaccuca gacguuccag aa                                                22


<210>  406
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  406
ugauaaccuc agacguucca gaa                                               23


<210>  407
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  407
uugauaaccu cagacguucc agaa                                              24


<210>  408
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  408
cgccggcccc uggccuca                                                     18


<210>  409
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  409
ccgccggccc cuggccuca                                                    19


<210>  410
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  410
gccgccggcc ccuggccuca                                                   20


<210>  411
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  411
agccgccggc cccuggccuc a                                                 21


<210>  412
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  412
cagccgccgg ccccuggccu ca                                                22


<210>  413
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  413
ccagccgccg gccccuggcc uca                                               23


<210>  414
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  414
gccagccgcc ggccccuggc cuca                                              24


<210>  415
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  415
ggcaaggcug gccaaccc                                                     18


<210>  416
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  416
aggcaaggcu ggccaaccc                                                    19


<210>  417
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  417
aaggcaaggc uggccaaccc                                                   20


<210>  418
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  418
caaggcaagg cuggccaacc c                                                 21


<210>  419
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  419
ucaaggcaag gcuggccaac cc                                                22


<210>  420
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  420
gucaaggcaa ggcuggccaa ccc                                               23


<210>  421
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  421
ggucaaggca aggcuggcca accc                                              24


<210>  422
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  422
ggcuggccaa cccauggg                                                     18


<210>  423
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  423
aggcuggcca acccauggg                                                    19


<210>  424
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  424
caaggcuggc caacccaugg g                                                 21


<210>  425
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  425
gcaaggcugg ccaacccaug gg                                                22


<210>  426
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  426
ggcaaggcug gccaacccau ggg                                               23


<210>  427
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  427
aggcaaggcu ggccaaccca uggg                                              24


<210>  428
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  428
gagugugugg aacugcug                                                     18


<210>  429
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  429
cgagugugug gaacugcug                                                    19


<210>  430
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  430
gcgagugugu ggaacugcug                                                   20


<210>  431
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  431
agcgagugug uggaacugcu g                                                 21


<210>  432
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  432
aagcgagugu guggaacugc ug                                                22


<210>  433
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  433
gaagcgagug uguggaacug cug                                               23


<210>  434
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  434
agaagcgagu guguggaacu gcug                                              24


<210>  435
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  435
ccuggcuaaa cuccaccc                                                     18


<210>  436
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  436
cccuggcuaa acuccaccc                                                    19


<210>  437
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  437
ucccuggcua aacuccaccc                                                   20


<210>  438
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  438
gucccuggcu aaacuccacc c                                                 21


<210>  439
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  439
ggucccuggc uaaacuccac cc                                                22


<210>  440
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  440
cggucccugg cuaaacucca ccc                                               23


<210>  441
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  441
acggucccug gcuaaacucc accc                                              24


<210>  442
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  442
ggcggcuggc uagggaug                                                     18


<210>  443
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  443
cggcggcugg cuagggaug                                                    19


<210>  444
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  444
ccggcggcug gcuagggaug                                                   20


<210>  445
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  445
gccggcggcu ggcuagggau g                                                 21


<210>  446
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  446
ggccggcggc uggcuaggga ug                                                22


<210>  447
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  447
gggccggcgg cuggcuaggg aug                                               23


<210>  448
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  448
ggggccggcg gcuggcuagg gaug                                              24


<210>  449
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  449
aggggccggc ggcuggcu                                                     18


<210>  450
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  450
caggggccgg cggcuggcu                                                    19


<210>  451
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  451
gccaggggcc ggcggcuggc u                                                 21


<210>  452
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  452
ggccaggggc cggcggcugg cu                                                22


<210>  453
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  453
aggccagggg ccggcggcug gcu                                               23


<210>  454
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  454
gaggccaggg gccggcggcu ggcu                                              24


<210>  455
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  455
aaacuugacc aauagucu                                                     18


<210>  456
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  456
caaacuugac caauagucu                                                    19


<210>  457
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  457
gcaaacuuga ccaauagucu                                                   20


<210>  458
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  458
ggcaaacuug accaauaguc u                                                 21


<210>  459
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  459
aggcaaacuu gaccaauagu cu                                                22


<210>  460
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  460
aaggcaaacu ugaccaauag ucu                                               23


<210>  461
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  461
caaggcaaac uugaccaaua gucu                                              24


<210>  462
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  462
uucagacaga uauuugca                                                     18


<210>  463
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  463
uuucagacag auauuugca                                                    19


<210>  464
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  464
guuucagaca gauauuugca                                                   20


<210>  465
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  465
cguuucagac agauauuugc a                                                 21


<210>  466
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  466
ccguuucaga cagauauuug ca                                                22


<210>  467
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  467
accguuucag acagauauuu gca                                               23


<210>  468
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  468
gaccguuuca gacagauauu ugca                                              24


<210>  469
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  469
aguuuccuuc ucccauca                                                     18


<210>  470
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  470
uaguuuccuu cucccauca                                                    19


<210>  471
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  471
cuaguuuccu ucucccauca                                                   20


<210>  472
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  472
gcuaguuucc uucucccauc a                                                 21


<210>  473
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  473
agcuaguuuc cuucucccau ca                                                22


<210>  474
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  474
uagcuaguuu ccuucuccca uca                                               23


<210>  475
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  475
uuagcuaguu uccuucuccc auca                                              24


<210>  476
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  476
auugagauag ugugggga                                                     18


<210>  477
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  477
cauugagaua gugugggga                                                    19


<210>  478
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  478
ugcauugaga uagugugggg a                                                 21


<210>  479
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  479
uugcauugag auaguguggg ga                                                22


<210>  480
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  480
uuugcauuga gauagugugg gga                                               23


<210>  481
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  481
auuugcauug agauagugug ggga                                              24


<210>  482
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  482
ucccaucaua gaggauac                                                     18


<210>  483
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  483
cucccaucau agaggauac                                                    19


<210>  484
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  484
ucucccauca uagaggauac                                                   20


<210>  485
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  485
uucucccauc auagaggaua c                                                 21


<210>  486
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  486
cuucucccau cauagaggau ac                                                22


<210>  487
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  487
ccuucuccca ucauagagga uac                                               23


<210>  488
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  488
uccuucuccc aucauagagg auac                                              24


<210>  489
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  489
uguggggaag gggccccc                                                     18


<210>  490
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  490
guguggggaa ggggccccc                                                    19


<210>  491
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  491
agugugggga aggggccccc                                                   20


<210>  492
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  492
uagugugggg aaggggcccc c                                                 21


<210>  493
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  493
auaguguggg gaaggggccc cc                                                22


<210>  494
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  494
gauagugugg ggaaggggcc ccc                                               23


<210>  495
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  495
agauagugug gggaaggggc cccc                                              24


<210>  496
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  496
caugggugga guuuagcc                                                     18


<210>  497
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  497
ccaugggugg aguuuagcc                                                    19


<210>  498
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  498
acccaugggu ggaguuuagc c                                                 21


<210>  499
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  499
aacccauggg uggaguuuag cc                                                22


<210>  500
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  500
caacccaugg guggaguuua gcc                                               23


<210>  501
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  501
ccaacccaug gguggaguuu agcc                                              24


<210>  502
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  502
ccaugggugg aguuuagc                                                     18


<210>  503
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  503
cccaugggug gaguuuagc                                                    19


<210>  504
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  504
acccaugggu ggaguuuagc                                                   20


<210>  505
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  505
aacccauggg uggaguuuag c                                                 21


<210>  506
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  506
caacccaugg guggaguuua gc                                                22


<210>  507
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  507
ccaacccaug gguggaguuu agc                                               23


<210>  508
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  508
gccaacccau ggguggaguu uagc                                              24


<210>  509
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  509
ugauaaccuc agacguuc                                                     18


<210>  510
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  510
uugauaaccu cagacguuc                                                    19


<210>  511
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  511
auugauaacc ucagacguuc                                                   20


<210>  512
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  512
uauugauaac cucagacguu c                                                 21


<210>  513
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  513
uuauugauaa ccucagacgu uc                                                22


<210>  514
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  514
cuuauugaua accucagacg uuc                                               23


<210>  515
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  515
gcuuauugau aaccucagac guuc                                              24


<210>  516
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  516
cauugagaua gugugggg                                                     18


<210>  517
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  517
gcauugagau agugugggg                                                    19


<210>  518
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  518
ugcauugaga uagugugggg                                                   20


<210>  519
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  519
uugcauugag auaguguggg g                                                 21


<210>  520
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  520
uuugcauuga gauagugugg gg                                                22


<210>  521
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  521
auuugcauug agauagugug ggg                                               23


<210>  522
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  522
uauuugcauu gagauagugu gggg                                              24


<210>  523
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  523
aggcuggcca acccaugg                                                     18


<210>  524
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  524
aaggcuggcc aacccaugg                                                    19


<210>  525
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  525
caaggcuggc caacccaugg                                                   20


<210>  526
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  526
gcaaggcugg ccaacccaug g                                                 21


<210>  527
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  527
ggcaaggcug gccaacccau gg                                                22


<210>  528
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  528
aggcaaggcu ggccaaccca ugg                                               23


<210>  529
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  529
aaggcaaggc uggccaaccc augg                                              24


<210>  530
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  530
agcgagugug uggaacug                                                     18


<210>  531
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  531
aagcgagugu guggaacug                                                    19


<210>  532
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  532
gaagcgagug uguggaacug                                                   20


<210>  533
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  533
agaagcgagu guguggaacu g                                                 21


<210>  534
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  534
cagaagcgag uguguggaac ug                                                22


<210>  535
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  535
ccagaagcga guguguggaa cug                                               23


<210>  536
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  536
uccagaagcg agugugugga acug                                              24


<210>  537
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  537
auuugcauug agauagug                                                     18


<210>  538
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  538
uauuugcauu gagauagug                                                    19


<210>  539
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  539
gauauuugca uugagauagu g                                                 21


<210>  540
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  540
agauauuugc auugagauag ug                                                22


<210>  541
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  541
cagauauuug cauugagaua gug                                               23


<210>  542
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  542
acagauauuu gcauugagau agug                                              24


<210>  543
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  543
guuccagaag cgagugug                                                     18


<210>  544
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  544
cguuccagaa gcgagugug                                                    19


<210>  545
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  545
gacguuccag aagcgagugu g                                                 21


<210>  546
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  546
agacguucca gaagcgagug ug                                                22


<210>  547
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  547
cagacguucc agaagcgagu gug                                               23


<210>  548
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  548
ucagacguuc cagaagcgag ugug                                              24


<210>  549
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  549
uugcauugag auagugug                                                     18


<210>  550
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  550
uuugcauuga gauagugug                                                    19


<210>  551
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  551
uauuugcauu gagauagugu g                                                 21


<210>  552
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  552
auauuugcau ugagauagug ug                                                22


<210>  553
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  553
gauauuugca uugagauagu gug                                               23


<210>  554
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  554
agauauuugc auugagauag ugug                                              24


<210>  555
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  555
uauuugcauu gagauagu                                                     18


<210>  556
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  556
auauuugcau ugagauagu                                                    19


<210>  557
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  557
gauauuugca uugagauagu                                                   20


<210>  558
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  558
agauauuugc auugagauag u                                                 21


<210>  559
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  559
cagauauuug cauugagaua gu                                                22


<210>  560
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  560
acagauauuu gcauugagau agu                                               23


<210>  561
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  561
gacagauauu ugcauugaga uagu                                              24


<210>  562
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  562
cguuccagaa gcgagugu                                                     18


<210>  563
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  563
acguuccaga agcgagugu                                                    19


<210>  564
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  564
gacguuccag aagcgagugu                                                   20


<210>  565
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  565
agacguucca gaagcgagug u                                                 21


<210>  566
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  566
cagacguucc agaagcgagu gu                                                22


<210>  567
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  567
ucagacguuc cagaagcgag ugu                                               23


<210>  568
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  568
cucagacguu ccagaagcga gugu                                              24


<210>  569
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  569
uuugcauuga gauagugu                                                     18


<210>  570
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  570
auuugcauug agauagugu                                                    19


<210>  571
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  571
auauuugcau ugagauagug u                                                 21


<210>  572
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  572
gauauuugca uugagauagu gu                                                22


<210>  573
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  573
agauauuugc auugagauag ugu                                               23


<210>  574
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  574
cagauauuug cauugagaua gugu                                              24


<210>  575
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  575
gaauaaauua gagaaaaa                                                     18


<210>  576
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  576
agaauaaauu agagaaaaa                                                    19


<210>  577
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  577
aagaauaaau uagagaaaaa                                                   20


<210>  578
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  578
gaagaauaaa uuagagaaaa a                                                 21


<210>  579
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  579
ggaagaauaa auuagagaaa aa                                                22


<210>  580
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  580
gggaagaaua aauuagagaa aaa                                               23


<210>  581
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  581
agggaagaau aaauuagaga aaaa                                              24


<210>  582
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  582
cuagggauga agaauaaa                                                     18


<210>  583
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  583
gcuagggaug aagaauaaa                                                    19


<210>  584
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  584
uggcuaggga ugaagaauaa a                                                 21


<210>  585
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  585
cuggcuaggg augaagaaua aa                                                22


<210>  586
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  586
gcuggcuagg gaugaagaau aaa                                               23


<210>  587
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  587
ggcuggcuag ggaugaagaa uaaa                                              24


<210>  588
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  588
agaaggaaac uagcuaaa                                                     18


<210>  589
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  589
gagaaggaaa cuagcuaaa                                                    19


<210>  590
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  590
gggagaagga aacuagcuaa a                                                 21


<210>  591
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  591
ugggagaagg aaacuagcua aa                                                22


<210>  592
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  592
augggagaag gaaacuagcu aaa                                               23


<210>  593
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  593
gaugggagaa ggaaacuagc uaaa                                              24


<210>  594
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  594
aaaacuggaa ugacugaa                                                     18


<210>  595
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  595
aaaaacugga augacugaa                                                    19


<210>  596
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  596
gaaaaacugg aaugacugaa                                                   20


<210>  597
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  597
agaaaaacug gaaugacuga a                                                 21


<210>  598
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  598
gagaaaaacu ggaaugacug aa                                                22


<210>  599
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  599
agagaaaaac uggaaugacu gaa                                               23


<210>  600
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  600
uagagaaaaa cuggaaugac ugaa                                              24


<210>  601
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  601
gcuagggaug aagaauaa                                                     18


<210>  602
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  602
ggcuagggau gaagaauaa                                                    19


<210>  603
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  603
uggcuaggga ugaagaauaa                                                   20


<210>  604
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  604
cuggcuaggg augaagaaua a                                                 21


<210>  605
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  605
gcuggcuagg gaugaagaau aa                                                22


<210>  606
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  606
ggcuggcuag ggaugaagaa uaa                                               23


<210>  607
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  607
cggcuggcua gggaugaaga auaa                                              24


<210>  608
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  608
gagaaggaaa cuagcuaa                                                     18


<210>  609
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  609
ggagaaggaa acuagcuaa                                                    19


<210>  610
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  610
ugggagaagg aaacuagcua a                                                 21


<210>  611
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  611
augggagaag gaaacuagcu aa                                                22


<210>  612
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  612
gaugggagaa ggaaacuagc uaa                                               23


<210>  613
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  613
ugaugggaga aggaaacuag cuaa                                              24


<210>  614
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  614
auagucuuag aguaucca                                                     18


<210>  615
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  615
aauagucuua gaguaucca                                                    19


<210>  616
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  616
caauagucuu agaguaucca                                                   20


<210>  617
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  617
ccaauagucu uagaguaucc a                                                 21


<210>  618
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  618
accaauaguc uuagaguauc ca                                                22


<210>  619
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  619
gaccaauagu cuuagaguau cca                                               23


<210>  620
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  620
ugaccaauag ucuuagagua ucca                                              24


<210>  621
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  621
auccucuaug augggaga                                                     18


<210>  622
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  622
uauccucuau gaugggaga                                                    19


<210>  623
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  623
gguauccucu augaugggag a                                                 21


<210>  624
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  624
ugguauccuc uaugauggga ga                                                22


<210>  625
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  625
cugguauccu cuaugauggg aga                                               23


<210>  626
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  626
ccugguaucc ucuaugaugg gaga                                              24


<210>  627
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  627
uccugguauc cucuauga                                                     18


<210>  628
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  628
guccugguau ccucuauga                                                    19


<210>  629
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  629
aaguccuggu auccucuaug a                                                 21


<210>  630
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  630
gaaguccugg uauccucuau ga                                                22


<210>  631
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  631
agaaguccug guauccucua uga                                               23


<210>  632
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  632
aagaaguccu gguauccucu auga                                              24


<210>  633
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  633
ggagaaggaa acuagcua                                                     18


<210>  634
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  634
gggagaagga aacuagcua                                                    19


<210>  635
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  635
ugggagaagg aaacuagcua                                                   20


<210>  636
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  636
augggagaag gaaacuagcu a                                                 21


<210>  637
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  637
gaugggagaa ggaaacuagc ua                                                22


<210>  638
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  638
ugaugggaga aggaaacuag cua                                               23


<210>  639
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  639
augaugggag aaggaaacua gcua                                              24


<210>  640
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  640
aaagggaaga auaaauua                                                     18


<210>  641
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  641
uaaagggaag aauaaauua                                                    19


<210>  642
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  642
cuaaagggaa gaauaaauua                                                   20


<210>  643
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  643
gcuaaaggga agaauaaauu a                                                 21


<210>  644
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  644
agcuaaaggg aagaauaaau ua                                                22


<210>  645
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  645
uagcuaaagg gaagaauaaa uua                                               23


<210>  646
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  646
cuagcuaaag ggaagaauaa auua                                              24


<210>  647
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  647
agaguaucca gugaggcc                                                     18


<210>  648
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  648
uagaguaucc agugaggcc                                                    19


<210>  649
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  649
cuuagaguau ccagugaggc c                                                 21


<210>  650
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  650
ucuuagagua uccagugagg cc                                                22


<210>  651
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  651
gucuuagagu auccagugag gcc                                               23


<210>  652
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  652
agucuuagag uauccaguga ggcc                                              24


<210>  653
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  653
uagaguaucc agugaggc                                                     18


<210>  654
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  654
uuagaguauc cagugaggc                                                    19


<210>  655
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  655
cuuagaguau ccagugaggc                                                   20


<210>  656
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  656
ucuuagagua uccagugagg c                                                 21


<210>  657
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  657
gucuuagagu auccagugag gc                                                22


<210>  658
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  658
agucuuagag uauccaguga ggc                                               23


<210>  659
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  659
uagucuuaga guauccagug aggc                                              24


<210>  660
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  660
caggggccgg cggcuggc                                                     18


<210>  661
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  661
ccaggggccg gcggcuggc                                                    19


<210>  662
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  662
gccaggggcc ggcggcuggc                                                   20


<210>  663
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  663
ggccaggggc cggcggcugg c                                                 21


<210>  664
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  664
aggccagggg ccggcggcug gc                                                22


<210>  665
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  665
gaggccaggg gccggcggcu ggc                                               23


<210>  666
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  666
ugaggccagg ggccggcggc uggc                                              24


<210>  667
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  667
aaaauuagca guauccuc                                                     18


<210>  668
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  668
aaaaauuagc aguauccuc                                                    19


<210>  669
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  669
aaaaaauuag caguauccuc                                                   20


<210>  670
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  670
aaaaaaauua gcaguauccu c                                                 21


<210>  671
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  671
aaaaaaaauu agcaguaucc uc                                                22


<210>  672
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  672
uaaaaaaaau uagcaguauc cuc                                               23


<210>  673
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  673
auaaaaaaaa uuagcaguau ccuc                                              24


<210>  674
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  674
guuccacaca cucgcuuc                                                     18


<210>  675
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  675
aguuccacac acucgcuuc                                                    19


<210>  676
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  676
gcaguuccac acacucgcuu c                                                 21


<210>  677
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  677
agcaguucca cacacucgcu uc                                                22


<210>  678
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  678
cagcaguucc acacacucgc uuc                                               23


<210>  679
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  679
ucagcaguuc cacacacucg cuuc                                              24


<210>  680
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  680
uauccucuau gaugggag                                                     18


<210>  681
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  681
guauccucua ugaugggag                                                    19


<210>  682
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  682
gguauccucu augaugggag                                                   20


<210>  683
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  683
ugguauccuc uaugauggga g                                                 21


<210>  684
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  684
cugguauccu cuaugauggg ag                                                22


<210>  685
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  685
ccugguaucc ucuaugaugg gag                                               23


<210>  686
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  686
uccugguauc cucuaugaug ggag                                              24


<210>  687
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  687
gccggcggcu ggcuaggg                                                     18


<210>  688
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  688
ggccggcggc uggcuaggg                                                    19


<210>  689
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  689
gggccggcgg cuggcuaggg                                                   20


<210>  690
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  690
ggggccggcg gcuggcuagg g                                                 21


<210>  691
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  691
aggggccggc ggcuggcuag gg                                                22


<210>  692
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  692
caggggccgg cggcuggcua ggg                                               23


<210>  693
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  693
ccaggggccg gcggcuggcu aggg                                              24


<210>  694
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  694
ugguauccuc uaugaugg                                                     18


<210>  695
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  695
cugguauccu cuaugaugg                                                    19


<210>  696
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  696
ccugguaucc ucuaugaugg                                                   20


<210>  697
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  697
uccugguauc cucuaugaug g                                                 21


<210>  698
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  698
guccugguau ccucuaugau gg                                                22


<210>  699
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  699
aguccuggua uccucuauga ugg                                               23


<210>  700
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  700
aaguccuggu auccucuaug augg                                              24


<210>  701
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  701
guccugguau ccucuaug                                                     18


<210>  702
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  702
aguccuggua uccucuaug                                                    19


<210>  703
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  703
aaguccuggu auccucuaug                                                   20


<210>  704
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  704
gaaguccugg uauccucuau g                                                 21


<210>  705
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  705
agaaguccug guauccucua ug                                                22


<210>  706
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  706
aagaaguccu gguauccucu aug                                               23


<210>  707
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  707
aaagaagucc ugguauccuc uaug                                              24


<210>  708
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  708
cuaaagggaa gaauaaau                                                     18


<210>  709
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  709
gcuaaaggga agaauaaau                                                    19


<210>  710
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  710
agcuaaaggg aagaauaaau                                                   20


<210>  711
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  711
uagcuaaagg gaagaauaaa u                                                 21


<210>  712
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  712
cuagcuaaag ggaagaauaa au                                                22


<210>  713
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  713
acuagcuaaa gggaagaaua aau                                               23


<210>  714
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  714
aacuagcuaa agggaagaau aaau                                              24


<210>  715
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  715
aaacuggaau gacugaau                                                     18


<210>  716
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  716
aaaacuggaa ugacugaau                                                    19


<210>  717
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  717
gaaaaacugg aaugacugaa u                                                 21


<210>  718
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  718
agaaaaacug gaaugacuga au                                                22


<210>  719
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  719
gagaaaaacu ggaaugacug aau                                               23


<210>  720
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  720
agagaaaaac uggaaugacu gaau                                              24


<210>  721
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  721
ccugguaucc ucuaugau                                                     18


<210>  722
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  722
uccugguauc cucuaugau                                                    19


<210>  723
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  723
aguccuggua uccucuauga u                                                 21


<210>  724
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  724
aaguccuggu auccucuaug au                                                22


<210>  725
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  725
gaaguccugg uauccucuau gau                                               23


<210>  726
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  726
agaaguccug guauccucua ugau                                              24


<210>  727
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  727
aaauuagcag uauccucu                                                     18


<210>  728
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  728
aaaauuagca guauccucu                                                    19


<210>  729
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  729
aaaaaauuag caguauccuc u                                                 21


<210>  730
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  730
aaaaaaauua gcaguauccu cu                                                22


<210>  731
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  731
aaaaaaaauu agcaguaucc ucu                                               23


<210>  732
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  732
uaaaaaaaau uagcaguauc cucu                                              24


<210>  733
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  733
cacucgcuuc uggaacgu                                                     18


<210>  734
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  734
acacucgcuu cuggaacgu                                                    19


<210>  735
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  735
cacacucgcu ucuggaacgu                                                   20


<210>  736
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  736
acacacucgc uucuggaacg u                                                 21


<210>  737
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  737
cacacacucg cuucuggaac gu                                                22


<210>  738
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  738
ccacacacuc gcuucuggaa cgu                                               23


<210>  739
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  739
uccacacacu cgcuucugga acgu                                              24


<210>  740
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  740
cucaaugcaa auaucugu                                                     18


<210>  741
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  741
ucucaaugca aauaucugu                                                    19


<210>  742
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  742
aucucaaugc aaauaucugu                                                   20


<210>  743
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  743
uaucucaaug caaauaucug u                                                 21


<210>  744
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  744
cuaucucaau gcaaauaucu gu                                                22


<210>  745
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  745
acuaucucaa ugcaaauauc ugu                                               23


<210>  746
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  746
cacuaucuca augcaaauau cugu                                              24


<210>  747
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  747
aguuccacac acucgcuu                                                     18


<210>  748
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  748
caguuccaca cacucgcuu                                                    19


<210>  749
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  749
gcaguuccac acacucgcuu                                                   20


<210>  750
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  750
agcaguucca cacacucgcu u                                                 21


<210>  751
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  751
cagcaguucc acacacucgc uu                                                22


<210>  752
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  752
ucagcaguuc cacacacucg cuu                                               23


<210>  753
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  753
uucagcaguu ccacacacuc gcuu                                              24


<210>  754
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  754
aauuagcagu auccucuu                                                     18


<210>  755
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  755
aaauuagcag uauccucuu                                                    19


<210>  756
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  756
aaaaauuagc aguauccucu u                                                 21


<210>  757
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  757
aaaaaauuag caguauccuc uu                                                22


<210>  758
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  758
aaaaaaauua gcaguauccu cuu                                               23


<210>  759
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  759
aaaaaaaauu agcaguaucc ucuu                                              24


<210>  760
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  760
gaagaaaacu agcuaaa                                                      17


<210>  761
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  761
gcagcaguau ccucuug                                                      17


<210>  762
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  762
ggagaagaaa acuagcuaaa                                                   20


<210>  763
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  763
gggagaagaa aacuagcuaa                                                   20


<210>  764
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  764
guccugguau cuucuauggu                                                   20


<210>  765
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  765
agaagaaaac uagcuaa                                                      17


<210>  766
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  766
aguccuggua ucuucua                                                      17


<210>  767
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  767
ccaccauaga agauacc                                                      17


<210>  768
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  768
ccugguaucu ucuaugg                                                      17


<210>  769
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  769
cagcaguauc cucuugg                                                      17


<210>  770
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  770
aauuggaaug acugaau                                                      17


<210>  771
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  771
cugguaucuu cuauggu                                                      17


<210>  772
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  772
agcagcagua uccucuu                                                      17


<210>  773
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  773
agaaguccug guaucuucua                                                   20


<210>  774
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  774
cucccaccau agaagauacc                                                   20


<210>  775
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  775
aguccuggua ucuucuaugg                                                   20


<210>  776
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  776
aagcagcagu auccucuugg                                                   20


<210>  777
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  777
uaagcagcag uauccucuug                                                   20


<210>  778
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  778
agaauaaauu agagaaaaau                                                   20


<210>  779
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  779
aaaaauugga augacugaau                                                   20


<210>  780
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  780
auuaagcagc aguauccucu                                                   20


<210>  781
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  781
uuaagcagca guauccucuu                                                   20


<210>  782
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  782
auaaauuaga gaaaaau                                                      17


<210>  783
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  783
aagcagcagu auccucu                                                      17


<210>  784
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  784
gaagaaaacu agcuaaaggg                                                   20


<210>  785
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  785
gagaagaaaa cuagcuaaag gg                                                22


<210>  786
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  786
ggagaagaaa acuagcuaaa ggg                                               23


<210>  787
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  787
gggagaagaa aacuagcuaa aggg                                              24


<210>  788
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  788
gaauaaauua gagaaaaau                                                    19


<210>  789
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  789
gaagaauaaa uuagagaaaa au                                                22


<210>  790
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  790
ggaagaauaa auuagagaaa aau                                               23


<210>  791
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  791
gggaagaaua aauuagagaa aaau                                              24


<210>  792
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  792
agagaaaaau uggaauga                                                     18


<210>  793
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  793
uagagaaaaa uuggaauga                                                    19


<210>  794
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  794
uuagagaaaa auuggaauga                                                   20


<210>  795
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  795
auuagagaaa aauuggaaug a                                                 21


<210>  796
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  796
aauuagagaa aaauuggaau ga                                                22


<210>  797
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  797
aaauuagaga aaaauuggaa uga                                               23


<210>  798
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  798
uaaauuagag aaaaauugga auga                                              24


<210>  799
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  799
agaaaacuag cuaaaggg                                                     18


<210>  800
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  800
aagaaaacua gcuaaaggg                                                    19


<210>  801
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  801
agaagaaaac uagcuaaagg g                                                 21


<210>  802
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  802
aauaaauuag agaaaaau                                                     18


<210>  803
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  803
aagaauaaau uagagaaaaa u                                                 21


<210>  804
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  804
aguuuucuuc ucccacca                                                     18


<210>  805
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  805
uaguuuucuu cucccacca                                                    19


<210>  806
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  806
cuaguuuucu ucucccacca                                                   20


<210>  807
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  807
gcuaguuuuc uucucccacc a                                                 21


<210>  808
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  808
agcuaguuuu cuucucccac ca                                                22


<210>  809
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  809
uagcuaguuu ucuucuccca cca                                               23


<210>  810
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  810
uuagcuaguu uucuucuccc acca                                              24


<210>  811
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  811
ucccaccaua gaagauac                                                     18


<210>  812
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  812
cucccaccau agaagauac                                                    19


<210>  813
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  813
ucucccacca uagaagauac                                                   20


<210>  814
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  814
uucucccacc auagaagaua c                                                 21


<210>  815
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  815
cuucucccac cauagaagau ac                                                22


<210>  816
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  816
ucuucuccca ccauagaaga uac                                               23


<210>  817
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  817
uucuucuccc accauagaag auac                                              24


<210>  818
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  818
agaagaaaac uagcuaaa                                                     18


<210>  819
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  819
gagaagaaaa cuagcuaaa                                                    19


<210>  820
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  820
gggagaagaa aacuagcuaa a                                                 21


<210>  821
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  821
ugggagaaga aaacuagcua aa                                                22


<210>  822
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  822
gugggagaag aaaacuagcu aaa                                               23


<210>  823
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  823
ggugggagaa gaaaacuagc uaaa                                              24


<210>  824
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  824
aaaauuggaa ugacugaa                                                     18


<210>  825
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  825
aaaaauugga augacugaa                                                    19


<210>  826
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  826
gaaaaauugg aaugacugaa                                                   20


<210>  827
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  827
agaaaaauug gaaugacuga a                                                 21


<210>  828
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  828
gagaaaaauu ggaaugacug aa                                                22


<210>  829
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  829
agagaaaaau uggaaugacu gaa                                               23


<210>  830
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  830
uagagaaaaa uuggaaugac ugaa                                              24


<210>  831
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  831
gagaagaaaa cuagcuaa                                                     18


<210>  832
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  832
ggagaagaaa acuagcuaa                                                    19


<210>  833
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  833
ugggagaaga aaacuagcua a                                                 21


<210>  834
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  834
gugggagaag aaaacuagcu aa                                                22


<210>  835
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  835
ggugggagaa gaaaacuagc uaa                                               23


<210>  836
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  836
uggugggaga agaaaacuag cuaa                                              24


<210>  837
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  837
ggagaagaaa acuagcua                                                     18


<210>  838
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  838
gggagaagaa aacuagcua                                                    19


<210>  839
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  839
ugggagaaga aaacuagcua                                                   20


<210>  840
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  840
gugggagaag aaaacuagcu a                                                 21


<210>  841
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  841
ggugggagaa gaaaacuagc ua                                                22


<210>  842
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  842
uggugggaga agaaaacuag cua                                               23


<210>  843
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  843
auggugggag aagaaaacua gcua                                              24


<210>  844
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  844
uuaagcagca guauccuc                                                     18


<210>  845
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  845
auuaagcagc aguauccuc                                                    19


<210>  846
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  846
aauuaagcag caguauccuc                                                   20


<210>  847
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  847
aaauuaagca gcaguauccu c                                                 21


<210>  848
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  848
aaaauuaagc agcaguaucc uc                                                22


<210>  849
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  849
aaaaauuaag cagcaguauc cuc                                               23


<210>  850
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  850
aaaaaauuaa gcagcaguau ccuc                                              24


<210>  851
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  851
uaucuucuau ggugggag                                                     18


<210>  852
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  852
guaucuucua uggugggag                                                    19


<210>  853
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  853
gguaucuucu auggugggag                                                   20


<210>  854
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  854
ugguaucuuc uaugguggga g                                                 21


<210>  855
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  855
cugguaucuu cuaugguggg ag                                                22


<210>  856
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  856
ccugguaucu ucuauggugg gag                                               23


<210>  857
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  857
uccugguauc uucuauggug ggag                                              24


<210>  858
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  858
uccugguauc uucuaugg                                                     18


<210>  859
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  859
guccugguau cuucuaugg                                                    19


<210>  860
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  860
aaguccuggu aucuucuaug g                                                 21


<210>  861
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  861
gaaguccugg uaucuucuau gg                                                22


<210>  862
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  862
agaaguccug guaucuucua ugg                                               23


<210>  863
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  863
aagaaguccu gguaucuucu augg                                              24


<210>  864
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  864
ugguaucuuc uauggugg                                                     18


<210>  865
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  865
cugguaucuu cuauggugg                                                    19


<210>  866
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  866
ccugguaucu ucuauggugg                                                   20


<210>  867
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  867
uccugguauc uucuauggug g                                                 21


<210>  868
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  868
guccugguau cuucuauggu gg                                                22


<210>  869
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  869
aguccuggua ucuucuaugg ugg                                               23


<210>  870
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  870
aaguccuggu aucuucuaug gugg                                              24


<210>  871
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  871
guccugguau cuucuaug                                                     18


<210>  872
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  872
aguccuggua ucuucuaug                                                    19


<210>  873
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  873
aaguccuggu aucuucuaug                                                   20


<210>  874
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  874
gaaguccugg uaucuucuau g                                                 21


<210>  875
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  875
agaaguccug guaucuucua ug                                                22


<210>  876
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  876
aagaaguccu gguaucuucu aug                                               23


<210>  877
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  877
aaagaagucc ugguaucuuc uaug                                              24


<210>  878
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  878
aaauuggaau gacugaau                                                     18


<210>  879
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  879
aaaauuggaa ugacugaau                                                    19


<210>  880
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  880
gaaaaauugg aaugacugaa u                                                 21


<210>  881
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  881
agaaaaauug gaaugacuga au                                                22


<210>  882
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  882
gagaaaaauu ggaaugacug aau                                               23


<210>  883
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  883
agagaaaaau uggaaugacu gaau                                              24


<210>  884
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  884
uaagcagcag uauccucu                                                     18


<210>  885
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  885
uuaagcagca guauccucu                                                    19


<210>  886
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  886
aauuaagcag caguauccuc u                                                 21


<210>  887
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  887
aaauuaagca gcaguauccu cu                                                22


<210>  888
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  888
aaaauuaagc agcaguaucc ucu                                               23


<210>  889
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  889
aaaaauuaag cagcaguauc cucu                                              24


<210>  890
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  890
ccugguaucu ucuauggu                                                     18


<210>  891
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  891
uccugguauc uucuauggu                                                    19


<210>  892
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  892
aguccuggua ucuucuaugg u                                                 21


<210>  893
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  893
aaguccuggu aucuucuaug gu                                                22


<210>  894
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  894
gaaguccugg uaucuucuau ggu                                               23


<210>  895
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  895
agaaguccug guaucuucua uggu                                              24


<210>  896
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  896
aagcagcagu auccucuu                                                     18


<210>  897
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  897
uaagcagcag uauccucuu                                                    19


<210>  898
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  898
auuaagcagc aguauccucu u                                                 21


<210>  899
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  899
aauuaagcag caguauccuc uu                                                22


<210>  900
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  900
aaauuaagca gcaguauccu cuu                                               23


<210>  901
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Targeting domain

<400>  901
aaaauuaagc agcaguaucc ucuu                                              24


<210>  902
<211>  4758
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (2716)..(2719)
<223>  HPFH deletion site (4 bp del -225 to -222)

<220>
<221>  misc_feature
<222>  (2748)..(2753)
<223>  GATA1 binding motif

<220>
<221>  misc_feature
<222>  (2762)..(2767)
<223>  GATA1 binding motif

<220>
<221>  misc_feature
<222>  (2791)..(2799)
<223>  FKLF transcription factor binding motif

<220>
<221>  misc_feature
<222>  (2823)..(2830)
<223>  CP1/Coup TFII binding motif

<220>
<221>  misc_feature
<222>  (2824)..(2836)
<223>  HPFH deletion site (13 bp del -114 to -102)

<220>
<221>  misc_feature
<222>  (2991)..(2993)
<223>  Start codon

<400>  902
tttaggaagt caaggtttag gcagggatag ccattctatt ttattagggg caatactatt       60

tccaacggca tctggctttt ctcagccctt gtgaggctct acagggaggt tgaggtgtta      120

gagatcagag caggaaacag gtttttcttt ccacggtaac tacaatgaag tgatccttac      180

tttactaagg aacttttcat tttaagtgtt gacgcatgcc taaagaggtg aaattaatcc      240

cataccctta agtctacaga ctggtcacag catttcaagg aggagacctc attgtaagct      300

tctagggagg tggggactta ggtgaaggaa atgagccagc agaagctcac aagtcagcat      360

cagcgtgtca tgtctcagca gcagaacagc acggtcagat gaaaatatag tgtgaagaat      420

ttgtataaca ttaattgaga aggcagattc actggagttc ttatataatt gaaagttaat      480

gcacgttaat aagcaagagt ttagtttaat gtgatggtgt tatgaactta acgcttgtgt      540

ctccagaaaa ttcacatgct gaatccccaa ctcccaattg gctccatttg tgggggaggc      600

tttggaaaag taatcaggtt tagaggagct catgagagca gatccccatc atagaattat      660

tttcctcatc agaagcagag agattagcca tttctcttcc ttctggtgag gacacagtgg      720

gaagtcagcc acctgcaacc caggaagaga gccctgacca ggaaccagca gaaaagtgag      780

aaaaaatcct gttgttgaag tcacccagtc tatgctattt tgttatagca ccttgcacta      840

agtaaggcag atgaagaaag agaaaaaaat aagcttcggt gttcagtgga ttagaaacca      900

tgtttatctc aggtttacaa atctccactt gtcctctgtg tttcagaata aaataccaac      960

tctactactc tcatctgtaa gatgcaaata gtaagcctga gcccttctgt ctaactttga     1020

attctatttt ttcttcaacg tactttaggc ttgtaatgtg tttatataca gtgaaatgtc     1080

aagttctttc tttatatttc tttctttctt ttttttcctc agcctcagag ttttccacat     1140

gcccttccta ctttcaggaa cttctttctc caaacgtctt ctgcctggct ccatcaaatc     1200

ataaaggacc cacttcaaat gccatcactc actaccattt cacaattcgc actttctttc     1260

tttgtccttt ttttttttag taaaacaagt ttataaaaaa ttgaaggaat aaatgaatgg     1320

ctacttcata ggcagagtag acgcaagggc tactggttgc cgatttttat tgttattttt     1380

caatagtatg ctaaacaagg ggtagattat ttatgctgcc catttttaga ccataaaaga     1440

taacttcctg atgttgccat ggcatttttt tccttttaat tttatttcat ttcattttaa     1500

tttcgaaggt acatgtgcag gatgtgcagg cttgttacat gggtaaatgt gtgtctttct     1560

ggccttttag ccatctgtat caatgagcag atataagctt tacacaggat catgaaggat     1620

gaaagaattt caccaatatt ataataattt caatcaacct gatagcttag gggataaact     1680

aatttgaaga tacagcttgc ctccgataag ccagaattcc agagcttctg gcattataat     1740

ctagcaaggt tagagatcat ggatcacttt cagagaaaaa caaaaacaaa ctaaccaaaa     1800

gcaaaacaga accaaaaaac caccataaat acttcctacc ctgttaatgg tccaatatgt     1860

cagaaacagc actgtgttag aaataaagct gtctaaagta cactaatatt cgagttataa     1920

tagtgtgtgg actattagtc aataaaaaca acccttgcct ctttagagtt gttttccatg     1980

tacacgcaca tcttatgtct tagagtaaga ttccctgaga agtgaaccta gcatttatac     2040

aagataatta attctaatcc acagtacctg ccaaagaaca ttctaccatc atctttactg     2100

agcatagaag agctacgcca aaaccctggg tcatcagcca gcacacacac ttatccagtg     2160

gtaaatacac atcatctggt gtatacatac atacctgaat atggaatcaa atatttttct     2220

aagatgaaac agtcatgatt tatttcaaat aggtacggat aagtagatat tgaggtaagc     2280

attaggtctt atattatgta acactaatct attactgcgc tgaaactgtg gctttataga     2340

aattgttttc actgcactat tgagaaatta agagataatg gcaaaagtca caaagagtat     2400

attcaaaaag aagtatagca ctttttcctt agaaaccact gctaactgaa agagactaag     2460

atttgtcccg tcaaaaatcc tggacctatg cctaaaacac atttcacaat ccctgaactt     2520

ttcaaaaatt ggtacatgct ttagctttaa actacaggcc tcactggagc tagagacaag     2580

aaggtaaaaa acggctgaca aaagaagtcc tggtatcctc tatgatggga gaaggaaact     2640

agctaaaggg aagaataaat tagagaaaaa ctggaatgac tgaatcggaa caaggcaaag     2700

gctataaaaa aaattagcag tatcctcttg ggggcccctt ccccacacta tctcaatgca     2760

aatatctgtc tgaaacggtc cctggctaaa ctccacccat gggttggcca gccttgcctt     2820

gaccaatagc cttgacaagg caaacttgac caatagtctt agagtatcca gtgaggccag     2880

gggccggcgg ctggctaggg atgaagaata aaaggaagca cccttcagca gttccacaca     2940

ctcgcttctg gaacgtctga ggttatcaat aagctcctag tccagacgcc atgggtcatt     3000

tcacagagga ggacaaggct actatcacaa gcctgtgggg caaggtgaat gtggaagatg     3060

ctggaggaga aaccctggga aggtaggctc tggtgaccag gacaagggag ggaaggaagg     3120

accctgtgcc tggcaaaagt ccaggtcgct tctcaggatt tgtggcacct tctgactgtc     3180

aaactgttct tgtcaatctc acaggctcct ggttgtctac ccatggaccc agaggttctt     3240

tgacagcttt ggcaacctgt cctctgcctc tgccatcatg ggcaacccca aagtcaaggc     3300

acatggcaag aaggtgctga cttccttggg agatgccaca aagcacctgg atgatctcaa     3360

gggcaccttt gcccagctga gtgaactgca ctgtgacaag ctgcatgtgg atcctgagaa     3420

cttcaaggtg agtccaggag atgtttcagc cctgttgcct ttagtctcga ggcaacttag     3480

acaacggagt attgatctga gcacagcagg gtgtgagctg tttgaagata ctggggttgg     3540

gggtgaagaa actgcagagg actaactggg ctgagaccca gtggtaatgt tttagggcct     3600

aaggagtgcc tctaaaaatc tagatggaca attttgactt tgagaaaaga gaggtggaaa     3660

tgaggaaaat gacttttctt tattagattc cagtagaaag aactttcatc tttccctcat     3720

ttttgttgtt ttaaaacatc tatctggagg caggacaagt atggtcgtta aaaagatgca     3780

ggcagaaggc atatattggc tcagtcaaag tggggaactt tggtggccaa acatacattg     3840

ctaaggctat tcctatatca gctggacaca tataaaatgc tgctaatgct tcattacaaa     3900

cttatatcct ttaattccag atgggggcaa agtatgtcca ggggtgagga acaattgaaa     3960

catttgggct ggagtagatt ttgaaagtca gctctgtgtg tgtgtgtgtg tgtgcgcgcg     4020

cgcgtgtgtg tgtgtgtgtc agcgtgtgtt tcttttaacg tcttcagcct acaacataca     4080

gggttcatgg tggcaagaag atagcaagat ttaaattatg gccagtgact agtgcttgaa     4140

ggggaacaac tacctgcatt taatgggaag gcaaaatctc aggctttgag ggaagttaac     4200

ataggcttga ttctgggtgg aagcttggtg tgtagttatc tggaggccag gctggagctc     4260

tcagctcact atgggttcat ctttattgtc tcctttcatc tcaacagctc ctgggaaatg     4320

tgctggtgac cgttttggca atccatttcg gcaaagaatt cacccctgag gtgcaggctt     4380

cctggcagaa gatggtgact gcagtggcca gtgccctgtc ctccagatac cactgagctc     4440

actgcccatg attcagagct ttcaaggata ggctttattc tgcaagcaat acaaataata     4500

aatctattct gctgagagat cacacatgat tttcttcagc tctttttttt acatcttttt     4560

aaatatatga gccacaaagg gtttatattg agggaagtgt gtatgtgtat ttctgcatgc     4620

ctgtttgtgt ttgtggtgtg tgcatgctcc tcatttattt ttatatgaga tgtgcatttt     4680

gatgagcaaa taaaagcagt aaagacactt gtacacggga gttctgcaag tgggagtaaa     4740

tggtgtagga gaaatccg                                                   4758


<210>  903
<211>  4773
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1233)..(1238)
<223>  GATA1 binding site

<220>
<221>  misc_feature
<222>  (2672)..(2677)
<223>  GATA1 binding site

<220>
<221>  misc_feature
<222>  (2686)..(2691)
<223>  GATA1 binding site

<220>
<221>  misc_feature
<222>  (2715)..(2723)
<223>  FKLF transcription factor binding motif

<220>
<221>  misc_feature
<222>  (2747)..(2754)
<223>  CP1/Coup TFII binding motif

<220>
<221>  misc_feature
<222>  (2748)..(2760)
<223>  HPFH deletion site (13 bp del -114 to -102)

<220>
<221>  misc_feature
<222>  (2915)..(2917)
<223>  Start codon

<400>  903
ttatgtcatt accagagtta aaattctata atggcttctc actccctacc actgaggaca       60

agtttatgtc cttaggttta tgcttccctg aaacaatacc acctgctatt ctccacttta      120

catatcaacg gcactggttc tttatctaac tctctggcac agcaggagtt tgttttcttc      180

tgcttcagag ctttgaattt actatttcag cttctaaact ttatttggca atgccttccc      240

atggcagatt ccttctgtca ttttgcctct gttcgaatac tttctcctta atttcattct      300

tagttaataa tatctgaaat tattttgttg tttaacttaa ttattaattt tatgtatgtt      360

ctacctagat tataatcttc agaggaaagt tttattctct gacttattta acttaaatgc      420

ccactacttt aaaaattatg acatttattt aacagatatt tgctgaacaa atgtttgaaa      480

atacatggga aagaatgctt gaaaacactt gaaattgctt gtgtaaagaa acagttttat      540

cagttaggat ttaatcaatg tcagaagcaa tgatatagga aaaatcgagg aataagacag      600

ttatggataa ggagaaatca acaaactctt aaaagatatt gcctcaaaag cataagagga      660

aataagggtt tatacatgac ttttagaaca ctgccttggt ttttggataa atggggaagt      720

tgtttgaaaa caggagggat cctagatatt ccttagtctg aggaggagca attaagattc      780

acttgtttag aggctgggag tggtggctca cgcctgtaat cccagaattt tgggaggcca      840

aggcaggcag atcacctgag gtcaagagtt caagaccaac ctggccaaca tggtgaaatc      900

ccatctctac aaaaatacaa aaattagaca ggcatgatgg caagtgcctg taatcccagc      960

tacttgggag gctgaggaag gagaattgct tgaacctgga aggcaggagt tgcagtgagc     1020

cgagatcata ccactgcact ccagcctggg tgacagaaca agactctgtc tcaaaaaaaa     1080

aaaagagaga ttcaaaagat tcacttgttt aggccttagc gggcttagac accagtctct     1140

gacacattct taaaggtcag gctctacaaa tggaacccaa ccagactctc agatatggcc     1200

aaagatctat acacacccat ctcacagatc ccctatctta aagagaccct aatttgggtt     1260

cacctcagtc tctataatct gtaccagcat accaataaaa atctttctca cccatcctta     1320

gattgagaga agtcacttat tattatgtga gtaactggaa gatactgata agttgacaaa     1380

tctttttctt tcctttctta ttcaactttt attttaactt ccaaagaaca agtgcaatat     1440

gtgcagcttt gttgcgcagg tcaacatgta tctttctggt cttttagccg cctaacactt     1500

tgagcagata taagccttac acaggattat gaagtctgaa aggattccac caatattatt     1560

ataattccta tcaacctgat aggttagggg aaggtagagc tctcctccaa taagccagat     1620

ttccagagtt tctgacgtca taatctacca aggtcatgga tcgagttcag agaaaaaaca     1680

aaagcaaaac caaacctacc aaaaaataaa aatcccaaag aaaaaataaa gaaaaaaaca     1740

gcatgaatac ttcctgccat gttaagtggc caatatgtca gaaacagcac tgagttacag     1800

ataaagatgt ctaaactaca gtgacatccc agctgtcaca gtgtgtggac tattagtcaa     1860

taaaacagtc cctgcctctt aagagttgtt ttccatgcaa atacatgtct tatgtcttag     1920

aataagattc cctaagaagt gaacctagca tttatacaag ataattaatt ctaatccata     1980

gtatctggta aagagcattc taccatcatc tttaccgagc atagaagagc tacaccaaaa     2040

ccctgggtca tcagccagca catacactta tccagtgata aatacacatc atcgggtgcc     2100

tacatacata cctgaatata aaaaaaatac ttttgctgag atgaaacagg cgtgatttat     2160

ttcaaatagg tacggataag tagatattga agtaaggatt cagtcttata ttatattaca     2220

taacattaat ctattcctgc actgaaactg ttgctttata ggatttttca ctacactaat     2280

gagaacttaa gagataatgg cctaaaacca cagagagtat attcaaagat aagtatagca     2340

cttcttattt ggaaaccaat gcttactaaa tgagactaag acgtgtccca tcaaaaatcc     2400

tggacctatg cctaaaacac atttcacaat ccctgaactt ttcaaaaatt ggtacatgct     2460

ttaactttaa actacaggcc tcactggagc tacagacaag aaggtgaaaa acggctgaca     2520

aaagaagtcc tggtatcttc tatggtggga gaagaaaact agctaaaggg aagaataaat     2580

tagagaaaaa ttggaatgac tgaatcggaa caaggcaaag gctataaaaa aaattaagca     2640

gcagtatcct cttgggggcc ccttccccac actatctcaa tgcaaatatc tgtctgaaac     2700

ggtccctggc taaactccac ccatgggttg gccagccttg ccttgaccaa tagccttgac     2760

aaggcaaact tgaccaatag tcttagagta tccagtgagg ccaggggccg gcggctggct     2820

agggatgaag aataaaagga agcacccttc agcagttcca cacactcgct tctggaacgt     2880

ctgaggttat caataagctc ctagtccaga cgccatgggt catttcacag aggaggacaa     2940

ggctactatc acaagcctgt ggggcaaggt gaatgtggaa gatgctggag gagaaaccct     3000

gggaaggtag gctctggtga ccaggacaag ggagggaagg aaggaccctg tgcctggcaa     3060

aagtccaggt cgcttctcag gatttgtggc accttctgac tgtcaaactg ttcttgtcaa     3120

tctcacaggc tcctggttgt ctacccatgg acccagaggt tctttgacag ctttggcaac     3180

ctgtcctctg cctctgccat catgggcaac cccaaagtca aggcacatgg caagaaggtg     3240

ctgacttcct tgggagatgc cataaagcac ctggatgatc tcaagggcac ctttgcccag     3300

ctgagtgaac tgcactgtga caagctgcat gtggatcctg agaacttcaa ggtgagtcca     3360

ggagatgttt cagcactgtt gcctttagtc tcgaggcaac ttagacaact gagtattgat     3420

ctgagcacag cagggtgtga gctgtttgaa gatactgggg ttgggagtga agaaactgca     3480

gaggactaac tgggctgaga cccagtggca atgttttagg gcctaaggag tgcctctgaa     3540

aatctagatg gacaactttg actttgagaa aagagaggtg gaaatgagga aaatgacttt     3600

tctttattag atttcggtag aaagaacttt cacctttccc ctatttttgt tattcgtttt     3660

aaaacatcta tctggaggca ggacaagtat ggtcattaaa aagatgcagg cagaaggcat     3720

atattggctc agtcaaagtg gggaactttg gtggccaaac atacattgct aaggctattc     3780

ctatatcagc tggacacata taaaatgctg ctaatgcttc attacaaact tatatccttt     3840

aattccagat gggggcaaag tatgtccagg ggtgaggaac aattgaaaca tttgggctgg     3900

agtagatttt gaaagtcagc tctgtgtgtg tgtgtgtgtg tgtgcgcgcg tgtgtttgtg     3960

tgtgtgtgag agcgtgtgtt tcttttaacg ttttcagcct acagcataca gggttcatgg     4020

tggcaagaag ataacaagat ttaaattatg gccagtgact agtgctgcaa gaagaacaac     4080

tacctgcatt taatgggaaa gcaaaatctc aggctttgag ggaagttaac ataggcttga     4140

ttctgggtgg aagcttggtg tgtagttatc tggaggccag gctggagctc tcagctcact     4200

atgggttcat ctttattgtc tcctttcatc tcaacagctc ctgggaaatg tgctggtgac     4260

cgttttggca atccatttcg gcaaagaatt cacccctgag gtgcaggctt cctggcagaa     4320

gatggtgact ggagtggcca gtgccctgtc ctccagatac cactgagctc actgcccatg     4380

atgcagagct ttcaaggata ggctttattc tgcaagcaat caaataataa atctattctg     4440

ctaagagatc acacatggtt gtcttcagtt ctttttttat gtctttttaa atatatgagc     4500

cacaaagggt tttatgttga gggatgtgtt tatgtgtatt tatacatggc tatgtgtgtt     4560

tgtgtcatgt gcacactcca cacttttttg tttacgttag atgtgggttt tgatgagcaa     4620

ataaaagaac taggcaataa agaaacttgt acatgggagt tctgcaagtg ggagtaaaag     4680

gtgcaggaga aatctggttg gaagaaagac ctctatagga caggactcct cagaaacaga     4740

tgttttggaa gagatgggga aaggttcagt gaa                                  4773


<210>  904
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssODN1 5' homology arm

<400>  904
gggtgcttcc ttttattctt catccctagc cagccgccgg cccctggcct cactggatac       60

tctaagacta ttggtcaagt ttgcctt                                           87


<210>  905
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssODN1 3' homology arm

<400>  905
gtcaaggcaa ggctggccaa cccatgggtg gagtttagcc agggaccgtt tcagacagat       60

atttgcattg agatagtgtg gggaagggg                                         89


<210>  906
<211>  176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssODN1

<400>  906
gggtgcttcc ttttattctt catccctagc cagccgccgg cccctggcct cactggatac       60

tctaagacta ttggtcaagt ttgccttgtc aaggcaaggc tggccaaccc atgggtggag      120

tttagccagg gaccgtttca gacagatatt tgcattgaga tagtgtgggg aagggg          176


<210>  907
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PhTx ssODN1 5' homology arm


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<400>  907
gggtgcttcc ttttattctt catccctagc cagccgccgg cccctggcct cactggatac       60

tctaagacta ttggtcaagt ttgcctt                                           87


<210>  908
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PhTx ssODN1 3' homology arm


<220>
<221>  misc_feature
<222>  (88)..(89)
<223>  Linked by phosphorothioate (PS) bond

<400>  908
gtcaaggcaa ggctggccaa cccatgggtg gagtttagcc agggaccgtt tcagacagat       60

atttgcattg agatagtgtg gggaagggg                                         89


<210>  909
<211>  176
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PhTx ssODN1


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (175)..(176)
<223>  Linked by phosphorothioate (PS) bond

<400>  909
gggtgcttcc ttttattctt catccctagc cagccgccgg cccctggcct cactggatac       60

tctaagacta ttggtcaagt ttgccttgtc aaggcaaggc tggccaaccc atgggtggag      120

tttagccagg gaccgtttca gacagatatt tgcattgaga tagtgtgggg aagggg          176


<210>  910
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  910
ggctattggt caaggca                                                      17


<210>  911
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  911
caaggctatt ggtcaaggca                                                   20


<210>  912
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  912
tgccttgtca aggctat                                                      17


<210>  913
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  913
gtttgccttg tcaaggctat                                                   20


<210>  914
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  914
gaccaatagc cttgaca                                                      17


<210>  915
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  915
cttgaccaat agccttgaca                                                   20


<210>  916
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  916
gtcaaggcta ttggtca                                                      17


<210>  917
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  917
cttgtcaagg ctattggtca                                                   20


<210>  918
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  918
tcaagtttgc cttgtca                                                      17


<210>  919
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  919
tggtcaagtt tgccttgtca                                                   20


<210>  920
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  920
ggcuauuggu caaggcaagg                                                   20


<210>  921
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  921
caaggcuauu ggucaaggca agg                                               23


<210>  922
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  922
ugccuuguca aggcuauugg                                                   20


<210>  923
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  923
guuugccuug ucaaggcuau ugg                                               23


<210>  924
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  924
gaccaauagc cuugacaagg                                                   20


<210>  925
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  925
cuugaccaau agccuugaca agg                                               23


<210>  926
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  926
gucaaggcua uuggucaagg                                                   20


<210>  927
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  927
cuugucaagg cuauugguca agg                                               23


<210>  928
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  928
ucaaguuugc cuugucaagg                                                   20


<210>  929
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  929
uggucaaguu ugccuuguca agg                                               23


<210>  930
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  930
ggctattggt caaggcaagg                                                   20


<210>  931
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  931
caaggctatt ggtcaaggca agg                                               23


<210>  932
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  932
tgccttgtca aggctattgg                                                   20


<210>  933
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  933
gtttgccttg tcaaggctat tgg                                               23


<210>  934
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  934
gaccaatagc cttgacaagg                                                   20


<210>  935
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  935
cttgaccaat agccttgaca agg                                               23


<210>  936
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  936
gtcaaggcta ttggtcaagg                                                   20


<210>  937
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  937
cttgtcaagg ctattggtca agg                                               23


<210>  938
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  938
tcaagtttgc cttgtcaagg                                                   20


<210>  939
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  939
tggtcaagtt tgccttgtca agg                                               23


<210>  940
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  940
aguauccagu gaggcca                                                      17


<210>  941
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  941
ggcaaggcug gccaacccau                                                   20


<210>  942
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  942
uauuugcauu gagauagugu                                                   20


<210>  943
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  943
agtatccagt gaggcca                                                      17


<210>  944
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  944
ggcaaggctg gccaacccat                                                   20


<210>  945
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  945
tatttgcatt gagatagtgt                                                   20


<210>  946
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  946
aguauccagu gaggccaggg                                                   20


<210>  947
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  947
ggcaaggcug gccaacccau ggg                                               23


<210>  948
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  948
uauuugcauu gagauagugu ggg                                               23


<210>  949
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  949
agtatccagt gaggccaggg                                                   20


<210>  950
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  950
ggcaaggctg gccaacccat ggg                                               23


<210>  951
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  951
tatttgcatt gagatagtgt ggg                                               23


<210>  952
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  952
cuaacaguug cuuuuaucac                                                   20


<210>  953
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  953
gggcgugggu gggguagaag                                                   20


<210>  954
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  954
cucuuagaca uaacacacca                                                   20


<210>  955
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  955
aucagaggcc aaacccuucc                                                   20


<210>  956
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  956
ctaacagttg cttttatcac                                                   20


<210>  957
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  957
gggcgtgggt ggggtagaag                                                   20


<210>  958
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  958
ctcttagaca taacacacca                                                   20


<210>  959
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain

<400>  959
atcagaggcc aaacccttcc                                                   20


<210>  960
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  960
cuaacaguug cuuuuaucac agg                                               23


<210>  961
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  961
gggcgugggu gggguagaag agg                                               23


<210>  962
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  962
cucuuagaca uaacacacca ggg                                               23


<210>  963
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  963
aucagaggcc aaacccuucc ugg                                               23


<210>  964
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  964
ctaacagttg cttttatcac agg                                               23


<210>  965
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  965
gggcgtgggt ggggtagaag agg                                               23


<210>  966
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  966
ctcttagaca taacacacca ggg                                               23


<210>  967
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeting domain plus PAM (NGG)

<400>  967
atcagaggcc aaacccttcc tgg                                               23


<210>  968
<211>  115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +58 DHS enhancer

<400>  968
caactttgaa gctagtctag tgcaagctaa cagttgcttt tatcacaggc tccaggaagg       60

gtttggcctc tgattagggt gggggcgtgg gtggggtaga agaggactgg cagac           115


<210>  969
<211>  801
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +58 DHS enhancer region including ~500 bp upstream and ~200 bp 
       downstream

<400>  969
aggtaccttt tgtgtgtatg tgctgattga gggcccattg agaatatttt gacttttagg       60

gaagctccaa actctcaaac cacagggatc acaacacata cgtgtgtctg ttatgacgtt      120

atatgtaagc atcacaacag gcagagaatg tctgcacccc accctggaaa acagcctgac      180

tgtgccccat gggcaaacca gactagttta tagggggttc tactctgagg tactgatgga      240

ccttgggtgc tattcctgtg ataaggaagg cagctagaca ggacttggga gttatctgta      300

gtgagatggc tgaaaagcga tacagggctg gctctatgcc ccaggtgtgc ataagtaaga      360

gcagatagct gattccagtg caaagtccat acaggtaata acataggcca gaaaagagat      420

atggcatcta ctcttagaca taacacacca gggtcaatac aactttgaag ctagtctagt      480

gcaagctaac agttgctttt atcacaggct ccaggaaggg tttggcctct gattagggtg      540

ggggcgtggg tggggtagaa gaggactggc agacctctcc atcggtggcc gtttgcccag      600

gggggcctct ttcggaaggc tctcttggtg atggagaatt ggattttatt tctcaatggg      660

aatgaaataa tttgtatgcc atgccgtgtg gactcccaaa attgtaaagg aggtgaagct      720

tcccctgtct gcactctccc ctcctcataa ttgtccattt ttcatctgtc gggctgtcca      780

cccatccatc acatataggc a                                                801


<210>  970
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Unmodified Sp35 gRNA (OLI7066)

<400>  970
cuugucaagg cuauugguca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  971
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Sp35 gRNA w/ 5' and 3' PS/OMe (OLI8394)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (97)..(98)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (98)..(99)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (98)..(98)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(99)
<223>  Modified to contain 2'-O-methyl

<400>  971
cuugucaagg cuauugguca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  972
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Unmodified Sp35 gRNA (OLI7066)

<400>  972
cttgtcaagg ctattggtca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt                            100


<210>  973
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sp35 gRNA w/ 5' and 3' PS/OMe (OLI8394)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (97)..(98)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (98)..(98)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (98)..(99)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(99)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<400>  973
cttgtcaagg ctattggtca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt                            100


<210>  974
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 18nt - positive strand (OLI16409)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  974
ggtgcttcct tttattcttc atccctagcc agccgccggc ccctggcctc actggatact       60

ctaagactat tggtcaagtt tgccttgtca aggctggcca acccatgggt ggagtttagc      120

cagggaccgt ttcagacaga tatttgcatt gagatagtgt ggggaagggg cccccaagag      180


<210>  975
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 18nt - negative strand (OLI16410)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  975
ctcttggggg ccccttcccc acactatctc aatgcaaata tctgtctgaa acggtccctg       60

gctaaactcc acccatgggt tggccagcct tgacaaggca aacttgacca atagtcttag      120

agtatccagt gaggccaggg gccggcggct ggctagggat gaagaataaa aggaagcacc      180


<210>  976
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 11nt - negative strand (OLI16411)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  976
ggccccttcc ccacactatc tcaatgcaaa tatctgtctg aaacggtccc tggctaaact       60

ccacccatgg gttggccagc cttgccttga gacaaggcaa acttgaccaa tagtcttaga      120

gtatccagtg aggccagggg ccggcggctg gctagggatg aagaataaaa ggaagcaccc      180


<210>  977
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 13nt - negative strand (OLI16412)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  977
ggccccttcc ccacactatc tcaatgcaaa tatctgtctg aaacggtccc tggctaaact       60

ccacccatgg gttggccagc cttgccttga caaggcaaac ttgaccaata gtcttagagt      120

atccagtgag gccaggggcc ggcggctggc tagggatgaa gaataaaagg aagcaccctt      180


<210>  978
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 11nt - positive strand (OLI16413)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  978
gggtgcttcc ttttattctt catccctagc cagccgccgg cccctggcct cactggatac       60

tctaagacta ttggtcaagt ttgccttgtc tcaaggcaag gctggccaac ccatgggtgg      120

agtttagcca gggaccgttt cagacagata tttgcattga gatagtgtgg ggaaggggcc      180


<210>  979
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 13nt - positive strand (OLI16414)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  979
aagggtgctt ccttttattc ttcatcccta gccagccgcc ggcccctggc ctcactggat       60

actctaagac tattggtcaa gtttgccttg tcaaggcaag gctggccaac ccatgggtgg      120

agtttagcca gggaccgttt cagacagata tttgcattga gatagtgtgg ggaaggggcc      180


<210>  980
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 117G>A - negative strand (OLI16415)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  980
ggccccttcc ccacactatc tcaatgcaaa tatctgtctg aaacggtccc tggctaaact       60

ccacccatgg gttggccagc cttgccttaa ccaatagcct tgacaaggca aacttgacca      120

atagtcttag agtatccagt gaggccaggg gccggcggct ggctagggat gaagaataaa      180


<210>  981
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 117G>A - positive strand (OLI16416)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  981
tttattcttc atccctagcc agccgccggc ccctggcctc actggatact ctaagactat       60

tggtcaagtt tgccttgtca aggctattgg ttaaggcaag gctggccaac ccatgggtgg      120

agtttagcca gggaccgttt cagacagata tttgcattga gatagtgtgg ggaaggggcc      180


<210>  982
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 1nt - negative strand (OLI16417)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  982
ggccccttcc ccacactatc tcaatgcaaa tatctgtctg aaacggtccc tggctaaact       60

ccacccatgg gttggccagc cttgccttgc caatagcctt gacaaggcaa acttgaccaa      120

tagtcttaga gtatccagtg aggccagggg ccggcggctg gctagggatg aagaataaaa      180


<210>  983
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 1nt - positive strand (OLI16418)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  983
ttttattctt catccctagc cagccgccgg cccctggcct cactggatac tctaagacta       60

ttggtcaagt ttgccttgtc aaggctattg gcaaggcaag gctggccaac ccatgggtgg      120

agtttagcca gggaccgttt cagacagata tttgcattga gatagtgtgg ggaaggggcc      180


<210>  984
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt 40/80 - negative strand (OLI16419)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (119)..(120)
<223>  Linked by phosphorothioate (PS) bond

<400>  984
tggctaaact ccacccatgg gttggccagc cttgccttga tagccttgac aaggcaaact       60

tgaccaatag tcttagagta tccagtgagg ccaggggccg gcggctggct agggatgaag      120


<210>  985
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt 30/70 - negative strand (OLI16420)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<400>  985
ccacccatgg gttggccagc cttgccttga tagccttgac aaggcaaact tgaccaatag       60

tcttagagta tccagtgagg ccaggggccg gcggctggct                            100


<210>  986
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt - negative strand (OLI16421)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<400>  986
aaacggtccc tggctaaact ccacccatgg gttggccagc cttgccttga tagccttgac       60

aaggcaaact tgaccaatag tcttagagta tccagtgagg                            100


<210>  987
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt - negative strand (OLI16422)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (119)..(120)
<223>  Linked by phosphorothioate (PS) bond

<400>  987
tatctgtctg aaacggtccc tggctaaact ccacccatgg gttggccagc cttgccttga       60

tagccttgac aaggcaaact tgaccaatag tcttagagta tccagtgagg ccaggggccg      120


<210>  988
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt - negative strand (OLI16423)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (139)..(140)
<223>  Linked by phosphorothioate (PS) bond

<400>  988
tcaatgcaaa tatctgtctg aaacggtccc tggctaaact ccacccatgg gttggccagc       60

cttgccttga tagccttgac aaggcaaact tgaccaatag tcttagagta tccagtgagg      120

ccaggggccg gcggctggct                                                  140


<210>  989
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt - negative strand (OLI16424)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  989
ggccccttcc ccacactatc tcaatgcaaa tatctgtctg aaacggtccc tggctaaact       60

ccacccatgg gttggccagc cttgccttga tagccttgac aaggcaaact tgaccaatag      120

tcttagagta tccagtgagg ccaggggccg gcggctggct agggatgaag aataaaagga      180


<210>  990
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt 40/80 - positive strand (OLI16425)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (119)..(120)
<223>  Linked by phosphorothioate (PS) bond

<400>  990
tactctaaga ctattggtca agtttgcctt gtcaaggcta tcaaggcaag gctggccaac       60

ccatgggtgg agtttagcca gggaccgttt cagacagata tttgcattga gatagtgtgg      120


<210>  991
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt 30/70 - positive strand (OLI16426)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<400>  991
ctattggtca agtttgcctt gtcaaggcta tcaaggcaag gctggccaac ccatgggtgg       60

agtttagcca gggaccgttt cagacagata tttgcattga                            100


<210>  992
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt - positive strand (OLI16427)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<400>  992
cctcactgga tactctaaga ctattggtca agtttgcctt gtcaaggcta tcaaggcaag       60

gctggccaac ccatgggtgg agtttagcca gggaccgttt                            100


<210>  993
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt - positive strand (OLI16428)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (119)..(120)
<223>  Linked by phosphorothioate (PS) bond

<400>  993
cggcccctgg cctcactgga tactctaaga ctattggtca agtttgcctt gtcaaggcta       60

tcaaggcaag gctggccaac ccatgggtgg agtttagcca gggaccgttt cagacagata      120


<210>  994
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt - positive strand (OLI16429)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (139)..(140)
<223>  Linked by phosphorothioate (PS) bond

<400>  994
agccagccgc cggcccctgg cctcactgga tactctaaga ctattggtca agtttgcctt       60

gtcaaggcta tcaaggcaag gctggccaac ccatgggtgg agtttagcca gggaccgttt      120

cagacagata tttgcattga                                                  140


<210>  995
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 4nt - positive strand (OLI16430)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (179)..(180)
<223>  Linked by phosphorothioate (PS) bond

<400>  995
tccttttatt cttcatccct agccagccgc cggcccctgg cctcactgga tactctaaga       60

ctattggtca agtttgcctt gtcaaggcta tcaaggcaag gctggccaac ccatgggtgg      120

agtttagcca gggaccgttt cagacagata tttgcattga gatagtgtgg ggaaggggcc      180


<210>  996
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Sp37 gRNA (OLI17075)

<400>  996
cuugaccaau agccuugaca guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  997
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SpA gRNA (OLI17074)

<400>  997
ggcaaggcug gccaacccau guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  998
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sp37 gRNA (OLI17075)

<400>  998
cttgaccaat agccttgaca gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt                            100


<210>  999
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpA gRNA (OLI17074)

<400>  999
ggcaaggctg gccaacccat gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt                            100


<210>  1000
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-nNLS

<400>  1000

Met Lys His His His His His His Met Thr Gln Phe Glu Gly Phe Thr 
1               5                   10                  15      


Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln 
            20                  25                  30          


Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp 
        35                  40                  45              


Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg 
    50                  55                  60                  


Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu Asp 
65                  70                  75                  80  


Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr 
                85                  90                  95      


Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn 
            100                 105                 110         


Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala 
        115                 120                 125             


Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu 
    130                 135                 140                 


Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr Thr 
145                 150                 155                 160 


Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr 
                165                 170                 175     


Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu Asp 
            180                 185                 190         


Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro Lys 
        195                 200                 205             


Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val Pro 
    210                 215                 220                 


Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile Phe 
225                 230                 235                 240 


Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln 
                245                 250                 255     


Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly 
            260                 265                 270         


Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu Val 
        275                 280                 285             


Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile Ala 
    290                 295                 300                 


Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp 
305                 310                 315                 320 


Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu 
                325                 330                 335     


Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn 
            340                 345                 350         


Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp 
        355                 360                 365             


Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser Ser 
    370                 375                 380                 


Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg 
385                 390                 395                 400 


Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys 
                405                 410                 415     


Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile Ile 
            420                 425                 430         


Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser 
        435                 440                 445             


Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro Thr 
    450                 455                 460                 


Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp 
465                 470                 475                 480 


Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp Glu 
                485                 490                 495     


Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys 
            500                 505                 510         


Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr 
        515                 520                 525             


Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe Gln 
    530                 535                 540                 


Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu Lys Asn Asn 
545                 550                 555                 560 


Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met 
                565                 570                 575     


Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu 
            580                 585                 590         


Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp 
        595                 600                 605             


Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala Val Thr 
    610                 615                 620                 


Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe 
625                 630                 635                 640 


Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro 
                645                 650                 655     


Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly 
            660                 665                 670         


Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe Thr 
        675                 680                 685             


Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser 
    690                 695                 700                 


Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala 
705                 710                 715                 720 


Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala Glu 
                725                 730                 735     


Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln 
            740                 745                 750         


Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn Leu 
        755                 760                 765             


His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys 
    770                 775                 780                 


Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys 
785                 790                 795                 800 


Ser Arg Met Lys Arg Met Ala His Arg Leu Gly Glu Lys Met Leu Asn 
                805                 810                 815     


Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln 
            820                 825                 830         


Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser Asp 
        835                 840                 845             


Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser His 
    850                 855                 860                 


Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe His 
865                 870                 875                 880 


Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe 
                885                 890                 895     


Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro Ile 
            900                 905                 910         


Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile 
        915                 920                 925             


Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln 
    930                 935                 940                 


Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg Val 
945                 950                 955                 960 


Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu Lys 
                965                 970                 975     


Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met Ile 
            980                 985                 990         


His Tyr Gln Ala Val Val Val Leu  Glu Asn Leu Asn Phe  Gly Phe Lys 
        995                 1000                 1005             


Ser Lys  Arg Thr Gly Ile Ala  Glu Lys Ala Val Tyr  Gln Gln Phe 
    1010                 1015                 1020             


Glu Lys  Met Leu Ile Asp Lys  Leu Asn Cys Leu Val  Leu Lys Asp 
    1025                 1030                 1035             


Tyr Pro  Ala Glu Lys Val Gly  Gly Val Leu Asn Pro  Tyr Gln Leu 
    1040                 1045                 1050             


Thr Asp  Gln Phe Thr Ser Phe  Ala Lys Met Gly Thr  Gln Ser Gly 
    1055                 1060                 1065             


Phe Leu  Phe Tyr Val Pro Ala  Pro Tyr Thr Ser Lys  Ile Asp Pro 
    1070                 1075                 1080             


Leu Thr  Gly Phe Val Asp Pro  Phe Val Trp Lys Thr  Ile Lys Asn 
    1085                 1090                 1095             


His Glu  Ser Arg Lys His Phe  Leu Glu Gly Phe Asp  Phe Leu His 
    1100                 1105                 1110             


Tyr Asp  Val Lys Thr Gly Asp  Phe Ile Leu His Phe  Lys Met Asn 
    1115                 1120                 1125             


Arg Asn  Leu Ser Phe Gln Arg  Gly Leu Pro Gly Phe  Met Pro Ala 
    1130                 1135                 1140             


Trp Asp  Ile Val Phe Glu Lys  Asn Glu Thr Gln Phe  Asp Ala Lys 
    1145                 1150                 1155             


Gly Thr  Pro Phe Ile Ala Gly  Lys Arg Ile Val Pro  Val Ile Glu 
    1160                 1165                 1170             


Asn His  Arg Phe Thr Gly Arg  Tyr Arg Asp Leu Tyr  Pro Ala Asn 
    1175                 1180                 1185             


Glu Leu  Ile Ala Leu Leu Glu  Glu Lys Gly Ile Val  Phe Arg Asp 
    1190                 1195                 1200             


Gly Ser  Asn Ile Leu Pro Lys  Leu Leu Glu Asn Asp  Asp Ser His 
    1205                 1210                 1215             


Ala Ile  Asp Thr Met Val Ala  Leu Ile Arg Ser Val  Leu Gln Met 
    1220                 1225                 1230             


Arg Asn  Ser Asn Ala Ala Thr  Gly Glu Asp Tyr Ile  Asn Ser Pro 
    1235                 1240                 1245             


Val Arg  Asp Leu Asn Gly Val  Cys Phe Asp Ser Arg  Phe Gln Asn 
    1250                 1255                 1260             


Pro Glu  Trp Pro Met Asp Ala  Asp Ala Asn Gly Ala  Tyr His Ile 
    1265                 1270                 1275             


Ala Leu  Lys Gly Gln Leu Leu  Leu Asn His Leu Lys  Glu Ser Lys 
    1280                 1285                 1290             


Asp Leu  Lys Leu Gln Asn Gly  Ile Ser Asn Gln Asp  Trp Leu Ala 
    1295                 1300                 1305             


Tyr Ile  Gln Glu Leu Arg Asn  Lys Arg Pro Ala Ala  Thr Lys Lys 
    1310                 1315                 1320             


Ala Gly  Gln Ala Lys Lys Lys  Lys Gly 
    1325                 1330         


<210>  1001
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-sNLS-sNLS

<400>  1001

Met Lys His His His His His His Met Thr Gln Phe Glu Gly Phe Thr 
1               5                   10                  15      


Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln 
            20                  25                  30          


Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp 
        35                  40                  45              


Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg 
    50                  55                  60                  


Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu Asp 
65                  70                  75                  80  


Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr 
                85                  90                  95      


Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn 
            100                 105                 110         


Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala 
        115                 120                 125             


Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu 
    130                 135                 140                 


Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr Thr 
145                 150                 155                 160 


Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr 
                165                 170                 175     


Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu Asp 
            180                 185                 190         


Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro Lys 
        195                 200                 205             


Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val Pro 
    210                 215                 220                 


Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile Phe 
225                 230                 235                 240 


Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln 
                245                 250                 255     


Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly 
            260                 265                 270         


Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu Val 
        275                 280                 285             


Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile Ala 
    290                 295                 300                 


Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp 
305                 310                 315                 320 


Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu 
                325                 330                 335     


Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn 
            340                 345                 350         


Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp 
        355                 360                 365             


Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser Ser 
    370                 375                 380                 


Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg 
385                 390                 395                 400 


Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys 
                405                 410                 415     


Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile Ile 
            420                 425                 430         


Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser 
        435                 440                 445             


Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro Thr 
    450                 455                 460                 


Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp 
465                 470                 475                 480 


Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp Glu 
                485                 490                 495     


Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys 
            500                 505                 510         


Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr 
        515                 520                 525             


Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe Gln 
    530                 535                 540                 


Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu Lys Asn Asn 
545                 550                 555                 560 


Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met 
                565                 570                 575     


Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu 
            580                 585                 590         


Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp 
        595                 600                 605             


Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala Val Thr 
    610                 615                 620                 


Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe 
625                 630                 635                 640 


Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro 
                645                 650                 655     


Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly 
            660                 665                 670         


Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe Thr 
        675                 680                 685             


Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser 
    690                 695                 700                 


Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala 
705                 710                 715                 720 


Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala Glu 
                725                 730                 735     


Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln 
            740                 745                 750         


Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn Leu 
        755                 760                 765             


His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys 
    770                 775                 780                 


Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys 
785                 790                 795                 800 


Ser Arg Met Lys Arg Met Ala His Arg Leu Gly Glu Lys Met Leu Asn 
                805                 810                 815     


Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln 
            820                 825                 830         


Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser Asp 
        835                 840                 845             


Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser His 
    850                 855                 860                 


Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe His 
865                 870                 875                 880 


Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe 
                885                 890                 895     


Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro Ile 
            900                 905                 910         


Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile 
        915                 920                 925             


Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln 
    930                 935                 940                 


Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg Val 
945                 950                 955                 960 


Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu Lys 
                965                 970                 975     


Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met Ile 
            980                 985                 990         


His Tyr Gln Ala Val Val Val Leu  Glu Asn Leu Asn Phe  Gly Phe Lys 
        995                 1000                 1005             


Ser Lys  Arg Thr Gly Ile Ala  Glu Lys Ala Val Tyr  Gln Gln Phe 
    1010                 1015                 1020             


Glu Lys  Met Leu Ile Asp Lys  Leu Asn Cys Leu Val  Leu Lys Asp 
    1025                 1030                 1035             


Tyr Pro  Ala Glu Lys Val Gly  Gly Val Leu Asn Pro  Tyr Gln Leu 
    1040                 1045                 1050             


Thr Asp  Gln Phe Thr Ser Phe  Ala Lys Met Gly Thr  Gln Ser Gly 
    1055                 1060                 1065             


Phe Leu  Phe Tyr Val Pro Ala  Pro Tyr Thr Ser Lys  Ile Asp Pro 
    1070                 1075                 1080             


Leu Thr  Gly Phe Val Asp Pro  Phe Val Trp Lys Thr  Ile Lys Asn 
    1085                 1090                 1095             


His Glu  Ser Arg Lys His Phe  Leu Glu Gly Phe Asp  Phe Leu His 
    1100                 1105                 1110             


Tyr Asp  Val Lys Thr Gly Asp  Phe Ile Leu His Phe  Lys Met Asn 
    1115                 1120                 1125             


Arg Asn  Leu Ser Phe Gln Arg  Gly Leu Pro Gly Phe  Met Pro Ala 
    1130                 1135                 1140             


Trp Asp  Ile Val Phe Glu Lys  Asn Glu Thr Gln Phe  Asp Ala Lys 
    1145                 1150                 1155             


Gly Thr  Pro Phe Ile Ala Gly  Lys Arg Ile Val Pro  Val Ile Glu 
    1160                 1165                 1170             


Asn His  Arg Phe Thr Gly Arg  Tyr Arg Asp Leu Tyr  Pro Ala Asn 
    1175                 1180                 1185             


Glu Leu  Ile Ala Leu Leu Glu  Glu Lys Gly Ile Val  Phe Arg Asp 
    1190                 1195                 1200             


Gly Ser  Asn Ile Leu Pro Lys  Leu Leu Glu Asn Asp  Asp Ser His 
    1205                 1210                 1215             


Ala Ile  Asp Thr Met Val Ala  Leu Ile Arg Ser Val  Leu Gln Met 
    1220                 1225                 1230             


Arg Asn  Ser Asn Ala Ala Thr  Gly Glu Asp Tyr Ile  Asn Ser Pro 
    1235                 1240                 1245             


Val Arg  Asp Leu Asn Gly Val  Cys Phe Asp Ser Arg  Phe Gln Asn 
    1250                 1255                 1260             


Pro Glu  Trp Pro Met Asp Ala  Asp Ala Asn Gly Ala  Tyr His Ile 
    1265                 1270                 1275             


Ala Leu  Lys Gly Gln Leu Leu  Leu Asn His Leu Lys  Glu Ser Lys 
    1280                 1285                 1290             


Asp Leu  Lys Leu Gln Asn Gly  Ile Ser Asn Gln Asp  Trp Leu Ala 
    1295                 1300                 1305             


Tyr Ile  Gln Glu Leu Arg Asn  Gly Ser Pro Lys Lys  Lys Arg Lys 
    1310                 1315                 1320             


Val Gly  Ser Pro Lys Lys Lys  Arg Lys Val 
    1325                 1330             


<210>  1002
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 gRNA (OLI13620) targeting domain

<400>  1002
ccuugucaag gcuauugguc                                                   20


<210>  1003
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 gRNA (OLI13620) targeting domain

<400>  1003
ccttgtcaag gctattggtc                                                   20


<210>  1004
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 gRNA (OLI13620) targeting domain plus PAM

<400>  1004
uuugccuugu caaggcuauu gguc                                              24


<210>  1005
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 gRNA (OLI13620) targeting domain plus PAM

<400>  1005
tttgccttgt caaggctatt ggtc                                              24


<210>  1006
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nucleoplasmin NLS

<400>  1006

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  1007
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Simian Virus 40 NLS

<400>  1007

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  1008
<211>  1324
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-sNLS


<220>
<221>  MISC_FEATURE
<222>  (3)..(8)
<223>  His tag

<220>
<221>  MISC_FEATURE
<222>  (1318)..(1324)
<223>  SV40 NLS

<400>  1008

Met Lys His His His His His His Met Thr Gln Phe Glu Gly Phe Thr 
1               5                   10                  15      


Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln 
            20                  25                  30          


Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp 
        35                  40                  45              


Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg 
    50                  55                  60                  


Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu Asp 
65                  70                  75                  80  


Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr 
                85                  90                  95      


Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn 
            100                 105                 110         


Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala 
        115                 120                 125             


Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu 
    130                 135                 140                 


Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr Thr 
145                 150                 155                 160 


Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr 
                165                 170                 175     


Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu Asp 
            180                 185                 190         


Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro Lys 
        195                 200                 205             


Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val Pro 
    210                 215                 220                 


Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile Phe 
225                 230                 235                 240 


Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln 
                245                 250                 255     


Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly 
            260                 265                 270         


Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu Val 
        275                 280                 285             


Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile Ala 
    290                 295                 300                 


Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp 
305                 310                 315                 320 


Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu 
                325                 330                 335     


Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn 
            340                 345                 350         


Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp 
        355                 360                 365             


Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser Ser 
    370                 375                 380                 


Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg 
385                 390                 395                 400 


Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys 
                405                 410                 415     


Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile Ile 
            420                 425                 430         


Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser 
        435                 440                 445             


Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro Thr 
    450                 455                 460                 


Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp 
465                 470                 475                 480 


Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp Glu 
                485                 490                 495     


Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys 
            500                 505                 510         


Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr 
        515                 520                 525             


Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe Gln 
    530                 535                 540                 


Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu Lys Asn Asn 
545                 550                 555                 560 


Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met 
                565                 570                 575     


Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu 
            580                 585                 590         


Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp 
        595                 600                 605             


Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala Val Thr 
    610                 615                 620                 


Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe 
625                 630                 635                 640 


Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro 
                645                 650                 655     


Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly 
            660                 665                 670         


Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe Thr 
        675                 680                 685             


Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser 
    690                 695                 700                 


Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala 
705                 710                 715                 720 


Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala Glu 
                725                 730                 735     


Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln 
            740                 745                 750         


Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn Leu 
        755                 760                 765             


His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys 
    770                 775                 780                 


Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys 
785                 790                 795                 800 


Ser Arg Met Lys Arg Met Ala His Arg Leu Gly Glu Lys Met Leu Asn 
                805                 810                 815     


Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln 
            820                 825                 830         


Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser Asp 
        835                 840                 845             


Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser His 
    850                 855                 860                 


Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe His 
865                 870                 875                 880 


Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe 
                885                 890                 895     


Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro Ile 
            900                 905                 910         


Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile 
        915                 920                 925             


Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln 
    930                 935                 940                 


Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg Val 
945                 950                 955                 960 


Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu Lys 
                965                 970                 975     


Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met Ile 
            980                 985                 990         


His Tyr Gln Ala Val Val Val Leu  Glu Asn Leu Asn Phe  Gly Phe Lys 
        995                 1000                 1005             


Ser Lys  Arg Thr Gly Ile Ala  Glu Lys Ala Val Tyr  Gln Gln Phe 
    1010                 1015                 1020             


Glu Lys  Met Leu Ile Asp Lys  Leu Asn Cys Leu Val  Leu Lys Asp 
    1025                 1030                 1035             


Tyr Pro  Ala Glu Lys Val Gly  Gly Val Leu Asn Pro  Tyr Gln Leu 
    1040                 1045                 1050             


Thr Asp  Gln Phe Thr Ser Phe  Ala Lys Met Gly Thr  Gln Ser Gly 
    1055                 1060                 1065             


Phe Leu  Phe Tyr Val Pro Ala  Pro Tyr Thr Ser Lys  Ile Asp Pro 
    1070                 1075                 1080             


Leu Thr  Gly Phe Val Asp Pro  Phe Val Trp Lys Thr  Ile Lys Asn 
    1085                 1090                 1095             


His Glu  Ser Arg Lys His Phe  Leu Glu Gly Phe Asp  Phe Leu His 
    1100                 1105                 1110             


Tyr Asp  Val Lys Thr Gly Asp  Phe Ile Leu His Phe  Lys Met Asn 
    1115                 1120                 1125             


Arg Asn  Leu Ser Phe Gln Arg  Gly Leu Pro Gly Phe  Met Pro Ala 
    1130                 1135                 1140             


Trp Asp  Ile Val Phe Glu Lys  Asn Glu Thr Gln Phe  Asp Ala Lys 
    1145                 1150                 1155             


Gly Thr  Pro Phe Ile Ala Gly  Lys Arg Ile Val Pro  Val Ile Glu 
    1160                 1165                 1170             


Asn His  Arg Phe Thr Gly Arg  Tyr Arg Asp Leu Tyr  Pro Ala Asn 
    1175                 1180                 1185             


Glu Leu  Ile Ala Leu Leu Glu  Glu Lys Gly Ile Val  Phe Arg Asp 
    1190                 1195                 1200             


Gly Ser  Asn Ile Leu Pro Lys  Leu Leu Glu Asn Asp  Asp Ser His 
    1205                 1210                 1215             


Ala Ile  Asp Thr Met Val Ala  Leu Ile Arg Ser Val  Leu Gln Met 
    1220                 1225                 1230             


Arg Asn  Ser Asn Ala Ala Thr  Gly Glu Asp Tyr Ile  Asn Ser Pro 
    1235                 1240                 1245             


Val Arg  Asp Leu Asn Gly Val  Cys Phe Asp Ser Arg  Phe Gln Asn 
    1250                 1255                 1260             


Pro Glu  Trp Pro Met Asp Ala  Asp Ala Asn Gly Ala  Tyr His Ile 
    1265                 1270                 1275             


Ala Leu  Lys Gly Gln Leu Leu  Leu Asn His Leu Lys  Glu Ser Lys 
    1280                 1285                 1290             


Asp Leu  Lys Leu Gln Asn Gly  Ile Ser Asn Gln Asp  Trp Leu Ala 
    1295                 1300                 1305             


Tyr Ile  Gln Glu Leu Arg Asn  Gly Ser Pro Lys Lys  Lys Arg Lys 
    1310                 1315                 1320             


Val 
    


<210>  1009
<211>  1326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-sNLS-AsCpf1


<220>
<221>  MISC_FEATURE
<222>  (3)..(8)
<223>  His tag

<220>
<221>  MISC_FEATURE
<222>  (11)..(17)
<223>  SV40 NLS

<400>  1009

Met Lys His His His His His His Gly Ser Pro Lys Lys Lys Arg Lys 
1               5                   10                  15      


Val Gly Ser Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val 
            20                  25                  30          


Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys 
        35                  40                  45              


His Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp 
    50                  55                  60                  


His Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr 
65                  70                  75                  80  


Ala Asp Gln Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser 
                85                  90                  95      


Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn 
            100                 105                 110         


Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr 
        115                 120                 125             


Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His 
    130                 135                 140                 


Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys 
145                 150                 155                 160 


Val Leu Lys Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala 
                165                 170                 175     


Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr 
            180                 185                 190         


Glu Asn Arg Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile 
        195                 200                 205             


Pro His Arg Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys 
    210                 215                 220                 


His Ile Phe Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His 
225                 230                 235                 240 


Phe Glu Asn Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile 
                245                 250                 255     


Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr 
            260                 265                 270         


Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala 
        275                 280                 285             


Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile 
    290                 295                 300                 


Gln Lys Asn Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg 
305                 310                 315                 320 


Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser 
                325                 330                 335     


Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe 
            340                 345                 350         


Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala 
        355                 360                 365             


Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe 
    370                 375                 380                 


Ile Ser His Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His 
385                 390                 395                 400 


Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu 
                405                 410                 415     


Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu 
            420                 425                 430         


Lys His Glu Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys 
        435                 440                 445             


Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His 
    450                 455                 460                 


Ala His Ala Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln 
465                 470                 475                 480 


Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu 
                485                 490                 495     


Tyr His Leu Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp 
            500                 505                 510         


Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro 
        515                 520                 525             


Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro 
    530                 535                 540                 


Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala 
545                 550                 555                 560 


Ser Gly Trp Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe 
                565                 570                 575     


Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly 
            580                 585                 590         


Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly 
        595                 600                 605             


Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile 
    610                 615                 620                 


Pro Lys Cys Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr 
625                 630                 635                 640 


His Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu 
                645                 650                 655     


Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys 
            660                 665                 670         


Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr 
        675                 680                 685             


Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser 
    690                 695                 700                 


Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser 
705                 710                 715                 720 


Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu 
                725                 730                 735     


Leu Tyr His Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp 
            740                 745                 750         


Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp 
        755                 760                 765             


Phe Ala Lys Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp 
    770                 775                 780                 


Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu 
785                 790                 795                 800 


Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg 
                805                 810                 815     


Met Ala His Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp 
            820                 825                 830         


Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr 
        835                 840                 845             


Val Asn His Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu 
    850                 855                 860                 


Leu Pro Asn Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp 
865                 870                 875                 880 


Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu 
                885                 890                 895     


Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn 
            900                 905                 910         


Ala Tyr Leu Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg 
        915                 920                 925             


Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys 
    930                 935                 940                 


Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln 
945                 950                 955                 960 


Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala 
                965                 970                 975     


Trp Ser Val Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser 
            980                 985                 990         


Gln Val Ile His Glu Ile Val Asp  Leu Met Ile His Tyr  Gln Ala Val 
        995                 1000                 1005             


Val Val  Leu Glu Asn Leu Asn  Phe Gly Phe Lys Ser  Lys Arg Thr 
    1010                 1015                 1020             


Gly Ile  Ala Glu Lys Ala Val  Tyr Gln Gln Phe Glu  Lys Met Leu 
    1025                 1030                 1035             


Ile Asp  Lys Leu Asn Cys Leu  Val Leu Lys Asp Tyr  Pro Ala Glu 
    1040                 1045                 1050             


Lys Val  Gly Gly Val Leu Asn  Pro Tyr Gln Leu Thr  Asp Gln Phe 
    1055                 1060                 1065             


Thr Ser  Phe Ala Lys Met Gly  Thr Gln Ser Gly Phe  Leu Phe Tyr 
    1070                 1075                 1080             


Val Pro  Ala Pro Tyr Thr Ser  Lys Ile Asp Pro Leu  Thr Gly Phe 
    1085                 1090                 1095             


Val Asp  Pro Phe Val Trp Lys  Thr Ile Lys Asn His  Glu Ser Arg 
    1100                 1105                 1110             


Lys His  Phe Leu Glu Gly Phe  Asp Phe Leu His Tyr  Asp Val Lys 
    1115                 1120                 1125             


Thr Gly  Asp Phe Ile Leu His  Phe Lys Met Asn Arg  Asn Leu Ser 
    1130                 1135                 1140             


Phe Gln  Arg Gly Leu Pro Gly  Phe Met Pro Ala Trp  Asp Ile Val 
    1145                 1150                 1155             


Phe Glu  Lys Asn Glu Thr Gln  Phe Asp Ala Lys Gly  Thr Pro Phe 
    1160                 1165                 1170             


Ile Ala  Gly Lys Arg Ile Val  Pro Val Ile Glu Asn  His Arg Phe 
    1175                 1180                 1185             


Thr Gly  Arg Tyr Arg Asp Leu  Tyr Pro Ala Asn Glu  Leu Ile Ala 
    1190                 1195                 1200             


Leu Leu  Glu Glu Lys Gly Ile  Val Phe Arg Asp Gly  Ser Asn Ile 
    1205                 1210                 1215             


Leu Pro  Lys Leu Leu Glu Asn  Asp Asp Ser His Ala  Ile Asp Thr 
    1220                 1225                 1230             


Met Val  Ala Leu Ile Arg Ser  Val Leu Gln Met Arg  Asn Ser Asn 
    1235                 1240                 1245             


Ala Ala  Thr Gly Glu Asp Tyr  Ile Asn Ser Pro Val  Arg Asp Leu 
    1250                 1255                 1260             


Asn Gly  Val Cys Phe Asp Ser  Arg Phe Gln Asn Pro  Glu Trp Pro 
    1265                 1270                 1275             


Met Asp  Ala Asp Ala Asn Gly  Ala Tyr His Ile Ala  Leu Lys Gly 
    1280                 1285                 1290             


Gln Leu  Leu Leu Asn His Leu  Lys Glu Ser Lys Asp  Leu Lys Leu 
    1295                 1300                 1305             


Gln Asn  Gly Ile Ser Asn Gln  Asp Trp Leu Ala Tyr  Ile Gln Glu 
    1310                 1315                 1320             


Leu Arg  Asn 
    1325     


<210>  1010
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-sNLS-sNLS-AsCpf1


<220>
<221>  MISC_FEATURE
<222>  (3)..(8)
<223>  His tag

<220>
<221>  MISC_FEATURE
<222>  (11)..(17)
<223>  SV40 NLS

<220>
<221>  MISC_FEATURE
<222>  (20)..(26)
<223>  SV40 NLS

<400>  1010

Met Lys His His His His His His Gly Ser Pro Lys Lys Lys Arg Lys 
1               5                   10                  15      


Val Gly Ser Pro Lys Lys Lys Arg Lys Val Met Thr Gln Phe Glu Gly 
            20                  25                  30          


Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile 
        35                  40                  45              


Pro Gln Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu 
    50                  55                  60                  


Glu Asp Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile 
65                  70                  75                  80  


Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln 
                85                  90                  95      


Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu 
            100                 105                 110         


Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr 
        115                 120                 125             


Arg Asn Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr 
    130                 135                 140                 


Asp Ala Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys 
145                 150                 155                 160 


Ala Glu Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr 
                165                 170                 175     


Thr Thr Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr 
            180                 185                 190         


Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala 
        195                 200                 205             


Glu Asp Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe 
    210                 215                 220                 


Pro Lys Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala 
225                 230                 235                 240 


Val Pro Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly 
                245                 250                 255     


Ile Phe Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr 
            260                 265                 270         


Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu 
        275                 280                 285             


Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn 
    290                 295                 300                 


Glu Val Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile 
305                 310                 315                 320 


Ile Ala Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu 
                325                 330                 335     


Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp 
            340                 345                 350         


Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn 
        355                 360                 365             


Glu Asn Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser 
    370                 375                 380                 


Ile Asp Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile 
385                 390                 395                 400 


Ser Ser Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr 
                405                 410                 415     


Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys 
            420                 425                 430         


Glu Lys Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu 
        435                 440                 445             


Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys 
    450                 455                 460                 


Thr Ser Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu 
465                 470                 475                 480 


Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln 
                485                 490                 495     


Leu Asp Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val 
            500                 505                 510         


Asp Glu Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly 
        515                 520                 525             


Ile Lys Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg 
    530                 535                 540                 


Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn 
545                 550                 555                 560 


Phe Gln Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu Lys 
                565                 570                 575     


Asn Asn Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly 
            580                 585                 590         


Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro 
        595                 600                 605             


Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe 
    610                 615                 620                 


Pro Asp Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala 
625                 630                 635                 640 


Val Thr Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn 
                645                 650                 655     


Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn 
            660                 665                 670         


Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys 
        675                 680                 685             


Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp 
    690                 695                 700                 


Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp 
705                 710                 715                 720 


Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr 
                725                 730                 735     


Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile 
            740                 745                 750         


Ala Glu Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu 
        755                 760                 765             


Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro 
    770                 775                 780                 


Asn Leu His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu 
785                 790                 795                 800 


Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg 
                805                 810                 815     


Pro Lys Ser Arg Met Lys Arg Met Ala His Arg Leu Gly Glu Lys Met 
            820                 825                 830         


Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu 
        835                 840                 845             


Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu 
    850                 855                 860                 


Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val 
865                 870                 875                 880 


Ser His Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe 
                885                 890                 895     


Phe His Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser 
            900                 905                 910         


Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr 
        915                 920                 925             


Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr 
    930                 935                 940                 


Val Ile Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr 
945                 950                 955                 960 


Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu 
                965                 970                 975     


Arg Val Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp 
            980                 985                 990         


Leu Lys Gln Gly Tyr Leu Ser Gln  Val Ile His Glu Ile  Val Asp Leu 
        995                 1000                 1005             


Met Ile  His Tyr Gln Ala Val  Val Val Leu Glu Asn  Leu Asn Phe 
    1010                 1015                 1020             


Gly Phe  Lys Ser Lys Arg Thr  Gly Ile Ala Glu Lys  Ala Val Tyr 
    1025                 1030                 1035             


Gln Gln  Phe Glu Lys Met Leu  Ile Asp Lys Leu Asn  Cys Leu Val 
    1040                 1045                 1050             


Leu Lys  Asp Tyr Pro Ala Glu  Lys Val Gly Gly Val  Leu Asn Pro 
    1055                 1060                 1065             


Tyr Gln  Leu Thr Asp Gln Phe  Thr Ser Phe Ala Lys  Met Gly Thr 
    1070                 1075                 1080             


Gln Ser  Gly Phe Leu Phe Tyr  Val Pro Ala Pro Tyr  Thr Ser Lys 
    1085                 1090                 1095             


Ile Asp  Pro Leu Thr Gly Phe  Val Asp Pro Phe Val  Trp Lys Thr 
    1100                 1105                 1110             


Ile Lys  Asn His Glu Ser Arg  Lys His Phe Leu Glu  Gly Phe Asp 
    1115                 1120                 1125             


Phe Leu  His Tyr Asp Val Lys  Thr Gly Asp Phe Ile  Leu His Phe 
    1130                 1135                 1140             


Lys Met  Asn Arg Asn Leu Ser  Phe Gln Arg Gly Leu  Pro Gly Phe 
    1145                 1150                 1155             


Met Pro  Ala Trp Asp Ile Val  Phe Glu Lys Asn Glu  Thr Gln Phe 
    1160                 1165                 1170             


Asp Ala  Lys Gly Thr Pro Phe  Ile Ala Gly Lys Arg  Ile Val Pro 
    1175                 1180                 1185             


Val Ile  Glu Asn His Arg Phe  Thr Gly Arg Tyr Arg  Asp Leu Tyr 
    1190                 1195                 1200             


Pro Ala  Asn Glu Leu Ile Ala  Leu Leu Glu Glu Lys  Gly Ile Val 
    1205                 1210                 1215             


Phe Arg  Asp Gly Ser Asn Ile  Leu Pro Lys Leu Leu  Glu Asn Asp 
    1220                 1225                 1230             


Asp Ser  His Ala Ile Asp Thr  Met Val Ala Leu Ile  Arg Ser Val 
    1235                 1240                 1245             


Leu Gln  Met Arg Asn Ser Asn  Ala Ala Thr Gly Glu  Asp Tyr Ile 
    1250                 1255                 1260             


Asn Ser  Pro Val Arg Asp Leu  Asn Gly Val Cys Phe  Asp Ser Arg 
    1265                 1270                 1275             


Phe Gln  Asn Pro Glu Trp Pro  Met Asp Ala Asp Ala  Asn Gly Ala 
    1280                 1285                 1290             


Tyr His  Ile Ala Leu Lys Gly  Gln Leu Leu Leu Asn  His Leu Lys 
    1295                 1300                 1305             


Glu Ser  Lys Asp Leu Lys Leu  Gln Asn Gly Ile Ser  Asn Gln Asp 
    1310                 1315                 1320             


Trp Leu  Ala Tyr Ile Gln Glu  Leu Arg Asn 
    1325                 1330             


<210>  1011
<211>  1327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sNLS-sNLS-AsCpf1


<220>
<221>  MISC_FEATURE
<222>  (5)..(11)
<223>  SV40 NLS

<220>
<221>  MISC_FEATURE
<222>  (14)..(20)
<223>  SV40 NLS

<400>  1011

Met Lys Gly Ser Pro Lys Lys Lys Arg Lys Val Gly Ser Pro Lys Lys 
1               5                   10                  15      


Lys Arg Lys Val Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln 
            20                  25                  30          


Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu 
        35                  40                  45              


Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn 
    50                  55                  60                  


Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr 
65                  70                  75                  80  


Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu 
                85                  90                  95      


Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg 
            100                 105                 110         


Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp 
        115                 120                 125             


Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg 
    130                 135                 140                 


His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly 
145                 150                 155                 160 


Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn 
                165                 170                 175     


Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe 
            180                 185                 190         


Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala 
        195                 200                 205             


Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn 
    210                 215                 220                 


Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu 
225                 230                 235                 240 


His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser 
                245                 250                 255     


Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln 
            260                 265                 270         


Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu 
        275                 280                 285             


Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala 
    290                 295                 300                 


Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His 
305                 310                 315                 320 


Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu 
                325                 330                 335     


Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser 
            340                 345                 350         


Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr 
        355                 360                 365             


Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile 
    370                 375                 380                 


Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp 
385                 390                 395                 400 


His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu 
                405                 410                 415     


Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser 
            420                 425                 430         


Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly 
        435                 440                 445             


Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser 
    450                 455                 460                 


His Ala His Ala Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys 
465                 470                 475                 480 


Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly 
                485                 490                 495     


Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val 
            500                 505                 510         


Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu 
        515                 520                 525             


Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys 
    530                 535                 540                 


Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu 
545                 550                 555                 560 


Ala Ser Gly Trp Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu 
                565                 570                 575     


Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys 
            580                 585                 590         


Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu 
        595                 600                 605             


Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met 
    610                 615                 620                 


Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln 
625                 630                 635                 640 


Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu 
                645                 650                 655     


Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro 
            660                 665                 670         


Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly 
        675                 680                 685             


Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu 
    690                 695                 700                 


Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro 
705                 710                 715                 720 


Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro 
                725                 730                 735     


Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met 
            740                 745                 750         


Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys 
        755                 760                 765             


Asp Phe Ala Lys Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr 
    770                 775                 780                 


Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys 
785                 790                 795                 800 


Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys 
                805                 810                 815     


Arg Met Ala His Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys 
            820                 825                 830         


Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp 
        835                 840                 845             


Tyr Val Asn His Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala 
    850                 855                 860                 


Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys 
865                 870                 875                 880 


Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr 
                885                 890                 895     


Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val 
            900                 905                 910         


Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp 
        915                 920                 925             


Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly 
    930                 935                 940                 


Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr 
945                 950                 955                 960 


Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln 
                965                 970                 975     


Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu 
            980                 985                 990         


Ser Gln Val Ile His Glu Ile Val  Asp Leu Met Ile His  Tyr Gln Ala 
        995                 1000                 1005             


Val Val  Val Leu Glu Asn Leu  Asn Phe Gly Phe Lys  Ser Lys Arg 
    1010                 1015                 1020             


Thr Gly  Ile Ala Glu Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met 
    1025                 1030                 1035             


Leu Ile  Asp Lys Leu Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala 
    1040                 1045                 1050             


Glu Lys  Val Gly Gly Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln 
    1055                 1060                 1065             


Phe Thr  Ser Phe Ala Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe 
    1070                 1075                 1080             


Tyr Val  Pro Ala Pro Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly 
    1085                 1090                 1095             


Phe Val  Asp Pro Phe Val Trp  Lys Thr Ile Lys Asn  His Glu Ser 
    1100                 1105                 1110             


Arg Lys  His Phe Leu Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val 
    1115                 1120                 1125             


Lys Thr  Gly Asp Phe Ile Leu  His Phe Lys Met Asn  Arg Asn Leu 
    1130                 1135                 1140             


Ser Phe  Gln Arg Gly Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile 
    1145                 1150                 1155             


Val Phe  Glu Lys Asn Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro 
    1160                 1165                 1170             


Phe Ile  Ala Gly Lys Arg Ile  Val Pro Val Ile Glu  Asn His Arg 
    1175                 1180                 1185             


Phe Thr  Gly Arg Tyr Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile 
    1190                 1195                 1200             


Ala Leu  Leu Glu Glu Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn 
    1205                 1210                 1215             


Ile Leu  Pro Lys Leu Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp 
    1220                 1225                 1230             


Thr Met  Val Ala Leu Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser 
    1235                 1240                 1245             


Asn Ala  Ala Thr Gly Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp 
    1250                 1255                 1260             


Leu Asn  Gly Val Cys Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp 
    1265                 1270                 1275             


Pro Met  Asp Ala Asp Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys 
    1280                 1285                 1290             


Gly Gln  Leu Leu Leu Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys 
    1295                 1300                 1305             


Leu Gln  Asn Gly Ile Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln 
    1310                 1315                 1320             


Glu Leu  Arg Asn 
    1325         


<210>  1012
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-sNLS-AsCpf1-sNLS


<220>
<221>  MISC_FEATURE
<222>  (3)..(8)
<223>  His tag

<220>
<221>  MISC_FEATURE
<222>  (10)..(16)
<223>  SV40 NLS

<220>
<221>  MISC_FEATURE
<222>  (1325)..(1331)
<223>  SV40 NLS

<400>  1012

Met Lys His His His His His His Met Pro Lys Lys Lys Arg Lys Val 
1               5                   10                  15      


Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
            20                  25                  30          


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
        35                  40                  45              


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
    50                  55                  60                  


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
65                  70                  75                  80  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
                85                  90                  95      


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
            100                 105                 110         


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
        115                 120                 125             


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
    130                 135                 140                 


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
145                 150                 155                 160 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
                165                 170                 175     


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
            180                 185                 190         


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
        195                 200                 205             


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
    210                 215                 220                 


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
225                 230                 235                 240 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
                245                 250                 255     


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
            260                 265                 270         


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
        275                 280                 285             


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
    290                 295                 300                 


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
305                 310                 315                 320 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
                325                 330                 335     


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
            340                 345                 350         


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
        355                 360                 365             


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
    370                 375                 380                 


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
385                 390                 395                 400 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
                405                 410                 415     


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
            420                 425                 430         


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
        435                 440                 445             


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
    450                 455                 460                 


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
465                 470                 475                 480 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
                485                 490                 495     


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
            500                 505                 510         


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
        515                 520                 525             


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
    530                 535                 540                 


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
545                 550                 555                 560 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
                565                 570                 575     


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
            580                 585                 590         


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
        595                 600                 605             


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
    610                 615                 620                 


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
625                 630                 635                 640 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
                645                 650                 655     


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
            660                 665                 670         


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
        675                 680                 685             


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
    690                 695                 700                 


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
705                 710                 715                 720 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
                725                 730                 735     


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
            740                 745                 750         


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
        755                 760                 765             


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
    770                 775                 780                 


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
785                 790                 795                 800 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
                805                 810                 815     


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
            820                 825                 830         


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
        835                 840                 845             


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
    850                 855                 860                 


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
865                 870                 875                 880 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
                885                 890                 895     


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
            900                 905                 910         


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
        915                 920                 925             


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
    930                 935                 940                 


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
945                 950                 955                 960 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
                965                 970                 975     


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
            980                 985                 990         


His Glu Ile Val Asp Leu Met Ile  His Tyr Gln Ala Val  Val Val Leu 
        995                 1000                 1005             


Glu Asn  Leu Asn Phe Gly Phe  Lys Ser Lys Arg Thr  Gly Ile Ala 
    1010                 1015                 1020             


Glu Lys  Ala Val Tyr Gln Gln  Phe Glu Lys Met Leu  Ile Asp Lys 
    1025                 1030                 1035             


Leu Asn  Cys Leu Val Leu Lys  Asp Tyr Pro Ala Glu  Lys Val Gly 
    1040                 1045                 1050             


Gly Val  Leu Asn Pro Tyr Gln  Leu Thr Asp Gln Phe  Thr Ser Phe 
    1055                 1060                 1065             


Ala Lys  Met Gly Thr Gln Ser  Gly Phe Leu Phe Tyr  Val Pro Ala 
    1070                 1075                 1080             


Pro Tyr  Thr Ser Lys Ile Asp  Pro Leu Thr Gly Phe  Val Asp Pro 
    1085                 1090                 1095             


Phe Val  Trp Lys Thr Ile Lys  Asn His Glu Ser Arg  Lys His Phe 
    1100                 1105                 1110             


Leu Glu  Gly Phe Asp Phe Leu  His Tyr Asp Val Lys  Thr Gly Asp 
    1115                 1120                 1125             


Phe Ile  Leu His Phe Lys Met  Asn Arg Asn Leu Ser  Phe Gln Arg 
    1130                 1135                 1140             


Gly Leu  Pro Gly Phe Met Pro  Ala Trp Asp Ile Val  Phe Glu Lys 
    1145                 1150                 1155             


Asn Glu  Thr Gln Phe Asp Ala  Lys Gly Thr Pro Phe  Ile Ala Gly 
    1160                 1165                 1170             


Lys Arg  Ile Val Pro Val Ile  Glu Asn His Arg Phe  Thr Gly Arg 
    1175                 1180                 1185             


Tyr Arg  Asp Leu Tyr Pro Ala  Asn Glu Leu Ile Ala  Leu Leu Glu 
    1190                 1195                 1200             


Glu Lys  Gly Ile Val Phe Arg  Asp Gly Ser Asn Ile  Leu Pro Lys 
    1205                 1210                 1215             


Leu Leu  Glu Asn Asp Asp Ser  His Ala Ile Asp Thr  Met Val Ala 
    1220                 1225                 1230             


Leu Ile  Arg Ser Val Leu Gln  Met Arg Asn Ser Asn  Ala Ala Thr 
    1235                 1240                 1245             


Gly Glu  Asp Tyr Ile Asn Ser  Pro Val Arg Asp Leu  Asn Gly Val 
    1250                 1255                 1260             


Cys Phe  Asp Ser Arg Phe Gln  Asn Pro Glu Trp Pro  Met Asp Ala 
    1265                 1270                 1275             


Asp Ala  Asn Gly Ala Tyr His  Ile Ala Leu Lys Gly  Gln Leu Leu 
    1280                 1285                 1290             


Leu Asn  His Leu Lys Glu Ser  Lys Asp Leu Lys Leu  Gln Asn Gly 
    1295                 1300                 1305             


Ile Ser  Asn Gln Asp Trp Leu  Ala Tyr Ile Gln Glu  Leu Arg Asn 
    1310                 1315                 1320             


Gly Pro  Lys Lys Lys Arg Lys  Val Gly 
    1325                 1330         


<210>  1013
<211>  1352
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-sNLS-sNLS-AsCpf1-sNLS-sNLS


<220>
<221>  MISC_FEATURE
<222>  (3)..(8)
<223>  His tag

<220>
<221>  MISC_FEATURE
<222>  (11)..(17)
<223>  SV40 NLS

<220>
<221>  MISC_FEATURE
<222>  (20)..(26)
<223>  SV40 NLS

<220>
<221>  MISC_FEATURE
<222>  (1337)..(1343)
<223>  SV40 NLS

<220>
<221>  MISC_FEATURE
<222>  (1346)..(1352)
<223>  SV40 NLS

<400>  1013

Met Gly His His His His His His Gly Ser Pro Lys Lys Lys Arg Lys 
1               5                   10                  15      


Val Gly Ser Pro Lys Lys Lys Arg Lys Val Gly Ser Thr Gln Phe Glu 
            20                  25                  30          


Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu 
        35                  40                  45              


Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile 
    50                  55                  60                  


Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile 
65                  70                  75                  80  


Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val 
                85                  90                  95      


Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys 
            100                 105                 110         


Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr 
        115                 120                 125             


Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu 
    130                 135                 140                 


Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe 
145                 150                 155                 160 


Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val 
                165                 170                 175     


Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe 
            180                 185                 190         


Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser 
        195                 200                 205             


Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn 
    210                 215                 220                 


Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr 
225                 230                 235                 240 


Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile 
                245                 250                 255     


Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe 
            260                 265                 270         


Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu 
        275                 280                 285             


Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu 
    290                 295                 300                 


Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His 
305                 310                 315                 320 


Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile 
                325                 330                 335     


Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser 
            340                 345                 350         


Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg 
        355                 360                 365             


Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn 
    370                 375                 380                 


Ser Ile Asp Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr 
385                 390                 395                 400 


Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu 
                405                 410                 415     


Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala 
            420                 425                 430         


Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln 
        435                 440                 445             


Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln 
    450                 455                 460                 


Lys Thr Ser Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro 
465                 470                 475                 480 


Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser 
                485                 490                 495     


Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala 
            500                 505                 510         


Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr 
        515                 520                 525             


Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala 
    530                 535                 540                 


Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu 
545                 550                 555                 560 


Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu 
                565                 570                 575     


Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu 
            580                 585                 590         


Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu 
        595                 600                 605             


Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr 
    610                 615                 620                 


Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys 
625                 630                 635                 640 


Ala Val Thr Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser 
                645                 650                 655     


Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu 
            660                 665                 670         


Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys 
        675                 680                 685             


Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile 
    690                 695                 700                 


Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile 
705                 710                 715                 720 


Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu 
                725                 730                 735     


Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg 
            740                 745                 750         


Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr 
        755                 760                 765             


Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys 
    770                 775                 780                 


Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn 
785                 790                 795                 800 


Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr 
                805                 810                 815     


Arg Pro Lys Ser Arg Met Lys Arg Met Ala Ala Arg Leu Gly Glu Lys 
            820                 825                 830         


Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr 
        835                 840                 845             


Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp 
    850                 855                 860                 


Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu 
865                 870                 875                 880 


Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe 
                885                 890                 895     


Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro 
            900                 905                 910         


Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu 
        915                 920                 925             


Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile 
    930                 935                 940                 


Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn 
945                 950                 955                 960 


Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys 
                965                 970                 975     


Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys 
            980                 985                 990         


Asp Leu Lys Gln Gly Tyr Leu Ser  Gln Val Ile His Glu  Ile Val Asp 
        995                 1000                 1005             


Leu Met  Ile His Tyr Gln Ala  Val Val Val Leu Glu  Asn Leu Asn 
    1010                 1015                 1020             


Phe Gly  Phe Lys Ser Lys Arg  Thr Gly Ile Ala Glu  Lys Ala Val 
    1025                 1030                 1035             


Tyr Gln  Gln Phe Glu Lys Met  Leu Ile Asp Lys Leu  Asn Cys Leu 
    1040                 1045                 1050             


Val Leu  Lys Asp Tyr Pro Ala  Glu Lys Val Gly Gly  Val Leu Asn 
    1055                 1060                 1065             


Pro Tyr  Gln Leu Thr Asp Gln  Phe Thr Ser Phe Ala  Lys Met Gly 
    1070                 1075                 1080             


Thr Gln  Ser Gly Phe Leu Phe  Tyr Val Pro Ala Pro  Tyr Thr Ser 
    1085                 1090                 1095             


Lys Ile  Asp Pro Leu Thr Gly  Phe Val Asp Pro Phe  Val Trp Lys 
    1100                 1105                 1110             


Thr Ile  Lys Asn His Glu Ser  Arg Lys His Phe Leu  Glu Gly Phe 
    1115                 1120                 1125             


Asp Phe  Leu His Tyr Asp Val  Lys Thr Gly Asp Phe  Ile Leu His 
    1130                 1135                 1140             


Phe Lys  Met Asn Arg Asn Leu  Ser Phe Gln Arg Gly  Leu Pro Gly 
    1145                 1150                 1155             


Phe Met  Pro Ala Trp Asp Ile  Val Phe Glu Lys Asn  Glu Thr Gln 
    1160                 1165                 1170             


Phe Asp  Ala Lys Gly Thr Pro  Phe Ile Ala Gly Lys  Arg Ile Val 
    1175                 1180                 1185             


Pro Val  Ile Glu Asn His Arg  Phe Thr Gly Arg Tyr  Arg Asp Leu 
    1190                 1195                 1200             


Tyr Pro  Ala Asn Glu Leu Ile  Ala Leu Leu Glu Glu  Lys Gly Ile 
    1205                 1210                 1215             


Val Phe  Arg Asp Gly Ser Asn  Ile Leu Pro Lys Leu  Leu Glu Asn 
    1220                 1225                 1230             


Asp Asp  Ser His Ala Ile Asp  Thr Met Val Ala Leu  Ile Arg Ser 
    1235                 1240                 1245             


Val Leu  Gln Met Arg Asn Ser  Asn Ala Ala Thr Gly  Glu Asp Tyr 
    1250                 1255                 1260             


Ile Asn  Ser Pro Val Arg Asp  Leu Asn Gly Val Cys  Phe Asp Ser 
    1265                 1270                 1275             


Arg Phe  Gln Asn Pro Glu Trp  Pro Met Asp Ala Asp  Ala Asn Gly 
    1280                 1285                 1290             


Ala Tyr  His Ile Ala Leu Lys  Gly Gln Leu Leu Leu  Asn His Leu 
    1295                 1300                 1305             


Lys Glu  Ser Lys Asp Leu Lys  Leu Gln Asn Gly Ile  Ser Asn Gln 
    1310                 1315                 1320             


Asp Trp  Leu Ala Tyr Ile Gln  Glu Leu Arg Asn Gly  Ser Pro Lys 
    1325                 1330                 1335             


Lys Lys  Arg Lys Val Gly Ser  Pro Lys Lys Lys Arg  Lys Val 
    1340                 1345                 1350         


<210>  1014
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-nNLS Cys-less


<220>
<221>  MISC_FEATURE
<222>  (3)..(8)
<223>  His tag

<220>
<221>  MISC_FEATURE
<222>  (1316)..(1331)
<223>  Nucleoplasmin NLS

<400>  1014

Met Lys His His His His His His Met Thr Gln Phe Glu Gly Phe Thr 
1               5                   10                  15      


Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln 
            20                  25                  30          


Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp 
        35                  40                  45              


Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg 
    50                  55                  60                  


Ile Tyr Lys Thr Tyr Ala Asp Gln Ser Leu Gln Leu Val Gln Leu Asp 
65                  70                  75                  80  


Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr 
                85                  90                  95      


Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn 
            100                 105                 110         


Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala 
        115                 120                 125             


Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu 
    130                 135                 140                 


Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr Thr 
145                 150                 155                 160 


Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr 
                165                 170                 175     


Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu Asp 
            180                 185                 190         


Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro Lys 
        195                 200                 205             


Phe Lys Glu Asn Ser His Ile Phe Thr Arg Leu Ile Thr Ala Val Pro 
    210                 215                 220                 


Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile Phe 
225                 230                 235                 240 


Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln 
                245                 250                 255     


Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly 
            260                 265                 270         


Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu Val 
        275                 280                 285             


Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile Ala 
    290                 295                 300                 


Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp 
305                 310                 315                 320 


Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu 
                325                 330                 335     


Val Ile Gln Ser Phe Ser Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn 
            340                 345                 350         


Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp 
        355                 360                 365             


Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser Ser 
    370                 375                 380                 


Ala Leu Ser Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg 
385                 390                 395                 400 


Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys 
                405                 410                 415     


Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile Ile 
            420                 425                 430         


Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser 
        435                 440                 445             


Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro Thr 
    450                 455                 460                 


Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp 
465                 470                 475                 480 


Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp Glu 
                485                 490                 495     


Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys 
            500                 505                 510         


Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr 
        515                 520                 525             


Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe Gln 
    530                 535                 540                 


Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu Lys Asn Asn 
545                 550                 555                 560 


Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met 
                565                 570                 575     


Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu 
            580                 585                 590         


Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp 
        595                 600                 605             


Ala Ala Lys Met Ile Pro Lys Ser Ser Thr Gln Leu Lys Ala Val Thr 
    610                 615                 620                 


Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe 
625                 630                 635                 640 


Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro 
                645                 650                 655     


Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly 
            660                 665                 670         


Asp Gln Lys Gly Tyr Arg Glu Ala Leu Ser Lys Trp Ile Asp Phe Thr 
        675                 680                 685             


Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser 
    690                 695                 700                 


Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala 
705                 710                 715                 720 


Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala Glu 
                725                 730                 735     


Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln 
            740                 745                 750         


Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn Leu 
        755                 760                 765             


His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys 
    770                 775                 780                 


Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys 
785                 790                 795                 800 


Ser Arg Met Lys Arg Met Ala His Arg Leu Gly Glu Lys Met Leu Asn 
                805                 810                 815     


Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln 
            820                 825                 830         


Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser Asp 
        835                 840                 845             


Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser His 
    850                 855                 860                 


Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe His 
865                 870                 875                 880 


Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe 
                885                 890                 895     


Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro Ile 
            900                 905                 910         


Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile 
        915                 920                 925             


Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln 
    930                 935                 940                 


Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg Val 
945                 950                 955                 960 


Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu Lys 
                965                 970                 975     


Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met Ile 
            980                 985                 990         


His Tyr Gln Ala Val Val Val Leu  Glu Asn Leu Asn Phe  Gly Phe Lys 
        995                 1000                 1005             


Ser Lys  Arg Thr Gly Ile Ala  Glu Lys Ala Val Tyr  Gln Gln Phe 
    1010                 1015                 1020             


Glu Lys  Met Leu Ile Asp Lys  Leu Asn Ser Leu Val  Leu Lys Asp 
    1025                 1030                 1035             


Tyr Pro  Ala Glu Lys Val Gly  Gly Val Leu Asn Pro  Tyr Gln Leu 
    1040                 1045                 1050             


Thr Asp  Gln Phe Thr Ser Phe  Ala Lys Met Gly Thr  Gln Ser Gly 
    1055                 1060                 1065             


Phe Leu  Phe Tyr Val Pro Ala  Pro Tyr Thr Ser Lys  Ile Asp Pro 
    1070                 1075                 1080             


Leu Thr  Gly Phe Val Asp Pro  Phe Val Trp Lys Thr  Ile Lys Asn 
    1085                 1090                 1095             


His Glu  Ser Arg Lys His Phe  Leu Glu Gly Phe Asp  Phe Leu His 
    1100                 1105                 1110             


Tyr Asp  Val Lys Thr Gly Asp  Phe Ile Leu His Phe  Lys Met Asn 
    1115                 1120                 1125             


Arg Asn  Leu Ser Phe Gln Arg  Gly Leu Pro Gly Phe  Met Pro Ala 
    1130                 1135                 1140             


Trp Asp  Ile Val Phe Glu Lys  Asn Glu Thr Gln Phe  Asp Ala Lys 
    1145                 1150                 1155             


Gly Thr  Pro Phe Ile Ala Gly  Lys Arg Ile Val Pro  Val Ile Glu 
    1160                 1165                 1170             


Asn His  Arg Phe Thr Gly Arg  Tyr Arg Asp Leu Tyr  Pro Ala Asn 
    1175                 1180                 1185             


Glu Leu  Ile Ala Leu Leu Glu  Glu Lys Gly Ile Val  Phe Arg Asp 
    1190                 1195                 1200             


Gly Ser  Asn Ile Leu Pro Lys  Leu Leu Glu Asn Asp  Asp Ser His 
    1205                 1210                 1215             


Ala Ile  Asp Thr Met Val Ala  Leu Ile Arg Ser Val  Leu Gln Met 
    1220                 1225                 1230             


Arg Asn  Ser Asn Ala Ala Thr  Gly Glu Asp Tyr Ile  Asn Ser Pro 
    1235                 1240                 1245             


Val Arg  Asp Leu Asn Gly Val  Ser Phe Asp Ser Arg  Phe Gln Asn 
    1250                 1255                 1260             


Pro Glu  Trp Pro Met Asp Ala  Asp Ala Asn Gly Ala  Tyr His Ile 
    1265                 1270                 1275             


Ala Leu  Lys Gly Gln Leu Leu  Leu Asn His Leu Lys  Glu Ser Lys 
    1280                 1285                 1290             


Asp Leu  Lys Leu Gln Asn Gly  Ile Ser Asn Gln Asp  Trp Leu Ala 
    1295                 1300                 1305             


Tyr Ile  Gln Glu Leu Arg Asn  Lys Arg Pro Ala Ala  Thr Lys Lys 
    1310                 1315                 1320             


Ala Gly  Gln Ala Lys Lys Lys  Lys Gly 
    1325                 1330         


<210>  1015
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-nNLS Cys-low


<220>
<221>  MISC_FEATURE
<222>  (3)..(8)
<223>  His tag

<220>
<221>  MISC_FEATURE
<222>  (1316)..(1331)
<223>  Nucleoplasmin NLS

<400>  1015

Met Lys His His His His His His Met Thr Gln Phe Glu Gly Phe Thr 
1               5                   10                  15      


Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln 
            20                  25                  30          


Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp 
        35                  40                  45              


Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg 
    50                  55                  60                  


Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu Asp 
65                  70                  75                  80  


Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr 
                85                  90                  95      


Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn 
            100                 105                 110         


Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala 
        115                 120                 125             


Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu 
    130                 135                 140                 


Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr Thr 
145                 150                 155                 160 


Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr 
                165                 170                 175     


Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu Asp 
            180                 185                 190         


Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro Lys 
        195                 200                 205             


Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val Pro 
    210                 215                 220                 


Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile Phe 
225                 230                 235                 240 


Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln 
                245                 250                 255     


Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly 
            260                 265                 270         


Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu Val 
        275                 280                 285             


Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile Ala 
    290                 295                 300                 


Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp 
305                 310                 315                 320 


Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu 
                325                 330                 335     


Val Ile Gln Ser Phe Ser Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn 
            340                 345                 350         


Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp 
        355                 360                 365             


Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser Ser 
    370                 375                 380                 


Ala Leu Ser Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg 
385                 390                 395                 400 


Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys 
                405                 410                 415     


Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile Ile 
            420                 425                 430         


Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser 
        435                 440                 445             


Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro Thr 
    450                 455                 460                 


Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp 
465                 470                 475                 480 


Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp Glu 
                485                 490                 495     


Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys 
            500                 505                 510         


Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr 
        515                 520                 525             


Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe Gln 
    530                 535                 540                 


Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu Lys Asn Asn 
545                 550                 555                 560 


Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met 
                565                 570                 575     


Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu 
            580                 585                 590         


Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp 
        595                 600                 605             


Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala Val Thr 
    610                 615                 620                 


Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe 
625                 630                 635                 640 


Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro 
                645                 650                 655     


Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly 
            660                 665                 670         


Asp Gln Lys Gly Tyr Arg Glu Ala Leu Ser Lys Trp Ile Asp Phe Thr 
        675                 680                 685             


Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser 
    690                 695                 700                 


Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala 
705                 710                 715                 720 


Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala Glu 
                725                 730                 735     


Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln 
            740                 745                 750         


Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn Leu 
        755                 760                 765             


His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys 
    770                 775                 780                 


Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys 
785                 790                 795                 800 


Ser Arg Met Lys Arg Met Ala His Arg Leu Gly Glu Lys Met Leu Asn 
                805                 810                 815     


Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln 
            820                 825                 830         


Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser Asp 
        835                 840                 845             


Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser His 
    850                 855                 860                 


Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe His 
865                 870                 875                 880 


Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe 
                885                 890                 895     


Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro Ile 
            900                 905                 910         


Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile 
        915                 920                 925             


Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln 
    930                 935                 940                 


Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg Val 
945                 950                 955                 960 


Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu Lys 
                965                 970                 975     


Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met Ile 
            980                 985                 990         


His Tyr Gln Ala Val Val Val Leu  Glu Asn Leu Asn Phe  Gly Phe Lys 
        995                 1000                 1005             


Ser Lys  Arg Thr Gly Ile Ala  Glu Lys Ala Val Tyr  Gln Gln Phe 
    1010                 1015                 1020             


Glu Lys  Met Leu Ile Asp Lys  Leu Asn Cys Leu Val  Leu Lys Asp 
    1025                 1030                 1035             


Tyr Pro  Ala Glu Lys Val Gly  Gly Val Leu Asn Pro  Tyr Gln Leu 
    1040                 1045                 1050             


Thr Asp  Gln Phe Thr Ser Phe  Ala Lys Met Gly Thr  Gln Ser Gly 
    1055                 1060                 1065             


Phe Leu  Phe Tyr Val Pro Ala  Pro Tyr Thr Ser Lys  Ile Asp Pro 
    1070                 1075                 1080             


Leu Thr  Gly Phe Val Asp Pro  Phe Val Trp Lys Thr  Ile Lys Asn 
    1085                 1090                 1095             


His Glu  Ser Arg Lys His Phe  Leu Glu Gly Phe Asp  Phe Leu His 
    1100                 1105                 1110             


Tyr Asp  Val Lys Thr Gly Asp  Phe Ile Leu His Phe  Lys Met Asn 
    1115                 1120                 1125             


Arg Asn  Leu Ser Phe Gln Arg  Gly Leu Pro Gly Phe  Met Pro Ala 
    1130                 1135                 1140             


Trp Asp  Ile Val Phe Glu Lys  Asn Glu Thr Gln Phe  Asp Ala Lys 
    1145                 1150                 1155             


Gly Thr  Pro Phe Ile Ala Gly  Lys Arg Ile Val Pro  Val Ile Glu 
    1160                 1165                 1170             


Asn His  Arg Phe Thr Gly Arg  Tyr Arg Asp Leu Tyr  Pro Ala Asn 
    1175                 1180                 1185             


Glu Leu  Ile Ala Leu Leu Glu  Glu Lys Gly Ile Val  Phe Arg Asp 
    1190                 1195                 1200             


Gly Ser  Asn Ile Leu Pro Lys  Leu Leu Glu Asn Asp  Asp Ser His 
    1205                 1210                 1215             


Ala Ile  Asp Thr Met Val Ala  Leu Ile Arg Ser Val  Leu Gln Met 
    1220                 1225                 1230             


Arg Asn  Ser Asn Ala Ala Thr  Gly Glu Asp Tyr Ile  Asn Ser Pro 
    1235                 1240                 1245             


Val Arg  Asp Leu Asn Gly Val  Cys Phe Asp Ser Arg  Phe Gln Asn 
    1250                 1255                 1260             


Pro Glu  Trp Pro Met Asp Ala  Asp Ala Asn Gly Ala  Tyr His Ile 
    1265                 1270                 1275             


Ala Leu  Lys Gly Gln Leu Leu  Leu Asn His Leu Lys  Glu Ser Lys 
    1280                 1285                 1290             


Asp Leu  Lys Leu Gln Asn Gly  Ile Ser Asn Gln Asp  Trp Leu Ala 
    1295                 1300                 1305             


Tyr Ile  Gln Glu Leu Arg Asn  Lys Arg Pro Ala Ala  Thr Lys Lys 
    1310                 1315                 1320             


Ala Gly  Gln Ala Lys Lys Lys  Lys Gly 
    1325                 1330         


<210>  1016
<211>  1307
<212>  PRT
<213>  Acidaminococcus sp. strain BV3L6

<400>  1016

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn 
    1295                 1300                 1305         


<210>  1017
<211>  1228
<212>  PRT
<213>  Lachnospiraceae bacterium ND2006

<400>  1017

Met Ser Lys Leu Glu Lys Phe Thr Asn Cys Tyr Ser Leu Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Lys Ala Ile Pro Val Gly Lys Thr Gln Glu Asn Ile Asp 
            20                  25                  30          


Asn Lys Arg Leu Leu Val Glu Asp Glu Lys Arg Ala Glu Asp Tyr Lys 
        35                  40                  45              


Gly Val Lys Lys Leu Leu Asp Arg Tyr Tyr Leu Ser Phe Ile Asn Asp 
    50                  55                  60                  


Val Leu His Ser Ile Lys Leu Lys Asn Leu Asn Asn Tyr Ile Ser Leu 
65                  70                  75                  80  


Phe Arg Lys Lys Thr Arg Thr Glu Lys Glu Asn Lys Glu Leu Glu Asn 
                85                  90                  95      


Leu Glu Ile Asn Leu Arg Lys Glu Ile Ala Lys Ala Phe Lys Gly Asn 
            100                 105                 110         


Glu Gly Tyr Lys Ser Leu Phe Lys Lys Asp Ile Ile Glu Thr Ile Leu 
        115                 120                 125             


Pro Glu Phe Leu Asp Asp Lys Asp Glu Ile Ala Leu Val Asn Ser Phe 
    130                 135                 140                 


Asn Gly Phe Thr Thr Ala Phe Thr Gly Phe Phe Asp Asn Arg Glu Asn 
145                 150                 155                 160 


Met Phe Ser Glu Glu Ala Lys Ser Thr Ser Ile Ala Phe Arg Cys Ile 
                165                 170                 175     


Asn Glu Asn Leu Thr Arg Tyr Ile Ser Asn Met Asp Ile Phe Glu Lys 
            180                 185                 190         


Val Asp Ala Ile Phe Asp Lys His Glu Val Gln Glu Ile Lys Glu Lys 
        195                 200                 205             


Ile Leu Asn Ser Asp Tyr Asp Val Glu Asp Phe Phe Glu Gly Glu Phe 
    210                 215                 220                 


Phe Asn Phe Val Leu Thr Gln Glu Gly Ile Asp Val Tyr Asn Ala Ile 
225                 230                 235                 240 


Ile Gly Gly Phe Val Thr Glu Ser Gly Glu Lys Ile Lys Gly Leu Asn 
                245                 250                 255     


Glu Tyr Ile Asn Leu Tyr Asn Gln Lys Thr Lys Gln Lys Leu Pro Lys 
            260                 265                 270         


Phe Lys Pro Leu Tyr Lys Gln Val Leu Ser Asp Arg Glu Ser Leu Ser 
        275                 280                 285             


Phe Tyr Gly Glu Gly Tyr Thr Ser Asp Glu Glu Val Leu Glu Val Phe 
    290                 295                 300                 


Arg Asn Thr Leu Asn Lys Asn Ser Glu Ile Phe Ser Ser Ile Lys Lys 
305                 310                 315                 320 


Leu Glu Lys Leu Phe Lys Asn Phe Asp Glu Tyr Ser Ser Ala Gly Ile 
                325                 330                 335     


Phe Val Lys Asn Gly Pro Ala Ile Ser Thr Ile Ser Lys Asp Ile Phe 
            340                 345                 350         


Gly Glu Trp Asn Val Ile Arg Asp Lys Trp Asn Ala Glu Tyr Asp Asp 
        355                 360                 365             


Ile His Leu Lys Lys Lys Ala Val Val Thr Glu Lys Tyr Glu Asp Asp 
    370                 375                 380                 


Arg Arg Lys Ser Phe Lys Lys Ile Gly Ser Phe Ser Leu Glu Gln Leu 
385                 390                 395                 400 


Gln Glu Tyr Ala Asp Ala Asp Leu Ser Val Val Glu Lys Leu Lys Glu 
                405                 410                 415     


Ile Ile Ile Gln Lys Val Asp Glu Ile Tyr Lys Val Tyr Gly Ser Ser 
            420                 425                 430         


Glu Lys Leu Phe Asp Ala Asp Phe Val Leu Glu Lys Ser Leu Lys Lys 
        435                 440                 445             


Asn Asp Ala Val Val Ala Ile Met Lys Asp Leu Leu Asp Ser Val Lys 
    450                 455                 460                 


Ser Phe Glu Asn Tyr Ile Lys Ala Phe Phe Gly Glu Gly Lys Glu Thr 
465                 470                 475                 480 


Asn Arg Asp Glu Ser Phe Tyr Gly Asp Phe Val Leu Ala Tyr Asp Ile 
                485                 490                 495     


Leu Leu Lys Val Asp His Ile Tyr Asp Ala Ile Arg Asn Tyr Val Thr 
            500                 505                 510         


Gln Lys Pro Tyr Ser Lys Asp Lys Phe Lys Leu Tyr Phe Gln Asn Pro 
        515                 520                 525             


Gln Phe Met Gly Gly Trp Asp Lys Asp Lys Glu Thr Asp Tyr Arg Ala 
    530                 535                 540                 


Thr Ile Leu Arg Tyr Gly Ser Lys Tyr Tyr Leu Ala Ile Met Asp Lys 
545                 550                 555                 560 


Lys Tyr Ala Lys Cys Leu Gln Lys Ile Asp Lys Asp Asp Val Asn Gly 
                565                 570                 575     


Asn Tyr Glu Lys Ile Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys Met 
            580                 585                 590         


Leu Pro Lys Val Phe Phe Ser Lys Lys Trp Met Ala Tyr Tyr Asn Pro 
        595                 600                 605             


Ser Glu Asp Ile Gln Lys Ile Tyr Lys Asn Gly Thr Phe Lys Lys Gly 
    610                 615                 620                 


Asp Met Phe Asn Leu Asn Asp Cys His Lys Leu Ile Asp Phe Phe Lys 
625                 630                 635                 640 


Asp Ser Ile Ser Arg Tyr Pro Lys Trp Ser Asn Ala Tyr Asp Phe Asn 
                645                 650                 655     


Phe Ser Glu Thr Glu Lys Tyr Lys Asp Ile Ala Gly Phe Tyr Arg Glu 
            660                 665                 670         


Val Glu Glu Gln Gly Tyr Lys Val Ser Phe Glu Ser Ala Ser Lys Lys 
        675                 680                 685             


Glu Val Asp Lys Leu Val Glu Glu Gly Lys Leu Tyr Met Phe Gln Ile 
    690                 695                 700                 


Tyr Asn Lys Asp Phe Ser Asp Lys Ser His Gly Thr Pro Asn Leu His 
705                 710                 715                 720 


Thr Met Tyr Phe Lys Leu Leu Phe Asp Glu Asn Asn His Gly Gln Ile 
                725                 730                 735     


Arg Leu Ser Gly Gly Ala Glu Leu Phe Met Arg Arg Ala Ser Leu Lys 
            740                 745                 750         


Lys Glu Glu Leu Val Val His Pro Ala Asn Ser Pro Ile Ala Asn Lys 
        755                 760                 765             


Asn Pro Asp Asn Pro Lys Lys Thr Thr Thr Leu Ser Tyr Asp Val Tyr 
    770                 775                 780                 


Lys Asp Lys Arg Phe Ser Glu Asp Gln Tyr Glu Leu His Ile Pro Ile 
785                 790                 795                 800 


Ala Ile Asn Lys Cys Pro Lys Asn Ile Phe Lys Ile Asn Thr Glu Val 
                805                 810                 815     


Arg Val Leu Leu Lys His Asp Asp Asn Pro Tyr Val Ile Gly Ile Asp 
            820                 825                 830         


Arg Gly Glu Arg Asn Leu Leu Tyr Ile Val Val Val Asp Gly Lys Gly 
        835                 840                 845             


Asn Ile Val Glu Gln Tyr Ser Leu Asn Glu Ile Ile Asn Asn Phe Asn 
    850                 855                 860                 


Gly Ile Arg Ile Lys Thr Asp Tyr His Ser Leu Leu Asp Lys Lys Glu 
865                 870                 875                 880 


Lys Glu Arg Phe Glu Ala Arg Gln Asn Trp Thr Ser Ile Glu Asn Ile 
                885                 890                 895     


Lys Glu Leu Lys Ala Gly Tyr Ile Ser Gln Val Val His Lys Ile Cys 
            900                 905                 910         


Glu Leu Val Glu Lys Tyr Asp Ala Val Ile Ala Leu Glu Asp Leu Asn 
        915                 920                 925             


Ser Gly Phe Lys Asn Ser Arg Val Lys Val Glu Lys Gln Val Tyr Gln 
    930                 935                 940                 


Lys Phe Glu Lys Met Leu Ile Asp Lys Leu Asn Tyr Met Val Asp Lys 
945                 950                 955                 960 


Lys Ser Asn Pro Cys Ala Thr Gly Gly Ala Leu Lys Gly Tyr Gln Ile 
                965                 970                 975     


Thr Asn Lys Phe Glu Ser Phe Lys Ser Met Ser Thr Gln Asn Gly Phe 
            980                 985                 990         


Ile Phe Tyr Ile Pro Ala Trp Leu  Thr Ser Lys Ile Asp  Pro Ser Thr 
        995                 1000                 1005             


Gly Phe  Val Asn Leu Leu Lys  Thr Lys Tyr Thr Ser  Ile Ala Asp 
    1010                 1015                 1020             


Ser Lys  Lys Phe Ile Ser Ser  Phe Asp Arg Ile Met  Tyr Val Pro 
    1025                 1030                 1035             


Glu Glu  Asp Leu Phe Glu Phe  Ala Leu Asp Tyr Lys  Asn Phe Ser 
    1040                 1045                 1050             


Arg Thr  Asp Ala Asp Tyr Ile  Lys Lys Trp Lys Leu  Tyr Ser Tyr 
    1055                 1060                 1065             


Gly Asn  Arg Ile Arg Ile Phe  Arg Asn Pro Lys Lys  Asn Asn Val 
    1070                 1075                 1080             


Phe Asp  Trp Glu Glu Val Cys  Leu Thr Ser Ala Tyr  Lys Glu Leu 
    1085                 1090                 1095             


Phe Asn  Lys Tyr Gly Ile Asn  Tyr Gln Gln Gly Asp  Ile Arg Ala 
    1100                 1105                 1110             


Leu Leu  Cys Glu Gln Ser Asp  Lys Ala Phe Tyr Ser  Ser Phe Met 
    1115                 1120                 1125             


Ala Leu  Met Ser Leu Met Leu  Gln Met Arg Asn Ser  Ile Thr Gly 
    1130                 1135                 1140             


Arg Thr  Asp Val Asp Phe Leu  Ile Ser Pro Val Lys  Asn Ser Asp 
    1145                 1150                 1155             


Gly Ile  Phe Tyr Asp Ser Arg  Asn Tyr Glu Ala Gln  Glu Asn Ala 
    1160                 1165                 1170             


Ile Leu  Pro Lys Asn Ala Asp  Ala Asn Gly Ala Tyr  Asn Ile Ala 
    1175                 1180                 1185             


Arg Lys  Val Leu Trp Ala Ile  Gly Gln Phe Lys Lys  Ala Glu Asp 
    1190                 1195                 1200             


Glu Lys  Leu Asp Lys Val Lys  Ile Ala Ile Ser Asn  Lys Glu Trp 
    1205                 1210                 1215             


Leu Glu  Tyr Ala Gln Thr Ser  Val Lys His 
    1220                 1225             


<210>  1018
<211>  1206
<212>  PRT
<213>  Lachnospiraceae bacterium MA2020

<400>  1018

Met Tyr Tyr Glu Ser Leu Thr Lys Gln Tyr Pro Val Ser Lys Thr Ile 
1               5                   10                  15      


Arg Asn Glu Leu Ile Pro Ile Gly Lys Thr Leu Asp Asn Ile Arg Gln 
            20                  25                  30          


Asn Asn Ile Leu Glu Ser Asp Val Lys Arg Lys Gln Asn Tyr Glu His 
        35                  40                  45              


Val Lys Gly Ile Leu Asp Glu Tyr His Lys Gln Leu Ile Asn Glu Ala 
    50                  55                  60                  


Leu Asp Asn Cys Thr Leu Pro Ser Leu Lys Ile Ala Ala Glu Ile Tyr 
65                  70                  75                  80  


Leu Lys Asn Gln Lys Glu Val Ser Asp Arg Glu Asp Phe Asn Lys Thr 
                85                  90                  95      


Gln Asp Leu Leu Arg Lys Glu Val Val Glu Lys Leu Lys Ala His Glu 
            100                 105                 110         


Asn Phe Thr Lys Ile Gly Lys Lys Asp Ile Leu Asp Leu Leu Glu Lys 
        115                 120                 125             


Leu Pro Ser Ile Ser Glu Asp Asp Tyr Asn Ala Leu Glu Ser Phe Arg 
    130                 135                 140                 


Asn Phe Tyr Thr Tyr Phe Thr Ser Tyr Asn Lys Val Arg Glu Asn Leu 
145                 150                 155                 160 


Tyr Ser Asp Lys Glu Lys Ser Ser Thr Val Ala Tyr Arg Leu Ile Asn 
                165                 170                 175     


Glu Asn Phe Pro Lys Phe Leu Asp Asn Val Lys Ser Tyr Arg Phe Val 
            180                 185                 190         


Lys Thr Ala Gly Ile Leu Ala Asp Gly Leu Gly Glu Glu Glu Gln Asp 
        195                 200                 205             


Ser Leu Phe Ile Val Glu Thr Phe Asn Lys Thr Leu Thr Gln Asp Gly 
    210                 215                 220                 


Ile Asp Thr Tyr Asn Ser Gln Val Gly Lys Ile Asn Ser Ser Ile Asn 
225                 230                 235                 240 


Leu Tyr Asn Gln Lys Asn Gln Lys Ala Asn Gly Phe Arg Lys Ile Pro 
                245                 250                 255     


Lys Met Lys Met Leu Tyr Lys Gln Ile Leu Ser Asp Arg Glu Glu Ser 
            260                 265                 270         


Phe Ile Asp Glu Phe Gln Ser Asp Glu Val Leu Ile Asp Asn Val Glu 
        275                 280                 285             


Ser Tyr Gly Ser Val Leu Ile Glu Ser Leu Lys Ser Ser Lys Val Ser 
    290                 295                 300                 


Ala Phe Phe Asp Ala Leu Arg Glu Ser Lys Gly Lys Asn Val Tyr Val 
305                 310                 315                 320 


Lys Asn Asp Leu Ala Lys Thr Ala Met Ser Asn Ile Val Phe Glu Asn 
                325                 330                 335     


Trp Arg Thr Phe Asp Asp Leu Leu Asn Gln Glu Tyr Asp Leu Ala Asn 
            340                 345                 350         


Glu Asn Lys Lys Lys Asp Asp Lys Tyr Phe Glu Lys Arg Gln Lys Glu 
        355                 360                 365             


Leu Lys Lys Asn Lys Ser Tyr Ser Leu Glu His Leu Cys Asn Leu Ser 
    370                 375                 380                 


Glu Asp Ser Cys Asn Leu Ile Glu Asn Tyr Ile His Gln Ile Ser Asp 
385                 390                 395                 400 


Asp Ile Glu Asn Ile Ile Ile Asn Asn Glu Thr Phe Leu Arg Ile Val 
                405                 410                 415     


Ile Asn Glu His Asp Arg Ser Arg Lys Leu Ala Lys Asn Arg Lys Ala 
            420                 425                 430         


Val Lys Ala Ile Lys Asp Phe Leu Asp Ser Ile Lys Val Leu Glu Arg 
        435                 440                 445             


Glu Leu Lys Leu Ile Asn Ser Ser Gly Gln Glu Leu Glu Lys Asp Leu 
    450                 455                 460                 


Ile Val Tyr Ser Ala His Glu Glu Leu Leu Val Glu Leu Lys Gln Val 
465                 470                 475                 480 


Asp Ser Leu Tyr Asn Met Thr Arg Asn Tyr Leu Thr Lys Lys Pro Phe 
                485                 490                 495     


Ser Thr Glu Lys Val Lys Leu Asn Phe Asn Arg Ser Thr Leu Leu Asn 
            500                 505                 510         


Gly Trp Asp Arg Asn Lys Glu Thr Asp Asn Leu Gly Val Leu Leu Leu 
        515                 520                 525             


Lys Asp Gly Lys Tyr Tyr Leu Gly Ile Met Asn Thr Ser Ala Asn Lys 
    530                 535                 540                 


Ala Phe Val Asn Pro Pro Val Ala Lys Thr Glu Lys Val Phe Lys Lys 
545                 550                 555                 560 


Val Asp Tyr Lys Leu Leu Pro Val Pro Asn Gln Met Leu Pro Lys Val 
                565                 570                 575     


Phe Phe Ala Lys Ser Asn Ile Asp Phe Tyr Asn Pro Ser Ser Glu Ile 
            580                 585                 590         


Tyr Ser Asn Tyr Lys Lys Gly Thr His Lys Lys Gly Asn Met Phe Ser 
        595                 600                 605             


Leu Glu Asp Cys His Asn Leu Ile Asp Phe Phe Lys Glu Ser Ile Ser 
    610                 615                 620                 


Lys His Glu Asp Trp Ser Lys Phe Gly Phe Lys Phe Ser Asp Thr Ala 
625                 630                 635                 640 


Ser Tyr Asn Asp Ile Ser Glu Phe Tyr Arg Glu Val Glu Lys Gln Gly 
                645                 650                 655     


Tyr Lys Leu Thr Tyr Thr Asp Ile Asp Glu Thr Tyr Ile Asn Asp Leu 
            660                 665                 670         


Ile Glu Arg Asn Glu Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe 
        675                 680                 685             


Ser Met Tyr Ser Lys Gly Lys Leu Asn Leu His Thr Leu Tyr Phe Met 
    690                 695                 700                 


Met Leu Phe Asp Gln Arg Asn Ile Asp Asp Val Val Tyr Lys Leu Asn 
705                 710                 715                 720 


Gly Glu Ala Glu Val Phe Tyr Arg Pro Ala Ser Ile Ser Glu Asp Glu 
                725                 730                 735     


Leu Ile Ile His Lys Ala Gly Glu Glu Ile Lys Asn Lys Asn Pro Asn 
            740                 745                 750         


Arg Ala Arg Thr Lys Glu Thr Ser Thr Phe Ser Tyr Asp Ile Val Lys 
        755                 760                 765             


Asp Lys Arg Tyr Ser Lys Asp Lys Phe Thr Leu His Ile Pro Ile Thr 
    770                 775                 780                 


Met Asn Phe Gly Val Asp Glu Val Lys Arg Phe Asn Asp Ala Val Asn 
785                 790                 795                 800 


Ser Ala Ile Arg Ile Asp Glu Asn Val Asn Val Ile Gly Ile Asp Arg 
                805                 810                 815     


Gly Glu Arg Asn Leu Leu Tyr Val Val Val Ile Asp Ser Lys Gly Asn 
            820                 825                 830         


Ile Leu Glu Gln Ile Ser Leu Asn Ser Ile Ile Asn Lys Glu Tyr Asp 
        835                 840                 845             


Ile Glu Thr Asp Tyr His Ala Leu Leu Asp Glu Arg Glu Gly Gly Arg 
    850                 855                 860                 


Asp Lys Ala Arg Lys Asp Trp Asn Thr Val Glu Asn Ile Arg Asp Leu 
865                 870                 875                 880 


Lys Ala Gly Tyr Leu Ser Gln Val Val Asn Val Val Ala Lys Leu Val 
                885                 890                 895     


Leu Lys Tyr Asn Ala Ile Ile Cys Leu Glu Asp Leu Asn Phe Gly Phe 
            900                 905                 910         


Lys Arg Gly Arg Gln Lys Val Glu Lys Gln Val Tyr Gln Lys Phe Glu 
        915                 920                 925             


Lys Met Leu Ile Asp Lys Leu Asn Tyr Leu Val Ile Asp Lys Ser Arg 
    930                 935                 940                 


Glu Gln Thr Ser Pro Lys Glu Leu Gly Gly Ala Leu Asn Ala Leu Gln 
945                 950                 955                 960 


Leu Thr Ser Lys Phe Lys Ser Phe Lys Glu Leu Gly Lys Gln Ser Gly 
                965                 970                 975     


Val Ile Tyr Tyr Val Pro Ala Tyr Leu Thr Ser Lys Ile Asp Pro Thr 
            980                 985                 990         


Thr Gly Phe Ala Asn Leu Phe Tyr  Met Lys Cys Glu Asn  Val Glu Lys 
        995                 1000                 1005             


Ser Lys  Arg Phe Phe Asp Gly  Phe Asp Phe Ile Arg  Phe Asn Ala 
    1010                 1015                 1020             


Leu Glu  Asn Val Phe Glu Phe  Gly Phe Asp Tyr Arg  Ser Phe Thr 
    1025                 1030                 1035             


Gln Arg  Ala Cys Gly Ile Asn  Ser Lys Trp Thr Val  Cys Thr Asn 
    1040                 1045                 1050             


Gly Glu  Arg Ile Ile Lys Tyr  Arg Asn Pro Asp Lys  Asn Asn Met 
    1055                 1060                 1065             


Phe Asp  Glu Lys Val Val Val  Val Thr Asp Glu Met  Lys Asn Leu 
    1070                 1075                 1080             


Phe Glu  Gln Tyr Lys Ile Pro  Tyr Glu Asp Gly Arg  Asn Val Lys 
    1085                 1090                 1095             


Asp Met  Ile Ile Ser Asn Glu  Glu Ala Glu Phe Tyr  Arg Arg Leu 
    1100                 1105                 1110             


Tyr Arg  Leu Leu Gln Gln Thr  Leu Gln Met Arg Asn  Ser Thr Ser 
    1115                 1120                 1125             


Asp Gly  Thr Arg Asp Tyr Ile  Ile Ser Pro Val Lys  Asn Lys Arg 
    1130                 1135                 1140             


Glu Ala  Tyr Phe Asn Ser Glu  Leu Ser Asp Gly Ser  Val Pro Lys 
    1145                 1150                 1155             


Asp Ala  Asp Ala Asn Gly Ala  Tyr Asn Ile Ala Arg  Lys Gly Leu 
    1160                 1165                 1170             


Trp Val  Leu Glu Gln Ile Arg  Gln Lys Ser Glu Gly  Glu Lys Ile 
    1175                 1180                 1185             


Asn Leu  Ala Met Thr Asn Ala  Glu Trp Leu Glu Tyr  Ala Gln Thr 
    1190                 1195                 1200             


His Leu  Leu 
    1205     


<210>  1019
<211>  4059
<212>  DNA
<213>  Acidaminococcus sp. strain BV3L6

<400>  1019
atgacccagt tcgagggctt caccaacctg taccaggtga gcaagaccct gcggttcgag       60

ctgatccccc agggcaagac cctgaagcac atccaggagc agggcttcat cgaggaggac      120

aaggcccgga acgaccacta caaggagctg aagcccatca tcgaccggat ctacaagacc      180

tacgccgacc agtgcctgca gctggtgcag ctggactggg agaacctgag cgccgccatc      240

gacagctacc ggaaggagaa gaccgaggag acccggaacg ccctgatcga ggagcaggcc      300

acctaccgga acgccatcca cgactacttc atcggccgga ccgacaacct gaccgacgcc      360

atcaacaagc ggcacgccga gatctacaag ggcctgttca aggccgagct gttcaacggc      420

aaggtgctga agcagctggg caccgtgacc accaccgagc acgagaacgc cctgctgcgg      480

agcttcgaca agttcaccac ctacttcagc ggcttctacg agaaccggaa gaacgtgttc      540

agcgccgagg acatcagcac cgccatcccc caccggatcg tgcaggacaa cttccccaag      600

ttcaaggaga actgccacat cttcacccgg ctgatcaccg ccgtgcccag cctgcgggag      660

cacttcgaga acgtgaagaa ggccatcggc atcttcgtga gcaccagcat cgaggaggtg      720

ttcagcttcc ccttctacaa ccagctgctg acccagaccc agatcgacct gtacaaccag      780

ctgctgggcg gcatcagccg ggaggccggc accgagaaga tcaagggcct gaacgaggtg      840

ctgaacctgg ccatccagaa gaacgacgag accgcccaca tcatcgccag cctgccccac      900

cggttcatcc ccctgttcaa gcagatcctg agcgaccgga acaccctgag cttcatcctg      960

gaggagttca agagcgacga ggaggtgatc cagagcttct gcaagtacaa gaccctgctg     1020

cggaacgaga acgtgctgga gaccgccgag gccctgttca acgagctgaa cagcatcgac     1080

ctgacccaca tcttcatcag ccacaagaag ctggagacca tcagcagcgc cctgtgcgac     1140

cactgggaca ccctgcggaa cgccctgtac gagcggcgga tcagcgagct gaccggcaag     1200

atcaccaaga gcgccaagga gaaggtgcag cggagcctga agcacgagga catcaacctg     1260

caggagatca tcagcgccgc cggcaaggag ctgagcgagg ccttcaagca gaagaccagc     1320

gagatcctga gccacgccca cgccgccctg gaccagcccc tgcccaccac cctgaagaag     1380

caggaggaga aggagatcct gaaaagccag ctggacagcc tgctgggcct gtaccacctg     1440

ctggactggt tcgccgtgga cgagagcaac gaggtggacc ccgagttcag cgcccggctg     1500

accggcatca agctggagat ggagcccagc ctgagcttct acaacaaggc ccggaactac     1560

gccaccaaga agccctacag cgtggagaag ttcaagctga acttccagat gcccaccctg     1620

gccagcggct gggacgtgaa caaggagaag aacaacggcg ccatcctgtt cgtgaagaac     1680

ggcctgtact acctgggcat catgcccaag cagaagggcc ggtacaaggc cctgagcttc     1740

gagcccaccg agaagaccag cgagggcttc gacaagatgt actacgacta cttccccgac     1800

gccgccaaga tgatccccaa gtgcagcacc cagctgaagg ccgtgaccgc ccacttccag     1860

acccacacca cccccatcct gctgagcaac aacttcatcg agcccctgga gatcaccaag     1920

gagatctacg acctgaacaa ccccgagaag gagcccaaga agttccagac cgcctacgcc     1980

aagaagaccg gcgaccagaa gggctaccgg gaggccctgt gcaagtggat cgacttcacc     2040

cgggacttcc tgagcaagta caccaagacc accagcatcg acctgagcag cctgcggccc     2100

agcagccagt acaaggacct gggcgagtac tacgccgagc tgaaccccct gctgtaccac     2160

atcagcttcc agcggatcgc cgagaaggag atcatggacg ccgtggagac cggcaagctg     2220

tacctgttcc agatctacaa caaggacttc gccaagggcc accacggcaa gcccaacctg     2280

cacaccctgt actggaccgg cctgttcagc cccgagaacc tggccaagac cagcatcaag     2340

ctgaacggcc aggccgagct gttctaccgg cccaagagcc ggatgaagcg gatggcccac     2400

cggctgggcg agaagatgct gaacaagaag ctgaaggacc agaagacccc catccccgac     2460

accctgtacc aggagctgta cgactacgtg aaccaccggc tgagccacga cctgagcgac     2520

gaggcccggg ccctgctgcc caacgtgatc accaaggagg tgagccacga gatcatcaag     2580

gaccggcggt tcaccagcga caagttcttc ttccacgtgc ccatcaccct gaactaccag     2640

gccgccaaca gccccagcaa gttcaaccag cgggtgaacg cctacctgaa ggagcacccc     2700

gagaccccca tcatcggcat cgaccggggc gagcggaacc tgatctacat caccgtgatc     2760

gacagcaccg gcaagatcct ggagcagcgg agcctgaaca ccatccagca gttcgactac     2820

cagaagaagc tggacaaccg ggagaaggag cgggtggccg cccggcaggc ctggagcgtg     2880

gtgggcacca tcaaggacct gaagcagggc tacctgagcc aggtgatcca cgagatcgtg     2940

gacctgatga tccactacca ggccgtggtg gtgctggaga acctgaactt cggcttcaag     3000

agcaagcgga ccggcatcgc cgagaaggcc gtgtaccagc agttcgagaa gatgctgatc     3060

gacaagctga actgcctggt gctgaaggac taccccgccg agaaggtggg cggcgtgctg     3120

aacccctacc agctgaccga ccagttcacc agcttcgcca agatgggcac ccagagcggc     3180

ttcctgttct acgtgcccgc cccctacacc agcaagatcg accccctgac cggcttcgtg     3240

gaccccttcg tgtggaagac catcaagaac cacgagagcc ggaagcactt cctggagggc     3300

ttcgacttcc tgcactacga cgtgaagacc ggcgacttca tcctgcactt caagatgaac     3360

cggaacctga gcttccagcg gggcctgccc ggcttcatgc ccgcctggga catcgtgttc     3420

gagaagaacg agacccagtt cgacgccaag ggcaccccct tcatcgccgg caagcggatc     3480

gtgcccgtga tcgagaacca ccggttcacc ggccggtacc gggacctgta ccccgccaac     3540

gagctgatcg ccctgctgga ggagaagggc atcgtgttcc gggacggcag caacatcctg     3600

cccaagctgc tggagaacga cgacagccac gccatcgaca ccatggtggc cctgatccgg     3660

agcgtgctgc agatgcggaa cagcaacgcc gccaccggcg aggactacat caacagcccc     3720

gtgcgggacc tgaacggcgt gtgcttcgac agccggttcc agaaccccga gtggcccatg     3780

gacgccgacg ccaacggcgc ctaccacatc gccctgaagg gccagctgct gctgaaccac     3840

ctgaaggaga gcaaggacct gaagctgcag aacggcatca gcaaccagga ctggctggcc     3900

tacatccagg agctgcggaa caagcggccc gccgccacca agaaggccgg ccaggccaag     3960

aagaagaagg gcagctaccc ctacgacgtg cccgactacg cctaccccta cgacgtgccc     4020

gactacgcct acccctacga cgtgcccgac tacgcctga                            4059


<210>  1020
<211>  3822
<212>  DNA
<213>  Lachnospiraceae bacterium ND2006

<400>  1020
atgagcaagc tggagaagtt caccaactgc tacagcctga gcaagaccct gcggttcaag       60

gccatccccg tgggcaagac ccaggagaac atcgacaaca agcggctgct ggtggaggac      120

gagaagcggg ccgaggacta caagggcgtg aagaagctgc tggaccggta ctacctgagc      180

ttcatcaacg acgtgctgca cagcatcaag ctgaagaacc tgaacaacta catcagcctg      240

ttccggaaga agacccggac cgagaaggag aacaaggagc tggagaacct ggagatcaac      300

ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg gctacaagag cctgttcaag      360

aaggacatca tcgagaccat cctgcccgag ttcctggacg acaaggacga gatcgccctg      420

gtgaacagct tcaacggctt caccaccgcc ttcaccggct tcttcgacaa ccgggagaac      480

atgttcagcg aggaggccaa gagcaccagc atcgccttcc ggtgcatcaa cgagaacctg      540

acccggtaca tcagcaacat ggacatcttc gagaaggtgg acgccatctt cgacaagcac      600

gaggtgcagg agatcaagga gaagatcctg aacagcgact acgacgtgga ggacttcttc      660

gagggcgagt tcttcaactt cgtgctgacc caggagggca tcgacgtgta caacgccatc      720

atcggcggct tcgtgaccga gagcggcgag aagatcaagg gcctgaacga gtacatcaac      780

ctgtacaacc agaagaccaa gcagaagctg cccaagttca agcccctgta caagcaggtg      840

ctgagcgacc gggagagcct gagcttctac ggcgagggct acaccagcga cgaggaggtg      900

ctggaggtgt tccggaacac cctgaacaag aacagcgaga tcttcagcag catcaagaag      960

ctggagaagc tgttcaagaa cttcgacgag tacagcagcg ccggcatctt cgtgaagaac     1020

ggccccgcca tcagcaccat cagcaaggac atcttcggcg agtggaacgt gatccgggac     1080

aagtggaacg ccgagtacga cgacatccac ctgaagaaga aggccgtggt gaccgagaag     1140

tacgaggacg accggcggaa aagcttcaag aagatcggca gcttcagcct ggagcagctg     1200

caggagtacg ccgacgccga cctgagcgtg gtggagaagc tgaaggagat catcatccag     1260

aaggtggacg agatctacaa ggtgtacggc agcagcgaga agctgttcga cgccgacttc     1320

gtgctggaga aaagcctgaa gaagaacgac gccgtggtgg ccatcatgaa ggacctgctg     1380

gacagcgtga aaagcttcga gaactacatc aaggccttct tcggcgaggg caaggagacc     1440

aaccgggacg agagcttcta cggcgacttc gtgctggcct acgacatcct gctgaaggtg     1500

gaccacatct acgacgccat ccggaactac gtgacccaga agccctacag caaggacaag     1560

ttcaagctgt acttccagaa cccccagttc atgggcggct gggacaagga caaggagacc     1620

gactaccggg ccaccatcct gcggtacggc agcaagtact acctggccat catggacaag     1680

aagtacgcca agtgcctgca gaagatcgac aaggacgacg tgaacggcaa ctacgagaag     1740

atcaactaca agctgctgcc cggccccaac aagatgctgc ccaaggtgtt cttcagcaag     1800

aagtggatgg cctactacaa ccccagcgag gacatccaga agatctacaa gaacggcacc     1860

ttcaagaagg gcgacatgtt caacctgaac gactgccaca agctgatcga cttcttcaag     1920

gacagcatca gccggtaccc caagtggagc aacgcctacg acttcaactt cagcgagacc     1980

gagaagtaca aggacatcgc cggcttctac cgggaggtgg aggagcaggg ctacaaggtg     2040

agcttcgaga gcgccagcaa gaaggaggtg gacaagctgg tggaggaggg caagctgtac     2100

atgttccaga tctacaacaa ggacttcagc gacaagagcc acggcacccc caacctgcac     2160

accatgtact tcaagctgct gttcgacgag aacaaccacg gccagatccg gctgagcggc     2220

ggcgccgagc tgttcatgcg gcgggccagc ctgaagaagg aggagctggt ggtgcacccc     2280

gccaacagcc ccatcgccaa caagaacccc gacaacccca agaagaccac caccctgagc     2340

tacgacgtgt acaaggacaa gcggttcagc gaggaccagt acgagctgca catccccatc     2400

gccatcaaca agtgccccaa gaacatcttc aagatcaaca ccgaggtgcg ggtgctgctg     2460

aagcacgacg acaaccccta cgtgatcggc atcgaccggg gcgagcggaa cctgctgtac     2520

atcgtggtgg tggacggcaa gggcaacatc gtggagcagt acagcctgaa cgagatcatc     2580

aacaacttca acggcatccg gatcaagacc gactaccaca gcctgctgga caagaaggag     2640

aaggagcggt tcgaggcccg gcagaactgg accagcatcg agaacatcaa ggagctgaag     2700

gccggctaca tcagccaggt ggtgcacaag atctgcgagc tggtggagaa gtacgacgcc     2760

gtgatcgccc tggaggacct gaacagcggc ttcaagaaca gccgggtgaa ggtggagaag     2820

caggtgtacc agaagttcga gaagatgctg atcgacaagc tgaactacat ggtggacaag     2880

aaaagcaacc cctgcgccac cggcggcgcc ctgaagggct accagatcac caacaagttc     2940

gagagcttca agagcatgag cacccagaac ggcttcatct tctacatccc cgcctggctg     3000

accagcaaga tcgaccccag caccggcttc gtgaacctgc tgaagaccaa gtacaccagc     3060

atcgccgaca gcaagaagtt catcagcagc ttcgaccgga tcatgtacgt gcccgaggag     3120

gacctgttcg agttcgccct ggactacaag aacttcagcc ggaccgacgc cgactacatc     3180

aagaagtgga agctgtacag ctacggcaac cggatccgga tcttccggaa ccccaagaag     3240

aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg cctacaagga gctgttcaac     3300

aagtacggca tcaactacca gcagggcgac atccgggccc tgctgtgcga gcagagcgac     3360

aaggccttct acagcagctt catggccctg atgagcctga tgctgcagat gcggaacagc     3420

atcaccggcc ggaccgacgt ggacttcctg atcagccccg tgaagaacag cgacggcatc     3480

ttctacgaca gccggaacta cgaggcccag gagaacgcca tcctgcccaa gaacgccgac     3540

gccaacggcg cctacaacat cgcccggaag gtgctgtggg ccatcggcca gttcaagaag     3600

gccgaggacg agaagctgga caaggtgaag atcgccatca gcaacaagga gtggctggag     3660

tacgcccaga ccagcgtgaa gcacaagcgg cccgccgcca ccaagaaggc cggccaggcc     3720

aagaagaaga agggcagcta cccctacgac gtgcccgact acgcctaccc ctacgacgtg     3780

cccgactacg cctaccccta cgacgtgccc gactacgcct ga                        3822


<210>  1021
<211>  3756
<212>  DNA
<213>  Lachnospiraceae bacterium MA2020

<400>  1021
atgtactacg agagcctgac caagcagtac cccgtgagca agaccatccg gaacgagctg       60

atccccatcg gcaagaccct ggacaacatc cggcagaaca acatcctgga gagcgacgtg      120

aagcggaagc agaactacga gcacgtgaag ggcatcctgg acgagtacca caagcagctg      180

atcaacgagg ccctggacaa ctgcaccctg cccagcctga agatcgccgc cgagatctac      240

ctgaagaacc agaaggaggt gagcgaccgg gaggacttca acaagaccca ggacctgctg      300

cggaaggagg tggtggagaa gctgaaggcc cacgagaact tcaccaagat cggcaagaag      360

gacatcctgg acctgctgga gaagctgccc agcatcagcg aggacgacta caacgccctg      420

gagagcttcc ggaacttcta cacctacttc accagctaca acaaggtgcg ggagaacctg      480

tacagcgaca aggagaaaag cagcaccgtg gcctaccggc tgatcaacga gaacttcccc      540

aagttcctgg acaacgtgaa aagctaccgg ttcgtgaaga ccgccggcat cctggccgac      600

ggcctgggcg aggaggagca ggacagcctg ttcatcgtgg agaccttcaa caagaccctg      660

acccaggacg gcatcgacac ctacaacagc caggtgggca agatcaacag cagcatcaac      720

ctgtacaacc agaagaacca gaaggccaac ggcttccgga agatccccaa gatgaagatg      780

ctgtacaagc agatcctgag cgaccgggag gagagcttca tcgacgagtt ccagagcgac      840

gaggtgctga tcgacaacgt ggagagctac ggcagcgtgc tgatcgagag cctgaaaagc      900

agcaaggtga gcgccttctt cgacgccctg cgggagagca agggcaagaa cgtgtacgtg      960

aagaacgacc tggccaagac cgccatgagc aacatcgtgt tcgagaactg gcggaccttc     1020

gacgacctgc tgaaccagga gtacgacctg gccaacgaga acaagaagaa ggacgacaag     1080

tacttcgaga agcggcagaa ggagctgaag aagaacaaga gctacagcct ggagcacctg     1140

tgcaacctga gcgaggacag ctgcaacctg atcgagaact acatccacca gatcagcgac     1200

gacatcgaga acatcatcat caacaacgag accttcctgc ggatcgtgat caacgagcac     1260

gaccggagcc ggaagctggc caagaaccgg aaggccgtga aggccatcaa ggacttcctg     1320

gacagcatca aggtgctgga gcgggagctg aagctgatca acagcagcgg ccaggagctg     1380

gagaaggacc tgatcgtgta cagcgcccac gaggagctgc tggtggagct gaagcaggtg     1440

gacagcctgt acaacatgac ccggaactac ctgaccaaga agcccttcag caccgagaag     1500

gtgaagctga acttcaaccg gagcaccctg ctgaacggct gggaccggaa caaggagacc     1560

gacaacctgg gcgtgctgct gctgaaggac ggcaagtact acctgggcat catgaacacc     1620

agcgccaaca aggccttcgt gaaccccccc gtggccaaga ccgagaaggt gttcaagaag     1680

gtggactaca agctgctgcc cgtgcccaac cagatgctgc ccaaggtgtt cttcgccaag     1740

agcaacatcg acttctacaa ccccagcagc gagatctaca gcaactacaa gaagggcacc     1800

cacaagaagg gcaacatgtt cagcctggag gactgccaca acctgatcga cttcttcaag     1860

gagagcatca gcaagcacga ggactggagc aagttcggct tcaagttcag cgacaccgcc     1920

agctacaacg acatcagcga gttctaccgg gaggtggaga agcagggcta caagctgacc     1980

tacaccgaca tcgacgagac ctacatcaac gacctgatcg agcggaacga gctgtacctg     2040

ttccagatct acaacaagga cttcagcatg tacagcaagg gcaagctgaa cctgcacacc     2100

ctgtacttca tgatgctgtt cgaccagcgg aacatcgacg acgtggtgta caagctgaac     2160

ggcgaggccg aggtgttcta ccggcccgcc agcatcagcg aggacgagct gatcatccac     2220

aaggccggcg aggagatcaa gaacaagaac cccaaccggg cccggaccaa ggagaccagc     2280

accttcagct acgacatcgt gaaggacaag cggtacagca aggacaagtt caccctgcac     2340

atccccatca ccatgaactt cggcgtggac gaggtgaagc ggttcaacga cgccgtgaac     2400

agcgccatcc ggatcgacga gaacgtgaac gtgatcggca tcgaccgggg cgagcggaac     2460

ctgctgtacg tggtggtgat cgacagcaag ggcaacatcc tggagcagat cagcctgaac     2520

agcatcatca acaaggagta cgacatcgag accgactacc acgccctgct ggacgagcgg     2580

gagggcggcc gggacaaggc ccggaaggac tggaacaccg tggagaacat ccgggacctg     2640

aaggccggct acctgagcca ggtggtgaac gtggtggcca agctggtgct gaagtacaac     2700

gccatcatct gcctggagga cctgaacttc ggcttcaagc ggggccggca gaaggtggag     2760

aagcaggtgt accagaagtt cgagaagatg ctgatcgaca agctgaacta cctggtgatc     2820

gacaagagcc gggagcagac cagccccaag gagctgggcg gcgccctgaa cgccctgcag     2880

ctgaccagca agttcaagag cttcaaggag ctgggcaagc agagcggcgt gatctactac     2940

gtgcccgcct acctgaccag caagatcgac cccaccaccg gcttcgccaa cctgttctac     3000

atgaagtgcg agaacgtgga gaaaagcaag cggttcttcg acggcttcga cttcatccgg     3060

ttcaacgccc tggagaacgt gttcgagttc ggcttcgact accggagctt cacccagcgg     3120

gcctgcggca tcaacagcaa gtggaccgtg tgcaccaacg gcgagcggat catcaagtac     3180

cggaaccccg acaagaacaa catgttcgac gagaaggtgg tggtggtgac cgacgagatg     3240

aagaacctgt tcgagcagta caagatcccc tacgaggacg gccggaacgt gaaggacatg     3300

atcatcagca acgaggaggc cgagttctac cggcggctgt accggctgct gcagcagacc     3360

ctgcagatgc ggaacagcac cagcgacggc acccgggact acatcatcag ccccgtgaag     3420

aacaagcggg aggcctactt caacagcgag ctgagcgacg gcagcgtgcc caaggacgcc     3480

gacgccaacg gcgcctacaa catcgcccgg aagggcctgt gggtgctgga gcagatccgg     3540

cagaaaagcg agggcgagaa gatcaacctg gccatgacca acgccgagtg gctggagtac     3600

gcccagaccc acctgctgaa gcggcccgcc gccaccaaga aggccggcca ggccaagaag     3660

aagaagggca gctaccccta cgacgtgccc gactacgcct acccctacga cgtgcccgac     3720

tacgcctacc cctacgacgt gcccgactac gcctga                               3756


<210>  1022
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 full-length gRNA

<400>  1022
uaauuucuac ucuuguagau ccuugucaag gcuauugguc                             40


<210>  1023
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 modified full-length gRNA


<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  Modified to include 2'-O-methyl

<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  Linked by phosphorodithioate (PS2) bond

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Modified to include 2'-O-methyl

<400>  1023
uaauuucuac ucuuguagau ccuugucaag gcuauugguc a                           41


<210>  1024
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tSpA dead gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (92)..(93)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (92)..(92)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (93)..(94)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (93)..(93)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (94)..(95)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (94)..(94)
<223>  Modified to contain 2'-O-methyl

<400>  1024
ggcuggccaa cccauguuuu agagcuagaa auagcaaguu aaaauaaggc uaguccguua       60

ucaacuugaa aaaguggcac cgagucggug cuuuu                                  95


<210>  1025
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SpA full-length gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (97)..(98)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (98)..(98)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (98)..(99)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(99)
<223>  Modified to contain 2'-O-methyl

<400>  1025
ggcaaggcug gccaacccau guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  1026
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  SpG full-length gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (97)..(98)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (98)..(98)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (98)..(99)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(99)
<223>  Modified to contain 2'-O-methyl

<400>  1026
uagucuuaga guauccagug guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcuuuu                            100


<210>  1027
<211>  95
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Sp182 dead gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (92)..(93)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (92)..(92)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (93)..(94)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (93)..(93)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (94)..(95)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (94)..(94)
<223>  Modified to contain 2'-O-methyl

<400>  1027
uuagaguauc cagugguuuu agagcuagaa auagcaaguu aaaauaaggc uaguccguua       60

ucaacuugaa aaaguggcac cgagucggug cuuuu                                  95


<210>  1028
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Sp182 dead gRNA targeting domain

<400>  1028
uuagaguauc cagug                                                        15


<210>  1029
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sp182 dgRNA targeting domain

<400>  1029
ttagagtatc cagtg                                                        15


<210>  1030
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SpG gRNA targeting domain

<400>  1030
tagtcttaga gtatccagtg                                                   20


<210>  1031
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  tSpA dead gRNA targeting domain

<400>  1031
ggctggccaa cccat                                                        15


<210>  1032
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-sNLS-sNLS H800A


<220>
<221>  MISC_FEATURE
<222>  (3)..(8)
<223>  His tag

<220>
<221>  MISC_FEATURE
<222>  (1319)..(1325)
<223>  sNLS

<220>
<221>  MISC_FEATURE
<222>  (1328)..(1334)
<223>  sNLS

<400>  1032

Met Gly His His His His His His Gly Ser Thr Gln Phe Glu Gly Phe 
1               5                   10                  15      


Thr Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro 
            20                  25                  30          


Gln Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu 
        35                  40                  45              


Asp Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp 
    50                  55                  60                  


Arg Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu 
65                  70                  75                  80  


Asp Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys 
                85                  90                  95      


Thr Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg 
            100                 105                 110         


Asn Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp 
        115                 120                 125             


Ala Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala 
    130                 135                 140                 


Glu Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr 
145                 150                 155                 160 


Thr Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr 
                165                 170                 175     


Tyr Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu 
            180                 185                 190         


Asp Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro 
        195                 200                 205             


Lys Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val 
    210                 215                 220                 


Pro Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile 
225                 230                 235                 240 


Phe Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn 
                245                 250                 255     


Gln Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly 
            260                 265                 270         


Gly Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu 
        275                 280                 285             


Val Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile 
    290                 295                 300                 


Ala Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser 
305                 310                 315                 320 


Asp Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu 
                325                 330                 335     


Glu Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu 
            340                 345                 350         


Asn Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile 
        355                 360                 365             


Asp Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser 
    370                 375                 380                 


Ser Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu 
385                 390                 395                 400 


Arg Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu 
                405                 410                 415     


Lys Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile 
            420                 425                 430         


Ile Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr 
        435                 440                 445             


Ser Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro 
    450                 455                 460                 


Thr Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu 
465                 470                 475                 480 


Asp Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp 
                485                 490                 495     


Glu Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile 
            500                 505                 510         


Lys Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn 
        515                 520                 525             


Tyr Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe 
    530                 535                 540                 


Gln Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu Lys Asn 
545                 550                 555                 560 


Asn Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile 
                565                 570                 575     


Met Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr 
            580                 585                 590         


Glu Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro 
        595                 600                 605             


Asp Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala Val 
    610                 615                 620                 


Thr Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn 
625                 630                 635                 640 


Phe Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn 
                645                 650                 655     


Pro Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr 
            660                 665                 670         


Gly Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe 
        675                 680                 685             


Thr Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu 
    690                 695                 700                 


Ser Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr 
705                 710                 715                 720 


Ala Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala 
                725                 730                 735     


Glu Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe 
            740                 745                 750         


Gln Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn 
        755                 760                 765             


Leu His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala 
    770                 775                 780                 


Lys Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro 
785                 790                 795                 800 


Lys Ser Arg Met Lys Arg Met Ala Ala Arg Leu Gly Glu Lys Met Leu 
                805                 810                 815     


Asn Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr 
            820                 825                 830         


Gln Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser 
        835                 840                 845             


Asp Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser 
    850                 855                 860                 


His Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys 
                885                 890                 895     


Phe Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro 
            900                 905                 910         


Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val 
        915                 920                 925             


Ile Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile 
    930                 935                 940                 


Gln Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg 
945                 950                 955                 960 


Val Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu 
                965                 970                 975     


Lys Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met 
            980                 985                 990         


Ile His Tyr Gln Ala Val Val Val  Leu Glu Asn Leu Asn  Phe Gly Phe 
        995                 1000                 1005             


Lys Ser  Lys Arg Thr Gly Ile  Ala Glu Lys Ala Val  Tyr Gln Gln 
    1010                 1015                 1020             


Phe Glu  Lys Met Leu Ile Asp  Lys Leu Asn Cys Leu  Val Leu Lys 
    1025                 1030                 1035             


Asp Tyr  Pro Ala Glu Lys Val  Gly Gly Val Leu Asn  Pro Tyr Gln 
    1040                 1045                 1050             


Leu Thr  Asp Gln Phe Thr Ser  Phe Ala Lys Met Gly  Thr Gln Ser 
    1055                 1060                 1065             


Gly Phe  Leu Phe Tyr Val Pro  Ala Pro Tyr Thr Ser  Lys Ile Asp 
    1070                 1075                 1080             


Pro Leu  Thr Gly Phe Val Asp  Pro Phe Val Trp Lys  Thr Ile Lys 
    1085                 1090                 1095             


Asn His  Glu Ser Arg Lys His  Phe Leu Glu Gly Phe  Asp Phe Leu 
    1100                 1105                 1110             


His Tyr  Asp Val Lys Thr Gly  Asp Phe Ile Leu His  Phe Lys Met 
    1115                 1120                 1125             


Asn Arg  Asn Leu Ser Phe Gln  Arg Gly Leu Pro Gly  Phe Met Pro 
    1130                 1135                 1140             


Ala Trp  Asp Ile Val Phe Glu  Lys Asn Glu Thr Gln  Phe Asp Ala 
    1145                 1150                 1155             


Lys Gly  Thr Pro Phe Ile Ala  Gly Lys Arg Ile Val  Pro Val Ile 
    1160                 1165                 1170             


Glu Asn  His Arg Phe Thr Gly  Arg Tyr Arg Asp Leu  Tyr Pro Ala 
    1175                 1180                 1185             


Asn Glu  Leu Ile Ala Leu Leu  Glu Glu Lys Gly Ile  Val Phe Arg 
    1190                 1195                 1200             


Asp Gly  Ser Asn Ile Leu Pro  Lys Leu Leu Glu Asn  Asp Asp Ser 
    1205                 1210                 1215             


His Ala  Ile Asp Thr Met Val  Ala Leu Ile Arg Ser  Val Leu Gln 
    1220                 1225                 1230             


Met Arg  Asn Ser Asn Ala Ala  Thr Gly Glu Asp Tyr  Ile Asn Ser 
    1235                 1240                 1245             


Pro Val  Arg Asp Leu Asn Gly  Val Cys Phe Asp Ser  Arg Phe Gln 
    1250                 1255                 1260             


Asn Pro  Glu Trp Pro Met Asp  Ala Asp Ala Asn Gly  Ala Tyr His 
    1265                 1270                 1275             


Ile Ala  Leu Lys Gly Gln Leu  Leu Leu Asn His Leu  Lys Glu Ser 
    1280                 1285                 1290             


Lys Asp  Leu Lys Leu Gln Asn  Gly Ile Ser Asn Gln  Asp Trp Leu 
    1295                 1300                 1305             


Ala Tyr  Ile Gln Glu Leu Arg  Asn Gly Ser Pro Lys  Lys Lys Arg 
    1310                 1315                 1320             


Lys Val  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1325                 1330                 


<210>  1033
<211>  1384
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SpCas9WT

<400>  1033

Met Lys His His His His His His Met Pro Lys Lys Lys Arg Lys Val 
1               5                   10                  15      


Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
            20                  25                  30          


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
        35                  40                  45              


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
    50                  55                  60                  


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
65                  70                  75                  80  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
                85                  90                  95      


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
            100                 105                 110         


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
        115                 120                 125             


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
    130                 135                 140                 


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
145                 150                 155                 160 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
                165                 170                 175     


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
            180                 185                 190         


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
        195                 200                 205             


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
    210                 215                 220                 


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
225                 230                 235                 240 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
                245                 250                 255     


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
            260                 265                 270         


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
        275                 280                 285             


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
    290                 295                 300                 


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
305                 310                 315                 320 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
                325                 330                 335     


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
            340                 345                 350         


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
        355                 360                 365             


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
    370                 375                 380                 


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
385                 390                 395                 400 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
                405                 410                 415     


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
            420                 425                 430         


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
        435                 440                 445             


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
465                 470                 475                 480 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
                485                 490                 495     


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
            500                 505                 510         


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
        515                 520                 525             


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
    530                 535                 540                 


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
545                 550                 555                 560 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
                565                 570                 575     


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
            580                 585                 590         


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
        595                 600                 605             


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
    610                 615                 620                 


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
625                 630                 635                 640 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
                645                 650                 655     


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
            660                 665                 670         


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
        675                 680                 685             


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
    690                 695                 700                 


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
705                 710                 715                 720 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
                725                 730                 735     


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
            740                 745                 750         


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
        755                 760                 765             


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
    770                 775                 780                 


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
785                 790                 795                 800 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
                805                 810                 815     


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
            820                 825                 830         


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
        835                 840                 845             


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
    850                 855                 860                 


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
865                 870                 875                 880 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
                885                 890                 895     


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
            900                 905                 910         


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
        915                 920                 925             


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
    930                 935                 940                 


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
945                 950                 955                 960 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
                965                 970                 975     


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
            980                 985                 990         


Glu Ile Asn Asn Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ala Val 
        995                 1000                 1005             


Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu 
    1010                 1015                 1020             


Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile 
    1025                 1030                 1035             


Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe 
    1040                 1045                 1050             


Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu 
    1055                 1060                 1065             


Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly 
    1070                 1075                 1080             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr 
    1085                 1090                 1095             


Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys Lys 
    1100                 1105                 1110             


Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro 
    1115                 1120                 1125             


Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp 
    1130                 1135                 1140             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser 
    1145                 1150                 1155             


Val Leu  Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu 
    1160                 1165                 1170             


Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1175                 1180                 1185             


Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr 
    1190                 1195                 1200             


Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser 
    1205                 1210                 1215             


Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala 
    1220                 1225                 1230             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr 
    1235                 1240                 1245             


Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly 
    1250                 1255                 1260             


Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu Phe Val  Glu Gln His 
    1265                 1270                 1275             


Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser 
    1280                 1285                 1290             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser 
    1295                 1300                 1305             


Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu 
    1310                 1315                 1320             


Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala 
    1325                 1330                 1335             


Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr 
    1340                 1345                 1350             


Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
    1355                 1360                 1365             


Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly 
    1370                 1375                 1380             


Asp 
    


<210>  1034
<211>  1384
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-NLS-SpCas9D10A

<400>  1034

Met Lys His His His His His His Met Pro Lys Lys Lys Arg Lys Val 
1               5                   10                  15      


Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
            20                  25                  30          


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
        35                  40                  45              


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
    50                  55                  60                  


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
65                  70                  75                  80  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
                85                  90                  95      


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
            100                 105                 110         


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
        115                 120                 125             


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
    130                 135                 140                 


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
145                 150                 155                 160 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
                165                 170                 175     


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
            180                 185                 190         


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
        195                 200                 205             


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
    210                 215                 220                 


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
225                 230                 235                 240 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
                245                 250                 255     


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
            260                 265                 270         


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
        275                 280                 285             


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
    290                 295                 300                 


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
305                 310                 315                 320 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
                325                 330                 335     


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
            340                 345                 350         


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
        355                 360                 365             


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
    370                 375                 380                 


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
385                 390                 395                 400 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
                405                 410                 415     


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
            420                 425                 430         


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
        435                 440                 445             


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
    450                 455                 460                 


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
465                 470                 475                 480 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
                485                 490                 495     


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
            500                 505                 510         


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
        515                 520                 525             


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
    530                 535                 540                 


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
545                 550                 555                 560 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
                565                 570                 575     


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
            580                 585                 590         


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
        595                 600                 605             


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
    610                 615                 620                 


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
625                 630                 635                 640 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
                645                 650                 655     


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
            660                 665                 670         


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
        675                 680                 685             


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
    690                 695                 700                 


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
705                 710                 715                 720 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
                725                 730                 735     


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
            740                 745                 750         


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
        755                 760                 765             


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
    770                 775                 780                 


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
785                 790                 795                 800 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
                805                 810                 815     


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
            820                 825                 830         


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
        835                 840                 845             


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
    850                 855                 860                 


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
865                 870                 875                 880 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
                885                 890                 895     


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
            900                 905                 910         


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
        915                 920                 925             


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
    930                 935                 940                 


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
945                 950                 955                 960 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
                965                 970                 975     


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
            980                 985                 990         


Glu Ile Asn Asn Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ala Val 
        995                 1000                 1005             


Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu 
    1010                 1015                 1020             


Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile 
    1025                 1030                 1035             


Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe 
    1040                 1045                 1050             


Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu 
    1055                 1060                 1065             


Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly 
    1070                 1075                 1080             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr 
    1085                 1090                 1095             


Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys Lys 
    1100                 1105                 1110             


Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro 
    1115                 1120                 1125             


Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp 
    1130                 1135                 1140             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser 
    1145                 1150                 1155             


Val Leu  Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu 
    1160                 1165                 1170             


Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1175                 1180                 1185             


Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr 
    1190                 1195                 1200             


Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser 
    1205                 1210                 1215             


Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala 
    1220                 1225                 1230             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr 
    1235                 1240                 1245             


Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly 
    1250                 1255                 1260             


Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu Phe Val  Glu Gln His 
    1265                 1270                 1275             


Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser 
    1280                 1285                 1290             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser 
    1295                 1300                 1305             


Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu 
    1310                 1315                 1320             


Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala 
    1325                 1330                 1335             


Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr 
    1340                 1345                 1350             


Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
    1355                 1360                 1365             


Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly 
    1370                 1375                 1380             


Asp 
    


<210>  1035
<211>  1333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AsCpf1 His-AsCpf1-NLS-NLS

<400>  1035

Met Lys His His His His His His Met Thr Gln Phe Glu Gly Phe Thr 
1               5                   10                  15      


Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln 
            20                  25                  30          


Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp 
        35                  40                  45              


Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg 
    50                  55                  60                  


Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu Asp 
65                  70                  75                  80  


Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr 
                85                  90                  95      


Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn 
            100                 105                 110         


Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala 
        115                 120                 125             


Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu 
    130                 135                 140                 


Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr Thr 
145                 150                 155                 160 


Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr 
                165                 170                 175     


Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu Asp 
            180                 185                 190         


Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro Lys 
        195                 200                 205             


Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val Pro 
    210                 215                 220                 


Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile Phe 
225                 230                 235                 240 


Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln 
                245                 250                 255     


Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly 
            260                 265                 270         


Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu Val 
        275                 280                 285             


Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile Ala 
    290                 295                 300                 


Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp 
305                 310                 315                 320 


Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu 
                325                 330                 335     


Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn 
            340                 345                 350         


Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp 
        355                 360                 365             


Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser Ser 
    370                 375                 380                 


Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg 
385                 390                 395                 400 


Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys 
                405                 410                 415     


Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile Ile 
            420                 425                 430         


Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser 
        435                 440                 445             


Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro Thr 
    450                 455                 460                 


Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp 
465                 470                 475                 480 


Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp Glu 
                485                 490                 495     


Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys 
            500                 505                 510         


Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr 
        515                 520                 525             


Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe Gln 
    530                 535                 540                 


Met Pro Thr Leu Ala Ser Gly Trp Asp Val Asn Lys Glu Lys Asn Asn 
545                 550                 555                 560 


Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met 
                565                 570                 575     


Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu 
            580                 585                 590         


Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp 
        595                 600                 605             


Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala Val Thr 
    610                 615                 620                 


Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe 
625                 630                 635                 640 


Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro 
                645                 650                 655     


Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly 
            660                 665                 670         


Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe Thr 
        675                 680                 685             


Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser 
    690                 695                 700                 


Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala 
705                 710                 715                 720 


Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala Glu 
                725                 730                 735     


Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln 
            740                 745                 750         


Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn Leu 
        755                 760                 765             


His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys 
    770                 775                 780                 


Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys 
785                 790                 795                 800 


Ser Arg Met Lys Arg Met Ala His Arg Leu Gly Glu Lys Met Leu Asn 
                805                 810                 815     


Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln 
            820                 825                 830         


Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser Asp 
        835                 840                 845             


Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser His 
    850                 855                 860                 


Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe His 
865                 870                 875                 880 


Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe 
                885                 890                 895     


Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro Ile 
            900                 905                 910         


Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile 
        915                 920                 925             


Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln 
    930                 935                 940                 


Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg Val 
945                 950                 955                 960 


Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu Lys 
                965                 970                 975     


Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met Ile 
            980                 985                 990         


His Tyr Gln Ala Val Val Val Leu  Glu Asn Leu Asn Phe  Gly Phe Lys 
        995                 1000                 1005             


Ser Lys  Arg Thr Gly Ile Ala  Glu Lys Ala Val Tyr  Gln Gln Phe 
    1010                 1015                 1020             


Glu Lys  Met Leu Ile Asp Lys  Leu Asn Cys Leu Val  Leu Lys Asp 
    1025                 1030                 1035             


Tyr Pro  Ala Glu Lys Val Gly  Gly Val Leu Asn Pro  Tyr Gln Leu 
    1040                 1045                 1050             


Thr Asp  Gln Phe Thr Ser Phe  Ala Lys Met Gly Thr  Gln Ser Gly 
    1055                 1060                 1065             


Phe Leu  Phe Tyr Val Pro Ala  Pro Tyr Thr Ser Lys  Ile Asp Pro 
    1070                 1075                 1080             


Leu Thr  Gly Phe Val Asp Pro  Phe Val Trp Lys Thr  Ile Lys Asn 
    1085                 1090                 1095             


His Glu  Ser Arg Lys His Phe  Leu Glu Gly Phe Asp  Phe Leu His 
    1100                 1105                 1110             


Tyr Asp  Val Lys Thr Gly Asp  Phe Ile Leu His Phe  Lys Met Asn 
    1115                 1120                 1125             


Arg Asn  Leu Ser Phe Gln Arg  Gly Leu Pro Gly Phe  Met Pro Ala 
    1130                 1135                 1140             


Trp Asp  Ile Val Phe Glu Lys  Asn Glu Thr Gln Phe  Asp Ala Lys 
    1145                 1150                 1155             


Gly Thr  Pro Phe Ile Ala Gly  Lys Arg Ile Val Pro  Val Ile Glu 
    1160                 1165                 1170             


Asn His  Arg Phe Thr Gly Arg  Tyr Arg Asp Leu Tyr  Pro Ala Asn 
    1175                 1180                 1185             


Glu Leu  Ile Ala Leu Leu Glu  Glu Lys Gly Ile Val  Phe Arg Asp 
    1190                 1195                 1200             


Gly Ser  Asn Ile Leu Pro Lys  Leu Leu Glu Asn Asp  Asp Ser His 
    1205                 1210                 1215             


Ala Ile  Asp Thr Met Val Ala  Leu Ile Arg Ser Val  Leu Gln Met 
    1220                 1225                 1230             


Arg Asn  Ser Asn Ala Ala Thr  Gly Glu Asp Tyr Ile  Asn Ser Pro 
    1235                 1240                 1245             


Val Arg  Asp Leu Asn Gly Val  Cys Phe Asp Ser Arg  Phe Gln Asn 
    1250                 1255                 1260             


Pro Glu  Trp Pro Met Asp Ala  Asp Ala Asn Gly Ala  Tyr His Ile 
    1265                 1270                 1275             


Ala Leu  Lys Gly Gln Leu Leu  Leu Asn His Leu Lys  Glu Ser Lys 
    1280                 1285                 1290             


Asp Leu  Lys Leu Gln Asn Gly  Ile Ser Asn Gln Asp  Trp Leu Ala 
    1295                 1300                 1305             


Tyr Ile  Gln Glu Leu Arg Asn  Gly Ser Pro Lys Lys  Lys Arg Lys 
    1310                 1315                 1320             


Val Gly  Ser Pro Lys Lys Lys  Arg Lys Val 
    1325                 1330             


<210>  1036
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AsCpf1 S542R/K607R  (AsCpf1 RR)

<400>  1036

Met Lys His His His His His His Met Pro Lys Lys Lys Arg Lys Val 
1               5                   10                  15      


Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
            20                  25                  30          


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
        35                  40                  45              


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
    50                  55                  60                  


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
65                  70                  75                  80  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
                85                  90                  95      


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
            100                 105                 110         


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
        115                 120                 125             


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
    130                 135                 140                 


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
145                 150                 155                 160 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
                165                 170                 175     


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
            180                 185                 190         


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
        195                 200                 205             


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
    210                 215                 220                 


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
225                 230                 235                 240 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
                245                 250                 255     


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
            260                 265                 270         


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
        275                 280                 285             


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
    290                 295                 300                 


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
305                 310                 315                 320 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
                325                 330                 335     


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
            340                 345                 350         


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
        355                 360                 365             


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
    370                 375                 380                 


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
385                 390                 395                 400 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
                405                 410                 415     


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
            420                 425                 430         


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
        435                 440                 445             


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
    450                 455                 460                 


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
465                 470                 475                 480 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
                485                 490                 495     


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
            500                 505                 510         


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
        515                 520                 525             


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
    530                 535                 540                 


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Arg Gly Trp 
545                 550                 555                 560 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
                565                 570                 575     


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
            580                 585                 590         


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
        595                 600                 605             


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Arg Cys 
    610                 615                 620                 


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
625                 630                 635                 640 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
                645                 650                 655     


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
            660                 665                 670         


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
        675                 680                 685             


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
    690                 695                 700                 


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
705                 710                 715                 720 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
                725                 730                 735     


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
            740                 745                 750         


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
        755                 760                 765             


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
    770                 775                 780                 


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
785                 790                 795                 800 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
                805                 810                 815     


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
            820                 825                 830         


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
        835                 840                 845             


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
    850                 855                 860                 


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
865                 870                 875                 880 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
                885                 890                 895     


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
            900                 905                 910         


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
        915                 920                 925             


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
    930                 935                 940                 


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
945                 950                 955                 960 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
                965                 970                 975     


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
            980                 985                 990         


His Glu Ile Val Asp Leu Met Ile  His Tyr Gln Ala Val  Val Val Leu 
        995                 1000                 1005             


Glu Asn  Leu Asn Phe Gly Phe  Lys Ser Lys Arg Thr  Gly Ile Ala 
    1010                 1015                 1020             


Glu Lys  Ala Val Tyr Gln Gln  Phe Glu Lys Met Leu  Ile Asp Lys 
    1025                 1030                 1035             


Leu Asn  Cys Leu Val Leu Lys  Asp Tyr Pro Ala Glu  Lys Val Gly 
    1040                 1045                 1050             


Gly Val  Leu Asn Pro Tyr Gln  Leu Thr Asp Gln Phe  Thr Ser Phe 
    1055                 1060                 1065             


Ala Lys  Met Gly Thr Gln Ser  Gly Phe Leu Phe Tyr  Val Pro Ala 
    1070                 1075                 1080             


Pro Tyr  Thr Ser Lys Ile Asp  Pro Leu Thr Gly Phe  Val Asp Pro 
    1085                 1090                 1095             


Phe Val  Trp Lys Thr Ile Lys  Asn His Glu Ser Arg  Lys His Phe 
    1100                 1105                 1110             


Leu Glu  Gly Phe Asp Phe Leu  His Tyr Asp Val Lys  Thr Gly Asp 
    1115                 1120                 1125             


Phe Ile  Leu His Phe Lys Met  Asn Arg Asn Leu Ser  Phe Gln Arg 
    1130                 1135                 1140             


Gly Leu  Pro Gly Phe Met Pro  Ala Trp Asp Ile Val  Phe Glu Lys 
    1145                 1150                 1155             


Asn Glu  Thr Gln Phe Asp Ala  Lys Gly Thr Pro Phe  Ile Ala Gly 
    1160                 1165                 1170             


Lys Arg  Ile Val Pro Val Ile  Glu Asn His Arg Phe  Thr Gly Arg 
    1175                 1180                 1185             


Tyr Arg  Asp Leu Tyr Pro Ala  Asn Glu Leu Ile Ala  Leu Leu Glu 
    1190                 1195                 1200             


Glu Lys  Gly Ile Val Phe Arg  Asp Gly Ser Asn Ile  Leu Pro Lys 
    1205                 1210                 1215             


Leu Leu  Glu Asn Asp Asp Ser  His Ala Ile Asp Thr  Met Val Ala 
    1220                 1225                 1230             


Leu Ile  Arg Ser Val Leu Gln  Met Arg Asn Ser Asn  Ala Ala Thr 
    1235                 1240                 1245             


Gly Glu  Asp Tyr Ile Asn Ser  Pro Val Arg Asp Leu  Asn Gly Val 
    1250                 1255                 1260             


Cys Phe  Asp Ser Arg Phe Gln  Asn Pro Glu Trp Pro  Met Asp Ala 
    1265                 1270                 1275             


Asp Ala  Asn Gly Ala Tyr His  Ile Ala Leu Lys Gly  Gln Leu Leu 
    1280                 1285                 1290             


Leu Asn  His Leu Lys Glu Ser  Lys Asp Leu Lys Leu  Gln Asn Gly 
    1295                 1300                 1305             


Ile Ser  Asn Gln Asp Trp Leu  Ala Tyr Ile Gln Glu  Leu Arg Asn 
    1310                 1315                 1320             


Gly Pro  Lys Lys Lys Arg Lys  Val Gly 
    1325                 1330         


<210>  1037
<211>  1332
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AsCpf1 S542R/K548V/N552R (AsCpf1 RVR)

<400>  1037

Met Lys His His His His His His Met Pro Lys Lys Lys Arg Lys Val 
1               5                   10                  15      


Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
            20                  25                  30          


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
        35                  40                  45              


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
    50                  55                  60                  


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
65                  70                  75                  80  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
                85                  90                  95      


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
            100                 105                 110         


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
        115                 120                 125             


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
    130                 135                 140                 


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
145                 150                 155                 160 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
                165                 170                 175     


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
            180                 185                 190         


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
        195                 200                 205             


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
    210                 215                 220                 


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
225                 230                 235                 240 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
                245                 250                 255     


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
            260                 265                 270         


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
        275                 280                 285             


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
    290                 295                 300                 


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
305                 310                 315                 320 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
                325                 330                 335     


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
            340                 345                 350         


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
        355                 360                 365             


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
    370                 375                 380                 


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
385                 390                 395                 400 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
                405                 410                 415     


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
            420                 425                 430         


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
        435                 440                 445             


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
    450                 455                 460                 


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
465                 470                 475                 480 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
                485                 490                 495     


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
            500                 505                 510         


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
        515                 520                 525             


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
    530                 535                 540                 


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Arg Gly Trp 
545                 550                 555                 560 


Asp Val Asn Val Glu Lys Asn Arg Gly Ala Ile Leu Phe Val Lys Asn 
                565                 570                 575     


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
            580                 585                 590         


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
        595                 600                 605             


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
    610                 615                 620                 


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
625                 630                 635                 640 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
                645                 650                 655     


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
            660                 665                 670         


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
        675                 680                 685             


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
    690                 695                 700                 


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
705                 710                 715                 720 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
                725                 730                 735     


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
            740                 745                 750         


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
        755                 760                 765             


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
    770                 775                 780                 


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
785                 790                 795                 800 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
                805                 810                 815     


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
            820                 825                 830         


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
        835                 840                 845             


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
    850                 855                 860                 


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
865                 870                 875                 880 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
                885                 890                 895     


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
            900                 905                 910         


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
        915                 920                 925             


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
    930                 935                 940                 


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
945                 950                 955                 960 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
                965                 970                 975     


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
            980                 985                 990         


His Glu Ile Val Asp Leu Met Ile  His Tyr Gln Ala Val  Val Val Leu 
        995                 1000                 1005             


Glu Asn  Leu Asn Phe Gly Phe  Lys Ser Lys Arg Thr  Gly Ile Ala 
    1010                 1015                 1020             


Glu Lys  Ala Val Tyr Gln Gln  Phe Glu Lys Met Leu  Ile Asp Lys 
    1025                 1030                 1035             


Leu Asn  Cys Leu Val Leu Lys  Asp Tyr Pro Ala Glu  Lys Val Gly 
    1040                 1045                 1050             


Gly Val  Leu Asn Pro Tyr Gln  Leu Thr Asp Gln Phe  Thr Ser Phe 
    1055                 1060                 1065             


Ala Lys  Met Gly Thr Gln Ser  Gly Phe Leu Phe Tyr  Val Pro Ala 
    1070                 1075                 1080             


Pro Tyr  Thr Ser Lys Ile Asp  Pro Leu Thr Gly Phe  Val Asp Pro 
    1085                 1090                 1095             


Phe Val  Trp Lys Thr Ile Lys  Asn His Glu Ser Arg  Lys His Phe 
    1100                 1105                 1110             


Leu Glu  Gly Phe Asp Phe Leu  His Tyr Asp Val Lys  Thr Gly Asp 
    1115                 1120                 1125             


Phe Ile  Leu His Phe Lys Met  Asn Arg Asn Leu Ser  Phe Gln Arg 
    1130                 1135                 1140             


Gly Leu  Pro Gly Phe Met Pro  Ala Trp Asp Ile Val  Phe Glu Lys 
    1145                 1150                 1155             


Asn Glu  Thr Gln Phe Asp Ala  Lys Gly Thr Pro Phe  Ile Ala Gly 
    1160                 1165                 1170             


Lys Arg  Ile Val Pro Val Ile  Glu Asn His Arg Phe  Thr Gly Arg 
    1175                 1180                 1185             


Tyr Arg  Asp Leu Tyr Pro Ala  Asn Glu Leu Ile Ala  Leu Leu Glu 
    1190                 1195                 1200             


Glu Lys  Gly Ile Val Phe Arg  Asp Gly Ser Asn Ile  Leu Pro Lys 
    1205                 1210                 1215             


Leu Leu  Glu Asn Asp Asp Ser  His Ala Ile Asp Thr  Met Val Ala 
    1220                 1225                 1230             


Leu Ile  Arg Ser Val Leu Gln  Met Arg Asn Ser Asn  Ala Ala Thr 
    1235                 1240                 1245             


Gly Glu  Asp Tyr Ile Asn Ser  Pro Val Arg Asp Leu  Asn Gly Val 
    1250                 1255                 1260             


Cys Phe  Asp Ser Arg Phe Gln  Asn Pro Glu Trp Pro  Met Asp Ala 
    1265                 1270                 1275             


Asp Ala  Asn Gly Ala Tyr His  Ile Ala Leu Lys Gly  Gln Leu Leu 
    1280                 1285                 1290             


Leu Asn  His Leu Lys Glu Ser  Lys Asp Leu Lys Leu  Gln Asn Gly 
    1295                 1300                 1305             


Ile Ser  Asn Gln Asp Trp Leu  Ala Tyr Ile Gln Glu  Leu Arg Asn 
    1310                 1315                 1320             


Gly Pro  Lys Lys Lys Arg Lys  Val Gly 
    1325                 1330         


<210>  1038
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-2xNLS-S542R/K607R/H800A (AsCpf1 RR-H800A)

<400>  1038

Met Gly His His His His His His Gly Ser Thr Gln Phe Glu Gly Phe 
1               5                   10                  15      


Thr Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro 
            20                  25                  30          


Gln Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu 
        35                  40                  45              


Asp Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp 
    50                  55                  60                  


Arg Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu 
65                  70                  75                  80  


Asp Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys 
                85                  90                  95      


Thr Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg 
            100                 105                 110         


Asn Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp 
        115                 120                 125             


Ala Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala 
    130                 135                 140                 


Glu Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr 
145                 150                 155                 160 


Thr Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr 
                165                 170                 175     


Tyr Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu 
            180                 185                 190         


Asp Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro 
        195                 200                 205             


Lys Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val 
    210                 215                 220                 


Pro Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile 
225                 230                 235                 240 


Phe Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn 
                245                 250                 255     


Gln Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly 
            260                 265                 270         


Gly Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu 
        275                 280                 285             


Val Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile 
    290                 295                 300                 


Ala Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser 
305                 310                 315                 320 


Asp Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu 
                325                 330                 335     


Glu Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu 
            340                 345                 350         


Asn Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile 
        355                 360                 365             


Asp Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser 
    370                 375                 380                 


Ser Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu 
385                 390                 395                 400 


Arg Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu 
                405                 410                 415     


Lys Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile 
            420                 425                 430         


Ile Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr 
        435                 440                 445             


Ser Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro 
    450                 455                 460                 


Thr Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu 
465                 470                 475                 480 


Asp Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp 
                485                 490                 495     


Glu Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile 
            500                 505                 510         


Lys Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn 
        515                 520                 525             


Tyr Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe 
    530                 535                 540                 


Gln Met Pro Thr Leu Ala Arg Gly Trp Asp Val Asn Lys Glu Lys Asn 
545                 550                 555                 560 


Asn Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile 
                565                 570                 575     


Met Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr 
            580                 585                 590         


Glu Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro 
        595                 600                 605             


Asp Ala Ala Lys Met Ile Pro Arg Cys Ser Thr Gln Leu Lys Ala Val 
    610                 615                 620                 


Thr Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn 
625                 630                 635                 640 


Phe Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn 
                645                 650                 655     


Pro Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr 
            660                 665                 670         


Gly Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe 
        675                 680                 685             


Thr Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu 
    690                 695                 700                 


Ser Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr 
705                 710                 715                 720 


Ala Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala 
                725                 730                 735     


Glu Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe 
            740                 745                 750         


Gln Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn 
        755                 760                 765             


Leu His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala 
    770                 775                 780                 


Lys Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro 
785                 790                 795                 800 


Lys Ser Arg Met Lys Arg Met Ala Ala Arg Leu Gly Glu Lys Met Leu 
                805                 810                 815     


Asn Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr 
            820                 825                 830         


Gln Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser 
        835                 840                 845             


Asp Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser 
    850                 855                 860                 


His Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys 
                885                 890                 895     


Phe Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro 
            900                 905                 910         


Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val 
        915                 920                 925             


Ile Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile 
    930                 935                 940                 


Gln Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg 
945                 950                 955                 960 


Val Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu 
                965                 970                 975     


Lys Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met 
            980                 985                 990         


Ile His Tyr Gln Ala Val Val Val  Leu Glu Asn Leu Asn  Phe Gly Phe 
        995                 1000                 1005             


Lys Ser  Lys Arg Thr Gly Ile  Ala Glu Lys Ala Val  Tyr Gln Gln 
    1010                 1015                 1020             


Phe Glu  Lys Met Leu Ile Asp  Lys Leu Asn Cys Leu  Val Leu Lys 
    1025                 1030                 1035             


Asp Tyr  Pro Ala Glu Lys Val  Gly Gly Val Leu Asn  Pro Tyr Gln 
    1040                 1045                 1050             


Leu Thr  Asp Gln Phe Thr Ser  Phe Ala Lys Met Gly  Thr Gln Ser 
    1055                 1060                 1065             


Gly Phe  Leu Phe Tyr Val Pro  Ala Pro Tyr Thr Ser  Lys Ile Asp 
    1070                 1075                 1080             


Pro Leu  Thr Gly Phe Val Asp  Pro Phe Val Trp Lys  Thr Ile Lys 
    1085                 1090                 1095             


Asn His  Glu Ser Arg Lys His  Phe Leu Glu Gly Phe  Asp Phe Leu 
    1100                 1105                 1110             


His Tyr  Asp Val Lys Thr Gly  Asp Phe Ile Leu His  Phe Lys Met 
    1115                 1120                 1125             


Asn Arg  Asn Leu Ser Phe Gln  Arg Gly Leu Pro Gly  Phe Met Pro 
    1130                 1135                 1140             


Ala Trp  Asp Ile Val Phe Glu  Lys Asn Glu Thr Gln  Phe Asp Ala 
    1145                 1150                 1155             


Lys Gly  Thr Pro Phe Ile Ala  Gly Lys Arg Ile Val  Pro Val Ile 
    1160                 1165                 1170             


Glu Asn  His Arg Phe Thr Gly  Arg Tyr Arg Asp Leu  Tyr Pro Ala 
    1175                 1180                 1185             


Asn Glu  Leu Ile Ala Leu Leu  Glu Glu Lys Gly Ile  Val Phe Arg 
    1190                 1195                 1200             


Asp Gly  Ser Asn Ile Leu Pro  Lys Leu Leu Glu Asn  Asp Asp Ser 
    1205                 1210                 1215             


His Ala  Ile Asp Thr Met Val  Ala Leu Ile Arg Ser  Val Leu Gln 
    1220                 1225                 1230             


Met Arg  Asn Ser Asn Ala Ala  Thr Gly Glu Asp Tyr  Ile Asn Ser 
    1235                 1240                 1245             


Pro Val  Arg Asp Leu Asn Gly  Val Cys Phe Asp Ser  Arg Phe Gln 
    1250                 1255                 1260             


Asn Pro  Glu Trp Pro Met Asp  Ala Asp Ala Asn Gly  Ala Tyr His 
    1265                 1270                 1275             


Ile Ala  Leu Lys Gly Gln Leu  Leu Leu Asn His Leu  Lys Glu Ser 
    1280                 1285                 1290             


Lys Asp  Leu Lys Leu Gln Asn  Gly Ile Ser Asn Gln  Asp Trp Leu 
    1295                 1300                 1305             


Ala Tyr  Ile Gln Glu Leu Arg  Asn Gly Ser Pro Lys  Lys Lys Arg 
    1310                 1315                 1320             


Lys Val  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1325                 1330                 


<210>  1039
<211>  1334
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-2xNLS-S542R-K548V-N552R-H800A (Cpf1 RVR H800A)

<400>  1039

Met Gly His His His His His His Gly Ser Thr Gln Phe Glu Gly Phe 
1               5                   10                  15      


Thr Asn Leu Tyr Gln Val Ser Lys Thr Leu Arg Phe Glu Leu Ile Pro 
            20                  25                  30          


Gln Gly Lys Thr Leu Lys His Ile Gln Glu Gln Gly Phe Ile Glu Glu 
        35                  40                  45              


Asp Lys Ala Arg Asn Asp His Tyr Lys Glu Leu Lys Pro Ile Ile Asp 
    50                  55                  60                  


Arg Ile Tyr Lys Thr Tyr Ala Asp Gln Cys Leu Gln Leu Val Gln Leu 
65                  70                  75                  80  


Asp Trp Glu Asn Leu Ser Ala Ala Ile Asp Ser Tyr Arg Lys Glu Lys 
                85                  90                  95      


Thr Glu Glu Thr Arg Asn Ala Leu Ile Glu Glu Gln Ala Thr Tyr Arg 
            100                 105                 110         


Asn Ala Ile His Asp Tyr Phe Ile Gly Arg Thr Asp Asn Leu Thr Asp 
        115                 120                 125             


Ala Ile Asn Lys Arg His Ala Glu Ile Tyr Lys Gly Leu Phe Lys Ala 
    130                 135                 140                 


Glu Leu Phe Asn Gly Lys Val Leu Lys Gln Leu Gly Thr Val Thr Thr 
145                 150                 155                 160 


Thr Glu His Glu Asn Ala Leu Leu Arg Ser Phe Asp Lys Phe Thr Thr 
                165                 170                 175     


Tyr Phe Ser Gly Phe Tyr Glu Asn Arg Lys Asn Val Phe Ser Ala Glu 
            180                 185                 190         


Asp Ile Ser Thr Ala Ile Pro His Arg Ile Val Gln Asp Asn Phe Pro 
        195                 200                 205             


Lys Phe Lys Glu Asn Cys His Ile Phe Thr Arg Leu Ile Thr Ala Val 
    210                 215                 220                 


Pro Ser Leu Arg Glu His Phe Glu Asn Val Lys Lys Ala Ile Gly Ile 
225                 230                 235                 240 


Phe Val Ser Thr Ser Ile Glu Glu Val Phe Ser Phe Pro Phe Tyr Asn 
                245                 250                 255     


Gln Leu Leu Thr Gln Thr Gln Ile Asp Leu Tyr Asn Gln Leu Leu Gly 
            260                 265                 270         


Gly Ile Ser Arg Glu Ala Gly Thr Glu Lys Ile Lys Gly Leu Asn Glu 
        275                 280                 285             


Val Leu Asn Leu Ala Ile Gln Lys Asn Asp Glu Thr Ala His Ile Ile 
    290                 295                 300                 


Ala Ser Leu Pro His Arg Phe Ile Pro Leu Phe Lys Gln Ile Leu Ser 
305                 310                 315                 320 


Asp Arg Asn Thr Leu Ser Phe Ile Leu Glu Glu Phe Lys Ser Asp Glu 
                325                 330                 335     


Glu Val Ile Gln Ser Phe Cys Lys Tyr Lys Thr Leu Leu Arg Asn Glu 
            340                 345                 350         


Asn Val Leu Glu Thr Ala Glu Ala Leu Phe Asn Glu Leu Asn Ser Ile 
        355                 360                 365             


Asp Leu Thr His Ile Phe Ile Ser His Lys Lys Leu Glu Thr Ile Ser 
    370                 375                 380                 


Ser Ala Leu Cys Asp His Trp Asp Thr Leu Arg Asn Ala Leu Tyr Glu 
385                 390                 395                 400 


Arg Arg Ile Ser Glu Leu Thr Gly Lys Ile Thr Lys Ser Ala Lys Glu 
                405                 410                 415     


Lys Val Gln Arg Ser Leu Lys His Glu Asp Ile Asn Leu Gln Glu Ile 
            420                 425                 430         


Ile Ser Ala Ala Gly Lys Glu Leu Ser Glu Ala Phe Lys Gln Lys Thr 
        435                 440                 445             


Ser Glu Ile Leu Ser His Ala His Ala Ala Leu Asp Gln Pro Leu Pro 
    450                 455                 460                 


Thr Thr Leu Lys Lys Gln Glu Glu Lys Glu Ile Leu Lys Ser Gln Leu 
465                 470                 475                 480 


Asp Ser Leu Leu Gly Leu Tyr His Leu Leu Asp Trp Phe Ala Val Asp 
                485                 490                 495     


Glu Ser Asn Glu Val Asp Pro Glu Phe Ser Ala Arg Leu Thr Gly Ile 
            500                 505                 510         


Lys Leu Glu Met Glu Pro Ser Leu Ser Phe Tyr Asn Lys Ala Arg Asn 
        515                 520                 525             


Tyr Ala Thr Lys Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Asn Phe 
    530                 535                 540                 


Gln Met Pro Thr Leu Ala Arg Gly Trp Asp Val Asn Val Glu Lys Asn 
545                 550                 555                 560 


Arg Gly Ala Ile Leu Phe Val Lys Asn Gly Leu Tyr Tyr Leu Gly Ile 
                565                 570                 575     


Met Pro Lys Gln Lys Gly Arg Tyr Lys Ala Leu Ser Phe Glu Pro Thr 
            580                 585                 590         


Glu Lys Thr Ser Glu Gly Phe Asp Lys Met Tyr Tyr Asp Tyr Phe Pro 
        595                 600                 605             


Asp Ala Ala Lys Met Ile Pro Lys Cys Ser Thr Gln Leu Lys Ala Val 
    610                 615                 620                 


Thr Ala His Phe Gln Thr His Thr Thr Pro Ile Leu Leu Ser Asn Asn 
625                 630                 635                 640 


Phe Ile Glu Pro Leu Glu Ile Thr Lys Glu Ile Tyr Asp Leu Asn Asn 
                645                 650                 655     


Pro Glu Lys Glu Pro Lys Lys Phe Gln Thr Ala Tyr Ala Lys Lys Thr 
            660                 665                 670         


Gly Asp Gln Lys Gly Tyr Arg Glu Ala Leu Cys Lys Trp Ile Asp Phe 
        675                 680                 685             


Thr Arg Asp Phe Leu Ser Lys Tyr Thr Lys Thr Thr Ser Ile Asp Leu 
    690                 695                 700                 


Ser Ser Leu Arg Pro Ser Ser Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr 
705                 710                 715                 720 


Ala Glu Leu Asn Pro Leu Leu Tyr His Ile Ser Phe Gln Arg Ile Ala 
                725                 730                 735     


Glu Lys Glu Ile Met Asp Ala Val Glu Thr Gly Lys Leu Tyr Leu Phe 
            740                 745                 750         


Gln Ile Tyr Asn Lys Asp Phe Ala Lys Gly His His Gly Lys Pro Asn 
        755                 760                 765             


Leu His Thr Leu Tyr Trp Thr Gly Leu Phe Ser Pro Glu Asn Leu Ala 
    770                 775                 780                 


Lys Thr Ser Ile Lys Leu Asn Gly Gln Ala Glu Leu Phe Tyr Arg Pro 
785                 790                 795                 800 


Lys Ser Arg Met Lys Arg Met Ala Ala Arg Leu Gly Glu Lys Met Leu 
                805                 810                 815     


Asn Lys Lys Leu Lys Asp Gln Lys Thr Pro Ile Pro Asp Thr Leu Tyr 
            820                 825                 830         


Gln Glu Leu Tyr Asp Tyr Val Asn His Arg Leu Ser His Asp Leu Ser 
        835                 840                 845             


Asp Glu Ala Arg Ala Leu Leu Pro Asn Val Ile Thr Lys Glu Val Ser 
    850                 855                 860                 


His Glu Ile Ile Lys Asp Arg Arg Phe Thr Ser Asp Lys Phe Phe Phe 
865                 870                 875                 880 


His Val Pro Ile Thr Leu Asn Tyr Gln Ala Ala Asn Ser Pro Ser Lys 
                885                 890                 895     


Phe Asn Gln Arg Val Asn Ala Tyr Leu Lys Glu His Pro Glu Thr Pro 
            900                 905                 910         


Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Ile Tyr Ile Thr Val 
        915                 920                 925             


Ile Asp Ser Thr Gly Lys Ile Leu Glu Gln Arg Ser Leu Asn Thr Ile 
    930                 935                 940                 


Gln Gln Phe Asp Tyr Gln Lys Lys Leu Asp Asn Arg Glu Lys Glu Arg 
945                 950                 955                 960 


Val Ala Ala Arg Gln Ala Trp Ser Val Val Gly Thr Ile Lys Asp Leu 
                965                 970                 975     


Lys Gln Gly Tyr Leu Ser Gln Val Ile His Glu Ile Val Asp Leu Met 
            980                 985                 990         


Ile His Tyr Gln Ala Val Val Val  Leu Glu Asn Leu Asn  Phe Gly Phe 
        995                 1000                 1005             


Lys Ser  Lys Arg Thr Gly Ile  Ala Glu Lys Ala Val  Tyr Gln Gln 
    1010                 1015                 1020             


Phe Glu  Lys Met Leu Ile Asp  Lys Leu Asn Cys Leu  Val Leu Lys 
    1025                 1030                 1035             


Asp Tyr  Pro Ala Glu Lys Val  Gly Gly Val Leu Asn  Pro Tyr Gln 
    1040                 1045                 1050             


Leu Thr  Asp Gln Phe Thr Ser  Phe Ala Lys Met Gly  Thr Gln Ser 
    1055                 1060                 1065             


Gly Phe  Leu Phe Tyr Val Pro  Ala Pro Tyr Thr Ser  Lys Ile Asp 
    1070                 1075                 1080             


Pro Leu  Thr Gly Phe Val Asp  Pro Phe Val Trp Lys  Thr Ile Lys 
    1085                 1090                 1095             


Asn His  Glu Ser Arg Lys His  Phe Leu Glu Gly Phe  Asp Phe Leu 
    1100                 1105                 1110             


His Tyr  Asp Val Lys Thr Gly  Asp Phe Ile Leu His  Phe Lys Met 
    1115                 1120                 1125             


Asn Arg  Asn Leu Ser Phe Gln  Arg Gly Leu Pro Gly  Phe Met Pro 
    1130                 1135                 1140             


Ala Trp  Asp Ile Val Phe Glu  Lys Asn Glu Thr Gln  Phe Asp Ala 
    1145                 1150                 1155             


Lys Gly  Thr Pro Phe Ile Ala  Gly Lys Arg Ile Val  Pro Val Ile 
    1160                 1165                 1170             


Glu Asn  His Arg Phe Thr Gly  Arg Tyr Arg Asp Leu  Tyr Pro Ala 
    1175                 1180                 1185             


Asn Glu  Leu Ile Ala Leu Leu  Glu Glu Lys Gly Ile  Val Phe Arg 
    1190                 1195                 1200             


Asp Gly  Ser Asn Ile Leu Pro  Lys Leu Leu Glu Asn  Asp Asp Ser 
    1205                 1210                 1215             


His Ala  Ile Asp Thr Met Val  Ala Leu Ile Arg Ser  Val Leu Gln 
    1220                 1225                 1230             


Met Arg  Asn Ser Asn Ala Ala  Thr Gly Glu Asp Tyr  Ile Asn Ser 
    1235                 1240                 1245             


Pro Val  Arg Asp Leu Asn Gly  Val Cys Phe Asp Ser  Arg Phe Gln 
    1250                 1255                 1260             


Asn Pro  Glu Trp Pro Met Asp  Ala Asp Ala Asn Gly  Ala Tyr His 
    1265                 1270                 1275             


Ile Ala  Leu Lys Gly Gln Leu  Leu Leu Asn His Leu  Lys Glu Ser 
    1280                 1285                 1290             


Lys Asp  Leu Lys Leu Gln Asn  Gly Ile Ser Asn Gln  Asp Trp Leu 
    1295                 1300                 1305             


Ala Tyr  Ile Gln Glu Leu Arg  Asn Gly Ser Pro Lys  Lys Lys Arg 
    1310                 1315                 1320             


Lys Val  Gly Ser Pro Lys Lys  Lys Arg Lys Val 
    1325                 1330                 


<210>  1040
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ptx ssODN 18nt - positive strand (OLI16431)


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Linked by phosphorothioate (PS) bond

<400>  1040
ccctggcctc actggatact ctaagactat tggtcaagtt tgccttgtca aggctggcca       60

acccatgggt ggagtttagc cagggaccgt ttcagacaga                            100


<210>  1041
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (39)..(39)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  Linked by phosphorodithioate (PS2) bond

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  Modified to contain 2'-O-methyl

<400>  1041
uaauuucuac ucuuguagau ccuugucaag gcuauugguc                             40


<210>  1042
<211>  45
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1042
cuuuuuaauu ucuacucuug uagauccuug ucaaggcuau ugguc                       45


<210>  1043
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1043
aagaccuuuu uaauuucuac ucuuguagau ccuugucaag gcuauugguc                  50


<210>  1044
<211>  65
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1044
auguguuuuu gucaaaagac cuuuuuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguc                                                                   65


<210>  1045
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1045
aggccagcuu gccgguuuuu uagucgugcu gcuucaugug uuuuugucaa aagaccuuuu       60

uaauuucuac ucuuguagau ccuugucaag gcuauugguc                            100


<210>  1046
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (6)..(45)
<223>  RNA nucleotides

<400>  1046
cttttuaauu ucuacucuug uagauccuug ucaaggcuau ugguc                       45


<210>  1047
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (11)..(50)
<223>  RNA nucleotides

<400>  1047
aagacctttt uaauuucuac ucuuguagau ccuugucaag gcuauugguc                  50


<210>  1048
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (26)..(65)
<223>  RNA nucleotides

<400>  1048
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguc                                                                   65


<210>  1049
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(60)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (61)..(100)
<223>  RNA nucleotides

<400>  1049
aggccagctt gccggttttt tagtcgtgct gcttcatgtg tttttgtcaa aagacctttt       60

uaauuucuac ucuuguagau ccuugucaag gcuauugguc                            100


<210>  1050
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorodithioate (PS2) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1050
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguca                                                                  66


<210>  1051
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1051
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguca                                                                  66


<210>  1052
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (21)..(60)
<223>  RNA nucleotides

<400>  1052
tttttgtcaa aagacctttt uaauuucuac ucuuguagau ccuugucaag gcuauugguc       60


<210>  1053
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (31)..(70)
<223>  RNA nucleotides

<400>  1053
gcttcatgtg tttttgtcaa aagacctttt uaauuucuac ucuuguagau ccuugucaag       60

gcuauugguc                                                              70


<210>  1054
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(50)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (51)..(90)
<223>  RNA nucleotides

<400>  1054
gccggttttt tagtcgtgct gcttcatgtg tttttgtcaa aagacctttt uaauuucuac       60

ucuuguagau ccuugucaag gcuauugguc                                        90


<210>  1055
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(40)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (41)..(80)
<223>  RNA nucleotides

<400>  1055
tagtcgtgct gcttcatgtg tttttgtcaa aagacctttt uaauuucuac ucuuguagau       60

ccuugucaag gcuauugguc                                                   80


<210>  1056
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (21)..(60)
<223>  RNA nucleotides

<400>  1056
ccgaagtttt cttcggtttt uaauuucuac ucuuguagau ccuugucaag gcuauugguc       60


<210>  1057
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(65)
<223>  RNA nucleotides

<400>  1057
tttttccgaa gttttcttcg gttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguc                                                                   65


<210>  1058
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(30)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (31)..(70)
<223>  RNA nucleotides

<400>  1058
aacgcttttt ccgaagtttt cttcggtttt uaauuucuac ucuuguagau ccuugucaag       60

gcuauugguc                                                              70


<210>  1059
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(41)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (42)..(81)
<223>  RNA nucleotides

<400>  1059
gcgttgtttt caacgctttt tccgaagttt tcttcggttt tuaauuucua cucuuguaga       60

uccuugucaa ggcuauuggu c                                                 81


<210>  1060
<211>  102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(62)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (63)..(102)
<223>  RNA nucleotides

<400>  1060
ggcttctttt gaagcctttt tgcgttgttt tcaacgcttt ttccgaagtt ttcttcggtt       60

ttuaauuucu acucuuguag auccuuguca aggcuauugg uc                         102


<210>  1061
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<400>  1061
uaauuucuac ucuuguagau ccuugucaag gcuauugguc                             40


<210>  1062
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<400>  1062
uaauuucuac ucuuguagau ccuugucaag gcuauugguc                             40


<210>  1063
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<400>  1063
uaauuucuac ucuuguagau ccuugucaag gcuauugguc                             40


<210>  1064
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<400>  1064
uaauuucuac ucuuguagau ccuugucaag gcuauugguc                             40


<210>  1065
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Modified to contain 2'-O-methyl

<400>  1065
uaauuucuac ucuuguagau ccuugucaag gcuauugguc                             40


<210>  1066
<211>  40
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Modified to contain 2'-O-methyl

<400>  1066
uaauuucuac ucuuguagau ccuugucaag gcuauugguc                             40


<210>  1067
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(65)
<223>  RNA nucleotides

<400>  1067
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguc                                                                   65


<210>  1068
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1068
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguca                                                                  66


<210>  1069
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Modified to contain 2'-O-methyl

<400>  1069
uaauuucuac ucuuguagau ccuugucaag gcuauugguc a                           41


<210>  1070
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  Linked by phosphorothioate (PS) bond

<400>  1070
uaauuucuac ucuuguagau ccuugucaag gcuauugguc au                          42


<210>  1071
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1071
uaauuucuac ucuuguagau ccuugucaag gcuauugguc u                           41


<210>  1072
<211>  42
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1072
uaauuucuac ucuuguagau ccuugucaag gcuauugguc au                          42


<210>  1073
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1073
uaauuucuac ucuuguagau ccuugucaag gcuauugguc aagu                        44


<210>  1074
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<400>  1074
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

uggucu                                                                  66


<210>  1075
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(69)
<223>  RNA nucleotides

<400>  1075
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

uggucaagu                                                               69


<210>  1076
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1076
uaauuucuac ucuuguagau ccuugucaag gcuauugguc a                           41


<210>  1077
<211>  43
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1077
uaauuucuac ucuuguagau ccuugucaag gcuauugguc aag                         43


<210>  1078
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(68)
<223>  RNA nucleotides

<400>  1078
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

uggucaag                                                                68


<210>  1079
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA

<400>  1079
uaauuucuac ucuuguagau ccuugucaag gcuauugguc uuuu                        44


<210>  1080
<211>  44
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (42)..(43)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (43)..(44)
<223>  Linked by phosphorothioate (PS) bond

<400>  1080
uaauuucuac ucuuguagau ccuugucaag gcuauugguc uuuu                        44


<210>  1081
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(69)
<223>  RNA nucleotides

<400>  1081
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

uggucuuuu                                                               69


<210>  1082
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1082
aaaaaaaaaa aaaaaaaaaa aaaaauaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguca                                                                  66


<210>  1083
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1083
tttttttttt tttttttttt tttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguca                                                                  66


<210>  1084
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (21)..(61)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (60)..(61)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  Modified to contain 2'-O-methyl

<400>  1084
ccgaagtttt cttcggtttt uaauuucuac ucuuguagau ccuugucaag gcuauugguc       60

a                                                                       61


<210>  1085
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Modified to contain 2'-O-methyl

<400>  1085
uaauuucuac ucuuguagau agacagauau uugcauugag a                           41


<210>  1086
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1086
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauagaca gauauuugca       60

uugaga                                                                  66


<210>  1087
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (21)..(61)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (60)..(61)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  Modified to contain 2'-O-methyl

<400>  1087
ccgaagtttt cttcggtttt uaauuucuac ucuuguagau agacagauau uugcauugag       60

a                                                                       61


<210>  1088
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Modified to contain 2'-O-methyl

<400>  1088
uaauuucuac ucuuguagau cauugagaua guguggggaa g                           41


<210>  1089
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1089
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagaucauug agauagugug       60

gggaag                                                                  66


<210>  1090
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (21)..(61)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (60)..(61)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  Modified to contain 2'-O-methyl

<400>  1090
ccgaagtttt cttcggtttt uaauuucuac ucuuguagau cauugagaua guguggggaa       60

g                                                                       61


<210>  1091
<211>  41
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (41)..(41)
<223>  Modified to contain 2'-O-methyl

<400>  1091
uaauuucuac ucuuguagau cuucucccau cauagaggau a                           41


<210>  1092
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1092
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagaucuucu cccaucauag       60

aggaua                                                                  66


<210>  1093
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (21)..(61)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (60)..(61)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  Modified to contain 2'-O-methyl

<400>  1093
ccgaagtttt cttcggtttt uaauuucuac ucuuguagau cuucucccau cauagaggau       60

a                                                                       61


<210>  1094
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  MISC_FEATURE
<222>  (1310)..(1331)
<223>  NLS

<220>
<221>  MISC_FEATURE
<222>  (1358)..(1363)
<223>  His tag

<400>  1094

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Arg Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Leu Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn Gly 
    1295                 1300                 1305             


Arg Ser  Ser Asp Asp Glu Ala  Thr Ala Asp Ser Gln  His Ala Ala 
    1310                 1315                 1320             


Pro Pro  Lys Lys Lys Arg Lys  Val Gly Gly Ser Gly  Gly Ser Gly 
    1325                 1330                 1335             


Gly Ser  Gly Gly Ser Gly Gly  Ser Gly Gly Ser Gly  Gly Ser Gly 
    1340                 1345                 1350             


Gly Ser  Leu Glu His His His  His His His 
    1355                 1360             


<210>  1095
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  MISC_FEATURE
<222>  (1310)..(1331)
<223>  NLS

<220>
<221>  MISC_FEATURE
<222>  (1358)..(1363)
<223>  His tag

<400>  1095

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn Gly 
    1295                 1300                 1305             


Arg Ser  Ser Asp Asp Glu Ala  Thr Ala Asp Ser Gln  His Ala Ala 
    1310                 1315                 1320             


Pro Pro  Lys Lys Lys Arg Lys  Val Gly Gly Ser Gly  Gly Ser Gly 
    1325                 1330                 1335             


Gly Ser  Gly Gly Ser Gly Gly  Ser Gly Gly Ser Gly  Gly Ser Gly 
    1340                 1345                 1350             


Gly Ser  Leu Glu His His His  His His His 
    1355                 1360             


<210>  1096
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  MISC_FEATURE
<222>  (1310)..(1331)
<223>  NLS

<220>
<221>  MISC_FEATURE
<222>  (1358)..(1363)
<223>  His tag

<400>  1096

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Arg Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala Ala 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Leu Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn Gly 
    1295                 1300                 1305             


Arg Ser  Ser Asp Asp Glu Ala  Thr Ala Asp Ser Gln  His Ala Ala 
    1310                 1315                 1320             


Pro Pro  Lys Lys Lys Arg Lys  Val Gly Gly Ser Gly  Gly Ser Gly 
    1325                 1330                 1335             


Gly Ser  Gly Gly Ser Gly Gly  Ser Gly Gly Ser Gly  Gly Ser Gly 
    1340                 1345                 1350             


Gly Ser  Leu Glu His His His  His His His 
    1355                 1360             


<210>  1097
<211>  1331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  MISC_FEATURE
<222>  (1310)..(1331)
<223>  NLS

<400>  1097

Met Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Arg Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala Ala 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Leu Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn Gly 
    1295                 1300                 1305             


Arg Ser  Ser Asp Asp Glu Ala  Thr Ala Asp Ser Gln  His Ala Ala 
    1310                 1315                 1320             


Pro Pro  Lys Lys Lys Arg Lys  Val 
    1325                 1330     


<210>  1098
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(67)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (66)..(67)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1098
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

uggucau                                                                 67


<210>  1099
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(68)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (66)..(67)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (67)..(67)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (67)..(68)
<223>  Linked by phosphorothioate (PS) bond

<400>  1099
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

uggucaau                                                                68


<210>  1100
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(66)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<400>  1100
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

uggucu                                                                  66


<210>  1101
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(65)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (64)..(65)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (65)..(65)
<223>  Modified to contain 2'-O-methyl

<400>  1101
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguc                                                                   65


<210>  1102
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  DNA nucleotides

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (26)..(67)
<223>  RNA nucleotides

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(67)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (67)..(67)
<223>  Modified to contain 2'-O-methyl

<400>  1102
atgtgttttt gtcaaaagac cttttuaauu ucuacucuug uagauccuug ucaaggcuau       60

uggucaa                                                                 67


<210>  1103
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1103
auguguuuuu gucaaaagac cuuuuuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguca                                                                  66


<210>  1104
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1104
aaaaaaaaaa aaaaaaaaaa aaaaauaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguca                                                                  66


<210>  1105
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (65)..(66)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (66)..(66)
<223>  Modified to contain 2'-O-methyl

<400>  1105
uuuuuuuuuu uuuuuuuuuu uuuuuuaauu ucuacucuug uagauccuug ucaaggcuau       60

ugguca                                                                  66


<210>  1106
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1106
uaauuucuac ucuuguagau ccuugucagg cuauuggtca ag                          42


<210>  1107
<211>  1331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  MISC_FEATURE
<222>  (1310)..(1331)
<223>  NLS

<400>  1107

Ser Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Arg Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Leu Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn Gly 
    1295                 1300                 1305             


Arg Ser  Ser Asp Asp Glu Ala  Thr Ala Asp Ser Gln  His Ala Ala 
    1310                 1315                 1320             


Pro Pro  Lys Lys Lys Arg Lys  Val 
    1325                 1330     


<210>  1108
<211>  1363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  MISC_FEATURE
<222>  (1310)..(1331)
<223>  NLS

<220>
<221>  MISC_FEATURE
<222>  (1358)..(1363)
<223>  His tag

<400>  1108

Ser Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser Lys Thr 
1               5                   10                  15      


Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His Ile Gln 
            20                  25                  30          


Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His Tyr Lys 
        35                  40                  45              


Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala Asp Gln 
    50                  55                  60                  


Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala Ala Ile 
65                  70                  75                  80  


Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala Leu Ile 
                85                  90                  95      


Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe Ile Gly 
            100                 105                 110         


Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala Glu Ile 
        115                 120                 125             


Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val Leu Lys 
    130                 135                 140                 


Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu Leu Arg 
145                 150                 155                 160 


Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu Asn Arg 
                165                 170                 175     


Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro His Arg 
            180                 185                 190         


Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His Ile Phe 
        195                 200                 205             


Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe Glu Asn 
    210                 215                 220                 


Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu Glu Val 
225                 230                 235                 240 


Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln Ile Asp 
                245                 250                 255     


Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly Thr Glu 
            260                 265                 270         


Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln Lys Asn 
        275                 280                 285             


Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe Ile Pro 
    290                 295                 300                 


Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe Ile Leu 
305                 310                 315                 320 


Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys Lys Tyr 
                325                 330                 335     


Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu Ala Leu 
            340                 345                 350         


Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile Ser His 
        355                 360                 365             


Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp Asp Thr 
    370                 375                 380                 


Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr Gly Lys 
385                 390                 395                 400 


Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys His Glu 
                405                 410                 415     


Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu Leu Ser 
            420                 425                 430         


Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala His Ala 
        435                 440                 445             


Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu Glu Lys 
    450                 455                 460                 


Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr His Leu 
465                 470                 475                 480 


Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro Glu Phe 
                485                 490                 495     


Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser Leu Ser 
            500                 505                 510         


Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr Ser Val 
        515                 520                 525             


Glu Lys Phe Lys Leu Asn Phe Gln Arg Pro Thr Leu Ala Ser Gly Trp 
    530                 535                 540                 


Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val Lys Asn 
545                 550                 555                 560 


Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg Tyr Lys 
                565                 570                 575     


Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe Asp Lys 
            580                 585                 590         


Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro Lys Cys 
        595                 600                 605             


Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His Thr Thr 
    610                 615                 620                 


Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile Thr Lys 
625                 630                 635                 640 


Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys Phe Gln 
                645                 650                 655     


Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg Glu Ala 
            660                 665                 670         


Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys Tyr Thr 
        675                 680                 685             


Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu Tyr His 
705                 710                 715                 720 


Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala Val Glu 
                725                 730                 735     


Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe Ala Lys 
            740                 745                 750         


Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr Gly Leu 
        755                 760                 765             


Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn Gly Gln 
    770                 775                 780                 


Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met Ala His 
785                 790                 795                 800 


Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln Lys Thr 
                805                 810                 815     


Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val Asn His 
            820                 825                 830         


Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu Pro Asn 
        835                 840                 845             


Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg Arg Phe 
    850                 855                 860                 


Thr Ser Asp Lys Phe Leu Phe His Val Pro Ile Thr Leu Asn Tyr Gln 
865                 870                 875                 880 


Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala Tyr Leu 
                885                 890                 895     


Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly Glu Arg 
            900                 905                 910         


Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile Leu Glu 
        915                 920                 925             


Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys Lys Leu 
    930                 935                 940                 


Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp Ser Val 
945                 950                 955                 960 


Val Gly Thr Ile Lys Asp Leu Lys Gln Gly Tyr Leu Ser Gln Val Ile 
                965                 970                 975     


His Glu Ile Val Asp Leu Met Ile His Tyr Gln Ala Val Val Val Leu 
            980                 985                 990         


Glu Asn Leu Asn Phe Gly Phe Lys  Ser Lys Arg Thr Gly  Ile Ala Glu 
        995                 1000                 1005             


Lys Ala  Val Tyr Gln Gln Phe  Glu Lys Met Leu Ile  Asp Lys Leu 
    1010                 1015                 1020             


Asn Cys  Leu Val Leu Lys Asp  Tyr Pro Ala Glu Lys  Val Gly Gly 
    1025                 1030                 1035             


Val Leu  Asn Pro Tyr Gln Leu  Thr Asp Gln Phe Thr  Ser Phe Ala 
    1040                 1045                 1050             


Lys Met  Gly Thr Gln Ser Gly  Phe Leu Phe Tyr Val  Pro Ala Pro 
    1055                 1060                 1065             


Tyr Thr  Ser Lys Ile Asp Pro  Leu Thr Gly Phe Val  Asp Pro Phe 
    1070                 1075                 1080             


Val Trp  Lys Thr Ile Lys Asn  His Glu Ser Arg Lys  His Phe Leu 
    1085                 1090                 1095             


Glu Gly  Phe Asp Phe Leu His  Tyr Asp Val Lys Thr  Gly Asp Phe 
    1100                 1105                 1110             


Ile Leu  His Phe Lys Met Asn  Arg Asn Leu Ser Phe  Gln Arg Gly 
    1115                 1120                 1125             


Leu Pro  Gly Phe Met Pro Ala  Trp Asp Ile Val Phe  Glu Lys Asn 
    1130                 1135                 1140             


Glu Thr  Gln Phe Asp Ala Lys  Gly Thr Pro Phe Ile  Ala Gly Lys 
    1145                 1150                 1155             


Arg Ile  Val Pro Val Ile Glu  Asn His Arg Phe Thr  Gly Arg Tyr 
    1160                 1165                 1170             


Arg Asp  Leu Tyr Pro Ala Asn  Glu Leu Ile Ala Leu  Leu Glu Glu 
    1175                 1180                 1185             


Lys Gly  Ile Val Phe Arg Asp  Gly Ser Asn Ile Leu  Pro Lys Leu 
    1190                 1195                 1200             


Leu Glu  Asn Asp Asp Ser His  Ala Ile Asp Thr Met  Val Ala Leu 
    1205                 1210                 1215             


Ile Arg  Ser Val Leu Gln Met  Arg Asn Ser Asn Ala  Ala Thr Gly 
    1220                 1225                 1230             


Glu Asp  Tyr Ile Asn Ser Pro  Val Arg Asp Leu Asn  Gly Val Cys 
    1235                 1240                 1245             


Phe Asp  Ser Arg Phe Gln Asn  Pro Glu Trp Pro Met  Asp Ala Asp 
    1250                 1255                 1260             


Ala Asn  Gly Ala Tyr His Ile  Ala Leu Lys Gly Gln  Leu Leu Leu 
    1265                 1270                 1275             


Asn His  Leu Lys Glu Ser Lys  Asp Leu Lys Leu Gln  Asn Gly Ile 
    1280                 1285                 1290             


Ser Asn  Gln Asp Trp Leu Ala  Tyr Ile Gln Glu Leu  Arg Asn Gly 
    1295                 1300                 1305             


Arg Ser  Ser Asp Asp Glu Ala  Thr Ala Asp Ser Gln  His Ala Ala 
    1310                 1315                 1320             


Pro Pro  Lys Lys Lys Arg Lys  Val Gly Gly Ser Gly  Gly Ser Gly 
    1325                 1330                 1335             


Gly Ser  Gly Gly Ser Gly Gly  Ser Gly Gly Ser Gly  Gly Ser Gly 
    1340                 1345                 1350             


Gly Ser  Leu Glu His His His  His His His 
    1355                 1360             


<210>  1109
<211>  1361
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  MISC_FEATURE
<222>  (1342)..(1348)
<223>  NLS

<220>
<221>  MISC_FEATURE
<222>  (1356)..(1361)
<223>  His tag

<400>  1109

Met Gly Arg Asp Pro Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu 
1               5                   10                  15      


Asp Ser Thr Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val 
            20                  25                  30          


Pro Ala Ala Thr Gln Phe Glu Gly Phe Thr Asn Leu Tyr Gln Val Ser 
        35                  40                  45              


Lys Thr Leu Arg Phe Glu Leu Ile Pro Gln Gly Lys Thr Leu Lys His 
    50                  55                  60                  


Ile Gln Glu Gln Gly Phe Ile Glu Glu Asp Lys Ala Arg Asn Asp His 
65                  70                  75                  80  


Tyr Lys Glu Leu Lys Pro Ile Ile Asp Arg Ile Tyr Lys Thr Tyr Ala 
                85                  90                  95      


Asp Gln Cys Leu Gln Leu Val Gln Leu Asp Trp Glu Asn Leu Ser Ala 
            100                 105                 110         


Ala Ile Asp Ser Tyr Arg Lys Glu Lys Thr Glu Glu Thr Arg Asn Ala 
        115                 120                 125             


Leu Ile Glu Glu Gln Ala Thr Tyr Arg Asn Ala Ile His Asp Tyr Phe 
    130                 135                 140                 


Ile Gly Arg Thr Asp Asn Leu Thr Asp Ala Ile Asn Lys Arg His Ala 
145                 150                 155                 160 


Glu Ile Tyr Lys Gly Leu Phe Lys Ala Glu Leu Phe Asn Gly Lys Val 
                165                 170                 175     


Leu Lys Gln Leu Gly Thr Val Thr Thr Thr Glu His Glu Asn Ala Leu 
            180                 185                 190         


Leu Arg Ser Phe Asp Lys Phe Thr Thr Tyr Phe Ser Gly Phe Tyr Glu 
        195                 200                 205             


Asn Arg Lys Asn Val Phe Ser Ala Glu Asp Ile Ser Thr Ala Ile Pro 
    210                 215                 220                 


His Arg Ile Val Gln Asp Asn Phe Pro Lys Phe Lys Glu Asn Cys His 
225                 230                 235                 240 


Ile Phe Thr Arg Leu Ile Thr Ala Val Pro Ser Leu Arg Glu His Phe 
                245                 250                 255     


Glu Asn Val Lys Lys Ala Ile Gly Ile Phe Val Ser Thr Ser Ile Glu 
            260                 265                 270         


Glu Val Phe Ser Phe Pro Phe Tyr Asn Gln Leu Leu Thr Gln Thr Gln 
        275                 280                 285             


Ile Asp Leu Tyr Asn Gln Leu Leu Gly Gly Ile Ser Arg Glu Ala Gly 
    290                 295                 300                 


Thr Glu Lys Ile Lys Gly Leu Asn Glu Val Leu Asn Leu Ala Ile Gln 
305                 310                 315                 320 


Lys Asn Asp Glu Thr Ala His Ile Ile Ala Ser Leu Pro His Arg Phe 
                325                 330                 335     


Ile Pro Leu Phe Lys Gln Ile Leu Ser Asp Arg Asn Thr Leu Ser Phe 
            340                 345                 350         


Ile Leu Glu Glu Phe Lys Ser Asp Glu Glu Val Ile Gln Ser Phe Cys 
        355                 360                 365             


Lys Tyr Lys Thr Leu Leu Arg Asn Glu Asn Val Leu Glu Thr Ala Glu 
    370                 375                 380                 


Ala Leu Phe Asn Glu Leu Asn Ser Ile Asp Leu Thr His Ile Phe Ile 
385                 390                 395                 400 


Ser His Lys Lys Leu Glu Thr Ile Ser Ser Ala Leu Cys Asp His Trp 
                405                 410                 415     


Asp Thr Leu Arg Asn Ala Leu Tyr Glu Arg Arg Ile Ser Glu Leu Thr 
            420                 425                 430         


Gly Lys Ile Thr Lys Ser Ala Lys Glu Lys Val Gln Arg Ser Leu Lys 
        435                 440                 445             


His Glu Asp Ile Asn Leu Gln Glu Ile Ile Ser Ala Ala Gly Lys Glu 
    450                 455                 460                 


Leu Ser Glu Ala Phe Lys Gln Lys Thr Ser Glu Ile Leu Ser His Ala 
465                 470                 475                 480 


His Ala Ala Leu Asp Gln Pro Leu Pro Thr Thr Leu Lys Lys Gln Glu 
                485                 490                 495     


Glu Lys Glu Ile Leu Lys Ser Gln Leu Asp Ser Leu Leu Gly Leu Tyr 
            500                 505                 510         


His Leu Leu Asp Trp Phe Ala Val Asp Glu Ser Asn Glu Val Asp Pro 
        515                 520                 525             


Glu Phe Ser Ala Arg Leu Thr Gly Ile Lys Leu Glu Met Glu Pro Ser 
    530                 535                 540                 


Leu Ser Phe Tyr Asn Lys Ala Arg Asn Tyr Ala Thr Lys Lys Pro Tyr 
545                 550                 555                 560 


Ser Val Glu Lys Phe Lys Leu Asn Phe Gln Met Pro Thr Leu Ala Ser 
                565                 570                 575     


Gly Trp Asp Val Asn Lys Glu Lys Asn Asn Gly Ala Ile Leu Phe Val 
            580                 585                 590         


Lys Asn Gly Leu Tyr Tyr Leu Gly Ile Met Pro Lys Gln Lys Gly Arg 
        595                 600                 605             


Tyr Lys Ala Leu Ser Phe Glu Pro Thr Glu Lys Thr Ser Glu Gly Phe 
    610                 615                 620                 


Asp Lys Met Tyr Tyr Asp Tyr Phe Pro Asp Ala Ala Lys Met Ile Pro 
625                 630                 635                 640 


Lys Cys Ser Thr Gln Leu Lys Ala Val Thr Ala His Phe Gln Thr His 
                645                 650                 655     


Thr Thr Pro Ile Leu Leu Ser Asn Asn Phe Ile Glu Pro Leu Glu Ile 
            660                 665                 670         


Thr Lys Glu Ile Tyr Asp Leu Asn Asn Pro Glu Lys Glu Pro Lys Lys 
        675                 680                 685             


Phe Gln Thr Ala Tyr Ala Lys Lys Thr Gly Asp Gln Lys Gly Tyr Arg 
    690                 695                 700                 


Glu Ala Leu Cys Lys Trp Ile Asp Phe Thr Arg Asp Phe Leu Ser Lys 
705                 710                 715                 720 


Tyr Thr Lys Thr Thr Ser Ile Asp Leu Ser Ser Leu Arg Pro Ser Ser 
                725                 730                 735     


Gln Tyr Lys Asp Leu Gly Glu Tyr Tyr Ala Glu Leu Asn Pro Leu Leu 
            740                 745                 750         


Tyr His Ile Ser Phe Gln Arg Ile Ala Glu Lys Glu Ile Met Asp Ala 
        755                 760                 765             


Val Glu Thr Gly Lys Leu Tyr Leu Phe Gln Ile Tyr Asn Lys Asp Phe 
    770                 775                 780                 


Ala Lys Gly His His Gly Lys Pro Asn Leu His Thr Leu Tyr Trp Thr 
785                 790                 795                 800 


Gly Leu Phe Ser Pro Glu Asn Leu Ala Lys Thr Ser Ile Lys Leu Asn 
                805                 810                 815     


Gly Gln Ala Glu Leu Phe Tyr Arg Pro Lys Ser Arg Met Lys Arg Met 
            820                 825                 830         


Ala His Arg Leu Gly Glu Lys Met Leu Asn Lys Lys Leu Lys Asp Gln 
        835                 840                 845             


Lys Thr Pro Ile Pro Asp Thr Leu Tyr Gln Glu Leu Tyr Asp Tyr Val 
    850                 855                 860                 


Asn His Arg Leu Ser His Asp Leu Ser Asp Glu Ala Arg Ala Leu Leu 
865                 870                 875                 880 


Pro Asn Val Ile Thr Lys Glu Val Ser His Glu Ile Ile Lys Asp Arg 
                885                 890                 895     


Arg Phe Thr Ser Asp Lys Phe Phe Phe His Val Pro Ile Thr Leu Asn 
            900                 905                 910         


Tyr Gln Ala Ala Asn Ser Pro Ser Lys Phe Asn Gln Arg Val Asn Ala 
        915                 920                 925             


Tyr Leu Lys Glu His Pro Glu Thr Pro Ile Ile Gly Ile Asp Arg Gly 
    930                 935                 940                 


Glu Arg Asn Leu Ile Tyr Ile Thr Val Ile Asp Ser Thr Gly Lys Ile 
945                 950                 955                 960 


Leu Glu Gln Arg Ser Leu Asn Thr Ile Gln Gln Phe Asp Tyr Gln Lys 
                965                 970                 975     


Lys Leu Asp Asn Arg Glu Lys Glu Arg Val Ala Ala Arg Gln Ala Trp 
            980                 985                 990         


Ser Val Val Gly Thr Ile Lys Asp  Leu Lys Gln Gly Tyr  Leu Ser Gln 
        995                 1000                 1005             


Val Ile  His Glu Ile Val Asp  Leu Met Ile His Tyr  Gln Ala Val 
    1010                 1015                 1020             


Val Val  Leu Glu Asn Leu Asn  Phe Gly Phe Lys Ser  Lys Arg Thr 
    1025                 1030                 1035             


Gly Ile  Ala Glu Lys Ala Val  Tyr Gln Gln Phe Glu  Lys Met Leu 
    1040                 1045                 1050             


Ile Asp  Lys Leu Asn Cys Leu  Val Leu Lys Asp Tyr  Pro Ala Glu 
    1055                 1060                 1065             


Lys Val  Gly Gly Val Leu Asn  Pro Tyr Gln Leu Thr  Asp Gln Phe 
    1070                 1075                 1080             


Thr Ser  Phe Ala Lys Met Gly  Thr Gln Ser Gly Phe  Leu Phe Tyr 
    1085                 1090                 1095             


Val Pro  Ala Pro Tyr Thr Ser  Lys Ile Asp Pro Leu  Thr Gly Phe 
    1100                 1105                 1110             


Val Asp  Pro Phe Val Trp Lys  Thr Ile Lys Asn His  Glu Ser Arg 
    1115                 1120                 1125             


Lys His  Phe Leu Glu Gly Phe  Asp Phe Leu His Tyr  Asp Val Lys 
    1130                 1135                 1140             


Thr Gly  Asp Phe Ile Leu His  Phe Lys Met Asn Arg  Asn Leu Ser 
    1145                 1150                 1155             


Phe Gln  Arg Gly Leu Pro Gly  Phe Met Pro Ala Trp  Asp Ile Val 
    1160                 1165                 1170             


Phe Glu  Lys Asn Glu Thr Gln  Phe Asp Ala Lys Gly  Thr Pro Phe 
    1175                 1180                 1185             


Ile Ala  Gly Lys Arg Ile Val  Pro Val Ile Glu Asn  His Arg Phe 
    1190                 1195                 1200             


Thr Gly  Arg Tyr Arg Asp Leu  Tyr Pro Ala Asn Glu  Leu Ile Ala 
    1205                 1210                 1215             


Leu Leu  Glu Glu Lys Gly Ile  Val Phe Arg Asp Gly  Ser Asn Ile 
    1220                 1225                 1230             


Leu Pro  Lys Leu Leu Glu Asn  Asp Asp Ser His Ala  Ile Asp Thr 
    1235                 1240                 1245             


Met Val  Ala Leu Ile Arg Ser  Val Leu Gln Met Arg  Asn Ser Asn 
    1250                 1255                 1260             


Ala Ala  Thr Gly Glu Asp Tyr  Ile Asn Ser Pro Val  Arg Asp Leu 
    1265                 1270                 1275             


Asn Gly  Val Cys Phe Asp Ser  Arg Phe Gln Asn Pro  Glu Trp Pro 
    1280                 1285                 1290             


Met Asp  Ala Asp Ala Asn Gly  Ala Tyr His Ile Ala  Leu Lys Gly 
    1295                 1300                 1305             


Gln Leu  Leu Leu Asn His Leu  Lys Glu Ser Lys Asp  Leu Lys Leu 
    1310                 1315                 1320             


Gln Asn  Gly Ile Ser Asn Gln  Asp Trp Leu Ala Tyr  Ile Gln Glu 
    1325                 1330                 1335             


Leu Arg  Asn Pro Lys Lys Lys  Arg Lys Val Lys Leu  Ala Ala Ala 
    1340                 1345                 1350             


Leu Glu  His His His His His  His 
    1355                 1360     


<210>  1110
<211>  4002
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  His-AsCpf1-sNLS-sNLS H800A


<220>
<221>  misc_feature
<222>  (7)..(24)
<223>  His tag

<220>
<221>  misc_feature
<222>  (3955)..(3975)
<223>  sNLS

<220>
<221>  misc_feature
<222>  (3982)..(4002)
<223>  sNLS

<400>  1110
atgggccatc atcatcacca tcacggtagc acccagtttg aaggctttac caatctgtat       60

caggttagca aaaccctgcg ttttgaactg attccgcagg gtaaaaccct gaaacatatt      120

caagaacagg gcttcatcga agaggataaa gcacgtaacg atcactacaa agaactgaaa      180

ccgattatcg accgcatcta taaaacgtac gcagatcagt gtctgcagct ggttcagctg      240

gattgggaaa atctgagcgc agcaattgat agttatcgca aagagaaaac cgaagaaacc      300

cgtaatgcac tgattgaaga acaggcaacc tatcgtaatg ccatccatga ttatttcatt      360

ggtcgtaccg ataatctgac cgatgcaatt aacaaacgtc acgccgaaat ctataaaggc      420

ctgtttaaag ccgaactgtt taatggcaaa gttctgaaac agctgggcac cgttaccacc      480

accgaacatg aaaatgcact gctgcgtagc tttgataaat tcaccaccta tttcagcggc      540

ttttatgaga atcgcaaaaa cgtgtttagc gcagaagata ttagcaccgc aattccgcat      600

cgtattgtgc aggataattt cccgaaattc aaagagaact gccacatttt tacccgtctg      660

attaccgcag ttccgagcct gcgtgaacat tttgaaaacg ttaaaaaagc catcggcatc      720

tttgttagca ccagcattga agaagttttt agcttcccgt tttacaatca gctgctgacc      780

cagacacaga ttgatctgta taaccagctg ttaggtggta ttagccgtga agcaggcacc      840

gaaaaaatca aaggtctgaa tgaagtgctg aatctggcca ttcagaaaaa tgatgaaacc      900

gcacatatta ttgcaagcct gccgcatcgt tttattccgc tgtttaagca gattctgagc      960

gatcgtaata ccctgagctt tattctggaa gaattcaaat ccgatgaaga ggtgattcag     1020

agcttctgca aatacaaaac gctgctgcgc aatgaaaatg ttctggaaac tgccgaagca     1080

ctgtttaacg aactgaatag cattgatctg acccacatct ttatcagcca caaaaaactg     1140

gaaaccattt caagcgcact gtgtgatcat tgggataccc tgcgtaatgc cctgtatgaa     1200

cgtcgtatta gcgaactgac cggtaaaatt accaaaagcg cgaaagaaaa agttcagcgc     1260

agtctgaaac atgaggatat taatctgcaa gagattatta gcgcagccgg taaagaactg     1320

tcagaagcat ttaaacagaa aaccagcgaa attctgagcc atgcacatgc agcactggat     1380

cagccgctgc cgaccacact gaaaaaacaa gaagaaaaag agatcctgaa aagccagctg     1440

gatagcctgc tgggtctgta tcatctgctg gactggtttg cagttgatga aagcaatgaa     1500

gttgatccgg aatttagcgc acgtctgacc ggcattaagc tggaaatgga accgagcctg     1560

agtttttaca ataaagcccg taattatgcc accaaaaaac cgtatagcgt ggaaaaattc     1620

aagctgaact tccagatgcc gacactggca agcggttggg atgttaataa agaaaagaac     1680

aatggtgcca tcctgttcgt gaaaaatggc ctgtattatc tgggtatcat gccgaaacag     1740

aaaggtcgtt ataaagcact gagctttgaa ccgacagaaa aaaccagtga aggcttcgat     1800

aaaatgtact atgattactt tccggacgca gccaaaatga ttccgaaatg cagcacccag     1860

ctgaaagcag ttaccgcaca ttttcagacc cataccacac cgattctgct gagcaataac     1920

tttattgaac cgctggaaat caccaaagag atctacgatc tgaataaccc ggaaaaagag     1980

ccgaaaaagt ttcagaccgc ctatgcaaaa aaaaccggtg atcagaaagg ttatcgtgaa     2040

gcgctgtgta aatggattga tttcacccgt gattttctga gcaaatacac caaaaccacc     2100

agtatcgatc tgagcagcct gcgtccgagc agccagtata aagatctggg cgaatattat     2160

gcagaactga atccgctgct gtatcatatt agctttcagc gtattgccga gaaagaaata     2220

atggatgcag ttgaaaccgg taagctgtac ctgttccaga tttataacaa agactttgcc     2280

aaaggccatc atggtaaacc gaatctgcat accctgtatt ggaccggtct gtttagccct     2340

gaaaatctgg caaaaaccag cattaaactg aatggtcagg cggaactgtt ttatcgtccg     2400

aaaagccgta tgaaacgtat ggcagcacgt ctgggtgaaa aaatgctgaa taaaaagctg     2460

aaggatcaga aaaccccgat tccggataca ctgtatcaag agctgtatga ttatgtgaat     2520

catcgtctga gtcatgatct gagtgatgaa gcacgtgccc tgctgccgaa tgttattacc     2580

aaagaagtta gccacgagat cattaaagat cgtcgtttta ccagcgacaa gttttttttc     2640

catgttccga ttacgctgaa ttatcaggca gcaaatagcc cgagcaaatt taaccagcgt     2700

gtgaatgcat atctgaaaga acatccggaa acaccgatta ttggtattga tcgtggtgaa     2760

cgcaacctga tttatatcac cgttattgat agcaccggca agatcctgga acagcgtagc     2820

ctgaatacca ttcagcagtt tgattatcag aaaaagctgg acaaccgtga aaaagaacgt     2880

gttgcagccc gtcaggcatg gtcagttgtt ggtacaatta aagacctgaa acagggttat     2940

ctgagccagg ttattcatga aattgtggat ctgatgattc attaccaggc agttgttgtg     3000

ctggaaaacc tgaattttgg ctttaaaagc aaacgtaccg gcattgcgga aaaagcagtt     3060

tatcagcagt tcgaaaagat gctgatcgat aaactgaact gtctggtgct gaaagattat     3120

ccggctgaaa aagttggcgg tgttctgaat ccgtatcagc tgaccgatca gtttaccagc     3180

tttgcaaaaa tgggcaccca gagcggattt ctgttttatg ttccggcacc gtatacgagc     3240

aaaattgatc cgctgaccgg ttttgttgat ccgtttgttt ggaaaaccat caaaaaccat     3300

gaaagccgca aacattttct ggaaggtttt gatttcctgc attacgacgt taagaccggt     3360

gattttattc tgcactttaa gatgaatcgg aacctgtcat ttcagcgtgg tctgcctggt     3420

tttatgcctg catgggatat tgtgtttgag aaaaacgaaa cacagttcga tgcaaaaggc     3480

accccgttta ttgcaggtaa acgtattgtt ccggtgattg aaaatcatcg tttcaccggt     3540

cgttatcgcg atctgtatcc ggcaaatgaa ctgatcgcac tgctggaaga gaaaggtatt     3600

gtttttcgtg atggctcaaa cattctgccg aaactgttag agaatgatga tagccatgcc     3660

attgatacca tggttgccct gattcgtagc gttctgcaga tgcgtaatag caatgcagca     3720

accggtgaag attacattaa tagtccggtt cgtgatctga atggtgtttg ttttgatagc     3780

cgttttcaga atccggaatg gccgatggat gcggatgcaa atggtgcata tcatattgca     3840

ctgaaaggac agctgctgct gaaccacctg aaagaaagta aagatttgaa gctgcagaat     3900

ggcattagca atcaggattg gctggcatat atccaagaac tgcgtaacgg ttctcctaag     3960

aaaaagcgca aagttggcag tccgaagaag aaacgtaagg tt                        4002


<210>  1111
<211>  4089
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  misc_feature
<222>  (3928)..(3993)
<223>  NLS

<220>
<221>  misc_feature
<222>  (4072)..(4089)
<223>  His tag

<400>  1111
atgacccagt ttgaaggttt caccaatctg tatcaggtta gcaaaaccct gcgttttgaa       60

ctgattccgc agggtaaaac cctgaaacat attcaagaac agggcttcat cgaagaggat      120

aaagcacgta acgatcacta caaagaactg aaaccgatta tcgaccgcat ctataaaacc      180

tatgcagatc agtgtctgca gctggttcag ctggattggg aaaatctgag cgcagcaatt      240

gatagttatc gcaaagaaaa aaccgaagaa acccgtaatg cactgattga agaacaggca      300

acctatcgta atgccatcca tgattatttc attggtcgta ccgataatct gaccgatgca      360

attaacaaac gtcacgccga aatctataaa ggcctgttta aagccgaact gtttaatggc      420

aaagttctga aacagctggg caccgttacc accaccgaac atgaaaatgc actgctgcgt      480

agctttgata aattcaccac ctatttcagc ggcttttatg agaatcgcaa aaacgtgttt      540

agcgcagaag atattagcac cgcaattccg catcgtattg tgcaggataa tttcccgaaa      600

ttcaaagaga actgccacat ttttacccgt ctgattaccg cagttccgag cctgcgtgaa      660

cattttgaaa acgttaaaaa agccatcggc atctttgtta gcaccagcat tgaagaagtt      720

tttagcttcc cgttttacaa tcagctgctg acccagaccc agattgatct gtataaccaa      780

ctgctgggtg gtattagccg tgaagcaggc accgaaaaaa tcaaaggtct gaatgaagtg      840

ctgaatctgg ccattcagaa aaatgatgaa accgcacata ttattgcaag cctgccgcat      900

cgttttattc cgctgttcaa acaaattctg agcgatcgta ataccctgag ctttattctg      960

gaagaattca aatccgatga agaggtgatt cagagctttt gcaaatacaa aacgctgctg     1020

cgcaatgaaa atgttctgga aactgccgaa gcactgttta acgaactgaa tagcattgat     1080

ctgacccaca tctttatcag ccacaaaaaa ctggaaacca tttcaagcgc actgtgtgat     1140

cattgggata ccctgcgtaa tgccctgtat gaacgtcgta ttagcgaact gaccggtaaa     1200

attaccaaaa gcgcgaaaga aaaagttcag cgcagtctga aacatgagga tattaatctg     1260

caagagatta ttagcgcagc cggtaaagaa ctgtcagaag catttaaaca gaaaaccagc     1320

gaaattctgt cacatgcaca tgcagcactg gatcagccgc tgccgaccac cctgaaaaaa     1380

caagaagaaa aagaaatcct gaaaagccag ctggatagcc tgctgggtct gtatcatctg     1440

ctggactggt ttgcagttga tgaaagcaat gaagttgatc cggaatttag cgcacgtctg     1500

accggcatta aactggaaat ggaaccgagc ctgagctttt ataacaaagc ccgtaattat     1560

gccaccaaaa aaccgtatag cgtcgaaaaa ttcaaactga actttcagcg tccgaccctg     1620

gcaagcggtt gggatgttaa taaagaaaaa aacaacggtg ccatcctgtt cgtgaaaaat     1680

ggcctgtatt atctgggtat tatgccgaaa cagaaaggtc gttataaagc gctgagcttt     1740

gaaccgacgg aaaaaaccag tgaaggtttt gataaaatgt actacgacta ttttccggat     1800

gcagccaaaa tgattccgaa atgtagcacc cagctgaaag cagttaccgc acattttcag     1860

acccatacca ccccgattct gctgagcaat aactttattg aaccgctgga aatcaccaaa     1920

gagatctacg atctgaataa cccggaaaaa gagccgaaaa aattccagac cgcatatgca     1980

aaaaaaaccg gtgatcagaa aggttatcgt gaagcgctgt gtaaatggat tgatttcacc     2040

cgtgattttc tgagcaaata caccaaaacc accagtatcg atctgagcag cctgcgtccg     2100

agcagccagt ataaagatct gggcgaatat tatgcagaac tgaatccgct gctgtatcat     2160

attagctttc agcgtattgc cgagaaagaa atcatggacg cagttgaaac cggtaaactg     2220

tacctgttcc agatctacaa taaagatttt gccaaaggcc atcatggcaa accgaatctg     2280

cataccctgt attggaccgg tctgtttagc cctgaaaatc tggcaaaaac ctcgattaaa     2340

ctgaatggtc aggcggaact gttttatcgt ccgaaaagcc gtatgaaacg tatggcacat     2400

cgtctgggtg aaaaaatgct gaacaaaaaa ctgaaagacc agaaaacccc gatcccggat     2460

acactgtatc aagaactgta tgattatgtg aaccatcgtc tgagccatga tctgagtgat     2520

gaagcacgtg ccctgctgcc gaatgttatt accaaagaag ttagccacga gatcattaaa     2580

gatcgtcgtt ttaccagcga caaattcctg tttcatgtgc cgattaccct gaattatcag     2640

gcagcaaata gcccgagcaa atttaaccag cgtgttaatg catatctgaa agaacatcca     2700

gaaacgccga ttattggtat tgatcgtggt gaacgtaacc tgatttatat caccgttatt     2760

gatagcaccg gcaaaatcct ggaacagcgt agcctgaata ccattcagca gtttgattac     2820

cagaaaaaac tggataatcg cgagaaagaa cgtgttgcag cacgtcaggc atggtcagtt     2880

gttggtacaa ttaaagacct gaaacagggt tatctgagcc aggttattca tgaaattgtg     2940

gatctgatga ttcactatca ggccgttgtt gtgctggaaa acctgaattt tggctttaaa     3000

agcaaacgta ccggcattgc agaaaaagca gtttatcagc agttcgagaa aatgctgatt     3060

gacaaactga attgcctggt gctgaaagat tatccggctg aaaaagttgg tggtgttctg     3120

aatccgtatc agctgaccga tcagtttacc agctttgcaa aaatgggcac ccagagcgga     3180

tttctgtttt atgttccggc accgtatacg agcaaaattg atccgctgac cggttttgtt     3240

gatccgtttg tttggaaaac catcaaaaac catgaaagcc gcaaacattt tctggaaggt     3300

ttcgattttc tgcattacga cgttaaaacg ggtgatttca tcctgcactt taaaatgaat     3360

cgcaatctga gttttcagcg tggcctgcct ggttttatgc ctgcatggga tattgtgttt     3420

gagaaaaacg aaacacagtt cgatgcaaaa ggcaccccgt ttattgcagg taaacgtatt     3480

gttccggtga ttgaaaatca tcgtttcacc ggtcgttatc gcgatctgta tccggcaaat     3540

gaactgatcg cactgctgga agagaaaggt attgtttttc gtgatggctc aaacattctg     3600

ccgaaactgc tggaaaatga tgatagccat gcaattgata ccatggttgc actgattcgt     3660

agcgttctgc agatgcgtaa tagcaatgca gcaaccggtg aagattacat taatagtccg     3720

gttcgtgatc tgaatggtgt ttgttttgat agccgttttc agaatccgga atggccgatg     3780

gatgcagatg caaatggtgc atatcatatt gcactgaaag gacagctgct gctgaaccac     3840

ctgaaagaaa gcaaagatct gaaactgcaa aacggcatta gcaatcagga ttggctggca     3900

tatatccaag aactgcgtaa cggtcgtagc agtgatgatg aagcaaccgc agatagccag     3960

catgcagcac cgcctaaaaa gaaacgtaaa gttggtggta gcggtggttc aggtggtagt     4020

ggcggtagtg gtggctcagg gggttctggt ggctctggtg gtagcctcga gcaccaccac     4080

caccaccac                                                             4089


<210>  1112
<211>  4089
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  misc_feature
<222>  (3928)..(3993)
<223>  NLS

<220>
<221>  misc_feature
<222>  (4072)..(4089)
<223>  His tag

<400>  1112
atgacccagt ttgaaggttt caccaatctg tatcaggtta gcaaaaccct gcgttttgaa       60

ctgattccgc agggtaaaac cctgaaacat attcaagaac agggcttcat cgaagaggat      120

aaagcacgta acgatcacta caaagaactg aaaccgatta tcgaccgcat ctataaaacc      180

tatgcagatc agtgtctgca gctggttcag ctggattggg aaaatctgag cgcagcaatt      240

gatagttatc gcaaagaaaa aaccgaagaa acccgtaatg cactgattga agaacaggca      300

acctatcgta atgccatcca tgattatttc attggtcgta ccgataatct gaccgatgca      360

attaacaaac gtcacgccga aatctataaa ggcctgttta aagccgaact gtttaatggc      420

aaagttctga aacagctggg caccgttacc accaccgaac atgaaaatgc actgctgcgt      480

agctttgata aattcaccac ctatttcagc ggcttttatg agaatcgcaa aaacgtgttt      540

agcgcagaag atattagcac cgcaattccg catcgtattg tgcaggataa tttcccgaaa      600

ttcaaagaga actgccacat ttttacccgt ctgattaccg cagttccgag cctgcgtgaa      660

cattttgaaa acgttaaaaa agccatcggc atctttgtta gcaccagcat tgaagaagtt      720

tttagcttcc cgttttacaa tcagctgctg acccagaccc agattgatct gtataaccaa      780

ctgctgggtg gtattagccg tgaagcaggc accgaaaaaa tcaaaggtct gaatgaagtg      840

ctgaatctgg ccattcagaa aaatgatgaa accgcacata ttattgcaag cctgccgcat      900

cgttttattc cgctgttcaa acaaattctg agcgatcgta ataccctgag ctttattctg      960

gaagaattca aatccgatga agaggtgatt cagagctttt gcaaatacaa aacgctgctg     1020

cgcaatgaaa atgttctgga aactgccgaa gcactgttta acgaactgaa tagcattgat     1080

ctgacccaca tctttatcag ccacaaaaaa ctggaaacca tttcaagcgc actgtgtgat     1140

cattgggata ccctgcgtaa tgccctgtat gaacgtcgta ttagcgaact gaccggtaaa     1200

attaccaaaa gcgcgaaaga aaaagttcag cgcagtctga aacatgagga tattaatctg     1260

caagagatta ttagcgcagc cggtaaagaa ctgtcagaag catttaaaca gaaaaccagc     1320

gaaattctgt cacatgcaca tgcagcactg gatcagccgc tgccgaccac cctgaaaaaa     1380

caagaagaaa aagaaatcct gaaaagccag ctggatagcc tgctgggtct gtatcatctg     1440

ctggactggt ttgcagttga tgaaagcaat gaagttgatc cggaatttag cgcacgtctg     1500

accggcatta aactggaaat ggaaccgagc ctgagctttt ataacaaagc ccgtaattat     1560

gccaccaaaa aaccgtatag cgtcgaaaaa ttcaaactga actttcagat gccgaccctg     1620

gcaagcggtt gggatgttaa taaagaaaaa aacaacggtg ccatcctgtt cgtgaaaaat     1680

ggcctgtatt atctgggtat tatgccgaaa cagaaaggtc gttataaagc gctgagcttt     1740

gaaccgacgg aaaaaaccag tgaaggtttt gataaaatgt actacgacta ttttccggat     1800

gcagccaaaa tgattccgaa atgtagcacc cagctgaaag cagttaccgc acattttcag     1860

acccatacca ccccgattct gctgagcaat aactttattg aaccgctgga aatcaccaaa     1920

gagatctacg atctgaataa cccggaaaaa gagccgaaaa aattccagac cgcatatgca     1980

aaaaaaaccg gtgatcagaa aggttatcgt gaagcgctgt gtaaatggat tgatttcacc     2040

cgtgattttc tgagcaaata caccaaaacc accagtatcg atctgagcag cctgcgtccg     2100

agcagccagt ataaagatct gggcgaatat tatgcagaac tgaatccgct gctgtatcat     2160

attagctttc agcgtattgc cgagaaagaa atcatggacg cagttgaaac cggtaaactg     2220

tacctgttcc agatctacaa taaagatttt gccaaaggcc atcatggcaa accgaatctg     2280

cataccctgt attggaccgg tctgtttagc cctgaaaatc tggcaaaaac ctcgattaaa     2340

ctgaatggtc aggcggaact gttttatcgt ccgaaaagcc gtatgaaacg tatggcacat     2400

cgtctgggtg aaaaaatgct gaacaaaaaa ctgaaagacc agaaaacccc gatcccggat     2460

acactgtatc aagaactgta tgattatgtg aaccatcgtc tgagccatga tctgagtgat     2520

gaagcacgtg ccctgctgcc gaatgttatt accaaagaag ttagccacga gatcattaaa     2580

gatcgtcgtt ttaccagcga caaattcttt tttcatgtgc cgattaccct gaattatcag     2640

gcagcaaata gcccgagcaa atttaaccag cgtgttaatg catatctgaa agaacatcca     2700

gaaacgccga ttattggtat tgatcgtggt gaacgtaacc tgatttatat caccgttatt     2760

gatagcaccg gcaaaatcct ggaacagcgt agcctgaata ccattcagca gtttgattac     2820

cagaaaaaac tggataatcg cgagaaagaa cgtgttgcag cacgtcaggc atggtcagtt     2880

gttggtacaa ttaaagacct gaaacagggt tatctgagcc aggttattca tgaaattgtg     2940

gatctgatga ttcactatca ggccgttgtt gtgctggaaa acctgaattt tggctttaaa     3000

agcaaacgta ccggcattgc agaaaaagca gtttatcagc agttcgagaa aatgctgatt     3060

gacaaactga attgcctggt gctgaaagat tatccggctg aaaaagttgg tggtgttctg     3120

aatccgtatc agctgaccga tcagtttacc agctttgcaa aaatgggcac ccagagcgga     3180

tttctgtttt atgttccggc accgtatacg agcaaaattg atccgctgac cggttttgtt     3240

gatccgtttg tttggaaaac catcaaaaac catgaaagcc gcaaacattt tctggaaggt     3300

ttcgattttc tgcattacga cgttaaaacg ggtgatttca tcctgcactt taaaatgaat     3360

cgcaatctga gttttcagcg tggcctgcct ggttttatgc ctgcatggga tattgtgttt     3420

gagaaaaacg aaacacagtt cgatgcaaaa ggcaccccgt ttattgcagg taaacgtatt     3480

gttccggtga ttgaaaatca tcgtttcacc ggtcgttatc gcgatctgta tccggcaaat     3540

gaactgatcg cactgctgga agagaaaggt attgtttttc gtgatggctc aaacattctg     3600

ccgaaactgc tggaaaatga tgatagccat gcaattgata ccatggttgc actgattcgt     3660

agcgttctgc agatgcgtaa tagcaatgca gcaaccggtg aagattacat taatagtccg     3720

gttcgtgatc tgaatggtgt ttgttttgat agccgttttc agaatccgga atggccgatg     3780

gatgcagatg caaatggtgc atatcatatt gcactgaaag gacagctgct gctgaaccac     3840

ctgaaagaaa gcaaagatct gaaactgcaa aacggcatta gcaatcagga ttggctggca     3900

tatatccaag aactgcgtaa cggtcgtagc agtgatgatg aagcaaccgc agatagccag     3960

catgcagcac cgcctaaaaa gaaacgtaaa gttggtggta gcggtggttc aggtggtagt     4020

ggcggtagtg gtggctcagg gggttctggt ggctctggtg gtagcctcga gcaccaccac     4080

caccaccac                                                             4089


<210>  1113
<211>  4089
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  misc_feature
<222>  (3928)..(3993)
<223>  NLS

<220>
<221>  misc_feature
<222>  (4072)..(4089)
<223>  His tag

<400>  1113
atgacccagt ttgaaggttt caccaatctg tatcaggtta gcaaaaccct gcgttttgaa       60

ctgattccgc agggtaaaac cctgaaacat attcaagaac agggcttcat cgaagaggat      120

aaagcacgta acgatcacta caaagaactg aaaccgatta tcgaccgcat ctataaaacc      180

tatgcagatc agtgtctgca gctggttcag ctggattggg aaaatctgag cgcagcaatt      240

gatagttatc gcaaagaaaa aaccgaagaa acccgtaatg cactgattga agaacaggca      300

acctatcgta atgccatcca tgattatttc attggtcgta ccgataatct gaccgatgca      360

attaacaaac gtcacgccga aatctataaa ggcctgttta aagccgaact gtttaatggc      420

aaagttctga aacagctggg caccgttacc accaccgaac atgaaaatgc actgctgcgt      480

agctttgata aattcaccac ctatttcagc ggcttttatg agaatcgcaa aaacgtgttt      540

agcgcagaag atattagcac cgcaattccg catcgtattg tgcaggataa tttcccgaaa      600

ttcaaagaga actgccacat ttttacccgt ctgattaccg cagttccgag cctgcgtgaa      660

cattttgaaa acgttaaaaa agccatcggc atctttgtta gcaccagcat tgaagaagtt      720

tttagcttcc cgttttacaa tcagctgctg acccagaccc agattgatct gtataaccaa      780

ctgctgggtg gtattagccg tgaagcaggc accgaaaaaa tcaaaggtct gaatgaagtg      840

ctgaatctgg ccattcagaa aaatgatgaa accgcacata ttattgcaag cctgccgcat      900

cgttttattc cgctgttcaa acaaattctg agcgatcgta ataccctgag ctttattctg      960

gaagaattca aatccgatga agaggtgatt cagagctttt gcaaatacaa aacgctgctg     1020

cgcaatgaaa atgttctgga aactgccgaa gcactgttta acgaactgaa tagcattgat     1080

ctgacccaca tctttatcag ccacaaaaaa ctggaaacca tttcaagcgc actgtgtgat     1140

cattgggata ccctgcgtaa tgccctgtat gaacgtcgta ttagcgaact gaccggtaaa     1200

attaccaaaa gcgcgaaaga aaaagttcag cgcagtctga aacatgagga tattaatctg     1260

caagagatta ttagcgcagc cggtaaagaa ctgtcagaag catttaaaca gaaaaccagc     1320

gaaattctgt cacatgcaca tgcagcactg gatcagccgc tgccgaccac cctgaaaaaa     1380

caagaagaaa aagaaatcct gaaaagccag ctggatagcc tgctgggtct gtatcatctg     1440

ctggactggt ttgcagttga tgaaagcaat gaagttgatc cggaatttag cgcacgtctg     1500

accggcatta aactggaaat ggaaccgagc ctgagctttt ataacaaagc ccgtaattat     1560

gccaccaaaa aaccgtatag cgtcgaaaaa ttcaaactga actttcagcg tccgaccctg     1620

gcaagcggtt gggatgttaa taaagaaaaa aacaacggtg ccatcctgtt cgtgaaaaat     1680

ggcctgtatt atctgggtat tatgccgaaa cagaaaggtc gttataaagc gctgagcttt     1740

gaaccgacgg aaaaaaccag tgaaggtttt gataaaatgt actacgacta ttttccggat     1800

gcagccaaaa tgattccgaa atgtagcacc cagctgaaag cagttaccgc acattttcag     1860

acccatacca ccccgattct gctgagcaat aactttattg aaccgctgga aatcaccaaa     1920

gagatctacg atctgaataa cccggaaaaa gagccgaaaa aattccagac cgcatatgca     1980

aaaaaaaccg gtgatcagaa aggttatcgt gaagcgctgt gtaaatggat tgatttcacc     2040

cgtgattttc tgagcaaata caccaaaacc accagtatcg atctgagcag cctgcgtccg     2100

agcagccagt ataaagatct gggcgaatat tatgcagaac tgaatccgct gctgtatcat     2160

attagctttc agcgtattgc cgagaaagaa atcatggacg cagttgaaac cggtaaactg     2220

tacctgttcc agatctacaa taaagatttt gccaaaggcc atcatggcaa accgaatctg     2280

cataccctgt attggaccgg tctgtttagc cctgaaaatc tggcaaaaac ctcgattaaa     2340

ctgaatggtc aggcggaact gttttatcgt ccgaaaagcc gtatgaaacg tatggcagct     2400

cgtctgggtg aaaaaatgct gaacaaaaaa ctgaaagacc agaaaacccc gatcccggat     2460

acactgtatc aagaactgta tgattatgtg aaccatcgtc tgagccatga tctgagtgat     2520

gaagcacgtg ccctgctgcc gaatgttatt accaaagaag ttagccacga gatcattaaa     2580

gatcgtcgtt ttaccagcga caaattcctg tttcatgtgc cgattaccct gaattatcag     2640

gcagcaaata gcccgagcaa atttaaccag cgtgttaatg catatctgaa agaacatcca     2700

gaaacgccga ttattggtat tgatcgtggt gaacgtaacc tgatttatat caccgttatt     2760

gatagcaccg gcaaaatcct ggaacagcgt agcctgaata ccattcagca gtttgattac     2820

cagaaaaaac tggataatcg cgagaaagaa cgtgttgcag cacgtcaggc atggtcagtt     2880

gttggtacaa ttaaagacct gaaacagggt tatctgagcc aggttattca tgaaattgtg     2940

gatctgatga ttcactatca ggccgttgtt gtgctggaaa acctgaattt tggctttaaa     3000

agcaaacgta ccggcattgc agaaaaagca gtttatcagc agttcgagaa aatgctgatt     3060

gacaaactga attgcctggt gctgaaagat tatccggctg aaaaagttgg tggtgttctg     3120

aatccgtatc agctgaccga tcagtttacc agctttgcaa aaatgggcac ccagagcgga     3180

tttctgtttt atgttccggc accgtatacg agcaaaattg atccgctgac cggttttgtt     3240

gatccgtttg tttggaaaac catcaaaaac catgaaagcc gcaaacattt tctggaaggt     3300

ttcgattttc tgcattacga cgttaaaacg ggtgatttca tcctgcactt taaaatgaat     3360

cgcaatctga gttttcagcg tggcctgcct ggttttatgc ctgcatggga tattgtgttt     3420

gagaaaaacg aaacacagtt cgatgcaaaa ggcaccccgt ttattgcagg taaacgtatt     3480

gttccggtga ttgaaaatca tcgtttcacc ggtcgttatc gcgatctgta tccggcaaat     3540

gaactgatcg cactgctgga agagaaaggt attgtttttc gtgatggctc aaacattctg     3600

ccgaaactgc tggaaaatga tgatagccat gcaattgata ccatggttgc actgattcgt     3660

agcgttctgc agatgcgtaa tagcaatgca gcaaccggtg aagattacat taatagtccg     3720

gttcgtgatc tgaatggtgt ttgttttgat agccgttttc agaatccgga atggccgatg     3780

gatgcagatg caaatggtgc atatcatatt gcactgaaag gacagctgct gctgaaccac     3840

ctgaaagaaa gcaaagatct gaaactgcaa aacggcatta gcaatcagga ttggctggca     3900

tatatccaag aactgcgtaa cggtcgtagc agtgatgatg aagcaaccgc agatagccag     3960

catgcagcac cgcctaaaaa gaaacgtaaa gttggtggta gcggtggttc aggtggtagt     4020

ggcggtagtg gtggctcagg gggttctggt ggctctggtg gtagcctcga gcaccaccac     4080

caccaccac                                                             4089


<210>  1114
<211>  3993
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  misc_feature
<222>  (3928)..(3993)
<223>  NLS

<400>  1114
atgacccagt ttgaaggttt caccaatctg tatcaggtta gcaaaaccct gcgttttgaa       60

ctgattccgc agggtaaaac cctgaaacat attcaagaac agggcttcat cgaagaggat      120

aaagcacgta acgatcacta caaagaactg aaaccgatta tcgaccgcat ctataaaacc      180

tatgcagatc agtgtctgca gctggttcag ctggattggg aaaatctgag cgcagcaatt      240

gatagttatc gcaaagaaaa aaccgaagaa acccgtaatg cactgattga agaacaggca      300

acctatcgta atgccatcca tgattatttc attggtcgta ccgataatct gaccgatgca      360

attaacaaac gtcacgccga aatctataaa ggcctgttta aagccgaact gtttaatggc      420

aaagttctga aacagctggg caccgttacc accaccgaac atgaaaatgc actgctgcgt      480

agctttgata aattcaccac ctatttcagc ggcttttatg agaatcgcaa aaacgtgttt      540

agcgcagaag atattagcac cgcaattccg catcgtattg tgcaggataa tttcccgaaa      600

ttcaaagaga actgccacat ttttacccgt ctgattaccg cagttccgag cctgcgtgaa      660

cattttgaaa acgttaaaaa agccatcggc atctttgtta gcaccagcat tgaagaagtt      720

tttagcttcc cgttttacaa tcagctgctg acccagaccc agattgatct gtataaccaa      780

ctgctgggtg gtattagccg tgaagcaggc accgaaaaaa tcaaaggtct gaatgaagtg      840

ctgaatctgg ccattcagaa aaatgatgaa accgcacata ttattgcaag cctgccgcat      900

cgttttattc cgctgttcaa acaaattctg agcgatcgta ataccctgag ctttattctg      960

gaagaattca aatccgatga agaggtgatt cagagctttt gcaaatacaa aacgctgctg     1020

cgcaatgaaa atgttctgga aactgccgaa gcactgttta acgaactgaa tagcattgat     1080

ctgacccaca tctttatcag ccacaaaaaa ctggaaacca tttcaagcgc actgtgtgat     1140

cattgggata ccctgcgtaa tgccctgtat gaacgtcgta ttagcgaact gaccggtaaa     1200

attaccaaaa gcgcgaaaga aaaagttcag cgcagtctga aacatgagga tattaatctg     1260

caagagatta ttagcgcagc cggtaaagaa ctgtcagaag catttaaaca gaaaaccagc     1320

gaaattctgt cacatgcaca tgcagcactg gatcagccgc tgccgaccac cctgaaaaaa     1380

caagaagaaa aagaaatcct gaaaagccag ctggatagcc tgctgggtct gtatcatctg     1440

ctggactggt ttgcagttga tgaaagcaat gaagttgatc cggaatttag cgcacgtctg     1500

accggcatta aactggaaat ggaaccgagc ctgagctttt ataacaaagc ccgtaattat     1560

gccaccaaaa aaccgtatag cgtcgaaaaa ttcaaactga actttcagcg tccgaccctg     1620

gcaagcggtt gggatgttaa taaagaaaaa aacaacggtg ccatcctgtt cgtgaaaaat     1680

ggcctgtatt atctgggtat tatgccgaaa cagaaaggtc gttataaagc gctgagcttt     1740

gaaccgacgg aaaaaaccag tgaaggtttt gataaaatgt actacgacta ttttccggat     1800

gcagccaaaa tgattccgaa atgtagcacc cagctgaaag cagttaccgc acattttcag     1860

acccatacca ccccgattct gctgagcaat aactttattg aaccgctgga aatcaccaaa     1920

gagatctacg atctgaataa cccggaaaaa gagccgaaaa aattccagac cgcatatgca     1980

aaaaaaaccg gtgatcagaa aggttatcgt gaagcgctgt gtaaatggat tgatttcacc     2040

cgtgattttc tgagcaaata caccaaaacc accagtatcg atctgagcag cctgcgtccg     2100

agcagccagt ataaagatct gggcgaatat tatgcagaac tgaatccgct gctgtatcat     2160

attagctttc agcgtattgc cgagaaagaa atcatggacg cagttgaaac cggtaaactg     2220

tacctgttcc agatctacaa taaagatttt gccaaaggcc atcatggcaa accgaatctg     2280

cataccctgt attggaccgg tctgtttagc cctgaaaatc tggcaaaaac ctcgattaaa     2340

ctgaatggtc aggcggaact gttttatcgt ccgaaaagcc gtatgaaacg tatggcagct     2400

cgtctgggtg aaaaaatgct gaacaaaaaa ctgaaagacc agaaaacccc gatcccggat     2460

acactgtatc aagaactgta tgattatgtg aaccatcgtc tgagccatga tctgagtgat     2520

gaagcacgtg ccctgctgcc gaatgttatt accaaagaag ttagccacga gatcattaaa     2580

gatcgtcgtt ttaccagcga caaattcctg tttcatgtgc cgattaccct gaattatcag     2640

gcagcaaata gcccgagcaa atttaaccag cgtgttaatg catatctgaa agaacatcca     2700

gaaacgccga ttattggtat tgatcgtggt gaacgtaacc tgatttatat caccgttatt     2760

gatagcaccg gcaaaatcct ggaacagcgt agcctgaata ccattcagca gtttgattac     2820

cagaaaaaac tggataatcg cgagaaagaa cgtgttgcag cacgtcaggc atggtcagtt     2880

gttggtacaa ttaaagacct gaaacagggt tatctgagcc aggttattca tgaaattgtg     2940

gatctgatga ttcactatca ggccgttgtt gtgctggaaa acctgaattt tggctttaaa     3000

agcaaacgta ccggcattgc agaaaaagca gtttatcagc agttcgagaa aatgctgatt     3060

gacaaactga attgcctggt gctgaaagat tatccggctg aaaaagttgg tggtgttctg     3120

aatccgtatc agctgaccga tcagtttacc agctttgcaa aaatgggcac ccagagcgga     3180

tttctgtttt atgttccggc accgtatacg agcaaaattg atccgctgac cggttttgtt     3240

gatccgtttg tttggaaaac catcaaaaac catgaaagcc gcaaacattt tctggaaggt     3300

ttcgattttc tgcattacga cgttaaaacg ggtgatttca tcctgcactt taaaatgaat     3360

cgcaatctga gttttcagcg tggcctgcct ggttttatgc ctgcatggga tattgtgttt     3420

gagaaaaacg aaacacagtt cgatgcaaaa ggcaccccgt ttattgcagg taaacgtatt     3480

gttccggtga ttgaaaatca tcgtttcacc ggtcgttatc gcgatctgta tccggcaaat     3540

gaactgatcg cactgctgga agagaaaggt attgtttttc gtgatggctc aaacattctg     3600

ccgaaactgc tggaaaatga tgatagccat gcaattgata ccatggttgc actgattcgt     3660

agcgttctgc agatgcgtaa tagcaatgca gcaaccggtg aagattacat taatagtccg     3720

gttcgtgatc tgaatggtgt ttgttttgat agccgttttc agaatccgga atggccgatg     3780

gatgcagatg caaatggtgc atatcatatt gcactgaaag gacagctgct gctgaaccac     3840

ctgaaagaaa gcaaagatct gaaactgcaa aacggcatta gcaatcagga ttggctggca     3900

tatatccaag aactgcgtaa cggtcgtagc agtgatgatg aagcaaccgc agatagccag     3960

catgcagcac cgcctaaaaa gaaacgtaaa gtt                                  3993


<210>  1115
<211>  3993
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  misc_feature
<222>  (3928)..(3993)
<223>  NLS

<400>  1115
agcacccagt ttgaaggttt caccaatctg tatcaggtta gcaaaaccct gcgttttgaa       60

ctgattccgc agggtaaaac cctgaaacat attcaagaac agggcttcat cgaagaggat      120

aaagcacgta acgatcacta caaagaactg aaaccgatta tcgaccgcat ctataaaacc      180

tatgcagatc agtgtctgca gctggttcag ctggattggg aaaatctgag cgcagcaatt      240

gatagttatc gcaaagaaaa aaccgaagaa acccgtaatg cactgattga agaacaggca      300

acctatcgta atgccatcca tgattatttc attggtcgta ccgataatct gaccgatgca      360

attaacaaac gtcacgccga aatctataaa ggcctgttta aagccgaact gtttaatggc      420

aaagttctga aacagctggg caccgttacc accaccgaac atgaaaatgc actgctgcgt      480

agctttgata aattcaccac ctatttcagc ggcttttatg agaatcgcaa aaacgtgttt      540

agcgcagaag atattagcac cgcaattccg catcgtattg tgcaggataa tttcccgaaa      600

ttcaaagaga actgccacat ttttacccgt ctgattaccg cagttccgag cctgcgtgaa      660

cattttgaaa acgttaaaaa agccatcggc atctttgtta gcaccagcat tgaagaagtt      720

tttagcttcc cgttttacaa tcagctgctg acccagaccc agattgatct gtataaccaa      780

ctgctgggtg gtattagccg tgaagcaggc accgaaaaaa tcaaaggtct gaatgaagtg      840

ctgaatctgg ccattcagaa aaatgatgaa accgcacata ttattgcaag cctgccgcat      900

cgttttattc cgctgttcaa acaaattctg agcgatcgta ataccctgag ctttattctg      960

gaagaattca aatccgatga agaggtgatt cagagctttt gcaaatacaa aacgctgctg     1020

cgcaatgaaa atgttctgga aactgccgaa gcactgttta acgaactgaa tagcattgat     1080

ctgacccaca tctttatcag ccacaaaaaa ctggaaacca tttcaagcgc actgtgtgat     1140

cattgggata ccctgcgtaa tgccctgtat gaacgtcgta ttagcgaact gaccggtaaa     1200

attaccaaaa gcgcgaaaga aaaagttcag cgcagtctga aacatgagga tattaatctg     1260

caagagatta ttagcgcagc cggtaaagaa ctgtcagaag catttaaaca gaaaaccagc     1320

gaaattctgt cacatgcaca tgcagcactg gatcagccgc tgccgaccac cctgaaaaaa     1380

caagaagaaa aagaaatcct gaaaagccag ctggatagcc tgctgggtct gtatcatctg     1440

ctggactggt ttgcagttga tgaaagcaat gaagttgatc cggaatttag cgcacgtctg     1500

accggcatta aactggaaat ggaaccgagc ctgagctttt ataacaaagc ccgtaattat     1560

gccaccaaaa aaccgtatag cgtcgaaaaa ttcaaactga actttcagcg tccgaccctg     1620

gcaagcggtt gggatgttaa taaagaaaaa aacaacggtg ccatcctgtt cgtgaaaaat     1680

ggcctgtatt atctgggtat tatgccgaaa cagaaaggtc gttataaagc gctgagcttt     1740

gaaccgacgg aaaaaaccag tgaaggtttt gataaaatgt actacgacta ttttccggat     1800

gcagccaaaa tgattccgaa atgtagcacc cagctgaaag cagttaccgc acattttcag     1860

acccatacca ccccgattct gctgagcaat aactttattg aaccgctgga aatcaccaaa     1920

gagatctacg atctgaataa cccggaaaaa gagccgaaaa aattccagac cgcatatgca     1980

aaaaaaaccg gtgatcagaa aggttatcgt gaagcgctgt gtaaatggat tgatttcacc     2040

cgtgattttc tgagcaaata caccaaaacc accagtatcg atctgagcag cctgcgtccg     2100

agcagccagt ataaagatct gggcgaatat tatgcagaac tgaatccgct gctgtatcat     2160

attagctttc agcgtattgc cgagaaagaa atcatggacg cagttgaaac cggtaaactg     2220

tacctgttcc agatctacaa taaagatttt gccaaaggcc atcatggcaa accgaatctg     2280

cataccctgt attggaccgg tctgtttagc cctgaaaatc tggcaaaaac ctcgattaaa     2340

ctgaatggtc aggcggaact gttttatcgt ccgaaaagcc gtatgaaacg tatggcacat     2400

cgtctgggtg aaaaaatgct gaacaaaaaa ctgaaagacc agaaaacccc gatcccggat     2460

acactgtatc aagaactgta tgattatgtg aaccatcgtc tgagccatga tctgagtgat     2520

gaagcacgtg ccctgctgcc gaatgttatt accaaagaag ttagccacga gatcattaaa     2580

gatcgtcgtt ttaccagcga caaattcctg tttcatgtgc cgattaccct gaattatcag     2640

gcagcaaata gcccgagcaa atttaaccag cgtgttaatg catatctgaa agaacatcca     2700

gaaacgccga ttattggtat tgatcgtggt gaacgtaacc tgatttatat caccgttatt     2760

gatagcaccg gcaaaatcct ggaacagcgt agcctgaata ccattcagca gtttgattac     2820

cagaaaaaac tggataatcg cgagaaagaa cgtgttgcag cacgtcaggc atggtcagtt     2880

gttggtacaa ttaaagacct gaaacagggt tatctgagcc aggttattca tgaaattgtg     2940

gatctgatga ttcactatca ggccgttgtt gtgctggaaa acctgaattt tggctttaaa     3000

agcaaacgta ccggcattgc agaaaaagca gtttatcagc agttcgagaa aatgctgatt     3060

gacaaactga attgcctggt gctgaaagat tatccggctg aaaaagttgg tggtgttctg     3120

aatccgtatc agctgaccga tcagtttacc agctttgcaa aaatgggcac ccagagcgga     3180

tttctgtttt atgttccggc accgtatacg agcaaaattg atccgctgac cggttttgtt     3240

gatccgtttg tttggaaaac catcaaaaac catgaaagcc gcaaacattt tctggaaggt     3300

ttcgattttc tgcattacga cgttaaaacg ggtgatttca tcctgcactt taaaatgaat     3360

cgcaatctga gttttcagcg tggcctgcct ggttttatgc ctgcatggga tattgtgttt     3420

gagaaaaacg aaacacagtt cgatgcaaaa ggcaccccgt ttattgcagg taaacgtatt     3480

gttccggtga ttgaaaatca tcgtttcacc ggtcgttatc gcgatctgta tccggcaaat     3540

gaactgatcg cactgctgga agagaaaggt attgtttttc gtgatggctc aaacattctg     3600

ccgaaactgc tggaaaatga tgatagccat gcaattgata ccatggttgc actgattcgt     3660

agcgttctgc agatgcgtaa tagcaatgca gcaaccggtg aagattacat taatagtccg     3720

gttcgtgatc tgaatggtgt ttgttttgat agccgttttc agaatccgga atggccgatg     3780

gatgcagatg caaatggtgc atatcatatt gcactgaaag gacagctgct gctgaaccac     3840

ctgaaagaaa gcaaagatct gaaactgcaa aacggcatta gcaatcagga ttggctggca     3900

tatatccaag aactgcgtaa cggtcgtagc agtgatgatg aagcaaccgc agatagccag     3960

catgcagcac cgcctaaaaa gaaacgtaaa gtt                                  3993


<210>  1116
<211>  4089
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  misc_feature
<222>  (3928)..(3993)
<223>  NLS

<220>
<221>  misc_feature
<222>  (4072)..(4089)
<223>  His tag

<400>  1116
agcacccagt ttgaaggttt caccaatctg tatcaggtta gcaaaaccct gcgttttgaa       60

ctgattccgc agggtaaaac cctgaaacat attcaagaac agggcttcat cgaagaggat      120

aaagcacgta acgatcacta caaagaactg aaaccgatta tcgaccgcat ctataaaacc      180

tatgcagatc agtgtctgca gctggttcag ctggattggg aaaatctgag cgcagcaatt      240

gatagttatc gcaaagaaaa aaccgaagaa acccgtaatg cactgattga agaacaggca      300

acctatcgta atgccatcca tgattatttc attggtcgta ccgataatct gaccgatgca      360

attaacaaac gtcacgccga aatctataaa ggcctgttta aagccgaact gtttaatggc      420

aaagttctga aacagctggg caccgttacc accaccgaac atgaaaatgc actgctgcgt      480

agctttgata aattcaccac ctatttcagc ggcttttatg agaatcgcaa aaacgtgttt      540

agcgcagaag atattagcac cgcaattccg catcgtattg tgcaggataa tttcccgaaa      600

ttcaaagaga actgccacat ttttacccgt ctgattaccg cagttccgag cctgcgtgaa      660

cattttgaaa acgttaaaaa agccatcggc atctttgtta gcaccagcat tgaagaagtt      720

tttagcttcc cgttttacaa tcagctgctg acccagaccc agattgatct gtataaccaa      780

ctgctgggtg gtattagccg tgaagcaggc accgaaaaaa tcaaaggtct gaatgaagtg      840

ctgaatctgg ccattcagaa aaatgatgaa accgcacata ttattgcaag cctgccgcat      900

cgttttattc cgctgttcaa acaaattctg agcgatcgta ataccctgag ctttattctg      960

gaagaattca aatccgatga agaggtgatt cagagctttt gcaaatacaa aacgctgctg     1020

cgcaatgaaa atgttctgga aactgccgaa gcactgttta acgaactgaa tagcattgat     1080

ctgacccaca tctttatcag ccacaaaaaa ctggaaacca tttcaagcgc actgtgtgat     1140

cattgggata ccctgcgtaa tgccctgtat gaacgtcgta ttagcgaact gaccggtaaa     1200

attaccaaaa gcgcgaaaga aaaagttcag cgcagtctga aacatgagga tattaatctg     1260

caagagatta ttagcgcagc cggtaaagaa ctgtcagaag catttaaaca gaaaaccagc     1320

gaaattctgt cacatgcaca tgcagcactg gatcagccgc tgccgaccac cctgaaaaaa     1380

caagaagaaa aagaaatcct gaaaagccag ctggatagcc tgctgggtct gtatcatctg     1440

ctggactggt ttgcagttga tgaaagcaat gaagttgatc cggaatttag cgcacgtctg     1500

accggcatta aactggaaat ggaaccgagc ctgagctttt ataacaaagc ccgtaattat     1560

gccaccaaaa aaccgtatag cgtcgaaaaa ttcaaactga actttcagcg tccgaccctg     1620

gcaagcggtt gggatgttaa taaagaaaaa aacaacggtg ccatcctgtt cgtgaaaaat     1680

ggcctgtatt atctgggtat tatgccgaaa cagaaaggtc gttataaagc gctgagcttt     1740

gaaccgacgg aaaaaaccag tgaaggtttt gataaaatgt actacgacta ttttccggat     1800

gcagccaaaa tgattccgaa atgtagcacc cagctgaaag cagttaccgc acattttcag     1860

acccatacca ccccgattct gctgagcaat aactttattg aaccgctgga aatcaccaaa     1920

gagatctacg atctgaataa cccggaaaaa gagccgaaaa aattccagac cgcatatgca     1980

aaaaaaaccg gtgatcagaa aggttatcgt gaagcgctgt gtaaatggat tgatttcacc     2040

cgtgattttc tgagcaaata caccaaaacc accagtatcg atctgagcag cctgcgtccg     2100

agcagccagt ataaagatct gggcgaatat tatgcagaac tgaatccgct gctgtatcat     2160

attagctttc agcgtattgc cgagaaagaa atcatggacg cagttgaaac cggtaaactg     2220

tacctgttcc agatctacaa taaagatttt gccaaaggcc atcatggcaa accgaatctg     2280

cataccctgt attggaccgg tctgtttagc cctgaaaatc tggcaaaaac ctcgattaaa     2340

ctgaatggtc aggcggaact gttttatcgt ccgaaaagcc gtatgaaacg tatggcacat     2400

cgtctgggtg aaaaaatgct gaacaaaaaa ctgaaagacc agaaaacccc gatcccggat     2460

acactgtatc aagaactgta tgattatgtg aaccatcgtc tgagccatga tctgagtgat     2520

gaagcacgtg ccctgctgcc gaatgttatt accaaagaag ttagccacga gatcattaaa     2580

gatcgtcgtt ttaccagcga caaattcctg tttcatgtgc cgattaccct gaattatcag     2640

gcagcaaata gcccgagcaa atttaaccag cgtgttaatg catatctgaa agaacatcca     2700

gaaacgccga ttattggtat tgatcgtggt gaacgtaacc tgatttatat caccgttatt     2760

gatagcaccg gcaaaatcct ggaacagcgt agcctgaata ccattcagca gtttgattac     2820

cagaaaaaac tggataatcg cgagaaagaa cgtgttgcag cacgtcaggc atggtcagtt     2880

gttggtacaa ttaaagacct gaaacagggt tatctgagcc aggttattca tgaaattgtg     2940

gatctgatga ttcactatca ggccgttgtt gtgctggaaa acctgaattt tggctttaaa     3000

agcaaacgta ccggcattgc agaaaaagca gtttatcagc agttcgagaa aatgctgatt     3060

gacaaactga attgcctggt gctgaaagat tatccggctg aaaaagttgg tggtgttctg     3120

aatccgtatc agctgaccga tcagtttacc agctttgcaa aaatgggcac ccagagcgga     3180

tttctgtttt atgttccggc accgtatacg agcaaaattg atccgctgac cggttttgtt     3240

gatccgtttg tttggaaaac catcaaaaac catgaaagcc gcaaacattt tctggaaggt     3300

ttcgattttc tgcattacga cgttaaaacg ggtgatttca tcctgcactt taaaatgaat     3360

cgcaatctga gttttcagcg tggcctgcct ggttttatgc ctgcatggga tattgtgttt     3420

gagaaaaacg aaacacagtt cgatgcaaaa ggcaccccgt ttattgcagg taaacgtatt     3480

gttccggtga ttgaaaatca tcgtttcacc ggtcgttatc gcgatctgta tccggcaaat     3540

gaactgatcg cactgctgga agagaaaggt attgtttttc gtgatggctc aaacattctg     3600

ccgaaactgc tggaaaatga tgatagccat gcaattgata ccatggttgc actgattcgt     3660

agcgttctgc agatgcgtaa tagcaatgca gcaaccggtg aagattacat taatagtccg     3720

gttcgtgatc tgaatggtgt ttgttttgat agccgttttc agaatccgga atggccgatg     3780

gatgcagatg caaatggtgc atatcatatt gcactgaaag gacagctgct gctgaaccac     3840

ctgaaagaaa gcaaagatct gaaactgcaa aacggcatta gcaatcagga ttggctggca     3900

tatatccaag aactgcgtaa cggtcgtagc agtgatgatg aagcaaccgc agatagccag     3960

catgcagcac cgcctaaaaa gaaacgtaaa gttggtggta gcggtggttc aggtggtagt     4020

ggcggtagtg gtggctcagg gggttctggt ggctctggtg gtagcctcga gcaccaccac     4080

caccaccac                                                             4089


<210>  1117
<211>  4083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cpf1


<220>
<221>  misc_feature
<222>  (4024)..(4044)
<223>  NLS

<220>
<221>  misc_feature
<222>  (4066)..(4083)
<223>  His tag

<400>  1117
atgggtcggg atccaggtaa accgattccg aatccgctgc tgggtctgga tagcaccgca       60

ccgaaaaaaa aacgtaaagt tggtattcat ggtgttccgg cagcaaccca gtttgaaggt      120

ttcaccaatc tgtatcaggt tagcaaaacc ctgcgttttg aactgattcc gcagggtaaa      180

accctgaaac atattcaaga acagggcttc atcgaagagg ataaagcacg taacgatcac      240

tacaaagaac tgaaaccgat tatcgaccgc atctataaaa cctatgcaga tcagtgtctg      300

cagctggttc agctggattg ggaaaatctg agcgcagcaa ttgatagtta tcgcaaagaa      360

aaaaccgaag aaacccgtaa tgcactgatt gaagaacagg caacctatcg taatgccatc      420

catgattatt tcattggtcg taccgataat ctgaccgatg caattaacaa acgtcacgcc      480

gaaatctata aaggcctgtt taaagccgaa ctgtttaatg gcaaagttct gaaacagctg      540

ggcaccgtta ccaccaccga acatgaaaat gcactgctgc gtagctttga taaattcacc      600

acctatttca gcggctttta tgagaatcgc aaaaacgtgt ttagcgcaga agatattagc      660

accgcaattc cgcatcgtat tgtgcaggat aatttcccga aattcaaaga gaactgccac      720

atttttaccc gtctgattac cgcagttccg agcctgcgtg aacattttga aaacgttaaa      780

aaagccatcg gcatctttgt tagcaccagc attgaagaag tttttagctt cccgttttac      840

aatcagctgc tgacccagac ccagattgat ctgtataacc aactgctggg tggtattagc      900

cgtgaagcag gcaccgaaaa aatcaaaggt ctgaatgaag tgctgaatct ggccattcag      960

aaaaatgatg aaaccgcaca tattattgca agcctgccgc atcgttttat tccgctgttc     1020

aaacaaattc tgagcgatcg taataccctg agctttattc tggaagaatt caaatccgat     1080

gaagaggtga ttcagagctt ttgcaaatac aaaacgctgc tgcgcaatga aaatgttctg     1140

gaaactgccg aagcactgtt taacgaactg aatagcattg atctgaccca catctttatc     1200

agccacaaaa aactggaaac catttcaagc gcactgtgtg atcattggga taccctgcgt     1260

aatgccctgt atgaacgtcg tattagcgaa ctgaccggta aaattaccaa aagcgcgaaa     1320

gaaaaagttc agcgcagtct gaaacatgag gatattaatc tgcaagagat tattagcgca     1380

gccggtaaag aactgtcaga agcatttaaa cagaaaacca gcgaaattct gtcacatgca     1440

catgcagcac tggatcagcc gctgccgacc accctgaaaa aacaagaaga aaaagaaatc     1500

ctgaaaagcc agctggatag cctgctgggt ctgtatcatc tgctggactg gtttgcagtt     1560

gatgaaagca atgaagttga tccggaattt agcgcacgtc tgaccggcat taaactggaa     1620

atggaaccga gcctgagctt ttataacaaa gcccgtaatt atgccaccaa aaaaccgtat     1680

agcgtcgaaa aattcaaact gaactttcag atgccgaccc tggcaagcgg ttgggatgtt     1740

aataaagaaa aaaacaacgg tgccatcctg ttcgtgaaaa atggcctgta ttatctgggt     1800

attatgccga aacagaaagg tcgttataaa gcgctgagct ttgaaccgac ggaaaaaacc     1860

agtgaaggtt ttgataaaat gtactacgac tattttccgg atgcagccaa aatgattccg     1920

aaatgtagca cccagctgaa agcagttacc gcacattttc agacccatac caccccgatt     1980

ctgctgagca ataactttat tgaaccgctg gaaatcacca aagagatcta cgatctgaat     2040

aacccggaaa aagagccgaa aaaattccag accgcatatg caaaaaaaac cggtgatcag     2100

aaaggttatc gtgaagcgct gtgtaaatgg attgatttca cccgtgattt tctgagcaaa     2160

tacaccaaaa ccaccagtat cgatctgagc agcctgcgtc cgagcagcca gtataaagat     2220

ctgggcgaat attatgcaga actgaatccg ctgctgtatc atattagctt tcagcgtatt     2280

gccgagaaag aaatcatgga cgcagttgaa accggtaaac tgtacctgtt ccagatctac     2340

aataaagatt ttgccaaagg ccatcatggc aaaccgaatc tgcataccct gtattggacc     2400

ggtctgttta gccctgaaaa tctggcaaaa acctcgatta aactgaatgg tcaggcggaa     2460

ctgttttatc gtccgaaaag ccgtatgaaa cgtatggcac atcgtctggg tgaaaaaatg     2520

ctgaacaaaa aactgaaaga ccagaaaacc ccgatcccgg atacactgta tcaagaactg     2580

tatgattatg tgaaccatcg tctgagccat gatctgagtg atgaagcacg tgccctgctg     2640

ccgaatgtta ttaccaaaga agttagccac gagatcatta aagatcgtcg ttttaccagc     2700

gacaaattct tttttcatgt gccgattacc ctgaattatc aggcagcaaa tagcccgagc     2760

aaatttaacc agcgtgttaa tgcatatctg aaagaacatc cagaaacgcc gattattggt     2820

attgatcgtg gtgaacgtaa cctgatttat atcaccgtta ttgatagcac cggcaaaatc     2880

ctggaacagc gtagcctgaa taccattcag cagtttgatt accagaaaaa actggataat     2940

cgcgagaaag aacgtgttgc agcacgtcag gcatggtcag ttgttggtac aattaaagac     3000

ctgaaacagg gttatctgag ccaggttatt catgaaattg tggatctgat gattcactat     3060

caggccgttg ttgtgctgga aaacctgaat tttggcttta aaagcaaacg taccggcatt     3120

gcagaaaaag cagtttatca gcagttcgag aaaatgctga ttgacaaact gaattgcctg     3180

gtgctgaaag attatccggc tgaaaaagtt ggtggtgttc tgaatccgta tcagctgacc     3240

gatcagttta ccagctttgc aaaaatgggc acccagagcg gatttctgtt ttatgttccg     3300

gcaccgtata cgagcaaaat tgatccgctg accggttttg ttgatccgtt tgtttggaaa     3360

accatcaaaa accatgaaag ccgcaaacat tttctggaag gtttcgattt tctgcattac     3420

gacgttaaaa cgggtgattt catcctgcac tttaaaatga atcgcaatct gagttttcag     3480

cgtggcctgc ctggttttat gcctgcatgg gatattgtgt ttgagaaaaa cgaaacacag     3540

ttcgatgcaa aaggcacccc gtttattgca ggtaaacgta ttgttccggt gattgaaaat     3600

catcgtttca ccggtcgtta tcgcgatctg tatccggcaa atgaactgat cgcactgctg     3660

gaagagaaag gtattgtttt tcgtgatggc tcaaacattc tgccgaaact gctggaaaat     3720

gatgatagcc atgcaattga taccatggtt gcactgattc gtagcgttct gcagatgcgt     3780

aatagcaatg cagcaaccgg tgaagattac attaatagtc cggttcgtga tctgaatggt     3840

gtttgttttg atagccgttt tcagaatccg gaatggccga tggatgcaga tgcaaatggt     3900

gcatatcata ttgcactgaa aggacagctg ctgctgaacc acctgaaaga aagcaaagat     3960

ctgaaactgc aaaacggcat tagcaatcag gattggctgg catatatcca agaactgcgt     4020

aaccctaaaa aaaaacgcaa agtgaagctt gcggccgcac tcgagcacca ccaccaccac     4080

cac                                                                   4083


<210>  1118
<211>  304
<212>  DNA
<213>  Homo sapiens

<400>  1118
tcctaaagct tggaacactt tcccttcctt aagaaccatc cttgctactc agctgcaatc       60

aatccagccc ccaggtcttc actgaacctt ttcccatctc ttccaaaaca tctgtttctg      120

agaagtcctg tcctatagag gtctttcttc ccaccggatt tctcctacac catttactcc      180

cacttgcaga actcccgtgt acaagtgtct ttactgcttt tatttgctca tcaaaatgca      240

catctcatat aaaaataaat gaggagcatg cacacaccac aaacacaaac aggcatgcag      300

aaat                                                                   304


<210>  1119
<211>  665
<212>  DNA
<213>  Homo sapiens

<400>  1119
ataaagatga acccatagtg agctgagagc tccagcctgg cctccagata actacacacc       60

aagcttccac ccagaatcaa gcctatgtta acttccctca aagcctgaga ttttgccttc      120

ccattaaatg caggtagttg ttccccttca agcactagtc actggccata atttaaatct      180

tgctatcttc ttgccaccat gaaccctgta tgttgtaggc tgaagacgtt aaaagaaaca      240

cacgctgaca cacacacaca cacgcgcgcg cgcacacaca cacacacaca cagagctgac      300

tttcaaaatc tactccagcc caaatgtttc aattgttcct cacccctgga catactttgc      360

ccccatctgg aattaaagga tataagtttg taatgaagca ttagcagcat tttatatgtg      420

tccagctgat ataggaatag ccttagcaat gtatgtttgg ccaccaaagt tccccacttt      480

gactgagcca atatatgcct tctgcctgca tctttttaac gaccatactt gtcctgcctc      540

cagatagatg ttttaaaaca acaaaaatga gggaaagatg aaagttcttt ctactggaat      600

ctaataaaga aaagtcattt tcctcatttc cacctctctt ttctcaaagt caaaattgtc      660

catct                                                                  665


<210>  1120
<211>  165
<212>  DNA
<213>  Homo sapiens

<400>  1120
ccctaaaaca ttaccactgg gtctcagccc agttagtcct ctgcagtttc ttcaccccca       60

accccagtat cttcaaacag ctcacaccct gctgtgctca gatcaatact ccgttgtcta      120

agttgcctcg agactaaagg caacagggct gaaacatctc ctgga                      165


<210>  1121
<211>  122
<212>  DNA
<213>  Homo sapiens

<400>  1121
ctgtgagatt gacaagaaca gtttgacagt cagaaggtgc cacaaatcct gagaagcgac       60

ctggactttt gccaggcaca gggtccttcc ttccctccct tgtcctggtc accagagcct      120

ac                                                                     122


<210>  1122
<211>  24
<212>  DNA
<213>  Homo sapiens

<400>  1122
gccgccggcc cctggcctca ctgg                                              24


<210>  1123
<211>  33
<212>  DNA
<213>  Homo sapiens

<400>  1123
ccttgtcaag gctattggtc aaggcaaggc tgg                                    33


<210>  1124
<211>  36
<212>  DNA
<213>  Homo sapiens

<400>  1124
tgagatagtg tggggaaggg gcccccaaga ggatac                                 36


<210>  1125
<211>  41
<212>  DNA
<213>  Homo sapiens

<400>  1125
tatagccttt gccttgttcc gattcagtca ttccagtttt t                           41


<210>  1126
<211>  114
<212>  DNA
<213>  Homo sapiens

<400>  1126
tcttcccttt agctagtttc cttctcccat catagaggat accaggactt cttttgtcag       60

ccgtttttta ccttcttgtc tctagctcca gtgaggcctg tagtttaaag ctaa            114


<210>  1127
<211>  86
<212>  DNA
<213>  Homo sapiens

<400>  1127
ccacagtttc agcgcagtaa tagattagtg ttacataata taagacctaa tgcttacctc       60

aatatctact tatccgtacc tatttg                                            86


<210>  1128
<211>  142
<212>  DNA
<213>  Homo sapiens

<400>  1128
tattcaggta tgtatgtata caccagatga tgtgtattta ccactggata agtgtgtgtg       60

ctggctgatg acccagggtt ttggcgtagc tcttctatgc tcagtaaaga tgatggtaga      120

atgttctttg gcaggtactg tg                                               142


<210>  1129
<211>  697
<212>  DNA
<213>  Homo sapiens

<400>  1129
caataaagat gaacccatag tgagctgaga gctccagcct ggcctccaga taactacaca       60

ccaagcttcc acccagaatc aagcctatgt taacttccct caaagcctga gattttgctt      120

tcccattaaa tgcaggtagt tgttcttctt gcagcactag tcactggcca taatttaaat      180

cttgttatct tcttgccacc atgaaccctg tatgctgtag gctgaaaacg ttaaaagaaa      240

cacacgctct cacacacaca caaacacacg cgcgcacaca cacacacaca cacacagagc      300

tgactttcaa aatctactcc agcccaaatg tttcaattgt tcctcacccc tggacatact      360

ttgcccccat ctggaattaa aggatataag tttgtaatga agcattagca gcattttata      420

tgtgtccagc tgatatagga atagccttag caatgtatgt ttggccacca aagttcccca      480

ctttgactga gccaatatat gccttctgcc tgcatctttt taatgaccat acttgtcctg      540

cctccagata gatgttttaa aacgaataac aaaaataggg gaaaggtgaa agttctttct      600

accgaaatct aataaagaaa agtcattttc ctcatttcca cctctctttt ctcaaagtca      660

aagttgtcca tctagatttt cagaggcact ccttagg                               697


<210>  1130
<211>  185
<212>  DNA
<213>  Homo sapiens

<400>  1130
ccctaaaaca ttgccactgg gtctcagccc agttagtcct ctgcagtttc ttcactccca       60

accccagtat cttcaaacag ctcacaccct gctgtgctca gatcaatact cagttgtcta      120

agttgcctcg agactaaagg caacagtgct gaaacatctc ctggactcac cttgaagttc      180

tcagg                                                                  185


<210>  1131
<211>  137
<212>  DNA
<213>  Homo sapiens

<400>  1131
agcctgtgag attgacaaga acagtttgac agtcagaagg tgccacaaat cctgagaagc       60

gacctggact tttgccaggc acagggtcct tccttccctc ccttgtcctg gtcaccagag      120

cctaccttcc cagggtt                                                     137


<210>  1132
<211>  38
<212>  DNA
<213>  Homo sapiens

<400>  1132
ccgccggccc ctggcctcac tggatactct aagactat                               38


<210>  1133
<211>  31
<212>  DNA
<213>  Homo sapiens

<400>  1133
ccttgtcaag gctattggtc aaggcaaggc t                                      31


<210>  1134
<211>  75
<212>  DNA
<213>  Homo sapiens

<400>  1134
cagggaccgt ttcagacaga tatttgcatt gagatagtgt ggggaagggg cccccaagag       60

gatactgctg cttaa                                                        75


<210>  1135
<211>  29
<212>  DNA
<213>  Homo sapiens

<400>  1135
ttgccttgtt ccgattcagt cattccaat                                         29


<210>  1136
<211>  104
<212>  DNA
<213>  Homo sapiens

<400>  1136
tttagctagt tttcttctcc caccatagaa gataccagga cttcttttgt cagccgtttt       60

tcaccttctt gtctgtagct ccagtgaggc ctgtagttta aagt                       104


<210>  1137
<211>  38
<212>  DNA
<213>  Homo sapiens

<400>  1137
ggacacgtct tagtctcatt tagtaagcat tggtttcc                               38


<210>  1138
<211>  124
<212>  DNA
<213>  Homo sapiens

<400>  1138
ttttttatat tcaggtatgt atgtaggcac ccgatgatgt gtatttatca ctggataagt       60

gtatgtgctg gctgatgacc cagggttttg gtgtagctct tctatgctcg gtaaagatga      120

tggt                                                                   124


<210>  1139
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-1

<400>  1139
agacagauau uugcauugag                                                   20


<210>  1140
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-1

<400>  1140
agacagatat ttgcattgag                                                   20


<210>  1141
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-2

<400>  1141
cauugagaua guguggggaa                                                   20


<210>  1142
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-2

<400>  1142
cattgagata gtgtggggaa                                                   20


<210>  1143
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-3

<400>  1143
uagccuuugc cuuguuccga                                                   20


<210>  1144
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-3

<400>  1144
tagcctttgc cttgttccga                                                   20


<210>  1145
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-4

<400>  1145
ccuuguuccg auucagucau                                                   20


<210>  1146
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-4

<400>  1146
ccttgttccg attcagtcat                                                   20


<210>  1147
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-5

<400>  1147
ucuaauuuau ucuucccuuu                                                   20


<210>  1148
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-5

<400>  1148
tctaatttat tcttcccttt                                                   20


<210>  1149
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-6

<400>  1149
cuucucccau cauagaggau                                                   20


<210>  1150
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-6

<400>  1150
cttctcccat catagaggat                                                   20


<210>  1151
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG2 Promoter-7

<400>  1151
uucucccacc auagaagaua                                                   20


<210>  1152
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG2 Promoter-7

<400>  1152
ttctcccacc atagaagata                                                   20


<210>  1153
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-8

<400>  1153
ccacuggaua agugugugug                                                   20


<210>  1154
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-8

<400>  1154
ccactggata agtgtgtgtg                                                   20


<210>  1155
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-9

<400>  1155
gcguagcucu ucuaugcuca                                                   20


<210>  1156
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-9

<400>  1156
gcgtagctct tctatgctca                                                   20


<210>  1157
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-10

<400>  1157
cugagcauag aagagcuacg                                                   20


<210>  1158
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG1 Promoter-10

<400>  1158
ctgagcatag aagagctacg                                                   20


<210>  1159
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG2 Promoter-11

<400>  1159
ucacuggaua aguguaugug                                                   20


<210>  1160
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG2 Promoter-11

<400>  1160
tcactggata agtgtatgtg                                                   20


<210>  1161
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG2 Promoter-12

<400>  1161
guguagcucu ucuaugcucg                                                   20


<210>  1162
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG2 Promoter-12

<400>  1162
gtgtagctct tctatgctcg                                                   20


<210>  1163
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG2 Promoter-13

<400>  1163
ccgagcauag aagagcuaca                                                   20


<210>  1164
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 HBG2 Promoter-13

<400>  1164
ccgagcatag aagagctaca                                                   20


<210>  1165

<400>  1165
000

<210>  1166

<400>  1166
000

<210>  1167
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-1

<400>  1167
gacagauauu ugcauugaga                                                   20


<210>  1168
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-1

<400>  1168
gacagatatt tgcattgaga                                                   20


<210>  1169
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-2

<400>  1169
acacuaucuc aaugcaaaua                                                   20


<210>  1170
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-2

<400>  1170
acactatctc aatgcaaata                                                   20


<210>  1171
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-3

<400>  1171
cacacuaucu caaugcaaau                                                   20


<210>  1172
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-3

<400>  1172
cacactatct caatgcaaat                                                   20


<210>  1173
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-4

<400>  1173
ccacacuauc ucaaugcaaa                                                   20


<210>  1174
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-4

<400>  1174
ccacactatc tcaatgcaaa                                                   20


<210>  1175
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-5

<400>  1175
uuccccacac uaucucaaug                                                   20


<210>  1176
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-5

<400>  1176
ttccccacac tatctcaatg                                                   20


<210>  1177
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-6

<400>  1177
gauucaguca uuccaguuuu                                                   20


<210>  1178
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-6

<400>  1178
gattcagtca ttccagtttt                                                   20


<210>  1179
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-7

<400>  1179
auucagucau uccaguuuuu                                                   20


<210>  1180
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-7

<400>  1180
attcagtcat tccagttttt                                                   20


<210>  1181
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-8

<400>  1181
gucauuccag uuuuucucua                                                   20


<210>  1182
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-8

<400>  1182
gtcattccag tttttctcta                                                   20


<210>  1183
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-9

<400>  1183
aguuuuucuc uaauuuauuc                                                   20


<210>  1184
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-9

<400>  1184
agtttttctc taatttattc                                                   20


<210>  1185
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-10

<400>  1185
guuuuucucu aauuuauucu                                                   20


<210>  1186
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-10

<400>  1186
gtttttctct aatttattct                                                   20


<210>  1187
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-11

<400>  1187
gauucaguca uuccaauuuu                                                   20


<210>  1188
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-11

<400>  1188
caagaggata ctgctgctta                                                   20


<210>  1189
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-12

<400>  1189
auucagucau uccaauuuuu                                                   20


<210>  1190
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-12

<400>  1190
aagaggatac tgctgcttaa                                                   20


<210>  1191
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-13

<400>  1191
gucauuccaa uuuuucucua                                                   20


<210>  1192
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-13

<400>  1192
gattcagtca ttccaatttt                                                   20


<210>  1193
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-14

<400>  1193
aauuuuucuc uaauuuauuc                                                   20


<210>  1194
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-14

<400>  1194
attcagtcat tccaattttt                                                   20


<210>  1195
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-15

<400>  1195
auuuuucucu aauuuauucu                                                   20


<210>  1196
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-15

<400>  1196
gtcattccaa tttttctcta                                                   20


<210>  1197
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-16

<400>  1197
uucucccauc auagaggaua                                                   20


<210>  1198
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-16

<400>  1198
aatttttctc taatttattc                                                   20


<210>  1199
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-17

<400>  1199
aucauagagg auaccaggac                                                   20


<210>  1200
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-17

<400>  1200
atttttctct aatttattct                                                   20


<210>  1201
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-18

<400>  1201
accauagaag auaccaggac                                                   20


<210>  1202
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-18

<400>  1202
ttctcccatc atagaggata                                                   20


<210>  1203
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-19

<400>  1203
caguaccugc caaagaacau                                                   20


<210>  1204
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-19

<400>  1204
atcatagagg ataccaggac                                                   20


<210>  1205
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-20

<400>  1205
uaguaucugg uaaagagcau                                                   20


<210>  1206
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-20

<400>  1206
accatagaag ataccaggac                                                   20


<210>  1207
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-21

<400>  1207
ucaaugcaaa uaucugucug                                                   20


<210>  1208
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG1 Promoter-21

<400>  1208
cagtacctgc caaagaacat                                                   20


<210>  1209
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-22

<400>  1209
cucuuggggg ccccuucccc                                                   20


<210>  1210
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RR HBG2 Promoter-22

<400>  1210
tagtatctgg taaagagcat                                                   20


<210>  1211
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-1

<400>  1211
gauucaguca uuccaauuuu                                                   20


<210>  1212
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-1

<400>  1212
tcaatgcaaa tatctgtctg                                                   20


<210>  1213
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-2

<400>  1213
auucagucau uccaauuuuu                                                   20


<210>  1214
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-2

<400>  1214
ctcttggggg ccccttcccc                                                   20


<210>  1215
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-3

<400>  1215
aaaaaaauua gcaguauccu                                                   20


<210>  1216
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-3

<400>  1216
aaaaaaatta gcagtatcct                                                   20


<210>  1217
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-4

<400>  1217
gccuuugccu uguuccgauu                                                   20


<210>  1218
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-4

<400>  1218
gcctttgcct tgttccgatt                                                   20


<210>  1219
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-5

<400>  1219
aaaaaaauua agcagcagua                                                   20


<210>  1220
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-5

<400>  1220
aaaaaaatta agcagcagta                                                   20


<210>  1221
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-6

<400>  1221
cucaguaaag augaugguag                                                   20


<210>  1222
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG1 Promoter-6

<400>  1222
ctcagtaaag atgatggtag                                                   20


<210>  1223
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-7

<400>  1223
acuggauaag uguaugugcu                                                   20


<210>  1224
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-7

<400>  1224
actggataag tgtatgtgct                                                   20


<210>  1225
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-8

<400>  1225
ugcuggcuga ugacccaggg                                                   20


<210>  1226
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-8

<400>  1226
tgctggctga tgacccaggg                                                   20


<210>  1227
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-9

<400>  1227
cucgguaaag augaugguag                                                   20


<210>  1228
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-9

<400>  1228
ctcggtaaag atgatggtag                                                   20


<210>  1229
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-10

<400>  1229
ugguaaagag cauucuacca                                                   20


<210>  1230
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting domain - AsCpf1 RVR HBG2 Promoter-10

<400>  1230
tggtaaagag cattctacca                                                   20


<210>  1231
<211>  5
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  +5 RNA gRNA 5' extension

<400>  1231
cuuuu                                                                    5


<210>  1232
<211>  10
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  +10 RNA gRNA 5' extension

<400>  1232
aagaccuuuu                                                              10


<210>  1233
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  +25 RNA gRNA 5' extension

<400>  1233
auguguuuuu gucaaaagac cuuuu                                             25


<210>  1234
<211>  60
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  +60 RNA gRNA 5' extension

<400>  1234
aggccagcuu gccgguuuuu uagucgugcu gcuucaugug uuuuugucaa aagaccuuuu       60


<210>  1235
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +5 DNA gRNA 5' extension

<400>  1235
ctttt                                                                    5


<210>  1236
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +10 DNA gRNA 5' extension

<400>  1236
aagacctttt                                                              10


<210>  1237
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +25 DNA gRNA 5' extension

<400>  1237
atgtgttttt gtcaaaagac ctttt                                             25


<210>  1238
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +60 DNA gRNA 5' extension

<400>  1238
aggccagctt gccggttttt tagtcgtgct gcttcatgtg tttttgtcaa aagacctttt       60


<210>  1239
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +20 DNA gRNA 5' extension

<400>  1239
tttttgtcaa aagacctttt                                                   20


<210>  1240
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +30 DNA gRNA 5' extension

<400>  1240
gcttcatgtg tttttgtcaa aagacctttt                                        30


<210>  1241
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +50 DNA gRNA 5' extension

<400>  1241
gccggttttt tagtcgtgct gcttcatgtg tttttgtcaa aagacctttt                  50


<210>  1242
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +40 DNA gRNA 5' extension

<400>  1242
tagtcgtgct gcttcatgtg tttttgtcaa aagacctttt                             40


<210>  1243
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +20 DNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1243
ccgaagtttt cttcggtttt                                                   20


<210>  1244
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +25 DNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1244
tttttccgaa gttttcttcg gtttt                                             25


<210>  1245
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +30 DNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1245
aacgcttttt ccgaagtttt cttcggtttt                                        30


<210>  1246
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +41 DNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1246
gcgttgtttt caacgctttt tccgaagttt tcttcggttt t                           41


<210>  1247
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +62 DNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1247
ggcttctttt gaagcctttt tgcgttgttt tcaacgcttt ttccgaagtt ttcttcggtt       60

tt                                                                      62


<210>  1248
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +25 DNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1248
atgtgttttt gtcaaaagac ctttt                                             25


<210>  1249
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +25 A gRNA 5' extension

<400>  1249
aaaaaaaaaa aaaaaaaaaa aaaaa                                             25


<210>  1250
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  +25 T gRNA 5' extension

<400>  1250
tttttttttt tttttttttt ttttt                                             25


<210>  1251
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  +25 RNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1251
auguguuuuu gucaaaagac cuuuu                                             25


<210>  1252
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  +25 polyA RNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1252
aaaaaaaaaa aaaaaaaaaa aaaaa                                             25


<210>  1253
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  +25 polyU RNA/2xPS gRNA 5' extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linked by phosphorothioate (PS) bond

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Modified to contain 2'-O-methyl

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Linked by phosphorothioate (PS) bond

<400>  1253
uuuuuuuuuu uuuuuuuuuu uuuuu                                             25


<210>  1254
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 21mer targeting domain

<400>  1254
ccuugucaag gcuauugguc a                                                 21


<210>  1255
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 21mer targeting domain

<400>  1255
ccttgtcaag gctattggtc a                                                 21


<210>  1256
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 22mer targeting domain

<400>  1256
ccuugucaag gcuauugguc aa                                                22


<210>  1257
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 22mer targeting domain

<400>  1257
ccttgtcaag gctattggtc aa                                                22


<210>  1258
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 23mer targeting domain

<400>  1258
ccuugucaag gcuauugguc aag                                               23


<210>  1259
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HBG1-1 23mer targeting domain

<400>  1259
ccttgtcaag gctattggtc aag                                               23


<210>  1260
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AsCpf1 HBG1 promoter-1 21mer targeting domain

<400>  1260
agacagauau uugcauugag a                                                 21


<210>  1261
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AsCpf1 HBG1 promoter-1 21mer targeting domain

<400>  1261
agacagatat ttgcattgag a                                                 21


<210>  1262
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AsCpf1 HBG1 promoter-2 21mer targeting domain

<400>  1262
cauugagaua guguggggaa g                                                 21


<210>  1263
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AsCpf1 HBG1 promoter-2 21mer targeting domain

<400>  1263
cattgagata gtgtggggaa g                                                 21


<210>  1264
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  AsCpf1 HBG1 promoter-6 21mer targeting domain

<400>  1264
cuucucccau cauagaggau a                                                 21


<210>  1265
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AsCpf1 HBG1 promoter-6 21mer targeting domain

<400>  1265
cttctcccat catagaggat a                                                 21


